Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,343,256	C→A	G305V (GGA→GTA)	yfaL ←	putative autotransporter adhesin YfaL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,343,256	0	C	A	100.0%	113.2 / NA	34	G305V (GGA→GTA)	yfaL	putative autotransporter adhesin YfaL
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (26/8);  total (26/8)

ATCTCACCAGTAAAGTCATTGTTATCTGCATTAAGTACCAGATCGCCGGAACCTGTTTTGGTGATTAACCCGGTACCAGCAATAGAGTCAACAGCTCCGTCATTC‑TCTGTATTGCCAATAACCAGCGTTTTTCCGTCGGCAATATCAAAGGTAACTTCACTTAAGCCGAGATACATAAAGCCACCCGCCGCAGAGGAAGGACCATCTCCATAGCCTGCTGCGCTATTGTTCTCATCGACTAACACGCCTCCGTTCTGGCTGTAGC  >  NC_000913/2343124‑2343388                                                                                                                                      |                                                                                                                                     aTCTCACCAGTAAAGTCATTGTTATCTGCATTAAGTACCAGATCGCCGGAACCTGTTTTGGTGATTAACCCGGTACCAGCAATAGAGTCAACAGCTCCGTCATTC‑TCTGTATTGCCAATAACCAGCGTTTTTACGTCGGc                                                                                                                               <  2:621447/140‑1 (MQ=255)       ccAGTAAAGTCATTGTTATCTGCATTAAGTACCAGATCGCCGGAACCTGTTTTGGTGATTAACCCGGTACCAGCAATAGAGTCAACAGCTCCGTCATTC‑TCTGTATTGCCAATAACCAGCGTTTTTACGTCGGCAATATc                                                                                                                         <  2:313988/140‑1 (MQ=255)                  aTTGTTATCTGCATTAAGTACCAGATCGCCGGAACCTGTTTTGGTGATTAACCCGGTACCAGCAATAGAGTCAACAGCTCCGTCATTC‑TCTGTATTGCCAATAACCAGCGTTTTTACGTCGGCAATATCAAAGGTAACtt                                                                                                              <  2:197585/140‑1 (MQ=255)                        aTCTGCATTAAGTACCAGATCGCCGGAACCTGTTTTGGTGATTAACCCGGTACCAGCAATAGAGTCAACAGCTCCGTCATTC‑TCTGTATTGCCAATAACCAGCGTTTTTACGTCGGCAATATCAAAGGTAACTTCACTTa                                                                                                        >  2:560871/1‑140 (MQ=255)                        aTCTGCATTAAGTACCAGATCGCCGGAACCTGTTTTGGTGATTAACCCGGTACCAGCAATAGAGTCAACAGCTCCGTCATTC‑TCTGTATTGCCAATAACCAGCGTTTTTACGTCGGCAATATCAAAGGTAACTTCACTTa                                                                                                        >  1:759701/1‑140 (MQ=255)                                tAAGTACCAGATCGCCGGAACCTGTTTTGGTGATTAACCCGGTACCAGCAATAGAGTCAACAGCTCCGTCATTC‑TCTGTATTGCCAATAACCAGCGTTTTTACGTCGGCAATATCAAAGGTAACTTCACTTAAGCCgaga                                                                                                >  2:614198/1‑140 (MQ=255)                                tAAGTACCAGATCGCCGGAACCTGTTTTGGTGATTAACCCGGTACCAGCAATAGAGTCAACAGCTCCGTCATTC‑TCTGTATTGCCAATAACCAGCGTTTTTACGTCGGCAATATCAAAGGTAACTTCACTTAAGCCgaga                                                                                                <  1:678094/140‑1 (MQ=255)                                       cAGATCGCCGGAACCTGTTTTGGTGATTAACCCGGTACCAGCAATAGAGTCAACAGCTCCGTCATTC‑TCTGTATTGCCAATAACCAGCGTTTTTACGTCGGCAATATCAAAGGTAACTTCACtt                                                                                                         <  2:285161/124‑1 (MQ=255)                                       cAGATCGCCGGAACCTGTTTTGGTGATTAACCCGGTACCAGCAATAGAGTCAACAGCTCCGTCATTC‑TCTGTATTGCCAATAACCAGCGTTTTTACGTCGGCAATATCAAAGGTAACTTCACtt                                                                                                         >  1:285161/1‑124 (MQ=255)                                          