Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	922,738	G→T	intergenic (‑148/‑175)	cspD ← / → clpS	DNA replication inhibitor/specificity factor for ClpA‑ClpP chaperone‑protease complex

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	922,738	0	G	T	100.0%	198.7 / NA	57	intergenic (‑148/‑175)	cspD/clpS	DNA replication inhibitor/specificity factor for ClpA‑ClpP chaperone‑protease complex
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (27/30);  total (27/30)

CGCAAATCTTATACAAGTAAGATGGAATAAACCGGGGTCAGAGAGGGGGCTGTTCAAAACCTCGCCAACTCTAGAAATACAATTTAGAGAATTAGGGCGAGCCGTCAAGCATTTGACAGGGGACAAGGGGCAGGTATGAATCAAAAATTTGAAGCAGTTAACGCTATTGACAGGAATGTGACAGATGTCGCTGATGCCAACGATAGATGATAGTTATCTATCATGTGGAGTAGATTGGTCAGGCAAATAAGCTCTTGTCAG  >  NC_000913/922605‑922865                                                                                                                                      |                                                                                                                                cGCAAATCTTATACAAGTAAGATGGAATAAACCGGGGTCAGAGAGGGGGCTGTTCAAAACCTCGCCAACTCTAGAAATACAATTTAGAGAATTAGGGCGAGCCGTCAAGCATTTGACAGGGGACAAGGGGCAGTTATGaa                                                                                                                           >  2:107180/1‑140 (MQ=255)              cAAGTAAGATGGAATAAACCGGGGTCAGAGAGGGGGCTGTTCAAAACCTCGCCAACTCTAGAAATACAATTTAGAGAATTAGGGCGAGCCGTCAAGCATTTGACAGGGGACAAGGGGCAGTTATGAATCAAAAATTTGaa                                                                                                              <  2:841075/140‑1 (MQ=255)               aaGTAAGATGGAATAAACCGGGGTCAGAGAGGGGGCTGTTCAAAACCTCGCCAACTCTAGAAATACAATTTAGAGAATTAGGGCGAGCCGTCAAGCATTTGACAGGGGACAAGGGGCAGTTATGAATCAAAAATTTGAAg                                                                                                             <  1:494465/140‑1 (MQ=255)                aGTAAGATGGAATAAACCGGGGTCAGAGAGGGGGCTGTTCAAAACCTCGCCAACTCTAGAAATACAATTTAGAGAATTAGGGCGAGCCGTCAAGCATTTGACAGGGGACAAGGGGCAGTTATGAATCAAAAATTTGAAGc                                                                                                            <  1:262138/140‑1 (MQ=255)                    aGATGGAATAAACCGGGGTCAGAGAGGGGGCTGTTCAAAACCTCGCCAACTCTAGAAATACAATTTAGAGAATTAGGGCGAGCCGTCAAGCATTTGACAGGGGACAAGGGGCAGTTATGAATCAAAAATTTGAAGCAGtt                                                                                                        >  1:715710/1‑140 (MQ=255)                      aTGGAATAAACCGGGGTCAGAGAGGGGGCTGTTCAAAACCTCGCCAACTCTAGAAATACAATTTAGAGAATTAGGGCGAGCCGTCAAGCATTTGACAGGGGACAAGGGGCAGTTATGAATCAAAAATTTGAAGCa                                                                                                           <  1:145978/135‑1 (MQ=255)                      aTGGAATAAACCGGGGTCAGAGAGGGGGCTGTTCAAAACCTCGCCAACTCTAGAAATACAATTTAGAGAATTAGGGCGAGCCGTCAAGCATTTGACAGGGGACAAGGGGCAGTTATGAATCAAAAATTTGAAGCa                                                                                                           >  2:145978/1‑135 (MQ=255)                      aTGGAATAAACCGGGGTCAGAGAGGGGGCTGTTCAAAACCTCGCCAACTCTAGAAATACAATTTAGAGAATTAGGGCGAGCCGTCAAGCATTTGACAGGGGACAAGGGGCAGTTATGAATCAAAAATTTGAAGCAGTTaa                                                                                                      >  1:126931/1‑140 (MQ=255)                      aTGGAATAAACCGGGGTCAGAGAGGGGGCTGTTCAAAACCTCGCCAACTCTAGAAATACAATTTAGAGAATTAGGGCGAGCCGTCAAGCATTTGACAGGGGACAAGGGGCAGTTATGAATCAAAAATTTGAAGCAGTTaa                                                                                                      <  2:436570/140‑1 (MQ=255)                               aCCGGGGTCAGAGAGGGGGCTGTTCAAAAACTCGCCAACTCTAGAAATACAATTTAGAGAATTAGGGCGAGCCGTCAAGCATTTGACAGGGGACAAGGGGCAGTTATGAATCAAAAATTTGAAGCAGTTAACGCTATTGa                                                                                             >  1:60332/1‑140 (MQ=255)                                ccGGGGTCAGAGAGGGGGCTGTTCAAAACCTCGCCAACTCTAGAAATACAATTTAGAGAATTAGGGCGAGCCGTCAAGCATTTGACAGGGGACAAGGGGCAGTTATGAATCAAAAATTTGAAGCAGTTAACGCTATTGAc                                                                                            <  1:222762/140‑1 (MQ=255)                                    ggTCAGAGAGGGGGCTGTTCAAAACCTCGCCAACTCTAGAAATACAATTTAGAGAATTAGGGCGAGCCGTCAAGCATTTGACAGGGGACAAGGGGCAGTTATGAATCAAAAATTTGAAGCAGTTAACGCTATTGACAGGa                                                                                        >  2:103199/1‑140 (MQ=255)                                    ggTCAGAGAGGGGGCTGTTCAAAACCTCGCCAACTCTAGAAATACAATTTAGAGAATTAGGGCGAGCCGTCAAGCATTTGACAGGGGACAAGGGGCAGTTATGAATCAAAAATTTGAAGCAGTTAACGCTATTGACAGGa                                                                                        >  2:309583/1‑140 (MQ=255)                                              ggggCTGTTCAAAACCTCGCCAACTCTAGAAATACAATTTAGAGAATTAGGGCGAGCCGTCAAGCATTTGACAGGGGACAAGGGGCAGTTATGAATCAAAAATTTGAAGCAGTTAACGCTATTGACAGGAATGTGACAGa                                                                              >  1:313440/1‑140 (MQ=255)                                                ggCTGTTCAAAACCTCGCCAACTCTAGAAATACAATTTAGAGAATTAGGGCGAGCCGTCAAGCATTTGACAGGGGACAAGGGGCAGTTATGAATCAAAAATTTGAAGCAGTTAACGCTATTGACAGGAATGTGACAGATg                                                                            >  1:284678/1‑140 (MQ=255)                                                  cTGTTCAAAACCTCGCCAGCTCTAGAAATACGATTTAGAGAATTAGGGCGAGCCGTCAAGCATTTGACAGGGGACAAGGGGCAGTTATGAATCAAAAATTTGAAGCAGTTAACGCTATTGACGGGAATGTGACAGATGTc                                                                          >  1:697944/1‑140 (MQ=255)                                                  cTGTTCAAAACCTCGCCAACTCTAGAAATACAATTTAGAGAATTAGGGCGAGCCGTCAAGCATTTGACAGGGGACAAGGGGCAGTTATGAATCAAAAATTTGAAGCAGTTAACGCTATTGACAGGAATGTGACAGATGTc                                                                          >  1:541470/1‑140 (MQ=255)                                                     ttCAAAACCTCGCCAACTCTAGAAATACAATTTAGAGAATTAGGGCGAGCCGTCAAGCATTTGACAGGGGACAAGGGGCAGTTATGAATCAAAAATTTGAAGCAGTTAACGCTATTGACAGGAATGTGACAGATGTCGCt                                                                       >  2:67359/1‑140 (MQ=255)                                                      tCAAAACCTCGCCAACTCTAGAAATACAATTTAGAGAATTAGGGCGAGCCGTCAAGCATTTGACAGGGGACAAGGGGCAGTTATGAATCAAAAATTTGAAGCAGTTAACGCTATTGACAGGAATGTGACAGATGTCGCTg                                                                      <  2:605037/140‑1 (MQ=255)                                                        aaaaCCTCGCCAACTCTAGAAATACAATTTAGAGAATTAGGGCGAGCCGTCAAGCATTTGACAGGGGACAAGGGGCAGTTATGAATCAAAAATTTGAAGCAGTTAACGCTATTGACAGGAATGTGACAGATGTCGCTGAt                                                                    <  1:107180/140‑1 (MQ=255)                                                          acccTCGCCAACTCTAGAAATACAATTTAGAGAATTAGGGCGAGCCGTCAAGCATTTGACAGGGGACAAGGGGCAGTTATGAATCAAAAATTTGAAGCAGTTAACGCTATTGACAGGAATGTGACAGATGTCGCTGATGc                                                                  <  2:310124/138‑1 (MQ=255)                                                          aaCCTCGCCAACTCTAGAAATACAATTTAGAGAATTAGGGCGAGCCGTCAAGCATTTGACAGGGGACAAGGGGCAGTTATGAATCAAAAATTTGAAGCAGTTAACGCTATTGACAGGAATGTGACAGATGTCGCTGATGc                                                                  >  1:19972/1‑140 (MQ=255)                                                           aCCTCGCCAACTCTAGAAATACAATTTAGAGAATTAGGGCGAGCCGTCAAGCATTTGACAGGGGACAAGGGGCAGTTATGAATCAAAAATTTGAAGCAGTTAACGCTATTGACAGGAATGTGACAGATGTCGCTGATGcc                                                                 <  1:628010/140‑1 (MQ=255)                                                             