Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,367,748	C→A	D194Y (GAC→TAC)	aspA ←	aspartate ammonia‑lyase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,367,748	0	C	A	100.0%	209.9 / NA	60	D194Y (GAC→TAC)	aspA	aspartate ammonia‑lyase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (31/29);  total (31/29)

CAGTACCGATTGCTGTTGCACCAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTCCTGCAGCTGGGTACGACCCATTTTCAGGATGTCCTGGAATTCGACAGCTTTACGTTCAAAGCCTTCACGCAGTTGGTTAATCGCATCTACCAGCTTAATCAGGGAAGAGTAAACTGCGATACGGAAACCGGTCGGG  >  NC_000913/4367616‑4367884                                                                                                                                     |                                                                                                                                         cAGTACCGATTGCTGTTGCACCAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTACTGCAGc                                                                                                                                   <  2:779046/140‑1 (MQ=255)    tACCGATTGCTGTTGCACCAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTACTGCAGCTgg                                                                                                                                >  2:755694/1‑140 (MQ=255)    tACCGATTGCTGTTGCACCAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTACTGCAGCTgg                                                                                                                                >  2:679993/1‑140 (MQ=255)    tACCGATTGCTGTTGCACCAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTACTGCAGCTgg                                                                                                                                >  2:186816/1‑140 (MQ=255)           tGCTGTTGCACCAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTACTGCAGCTGGGTACGAc                                                                                                                         <  1:755694/140‑1 (MQ=255)           tGCTGTTGCACCAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTACTGCAGCTGGGTACGAc                                                                                                                         >  2:148067/1‑140 (MQ=255)            gCTGTTGCACCAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTACTGCAg                                                                                                                                    <  1:596667/128‑1 (MQ=255)            gCTGTTGCACCAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTACTGCAg                                                                                                                                    >  2:596667/1‑128 (MQ=255)            gCTGTTGCACCAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTACTGCAGCTGGGTACGAcc                                                                                                                        <  2:870282/140‑1 (MQ=255)              tGTTGCACCAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTACTGCAGCTGGGTACGACCCa                                                                                                                      >  2:246950/1‑140 (MQ=255)                ttGCACCAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTACTGCAGCTGGGTACGACCCAtt                                                                                                                    >  2:317571/1‑140 (MQ=255)                     ccAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTACTGCAGCTGGGTACGAcc                                                                                                                        >  2:607985/1‑131 (MQ=255)                     ccAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTACTGCAGCTGGGTACGAcc                                                                                                                        <  1:607985/131‑1 (MQ=255)                      cAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTACTGCAGCTGGGTACGACCCATTTTCAgg                                                                                                              <  2:58434/140‑1 (MQ=255)                      cAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTACTGCAGCTGGGTACGACCCATTTTCAgg                                                                                                              <  1:317571/140‑1 (MQ=255)                        aGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTACTGCAGCTGGGTACGACCCATTTTCAGGAt                                                                                                            >  2:266276/1‑140 (MQ=255)                             aaCTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTACTGCAGCTGGGTACGACCCATTTTCAgg                                                                                                              >  2:61019/1‑133 (MQ=255)                             aaCTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTACTGCAGCTGGGTACGACCCATTTTCAgg                                                                                                              <  1:61019/133‑1 (MQ=255)                              aCTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTTACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTACTGCAGCTGGGTACGACCCATTTTCAGGATGTCCTg                                                                                                      >  1:321439/1‑140 (MQ=255)                                  ccAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTACTGCAGCTGGGTACGACCCATTTTCAGGATGTCCTGGAAt                                                                                                  >  1:858064/1‑140 (MQ=255)                                    agcagcagTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTACTGCAGCTGGGTACGACCCATTTTCAGGATGTCCTGGAATTc                                                                                                >  2:117340/1‑140 (MQ=255)                                    agcagcagTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTACTGCAGCTGGGTACGACCCATTTTCAGGATGTCCTGGAATTc                                                                                                >  2:126909/1‑140 (MQ=255)                                              cAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTACTGCAGCTGGGTACGACCCATTTTCAGGATGTCCTGGAATTCGACAGCTTTa                                                                                      <  2:261506/140‑1 (MQ=255)                                                     aCGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTACTGCAGCTGGGTACGACCCATTTTCAGGATGTCCTGGAATTCGACAGCTTTACGTTCaa                                                                               <  1:126909/140‑1 (MQ=255)                                                           gATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTACTGCAGCTGGGTACGACCCATTTTCAGGATGTCCTGGAATTCGACAGCTTTTTGTTCAAAGCCtt                                                                         <  2:154421/140‑1 (MQ=255)                                                                ttttCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTACTGCAGCTGGGTACGACCCATTTTCAGGATGTCCTGGAATTCGACAGCTTTACGTTCAAAGCCTTCACGc                                                                    <  1:679993/140‑1 (MQ=255)                                                                ttttCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTACTGCAGCTGGGTACGACCCATTTTCAGGATGTCCTGGAATTCGACAGCTTTACGTTCAAAGCCTTCACGc                                                                    <  1:186816/140‑1 (MQ=255)                                                                ttttCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTACTGCAGCTGGGTACGACCCATTTTCAGGATGTCCTGGAATTCGACAGCTTTACGTTCAAAGCCTTCACGc                                                                    <  1:296933/140‑1 (MQ=255)                                                                 ttttACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTACTGCAGCTGGGTACGACCCATTTTCAGGATGTCCTGGAATTCGACAGCTTTACGTTCAAAGCCTTCACGCa                                                                   <  2:321439/140‑1 (MQ=255)                                                                 tttCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTACTGCAGCTGGGTACGACCCATTTTCAGGATGTCCTGGAATTCGACAGCTTTACGTTCAAAGCCTTCACGCa                                                                   >  2:222460/1‑140 (MQ=255)                                                                  ttCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTACTGCAGCTGGGTACGACCCATTTTCAGGATGTCCTGGAATTCGACAGCTTTACGTTCAAAGCCTTCACGCAg                                                                  >  2:106351/1‑140 (MQ=255)                                                                     aCTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTACTGCAGCTGGGTACGACCCATTTTCAGGATGTCCTGGAATTCGACAGCTTTACGTTCAAAGCCTTCACGCAGTTg                                                               >  1:277587/1‑140 (MQ=255)                                                                       ttcttcttTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTACTGCAGCTGGGTACGACCCATTTTCAGGATGTCCTGGAATTCGACAGCTTTACGTTCAAAGCATTCa                                                                       <  1:559922/130‑1 (MQ=255)                                                                       ttcttcttTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTACTGCAGCTGGGTACGACCCATTTTCAGGATGTCCTGGAATTCGACAGCTTTACGTTCAAAGCATTCa                                                                       >  2:559922/1‑130 (MQ=255)                                                                           tcttTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTACTGCAGCTGGGTACGACCCATTTTCAGGATGTCCTGGAATTCGACAGCTTTACGTTCAAAGCCTTCACGCAGTTGGTTAAt                                                         >  1:536022/1‑140 (MQ=255)                                                                               tCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTACTGCAGCTGGGTACGACCCATTTTCAGGATGTCCTGGAATTCGACAGCTTTACGTTCAAAGCCTTCACGCAGTTGGTTAATCGCa                                                     <  1:246950/140‑1 (MQ=255)                                                                                   cagGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTACTGCAGCTGGGTACGACCCATTTTCAGGATGTCCTGGAATTCGACAGCTTTACGTTCAAAGCCTTCACGCAGTTGGTTAATCGCATCTa                                                 >  2:776840/1‑140 (MQ=255)                                                                                   cagGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTACTGCAGCTGGGTACGACCCATTTTCAGGATGTCCTGGAATTCGACAGCTTTACGTTCAAAGCCTTCACGCAGTTGGTTAATCGCATCTa                                                 <  1:776840/140‑1 (MQ=255)                                                                                   cagGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTACTGCAGCTGGGTACGACCCATTTTCAGGATGTCCTGGAATTCGACAGCTTTACGTTCAAAGCCTTCACGCAGTTGGTTAATCGCATCTa                                                 <  2:536022/140‑1 (MQ=255)                                                                                      gATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTACTGCAGCTGGGTACGACCCATTTTCAGGATGTCCTGGAATTCGACAGCTTTACGTTCAAAGCCTTCACGCAGTTGGTTAATCGCATCTACCa                                              >  1:793539/1‑140 (MQ=255)                                                                                      gATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTACTGCAGCTGGGTACGACCCATTTTCAGGATGTCCTGGAATTCGACAGCTTTACGTTCAAAGCCTTCACGCAGTTGGTTAATCGCATCTACCa                                              >  2:423791/1‑140 (MQ=255)                                                                                        tGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTACTGCAGCTGGGTACGACCCATTTTCAGGATGTCCTGGAATTCGACAGCTTTACGTTCAAAGCCTTCACGCAGTTGGTTAATCGCATCTACCAGc                                            <  1:74265/140‑1 (MQ=255)                                                                                        tGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTACTGCAGCTGGGTACGACCCATTTTCAGGATGTCCTGGAATTCGACAGCTTTACGTTCAAAGCCTTCACGCAGTTGGTTAATCGCATCTACCAGc                                            <  1:222460/140‑1 (MQ=255)                                                                                        tGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTACTGCAGCTGGGTACGACCCATTTTCAGGATGTCCTGGAATTCGACAGCTTTACGTTCAAAGCCTTCACGCAGTTGGTTAATCGCATCTACCAGc                                            <  1:308578/140‑1 (MQ=255)                                                                                         gCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTACTGCAGCTGGGTACGACCCATTTTCAGGATGTCCTGGAATTCGACAGCTTTACGTTCAAAGCCTTCACGCAGTTGGTTAATCGCATCTa                                                 >  1:780591/1‑134 (MQ=255)                                                                                         gCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTACTGCAGCTGGGTACGACCCATTTTCAGGATGTCCTGGAATTCGACAGCTTTACGTTCAAAGCCTTCACGCAGTTGGTTAATCGCATCTa                                                 <  2:780591/134‑1 (MQ=255)                                                                                             aaaGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTACTGCAGCTGGGTACGACCCATTTTCAGGATGTCCTGGAATTCGACAGCTTTACGTTCAAAGCCTTCACGCAGTTGGTTAATCGCATCTACCAGCTTAAt                                       >  2:219184/1‑140 (MQ=255)                                                                                               aGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTACTGCAGCTGGGTACGACCCATTTTCAGGATGTCCTGGAATTCGACAGCTTTACGTTCAAAGCCTTCACGCAGTTGGTTAATCGCATCTACCAGCTTAATCa                                     <  1:370615/140‑1 (MQ=255)                                                                                                gcgcGGAATTCCTGACCGAGAGTCATCGGTACTGCGTACTGCAGCTGGGTACGACCCATTTTCAGGATGTCCTGGAATTCGACAGCTTTACGTTCAAAGCCTTCACGCAGTTGGTTAATCGc                                                      <  1:614588/122‑1 (MQ=255)                                                                                                gcgcGGAATTCCTGACCGAGAGTCATCGGTACTGCGTACTGCAGCTGGGTACGACCCATTTTCAGGATGTCCTGGAATTCGACAGCTTTACGTTCAAAGCCTTCACGCAGTTGGTTAATCGc                                                      >  2:614588/1‑122 (MQ=255)                                                                                                   cgGAATTCCTGACCGAGGGTCATCGGTACTGCGTACTGCAGCTGGGTACGACCCATTTCCAGGATGTCCTGGAATTCGACAGCTTTACGTTCAAAGCCTTCACGCAGTTGGTTAATCGCATCTACCAGCTTAATCAGGGa                                 >  2:43600/1‑140 (MQ=255)                                                                                                      aaTTCCTGACCGAGGGTCATCGGTACTGCGTACTGCAGCTGGGTACGACCCATTTTCAGGATGTCCTGGAATTCGACAGCTTTACGTTCAAAGCCTTCACGCAGTTGGTTAATCGCATCTCCCAGCTTAATCAGGGAaga                              <  2:793539/140‑1 (MQ=255)                                                                                                                cGAGGGTCATCGGTACTGCGTACTGCAGCTGGGTACGACCCATTTTCAGGATGTCCTGGAATTCGACAGCTTTACGTTCAAAGCCTTCACGCAGTTGGTTAATCGCATCTACCAGCTTAATCAGGGAAGAGTAAACTGCg                    <  2:796347/140‑1 (MQ=255)                                                                                                                  aGGGTCATCGGTACTGCGTACTGCAGCTGGGTACGACCCATTTTCAGGATGTCCTGGAATTCGACAGCTTTACGTTCAAAGCCTTCACGCAGTTGGTTAATCGCATCTACCAGCTTAATCAGGGAAGAGTAAACTGCGAt                  >  1:575745/1‑140 (MQ=255)                                                                                                                        aTCGGTACTGCGTACTGCAGCTGGGTACGACCCATTTTCAGGATGTCCTGGAATTCGACAGCTTTACGTTCAAAGCCTTCACGCAGTTGGTTAATCGCATCTACCAGCTTAATCAGGGAAGAGTAAACTGCGATACGGaa            >  2:658038/1‑140 (MQ=255)                                                                                                                        aTCGGTACTGCGTACTGCAGCTGGGTACGACCCATTTTCAGGATGTCCTGGAATTCGACAGCTTTACGTTCAAAGCCTTCACGCAGTTGGTTAATCGCATCTACCAGCTCAATCAGGGAAGAGTAAACTGCGATACGGaa            >  2:855268/1‑140 (MQ=255)                                                                                                                              aCTGCGTACTGCAGCTGGGTACGACCCATTTTCAGGATGTCCTGGAATTCGACAGCTTTACGTTCAAAGCCTTCACGCAGTTGGTTAATCGCATCTACCAGCTTAATCAGGGAAGAGTAAACTGCGATACGGAAACCGGt      <  1:658038/140‑1 (MQ=255)                                                                                                                               cTGCGTACTGCAGCTGGGTACGACCCATTTTCAGGATGTCCTGGAATTCGACAGCTTTACGTTCAAAGCCTTCACGCAGTTGGTTAATCGCATCTACCAGCTTAATCAGGGAAGAGTAAACTGCGATACGGAAACCGGTc     >  1:586539/1‑140 (MQ=255)                                                                                                                               cTGCGTACTGCAGCTGGGTACGACCCATTTTCAGGATGTCCTGGAATTCGACAGCTTTACGTTCAAAGCCTTCACGCAGTTGGTTAATCGCATCTACCAGCTTAATCAGGGAAGAGTAAACTGCGATACGGAAACCGGTc     >  2:312107/1‑140 (MQ=255)                                                                                                                               cTGCGTACTGCAGCTGGGTACGACCCATTTTCAGGATGTCCTGGAATTCGACAGCTTTACGTTCAAAGCCTTCACGCAGTTGGTTAATCGCATCTACCAGCTTAATCAGGGAAGAGTAAACTGCGATACGGAAACCGGTc     <  1:423791/140‑1 (MQ=255)                                                                                                                                  cGTACTGCAGCTGGGTACGACCCATTTTCAGGATGTCCTGGAATTCGACAGCTTTACGTTCAAAGCCTTCACGCAGTTGGTTAATCGCATCTACCAGCTTAATCAGGGAAGAGTAAACTGCGATACGGAAACCGGTCggg  <  1:219184/140‑1 (MQ=255)                                                                                                                                     |                                                                                                                                         CAGTACCGATTGCTGTTGCACCAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTCCTGCAGCTGGGTACGACCCATTTTCAGGATGTCCTGGAATTCGACAGCTTTACGTTCAAAGCCTTCACGCAGTTGGTTAATCGCATCTACCAGCTTAATCAGGGAAGAGTAAACTGCGATACGGAAACCGGTCGGG  >  NC_000913/4367616‑4367884

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