Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	736,223	C→A	intergenic (+4/‑222)	ybfC → / → ybfQ	uncharacterized protein YbfC/inactive transposase YbfQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	736,223	0	C	A	100.0%	220.7 / NA	62	intergenic (+4/‑222)	ybfC/ybfQ	uncharacterized protein YbfC/inactive transposase YbfQ
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (36/26);  total (36/26)

CAATCATGGTAAAATATTTTAAAGATGAGAAACCAACTTATGCTCGAATGACAGCACATCTCCCGAACAAAAATGAGTCTTACGAATGTTTAATAAAGATACAGCATGAACTAATTCGTAGTGAAGAGAAATAAAATCTCTTCCTGAGAAAACAAATTAATCATTGTGAAAAATTATATAACTCATGGAATAGGTGTTTATTTTGCGTTCCATTTGCAGGGAAAGATCACGTAACGCTACTTTTTTGTACTAAATAATTCGAATTTTAAGTTC  >  NC_000913/736086‑736358                                                                                                                                          |                                                                                                                                        cAATCATGGTAAAATATTTTAAAGATGAGAAACCAACTTATGCTCGAATGACAGCACATCTCCCGAACAAAAATGAGTCTTACGAATGTTTAATAAAGATACAGCATGAACTAATTCGTAGTGAAGAGAAATAAAAtatc                                                                                                                                       >  2:671096/1‑140 (MQ=255)    tCATGGTAAAATATTTTAAAGATGAGAAACCAACTTATGCTCGAATGACAGCACATCTCCCGAACAAAAATGAGTCTTACGAATGTTTAATAAAGATACAGCATGAACTAATTCGTAGTGAAGAGAAATAAAATATCTTc                                                                                                                                    >  2:593620/1‑140 (MQ=255)       tGGTAAAATATTTTAAAGATGAGAAACCAACTTATGCTCGAATGACAGCACATCTCCCGAACAAAAATGAGTCTTACGAATGTTTAATAAAGATACAGCATGAACTAATTCGTAGTGAAGAGAAATAAAATATCTTCCTg                                                                                                                                 >  2:396214/1‑140 (MQ=255)       tGGTAAAATATTTTAAAGATGAGAAACCAACTTATGCTCGAATGACAGCACATCTCCCGAACAAAAATGAGTCTTACGAATGTTTAATAAAGATACAGCATGAACTAATTCGTAGTGAAGAGAAATAAAATATCTTCCTg                                                                                                                                 <  1:396214/140‑1 (MQ=255)       tGGTAAAATATTTTAAAGAAGAGAAACCAACTTATGCTCGAATGACAGCACATCTCCCGAACAAAAATGAGTCTTACAAATGTTTAATAAAGATACAGCATGAACTAATTCGTAGTGAAGAGAAATAAAATATCTTCCTg                                                                                                                                 <  2:320086/140‑1 (MQ=255)         gTAAAATATTTTAAAGATGAGAAACCAACTTATGCTCGAATGACAGCACATCTCCCGAACAAAAATGAGTCTTACGAATGTTTAATAAAGATACAGCATGAACTAATTCGTAGTGAAGAGAAATAAAATATCTTCCTgag                                                                                                                               >  1:125250/1‑140 (MQ=255)         gTAAAATATTTTAAAGATGAGAAACCAACTTATGCTCGAATGACAGCACATCTCCCGAACAAAAATGAGTCTTACGAATGTTTAATAAAGATACAGCATGAACTAATTCGTAGTGAAGAGAAATAAAATATCTTCCTgag                                                                                                                               >  1:691105/1‑140 (MQ=255)          tAAAATATTTTAAAGATGAGAAACCAACTTATGCTCGAATGACAGCACATCTCCCGAACAAAAATGAGTCTTACGAATGTTTAATAAAGATACAGCATGAACTAATTCGTAGTGAAGAGAAATAAAATATCTTCCTgaga                                                                                                                              >  1:438116/1‑140 (MQ=255)            