Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	309,204	C→T	P43P (CCG→CCA)	ecpC ←	putative fimbrial usher protein EcpC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	309,204	0	C	T	100.0%	226.3 / NA	66	P43P (CCG→CCA)	ecpC	putative fimbrial usher protein EcpC
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (38/28);  total (38/28)

ATGCGTAGCTGTCCATCGTCCAGCCAGATAAAAGCGCTGCCGATTCGCTGATCGTCCTGGCGACCCTGGCTACCGGCGAGATGAATATAGAGCGGGACGCTCATGCCGTCCTGAAGCGCCTGACTGAAGGCCTGCGGAATAATCACCCCACCTATTTGCTGCGCACTTATGTCAGCAGCGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTA  >  NC_000913/309070‑309340                                                                                                                                       |                                                                                                                                         aTGCGTAGCTGTCCATCGTCCAGCCAGATAAAAGCGCTGCCGATTCGCTGATCGTCCTGGCGACCCTGGCTACCGGCGAGATGAATATAGAGCGGGACGCTCATGCCGTCCTGAAGCGCCTGACTGAAGGCCTGTGGaat                                                                                                                                     >  2:821487/1‑140 (MQ=255) aTGCGTAGCTGTCCATCGTCCAGCCAGATAAAAGCGCTGCCGATTCGCTGATCGTCCTGGCGACCCTGGCTACCGGCGAGATGAATATAGAGCGGGACGCTCATGCCGTCCTGAAGCGCCTGACTGAAGGCCTGTGGaat                                                                                                                                     >  1:713419/1‑140 (MQ=255) aTGCGTAGCTGTCCATCGTCCAGCCAGATAAAAGCGCTGCCGATTCGCTGATCGTCCTGGCGACCCTGGCTACCGGCGAGATGAATATAGAGCGGGACGCTCATGCCGTCCTGAAGCGCCTGACTGAAGGCCTGTGGaat                                                                                                                                     >  2:420050/1‑140 (MQ=255)    cGTAGCTGTCCATCGTCCAGCCAGATAAAAGCGCTGCCGATTCGCTGATCGTCCTGGCGACCCTGGCTACCGGCGAGATGAATATAGAGCGGGACGCTCATGCCGTCCTGAAGCGCCTGACTGAAGGCCTGTGGaataat                                                                                                                                  <  1:767326/140‑1 (MQ=255)         cTGTCCATCGTCCAGCCAGATAAAAGCGCTGCCGATTCGCTGATCGTCCTGGCGACCCTGGCTACCGGCGAGATGAATATAGAGCGGGACGCTCATGCCGTCCTGAAGCGCCTGACTGAAGGCCTGTGGAATAATCAccc                                                                                                                             <  1:571763/140‑1 (MQ=255)         cTGTCCATCGTCCAGCCAGATAAAAGCGCTGCCGATTCGCTGATCGTCCTGGCGACCCTGGCTACCGGCGAGATGAATATAGAGCGGGACGCTCATGCCGTCCTGAAGCGCCTGACTGAAGGCCTGTGGAATAATCAccc                                                                                                                             <  2:403875/140‑1 (MQ=255)              cATCGTCCAGCCAGATAAAAGCGCTGCCGATTCGCTGATCGTCCTGGCGACCCTGGCTACCGGCGAGATGAATATAGAGCGGGACGCTCATGCCGTCCTGAAGCGCCTGACTGAAGGCCTGTGGAATAATCACCCCACCt                                                                                                                        <  2:599146/140‑1 (MQ=255)                tCGTCCAGCCAGATAAAAGCGCTACCGATTCGCTGATCGTCCTGGCGACCCTGGCTACCGGCTAGATGAATATAGAGCGGGACGCTCATGCCGCCCTGAAGCGCCTGACTGAAGGCCTGTGGAATAATCACCCCACCTAt                                                                                                                      <  2:601384/140‑1 (MQ=255)                      ggccagATAAAAGCGCTGCCGATTCGCTGATCGTCCTGGCGACCCTGGCTCCCGGCGAGATGAATATAGAGCGGGACGCTCATGCCGTCCTGAAGCGCCTGACTGAAGGCCTGTGGAATAATCACCCCACCTATTtgctg                                                                                                                <  1:1644344/139‑1 (MQ=255)                       gccagATAAAAGCGCTGCCGATTCGCTGATCGTCCTGGCGACCCTGGCTACCGGCGAGATGAATATAGAGCGGGACGCTCATGCCGTCCTGAAGCGCCTGACTGAAGGCCTGTGGAATAATCACCCCACCTATTtgctgc                                                                                                               <  2:713419/140‑1 (MQ=255)                       gccagATAAAAGCGCTGCCGATTCGCTGATCGTCCTGGCGACCCTGGCTACCGGCGAGATGAATATAGAGCGGGACGCTCATGCCGTCCTGAAGCGCCTGACTGAAGGCCTGTGGAATAATCACCCCACCTATTtgctgc                                                                                                               <  1:726022/140‑1 (MQ=255)                        ccagATAAAAGCGCTGCCGATTCGCTGATCGTCCTGGCGACCCTGGCTACCGGCGAGATGAATATAGAGCGGGACGCTCATGCCGTCCTGAAGCGCCTGACTGAAGGCCTGTGGAATAATCACCCCACCTATTTGCTgcg                                                                                                              <  1:503803/140‑1 (MQ=255)                          agATAAAAGCGCTGCCGATTCGCTGATCGTCCTGGCGACCCTGGCTACCGGCGAGATGAATATAGAGCGGGACGCTCATGCCGTCCTGAAGCGCCTGACTGAAGGCCTGTGGAATAATCACCCCACCTATTTGCTGCGCa                                                                                                            <  1:941574/140‑1 (MQ=255)                          agATAAAAGCGCTGCCGATTCGCTGATCGTCCTGGCGACCCTGGCTACCGGCGAGATGAATATAGAGCGGGACGCTCATGCCGTCCTGAAGCGCCTGACTGAAGGCCTGTGGAATAATCACCCCACCTATTTGCTGCGCa                                                                                                            <  2:1698253/140‑1 (MQ=255)                           gATAAAAGCGCTGCCGATTCGCTGATCGTCCTGGCGACCCTGGCTACCGGCGAGATGAATATAGAGCGGGACGCTCATGCCGTCCTGAAGCGCCTGACTGAAGGCCTGTGGAATAATCACCCCACCTATTTGCTGCGCAc                                                                                                           >  2:1999423/1‑140 (MQ=255)                               aaaGCGCTGCCGATTCGCTGATCGTCCTGGCGACCCTGGCTACCGGCGAGATGAATATAGAGCGGGACGCTCATGCCGTCCTGAAGCGCCTGACTGAAGGCCTGTGGAATAATCACCCCACCTATTTGCTGCGCACTTAt                                                                                                       <  1:939254/140‑1 (MQ=255)                                          gATTCGCTGATCGTCCTGGCGACCCTGGCTACCGGCGAGATGAATATAGAGCGGGACGCTCATGCCGTCCTGAAGCGCCTGACTGAAGGCCTGTGGAATAATCACCCCACCTATTTGCTGCGCACTTATGTCAGCAgcgc                                                                                            <  1:1606294/140‑1 (MQ=255)                                               gCTGATCGTCCTGGCGACCCTGGCTACCGGCGAGATGAATATAGAGCGGGACGCTCATGCCGTCCTGAAGCGCCTGACTGAAGGCCTGTGGAATAATCACCCCACCTATTTGCTGCGCACTTATGTCAGCAGCGCTGGCa                                                                                       <  1:690626/140‑1 (MQ=255)                                                cTGATCGTCCTGGCGACCCTGGCTACCGGCGAGATGAATATAGAGCGGGACGCTCATGCCGTCCTGAAGCGCCTGACTGAAGGCCTGTGGAATAATCACCCCACCTATTTGCTGCGCACTTATGTCAGCAGCGCTGGCAt                                                                                      <  1:1672413/140‑1 (MQ=255)                                                   aTCGTCCTGGCGACCCTGGCTACCGGCGAGATGAATATAGAGCGGGACGCTCATGCCGTCCTGAAGCGCCTGACTGAAGGCCTGTGGAATAATCACCCCACCTATTTGCTGCGCACTTATGTCAGCAGCGCTGGCATCgg                                                                                   >  1:769973/1‑140 (MQ=255)                                                           ggCGACCCTGGCTACCGGCGAGATGAATATAGAGCGGGACGCTCATGCCGTCCTGAAGCGCCTGACTGAAGGCCTGTGGAATAATCACCCCACCTATTTGCTGCGCACTTATGTCAGCAGCGCTGGCATCGGGCTgaacg                                                                           <  1:1249559/140‑1 (MQ=255)                                                                 ccTGGCTACCGGCGAGATGAATATAGAGCGGGACGCTCATGCCGTCCTGAAGCGCCTGACTGAAGGCCTGTGGAATAATCACCCCACCTATTTGCTGCGCACTTATGTCAGCAGCGCTGGCATCGGGCTGAACGAACaaa                                                                     >  2:1651373/1‑140 (MQ=255)                                                                        aCCGGCGAGATGAATATAGAGCGGGACGCTCATGCCGTCCTGAAGCGCCTGACTGAAGGCCTGTGGAATAATCACCCCACCTATTTGCTGCGCACTTATGTCAGCAGCGCTGGCATCGGGCTGAACGAACAAAAAGACCa                                                              >  1:1561019/1‑140 (MQ=255)                                                                          cGGCGAGATGAATATAGAGCGGGACGCTCATGCCGTCCTGAAGCGCCTGACTGAAGGCCTGTGGAATAATCACCCCACCTATTTGCTGCGCACTTATGTCAGCAGCGCTGGCATCGGGCTGAACGAACAAAAAGACCATg                                                            >  1:1891655/1‑140 (MQ=255)                                                                          cGGCGAGATGAATATAGAGCGGGACGCTCATGCCGTCCTGAAGCGCCTGACTGAAGGCCTGTGGAATAATCACCCCACCTATTTGCTGCGCACTTATGTCAGCAGCGCTGGCATCGGGCTGAACGAACAAAAAGACCATg                                                            >  2:637082/1‑140 (MQ=255)                                                                               agaTGAATATAGAGCGGGACGCTCATGCCGTCCTGAAGCGCCTGACTGAAGGCCTGTGGAATAATCACCCCACCTATTTGCTGCGCACTTATGTCAGCAGCGCTGGCATCGGGCTGAACGAACAAAAAGACCATGc                                                           >  2:136790/1‑136 (MQ=255)                                                                               agaTGAATATAGAGCGGGACGCTCATGCCGTCCTGAAGCGCCTGACTGAAGGCCTGTGGAATAATCACCCCACCTATTTGCTGCGCACTTATGTCAGCAGCGCTGGCATCGGGCTGAACGAACAAAAAGACCATGc                                                           <  1:136790/136‑1 (MQ=255)                                                                               agaTGAATATAGAGCGGGACGCTCATGCCGTCCTGAAGCGCCTGACTGAAGGCCTGTGGAATAATCACCCCACCTATTTGCTGCGCACTTATGTCAGCAGCGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGaa                                                       >  1:1110598/1‑140 (MQ=255)                                                                                   gAATATAGAGCGGGACGCTCATGCCGTCCTGAAGCGCCTGACTGAAGGCCTGTGGAATAATCACCCCACCTATTTGCTGCGCACTTATGTCAGCAGCGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCa                                                   >  2:885588/1‑140 (MQ=255)                                                                                          gagCGGGACGCTCATGCCGTCCTGAAGCGCCTGACTGAAGGCCTGTGGAATAATCACCCCACCTATTTGCTGCGCACTTATGTCAGCAGCGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTggg                                            >  1:118189/1‑140 (MQ=255)                                                                                           agCGGGACGCTCATGCCGTCCTGAAGCGCCTGACTGCAGGCCTGTGGAATAATCACCCCACCTATTTGCTGCGCACTTATGTCAGCAGCGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGc                                           >  1:21331/1‑140 (MQ=255)                                                                                           agCGGGACGCTCATGCCGTCCTGAAGCGCCTGACTGAAGGCCTGTGGAATAATCACCCCACCTATTTGCTGCGCACTTATGTCAGCAGCGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGc                                           >  2:825184/1‑140 (MQ=255)                                                                                             cGGGACGCTCATGCCGTCCTGAAGCGCCTGACTGAAGGCCTGTGGAATAATCACCCCACCTATTTGCTGCGCACTTATGTCAGCAGCGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCtt                                         >  1:686142/1‑140 (MQ=255)                                                                                              gggACGCTCATGCCGTCCTGAAGCGCCTGACTGAAGGCCTGTGGAATAATCACCCCACCTATTTGCTGTGCACTTATGTCAGCAGCGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCttt                                        <  1:1806846/140‑1 (MQ=255)                                                                                              gggACGCTCATGCCGTCCTGAAGCGCCTGACTGAAGGCCTGTGGAATAATCACCCCACCTATTTGCTGCGCACTTATGTCAGCAGCGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCttt                                        >  1:545857/1‑140 (MQ=255)                                                                                              gggACGCTCATGCCGTCCTGAAGCGCCTGACTGAAGGCCTGTGGAATAATCACCCCACCTATTTGCTGCGCACTTATGTCAGCAGCGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCttt                                        <  1:732783/140‑1 (MQ=255)                                                                                              gggACGCTCATGCCGTCCTGAAGCGCCTGACTGAAGGCCTGTGGAATAATCACCCCACCTATTTGCTGCGCACTTATGTCAGCAGCGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCttt                                        >  1:473136/1‑140 (MQ=255)                                                                                              gggACGCTCATGCCGTCCTGAAGCGCCTGACTGAAGGCCTGTGGAATAATCACCCCACCTATTTGCTGCGCACTTATGTCAGCAGCGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCttt                                        <  1:821487/140‑1 (MQ=255)                                                                                              