BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	714,888	G→T	S444I (AGC→ATC)	pgm →	phosphoglucomutase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	714,888	0	G	T	97.0%	337.9 / 3.3	100	S444I (AGC→ATC)	pgm	phosphoglucomutase
Reads supporting (aligned to +/- strand): ref base G (2/1); new base T (52/45); total (54/46)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.72e-01

Only 100 of 102 total aligned reads displayed.

TTTCGACGGCACGCCGTGGTCCACCGACAAAGACGGCATCATCATGTGTCTGCTGGCGGCGGAAATCACCGCTGTCACCGGTAAGAACCCGCAGGAACACTACAACGAACTGGCAAAACGCTTTGGTGCGCCGAGCTACAACCGTTTGCAGGCAGCTGCGACTTCCGCACAAAAAGCGGCGCTGTCTAAGCTGTCTCCGGAAATGGTGAGCGCCAGCACCCTGGCAGGTGACCCGATCACCGCGCGCCTGACTGCTGCTCCGGGCAACGGT  >  NC_000913/714754‑715024

tttCGACGGCACGCCGTGGTCCACCGACAAAGACGGCATCATCATGTGTCTGCTGGCGGCGGAAATCACCGCTGTCACCGGTAAGAACCCGCAGGAACACTACAACGAACTGGCAAAACGCTTTGGTGCGCCGATCTACa                                                                                                                                     >  1:72993/1‑140 (MQ=255)

tttCGACGGCACGCCGTGGTCCACCGACAAAGACGGCATCATCATGTGTCTGCTGGCGGCGGAAATCACCGCTGTCACCGGTAAGAACCCGCAGGAACACTACAACGAACTGGCAAAACGCTTTGGTGCGCCGATCTACa                                                                                                                                     >  2:574947/1‑140 (MQ=255)

tttCGACGGCACGCCGTGGTCCACCGACAAAGACGGCATCATCATGTGTCTGCTGGCGGCGGAAATCACCGCTGTCACCGGTAAGAACCCGCAGGAACACTACAACGAACTGGCAAAACGCTTTGGTGCGCCGATCTACa                                                                                                                                     >  1:1936651/1‑140 (MQ=255)

tttCGACGGCACGCCGTGGTCCACCGACAAAGACGGCATCATCATGTGTCTGCTGGCGGCGGAAATCACCGCTGTCACCGGTAAGAACCCGCAGGAACACTACAACGAACTGGCAAAACGCTTTGGTGCGCCGATCTACa                                                                                                                                     >  2:1848875/1‑140 (MQ=255)

 ttCGACGGCACGCCGTGGTCCACCGACAAAGACGGCATCATCATGTGTCTGCTGGCGGCGGAAATCACCGCTGTCACCGGTAAGAACCCGCAGGAACACTACAACGAACTGGCAAAACGCTTTGGTGCGCCGATCTACaa                                                                                                                                    >  1:909077/1‑140 (MQ=255)

     aCGGCACGCCGTGGTCCACCGACAAAGACGGCATCATCATGTGTCTGCTGGCGGCGGAAATCACCGCTGTCACCGGTAAGAACCCGCAGGAACACTACAACGAACTGGCAAAACGCTTTGGTGCGCCGATCTACAACCGt                                                                                                                                >  1:546980/1‑140 (MQ=255)

        gCACGCCGTGGTCCACCGACAAAGACGGCATCATCATGTGTCTGCTGGCGGCGGAAATCACCGCTGTCACCGGTAAGAACCCGCAGGAACACTACAACGAACTGGCAAAACGCTTTGGTGCGCCGATCTACAACCGTTTg                                                                                                                             >  2:1073737/1‑140 (MQ=255)

          aCGCCGTGGTCCACCGACAAAGACGGCATCATCATGTGTCTGCTGGCGGCGGAAATCACCGCTGTCACCGGTAAGAACCCGCAGGAACACTACAACGAACTGGCAAAACGCTTTGGTGCGCCGATCTACAACCGTTTgca                                                                                                                           <  1:1279496/140‑1 (MQ=255)

            gCCGTGGTCCACCGACAAAGACGGCATCATCATGTGTCTGCTGGCGGCGGAAATCACCGCTGTCACCGGTAAGAACCCGCAGGAACACTACAACGAACTGGCAAAACGCTTTGGTGCGCCGATCTACAACCGTTTgcagg                                                                                                                         <  1:1172499/140‑1 (MQ=255)

              cGTGGTCCACCGACAAAGACGGCATCATCATGTGTCTGCTGGCGGCGGAAATCACCGCTGTCACCGGTAAGAACCCGCAGGAACACTACAACGAACTGGCAAAACGCTTTGGTGCGCCGATCTACAACCGTTTgcaggca                                                                                                                       <  1:1662012/140‑1 (MQ=255)

                   tCCACCGACAAAGACGGCATCATCATGTGTCTGCTGGCGGCGGAAATCACCGCTGTCACCGGTAAGAACCCGCAGGAACACTACAACGAACTGGCAAAACGCTTTGGTGCGCCGATCTACAACCGTTTGCAGGCAGCTGc                                                                                                                  >  1:909519/1‑140 (MQ=255)