aTCGCCGGAACCTGTTTTGGTGATTAACCCGGTACCAGCAATAGAGTCAACAGCTCCGTCATTC‑TCTGTATTGCCAATAACCAGCGTTTTTACGTCGGCAATATCAAAGGTAACTTCACTTAAGCCGAGATACATAAAGc                                                                                      >  1:757291/1‑140 (MQ=255)                                            cGCCGGAACCTGTTTTGGTGATTAACCCGGTACCAGCAATAGAGTCAACAGCTCCGTCATTC‑TCTGTATTGCCAATAACCAGCGTTTTTACGTCGGCAATATCAAAGGTAACTTCACTTAAGCCGAGATACATAAAGCCa                                                                                    >  1:614456/1‑140 (MQ=255)                                            cGCCGGAACCTGTTTTGGTGATTAACCCGGTACCAGCAATAGAGTCAACAGCTCCGTCATTC‑TCTGTATTGCCAATAACCAGCGTTTTTACGTCGGCAATATCAAAGGTAACTTCACTTAAGCCGAGATACATAAAGCCa                                                                                    >  1:648004/1‑140 (MQ=255)                                            cGCCGGAACCTGTTTTGGGGATTAACCCGGTACCAGCAATAGAGTCAACAGCTCCGTCATTC‑TCTGTATTGCCAATAACCAGCGTTTTTACGTCGGCAATATCAAAGGTAACTTCACTTAAGCCGAGATACATAAAGCCa                                                                                    >  1:269500/1‑140 (MQ=255)                                              ccGGACCTTTTTTTGGTGATTACCCCGGTACCAGAAATAGAGTCAACAGCCCCGTCATTC‑TCTGTATTGCCAAAAACCAGCGTTTTTCCGTCGGAAATACCAAAGGTAACTTCACTAAAGCCGAGATACAAAAAcccccc                                                                                  <  2:680915/140‑1 (MQ=255)                                              ccGGAACCTGTTTTGGTGATTACCCCGGTACCAGAAAAAGAGTCAACAGCTCCTTCATTC‑TCTGTATTGCAAATAACCAGCTTTTTTACGTCGGCAATATCAAAGGTAACTTCACTTAAGCCGAGATACATAAAGCCAcc                                                                                  <  2:759701/140‑1 (MQ=255)                                                          ttGGTGATTACCCCGGTACCAGCAATAGAGTCAACAGCTCCGTCATTC‑TCTGTATTGCCAATACCCAGCGTTTTTACGTCGGCAATATCAAAGGTACCTTCACTTAAGCCGAGATACATAAAGCCACCCGCCGCAGagga                                                                      <  2:472567/140‑1 (MQ=255)                                                            ggTGATTAACCCGGTACCAGCAATAGAGTCAACAGCTCCGTCATTC‑TCTGTATTGCCAATAACCAGCGTTTTTACGTCGGCAATATCAAAGGTAACTTCACTTAAGCCGAGATACATAAAGCCACCCGCCGCAGaggaag                                                                    >  1:154538/1‑140 (MQ=255)                                                             gTGATTACCCCGGTACCAGCAATAGAGTCAACAGCTCCGTCATTC‑TCTGTATTGCCAATACCCAGCGTTTTTACGTCGGCAATATCAAAGGTAACTTCACTTAAGCCGAGATACATAAAGCCACCCGCCGCAGaggaagg                                                                   <  1:529478/140‑1 (MQ=255)                                                                  tAACCCGGTACCAGAAATAGAGTCAACACCTCCGTCTTTC‑TTTTTATTGCCAATACCCAGCTTTTTTCCGCCGGCAATATCAAAGGTACCTTCACTTAAGCCGAGATACAAAAACCCCCCCGCCGCAGAGGAAGGACCAt                                                              <  2:235650/140‑1 (MQ=255)                                                                          tACCAGCAATAGAGTCAACAGCTCCGTCATTC‑TCTGTATTGCCAATAACCAGCGTTTTTACGTCGGCAATATCAAAGGTAACTTCACTTAAGCCGAGATACATAAAGCCACCCGCCGCAGAGGAAGGACCATCTCCATAg                                                      >  1:362304/1‑140 (MQ=255)                                                                          tACCAGCAATAGAGTCAACAGCTCCGTCATTC‑TCTGTATTGCCAATAACCAGCGTTTTTACGTCGGCAATATCAAAGGTAACTTCACTTAAGCCGAGATACATAAAGCCACCCGCCGCAGAGGAAGGACCATCTCCATAg                                                      >  1:172212/1‑140 (MQ=255)                                                                          tACCAGCAATAGAGTCAACAGCTCCGTCATTC‑TCTGTATTGCCAATAACCAGCGTTTTTACGTCGGCAACATCAAAGGTAACTTCACTTAAGCCGAGATACATAAAGCCACCCGCCGCAGAGGAAGGACCATCTCCATAg                                                      >  1:622446/1‑140 (MQ=255)                                                                              aGCAATAGAGTCAACAGCTCCGTCATTC‑TCTGTATTGCCAATAACCAGCGTTTTTACGTCGGCAATATCAAAGGTAACTTCACTTAAGCCGAGATACATAAAGCCACCCGCCGCAGAGGAAGGACCATCTCCATAGCctg                                                  >  1:481169/1‑140 (MQ=255)                                                                                 aaTAGAGTCAACAGCTCCGTCATTC‑TCTGTTTTGCAAATAACCAGCTTTTTTACGTCGGAAATATCAAAGGTAACTTCACTTAAGCCGAGATACAAAAAGCCCCCCGCCGCAGAGGAAGGACCATCTCCATAGCctgctg                                               <  2:479370/140‑1 (MQ=255)                                                                                 aaTAGAGACAACGGCT‑CGTTATTCTTCTGTATTCCCAATAACCAGCTTTTTCACGTCGCAAAGATAAAAGTTATCTTCTTTTAAGCCGAGATATAGAACCCCACCCGCCGCAGAGGAAGGACCATCTCCATAGCctgctg                                               <  2:471545/140‑1 (MQ=255)                                                                                   tAGAGTCAACAGCTCCGTCATTC‑TCTGTATTGCCAATAACCAGCGTTTTTACGTCGGCAATATCAAAGGTAACTTCACTTAAGCCGAGATACATAAAGCCACCCGCCGCAGAGGAAGGACCATCTCCATAGCCTGCTgcg                                             >  1:479370/1‑140 (MQ=255)                                                                                   tAGAGTCAACAGCTCCGTCATTC‑TCTGTATTGCCAATAACCAGCGTTTTTACGTCGGCAATATCAAAGGTAACTTCACTTAAGCCGAGATACATAAAGCCACCCGCCGCAGAGGAAGGACCATCTCCATAGCCTGCTgcg                                             >  1:338140/1‑140 (MQ=255)                                                                                      agTCACCAGCTCCGTCATTC‑TTTTTTTTAACAAAAACCAGCGTTTTCACGTCGACAATAAAAAAGGTACCTTCACTTACCCCGAGAAACAAAAAGCCCCCCGCCGCAGAGGAAGGACCATCTCCATAGCCTGCTGCGCTa                                          <  2:690244/140‑1 (MQ=255)                                                                                        tCAACAGCTCCGTCATTC‑TCTGTATTGCCAATAACCAGCGTTTTTACGTCGGCAATATCAAAGGTAACTTCACTTAAGCCGAGATACATAAAGCCACCCGCCGCAGAGGAAGGACCATCTCCATAGCCTGCTGCGCTAtt                                        >  2:777282/1‑140 (MQ=255)                                                                                          aaCAGCTCCGTCATTC‑TCTGTATTGCCAATAACCAGCGTTTTTACGTCGGCAATATCAAAGGTAACTTCACTTAAGCCGAGATACATAAAGCCACCCGCCGCAGAGGAAGGACCATCTCCATAGCCTGCTGCGCTATTGt                                      >  1:681654/1‑140 (MQ=255)                                                                                             aGCTCCTTCATTC‑TCTGTATTGCCAATAACCAGTTTTTTTACGTCGGCAATATAAAAGGTAACTTCACTTAAGCCGAGATACAAAAAGCCACCCGCCGCAGAGGAAGGACCATCTCCATAGCCTGCTGCGCTATTGTtct                                   <  2:113017/140‑1 (MQ=255)                                                                                             aGCTCCGTCATTC‑TCTGTATTGCCAATAACCAGCGTTTTTACGTCGGCAATATCAAAGGTAACTTCACTTAAGCCGAGATACATAAAGCCACCCGCCGCAGAGGAAGGACCATCTCCATAGCCTGCTGCGCTATTGTtct                                   >  1:85218/1‑140 (MQ=255)                                                                                               cTCCGTCATTC‑TCTGTATTGCCAATAACCAGCGTTTTTACGTCGGCAATATCAAAGGTAACTTCACTTAAGCCGAGATACATAAAGCCACCCGCCGCAGAGGAAGGACCATCTCCATAGCCTGCTGCGCTATTGTTCTCa                                 >  1:113017/1‑140 (MQ=255)                                                                                                  cGTCATTC‑TCTGTATTGCCAATAACCAGCGTTTTTACGTCGGCAATATCAAAGGTAACTTCACTTAAGCCGAGATACATAAAGCCACCCGCCGCAGAGGAAGGACCATCTCCATAGCCTGCTGCGCTATTGTTCTCATCg                              >  2:280442/1‑140 (MQ=255)                                                                                                  