Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,127,430	C→A	A198S (GCG→TCG)	glcD ←	glycolate dehydrogenase, putative FAD‑linked subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,127,430	0	C	A	100.0%	229.1 / NA	64	A198S (GCG→TCG)	glcD	glycolate dehydrogenase, putative FAD‑linked subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (33/31);  total (33/31)

AGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGTTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAATCCAGCGCGTCCGATCCAAGCGTCAGTGCCTCGCCGTCCAGCGTTTGCACTTCAATTTTCAGCAGGTTATGTACGGTCAGACCATATTTCAGGCAGTGGACGCCGCCGGCATTTTCAGCCACATTGCCGCCAATGGAA  >  NC_000913/3127295‑3127560                                                                                                                                        |                                                                                                                                   aGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGTTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAATCCAGCGAGTcc                                                                                                                                >  2:1972899/1‑140 (MQ=255) aGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGTTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAATCCAGCGAGTcc                                                                                                                                >  2:1127631/1‑140 (MQ=255) aGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGTTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAATCCAGCGAGTcc                                                                                                                                >  2:1185175/1‑140 (MQ=255)            gCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGTTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAATCCAGCGAGTCCGATCCAAGCGt                                                                                                                     >  2:938342/1‑140 (MQ=255)            gCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGTTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAATCCAGCGAGTCCGATCCAAGCGt                                                                                                                     >  2:1109452/1‑140 (MQ=255)             cTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGTTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAATCCAGCGAGTCCGATCCAAGCGTc                                                                                                                    >  1:1951184/1‑140 (MQ=255)                 cAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGTTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCCGCAGGTCAAAAACAGGGGACTCCCGCGAGTCCCATCCAAGCGTCACTg                                                                                                                >  2:391131/1‑140 (MQ=255)                 cAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGTTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAATCCAGCGAGTCCGATCCAAGCGTCAGTg                                                                                                                <  1:1316024/140‑1 (MQ=255)                      cccGCGCCACGGGCGGCTTCGGCAGCAGTTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAATCCAGCGAGTCCGATCCAAGCGTCAGTGCCTCg                                                                                                           >  1:473473/1‑140 (MQ=255)                      cccGCGCCACGGGCGGCTTCGGCAGCAGTTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAATCCAGCGAGTCCGATCCAAGCGTCAGTGCCTCg                                                                                                           <  1:129800/140‑1 (MQ=255)                      cccGCGCCACGGGCGGCTTCGGCAGCAGTTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAATCCAGCGAGTCCGATCCAAGCGTCAGTGCCTCg                                                                                                           >  2:17925/1‑140 (MQ=255)                         