Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	855,922	C→A	intergenic (‑178/‑41)	ldtB ← / → ybiT	L,D‑transpeptidase YbiS/ABC transporter ATP‑binding protein YbiT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	855,922	0	C	A	100.0%	187.2 / NA	53	intergenic (‑178/‑41)	ldtB/ybiT	L,D‑transpeptidase YbiS/ABC transporter ATP‑binding protein YbiT
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (29/24);  total (29/24)

TTAGAGTATTGGAAGCGCATTATAAGTTCATTCCAGCTCACAGTGAAATCAGATGTGTACGAAATCACATTTTTTGCCTTTGGCTTGAGTGTAGACCTTAAGCGAGGAGCAGGATCTTCTTTCAGACTTATGGCATAATGCGCGGTTTGTCATATCTCTTTTCAGGATACGCCTGTGTTAGTTTCCAGTAACGTCACCATGCAGTTCGGCAGTAAGCCGTTGTTTGAAAACATTTCCGTCAAATTTGGCGGCGGCAACCGTTACGGCCT  >  NC_000913/855789‑856057                                                                                                                                      |                                                                                                                                        ttAGAGTATTGGAAGCGCATTATAAGTTCATTCCAGCTCACAGTGAAATCAGATGTGTACGAAATCACATTTTTTGCCTTTGGCTTGAGTGTAGACCTTAAGCGAGGAGCAGGATCTTCTTTCAGACTTATGGAATAATg                                                                                                                                   <  2:813262/140‑1 (MQ=255)    gagTATTGGAAGCGCATTATAAGTTCATTCCAGCTCACAGTGAAATCAGATGTGTACGAAATCACATTTTTTGCCTTTGGCTTGAGTGTAGACCTTAAGCGAGGAGCAGGATCTTCTTTCAGACTTATGGAATAATgcgc                                                                                                                                <  1:45984/140‑1 (MQ=255)     agTATTGGAAGCGCATTATAAGTTCATTCCAGCTCACAGTGAAATCAGATGTGTACGAAATCACATTTTTTGCCTTTGGCTTGAGTGTAGACCTTAAGCGAGGAGCAGGATCTTCTTTCAGACTTATGGAATAATGcgcg                                                                                                                               >  1:211102/1‑140 (MQ=255)     agTATTGGAAGCGCATTATAAGTTCATTCCAGCTCACAGTGAAATCAGATGTGTACGAAATCACATTTTTTGCCTTTGGCTTGAGTGTAGACCTTAAGCGAGGAGCAGGATCTTCTTTCAGACTTATGGAATAATGcgcg                                                                                                                               <  2:302693/140‑1 (MQ=255)     agTATTGGAAGCGCATTATAAGTTCATTCCAGCTCACAGTGAAATCAGATGTGTACGAAATCACATTTTTTGCCTTTGGCTTGAGTGTAGACCTTAAGCGAGGAGCAGGATCTTCTTTCAGACTTATGGAATAATGcgcg                                                                                                                               <  1:883979/140‑1 (MQ=255)         ttGGAAGCGCATTATAAGTTCATTCCAGCTCACAGTGAAATCAGATGTGTACGAAATCACATTTTTTGCCTTTGGCTTGAGTGTAGACCTTAAGCGAGGAGCAGGATCTTCTTTCAGACTTATGGAATAATGCGCGGttt                                                                                                                           >  1:167644/1‑140 (MQ=255)         ttGGAAGCGCATTATAAGTTCATTCCAGCTCACAGTGAAATCAGATGTGTACGAAATCACATTTTTTGCCTTTGGCTTGAGTGTAGACCTTAAGCGAGGAGCAGGATCTTCTTTCAGACTTATGGAATAATGCGCGAttt                                                                                                                           >  1:305157/1‑140 (MQ=255)          tGGAAGCGCATTATAAGTTCATTCCAGCTCACAGTGAAATCAGATGTGTACGAAATCACATTTTTTGCCTTTGGCTTGAGTGTAGACCTTAAGCGAGGAGCAGGATCTTCTTTCAGACTTATGGAATAATGCGCGGTTTg                                                                                                                          >  1:776574/1‑140 (MQ=255)                