Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,429,9840TG36.8% 50.7 / 23.2 38L41V (TTG→GTG) cycAserine/alanine/glycine/cycloserine:H(+)symporter
Reads supporting (aligned to +/- strand):  ref base T (3/21);  new base G (14/0);  total (17/21)
Fisher's exact test for biased strand distribution p-value = 7.03e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

TACAGGAAGAAAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATGCGGGCAATGGGGGAATTGCTGCTTTCGAATCTGGAATACAAATC  >  NC_000913/4429847‑4430120
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tACAGGAAGAAAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGGCATTGGTACGGGgttg                                                                                                                                        >  1:465755/1‑140 (MQ=255)
tACAGGAAGAAAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGgt                                                                                                                                          <  1:223538/138‑1 (MQ=255)
tACAGGAAGAAAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGgt                                                                                                                                          >  2:223538/1‑138 (MQ=255)
          aaaaaaCATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTGTTTATGGGGt                                                                                                                              <  1:542241/140‑1 (MQ=255)
             aaaCATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCCTTGGTACGGGGGTGTTTTTGGGGTCTg                                                                                                                           >  2:32978/1‑140 (MQ=255)
               aCATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGGTGTTTTTGGGGTCTGGc                                                                                                                         >  2:474261/1‑140 (MQ=255)
                   ggTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTTGGACGGGGCTGATTATGGGGTCTGGCaaaa                                                                                                                     >  2:558031/1‑140 (MQ=255)
                        aTCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTGTTTATGGGGTCTGGCaa                                                                                                                       <  2:272147/133‑1 (MQ=255)
                        aTCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGGTGTTTATGGGGTCTGGCaa                                                                                                                       >  1:272147/1‑133 (MQ=255)
                          cAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAg                                                                                                              <  2:295175/140‑1 (MQ=255)
                             gTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGGTGTTTATGGGGTCTGGCAAAACGATTAGCCt                                                                                                           >  1:468157/1‑140 (MQ=255)
                                    tCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCggg                                                                                                    <  1:430807/140‑1 (MQ=255)
                                           cGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGa                                                                                             <  1:315848/140‑1 (MQ=255)
                                                atCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCAtt                                                                                        <  1:429754/140‑1 (MQ=255)
                                                 tCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCACTGGCGGTGCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCAttt                                                                                       <  1:45316/140‑1 (MQ=255)
                                                    ggCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCg                                                                                    <  2:758425/140‑1 (MQ=255)
                                                    ggCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCg                                                                                    <  1:378706/140‑1 (MQ=255)
                                                           gCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGGACGGGGGTGTTTTTTGGGTCTTGCAAAAAGATTAGCCTTGCCGGGGCGTCGATCCTTTTTGTTTttat                                                                             >  2:240067/1‑140 (MQ=255)
                                                           gCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGGACGGGGGTGTTTATTGGGTCTGGCAAAAAGATTAGCCTTTCCGGGCCGTCGATCATTTTTGTTTatat                                                                             >  1:397203/1‑140 (MQ=255)
                                                               aaCAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGGTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATc                                                                         >  1:409696/1‑140 (MQ=255)
                                                                 cAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCCTTGGTACGGGGGTGTTTTTGGGGGCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCAt                                                                       >  2:461851/1‑140 (MQ=255)
                                                                    tCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGGACGGGGTTGTTTTTGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTgg                                                                    >  1:350622/1‑140 (MQ=255)
                                                                        tACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGtttt                                                                <  2:409696/140‑1 (MQ=255)
                                                                          cGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTAt                                                              <  2:587594/140‑1 (MQ=255)
                                                                                          aCCGACATATTCAGCTTATTGCCATTGGCGGTGCCCTTGGTACCGGGGTGTTTATGGGGGCTTGCAAAACGATTTTGCTTTCCGGGCCGTCGATCATTTTCGGTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATg                                              >  2:73698/1‑140 (MQ=255)
                                                                                          aCCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATg                                              <  2:468157/140‑1 (MQ=255)
                                                                                          aCCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATg                                              <  2:465755/140‑1 (MQ=255)
                                                                                           ccGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTAC‑GGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATGCg                                            <  2:557927/140‑1 (MQ=255)
                                                                                            cGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATGCg                                            <  1:474261/140‑1 (MQ=255)
                                                                                            cGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATGCg                                            <  1:48709/140‑1 (MQ=255)
                                                                                            cGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGGTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATGCg                                            >  1:482464/1‑140 (MQ=255)
                                                                                            cGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGGACGGGGGTGTTTATGGGGGCTGGCAAAACGATTAGCCTTTCCGGGCCGTCGGTCATTTTCGTTTATATTATCATTGGTTTTTTGCTCTTTTTCGTGATGCg                                            >  2:585234/1‑140 (MQ=255)
                                                                                                  atTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATGCGGGCAAt                                      <  2:287681/140‑1 (MQ=255)
                                                                                                  atTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATGCGGGCAAt                                      <  2:11050/140‑1 (MQ=255)
                                                                                                        cTTATTGCCATTGGCGGTGCCATTTGTACGGGGGTGTTTTTGGGGGCTGGCAAAACGAATAGCCTTGCCGGGGCGTCGATCATTTTTGTTTATATGATCATTGGTTTTTTGCTCTTTTTTGTGATGCGGGCAATgggggg                                >  2:594550/1‑139 (MQ=255)
                                                                                                         ttATTGCCATTGGCGGTGCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATGCGGGCAATGGGGGaa                               <  1:415014/140‑1 (MQ=255)
                                                                                                           aTTGCCATTGGCGGTGCCATTGGTTCGGGGGTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATGCGGGCAATGGGGGAAtt                             >  1:329644/1‑140 (MQ=255)
                                                                                                           aTTGCCATTGGCGGTGCCATTGGTACGGGGGTGTTTTTGGGGGCTGGCAAAACGATTAGCCCTCCCCGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTTTGCTCTTTTTTGTGATGCGGGCAATGGGGGGAtt                             >  2:253022/1‑140 (MQ=255)
                                                                                                                  ttGGCGGTGCCATTGGTACGGGGGTGTTTTTGGGGGCTGGCAAAACGATTAGCCTTTCCGGGCCATCGATCATTTTTGTTTATATGATCATTTGTTTTTTGCTCTTTTTTGTGATGCGGGCAATGGGGGGAATGCTGCtt                      >  2:758228/1‑140 (MQ=255)
                                                                                                                  ttGGCGGTGCCATTGGTACGGGGGTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATGCGGGCAATGGGGGAATTGCTGCtt                      >  2:223777/1‑140 (MQ=255)
                                                                                                                         tGCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATGCGGGCAATGGGGGAATTGCTGCTTTCGAATc               <  1:253022/140‑1 (MQ=255)
                                                                                                                                     ggggTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATGCGGGCAATGGGGGAATTGCTGCTTTCGAATCTGGAATACaa     <  2:302118/138‑1 (MQ=255)
                                                                                                                                     ggggTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATGCGGGCAATGGGGGAATTGCTGCTTTCGAATCTGGAATACaa     >  1:302118/1‑138 (MQ=255)
                                                                                                                                      gggTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATGCGGGCAATGGGGGAATTGCTGCTTTCGAATCTGGAATACAAATc  >  2:22087/1‑140 (MQ=255)
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TACAGGAAGAAAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATGCGGGCAATGGGGGAATTGCTGCTTTCGAATCTGGAATACAAATC  >  NC_000913/4429847‑4430120

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