cGTCATTC‑TCTGTATTGCCAATAACCAGCGTTTTTACGTCGGCAATATCAAAGGTAACTTCACTTAAGCCGAGATACATAAAGCCACCCGCCGCAGAGGAAGGACCATCTCCATAGCCTGCTGCGCTATTGTTCTCATCg                              >  1:330970/1‑140 (MQ=255)                                                                                                    tctttC‑TCTGTATTGCAAATACCCAGCTTTTTTCCGTCGGCAATATCAAAGGTAACTTCACTAAAGCCGAGATACATAAACCCACCCGCCGCAGAGGAAGGACCATCTCCATAGCCTGCTGCGCTATTGTTCTCATCGAc                            <  2:648004/137‑1 (MQ=255)                                                                                                          gcttttATTCCAAATAACCTGCTTTTTTACGCCGAAAAAATAAAAGATACCTCGAGTTAAGCATAAAACTAAAAACCCAGCCGCCGCAGAGGAAGGACCATCTCCATAGCCTGCTGCGCTATTGTTCTCATCGACTAaca                       <  2:79782/135‑1 (MQ=255)                                                                                                             tGTATTGCCAATAACCAGCGTTTTTACGTCGGCAATATCAAAGGTAACTTCACTTAAGCCGAGATACATAAAGCCACCCGCCGCAGAGGAAGGACCATCTCCATAGCCTGCTGCGCTATTGTTCTCATCGACTAACACGc                    >  2:216945/1‑140 (MQ=255)                                                                                                                    ccAATAACCAGCTTTTTTACGTCGGCAATATCAAGGGTAACTTCTCTAAAGCCGAGATACAAAAAGCCACCCGCCGCAGAGGAAGGACCATCTCCATAGCCTGCTGCGCTATTGTTCTCATCGACTAACACGCCTCCGtt             <  1:560871/140‑1 (MQ=255)                                                                                                                    ccAATAACCAGCGTTTTTACGTCGGCAATATCAAAGGTAACTTCACTTAAGCCGAGATACATAAAGCCACCCGCCGCAGAGGAAGGACCATCTCCATAGCCTGCTGCGCTATTGTTCTCATCGACTAACACGCCTCCGtt             >  1:410884/1‑140 (MQ=255)                                                                                                                    caaaTACCCAGCGTTTTTACGTCGGCAATATAAAAGGTACCTTCACTAAAGCCGAGATACAAAAAGCCCCCCGCCGCAGAGGAAGGACCATCTCCATAGCCTGCTGCGCTATTGTTCTCATCGACTAACACGACTCCGtt             <  2:481169/138‑1 (MQ=255)                                                                                                                       aTAACCAGCGTTTTTACGTCGGCAATATCAAAGGTAACTTCACTTAAGCCGAGATACATAAAGCCACCCGCCGCAGAGGAAGGACCATCTCCATAGCCTGCTGCGCTATTGTTCTCATCGACTAACACGCCTCCGTTcct          >  1:79782/1‑138 (MQ=255)                                                                                                                           ccAGCTTTTTTACGTCGGAAATAAAAAAGGTAACTTCCCTTAAGCCGAGATAAATAAACCCCCCCGCCGCAGAGGAAGGACCATCTCCATAGCCTGCTGCGCTATTGTTCTCATCGACTAACACGCCTCCGTTCTGGCTg      <  2:338140/140‑1 (MQ=255)                                                                                                                              gCGTTTTTACGTCGGCAATATCAAAGGTAACTTCACTTAAGCCGAGATACATAAAGCCACCCGCCGCAGAGGAAGGACCATCTCCATAGCCTGCTGCGCTATTGTTCTCATCGACTAACACGCCTCCGTTCTGGCTGTAg   >  2:48723/1‑140 (MQ=255)                                                                                                                               cGTTTTTACGTCGGCAATATCAAAGGTAACTTCACTTAAGCCGAGATACATAAAGCCACCCGCCGCAGAGGAAGGACCATCTCCATAGCCTGCTGCGCTATTGTTCTCATCGACTAACACGCCTCCGTTCTGGCTGTAGc  >  1:144868/1‑140 (MQ=255)                                                                                                                                      |                                                                                                                                     ATCTCACCAGTAAAGTCATTGTTATCTGCATTAAGTACCAGATCGCCGGAACCTGTTTTGGTGATTAACCCGGTACCAGCAATAGAGTCAACAGCTCCGTCATTC‑TCTGTATTGCCAATAACCAGCGTTTTTCCGTCGGCAATATCAAAGGTAACTTCACTTAAGCCGAGATACATAAAGCCACCCGCCGCAGAGGAAGGACCATCTCCATAGCCTGCTGCGCTATTGTTCTCATCGACTAACACGCCTCCGTTCTGGCTGTAGC  >  NC_000913/2343124‑2343388

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