gcgcCACGGGCGGCTTCGGCAGCAGTTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAATCCAGCGAGTCCGATCCAAGCGTCAGTGCCTCGCCg                                                                                                        >  2:855084/1‑140 (MQ=255)                          cgcCACGGGCGGCTTCGGCAGCAGTTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAATCCAGCGAGTCCGATCCAAGCGTCAGTGCCTCGCCGt                                                                                                       >  2:972005/1‑140 (MQ=255)                          cgcCACGGGCGGCTTCGGCAGCAGTTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAATCCAGCGAGTCCGATCCAAGCGTCAGTGCCTCGCCGt                                                                                                       >  1:639964/1‑140 (MQ=255)                          cgcCACGGGCGGCTTCGGCAGCAGTTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAATCCAGCGAGTCCGATCCAAGCGTCAGTGCCTCGCCGt                                                                                                       >  2:1464424/1‑140 (MQ=255)                                     gcTTCGGCAGCAGTTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAATCCAGCGAGTCCGATCCAAGCGTCAGTGCCTCGCCGTCCAGCGTTTGc                                                                                            >  1:1558021/1‑140 (MQ=255)                                         cGGCAGCAGTTTTACCGTCCCTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAATCCAGCGAGTCCGATCCAAGCGTCAGTGCCTCGCCGTCCAGCGTTTGCACtt                                                                                        <  2:473473/140‑1 (MQ=255)                                              gcagTTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAATCCAGCGAGTCCGATCCAAGCGTCAGTGCCTCGCCGTCCAGCGTTTGCACTTCAAtt                                                                                   <  1:938342/140‑1 (MQ=255)                                                   tttACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAATCCAGCGAGTCCGATCCAAGCGTCAGTGCCTCGCCGTCCAGCGTTTGCACTTCAATTTTcag                                                                              <  2:1580987/140‑1 (MQ=255)                                                   tttACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAATCCAGCGAGTCCGATCCAAGCGTCAGTGCCTCGCCGTCCAGCGTTTGCACTTCAATTTTcag                                                                              <  2:502632/140‑1 (MQ=255)                                                   tttACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAATCCAGCGAGTCCGATCCAAGCGTCAGTGCCTCGCCGTCCAGCGTTTGCACTTCAATTTTcag                                                                              <  1:550833/140‑1 (MQ=255)                                                   tttACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAATCCAGCGAGTCCGATCCAAGCGTCAGTGCCTCGCCGTCCAGCGTTTGCACTTCAATTTTcag                                                                              <  1:1127631/140‑1 (MQ=255)                                                   tttACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAATCCAGCGAGTCCGATCCAAGCGTCAGTGCCTCGCCGTCCAGCGTTTGCACTTCAATTTTcag                                                                              <  1:1466571/140‑1 (MQ=255)                                                    ttACCGTCACTTCGGTGGTCACGCCGAGCATGCCTTCCGAGCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAATCCAGCGAGTCCGATCCAAGCGTCAGTGCCTCGCCGTCCAGCGTTTGCACTTCAATTTTcagc                                                                             <  2:1312476/140‑1 (MQ=255)                                                    ttACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAATCCAGCGAGTCCGATCCAAGCGTCAGTGCCTCGCCGTCCAGCGTTTGCACTTCAATTTTcagc                                                                             <  1:1109452/140‑1 (MQ=255)                                                    ttACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAATCCAGCGAGTCCGATCCAAGCGTCAGTGCCTCGCCGTCCAGCGTTTGCACTTCAATTTTcagc                                                                             <  1:1972899/140‑1 (MQ=255)                                                    ttACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAATCCAGCGAGTCCGATCCAAGCGTCAGTGCCTCGCCGTCCAGCGTTTGCACTTCAATTTTcagc                                                                             <  1:652317/140‑1 (MQ=255)                                                    ttACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAATCCAGCGAGTCCGATCCAAGCGTCAGTGCCTCGCCGTCCAGCGTTTGCACTTCAATTTTcagc                                                                             <  2:1583990/140‑1 (MQ=255)                                                    ttACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAATCCAGCGAGTCCGATCCAAGCGTCAGTGCCTCGCCGTCCAGCGTTTGCACTTCAATTTTcagc                                                                             <  1:1771059/140‑1 (MQ=255)                                                      aCCGTCACTTCGGTGGTCACGCCGAGCATCCCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAATCCAGCGAGTCCGATCCAAGCGTCAGTGCCTCGCCGTCCAGCGTTTGCACTTCAATTTTcagcag                                                                           <  1:460175/140‑1 (MQ=255)                                                       ccGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAATCCAGCGAGTCCGATCCAAGCGTCAGTGCCTCGCCGTCCAGCGTTTGCACTTCAATTTTCAGCAgg                                                                          <  2:639964/140‑1 (MQ=255)                                                        cGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAATCCAGCGAGTCCGATCCAAGCGTCAGTGCCTCGCCGTCCAGCGTTTGCACTTCAATTTTCAGCAGGt                                                                         <  1:2031025/140‑1 (MQ=255)                                                        cGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAATCCAGCGAGTCCGATCCAAGCGTCAGTGCCTCGCCGTCCAGCGTTTGCACTTCAATTTTCAGCAGGt                                                                         <  1:1391379/140‑1 (MQ=255)                                                         gTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAATCCAGCGAGTCCGATCCAAGCGTCAGTGCCTCGCCGTCCAGCGTTTGCACTTCAATTTTcagc                                                                             >  1:44019/1‑135 (MQ=255)                                                         gTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAATCCAGCGAGTCCGATCCAAGCGTCAGTGCCTCGCCGTCCAGCGTTTGCACTTCAATTTTcagc                                                                             <  2:44019/135‑1 (MQ=255)                                                              ttCGGTGGTCATGGCGAGCATACCTTCGGAGCCGGTCAACAGCGGCAGCAGGTGAAAAGCAGGAGAATTCAGCGTGTCCGATACAAGCGTCAGTGCCTCGCCGTCCAGCATTTGCACTTCAATTTTCAGCAggaactgaa                                                                   >  2:865389/1‑133 (MQ=255)                                                                    ggtCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAATCCAGCGAGTCCGATCCAAGCGTCAGTGCCTCGCCGTCCAGCGTTTGCACTTCAATTTTCAGCAGGTTATGTACGGTCa                                                             <  2:1558021/140‑1 (MQ=255)                                                                        aCGCCGAGTATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAATCCAGCGAGTCCGATCCAAGCGTCAGTGCCTCGCCGTCCAGCGTTTGCACTTCAATTTTCAGCAGGTTATGTACGGTCAGAcc                                                         <  1:1791887/140‑1 (MQ=255)                                                                        aCGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAATCCAGCGAGTCCGATCCAAGCGTCAGTGCCTCGCCGTCCAGCGTTTGCACTTCAATTTTCAGCAGGTTATGTACGGTCAGAcc                                                         <  2:833758/140‑1 (MQ=255)                                                                                cATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAATCCAGCGAGTCCGATCCAAGCGTCAGTGCCTCGCCGTCCAGCGTTTGCACTTCAATTTTCAGCAGGTTATGTACGGTCAGACCATATTTCa                                                 >  2:1650766/1‑140 (MQ=255)                                                                                     cTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACAAGGTGAATCCAGCGAGTCCGATCCAAGCGTCAGTGCCTCGCCGTCCAGCGTTTGCACTTCAATTTTCAGCAGGTTATGTACGGTCAGACCATATTTCAGGCAg                                            >  2:572362/1‑140 (MQ=255)                                                                                        ccGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAATCCAGCGAGTCCGATCCAAGCGTCAGTGCCTCGCCGTCCAGCGTTTGCACTTCAATTTTCAGCAGGTTATGTACGGTCAGACCATATTTCAGGCAGTgg                                         >  1:2035704/1‑140 (MQ=255)                                                                                        ccGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAATCCAGCGAGTCCGATCCAAGCGTCAGTGCCTCGCCGTCCAGCGTTTGCACTTCAATTTTCAGCAGGTTATGTACGGTCAGACCATATTTCAGGCAGTgg                                         >  1:1218190/1‑140 (MQ=255)                                                                                          gATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAATCCAGCGAGTCCGATCCAAGCGTCAGTGCCTCGCCGTCCAGCGTTTGCACTTCAATTTTCAGCAGGTTATGTACGGTCAGACCATATTTCAGGCAGTGGAc                                       >  1:486847/1‑140 (MQ=255)                                                                                          gATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAATCCAGCGAGTCCGATCCAAGCGTCAGTGCCTCGCCGTCCAGCGTTTGCACTTCAATTTTCAGCAGGTTATGTACGGTCAGACCATATTTCAGGCAGTGGAc                                       >  1:63803/1‑140 (MQ=255)                                                                                           aTCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAATCCAGCGAGTCCGATCCAAGCGTCAGTGCCTCGCCGTCCAGCGTTTGCACTTCAATTTTCAGCAGGTTATGTACGGTCAGACCATATTTCAGGCAGTGGAcg                                      >  1:1137325/1‑140 (MQ=255)                                                                                             ccGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAATCCAGCGAGTCCGATCCAAGCGTCAGTGCCTCGCCGTCCAGCGTTTGCACTTCAATTTTCAGCAGGTTATGTACGGTCAGACCATATTTCAGGCAGTGGAcgcc                                    <  1:1283165/140‑1 (MQ=255)                                                                                             ccGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAATCCAGCGAGTCCGATCCAAGCGTCAGTGCCTCGCCGTCCAGCGTTTGCACTTCAATTTTCAGCAGGTTATGTACGGTCAGACCATATTTCAGGCAGTGGAcgcc                                    >  2:726716/1‑140 (MQ=255)                                                                                              cGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAATCCAGCGAGTCCGATCCAAGCGTCAGTGCCTCGCCGTCCAGCGTTTGCACTTCAATTTTCAGCAGGTTATGTACGGTCAGACCATATTTCAGGCAGTGGAcgccg                                   <  2:1452513/140‑1 (MQ=255)                                                                                               ggTGAACAGCGCCAGCAGGTCAAAACCAGGTGAATCCAGCGAGTCCGATCCAAGCGTCAGTGCCTCGCCGTCCAGCGTTTGCACTTCAATTTTCAGCAGGTTATGTACGGTCAGACCATATTTCAGGCAGTGGAcgccgc                                  >  2:1692130/1‑140 (MQ=255)                                                                                               ggTGAACAGCGCCAGCAGGTCAAAACCAGGTGAATCCAGCGAGTCCGATCCAAGCGTCAGTGCCTCGCCGTCCAGCGTTTGCACTTCAATTTTCAGCAGGTTATGTACGGTCAGACCATATTTCAGGCAGTGGAcgccgc                                  >  1:1567182/1‑140 (MQ=255)                                                                                                gTGAACAGCGCCAGCAGGTCAAAACCAGGTGAATCCAGCGAGTCCGATCCAAGCGTCAGTGCCTCGCCGTCCAGCGTTTGCACTTCAATTTTCAGCAGGTTATGTACGGTCAGACCATATTTCAGGCAGTGGACgccgcc                                 >  2:1883759/1‑140 (MQ=255)                                                                                                gTGAACAGCGCCAGCAGGTCAAAACCAGGTGAATCCAGCGAGTCCGATCCAAGCGTCAGTGCCTCGCCGTCCAGCGTTTGCACTTCAATTTTCAGCAGGTTATGTACGGTCAGACCATATTTCAGGCAGTGGACgccgcc                                 >  2:944655/1‑140 (MQ=255)                                                                                                