gggACGCTCATGCCGTCCTGAAGCGCCTGACTGAAGGCCTGTGGAATAATCACCCCACCTATTTGCTGCGCACTTATGTCAGCAGCGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCttt                                        >  1:896236/1‑140 (MQ=255)                                                                                               ggACGCTCATGCCGTCCTGAAGCGCCTGACTGAAGGCCTGTGGAATAATCACCCCACCTATTTGCTGCGCACTTATGTCAGCAGCGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTc                                       >  2:760839/1‑140 (MQ=255)                                                                                                gACGCTCATGCCGTCCTGAAGCGCCTGACTGAAGGCCTGTGGAATATTCACCCCACCTATTTGCTGCGCACTTATGTCAGCAGCGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCa                                      >  1:368614/1‑140 (MQ=255)                                                                                                gACGCTCATGCCGTCCTGAAGCGCCTGACTGAAGGCCTGTGGAATAATCACCCCACCTATTTGCTGCGCACTTATGTCAGCAGCGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCa                                      >  1:666174/1‑140 (MQ=255)                                                                                                gACGCTCATGCCGTCCTGAAGCGCCTGACTGAAGGCCTGTGGAATAATCACCCCACCTATTTGCTGCGCACTTATGTCAGCAGCGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCa                                      >  1:149857/1‑140 (MQ=255)                                                                                                   gCTCATGCCGTCCTGAAGCGCCTGACTGAAGGCCTGTGGAATAATCACCCCACCTATTTGCTGCGCACTTATGTCAGCAGCGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTc                                   <  2:769973/140‑1 (MQ=255)                                                                                                   gCTCATGCCGTCCTGAAGCGCCTGACTGAAGGCCTGTGGAATAATCACCCCACCTATTTGCTGCGCACTTATGTCAGCAGCGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTc                                   <  2:1447828/140‑1 (MQ=255)                                                                                                        tGCCGTCCTGAAGCGCCTGACTGAAGGCCTGTGGAATAATCACCCCACCTATTTGCTGCGCACTTATGTCAGCAGCGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTggg                              <  1:45556/140‑1 (MQ=255)                                                                                                         gCCGTCCTGAAGCGCCTGACTGAAGGCCTGTGGAATAATCACCCCACCTATTTGCTGCGCACTTATGTCAGCAGCGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTgggg                             >  2:785920/1‑140 (MQ=255)                                                                                                             tCCTGAAGCGCCTGACTGAAGGCCTGTGGAATAATCACCCCACCTATTTGCTGCGCACTTATGTCAGCAGCGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGaa                         >  1:1498178/1‑140 (MQ=255)                                                                                                               cTGAAGCGCCTGACTGAAGGCCTGTGGAATAATCACCCCACCTATTTGCTGCGCACTTATGTCAGCAGCGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAAcc                       >  2:864967/1‑140 (MQ=255)                                                                                                                       ccTGACTGAAGGCCTGTGGAATAATCACCCCACCTATTTGCTGCGCACTTATGTCAGCAGCGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGaa                         <  1:2037754/130‑1 (MQ=255)                                                                                                                       ccTGACTGAAGGCCTGTGGAATAATCACCCCACCTATTTGCTGCGCACTTATGTCAGCAGCGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGaa                         >  2:2037754/1‑130 (MQ=255)                                                                                                                       ccTGACTGAAGGCCTGTGGAATAATCACCCCACCTATTTGCTGCGCACTTATGTCAGCAGCGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTaaa               >  2:451231/1‑140 (MQ=255)                                                                                                                       