                   tCCACCGACAAAGACGGCATCATCATGTGTCTGCTGGCGGCGGAAATCACCGCTGTCACCGGTAAGAACCCGCAGGAACACTACAACGAACTGGCAAAACGCTTTGGTGCGCCGATCTACAACCGTTTGCAGGCAGCTGc                                                                                                                  >  2:1880736/1‑140 (MQ=255)

                      aCCGACAAAGACGGCATCATCATGTGTCTGCTGGCGGCGGAAATCACCGCTGTCACCGGTAAGAACCCGCAGGAACACTACAACGAACTGGCAAAACGCTTTGGTGCGCCGATCTACAACCGTTTGCAGGCAGCTGCGAc                                                                                                               >  1:608361/1‑140 (MQ=255)

                        cGACAAAGACGGCATCATCATGTGTCTGCTGGCGGCGGAAATCACCGCTGTCACCGGTAAGAACCCGCAGGAACACTACAACGAACTGGCAAAACGCTTTGGTGCGCCGATCTACAACCGTTTGCAGGCAGCTGCGACtt                                                                                                             >  1:935702/1‑140 (MQ=255)

                        cGACAAAGACGGCATCATCATGTGTCTGCTGGCGGCGGAAATCACCGCTGTCACCGGTAAGAACCCGCAGGAACACTACAACGAACTGGCAAAACGCTTTGGTGCGCCGAGCTACAACCGTTTGCAGGCAGCTGCGACtt                                                                                                             >  1:1617629/1‑140 (MQ=255)

                          aCAAAGACGGCATCATCATGTGTCTGCTGGCGGCGGAAATCACCGCTGTCACCGGTAAGAACCCGCAGGAACACTACAACGAACTGGCAAAACGCTTTGGTGCGCCGATCTACAACCGTTTGCAGGCAGCTGCGACTTcc                                                                                                           >  2:1072569/1‑140 (MQ=255)

                               gACGGCATCATCATGTGTCTGCTGGCGGCGGAAATCACCGCTGTCACCGGTAAGACCCCGCAGGAACACTACAACGAACTGGCAAACCGCTTTGGTGCGCCGATCTACAACCGTTTGCAGGCAGCTGCGACTTCCGcaca                                                                                                      <  2:1667463/140‑1 (MQ=255)

                                    catcatcatGTGTCTGCTGGCGGCGGAAATCACCGCTGTCACCGGTAAGAACCCGCAGGAACACTACAACGAACTGGCAAAACGCTTTGGTGCGCCGATCTACAACCGTTTGCAGGCAGCTGCGACTTCCGCACAAAAAg                                                                                                 >  1:458257/1‑140 (MQ=255)

                                    catcatcatGTGTCTGCTGGCGGCGGAAATCACCGCTGTCACCGGTAAGAACCCGCAGGAACACTACAACGAACTGGCAAAACGCTTTGGTGCGCCGATCTACAACCGTTTGCAGGCAGCTGCGACTTCCGCACAAAAAg                                                                                                 <  2:514539/140‑1 (MQ=255)

                                       catcatGTGTCTGCTGGCGGCGGAAATCACCGCTGTCACCGGTAAGAACCCGCAGGAACACTACAACGAACTGGCAAAACGCTTTGGTGCGCCGATCTACAACCGTTTGCAGGCAGCTGCGACTTCCGCACAAAAAgcgg                                                                                              <  2:877363/140‑1 (MQ=255)

                                       catcatGTGTCTGCTGGCGGCGGAAATCACCGCTGTCACCGGTAAGAACCCGCAGGAACACTACAACGAACTGGCAAAACGCTTTGGTGCGCCGATCTACAACCGTTTGCAGGCAGCTGCGACTTCCGCACAAAAAgcgg                                                                                              <  2:1733511/140‑1 (MQ=255)

                                        atcatGTGTCTGCTGGCGGCGGAAATCACCGCTGTCACCGGTAAGAACCCGCAGGAACACTACAACGAACTGGCAAAACGCTTTGGTGCGCCGATCTACAACCGTTTGCAGGCGGCTGCGACTTCCGCACAAAAAgcggc                                                                                             <  2:1870312/140‑1 (MQ=255)

                                        atcatGTGTCTGCTGGCGGCGGAAATCACCGCTGTCACCGGTAAGAACCCGCAGGAACACTACAACGAACTGGCAAAACGCTTTGGTGCGCCGATCTACAACCGTTTGCAGGCAGCTGCGACTTCCGCACAGAAAgcggc                                                                                             <  1:1409910/140‑1 (MQ=255)

                                        atcatGTGTCTGCTGGCGGCGGAAATCACCGCTGTCACCGGTAAGAACCCGCAGGAACACTACAACGAACTGGCAAAACGCTTTGGTGCGCCGATCTACAACCGTTTGCAGGCAGCTGCGACTTCCGCACAAAAAgcggc                                                                                             <  2:558917/140‑1 (MQ=255)

