Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,151,853	G→T	P207Q (CCG→CAG)	pphC ←	protein‑serine/threonine phosphatase PphC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,151,853	0	G	T	100.0%	112.8 / NA	33	P207Q (CCG→CAG)	pphC	protein‑serine/threonine phosphatase PphC
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (12/21);  total (12/21)

CCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGATACCATCCGTAAATGCTGCAACTTTGTGCGCACGCCCAGTGCTGGTGAAAGTTTCCAGTCTGGAAACGGCATCTTC  >  NC_000913/2151720‑2151986                                                                                                                                      |                                                                                                                                      ccACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACTGGGTaa                                                                                                                                 <  1:503910/140‑1 (MQ=255)   acaGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACTGGGTaaag                                                                                                                               <  1:548185/140‑1 (MQ=255)      gcgcCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACTGGGTaaagaaa                                                                                                                            <  1:111390/140‑1 (MQ=255)        gcCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACTGGGTAAAGAAAgg                                                                                                                          >  2:153926/1‑140 (MQ=255)        gcCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACTGGGTAAAGAAAgg                                                                                                                          <  2:150904/140‑1 (MQ=255)          cAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACTGGGTAAAGAAAGGca                                                                                                                        >  2:459637/1‑140 (MQ=255)          cAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACTGGGTAAAGAAAGGca                                                                                                                        >  1:568339/1‑140 (MQ=255)          cAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACTGGGTAAAGAAAGGca                                                                                                                        >  2:613917/1‑140 (MQ=255)           aGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTTCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACTGGGTAAAGAAAGGcac                                                                                                                       <  1:349854/140‑1 (MQ=255)           aGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACTGGGTAAAGAAAGGcac                                                                                                                       <  1:756396/140‑1 (MQ=255)                cAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACTGGGTAAAGAAAGGCACATgag                                                                                                                  <  2:156195/140‑1 (MQ=255)                cAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACTGGGTAAAGAAAGGCACATgag                                                                                                                  <  1:726886/140‑1 (MQ=255)                          tcatcatcCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACTGGGTAAAGAAAGGCACATGAGGAGAATTATc                                                                                                        <  2:568339/140‑1 (MQ=255)                          tcatcatcCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACTGGGTAAAGAAAGGCACATGAGGAGAATTATc                                                                                                        <  1:73597/140‑1 (MQ=255)                              catcCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACTGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGc                                                                                                    <  1:613917/140‑1 (MQ=255)                              catcCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACTGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGc                                                                                                    <  1:459637/140‑1 (MQ=255)                                tcCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACTGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCat                                                                                                  >  2:560654/1‑140 (MQ=255)                                tcCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACTGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCa                                                                                                   <  2:287274/139‑1 (MQ=255)                                tcCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACTGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCa                                                                                                   >  1:287274/1‑139 (MQ=255)                                        cTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACTGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACg                                                                                          >  1:440790/1‑140 (MQ=255)                                         tCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACTGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGc                                                                                                    <  2:808312/129‑1 (MQ=255)                                         tCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACTGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGc                                                                                                    >  1:808312/1‑129 (MQ=255)                                          cGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACTGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGcc                                                                                        <  1:153926/140‑1 (MQ=255)                                             ttACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACTGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGt                                                                                     <  1:560654/140‑1 (MQ=255)                                                      ggCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACTGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGATa                                                                            <  2:100869/140‑1 (MQ=255)                                                      ggCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACTGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGATa                                                                            <  1:860008/140‑1 (MQ=255)                                                                                     taataaATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACTGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGATACCATCCGTAAATGCTGCAACTTTGTGCGCAc                                             >  2:705137/1‑140 (MQ=255)                                                                                      aataaATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACTGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGATACCATCCGTAAATGCTGCAACTTTGTGCGCACg                                            >  2:623360/1‑140 (MQ=255)                                                                                              gAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACTGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGATACCATCCGTAAATGCTGCAACTTTGTGCGCACGCCCAGTGc                                    >  2:161904/1‑140 (MQ=255)                                                                                                              gctgcCGCCAGTCCATTGAAAAACTGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGATACCATCCGTAAATGCTGCAACTTTGTGCGCACGCCCAGTGCTGGTGAAAGTTTCCAg                    <  1:623360/140‑1 (MQ=255)                                                                                                              gctgcCGCCAGTCCATTGAAAAACTGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGATACCATCCGTAAATGCTGCAACTTTGTGCGCACGCCCAGTGCTGGTGAAAGTTTCCAg                    >  1:605085/1‑140 (MQ=255)                                                                                                                    gccAGTCCATTGAAAAACTGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGATACCATCCGTAAATGCTGCAACTTTGTGCGCACGCCCAGTGCTGGTGAAAGTTTCCAGTCTGGa              <  1:807412/140‑1 (MQ=255)                                                                                                                                aaaaaCTGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGATACCATCCGTAAATGCTGCAACTTTGTGCGCACGCCCAGTGCTGGTGAAAGTTTCCAGTCTGGAAACGGCATCTtc  <  2:440790/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                                      CCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGATACCATCCGTAAATGCTGCAACTTTGTGCGCACGCCCAGTGCTGGTGAAAGTTTCCAGTCTGGAAACGGCATCTTC  >  NC_000913/2151720‑2151986

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