aaaTATTTTAAAGATGAGAAACCAACTTATGCTCGAATGACAGCACATCTCCCGAACAAAAATGAGTCTTACGAATGTTTAATAAAGATACAGCATGAACTAATTCGTAGTGAAGAGAAATAAAATATCTTCCTGAGaaa                                                                                                                            <  1:591545/140‑1 (MQ=255)                  tttAAAGATGAGAAACCAACTTATGCTCGAATGACAGCACATCTCCCGAACAAAAATGAGTCTTACGAATGTTTAATAAAGATACAGCATGAACTAATTCGTAGTGAAGAGAAATAAAATATCTTCCTGAGAAAACAAAt                                                                                                                      >  1:69144/1‑140 (MQ=255)                     aaaGATGAGAAACCAACTTATGCTCGAATGACAGCACATCTCCCGAACAAAAATGAGTCTTACGAATGTTTAATAAAGATACAGCATGAACTAATTCGTAGTGAAGAGAAATAAAATATCTTCCTGAGAAAACAAATTaa                                                                                                                   <  2:438116/140‑1 (MQ=255)                      aaGATGAGAAACCAACTTATGCTCGAATGACAGCACATCTCCCGAACAAAAATGAGTCTTACGAATGTTTAATAAAGATACAGCATGAACTAATTCGTAGTGAAGAGAAATAAAATATCTTCCTGAGAAAACAAATTAAt                                                                                                                  <  2:691105/140‑1 (MQ=255)                             gaAACCAACTTATGCTCGAATGACAGCACATCTCCCGAACAAAAATGAGTCTTACGAATGTTTAATAAAGATACAGCATGAACTAATTCGTAGTGAAGAGAAATAAAATATCTTCCTGAGAAAACAAATTAATCATtgtg                                                                                                           <  1:593620/140‑1 (MQ=255)                                aCCAACTTATGCTCGAATGACAGCACATCTCCCGAACAAAAATGAGTCTTACGAATGTTTAATAAAGATACAGCATGAACTAATTCGTAGTGAAGAGAAATAAAATATCTTCCTGAGAAAACAAATTAATCATTGTGaaa                                                                                                        >  2:744934/1‑140 (MQ=255)                                aCCAACTTATGCTCGAATGACAGCACATCTCCCGAACAAAAATGAGTCTTACGAATGTTTAATAAAGATACAGCATGAACTAATTCGTAGTGAAGAGAAATAAAATATCTTCCTGAGAAAACAAATTAATCATTGTGaaa                                                                                                        >  2:789617/1‑140 (MQ=255)                                       tATGCTCGAATGACAGCACATCTCCCGAACAAAAATGAGTCTTACGAATGTTTAATAAAGATACAGCATGAACTAATTCGTAGTGAAGAGAAATAAAATATCTTCCTGAGAAAACAAATTAATCATTGTGAAAAATtata                                                                                                 <  1:475591/140‑1 (MQ=255)                                        aTGCTCGAATGACAGCACATCTCCCGAACAAAAATGAGTCTTACGAATGTTTAATAAAGATACAGCATGAACTAATTCGTAGTGAAGAGAAATAAAATATCTTCCTGAGAAAACAAATTAATCATTGTGAAAAATtatat                                                                                                <  2:539493/140‑1 (MQ=255)                                               aaTGACAGCACATCTCCCGAACAAAAATGAGTCTTACGAATGTTTAATAAAGATACAGCATGAACTAATTCGTAGTGAAGAGAAATAAAATATCTTCCTGAGAAAACAAATTAATCATTGTGAAAAATTATATAACTCa                                                                                          >  2:481566/1‑139 (MQ=255)                                               aaTGACAGCACATCTCCCGAACAAAAATGAGTCTTACGAATGTTTAATAAAGATACAGCATGAACTAATTCGTAGTGAAGAGAAATAAAATATCTTCCTGAGAAAACAAATTAATCATTGTGAAAAATTATATAACTCa                                                                                          <  1:481566/139‑1 (MQ=255)                                                   