                                        atcatGTGTCTGCTGGCGGCGGAAATCACCGCTGTCACCGGTAAGAACCCGCAGGAACACTACAACGAACTGGCAAAACGCTTTGGTGCGCCGATCTACAACCGTTTGCAGGCAGCTGCGACTTCCGCACAAAAAgcggc                                                                                             <  2:909077/140‑1 (MQ=255)

                                        atcatGTGTCTGCTGGCGGCGGAAATCACCGCTGTCACCGGTAAGAACCCGCAGGAACACTACAACGAACTGGCAAAACGCTTTGGTGCGCCGATCTACAACCGTTTGCAGGCAGCTGCGACTTCCGCACAAAAAgcggc                                                                                             <  1:76071/140‑1 (MQ=255)

                                        atcatGTGTCTGCTGGCGGCGGAAATCACCGCTGTCACCGGTAAGAACCCGCAGGAACACTACAACGAACTGGCAAAACGCTTTGGTGCGCCGATCTACAACCGTTTGCAGGCAGCTGCGACTTCCGCACAAAAAgcggc                                                                                             <  2:1897019/140‑1 (MQ=255)

                                        atcatGTGTCTGCTGGCGGCGGAAATCACCGCTGTCACCGGTAAGAACCCGCAGGAACACTACAACGAACTGGCAAAACGCTTTGGTGCGCCGATCTACAACCGTTTGCAGGCAGCTGCGACTTCCGCACAAAAAgcggc                                                                                             <  1:2013043/140‑1 (MQ=255)

                                        atcatGTGTCTGCTGGCGGCGGAAATCACCGCTGTCACCGGTAAGAACCCGCAGGAACACTACAACGAACTGGCAAAACGCTTTGGTGCGCCGATCTACAACCGTTTGCAGGCAGCTGCGACTTCCGCACAAAAAgcggc                                                                                             <  1:1854086/140‑1 (MQ=255)

                                        atcatGTGTCTGCTGGCGGCGGAAATCACCGCTGTCACCGGTAAGAACCCGCAGGAACACTACAACGAACTGGCAAAACGCTTTGGTGCGCCGATCTACAACCGTTTGCAGGCAGCTGCGACTTCCGCACAAAAAgcggc                                                                                             <  2:1364946/140‑1 (MQ=255)

                                        atcatGTGTCTGCTGGCGGCGGAAATCACCGCTGTCACCGGTAAGAACCCGCAGGAACACTACAACGAACTGGCAAAACGCTTTGGTGCGCCGATCTACAACCGTTTGCAGGCAGCTGCGACTTCCGCACAAAAAgcggc                                                                                             <  1:1573753/140‑1 (MQ=255)

                                         tcatGTGTCTGCTGGCGGCGGAAATCACCGCTGTCACCGGTAAGAACCCGCAGGAACACTACAACGAACTGGCAAAACGCTTTGGTGCGCCGATCTACAACCGTTTGCAGGCAGCTGCGACTTCCGCACAAAAAgtggcg                                                                                            <  2:425766/140‑1 (MQ=255)

                                         tcatGTGTCTGCTGGCGGCGGAAATCACCGCTGTCACCGGTAAGAACCCGCAGGAACACTACAACGAACTGGCAAAACGCTTTGGTGCGCCGATCTACAACCGTTTGCAGGCAGCTGCGACTTCCGCACAAAAAgcggcg                                                                                            <  2:1099538/140‑1 (MQ=255)

                                         tcatGTGTCTGCTGGCGGCGGAAATCACCGCTGTCACCGGTAAGAACCCGCAGGAACACTACAACGAACTGGCAAAACGCTTTGGTGCGCCGATCTACAACCGTTTGCAGGCAGCTGCGACTTCCGCACAAAAAgcggcg                                                                                            <  1:981844/140‑1 (MQ=255)

                                         tcatGTGTCTGCTGGCGGCGGAAATCACCGCTGTCACCGGTAAGAACCCGCAGGAACACTACAACGAACTGGCAAAACGCTTTGGTGCGCCGATCTACAACCGTTTGCAGGCAGCTGCGACTTCCGCACAAAAAgcggcg                                                                                            <  1:395943/140‑1 (MQ=255)

                                         tcatGTGTCTGCTGGCGGCGGAAATCACCGCTGTCACCGGTAAGAACCCGCAGGAACACTACAACGAACTGGCAAAACGCTTTGGTGCGCCGATCTACAACCGTTTGCAGGCAGCTGCGACTTCCGCACAAAAAgcggcg                                                                                            <  2:1162284/140‑1 (MQ=255)

                                         tcatGTGTCTGCTGGCGGCGGAAATCACCGCTGTCACCGGTAAGAACCCGCAGGAACACTACAACGAACTGGCAAAACGCTTTGGTGCGCCGATCTACAACCGTTTGCAGGCAGCTGCGACTTCCGCACAAAAAgcggcg                                                                                            <  2:72993/140‑1 (MQ=255)

