Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	389,699	C→A	R10L (CGC→CTC)	hemB ←	porphobilinogen synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	389,699	0	C	A	100.0%	148.0 / NA	43	R10L (CGC→CTC)	hemB	porphobilinogen synthase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (21/22);  total (21/22)

GCATCACGCCTGGCATGGCTTCAACGGCTTTGTAGTCGTCAATTTCTTCTTCAACAAAGATCGGCAACACCAGGTCGTTAAGGCTAAGTGTTGTCTCTTCAAACATAGCGCGCAGCGCAGGAGATTTGCGCAGGCGACGAGGGCGTTGGATTAAGTCTGTCATGGTCTGCCTGATGTTTGTGGAATCGAGGGGCATAGTATACCTGAAGCAGGGTAGGGATGTTTTACGAAAGTTGTCGCGATGTTGACAAGAAGAGAATGGAAGA  >  NC_000913/389565‑389830                                                                                                                                       |                                                                                                                                    gcATCACGCCTGGCATGGCTTCAACGGCTTTGTAGTCGTCAATTTCTTCTTCAACAAAGATCGGCAACACCAGGTCGTTAAGGCTAAGTGTTGTCTCTTCAAACATAGCGCGCAGCGCAGGAGATTTGCGCAGGagacga                                                                                                                                >  1:44720/1‑140 (MQ=255)   aTCACGCCTGGCATGGCTTCAACGGCTTTGTAGTCGTCAATTTCTTCTTCAACAAAGATCGGCAACACCAGGTCGTTAAGGCTAAGTGTTGTCTCTTCAAACATAGCGCGCAGCGCAGGAGATTTGCGCAGGAGACGAgg                                                                                                                              >  1:111576/1‑140 (MQ=255)     cACGCCTGGCATGGCTTCAACGGCTTTGTAGTCGTCAATTTCTTCTTCAACAAAGATCAGCAACACCAGGTCGTTAAGGCTAAGTGTTGTCTCTTCAAACATAGCGCGCAGCGCAGGAGATTTGCGCAGGAGACGAGGGc                                                                                                                            >  1:816978/1‑140 (MQ=255)      aCGCCTGGCATGGCTTCAACGGCTTTGTAGTCGTCAATTTCTTCTTCAACAAAGATCAGCAACACCAGGTCGTTAAGGCTAAGTGTTGTCTCTTCAAACATAGCGCGCAGCGCAGGAGATTTGCGCAGGAGACGAGGGCg                                                                                                                           <  2:816978/140‑1 (MQ=255)       cGCCTGGCATGGCTTCAACGGCTTTGTAGTCGTCAATTTCTTCTTCAACAAAGATCGGCAACACCAGGTCGTTAAGGCTAAGTGTTGTCTCTTCAAACATAGCGCGCAGCGCAGGAGATTTGCGCAGGAGACGAGGGCGt                                                                                                                          <  2:623395/140‑1 (MQ=255)       cGCCTGGCATGGCTTCAACGGCTTTGTAGTCGTCAATTTCTTCTTCAACAAAGATCGGCAACACCAGGTCGTTAAGGCTAAGTGTTGTCTCTTCAAACATAGCGCGCAGCGCAGGAGATTTGCGCAGGAGACGAGGGCGt                                                                                                                          <  2:779709/140‑1 (MQ=255)             gCATGGCTTCAACGGCTTTGTAGTCGTCAATTTCTTCTTCAACAAAGATCGGCAACACCAGGTCGTTAAGGCTAAGTGTTGTCTCTTCAAACATAGCGCGCAGCGCAGGAGATTTGCGCAGGAGACGAGGGCGTTGGAtt                                                                                                                    <  2:44720/140‑1 (MQ=255)                 ggCTTCAACGGCTTTGTAGTCGTCAATTTCTTCTTCAACAAAGATCGGCAACACCAGGTCGTTAAGGCTAAGTGTTGTCTCTTCAAACATAGCGCGCAGCGCAGGAGATTTGCGCAGGAGACGAGGGCGTTGGATTAAGt                                                                                                                <  1:581191/140‑1 (MQ=255)                     tCAACGGCTTTGTAGTCGTCAATTTCTTCTTCAACAAAGATCGGCAACACCAGGTCGTTAAGGCTAAGTGTTGTCTCTTCAAACATAGCGCGCAGCGCAGGAGATTTGCGCAGGAGACGAGGGCGTTGGATTAAGTCTGt                                                                                                            <  2:297643/140‑1 (MQ=255)                       