ccTGACTGAAGGCCTGTGGAATAATCACCCCACCTATTTGCTGCGCACTTATGTCAGCAGCGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTaaa               >  1:1320776/1‑140 (MQ=255)                                                                                                                       ccTGACTGAAGGCCTGTGGAATAATCACCCCACCTATTTGCTGCGCACTTATGTCAGCAGCGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTaaa               >  1:1223912/1‑140 (MQ=255)                                                                                                                       ccTGACTGAAGGCCTGTGGAATAATCACCCCACCTATTTGCTGCGCACTTATGTCAGCAGCGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTaaa               >  1:924331/1‑140 (MQ=255)                                                                                                                       ccTGACTGAAGGCCTGTGGAATAATCACCCCACCTATTTGCTGCGCACTTATGTCAGCAGCGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTaaa               >  1:1173142/1‑140 (MQ=255)                                                                                                                       ccTGACTGAAGGCCTGTGGAATAATCACCCCACCTATTTGCTGCGCACTTATGTCAGCAGCGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTaaa               >  1:84804/1‑140 (MQ=255)                                                                                                                       ccTGACTGAAGGCCTGTGGAATAATCACCCCACCTATTTGCTGCGCACTTATGTCAGCAGCGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTaaa               >  1:1131290/1‑140 (MQ=255)                                                                                                                           actgaAGGCCTGTGGAATAATCACCCCACCTATTTGCTGCGCACTTATGTCAGCAGCGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCa           <  1:873586/140‑1 (MQ=255)                                                                                                                                aGGCCTGTGGAATAATCACCCCACCTATTTGCTGCGCACTTATGTCAGCAGCGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTc      >  2:252057/1‑140 (MQ=255)                                                                                                                                aGGCCTGTGGAATAATCACCCCACCTATTTGCTGCGCACTTATGTCAGCAGCGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTc      >  2:1675191/1‑140 (MQ=255)                                                                                                                                  gCCTGTGGAATAATCACCCCACCTATTTGCTGCGCACTTATGTCAGCAGCGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGt    >  2:1733726/1‑140 (MQ=255)                                                                                                                                  gCCTGTGGAATAATCACCCCACCTATTTGCTGCGCACTTATGTCAGCAGCGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGt    >  1:2038921/1‑140 (MQ=255)                                                                                                                                    cTGTGGAATAATCACCCCACCTATTTGCTGCGCACTTATGTCAGCAGCGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTa  <  1:1636816/140‑1 (MQ=255)                                                                                                                                    cTGTGGAATAATCACCCCACCTATTTGCTGCGCACTTATGTCAGCAGCGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTa  <  2:1277441/140‑1 (MQ=255)                                                                                                                                    cTGTGGAATAATCACCCCACCTATTTGCTGCGCACTTATGTCAGCAGCGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTa  <  1:451231/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                         ATGCGTAGCTGTCCATCGTCCAGCCAGATAAAAGCGCTGCCGATTCGCTGATCGTCCTGGCGACCCTGGCTACCGGCGAGATGAATATAGAGCGGGACGCTCATGCCGTCCTGAAGCGCCTGACTGAAGGCCTGCGGAATAATCACCCCACCTATTTGCTGCGCACTTATGTCAGCAGCGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTA  >  NC_000913/309070‑309340

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