cgcATTATAAGTTCATTCCAGCTCACAGTGAAATCAGATGTGTACGAAATCACATTTTTTGCCTTTGGCTTGAGTGTAGACCTTAAGCGAGGAGCAGGATCTTCTTTCAGACTTATGGAATAATGCGCGGTTTGTCatat                                                                                                                    >  1:238473/1‑140 (MQ=255)                cgcATTATAAGTTCATTCCAGCTCACAGTGAAATCAGATGTGTACGAAATCACATTTTTTGCCTTTGGCTTGAGTGTAGACCTTAAGCGAGGAGCAGGATCTTCTTTCAGACTTATGGAATAATGCGCGGTTTGTCatat                                                                                                                    <  2:211102/140‑1 (MQ=255)                 gcATTATAAGTTCATTCCAGCTCACAGTGAAATCAGATGTGTACGAAATCACATTTTTTGCCTTTGGCTTGAGTGTAGACCTTAAGCGAGGAGCAGGATCTTCTTTCAGACTTATGGAATAATGCGCGGTTTGTCATAtc                                                                                                                   >  1:374014/1‑140 (MQ=255)                  cATTATAAGTTCATTCCAGCTCACAGTGAAATCAGATGTGTACGAAATCACATTTTTTGCCTTTGGCTTGAGTGTAGACCTTAAGCGAGGAGCAGGATCTTCTTTCAGACTTATGGAATAATGcgcg                                                                                                                               <  2:551857/127‑1 (MQ=255)                  cATTATAAGTTCATTCCAGCTCACAGTGAAATCAGATGTGTACGAAATCACATTTTTTGCCTTTGGCTTGAGTGTAGACCTTAAGCGAGGAGCAGGATCTTCTTTCAGACTTATGGAATAATGcgcg                                                                                                                               >  1:551857/1‑127 (MQ=255)                  cATTATAAGTTCATTCCAGCTCACAGTGAAATCAGATGTGTACGAAATCACATTTTTTGCCTTTGGCTTGAGTGTAGACCTTAAGCGAGGAGCAGGATCTTCTTTCAGACTTATGGAATAATGCGCGGTTTGTCATAtct                                                                                                                  <  2:777192/140‑1 (MQ=255)                  cATTATAAGTTCATTCCAGCTCACAGTGAAATCAGATGTGTACGAAATCACATTTTTTGCCTTTGGCTTGAGTGTAGACCTTAAGCGAGGAGCAGGATCTTCTTTCAGACTTATGGAATAATGCGCGGTTTGTCATAtct                                                                                                                  <  1:804009/140‑1 (MQ=255)                       taAGTTCATTCCAGCTCACAGTGAAATCAGATGTGTACGAAATCACATTTTTTGCCTTTGGCTTGAGTGTAGACCTTAAGCGAGGAGCAGGATCTTCTTTCAGACTTATGGAATAATGCGCGGTTTGTCATATCTCtttt                                                                                                             <  1:173409/140‑1 (MQ=255)                       taAGTTCATTCCAGCTCACAGTGAAATCAGATGTGTACGAAATCACATTTTTTGCCTTTGGCTTGAGTGTAGACCTTAAGCGAGGAGCAGGATCTTCTTTCAGACTTATGGAATAATGCGCGGTTTGTCATATCTCtttt                                                                                                             <  1:418204/140‑1 (MQ=255)                               ttCCAGCTCACAGTGAAATCAGATGTGTACGAAATCACATTTTTTGCCTTTGGCTTGAGTGTAGACCTTAAGCGAGGAGCAGGATCTTCTTTCAGACTTATGGAATAATGCGCGGTTTGTCATAtct                                                                                                                  <  1:70750/127‑1 (MQ=255)                               ttCCAGCTCACAGTGAAATCAGATGTGTACGAAATCACATTTTTTGCCTTTGGCTTGAGTGTAGACCTTAAGCGAGGAGCAGGATCTTCTTTCAGACTTATGGAATAATGCGCGGTTTGTCATAtct                                                                                                                  >  2:70750/1‑127 (MQ=255)                               ttCCAGCTCACAGTGAAATCAGATGTGTACGAAATCACATTTTTTGCCTTTGGCTTGAGTGTAGACCTTAAGCGAGGAGCAGGATCTTCTTTCAGACTTATGGAATAATGCGCGGTTTGTCATATCTCTTTTCAGGATa                                                                                                      <  1:263250/139‑1 (MQ=255)                               ttCCAGCTCACAGTGAAATCAGATGTGTACGAAATCACATTTTTTGCCTTTGGCTTGAGTGTAGACCTTAAGCGAGGAGCAGGATCTTCTTTCAGACTTATGGAATAATGCGCGGTTTGTCATATCTCTTTTCAGGATa                                                                                                      >  2:263250/1‑139 (MQ=255)                               ttCCAGCTCACAGTGAAATCAGATGTGTACGAAATCACATTTTTTGCCTTTGGCTTGAGTGTAGACCTTAAGCGAGGAGCAGGATCTTCTTTCAGACTTATGGAATAATGCGCGGTTTGTCATATCTCTTTTCAGGATAc                                                                                                     >  2:692441/1‑140 (MQ=255)                               ttCCAGCTCACAGTGAAATCAGATGTGTACGAAATCACATTTTTTGCCTTTGGCTTGAGTGTAGACCTTAAGCGAGGAGCAGGATCTTCTTTCAGACTTATGGAATAATGCGCGGTTTGTCATATCTCTTTTCAGGATAc                                                                                                     >  1:3415/1‑140 (MQ=255)                                     cTCACAGTGAAATCAGATGTGTACGAAATCACATTTTTTGCCTTTGGCTTGAGTGTAGACCTTAAGCGAGGAGCAGGATCTTCTTTCAGACTTATGGAATAATGCGCGGTTTGTCATATCTCTTTTCAGGATACGc                                                                                                   <  2:887918/136‑1 (MQ=255)                                     cTCACAGTGAAATCAGATGTGTACGAAATCACATTTTTTGCCTTTGGCTTGAGTGTAGACCTTAAGCGAGGAGCAGGATCTTCTTTCAGACTTATGGAATAATGCGCGGTTTGTCATATCTCTTTTCAGGATACGc                                                                                                   >  1:887918/1‑136 (MQ=255)                                          aGTGAAATCAGATGTGTACGAAATCACATTTTTTGCCTTTGGCTTGAGTGTAGACCTTAAGCGAGGAGCAGGATCTTCTTTCAGACTTATGGAATAATGCGCGGTTTGTCATATCTCTTTTCAGGATACGCCTGTGTTAg                                                                                          >  1:882370/1‑140 (MQ=255)                                               aaTCAGATGTGTACGAAATCACATTTTTTGCCTTTGGCTTGAGTGTAGACCTTAAGCGAGGAGCAGGATCTTCTTTCAGACTTATGGAATAATGCGCGGTTTGTCATATCTCTTTTCAGGATACGCCTGTGTTAGTTTcc                                                                                     <  2:374014/140‑1 (MQ=255)                                                  cAGATGTGTACGAAATCACATTTTTTGCCTTTGGCTTGAGTGTAGACCTTAAGCGAGGAGCAGGATCTTCTTTCAGACTTATGGAATAATGCGCGGTTTGTCATATCTCTTTTCAGGATACGCCTGTGTTAGTTTCCAGt                                                                                  <  2:882370/140‑1 (MQ=255)                                                    gATGTGTACGAAATCACATTTTTTGCCTTTGGCTTGAGTGTAGACCTTAAGCGAGGAGCAGGATCTTCTTTCAGACTTATGGAATAATGCGCGGTTTGTCATATCTCTTTTCAGGATACGCCTGTGTTAGTTTCCAGTaa                                                                                <  2:167644/140‑1 (MQ=255)                                                      tgtgTACGAAATCACATTTTTTGCCTTTGGCTTGAGTGTAGACCTTAAGCGAGGAGCAGGATCTTCTTTCAGACTTATGGAATAATGCGCGGTTTGTCATATCTCTTTTCAGGATACGCCTGTGTTAGTTTCCAGTAACg                                                                              <  1:575532/140‑1 (MQ=255)                                                         gtACGAAATCACATTTTTTGCCTTTGGCTTGAGTGTAGACCTTAAGCGAGGAGCAGGATCTTCTTTCAGACTTATGGAATAATGCGCGGTTTGTCATATCTCTTTTCAGGATACGCCTGTGTTAGTTTCCAGTAACGTCa                                                                           <  2:238473/140‑1 (MQ=255)                                                            cGAAATCACATTTTTTGCCTTTGGCTTGAGTGTAGACCTTAAGCGAGGAGCAGGATCTTCTTTCAGACTTATGGAATAATGCGCGGTTTGTCATATCGCTTTTCAGGATACGCCTGTGTTAGTTTCCAGTAACGTCACCa                                                                        <  2:782029/140‑1 (MQ=255)                                                                   acaTTTTTTGCCTTTGGCTTGAGTGTAGACCTTAAGCGAGGAGCAGGATCTTCTTTCAGACTTATGGAATAATGCGCGGTTTGTCATATCTCTTTTCAGGATACGCCTGTGTTAGTTTCCAGTAACGTCACCATGCAGtt                                                                 <  1:786648/140‑1 (MQ=255)                                                                    caTTTTTTGCCTTTGGCTTGAGTGTAGACCTTAAGCGAGGAGCAGGATCTTCTTTCAGACTTATGGAATAATGCGCGGTTTGTCATATCTCTTTTCAGGATACGCCTGTGTTAGTTTCCAGTAACGTCACCATGCAGTTc                                                                >  1:303455/1‑140 (MQ=255)                                                                           tGCCTTTGGCTTGAGTGTAGACCTTAAGCGAGGAGCAGGATCTTCTTTCAGACTTATGGAATAATGCGCGGTTTGTCATATCTCTTTTCAGGATACGCCTGTGTTAGTTTCCAGTAACGTCACCATGCAGTTCGGCAGTa                                                         >  2:755584/1‑140 (MQ=255)                                                                                ttGGCTTGAGTGTAGACCTTAAGCGAGGAGCAGGATCTTCTTTCAGACTTATGGAATAATGCGCGGTTTGTCATATCTCTTTTCAGGATACGCCTGTGTTAGTTTCCAGTAACGTCACCATGCAGTTCGGCAGTAAGCCg                                                    <  1:692441/140‑1 (MQ=255)                                                                                 tGGCTTGAGTGTAGACCTTAAGCGAGGAGCGGGATCTTCTTTCAGACTTATGGAATAATGCGCGGTTTGTCATATCTCTTTTCAGGATACGCCTGTGTTAGTTTCCAGTAACGTCACCATGCAGTTCGGCAGTAAGCCgt                                                   >  1:26773/1‑140 (MQ=255)                                                                                                  ttAAGCGAGGAGCAGGATCTTCTTTCAGACTTATGGAATAATGCGCGGTTTGTCATATCTCTTTTCAGGATACGCCTGTGTTAGTTTCCAGTAACGTCACCATGCAGTTCGGCAGTAAGCCGTTGttt                                              >  2:786611/1‑128 (MQ=255)                                                                                                  ttAAGCGAGGAGCAGGATCTTCTTTCAGACTTATGGAATAATGCGCGGTTTGTCATATCTCTTTTCAGGATACGCCTGTGTTAGTTTCCAGTAACGTCACCATGCAGTTCGGCAGTAAGCCGTTGttt                                              <  1:786611/128‑1 (MQ=255)                                                                                                  ttAAGCGAGGAGCAGGATCTTCTTTCAGACTTATGGAATAATGCGCGGTTTGTCATATCTCTTTTCAGGATACGCCTGTGTTAGTTTCCAGTAACGTCACCATGCAGTTCGGCAGTAAGCCGTTGTTTGAAAACATTTcc                                  >  2:192819/1‑140 (MQ=255)                                                                                                  ttAAGCGAGGAGCAGGATCTTCTTTCAGACTTATGGAATAATGCGCGGTTTGTCATATCTCTTTTCAGGATACGCCTGTGTTAGTTTCCAGTAACGTCACCATGCAGTTCGGCAGTAAGCCGTTGTTTGAAAACATTTcc                                  >  2:414854/1‑140 (MQ=255)                                                                                                         aggagCAGGATCTTCTTTCAGACTTATGGAATAATGCGCGGTTTGTCATATCTCTTTTCAGGATACGCCTGTGTTAGTTTCCAGTAACGTCACCATGCAGTTCGGCAGTAAGCCGTTGTTTGAAAACATTTCCGTCAAAt                           >  1:509627/1‑140 (MQ=255)                                                                                                         aggagCAGGATCTTCTTTCAGACTTATGGAATAATGCGCGGTTTGTCATATCTCTTTTCAGGATACGCCTGTGTTAGTTTCCAGTAACGTCACCATGCAGTTCGGCAGTAAGCCGTTGTTTGAAAACATTTCCGTCAAAt                           >  1:806950/1‑140 (MQ=255)                                                                                                         