aaCGGCTTTGTAGTCGTCAATTTCTTCTTCAACAAAGATCGGCAACACCAGGTCGTTAAGGCTAAGTGTTGTCTCTTCAAACATAGCGCGCAGCGCAGGAGATTTGCGCAGGAGACGAGGGCGTTGGATTAAGTCTGTCa                                                                                                          >  1:634576/1‑140 (MQ=255)                       aaCGGCTTTGTAGTCGTCAATTTCTTCTTCAACAAAGATCGGCAACACCAGGTCGTTAAGGCTAAGTGTTGTCTCTTCAAACATAGCGCGCAGCGCAGGAGATTTGCGCAGGAGACGAGGGCGTTGGATTAAGTCTGTCa                                                                                                          <  2:786512/140‑1 (MQ=255)                          ggCTTTGTAGTCGTCAATTTCTTCTTCAACAAAGATCGGCAACACCAGGTCGTTAAGGCTAAGTGTTGTCTCTTCAAACATAGCGCGCAGCGCAGGAGATTTGCGCAGGAGACGAGGGCGTTGGATTAAGTCTGTCATgg                                                                                                       <  1:318464/140‑1 (MQ=255)                             tttGTAGTCGTCAATTTCTTCTTCAACAAAGATCGGCAACACCAGGTCGTTAAGGCTCAGCGTTGTCTCTTCAAACATAGCGCGCAGCGCAGGAGATTTGCGCAGGAGACGAGGGCGTTGGATTAAGTCTGTCATGGTCt                                                                                                    >  2:140706/1‑140 (MQ=255)                             tttGTAGTCGTCAATTTCTTCTTCAACAAAGATCGGCAACACCAGGTCGTTAAGGCTAAGTGTTGTCTCTTCAAACATAGCGCGCAGCGCAGGAGATTTGCGCAGGAGACGAGGGCGTTGGATTAAGTCTGTCATGGTCt                                                                                                    >  2:697338/1‑140 (MQ=255)                                      gtcAATTTCTTCTTCAACAAAGATCGGCAACACCAGGTCGTTAAGGCTAAGTGTTGTCTCTTCAAACATAGCGCGCAGCGCAGGAGATTTGCGCAGGAGACGAGGGCGTTGGATTAAGTCTGTCATGGTCTGCCTGATGt                                                                                           >  1:159767/1‑140 (MQ=255)                                      gtcAATTTCTTCTTCAACAAAGATCGGCAACACCAGGTCGTTAAGGCTAAGTGTTGTCTCTTCAAACATAGCGCGCAGCGCAGGAGATTTGCGCAGGAGACGAGGGCGTTGGATTAAGTCTGTCATGGTCTGCCTGATGt                                                                                           >  2:749065/1‑140 (MQ=255)                                      gtcAATTTCTTCTTCAACAAAGATCGGCAACACCAGGTCGTTAAGGCTAAGTGTTGTCTCTTCAAACATAGCGCGCAGCGCAGGAGATTTGCGCAGGAGACGAGGGCGTTGGATTAAGTCTGTCATGGTCTGCCTGATGt                                                                                           >  1:66134/1‑140 (MQ=255)                                        cAATTTCTTCTTCAACAAAGATCGGCAACACCAGGTCGTTAAGGCTAAGTGTTGTCTCTTCAAACATAGCGCGCAGCGCAGGAGATTTGCGCAGGAGACGAGGGCGTTGGATTAAGTCTGTCATGGTCTGCCTGATGttt                                                                                         <  2:736268/140‑1 (MQ=255)                                        cAATTTCTTCTTCAACAAAGATCGGCAACACCAGGTCGTTAAGGCTAAGTGTTGTCTCTTCAAACATAGCGCGCAGCGCAGGAGATTTGCGCAGGAGACGAGGGCGTTGGATTAAGTCTGTCATGGTCTGCCTGATGttt                                                                                         >  2:328036/1‑140 (MQ=255)                                                   tcAACAAAGATCGGCAACACCAGGTCGTTAAGGCTAAGTGTTGTCTCTTCAAACATAGCGCGCAGCGCAGGAGATTTGCGCAGGAGACGAGGGCGTTGGATTAAGTCTGTCATGGTCTGCCTGATGTTTGTGGAATCGAg                                                                              <  1:176054/140‑1 (MQ=255)                                                      acaaAGATCGGCAACACCAGGTCGTTAAGGCTAAGTGTTGTCTCTTCAAACATAGCGCGCAGCGCAGGAGATTTGCGCAGGAGACGAGGGCGTTGGATTAAGTCTGTCATGGTCTGCCTGATGTTTGTGGAATCGAgggg                                                                           <  2:66134/140‑1 (MQ=255)                                                       caaAGATCGGCAACACCAGGTCGTTAAGGCTAAGTGTTGTCTCTTCAAACATAGCGCGCAGCGCAGGAGATTTGCGCAGGAGACGAGGGCGTTGGATTAAGTCTGTCATGGTCTGCCTGATGTTTGTGGAATCGAGGGGc                                                                          >  1:244560/1‑140 (MQ=255)                                                            aTCGGCAACACCAGGTCGTTAAGGCTAAGTGTTGTCTCTTCAAACATAGCGCGCAGCGCAGGAGATTTGCGCAGGAGACGAGGGCGTTGGATTAAGTCTGTCATGGTCTGCCTGATGTTTGTGGAATCGAGGGGCATAGt                                                                     <  1:749065/140‑1 (MQ=255)                                                                 cAACACCAGGTCGTTAAGGCTAAGTGTTGTCTCTTCAAACATAGCGCGCAGCGCAGGAGATTTGCGCAGGAGACGAGGGCGTTGGATTAAGTCTGTCATGGTCTGCCTGATGTTTGTGGAATCGAg                                                                              <  1:220821/126‑1 (MQ=255)                                                                 cAACACCAGGTCGTTAAGGCTAAGTGTTGTCTCTTCAAACATAGCGCGCAGCGCAGGAGATTTGCGCAGGAGACGAGGGCGTTGGATTAAGTCTGTCATGGTCTGCCTGATGTTTGTGGAATCGAg                                                                              >  2:220821/1‑126 (MQ=255)                                                                      ccAGGTCGTTAAGGCTAAGTGTTGTCTCTTCAAACATAGCGCGCAGCGCAGGAGATTTGCGCAGGAGACGAGGGCGTTGGATTAAGTCTGTCATGGTCTGCCTGATGTTTGTGGAATCGAGGGGCATAGTATACCTGAAg                                                           <  1:697338/140‑1 (MQ=255)                                                                        aGGTCGTTAAGGCTAAGTGTTGGCTCTTCAAACATAGCGCGCAGCGCAGGAGATTTGCGCAGGAGACGAGGGCGTTGGATTAAGTCTGTCATGGTCTGCCTGATGTTTGTGGAATCGAGGGGCATAGTATACCTGAAGCa                                                         <  1:140706/140‑1 (MQ=255)                                                                                   gCTAAGTGTTGTCTCTTCAAACATAGCGCGCAGCGCAGGAGATTTGCGCAGGAGACGAGGGCGTTGGATTAAGTCTGTAATGGTCTGCCTGATGTTTGTGGAATCGAGGGGCATAGTATACCTGAAGCAGGGTAGGGATg                                              >  2:626515/1‑140 (MQ=255)                                                                                       aGTGTTGTCTCTTCAAACATAGCGCGCAGCGCAGGAGATTTGCGCAGGAGACGAGGGCGTTGGATTAAGTCTGTCATGGTCTGCCTGATGTTTGTGGAATCGAGGGGCATAGTATACCTGAAGCAGGGTAGGGATGtttt                                          <  2:244560/140‑1 (MQ=255)                                                                                       aGTGTTGTCTCTTCAAACATAGCGCGCAGCGCAGGAGATTTGCGCAGGAGACGAGGGCGTTGGATTAAGTCTGTCATGGTCTGCCTGATGTTTGTGGAATCGAGGGGCATAGTATACCTGAAGCAGGGTAGGGATGtttt                                          >  1:502908/1‑140 (MQ=255)                                                                                       aGTGTTGTCTCTTCAAACATAGCGCGCAGCGCAGGAGATTTGCGCAGGAGACGAGGGCGTTGGATTAAGTCTGTCATGGTCTGCCTGATGTTTGTGGAATCGAGGGGCATAGTATACCTGAAGCAGGGTAGGGATGtttt                                          >  2:418151/1‑140 (MQ=255)                                                                                        gtgtTGTCTCTTCAAACATAGCGCGCAGCGCAGGAGATTTGCGCAGGAGACGAGGGCGTTGGATCAAGTCTGTCATGGTCTGCCTGATGTTTGTGGAATCGAGGGGCATAGTATACCTGAAGCAGGGTAGGGATGTTTTa                                         >  1:689024/1‑140 (MQ=255)                                                                                             gtCTCTTCAAACATAGCGCGCAGCGCAGGAGATTTGCGCAGGAGACGAGGGCGTTGGATTAAGTCTGTCATGGTCTGCCTGATGTTTGTGGAATCGAGGGGCATAGTATACCTGAAGCAGGGTAGGGATGTTTTACGaaa                                    >  2:441526/1‑140 (MQ=255)                                                                                             gtCTCTTCAAACATAGCGCGCAGCGCAGGAGATTTGCGCAGGAGACGAGGGCGTTGGATTAAGTCTGTCATGGTCTGCCTGATGTTTGTGGAATCGAGGGGCATAGTATACCTGAAGCAGGGTAGGGATGTTTTACGaaa                                    >  2:445680/1‑140 (MQ=255)                                                                                              tctcTTCAAACATAGCGCGCAGCGCAGGAGATTTGCGCAGGAGACGAGGGCGTTGGATTAAGTCTGTCATGGTCTGCCTGATGTTTGTGGAATCGAGGGGCATAGTATACCTGAAGCAGGGTAGGGATGTTTTACGAAAg                                   <  2:361906/140‑1 (MQ=255)                                                                                                  ttCAAACATAGCGCGCAGCGCAGGAGATTTGCGCAGGAGACGAGGGCGTTGGATTAAGTCTGTCATGGTCTGCCTGATGTTTGTGGAATCGAGGGGCATAGTATACCTGAAGCAGGGTAGGGATGTTTTACGAAAGTTGt                               >  1:343167/1‑140 (MQ=255)                                                                                                  ttCAAACATAGCGCGCAGCGCAGGAGATTTGCGCAGGAGACGAGGGCGTTGGATTAAGTCTGTCATGGTCTGCCTGATGTTTGTGGAATCGAGGGGCATAGTATACCTGAAGCAGGGTAGGGATGTTTTACGAAAGTTGt                               >  2:810500/1‑140 (MQ=255)                                                                                                    cAAACATAGCGCGCAGCGCAGGAGATTTGCGCAGGAGACGAGGGCGTTGGATTAAGTCTGTCATGGTCTGCCTGATGTTTGTGGAATCGAGGGGCATAGTATACCTGAAGCAGGGTAGGGATGTTTTACGAAAGTTGTcg                             <  1:328036/140‑1 (MQ=255)                                                                                                           aGCGCGCAGCGCAGGAGATTTGCGCAGGAGACGAGGGCGTTGGATTAAGTCTGTCATGGTCTGCCTGATGTTTGTGGAATCGAGGGGCATAGTATACCTGAAGCAGGGTAGGGATGTTTTACGAAAGTTGTCGCGATGtt                      >  1:566664/1‑140 (MQ=255)                                                                                                            gcgcgcAGCGCAGGAGATTTGCGCAGGAGACGAGGGCGTTGGATTAAGTCTGTCATGGTCTGCCTGATGTTTGTGGAATCGAGGGGCATAGTATACCTGAAGCAGGGTAGGGATGTTTTACGAAAGTTGTcg                             <  2:133048/132‑1 (MQ=255)                                                                                                            gcgcgcAGCGCAGGAGATTTGCGCAGGAGACGAGGGCGTTGGATTAAGTCTGTCATGGTCTGCCTGATGTTTGTGGAATCGAGGGGCATAGTATACCTGAAGCAGGGTAGGGATGTTTTACGAAAGTTGTcg                             >  1:133048/1‑132 (MQ=255)                                                                                                                  agcgcagGAGATTTGCGCAGGAGACGAGGGCGTTGGATTAAGTCTGTCATGGTCTGCCTGATGTTTGTGGAATCGAGGGGCATAGTATACCTGAAGCAGGGTAGGGATGTTTTACGAAAGTTGTCGCGATGTTGACaaga               >  2:11877/1‑140 (MQ=255)                                                                                                                   gcgcagGAGATTTGCGCAGGAGACGAGGGCGTTGGATTAAGTCTGTCATGGTCTGCCTGATGTTTGTGGAATCGAGGGGCATAGTATACCTGAAGCAGGGTAGGGATGTTTTACGAAAGTTGTCGCGATGTTGACaagaa              <  2:502908/140‑1 (MQ=255)                                                                                                                               tGCGCAGGAGACGAGGGCGTTGGATTAAGTCTGTCATGGTCTGCCTGATGTTTGTGGAATCGAGGGGCATAGTATACCTGAAGCAGGGTAGGGATGTTTTACGAAAGTTGTCGCGATGTTGACAAGAAGAGAATGGAaga  <  2:343167/140‑1 (MQ=255)                                                                                                                               tGCGCAGGAGACGAGGGCGTTGGATTAAGTCTGTCATGGTCTGCCTGATGTTTGTGGAATCGAGGGGCATAGTATACCTGAAGCAGGGTAGGGATGTTTTACGAAAGTTGTCGCGATGTTGACAAGAAGAGAATGGAaga  <  1:11877/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                    GCATCACGCCTGGCATGGCTTCAACGGCTTTGTAGTCGTCAATTTCTTCTTCAACAAAGATCGGCAACACCAGGTCGTTAAGGCTAAGTGTTGTCTCTTCAAACATAGCGCGCAGCGCAGGAGATTTGCGCAGGCGACGAGGGCGTTGGATTAAGTCTGTCATGGTCTGCCTGATGTTTGTGGAATCGAGGGGCATAGTATACCTGAAGCAGGGTAGGGATGTTTTACGAAAGTTGTCGCGATGTTGACAAGAAGAGAATGGAAGA  >  NC_000913/389565‑389830

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