aCAGCACATCTCCCGAACAAAAATGAGTCTTACGAATGTTTAATAAAGATACAGCATGAACTAATTCGTAGTGAAGAGAAATAAAATATCTTCCTGAGAAAACAAATTAATCATTGTGAAAAATTATATAACTCATGGaa                                                                                     <  1:671096/140‑1 (MQ=255)                                                        acaTCTCCCGAACAAAAATGAGTCTTACGAATGTTTAATAAAGATACAGCATGAACTAATTCGTAGTGAAGAGAAATAAAATATCTTCCTGAGAAAACAAATTAATCATTGTGAAAAATtata                                                                                                 >  2:93110/1‑123 (MQ=255)                                                        acaTCTCCCGAACAAAAATGAGTCTTACGAATGTTTAATAAAGATACAGCATGAACTAATTCGTAGTGAAGAGAAATAAAATATCTTCCTGAGAAAACAAATTAATCATTGTGAAAAATtata                                                                                                 <  1:93110/123‑1 (MQ=255)                                                         caTCTCCCGAACAAAAATGAGTCTTACGAATGTTTAATAAAGATACAGCATGAACTAATTCGTAGTGAAGAGAAATAAAATATCTTCCTGAGAAAACAAATTAATCATTGTGAAAAATTATATAACTCATGGAATAGgtg                                                                               >  1:241899/1‑140 (MQ=255)                                                          aTCTCCCGAACAAAAATGAGTCTTACGAATGTTTAATAAAGATACAGCATGAACTAATTCGTAGTGAAGAGAAATAAAATATCTTCCTGAGAAAACAAATTAATCATTGTGAAAAATTATATAACTCATGGAATAGgtgt                                                                              >  2:532132/1‑140 (MQ=255)                                                              cccGAACAAAAATGAGTCTTACGAATGTTTAATAAAGATACAGCATGAACTAATTCGTAGTGAAGAGAAATAAAATATCTTCCTGAGAAAACAAATTAATCATTGTGAAAAATTATATAACTCATGGAATAGGTGTTTAt                                                                          >  1:485145/1‑140 (MQ=255)                                                                 gAACAAAAATGAGTCTTACGAATGTTTAATAAAGATACAGCATGAACTAATTCGTAGTGAAGAGAAATAAAATATCTTCCTGAGAAAACAAATTAATCATTGTGAAAAATTATATAACTCATGGAATAGGTGTTTAtttt                                                                       >  2:400329/1‑140 (MQ=255)                                                                 gAACAAAAATGAGTCTTACGAATGTTTAATAAAGATACAGCATGAACTAATTCGTAGTGAAGAGAAATAAAATATCTTCCTGAGAAAACAAATTAATCATTGTGAAAAATTATATAACTCATGGAATAGGTGTTTAtttt                                                                       >  1:752500/1‑140 (MQ=255)                                                                  aaCAAAAATGAGTCTTACGAATGTTTAATAAAGATACAGCATGAACTAATTCGTAGTGAAGAGAAATAAAATATCTTCCTGAGAAAACAAATTAATCATTGTGAAAAATTATATAACTCATGGAATAGGTGTTTATTTTg                                                                      >  2:246113/1‑140 (MQ=255)                                                                             gTCTTACGAATGTTTAATAAAGATACAGCATGAACTAATTCGTAGTGAAGAGAAATAAAATATCTTCCTGAGAAAACAAATTAATCATTGTGAAAAATTATATAACTCATGGAATAGGTGTTTATTTTGCGTTCCATTTg                                                           <  1:670016/140‑1 (MQ=255)                                                                             gTCTTACGAATGTTTAATAAAGATACAGCATGAACTAATTCGTAGTGAAGAGAAATAAAATATCTTCCTGAGAAAACAAATTAATCATTGTGAAAAATTATATAACTCATGGAATAGGTGTTTATTTTGCGTTCCATTTg                                                           <  1:165911/140‑1 (MQ=255)                                                                               