                                         tcatGTGTCTGCTGGCGGCGGAAATCACCGCTGTCACCGGTAAGAACCCGCAGGAACACTACAACGAACTGGCAAAACGCTTTGGTGCGCCGATCTACAACCGTTTGCAGGCAGCTGCGACTTCCGCACAAAAAgcggcg                                                                                            <  1:472455/140‑1 (MQ=255)

                                          catGTGTCTGCTGGCGGCGGAAATCACCGCTGTCACCGGTAAGAACCCGCAGGAACACTACAACGAACTGGCAAAACGCTTTGGTGCGCCGATCTACAACCGTTTGCAGGCAGCTGCGACTTCCGCACAAAAAGCGgcgc                                                                                           <  2:956591/140‑1 (MQ=255)

                                          catGTGTCTGCTGGCGGCGGAAATCACCGCTGTCACCGGTAAGAACCCGCAGGAACACTACAACGAACTGGCAAAACGCTTTGGTGCGCCGATCTACAACCGTTTGCAGGCAGCTGCGACTTCCGCACAAAAAGCGgcgc                                                                                           <  1:471352/140‑1 (MQ=255)

                                             gtgtCTGCTGGCGGCGGAAATCACCGCTGTCACCGGTAAGAACCCGCAGGAACACTACAACGAACTGGCAAAACGCTTTGGTGCGCCGATCTACAACCGTTTGCAGGCAGCTGCGACTTCCGCACAAAAAGCGGCGCTGt                                                                                        <  2:1109849/140‑1 (MQ=255)

                                              tgtCTGCTGGCGGCGGAAATCACCGCTGTCACCGGTAAGAACCCGCAGGAACACTACAACGAACTGGCAAAACGCTTTGGTGCGCCGATCTACAACCGTTTGCAGGCAGCTGCGACTTCCGCACAAAAAGCGGCGCTGTc                                                                                       >  2:742705/1‑140 (MQ=255)

                                               gtCTGCTGGCGGCGGAAATCACCGCTGTCACCGGTAAGAACCCGCAGGAACACTACAACGAACTGGCAAAACGCTTTGGTGCGCCGATCTACAACCG‑TTGCAGGCAGCTGCGACTTCCGCACAAAAAGCGGCGCTGTCTa                                                                                     >  1:984166/1‑140 (MQ=255)

                                                tCTGCTGGCGGCGGAAATCTCCGCTGTCACCGGTAAGAACCCGCAGGAACACTACAACGAACTGGCAAAACGCTTTGGTGCGCCGATCTACAACCGTTTGCAGGCAGCTGCGACTTCCGCACAAAAAGCGGCGCTGTCTa                                                                                     >  2:562456/1‑140 (MQ=255)

                                                tCTGCTGGCGGCGGAAATCACCGCTGTCACCGGTAAGAACCCGCAGGAACACTACAACGAACTGGCAAAACGCTTTGGTGCGCCGATCTACAACCGTTTGCAGGCAGCTGCGGCTTCCGCACAAAAAGCGGCGCTGTCTa                                                                                     >  1:1595354/1‑140 (MQ=255)

                                                 ctgctgGCGGCGGAAATCACCGCTGTCACCGGTAAGAACCCGCAGGAACACTACAACGAACTGGCAAAACGCTTTGGTGCGCCGATCTACAACCGTTTGCAGGCAGCTGCGACTTCCGCACAAAAAgcggc                                                                                             >  2:1730038/1‑131 (MQ=255)

                                                 ctgctgGCGGCGGAAATCACCGCTGTCACCGGTAAGAACCCGCAGGAACACTACAACGAACTGGCAAAACGCTTTGGTGCGCCGATCTACAACCGTTTGCAGGCAGCTGCGACTTCCGCACAAAAAgcggc                                                                                             <  1:1730038/131‑1 (MQ=255)

                                                  tgctgGCGGCGGAAATCACCGCTGTCACCGGTAAGAACCCGCAGGAACACTACAACGAACTGGCAAAACGCTTTGGTGCGCCGATCTACAACCGTTTGCAGGCAGCTGCGACTTCCGCACAAAAAGCGGCGCTGTCTAAg                                                                                   <  1:1848875/140‑1 (MQ=255)

                                                  tgctgGCGGCGGAAATCACCGCTGTCACCGGTAAGAACCCGCAGGAACACTACAACGAACTGGCAAAACGCTTTGGTGCGCCGATCTACAACCGTTTGCAGGCAGCTGCGACTTCCGCACAAAAAGCGGCGCTGTCTAAg                                                                                   >  2:1864313/1‑140 (MQ=255)

                                                  tgctgGCGGCGGAAATCACCGCTGTCACCGGTAAGAACCCGCAGGAACACTACAACGAACTGGCAAAACGCTTTGGTGCGCCGATCTACAACCGTTTGCAGGCAGCTGCGACTTCCGCACAAAAAGCGGCGCTGTCTAAg                                                                                   <  2:1036788/140‑1 (MQ=255)

