Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,182,197	G→T	S211S (TCC→TCA)	potD ←	spermidine preferential ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,182,197	0	G	T	100.0%	177.2 / NA	50	S211S (TCC→TCA)	potD	spermidine preferential ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (24/26);  total (24/26)

TCCATCCAGAAAATGCCGCCTTCTTTCGGCCACACCACGTCAATTGGCGTACCCGCCTGGCGTGCAACGAAAGCAGAACCGTTCCAGATCATGCCGAGGTTAACTTCGCCTTCCATGTACGGGTTCGCCGGGTTATCGGAGTTAAAGGCTGCCACGTTTGGCATCAGTTTTTTCAGCTCGTTATATGCAGCTTCAATCTCTTTCGGATCGGTGGTGTTACCGGAGTAGCCCAGCTTACGCAGCGCCATCTGGAACACTTCACGGGCATCGT  >  NC_000913/1182060‑1182330                                                                                                                                          |                                                                                                                                      tccatccaGAAAATGCCGCCTTCTTTCGGCCACACCACGTCAATTGGCGTACCCGCCTGGCGTGCAACGAAAGCAGAACCGTTCCAGATCATGCCGAGGTTAACTTCGCCTTCCATGTACGGGTTCGCCGGGTTATCTGa                                                                                                                                     >  1:75551/1‑140 (MQ=255) tccatccaGAAAATGCCGCCTTCTTTCGGCCACACCACGTCAATTGGCGTACCCGCCTGGCGTGCAACGAAAGCAGAACCGTTCCAGATCATGCCGAGGTTAACTTCGCCTTCCATGTACGGGTTCGCCGGGTTATCTGa                                                                                                                                     >  1:279150/1‑140 (MQ=255)      ccaGAAAATGCCGCCTTCTTTCGGCCACACCACGTCAATTGGCGTACCCGCCTGGCGTGCAACGAAAGCAGAACCGTTCCAGATCATGCCGAGGTTAACTTCGCCTTCCATGTACGGGTTCGCCGGGTTATCTGAGTTaa                                                                                                                                >  2:81609/1‑140 (MQ=255)           aaaTGCCGCCTTCTTTCGGCCACACCACGTCAATTGGCGTACCCGCCTGGCGTGCAACGAAAGCAGAACCGTTCCAGATCATGCCGAGGTTAACTTCGCCTTCCATGTACGGGTTCGCCGGGTTATCTGAGTTAAAGGCt                                                                                                                           >  2:11795/1‑140 (MQ=255)                        tttCGGCCACACCACGTCAATTGGCGTACCCGCCTGGCGTGCAACGAAAGCAGAACCGTTCCAGATCATGCCGAGGTTAACTTCGCCTTCCATGTACGGGTTCGCCGGGTTATCTGAGTTAAAGGCTGCCACGTTTGGCa                                                                                                              <  2:641363/140‑1 (MQ=255)                        tttCGGCCACACCACGTCAATTGGCGTACCCGCCTGGCGTGCAACGAAAGCAGAACCGTTCCAGATCATGCCGAGGTTAACTTCGCCTTCCATGTACGGGTTCGCCGGGTTATCTGAGTTAAAGGCTACCACGTTTGGCa                                                                                                              <  1:67577/140‑1 (MQ=255)                             gCCACACCACGTCAATTGGCGTACCCGCCTGGCGTGCAACGAAAGCAGAACCGTTCCAGATCATGCCGAGGTTAACTTCGCCTTCCATGTACGGGTTCGCCGGGTTATCTGAGTTAAAGGCTGCCACGTTTGGCATCAGt                                                                                                         >  1:432822/1‑140 (MQ=255)                                  accacGTCAATTGGCGTACCCGCCTGGCGTGCAACGAAAGCAGAACCGTTCCAGATCATGCCGAGGTTAACTTCGCCTTCCATGTACGGGTTCGCCGGGTTATCTGAGTTAAAGGCTGCCACGTTTGGCATCAGtttttt                                                                                                    >  2:520469/1‑140 (MQ=255)                                      catCAATTGGCGTACCCGCCTGGCGTGCAACGAAAGCAGAACCGTTCCAGATCATGCCGAGGTTAACTTCGCCTTCCATGTACGGGTTCGCCGGGTTATCTGAGTTAAAGGCTGCCACGTTTGGCATCAGTTTTTTCAGc                                                                                                <  1:504375/138‑1 (MQ=255)                                      cGTCAATTGGCGTACCCGCCTGGCGTGCAACGAAAGCAGAACCGTTCCAGATCATGCCGAGGTTAACTTCGCCTTCCATGTACGGGTTCGCCGGGTTATCTGAGTTAAAGGCTGCCACGTTTGGCATCAGTTTTTTCAGc                                                                                                <  1:11795/140‑1 (MQ=255)                                      cGTCAATTGGCGTACCCGCCTGGCGTGCAACGAAAGCAGAACCGTTCCAGATCATGCCGAGGTTAACTTCGCCTTCCATGTACGGGTTCGCCGGGTTATCTGAGTTAAAGGCTGCCACGTTTGGCATCAGTTTTTTCAGc                                                                                                <  2:312699/140‑1 (MQ=255)                                      cGTCAATTGGCGTACCCGCCTGGCGTGCAACGAAAGCAGAACCGTTCCAGATCATGCCGAGGTTAACTTCGCCTTCCATGTACGGGTTCGCCGGGTTATCTGAGTTAAAGGCTGCCACGTTTGGCATCAGTTTTTTCAGc                                                                                                >  1:177776/1‑140 (MQ=255)                                      cGTCAATTGGCGTACCCGCCTGGCGTGCAACGAAAGCAGAACCGTTCCAGATCATGCCGAGGTTAACTTCGCCTTCCATGTACGGGTTCGCCGGGTTATCTGAGTTAAAGGCTGCCACGTTTGGCATCAGTTTTTTCAGc                                                                                                <  2:75551/140‑1 (MQ=255)                                      cGTCAATTGGCGTACCCGCCTGGCGTGCAACGAAAGCAGAACCGTTCCAGATCATGCCGAGGTTAACTTCGCCTTCCATGTACGGGTTCGCCGGGTTATCTGAGTTAAAGGCTGCCACGTTTGGCATCAGTTTTTTCAGc                                                                                                <  1:520469/140‑1 (MQ=255)                                       gTCAATTGGCGTACCCGCCTGGCGTGCAACGAAAGCAGAACCGTTCCAGATCATGCCGAGGTTAACTTCGCCTTCCATGTACGGGTTCGCCGGGTTATCTGAGTTAAAGGCTGCCACGTTTGGCATCAGTTTTTTCAGCt                                                                                               >  1:594330/1‑140 (MQ=255)                                           aTTGGCGTACCCGCCTGGCGTGCAACGAAAGCAGAACCGTTCCAGATCATGCCGAGGTTAACTTCGCCTTCCATGTACGGGTTCGCCGGGTTATCTGAGTTAAAGGCTGCCACGTTTGGCATCAGTTTTTTCAGCTCGtt                                                                                           <  2:177776/140‑1 (MQ=255)                                           aTTGGCGTACCCGCCTGGCGTGCAACGAAAGCAGAACCGTTCCAGATCATGCCGAGGTTAACTTCGCCTTCCATGTACGGGTTCGCCGGGTTATCTGAGTTAAAGGCTGCCACGTTTGGCATCAGTTTTTTCAGCTCGtt                                                                                           <  1:210218/140‑1 (MQ=255)                                              ggCGTACCCGCCTGGCGTGCAACGAAAGCAGAACCGTTCCAGATCATGCCGAGGTTAACTTCGCCTTCCATGTACGGGTTCGCCGGGTTATCTGAGTTAAAGGCTGCCACGTTTGGCATCAGTTTTTTCAGCTCGTtata                                                                                        <  1:628355/140‑1 (MQ=255)                                                cGTACCCGCCTGGCGTGCAACGAAAGCAGAACCGTTCCAGATCATGCCGAGGTTAACTTCGCCTTCCATGTACGGGTTCGCCGGGTTATCTGAGTTAAAGGCTGCCACGTTTGGCATCAGTTTTTTCAGCTCGTTATATg                                                                                      <  1:424672/140‑1 (MQ=255)                                                   aCCCGCCTGGCGTGCAACGAAAGCAGAACCGTTCCAGATCATGCCGAGGTTAACTTCGCCTTCCATGTACGGGTTCGCCGGGTTATCTGAGTTAAAGGCTGCCACGTTTGGCATCAGTTTTTTCAGCTCGTTATATGCAg                                                                                   >  2:4279/1‑140 (MQ=255)                                                        ccTGGCGTGCAACGAAAGCAGAACCGTTCCAGATCATGCCGAGGTTAACTTCGCCTTCCATGTACGGGTTCGCCGGGTTATCTGAGTTAAAGGCTGCCACGTTTGGCATCAGTTTTTTCAGCTCGTTATATGCAGCTTCa                                                                              >  2:337737/1‑140 (MQ=255)                                                          tGGCGTGCAACGAAAGCAGAACCGTTCCAGATCATGCCGAGGTTAACTTCGCCTTCCATGTACGGGTTCGCCGGGTTATCTGAGTTAAAGGCTGCCACGTTTGGCATCAGTTTTTTCAGCTCGTTATATGCAGCTTCAAt                                                                            <  2:432822/140‑1 (MQ=255)                                                          tGGCGTGCAACGAAAGCAGAACCGTTCCAGATCATGCCGAGGTTAACTTCGCCTTCCATGTACGGGTTCGCCGGGTTATCTGAGTTAAAGGCTGCCACGTTTGGCATCAGTTTTTTCAGCTCGTTATATGCAGCTTCAAt                                                                            >  2:192418/1‑140 (MQ=255)                                                          tGGCGTGCAACGAAAGCAGAACCGTTCCAGATCATGCCGAGGTTAACTTCGCCTTCCATGTACGGGTTCGCCGGGTTATCTGAGTTAAAGGCTGCCACGTTTGGCATCAGTTTTTTCAGCTCGTTATATGCAGCTTCAAt                                                                            <  2:492681/140‑1 (MQ=255)                                                              gTGCAACGAAAGCAGAACCGTTCCAGATCATGCCGAGGTTAACTTCGCCTTCCATGTACGGGTTCGCCGGGTTATCTGAGTTAAAGGCTGCCACGTTTGGCATCAGTTTTTTCAGCTCGTTATATGCAGCtt                                                                                <  1:771261/132‑1 (MQ=255)                                                              gTGCAACGAAAGCAGAACCGTTCCAGATCATGCCGAGGTTAACTTCGCCTTCCATGTACGGGTTCGCCGGGTTATCTGAGTTAAAGGCTGCCACGTTTGGCATCAGTTTTTTCAGCTCGTTATATGCAGCtt                                                                                >  2:771261/1‑132 (MQ=255)                                                               tGCAACGAAAGCAGAACCGTTCCAGATCATGCCGAGGTTAACTTCGCCTTCCATGTACGGGTTCGCCGGGTTATCTGAGTTAAAGGCTGCCACGTTTGGCATCAGTTTTTTCAGCTCGTTATATGCAGCTTCAATCTCtt                                                                       >  2:696365/1‑140 (MQ=255)                                                                 cAACGAAAGCAGAACCGTTCCAGATCATGCCGAGGTTAACTTCGCCTTCCATGTACGGGTTCGCCGGGTTATCTGAGTTAAAGGCTGCCACGTTTGGCATCAGTTTTTTCAGCTCGTTATATGCAGCTTCAATCTCTTTc                                                                     >  2:79818/1‑140 (MQ=255)                                                                  aaCGAAAGCAGAACCGTTCCAGATCATGCCGAGGTTAACTTCGCCTTCCATGTACGGGTTCGCCGGGTTATCTGAGTTAAAGGCTGCCACGTTTGGCATCAGTTTTTTCAGCTCGTTATATGCAGCTTCAATCTCTTTCg                                                                    >  1:623718/1‑140 (MQ=255)                                                                   aCGAAAGCAGAACCGTTCCAGATCATGCCGAGGTTAACTTCGCCTTCCATGTACGGGTTCGCCGGGTTATCTGAGTTAAAGGCTGCCACGTTTGGCATCAGTTTTTTCAGCTCGTTATATGCAGCTTCAATCTCTTTCgg                                                                   <  1:4279/140‑1 (MQ=255)                                                                     gAAAGCAGAACCGTTCCAGATCATGCCGAGGTTAACTTCGCCTTCCATGTACGGGTTCGCCGGGTTATCTGAGTTAAAGGCTGCCACGTTTGGCATCAGTTTTTTCAGCTCGTTATATGCAGCTTCAATCTCTTTCGGAt                                                                 <  1:337737/140‑1 (MQ=255)                                                                            gAACCGTTCCAGATCATGCCGAGGTTAACTTCGCCTTCCATGTACGGGTTCGCCGGGTTATCTGAGTTAAAGGCTGCCACGTTTGGCATCAGTTTTTTCAGCTCGTTATATGCAGCTTCAATCTCTTTCGGATCggtggt                                                          >  1:257343/1‑140 (MQ=255)                                                                            gAACCGTTCCAGATCATGCCGAGGTTAACTTCGCCTTCCATGTACGGGTTCGCCGGGTTATCTGAGTTAAAGGCTGCCACGTTTGGCATCAGTTTTTTCAGCTCGTTATATGCAGCTTCAATCTCTTTCGGATCggtggt                                                          <  2:411203/140‑1 (MQ=255)                                                                            gAACCGTTCCAGATCATGCCGAGGTTAACTTCGCCTTCCATGTACGGGTTCGCCGGGTTATCTGAGTTAAAGGCTGCCACGTTTGGCATCAGTTTTTTCAGCTCGTTATATGCAGCTTCAATCTCTTTCGGATCggtggt                                                          <  1:696365/140‑1 (MQ=255)                                                                                  ttCCAGATCATGCCGAGGTTAACTTCGCCTTCCATGTACGGGTTCGCCGGGTTATCTGAGTTAAAGGCTGCCACGTTTGGCATCAGTTTTTTCAGCTCGTTATATGCAGCTTCAATCTCTTTCGGATCGGTGGTGTTAcc                                                    >  1:321633/1‑140 (MQ=255)                                                                                     cAGATCATGCCGAGGTTAACTTCGCCTTCCATGTACGGGTTCGCCGGGTTATCTGAGTTAAAGGCTGCCACGTTTGGCATCAGTTTTTTCAGCTCGTTATATGCAGCTTCAATCTCTTTCGGATCGGTGGTGTTACCGGa                                                 <  2:594330/140‑1 (MQ=255)                                                                                          cATGCCGAGGTTAACTTCGCCTTCCATGTACGGGTTCGCCGGGTTATCTGAGTTAAAGGCTGCCACGTTTGGCATCAGTTTTTTCAGCTCGTTATTTGCAGCTTCAATCTCTTTCGGATCGGTGGTGTTACCGGAGTAGc                                            <  1:475040/140‑1 (MQ=255)                                                                                             gCCGAGGTTAACTTCGCCTTCCATGTACGGGTTCGCCGGGTTATCTGAGTTAAAGGCTGCCACGTTTGGCATCAGTTTTTTCAGCTCGTTATATGCAGCTTCAATCTCTTTCGGATCGGTGGTGTTACCGTAGTAGCCCa                                         >  1:765798/1‑140 (MQ=255)                                                                                                  ggTTAACTTCGCCTTCCATGTACGGGTTCGCCGGGTTATCTGAGTTAAAGGCTGCCACGTTTGGCATCAGTTTTTTCAGCTCGTTATATGCAGCTTCAATCTCTTTCGGATCGGTGGTGTTACCGGAGTAGcc                                           <  2:691584/133‑1 (MQ=255)                                                                                                  ggTTAACTTCGCCTTCCATGTACGGGTTCGCCGGGTTATCTGAGTTAAAGGCTGCCACGTTTGGCATCAGTTTTTTCAGCTCGTTATATGCAGCTTCAATCTCTTTCGGATCGGTGGTGTTACCGGAGTAGcc                                           >  1:691584/1‑133 (MQ=255)                                                                                                  