cTTACGAATGTTTAATAAAGATACAGCATGAACTAATTCGTAGTGAAGAGAAATAAAATATCTTCCTGAGAAAACAAATTAATCATTGTGAAAAATTATATAACTCATGGAATAGGTGTTTATTTTGCGTTCCATTTGCa                                                         <  2:752500/140‑1 (MQ=255)                                                                                 tACGAATGTTTAATAAAGATACAGCATGAACTAATTCGTAGTGAAGAGAAATAAAATATCTTCCTGAGAAAACAAATTAATCATTGTG‑‑AAATTATATAACTCATGGAATAGGTGTTTATTTTGCGTTCCATTTGCAGGGa                                                     >  2:651984/1‑140 (MQ=255)                                                                                   cGAATGTTTAATAAAGATACAGCATGAACTAATTCGTAGTGAAGAGAAATAAAATATCTTCCTGAGAAAACAAATTAATCATTGTGAAAAATTATATAACTCATGGAATAGGTGTTTATTTTGCGTTCCATTTGCAGGGa                                                     <  2:125250/140‑1 (MQ=255)                                                                                   cGAATGTTTAATAAAGATACAGCATGAACTAATTCGTAGTGAAGAGAAATAAAATATCTTCCTGAGAAAACAAATTAATCATTGTGAAAAATTATATAACTCATGGAATAGGTGTTTATTTTGCGTTCCATTTGCAGGGa                                                     <  1:647540/140‑1 (MQ=255)                                                                                   cGAATGTTTAATAAAGATACAGCATGAACTAATTCGTAGTGAAGAGAAATAAAATATCTTCCTGAGAAAACAAATTAATCATTGTGAAAAATTATATAACTCATGGAATAGGTGTTTATTTTGCGTTCCATTTGCAGGGa                                                     <  2:430260/140‑1 (MQ=255)                                                                                        gTTTAATAAAGATACAGCATGAACTAATTCGTAGTGAAGAGAAATAAAATATCTTCCTGAGAAAACAAATTAATCATTGTGAAAAATTATATAACTCATGGAATAGATGTTTATTTTGCGTTCCATTTGCAGGGAAAGAt                                                >  1:634797/1‑140 (MQ=255)                                                                                           taataaAGATACAGCATGAACTAATTCGTAGTGAAGAGAAATAAAATATCTTCCTGAGAAAACAAATTAATCATTGTGAAAAATTATATAACTCATGGAATAGGTGTTTATTTTGCGTTCCATTTGCAGGGAAAGATCAc                                             >  2:740732/1‑140 (MQ=255)                                                                                           taataaAGATACAGCATGAACTAATTCGTAGTGAAGAGAAATAAAATATCTTCCTGAGAAAACAAATTAATCATTGTGAAAAATTATATAACTCATGGAATAGGTGTTTATTTTGCGTTCCATTTGCAGGGAAAGATCAc                                             >  1:799453/1‑140 (MQ=255)                                                                                            aataaAGATACAGCATGAACTAATTCGTAGTGAAGAGAAATAAAATATCTTCCTGAGAAAACAAATTAATCATTGTGAAAAATTATATAACTCATGGAATAGGTGTTTATTTTGCGTTCCATTTGCAGGGAAAGATCACg                                            <  2:485145/140‑1 (MQ=255)                                                                                             ataaAGATACAGCATGAACTAATTCGTAGTGAAGAGAAATAAAATATCTTCCTGAGAAAACAAATTAATCATTGTGAAAAATTATATAACTCATGGAATAGGTGTTTATTTTGCGTTCCATTTGCAGGGAAAGATCACGt                                           >  2:401702/1‑140 (MQ=255)                                                                                               aaaGATACAGCATGAACTAATTCGTAGTGAAGAGAAATAAAATATCTTCCTGAGAAAACAAATTAATCATTGTGAAAAATTATATAACTCATGGAATAGGTGTTTATTTTGCGTTCCATTTGCAGGGAAAGATCACGTaa                                         <  1:789617/140‑1 (MQ=255)                                                                                               aaaGATACAGCATGAACTAATTCGTAGTGAAGAGAAATAAAATATCTTCCTGAGAAAACAAATTAATCATTGTGAAAAATTATATAACTCATGGAATAGGTGTTTATTTTGCGTTCCATTTGCAGGGAAAGATCACGTaa                                         <  1:744934/140‑1 (MQ=255)                                                                                                 aGATACAGCATGAACTAATTCGTAGTGAAGAGAAATAAAATATCTTCCTGAGAAAACAAATTAATCATTGTGAAAAATTATATAACTCATGGAATAGGTGTTTATTTTGCGTTCCATTTGCAGGGAAAGATCACGTAACg                                       <  1:401702/140‑1 (MQ=255)                                                                                                   aTACAGCATGAACTAATTCGTAGTGAAGAGAAATAAAATATCTTCCTGAGAAAACAAATTAATCATTGTGAAAAATTATATAACTCATGGAATAGGTGTTTATTTTGCGTTCCATTTGCAGGGAAAGATCACg                                            >  2:713085/1‑133 (MQ=255)                                                                                                   aTACAGCATGAACTAATTCGTAGTGAAGAGAAATAAAATATCTTCCTGAGAAAACAAATTAATCATTGTGAAAAATTATATAACTCATGGAATAGGTGTTTATTTTGCGTTCCATTTGCAGGGAAAGATCACg                                            <  1:713085/133‑1 (MQ=255)                                                                                                   aTACAGCATGAACTAATTCGTAGTGAAGAGAAATAAAATATCTTCCTGAGAAAACAAATTAATCATTGTGAAAAATTATATAACTCATGGAATAGGTGTTTATTTTGCGTTCCATTTGCAGGGAAAGATCACGTAACGCt                                     <  2:550141/140‑1 (MQ=255)                                                                                                   aTACAACATGAACTAATTCGTAGTGAAGAGAAATAAAATATCTTCCTGAGAAAACAAATTAATCATTGTGAAAAATTATATAACTCATGGAATAGGGGTTTATTTTTCGTTCCATTTTCAGGGAAAAATCACGTAACGCt                                     >  2:709212/1‑140 (MQ=255)                                                                                                    tACAGCATGAACTAATTCGTAGTGAAGAGAAATAAAATATCTTCCTGAGAAAACAAATTAATCATTGTGAAAAATTATATAACTCATGGAATAGGTGTTTATTTTGCGTTCCATTTGCAGGGAAAGATCACGTAACGCTa                                    <  1:110805/140‑1 (MQ=255)                                                                                                    tACAGCATGAACTAATTCGTAGTGAAGAGAAATAAAATATCTTCCTGAGAAAACAAATTAATCATTGTGAAAAATTATATAACTCATGGAATAGGTGTTTATTTTGCGTTCCATTTGCAGGGAAAGATCACGTAACGCTa                                    <  2:454822/140‑1 (MQ=255)                                                                                                       aGCATGAACTAATTCGTAGTGAAGAGAAATAAAATATCTTCCTGAGAAAACAAATTAATCATTGTGAAAAATTATATAACTCATGGAATAGGTGTTTATTTTGCGTTCCATTTGCAGGGAAAGATCACGTAACGCTACtt                                 >  2:292654/1‑140 (MQ=255)                                                                                                            gAACTAATTCGTAGTGAAGAGAAATAAAATATCTTCCTGAGAAAACAAATTAATCATTGTGAAAAATTATATAACTCATGGAATAGGTGTTTATTTTGCGTTCCATTTGCAGGGAAAGATCACGTAACGCTACTTTTTTg                            >  2:534602/1‑140 (MQ=255)                                                                                                            gAACTAATTCGTAGTGAAGAGAAATAAAATATCTTCCTGAGAAAACAAATTAATCATTGTGAAAAATTATATAACTCATGGAATAGGTGTTTATTTTGCGTTCCATTTGCAGGGAAAGATCACGTAACGCTACTTTTTTg                            >  2:122431/1‑140 (MQ=255)                                                                                                             aaCTAATTCGTAGTGAAGAGAAATAAAATATCTTCCTGAGAAAACAAATTAATCATTGTGAAGAATTATATAACTCATGGAATAGGTGTTTATTTTGCGTTCCATTTGCAGGGAAAGATCACGTAACGCTACTTTTTTGt                           >  1:288507/1‑140 (MQ=255)                                                                                                             aaCTAATTCGTAGTGAAGAGAAATAAAATATCTTCCTGAGAAAACAAATTAATCATTGTGAAAAATTATATAACTCATGGAATAGGTGTTTATTTTGCGTTCCATTTGCAGGGAAAGATCACGTAACGCTACTTTTTTGt                           >  2:661632/1‑140 (MQ=255)                                                                                                              aCTAATTCGTAGTGAAGAGAAATAAAATATCTTCCTGAGAAAACAAATTAATCATTGTGAAAAATTATATAACTCATGGAATAGGTGTTTATTTTGCGTTCCATTTGCAGGGAAAGATCACGTAACGCTACTTTTTTGTa                          >  1:359780/1‑140 (MQ=255)                                                                                                                       tAGTGAAGAGAAATAAAATATCTTCCTGAGAAAACAAATTAATCATTGTGAAAAATTATATAACTCATGGAATAGGTGTTTATTTTGCGTTCCATTTGCAGGGAAAGATCACGTAACGCTACTTTTTTGTACTAaataat                 >  2:699347/1‑140 (MQ=255)                                                                                                                       tAGTGAAGAGAAATAAAATATCTTCCTGAGAAAACAAATTAATCATTGTGAAAAATTATATAACTCATGGAATAGGTGTTTATTTTGCGTTCCATTTGCAGGGAAAGATCACGTAACGCTACTTTTTTGTACTAaataat                 >  2:320737/1‑140 (MQ=255)                                                                                                                       tAGTGAAGAGAAATAAAATATCTTCCTGAGAAAACAAATTAATCATTGTGAAAAATTATATAACTCATGGAATAGGTGTTTATTTTGCGTTCCATTTGCAGGGAAAGATCACGTAACGCTACTTTTTTGTACTAaataat                 >  1:129841/1‑140 (MQ=255)                                                                                                                          tGAAGAGAAATAAAATATCTTCCTGAGAAAACAAATTAATCATTGTGAAAAATTATATAACTCATGGAATAGGTGTTTATTTTGCGTTCCATTTGCAGGGAAAGATCACGTAACGCTACTTTTTTGTACTAAATAATTCg              <  1:661632/140‑1 (MQ=255)                                                                                                                             agagAAATAAAATATCTTCCTGAGAAAACAAATTAATCATTGTGAAAAATTATATAACTCATGGAATAGGTGTTTATTTTGCGTTCCATTTGCAGGGAAAGATCACGTAACGCTACTTTTTTGTACTAAATAATTCGAAt           >  1:386715/1‑140 (MQ=255)                                                                                                                              gagaAATAAAATATCTTCCTGAGAAAACAAATTAATCATTGTGAAAAATTATATAACTCATGGAATAGGTGTTTATTTTGCGTTCCATTTGCAGGGAAAGATCACGTAACGCTACTTTTTTGTACTAAATAATTCGAAtt          >  1:37896/1‑140 (MQ=255)                                                                                                                              gagaAATAAAATATCTTCCTGAGAAAACAAATTAATCATTGTGAAAAATTATATAACTCATGGAATAGGTGTTTATTTTGCGTTCCATTTGCAGGGAAAGATCACGTAACGCTACTTTTTTGTACTAAATAATTCGAAtt          >  2:225322/1‑140 (MQ=255)                                                                                                                                      aaaTATCTTCCTGAGAAAACAAATTAATCATTGTGAAAAATTATATAACTCATGGAATAGGTGTTTATTTTGCGTTCCATTTGCAGGGAAAGATCACGTAACGCTACTTTTTTGTACTAAATAATTCGAATTTTAAGTTc  >  2:167095/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                        CAATCATGGTAAAATATTTTAAAGATGAGAAACCAACTTATGCTCGAATGACAGCACATCTCCCGAACAAAAATGAGTCTTACGAATGTTTAATAAAGATACAGCATGAACTAATTCGTAGTGAAGAGAAATAAAATCTCTTCCTGAGAAAACAAATTAATCATTGTGAAAAATTATATAACTCATGGAATAGGTGTTTATTTTGCGTTCCATTTGCAGGGAAAGATCACGTAACGCTACTTTTTTGTACTAAATAATTCGAATTTTAAGTTC  >  NC_000913/736086‑736358

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