                                                  tgctgGCGGCGGAAATCACCGCTGTCACCGGTAAGAACCCGCAGGAACACTACAACGAACTGGCAAAACGCTTTGGTGCGCCGAGCTACAACCGTTTGCAGGCAGCTGCGACTTCCGCACAAAAAGCGGCGCTGTCTAAg                                                                                   <  1:216580/140‑1 (MQ=255)

                                                   gctgGCGGCGGAAATCACCGCTGTCACCGGTAAGAACCCGCAGGAACACTACAACGAACTGGCAAAACGCTTTGGTGCGCCGATCTACAACCGTTTGCAGGCAGCTGCGACTTCCGCACAAAAAGCGGCGCTGTCTAAGc                                                                                  >  1:1231946/1‑140 (MQ=255)

                                                    cttgcggcgGAAAACACCGCTGTCACCGGCAAGAACCCGCAGGAACACTACAACGAACTGGCAAAACGCTTTGGTGCGCCGATCTACAACCGTTTGCAGGCAGCTGCGACTTCCGCACAAAAAGCGGCGCTGTCTAAGCt                                                                                 >  2:204113/4‑140 (MQ=255)

                                                    ctgGCGGCGGAAATCACCGCTGTCACCGGTAAGAACCCGCAGGAACACTACAACGAACTGGCAAAACGCTTTGGTGCGCCGATCTACAACCGTTTGCAGGCAGCTGCGACTTCCGCACAAAAAGCGGCGCTGTCTAAGCt                                                                                 >  2:1998868/1‑140 (MQ=255)

                                                    ctgGCGGCGGAAATCACCGCTGTCACCGGTAAGAACCCGCAGGAACACTACAACGAACTGGCAAAACGCTTTGGTGCGCCGATCTACAACCGTTTGCAGGCAGCTGCGACTTCCGCACAAAAAGCGGCGCTGTCTAAGCt                                                                                 >  1:906670/1‑140 (MQ=255)

                                                    ctgGCGGCGGAAATCACCGCTGTCACCGGTAAGAACCCGCAGGAACACTACAACGAACTGGCAAAACGCTTTGGTGCGCCGATCTACAACCGTTTGCAGGCAGCTGCGACTTCCGCACAAAAAGCGGCGCTGTCTAAGCt                                                                                 >  2:16421/1‑140 (MQ=255)

                                                      ggcggcGGAAATCACCGCTGTCACCGGTAAGAACCCGCAGGAACACTACAACGAACTGGCAAAACGCTTTGGTGCGCCGATCTACAACCGTTTGCAGGCAGCTGCGACTTCCGCACAAAAAGCGGCGCTGTCTAAGCTGt                                                                               >  1:2013903/1‑140 (MQ=255)

                                                       gcggcgGAAATCACCGCTGTCACCGGTAAGAACCCGCAGGAACACTACAACGAACTGGCAAAACGCTTTGGTGCGCCGATCTACAACCGTTTGCAGGCAGCTGCGACTTCCGCACAAAAAGCGGCGCTGTCTAAGCTGtc                                                                              >  1:286661/1‑140 (MQ=255)

                                                          gcggAAATCACCGCTGTCACCGGTAAGAACCCGCAGGAACACTACAACGAACTGGCAAAACGCTTTGGTGCGCCGATCTACAACCGTTTGCAGGCAGCTGCGACTTCCGCACAAAAAGCGGCGCTGt                                                                                        >  1:2004268/1‑127 (MQ=255)

                                                          gcggAAATCACCGCTGTCACCGGTAAGAACCCGCAGGAACACTACAACGAACTGGCAAAACGCTTTGGTGCGCCGATCTACAACCGTTTGCAGGCAGCTGCGACTTCCGCACAAAAAGCGGCGCTGt                                                                                        <  2:2004268/127‑1 (MQ=255)

                                                          gcggAAATCACCGCTGTCACCGGTAAGAACCCGCAGGAACACTACAACGAACTGGCAAAACGCTTTGGTGCGCCGATCTACAACCGTTTGCAGGCAGCTGCGACTTCCGCACAAAAAGCGGCGCTGTCTAAGCTGTCTcc                                                                           >  1:1230038/1‑140 (MQ=255)

                                                          gcggAAATCACCGCTGTCACCGGTAAGAACCCGCAGGAACACTACAACGAACTGGCAAAACGCTTTGGTGCGCCGATCTACAACCGTTTGCAGGCAGCTGCGACTTCCGCACAAAAAGCGGCGCTGTCTAAGCTGTCTcc                                                                           >  1:1387109/1‑140 (MQ=255)

                                                                   aCCGCTGTCACCGGTAAGAACCCGCAGGAACACTACAACGAACTGGCAAAACGCTTTGGTGCGCCGATCTACAACCGTTTGCAGGCAGCTGCGACTTCCGCACAAAAAGCGGCGCTGTCTAAGCTGTCTCCGGAAATGGt                                                                  >  2:588645/1‑140 (MQ=255)

