Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,142,095	G→T	A758E (GCG→GAG)	rne ←	ribonuclease E

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,142,095	0	G	T	100.0%	169.4 / NA	48	A758E (GCG→GAG)	rne	ribonuclease E
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (22/26);  total (22/26)

CGAGGCGAGCGGCGAGAACGACGCGGCATGCCACCGTTGTCACGGTTATCAGCATTGTTCTCTTCTTGCTGTTCTGGTGCAGTTTGCGCTACGACTGGCAGCGGGACTTTCACCAGTTCTGTGCGTGGAGCTGGCGCTTCCTGAACAATTGGTTCGGCAGCGACAGTTTCTTCAACCACCGGTGCGACTACCGCTTCTTCGGCTACGCTTTGCTCGTAACGCACTTTCTGATTGAGCTGACGCTGTTTACGACGCGGCTGAACCGGA  >  NC_000913/1141960‑1142226                                                                                                                                        |                                                                                                                                    cGAGGCGAGCGGCGAGAACGACGCGGCATGCCACCGTTGTCACGGTTATCAGCATTGTTCTCTTCTTGCTGTTCTGGTGCAGTTTGCGCTACGACTGGCAGCGGGACTTTCACCAGTTCTGTGCGTGGAGCTGGCTCTTc                                                                                                                                 <  2:1427140/140‑1 (MQ=255)    ggCGAGCGGCGAGAACGACGCGGCATGCCACCGTTGTCACGGTTATCAGCATTGTTCTCTTCTTGCTGTTCTGGTGCAGTTTGCGCTACGACTGGCAGCGGGACTTTCACCAGTTCTGTGCGTGGAGCTGGCTCTTCCTg                                                                                                                              <  2:185816/140‑1 (MQ=255)          cggcgAGAACGACGCGGCATGCCACCGTTGTCACGGTTATCAGCATTGTTCTCTTCTTGCTGTTCTGGTGCAGTTTGCGCTACGACTGGCAGCGGGACTTTCACCAGTTCTGTGCGTGGAGCTGGCTCTTCCTGAACAAt                                                                                                                        <  2:210932/140‑1 (MQ=255)                  acgacgCGGCATGCCACCGTTGTCACGGTTATCAGCATTGTTCTCTTCTTGCTGTTCTGGTGCAGTTTGCGCTACGACTGGCAGCGGGACTTTCACCAGTTCTGTGCGTGGAGCTGGCTCTTCCTGAACAATTGGTTCgg                                                                                                                <  2:1611459/140‑1 (MQ=255)                   cgacgCGGCATGCCACCGTTGTCACGGTTATCAGCATTGTTCTCTTCTTGCTGTTCTGGTGCAGTTTGCGCTACGACTGGCAGCGGGACTTTCACCAGTTCTGTGCGTGGAGCTGGCTCTTCCTGAACAATTGGTTCGGc                                                                                                               >  2:1093229/1‑140 (MQ=255)                    gacgCGGCATGCCACCGTTGTCACGGTTATCAGCATTGTTCTCTTCTTGCTGTTCTGGTGCAGTTTGCGCTACGACTGGCAGCGGGACTTTCACCAGTTCTGTGCGTGGAGCTGGCTCTTCCTGAACAATTGGTTCGGCa                                                                                                              <  2:885236/140‑1 (MQ=255)                           cATGCCACCGTTGTCACGGTTATCAGCATTGTTCTCTTCTTGCTGTTCTGGTGCAGTTTGCGCTACGACTGGCAGCGGGACTTTCACCAGTTCTGTGCGTGGAGCTGGCTCTTCCTGAACAATTGGTTCGGCAGCGACAg                                                                                                       >  1:200242/1‑140 (MQ=255)                            aTGCCACCGTTGTCACGGTTATCAGCATTGTTCTCTTCTTGCTGTTCTGGTGCAGTTTGCGCTACGACTGGCAGCGGGACTTTCACCAGTTCTGTGCGTGGAGCTGGCTCTTCCTGAACAATTGGTTCGGCAGCGACAGt                                                                                                      >  2:172950/1‑140 (MQ=255)                                     ttGTCACGGTTATCAGCATTGTTCTCTTCTTGCTGTTCTGGTGCAGTTTGCGCTACGACTGGCAGCGGGACTTTCACCAGTTCTGTGCGTGGAGCTGGCTCTTCCTGAACAATTGGTTCGGCAGCGACAGTTTCTTCAac                                                                                             >  1:776607/1‑140 (MQ=255)                                               tATCAGCATTGTTCTCTTCTTGCTGTTCTGGTGCAGTTTGCGCTACGACTGGCAGCGGGACTTTCACCAGTTCTGTGCGTGGAGCTGGCTCTTCCTGAACAATTGGTTCGGCAGCGACAGTTTCTTCAACCACCGGTGCg                                                                                   >  2:1396769/1‑140 (MQ=255)                                                aTCAGCATTGTTCTCTTCTTGCTGTTCTGGTGCAGTTTGCGCTACGACTGGCAGCGGGACTTTCACCAGTTCTGTGCGTGGAGCTGGCTCTTCCTGAACAATTGGTTCGGCAGCGACAGTTTCTTCAACCACCGGTGCGa                                                                                  >  1:1075028/1‑140 (MQ=255)                                                          ttCTCTTCTTGCTGTTCTGGTGCAGTTTGCGCTACGACTGGCAGCGGGACTTTCACCAGTTCTGTGCGTGGAGCTGGCTCTTCCTGAACAATTGGTTCGGCAGCGACAGTTTCTTCAACCACCGGTGCGACTACCGcttc                                                                        >  2:1435121/1‑140 (MQ=255)                                                            ctctTCTTGCTGTTCTGGTGCAGTTTGCGCTACGACTGGCAGCGGGACTTTCACCAGTTCTGTGCGTGGAGCTGGCTCTTCCTGAACAATTGGTTCGGCAGCGACAGTTTCTTCAACCACCGGTGCGACTACCGcttctt                                                                      <  2:2048017/140‑1 (MQ=255)                                                            ctctTCTTGCTGTTCTGGTGCAGTTTGCGCTACGACTGGCAGCGGGACTTTCACCAGTTCTGTGCGTGGAGCTGGCTCTTCCTGAACAATTGGTTCGGCAGCGACAGTTTCTTCAACCACCGGTGCGACTACCGcttctt                                                                      <  1:1188619/140‑1 (MQ=255)                                                            ctctTCTTGCTGTTCTGGTGCAGTTTGCGCTACGACTGGCAGCGGGACTTTCACCAGTTCTGTGCGTGGAGCTGGCTCTTCCTGAACAATTGGTTCGGCAGCGACAGTTTCTTCAACCACCGGTGCGACTACCGcttctt                                                                      <  1:1175000/140‑1 (MQ=255)                                                             tcttctTGCTGTTCTGGTGCAGTTTGCGCTACGACTGGCAGCGGGACTTTCACCAGTTCTGTGCGTGGAGCTGGCTCTTCCTGAACAATTGGTTCGGCAGCGACAGTTTCTTCAACCACCGGTGCGACTACCGCttcttc                                                                     >  1:1339196/1‑140 (MQ=255)                                                                    gCTGTTCTGGTGCAGTTTGCGCTACGACTGGCAGCGGGACTTTCACCAGTTCTGTGCGTGGAGCTGGCTCTTCCTGAACAATTGGTTCGGCAGCGACAGTTTCTTCAACCACCGGTGCGACTACCGCTTCTTCGGCTACg                                                              >  2:1080139/1‑140 (MQ=255)                                                                    gCTGTTCTGGTGCAGTTTGCGCTACGACTGGCAGCGGGACTTTCACCAGTTCTGTGCGTGGAGCTGGCTCTTCCTGAACAATTGGTTCGGCAGCGACAGTTTCTTCAACCACCGGTGCGACTACCGCTTCTTCGGCTACg                                                              >  1:1669061/1‑140 (MQ=255)                                                                         