ggTTAACTTCGCCTTCCATGTACGGGTTCGCCGGGTTATCTGAGTTAAAGGCTGCCACGTTTGGCATCAGTTTTTTCAGCTCGTTATATGCAGCTTCAATCTCTTTCGGATCGGTGGTGTTACCGGAGTAGCCCAGCTTa                                    >  1:635848/1‑140 (MQ=255)                                                                                                      aaCTTCGCCTTCCATGTACGGGTTCGCCGGGTTATCTGAGTTAAAGGCTGCCACGTTTGGCATCAGTTTTTTCAGCTCGTTATATGCAGCTTCAATCTCTTTCGGATCGGTGGTGTTACCGGAGTAGCCCAGCTTACGCa                                <  1:81609/140‑1 (MQ=255)                                                                                                         ttCGCCTTCCATGTACGGGTTCGCCGGGTTATCTGAGTTAAAGGCTGCCACGTTTGGCATCAGTTTTTTCAGCTCGTTATATGCAGCTTCAATCTCTTTCGGATCGGTGGTGTTACCGGAGTAGCCCAGCTTACGCAgcg                             <  2:257343/140‑1 (MQ=255)                                                                                                                   aTGTACGGGTTCGCCGGGTTATCTGAGTTAAAGGCTGCCACGTTTGGCATCAGTTTTTTCAGCTCGTTATATGCAGCTTCAATCTCTTTCGGATCGGTGGTGTTACCGTAGTAGCCCAGCTTACGCAGCGCCATCTGGaa                   <  2:765798/140‑1 (MQ=255)                                                                                                                   aTGTACGGGTTCGCCGGGTTATCTGAGTTAAAGGCTGCCACGTTTGGCATCAGTTTTTTCAGCTCGTTATATGCAGCTTCAATCTCTTTCGGATCGGTGGTGTTACCGGAGTAGCCCAGCTTACGCAGCGCCATCTGGaa                   <  1:192418/140‑1 (MQ=255)                                                                                                                           gTTCGCCGGGTTATCTGAGTTAAAGGCTGCCACGTTTGGCATCAGTTTTTTCAGCTCGTTATATGCAGCTTCAATCTCTTTCGGATCGGTGGTGTTACCGGAGTAGCCCAGCTTACGCAGCGCCATCTGGAACACTTCAc           >  1:212672/1‑140 (MQ=255)                                                                                                                              cGCCGGGTTATCTGAGTTAAAGGCTGCCACGTTTGGCATCAGTTTTTTCAGCTCGTTATATGCAGCTTCAATCTCTTTCGGATCGGTGGTGTTACCGGAGTAGCCCAGCTTACGCAGCGCCATCTGGAACACTTCACggg        >  1:728299/1‑140 (MQ=255)                                                                                                                              cGCCGGGTTATCTGAGTTAAAGGCTGCCACGTTTGGCATCAGTTTTTTCAGCTCGTTATATGCAGCTTCAATCTCTTTCGGATCGGTGGTGTTACCGGAGTAGCCCAGCTTACGCAGCGCCATCTGGAACACTTCACggg        >  1:713391/1‑140 (MQ=255)                                                                                                                              cGCCGGGTTATCTGAGTTAAAGGCTGCCACGTTTGGCATCAGTTTTTTCAGCTCGTTATATGCAGCTTCAATCTCTTTCGGATCGGTGGTGTTACCGGAGTAGCCCAGCTTACGCAGCGCCATCTGGAACACTTCACggg        >  1:401706/1‑140 (MQ=255)                                                                                                                                    gTTATCTGAGTTAAAGGCTGCCACGTTTGGCATCAGTTTTTTCAGCTCGTTATATGCAGCTTCAATCTCTTTCGGATCGGTGGTGTTACCGGAGTAGCCCAGCTTACGCAGCGCCATCTGGAACACTTCACGGGCAtcgt  <  2:321633/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                      TCCATCCAGAAAATGCCGCCTTCTTTCGGCCACACCACGTCAATTGGCGTACCCGCCTGGCGTGCAACGAAAGCAGAACCGTTCCAGATCATGCCGAGGTTAACTTCGCCTTCCATGTACGGGTTCGCCGGGTTATCGGAGTTAAAGGCTGCCACGTTTGGCATCAGTTTTTTCAGCTCGTTATATGCAGCTTCAATCTCTTTCGGATCGGTGGTGTTACCGGAGTAGCCCAGCTTACGCAGCGCCATCTGGAACACTTCACGGGCATCGT  >  NC_000913/1182060‑1182330

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