                                                                          tCACCGGTAAGAACCCGCAGGAACACTACAACGAACTGGCAAAACGCTTTGGTGCGCCGATCTACAACCGTTTGCAGGCAGCTGCGACTTCCGCACAAAAAGCGGCGCTGTCTAAGCTGTCTCCGGAAATGGTGAGCGcc                                                           >  2:1427751/1‑140 (MQ=255)

                                                                          tCACCGGTAAGAACCCGCAGGAACACTACAACGAACTGGCAAAACGCTTTGGTGCGCCGATCTACAACCGTTTGCAGGCAGCTGCGACTTCCGCACAAAAAGCGGCGCTGTCTAAGCTGTCTCCGGAAATGGTGAGCGcc                                                           >  2:1952942/1‑140 (MQ=255)

                                                                          tCACCGGTAAGAACCCGCAGGAACACTACAACGAACTGGCAAAACGCTTTGGTGCGCCGATCTACAACCGTTTGCAGGCAGCTGCGACTTCCGCACAAAAAGCGGCGCTGTCTAAGCTGTCTCCGGAAATGGTGAGCGcc                                                           <  1:871690/140‑1 (MQ=255)

                                                                               ggTAAGAACCCGCAGGAACACTACAACGAACTGGCAAAACGCTTTGGTGCGCCGATCTACAACCGTTTGCAGGCAGCTGCGACTTCCGCACAAAAAGCGGCGCTGTCTAAGCTGTCTCCGGAAATGGTGAGCGCCAGCAc                                                      >  2:712324/1‑140 (MQ=255)

                                                                                     aaCCCGCAGGAACACTACAACGAACTGGCAAAACGCTTTGGTGCGCCGATCTACAACCGTTTGCAGGCAGCTGCGACTTCCGCACAAAAAGCGGCGCTGTCTAAGCTGTCTCCGGAAATGGTGAGCGCCAGCACCCTGGc                                                <  2:1231946/140‑1 (MQ=255)

                                                                                       cccGCAGGAACACTACAACGAACTGGCAAAACGCTTTGGTGCGCCGATCTACAACCGTTTGCAGGCAGCTGCGACTTCCGCACAAAAAGCGGCGCTGTCTAAGCTGTCTCCGGAAATGGTGAGCGCCAGCACCCTGGCAg                                              <  1:2043434/140‑1 (MQ=255)

                                                                                        ccGCAGGAACACTACAACGAACTGGCAAAACGCTTTGGTGCGCCGATCTACAACCGTTTGCAGGCAGCTGCGACTTCCGCACAAAAAGCGGCGCTGTCTAAGCTGTCTCCGGAAATGGTGAGCGCCAGCACCCTGGCAgg                                             >  2:1871111/1‑140 (MQ=255)

                                                                                         cGCAGGAACACTACAACGAACTGGCAAAACGCTTTGGTGCGCCGATCTACAACCGTTTGCAGGCAGCTGCGACTTCCGCACAAAAAGCGGCGCTGTCTAAGCTGTCTCCGGAAATGGTGAGCGCCAGCACCCTGGCAGGt                                            >  1:457207/1‑140 (MQ=255)

                                                                                           cAGGAACACTACAACGAACTGGCAAAACGCTTTGGTGCGCCGATCTACAACCGTTTGCAGGCAGCTGCGACTTCCGCACAAAAAGCGGCGCTGTCTAAGCTGTCTCCGGAAATGGTGAGCGCCAGCACCCTGGCAGGTGa                                          >  2:1636265/1‑140 (MQ=255)

                                                                                           cAGGAACACTACAACGAACTGGCAAAACGCTTTGGTGCGCCGATCTACAACCGTTTGCAGGCAGCTGCGACTTCCGCACAAAAAGCGGCGCTGTCTAAGCTGTCTCCGGAAATGGTGAGCGCCAGCACCCTGGCAGGTGa                                          >  2:442393/1‑140 (MQ=255)

                                                                                           cAGGAACACTACAACGAACTGGCAAAACGCTTTGGTGCGCCGATCTACAACCGTTTGCAGGCAGCTGCGACTTCCGCACAAAAAGCGGCGCTGTCTAAGCTGTCTCCGGAAATGGTGAGCGCCAGCACCCTGGCAGGTGa                                          >  2:2032196/1‑140 (MQ=255)

                                                                                           cAGGAACACTACAACGAACTGGCAAAACGCTTTGGTGCGCCGATCTACAACCGTTTGCAGGCAGCTGCGACTTCCGCACAAAAAGCGGCGCTGTCTAAGCTGTCTCCGGAAATGGTGAGCGCCAGCACCCTGGCAGGTGa                                          >  2:364987/1‑140 (MQ=255)

                                                                                             ggAACACTACAACGAACTGGCAAAACGCTTTGGTGCGCCGATCTACAACCGTTTGCAGGCAGCTGCGACTTCCGCACAAAAAGCGGCGCTGTCTAAGCTGTCTCCGGAAATGGTGAGCGCCAGCACCCTGGCAGGTGAcc                                        <  1:2040562/140‑1 (MQ=255)