tCTGGTGCAGTTTGCGCTACGACTGGCAGCGGGACTTTCACCAGTTCTGTGCGTGGAGCTGGCTCTTCCTGAACAATTGGTTCGGCAGCGACAGTTTCTTCAACCACCGGTGCGACTACCGCTTCTTCGGCTACGCTTTg                                                         >  2:1595351/1‑140 (MQ=255)                                                                         tCTGGTGCAGTTTGCGCTACGACTGGCAGCGGGACTTTCACCAGTTCTGTGCGTGGAGCTGGCTCTTCCTGAACAATTGGTTCGGCAGCGACAGTTTCTTCAACCACCGGTGCGACTACCGCTTCTTCGGCTACGCTTTg                                                         >  1:186921/1‑140 (MQ=255)                                                                          cTGGTGCAGTTTGCGCTACGACTGGCAGCGGGACTTTCACCAGTTCTGTGCGTGGAGCTGGCTCTTCCTGAACAATTGGTTCGGCAGCGACAGTTTCTTCAACCACCGGTGCGACTACCGCTTCTTCGGCTACGCTTTGc                                                        <  2:1208635/140‑1 (MQ=255)                                                                          cTGGTGCAGTTTGCGCTACGACTGGCAGCGGGACTTTCACCAGTTCTGTGCGTGGAGCTGGCTCTTCCTGAACAATTGGTTCGGCAGCGACAGTTTCTTCAACCACCGGTGCGACTACCGCTTCTTCGGCTACGCTTTGc                                                        >  1:203068/1‑140 (MQ=255)                                                                          cTGGTGCAGTTTGCGCTACGACTGGCAGCGGGACTTTCACCAGTTCTGTGCGTGGAGCTGGCTCTTCCTGAACAATTGGTTCGGCAGCGACAGTTTCTTCAACCACCGGTGCGACTACCGCTTCTTCGGCTACGCTTTGc                                                        <  1:1620719/140‑1 (MQ=255)                                                                                  gTTTGCGCTACGACTGGCAGCGGGACTTTCACCAGTTCTGTGCGTGGAGCTGGCTCTTCCTGAACAATTGGTTCGGCAGCGACAGTTTCTTCAACCACCGGTGCGACTACCGCTTCTTCGGCTACGCTTTGCTCGTAACg                                                <  1:1080139/140‑1 (MQ=255)                                                                                  gTTTGCGCTACGACTGGCAGCGGGACTTTCACCAGTTCTGTGCGTGGAGCTGGCTCTTCCTGAACAATTGGTTCGGCAGCGACAGTTTCTTCAACCACCGGTGCGACTACCGCTTCTTCGGCTACGCTTTGCTCGTAACg                                                <  1:1226992/140‑1 (MQ=255)                                                                                  gTTTGCGCTACGACTGGCAGCGGGACTTTCACCAGTTCTGTGCGTGGAGCTGGCTCTTCCTGAACAATTGGTTCGGCAGCGACAGTTTCTTCAACCACCGGTGCGACTACCGCTTCTTCGGCTACGCTTTGCTCGTAACg                                                >  1:1990250/1‑140 (MQ=255)                                                                                  gTTTGCGCTACGACTGGCAGCGGGACTTTCACCAGTTCTGTGCGTGGAGCTGGCTCTTCCTGAACAATTGGTACGGCAGCGACAGTTTCTTCAACCACCGGTGCGACTACCGCTTCTTCGGCTACGCTTTGCTCGTAACg                                                <  2:132495/140‑1 (MQ=255)                                                                                    ttGCGCTACGACTGGCAGCGGGACTTTCACCAGTTCTGTGCGTGGAGCTGGCTCTTCCTGAACAATTGGTTCGGCAGCGACAGTTTCTTCAACCACCGGTGCGACTACCGCTTCTTCGGCTACGCTTTGCTCGTAACGCa                                              <  2:1547303/140‑1 (MQ=255)                                                                                     tGCGCTACGACTGGCAGCGGGACTTTCACCAGTTCTGTGCGTGGAGCTGGCTCTTCCTGAACAATTGGTTCGGCAGCGACAGTTTCTTCAACCACCGGTGCGACTACCGCTTCTTCGGCTACGCTTTGCTCGTAACGCAc                                             >  2:189541/1‑140 (MQ=255)                                                                                               cTGGCAGCGGGACTTTCACCAGTTCTGTGCGTGGAGCTGGCTCTTCCTGAACAATTGGTTCGGCAGCGACAGTTTCTTCAACCACCGGTGCGACTACCGCTTCTTCGGCTACGCTTTGCTCGTAACGCACTTTCTGATTg                                   >  2:689310/1‑140 (MQ=255)                                                                                                 ggCAGCGGGACTTTCACCAGTTCTGTGCGAGGCGCTGGCTCTTCCTGAACAATTGGTTCGGCAGCGACAGATTCTTCAACCACCGGTGCGACTACCGCTTCTTCGGCTACGCTTTTCTCGTAAAGCACTTTCTGATTAAg                                 >  2:1197945/1‑140 (MQ=255)                                                                                                   cAGCGGGACTTTCACCAGTTCTGTGCGTGGAGCTGGCTCTTCCTGAACAATTGGTTCGGCAGCGACAGTTTCTTCAACCACCGGTGCGACTACCGCTTCTTCGGCTACGCTTTGCTCGTAACGCACTTTCTGATTGAGCt                               <  1:89766/140‑1 (MQ=255)                                                                                                     gCGGGACTTTCACCAGTTCTGTGCGTGGAGCTGGCTCTTCCTGAACAATTGGTTCGGCAGCGACAGTTTCTTCAACCACCGGTGCGACTACCGCTTCTTCGGCTACGCTTTGCTCGTAACGCACTTTCTGATTGAGCTGa                             <  1:1412232/140‑1 (MQ=255)                                                                                                      cGGGACTTTCACCAGTTCTGTGCGTGGAGCTGGCTCTTCCTGAACAATTGGTTCGGCAGCGACAGTTTCTTCAACCACCGGTGCGACTACCGCTTCTTCGGCTACGCTTTGCTCGTAACGCACTTTCTGATTGAGCTGAc                            <  2:186921/140‑1 (MQ=255)                                                                                                      cGGGACTTTCACCAGTTCTGTGCGTGGAGCTGGCTCTTCCTGAACAATTGGTTCGGCAGCGACAGTTTCTTCAACCACCGGTGCGACTACCGCTTCTTCGGCTACGCTTTGCTCGTAACGCACTTTCTGATTGAGCTGAc                            >  2:786548/1‑140 (MQ=255)                                                                                                      cGGGACTTTCACCAGTTCTGTGCGTGGAGCTGGCTCTTCCTGAACAATTGGTTCGGCAGCGACAGTTTCTTCAACCACCGGTGCGACTACCGCTTCTTCGGCTACGCTTTGCTCGTAACGCACTTTCTGATTGAGCTGAc                            <  2:203068/140‑1 (MQ=255)                                                                                                      cGGGACTTTCACCAGTTCTGTGCGTGGAGCTGGCTCTTCCTGAACAATTGGTTCGGCAGCGACAGTTTCTTCAACCACCGGTGCGACTACCGCTTCTTCGGCTACGCTTTGCTCGTAACGCACTTTCTGATTGAGCTGAc                            >  1:327669/1‑140 (MQ=255)                                                                                                       gggACTTTCACCAGTTCTGTGCGTGGAGCTGGCTCTTCCTGAACAATTGGTTCGGCAGCGACAGTTTCTTCAACCACCGGTGCGACTACCGCTTCTTCGGCTACGCTTTGCTCGTAACGCACTTTCTGATTGAGCTGACg                           <  1:1347148/140‑1 (MQ=255)                                                                                                       gggACTTTCACCAGTTCTGTGCGTGGAGCTGGCTCTTCCTGAACAATTGGTTCGGCAGCGACAGTTTCTTCAACCACCGGTGCGACTACCGCTTCTTCGGCTACGCTTTGCTCGTAACGCACTTTCTGATTGAGCTGACg                           <  2:776607/140‑1 (MQ=255)                                                                                                        ggACTTTCACCAGTTCTGTGCGTGGAGCTGGCTCTTCCTGAACAATTGGTTCGGCAGCGACAGTTTCTTCAACCACCGGTGCGACTACCGCTTCTTCGGCTACGCTTTGCTCGTAACGCACTTTCTGATTGAGCTGACGc                          <  1:712957/140‑1 (MQ=255)                                                                                                        ggACTTTCACCAGTTCTGTGCGTGGAGCTGGCTCTTCCTGAACAATTGGTTCGGCAGCGACAGTTTCTTCAACCACCGGTGCGACTACCGCTTCTTCGGCTACGCTTTGCTCGTAACGCACTTTCTGATTGAGCTGACGc                          <  2:1137826/140‑1 (MQ=255)                                                                                                         gACTTTCACCAGTTCTGTGCGTGGAGCTGGCTCTTCCTGAACAATTGGTTCGGCAGCGACAGTTTCTTCAACCACCGGTGCGACTACCGCTTCTTCGGCTACGCTTTGCTCGTAACGCACTTTCTGATTGAGCTGACGCt                         >  2:773064/1‑140 (MQ=255)                                                                                                              tCACCAGTTCTGTGCGTGGAGCTGGCTCTTCCTGAACAATTGGTTCGGCAGCGACAGTTTCTTCAACCACCGGTGCGACTACCGCTTCTTCGGCTACGCTTTGCTCGTAACGCACTTTCTGATTGAGCTGACGCTGTTTa                    >  1:1190582/1‑140 (MQ=255)                                                                                                                 ccAGTTCTGTGCGTGGAGCTGGCTCTTCCTGAACAATTGGTTCGGCAGCGACAGTTTCTTCAACCACCGGTGCGACTACCGCTTCTTCGGCTACGCTTTGCTCGTAACGCACTTTCTGATTGAGCTGACGCTGTTTacga                 <  2:822362/140‑1 (MQ=255)                                                                                                                            cGTGGAGCTGGCTCTTCCTGAACAATTGGTTCGGCAGCGACAGTTTCTTCAACCACCGGTGCGACTACCGCTTCTTCGGCTACGCTTTGCTCGTAACGCACTTTCTGATTGAGCTGACGCTGTTTACGACGCGGCTGAAc      <  2:1565148/140‑1 (MQ=255)                                                                                                                              tGGAGCTGGCTCTTCCTGAACAATTGGTTCGGCAGCGACAGTTTCTTCAACCACCGGTGCGACTACCGCTTCTTCGGCTACGCTTTGCTCGTAACGCACTTTCTGATTGAGCTGACGCTGTTTACGACGCGGCTGAACCg    >  1:346463/1‑140 (MQ=255)                                                                                                                               ggAGCTGGCTCTTCCTGAACAATTGGTTCGGCAGCGACAGTTTCTTCAACCACCGGTGCGACTACCGCTTCTTCGGCTACGCTTTGCTCGTAACGCACTTTCTGATTGAGCTGACGCTGTTTACGACGCGGCTGAACCgg   >  2:572418/1‑140 (MQ=255)                                                                                                                               ggAGCTGGCTCTTCCTGAACAATTGGTTCGGCAGCGACAGTTTCTTCAACCACCGGTGCGACTACCGCTTCTTCGGCTACGCTTTGCTCGTAACGCACTTTCTGATTGAGCTGACGCTGTTTACGACGCGGCTGAACCgg   <  2:690154/140‑1 (MQ=255)                                                                                                                                gAGCTGGCTCTTCCTGAACAATTGGTTCGGCAGCGACAGTTTCTTCAACCACCGGTGCGACTACCGCTTCTTCGGCTACGCTTTGCTCGTAACGCACTTTCTGATTGAGCTGACGCTGTTTACGACGCGGCTGAACCGGa  <  2:1909283/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                    CGAGGCGAGCGGCGAGAACGACGCGGCATGCCACCGTTGTCACGGTTATCAGCATTGTTCTCTTCTTGCTGTTCTGGTGCAGTTTGCGCTACGACTGGCAGCGGGACTTTCACCAGTTCTGTGCGTGGAGCTGGCGCTTCCTGAACAATTGGTTCGGCAGCGACAGTTTCTTCAACCACCGGTGCGACTACCGCTTCTTCGGCTACGCTTTGCTCGTAACGCACTTTCTGATTGAGCTGACGCTGTTTACGACGCGGCTGAACCGGA  >  NC_000913/1141960‑1142226

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