gTGAACAGCGCCAGCAGGTCAAAACCAGGTGAATCCAGCGAGTCCGATCCAAGCGTCAGTGCCTCGCCGTCCAGCGTTTGCACTTCAATTTTCAGCAGGTTATGTACGGTCAGACCATATTTCAGGCAGTGGACgccgcc                                 >  2:1646665/1‑140 (MQ=255)                                                                                                        cgcCAGCAGGTCAAAACCAGGTGAATCCAGCGAGTCCGATCCAAGCGTCAGTGCCTCGCCGTCCAGCGTTTGCACTTCAATTTTCAGCAGGTTATGTACGGTCAGACCATATTTCAGGCAGTGGACGCCGCCGGCAtttt                         >  2:954218/1‑140 (MQ=255)                                                                                                          ccAGCAGGTCAAAACCAGGTGAATCCAGCGAGTCCGATCCAAGCGTCAGTGCCTCGCCGTCCAGCGTTTGCACTTCAATTTTCAGCAGGTTATGTACGGTCAGACCATATTTCAGGCAGTGGACGCCGCCGGCATTTTCa                       >  1:1425264/1‑140 (MQ=255)                                                                                                                ggTCAAAACCAGGTGAATCCAGCGAGTCCGATCCAAGCGTCAGTGCCTCGCCGTCCAGCGTTTGCACTTCAATTTTCAGCAGGTTATGTACGGTCAGACCATATTTCAGGCAGTGGACGCCGCCGGCATTTTCAGCcaca                 <  2:1115199/140‑1 (MQ=255)                                                                                                                  tCAAAACCAGGTGAATCCAGCGAGTCCGATCCAAGCGTCAGTGCCTCGCCGTCCAGCGTTTGCACTTCAATTTTCAGCAGGTTATGTACGGTCAGACCATATTTCAGGCAGTGGACGCCGCCGGCATTTTCAGCCACAtt               >  1:119370/1‑140 (MQ=255)                                                                                                                    aaaaCCAGGTGAATCCAGCGAGTCCGATCCAAGCGTCAGTGCCTCGCCGTCCAGCGTTTGCACTTCAATTTTCAGCAGGTTATGTACGGTCAGACCATATTTCAGGCAGTGGACGCCGCCGGCATTTTCAGCCACATTgc             <  2:599676/140‑1 (MQ=255)                                                                                                                     aaaCCAGGTGAATCCAGCGAGTCCGATCCAAGCGTCAGTGCCTCGCCGTCCAGCGTTTGCACTTCAATTTTCAGCAGGTTATGTACGGTCAGACCATATTTCAGGCAGTGGACGCCGCCGGCATTTTCAGCCACATTgcc            <  1:1185175/140‑1 (MQ=255)                                                                                                                     aaaCCAGGTGAATCCAGCGAGTCCGATCCAAGCGTCAGTGCCTCGCCGTCCAGCGTTTGCACTTCAATTTTCAGCAGGTTATGTACGGTCAGACCATATTTCAGGCAGTGGACGCCGCCGGCATTTTCAGCCACATTgcc            <  1:1175719/140‑1 (MQ=255)                                                                                                                       aCCAGGTGAATCCAGCGAGTCCGATCCAAGCGTCAGTGCCTCGCCGTCCAGCGTTTGCACTTCAATTTTCAGCAGGTTATGTACGGTCAGACCATATTTCAGGCAGTGGACGCCGCCGGCATTTTCAGCCACATTgccgc          <  2:1951184/140‑1 (MQ=255)                                                                                                                        ccAGGTGAATCCAGCGAGTCCGATCCAAGCGTCAGTGCCTCGCCGTCCAGCGTTTGCACTTCAATTTTCAGCAGGTTATGTACGGTCAGACCATATTTCAGGCAGTGGACGCCGCCGGCATTTTCAGCCACATTgccgcc         <  2:119370/140‑1 (MQ=255)                                                                                                                           ggTGAATCCAGCGAGTCCGATCCAAGCGTCAGTGCCTCGCCGTCCAGCGTTTGCACTTCAATTTTCAGCAGGTTATGTACGGTCAGACCATATTTCAGGCAGTGGACGCCGCCGGCATTTTCAGTCACATTGCCGCCAAt      >  2:522696/1‑140 (MQ=255)                                                                                                                           ggTGAATCCAGCGAGTCCGATCCAAGCGTCAGTGCCTCGCCGTCCAGCGTTTGCACTTCAATTTTCAGCAGGTTATGTACGGTCAGACCATATTTCAGGCAGTGGACGCCGCCGGCATTTTCAGCCACATTGCCGCCAAt      >  1:237644/1‑140 (MQ=255)                                                                                                                               aaTCCAGCGAGTCCGATCCAAGCGTCAGTGCCTCGCCGTCCAGCGTTTGCACTTCAATTTTCAGCAGGTTATGTACGGTCAGACCATATTTCAGGCAGTGGACGCCGCCGGCATTTTCAGCCACATTGCCGCCAATGGaa  >  1:1466302/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                   AGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGTTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAATCCAGCGCGTCCGATCCAAGCGTCAGTGCCTCGCCGTCCAGCGTTTGCACTTCAATTTTCAGCAGGTTATGTACGGTCAGACCATATTTCAGGCAGTGGACGCCGCCGGCATTTTCAGCCACATTGCCGCCAATGGAA  >  NC_000913/3127295‑3127560

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