                                                                                                 cacTACAACGAACTGGCAAAACGCTTTGGTGCGCCGATCTACAACCGTTTGCAGGCAGCTGCGACTTCCGCACAAAAAGCGGCGCTGTCTAAGCTGTCTCCGGAAATGGTGAGCGCCAGCACCCTGGCAGGTGACCCGAt                                    >  2:556732/1‑140 (MQ=255)

                                                                                                 cacTACAACGAACTGGCAAAACGCTTTGGTGCGCCGATCTACAACCGTTTGCAGGCAGCTGCGACTTCCGCACAAAAAGCGGCGCTGTCTAAGCTGTCTCCGGAAATGGTGAGCGCCAGCACCCTGGCAGGTGACCCGAt                                    >  1:1463621/1‑140 (MQ=255)

                                                                                                    tACAACGAACTGGCAAAACGCTTTGGTGCGCCGATCTACAACCGTTTGCAGGCAGCTGCGACTTCCGCACAAAAAGCGGCGCTGTCTAAGCTGTCTCCGGAAATGGTGAGCGCCAGCACCCTGGCAGGAGACCCGATCAc                                 >  1:1441960/1‑140 (MQ=255)

                                                                                                     aCAACGAACTGGCAAAACGCTTTGGTGCGCCGATCTACAACCGTTTGCAGGCAGCTGCGACTTCCGCACAAAAAGCGGCGCTGTCTAAGCTGTCTCCGGAAATGGTGAGCGCCAGCACCCTGGCAGGTGACCCGATCAcc                                >  2:1840763/1‑140 (MQ=255)

                                                                                                        aCGAACTGGCAAAACGCTTTGGTGCGCCGATCTACAACCGTTTGCAGGCAGCTGCGACTTCCGCACAAAAAGCGGCGCTGTCTAAGCTGTCTCCGGAAATGGTGAGCGCCAGCACCCTGGCAGGTGACCCGATCACcgcg                             <  1:754799/140‑1 (MQ=255)

                                                                                                         cGAACTGGCAAAACGCTTTGGTGCGCCGATCTACAACCGTTTGCAGGCAGCTGCGACTTCCGCACAAAAAGCGGCGCTGTCTAAGCTGTCTCCGGAAATGGTGAGCGCCAGCACCCTGGCAGGTGACCCGATCACcgcgc                            >  1:41674/1‑140 (MQ=255)

                                                                                                             cTGGCAAAACGCTTTGGTGCGCCGATCTACAACCGTTTGCAGGCAGCTGCGACTTCCGCACAAAAAGCGGCGCTGTCTAAGCTGTCTCCGGAAATGGTGAGCGCCAGCACCCTGGCAGGTGACCCGATCACCGCGCGCCt                        <  1:893753/140‑1 (MQ=255)

                                                                                                                gCAAAACGCTTTGGTGCGCCGATCTACAACCGTTTGCAGGCAGCTGCGACTTCCGCACAAAAAGCGGCGCTGTCTAAGCTGTCTCCGGAAATGGTGAGCGCCAGCACCCTGGCAGGTGACCCGATCACCGCGCGCCt                        >  2:1747618/1‑137 (MQ=255)

                                                                                                                gCAAAACGCTTTGGTGCGCCGATCTACAACCGTTTGCAGGCAGCTGCGACTTCCGCACAAAAAGCGGCGCTGTCTAAGCTGTCTCCGGAAATGGTGAGCGCCAGCACCCTGGCAGGTGACCCGATCACCGCGCGCCt                        <  1:1747618/137‑1 (MQ=255)

                                                                                                                  aaaaCGCTTTGGTGCGCCGATCTACAACCGTTTGCAGGCAGCTGCGACTTCCGCACAAAAAGCGGCGCTGTCTAAGCTGTCTCCGGAAATGGTGAGCGCCAGCACCCTGGCAGGTGACCCGATCACCGCGCGCCTGActg                   <  2:1682626/140‑1 (MQ=255)

                                                                                                                    aaCGCTTTGGTGCGCCGATCTACAACCGTTTGCAGGCAGCTGCGACTTCCGCACAAAAAGCGGCGCTGTCTAAGCTGTCTCCGGAAATGGTGAGCGCCAGCACCCTGGCAGGTGACCCGATCACCGCGCGCCTGActgct                 >  1:621335/1‑140 (MQ=255)

                                                                                                                       gCTTTGGTGCGCCGATCTACAACCGTTTGCAGGCAGCTGCGACTTCCGCACAAAAAGCGGCGCTGTCTAAGCTGTCTCCGGAAATGGTGAGCGCCAGCACCCTGGCAGGTGACCCGATCACCGCGCGCCTGACTgctgct              <  1:1715428/140‑1 (MQ=255)

                                                                                                                          ttGGTGCGCCGATCTACAACCGTTTGCAGGCAGCTGCGACTTCCGCACAAAAAGCGGCGCTGTCTAAGCTGTCTCCGGAAATGGTGAGCGCCAGCACCCTGGCAGGTGACCCGATCACCGCGCGCCTGACTGCTGCTCCg           >  1:988225/1‑140 (MQ=255)