aggagCAGGATCTTCTTTCAGACTTATGGAATAATGCGCGGTTTGTCATATCTCTTTTCAGGATACGCCTGTGTTAGTTTCCAGTAACGTCACCATGCAGTTCGGCAGTAAGCCGTTGTTT‑AAAACATTTCCGTCAAAtt                          >  2:888496/1‑140 (MQ=255)                                                                                                           gagCAGGATCTTCTTTCAGACTTATGGAATAATGCGCGGTTTGTCATATCTCTTTTCAGGATACGCCTGTGTTAGTTTCCAGTAACGTCACCATGCAGTTCGGCAGTAAGCCGTTGTTTGAAAACATTTCCGTCAAAttt                         >  1:517541/1‑140 (MQ=255)                                                                                                                      tcttTCAGACTTATGGAATAATGCGCGGTTTGTCATATCTCTTTTCAGGATACGCCTGTGTTAGTTTCCAGTAACGTCACCATGCAGTTCGGCAGTAAGCCGTTGTTTGAAAACATTTCCGTCAAATTTggcggcg                  <  1:181720/136‑1 (MQ=255)                                                                                                                      tcttTCAGACTTATGGAATAATGCGCGGTTTGTCATATCTCTTTTCAGGATACGCCTGTGTTAGTTTCCAGTAACGTCACCATGCAGTTCGGCAGTAAGCCGTTGTTTGAAAACATTTCCGTCAAATTTggcggcg                  >  2:181720/1‑136 (MQ=255)                                                                                                                        tttCAGACTTATGGAATAATGCGCGGTTTGTCATATCTCTTTTCAGGATACGCCTGTGTTAGTTTCCAGTAACGTCACCATGCAGTTCGGCAGTAAGCCGTTGTTTGAAAACATTTCCGTCAAATTTGGCGGCGGCAAcc            >  1:443123/1‑140 (MQ=255)                                                                                                                           cAGACTTATGGAATAATGCGCGGTTTGTCATATCTCTTTTCAGGATACGCCTGTGTTAGTTTCCAGTAACGTCACCATGCAGTTCGGCAGTAAGCCGTTGTTTGAAAACATTTCCGTCAAATTTGGCGGCGGCaa              <  1:93403/135‑1 (MQ=255)                                                                                                                           cAGACTTATGGAATAATGCGCGGTTTGTCATATCTCTTTTCAGGATACGCCTGTGTTAGTTTCCAGTAACGTCACCATGCAGTTCGGCAGTAAGCCGTTGTTTGAAAACATTTCCGTCAAATTTGGCGGCGGCaa              >  2:93403/1‑135 (MQ=255)                                                                                                                           cAGACTTATGGAATAATGCGCGGTTTGTCATATCTCTTTTCAGGATACGCCTGTGTTAGTTTCCAGTAACGTCACCATGCAGTTCGGCAGTAAGCCGTTGTTTGAAAACATTTCCGTCAAATTTGGCGGCGGCAACCGtt         >  2:759215/1‑140 (MQ=255)                                                                                                                             gACTTATGGAATAATGCGCGGTTTGTCATATCTCTTTTCAGGATACGCCTGTGTTAGTTTCCAGTAACGTCACCATGCAGTTCGGCAGTAAGCCGTTGTTTGAAAACATTTCCGTCAAATTTGGCGGCGGCAACCGTTAc       >  2:758897/1‑140 (MQ=255)                                                                                                                                  aTGGAATAATGCGCGGTTTGTCATATCTCTTTTCAGGATACGCCTGTGTTAGTTTCCAGTAACGTCACCATGCAGTTCGGCAGTAAGCCGTTGTTTGAAAACATTTCCGTCAAATTTGGCGGCGGCAACCGTTACGGCCt  >  2:884533/1‑140 (MQ=255)                                                                                                                                      |                                                                                                                                        TTAGAGTATTGGAAGCGCATTATAAGTTCATTCCAGCTCACAGTGAAATCAGATGTGTACGAAATCACATTTTTTGCCTTTGGCTTGAGTGTAGACCTTAAGCGAGGAGCAGGATCTTCTTTCAGACTTATGGCATAATGCGCGGTTTGTCATATCTCTTTTCAGGATACGCCTGTGTTAGTTTCCAGTAACGTCACCATGCAGTTCGGCAGTAAGCCGTTGTTTGAAAACATTTCCGTCAAATTTGGCGGCGGCAACCGTTACGGCCT  >  NC_000913/855789‑856057

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