                                                                                                                          ttGGTGCGCCGATCTACAACCGTTTGCAGGCAGCTGCGACTTCCGCACAAAAAGCGGCGCTGTCTAAGCTGTCTCCGGAAATGGTGAGCGCCAGCACCCTGGCAGGTGACCCGATCACCGCGCGCCTGACTGCTGCTCCg           >  1:460898/1‑140 (MQ=255)

                                                                                                                            ggTGCGCCGATCTACAACCGTTTGCAGGCAGCTGCGACTTCCGCACAAAAAGCGGCGCTGTCTAAGCTGTCTCCGGAAATGGTGAGCGCCAGCACCCTGGCAGGTGACCCGATCACCGCGCGCCTGACTGCTGCTCCggg         <  1:742705/140‑1 (MQ=255)

                                                                                                                            ggTGCGCCGATCTACAACCGTTTGCAGGCAGCTGCGACTTCCGCACAAAAAGCGGCGCTGTCTAAGCTGTCTCCGGAAATGGTGAGCGCCAGCACCCTGGCAGGTGACCCGATCACCGCGCGCCTGACTGCTGCTCCggg         >  1:1199872/1‑140 (MQ=255)

                                                                                                                               gcgcCGATCTACAACCGTTTGCAGGCAGCTGCGACTTCCGCACAAAAAGCGGCGCTGTCTAAGCTGTCTCCGGAAATGGTGAGCGCCAGCACCCTGGCAGGTGACCCGATCACCGCGCGCCTGACTGCTGCTCCGGGCaa      <  1:1636265/140‑1 (MQ=255)

                                                                                                                                cgcCGATCTACAACCGTTTGCAGGCAGCTGCGACTTCCGCACAAAAAGCGGCGCTGTCTAAGCTGTCTCCGGAAATGGTGAGCGCCAGCACCCTGGCAGGTGACCCGATCACCGCGCGCCTGACTGCTGCTCCGGGCAAc     >  2:871953/1‑140 (MQ=255)

                                                                                                                                cgcCGATCTACAACCGTTTGCAGGCAGCTGCGACTTCCGCACAAAAAGCGGCGCTGTCTAAGCTGTCTCCGGAAATGGTGAGCGCCAGCACCCTGGCAGGTGACCCGATCACCGCGCGCCTGACTGCCGCTCCGGGCAAc     >  1:1725884/1‑140 (MQ=255)

                                                                                                                                cgcCGAGCTACAACCGTTTGCAGGCAGCTGCGACTTCCGCACAAAAAGCGGCGCTGTCTAAGCTGTCTCCGGAAATGGTGAGCGCCAGCACCCTGGCAGGTGACCCGATCACCGCGCGCCTGACTGCTGCTCCGGGCAAc     >  1:1720057/1‑140 (MQ=255)

                                                                                                                                 gcCGATCTACAACCGTTTGCAGGCAGCTGCGACTTCCGCACAAAAAGCGGCGCTGTCTAAGCTGTCTCCGGAAATGGTGAGCGCCAGCACCCTGGCAGGTGACCCGATCACCGCGCGCCTGACTGCTGCTCCGGGCAACg    >  2:316865/1‑140 (MQ=255)

                                                                                                                                   cGATCTACAACCGTTTGCAGGCAGCTGCGACTTCCGCACAAAAAGCGGCGCTGTCTAAGCTGTCTCCGGAAATGGTGAGCGCCAGCACCCTGGCAGGTGACCCGATCACCGCGCGCCTGACTGCTGCTCCGGGCAACGGt  <  2:1595354/140‑1 (MQ=255)

                                                                                                                                   cGATCTACAACCGTTTGCAGGCAGCTGCGACTTCCGCACAAAAAGCGGCGCTGTCTAAGCTGTCTCCGGAAATGGTGAGCGCCAGCACCCTGGCAGGTGACCCGATCACCGCGCGCCTGACTGCTGCTCCGGGCAACGGt  <  1:1221756/140‑1 (MQ=255)

                                                                                                                                   cGATCTACAACCGTTTGCAGGCAGCTGCGACTTCCGCACAAAAAGCGGCGCTGTCTAAGCTGTCTCCGGAAATGGTGAGCGCCAGCACCCTGGCAGGTGACCCGATCACCGCGCGCCTGACTGCTGCTCCGGGCAACGGt  <  2:909519/140‑1 (MQ=255)

TTTCGACGGCACGCCGTGGTCCACCGACAAAGACGGCATCATCATGTGTCTGCTGGCGGCGGAAATCACCGCTGTCACCGGTAAGAACCCGCAGGAACACTACAACGAACTGGCAAAACGCTTTGGTGCGCCGAGCTACAACCGTTTGCAGGCAGCTGCGACTTCCGCACAAAAAGCGGCGCTGTCTAAGCTGTCTCCGGAAATGGTGAGCGCCAGCACCCTGGCAGGTGACCCGATCACCGCGCGCCTGACTGCTGCTCCGGGCAACGGT  >  NC_000913/714754‑715024

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`