cTCGCCAACTCTAGAAATACAATTTAGAGAATTAGGGCGAGCCGTCAAGCATTTGACAGGGGACAAGGGGCAGTTATGAATCAAAAATTTGAAGCAGTTAACGCTATTGACAGGAATGTGACAGATGTCGCTGATGCCaa                                                               <  2:284678/140‑1 (MQ=255)                                                               cGCCAACTCTAGAAATACAATTTAGAGAATTAGGGCGAGCCGTCAAGCATTTGACAGGGGACAAGGGGCAGTTATGAATCAAAAATTTGAAGCAGTTAACGCTATTGACAGGAATGTGACAGATGTCGCTg                                                                      >  1:67791/1‑131 (MQ=255)                                                               cGCCAACTCTAGAAATACAATTTAGAGAATTAGGGCGAGCCGTCAAGCATTTGACAGGGGACAAGGGGCAGTTATGAATCAAAAATTTGAAGCAGTTAACGCTATTGACAGGAATGTGACAGATGTCGCTg                                                                      <  2:67791/131‑1 (MQ=255)                                                               cGCCAACTCTAGAAATACAATTTAGAGAATTAGGGCGAGCCGTCAAGCATTTGACAGGGGACAAGGGGCAGTTATGAATCAAAAATTTGAAGCAGTTAACGCTATTGACAGGAATGTGACAGATGTCGCTGATGCCAACg                                                             <  1:818325/140‑1 (MQ=255)                                                               cGCCAACTCTAGAAATACAATTTAGAGAATTAGGGCGAGCCGTCAAGCATTTGACAGGGGACAAGGGGCAGTTATGAATCAAAAATTTGAAGCAGTTAACGCTATTGACAGGAATGTGACAGATGTCGCTGATGCCAACg                                                             >  1:463544/1‑140 (MQ=255)                                                               cGCCAACTCTAGAAATACAATTTAGAGAATTAGGGCGAGCCGTCAAGCATTTGACAGGGGACAAGGGGCAGTTATGAATCAAAAATTTGAAGCAGTTAACGCTATTGACAGGAATGTGACAGATGTCGCTGATGCCAACg                                                             >  2:301519/1‑140 (MQ=255)                                                                gCCAGCTCTAGAAATACAATTTAGAGAATTAGGGCGAGCCGTCAAGCATTTGACAGGGGACAAGGGGCAGTTATGAATCAAAAATTTGAAGCAGTTAACGCTATTGACGGGAATGTGACAGATGTCGCTGATGCCAACGa                                                            <  2:697944/140‑1 (MQ=255)                                                                gCCAACTCTAGAAATACAATTTAGAGAATTAGGGCGAGCCGTCAAGCATTTGACAGGGGACAAGGGGCAGTTATGAATCAAAAATTTGAAGCAGTTAACGCTATTGACAGGAATGTGACAGATGTCGCTGATGCCAACGa                                                            <  1:301519/140‑1 (MQ=255)                                                                   aaCTCTAGAAATACAATTTAGAGAATTAGGGCGAGCCGTCAAGCATTTGACAGGGGACAAGGGGCAGTTATGAATCAAAAATTTGAAGCAGTTAACGCTATTGACAGGAATGTGACAGATGTCGCTGATGCCAACGATAg                                                         <  2:126931/140‑1 (MQ=255)                                                                        tAGAAATACAATTTAGAGAATTAGGGCGAGCCGTCAAGCATTTGACAGGGGACAAGGGGCAGTTATGAATCAAAAATTTGAAGCAGTTAACGCTATTGACAGGAATGTGACAGATGTCGCTGATGCCAACGATAgatgat                                                    >  1:413609/1‑140 (MQ=255)                                                                         aGAAATACAATTTAGAGAATTAGGGCGAGCCGTCAAGCATTTGACAGGGGACAAGGGGCAGTTATGAATCAAAAATTTGAAGCAGTTAACGCTATTGACAGGAATGTGACAGATGTCGCTGATGCCAACGATAGATGATa                                                   <  2:32331/140‑1 (MQ=255)                                                                            aaTACAATTTAGAGAATTAGGGCGAGCCGTCAAGCATTTGACAGGGGACAAGGGGCAGTTATGAATCAAAAATTTGAAGCAGTTAACGCTATTGACAGGAATGTGACAGATGTCGCTGATGCCAACGATAGATGATAGtt                                                <  2:503763/140‑1 (MQ=255)                                                                                cAATTTAGAGAATTAGGGCGAGCCGTCAAGCATTTGACAGGGGACAAGGGGCAGTTATGAATCAAAAATTTGAAGCAGTTAACGCTATTGACAGGAATGTGACAGATGTCGCTGATGCCAACGATAGATGATAGTtatct                                            >  2:877477/1‑140 (MQ=255)                                                                                cAATTTAGAGAATTAGGGCGAGCCGTCAAGCATTTGACAGGGGACAAGGGGCAGTTATGAATCAAAAATTTGAAGCAGTTAACGCTATTGACAGGAATGTGACAGATGTCGCTGATGCCAACGATAGATGATAGTtatct                                            <  1:309583/140‑1 (MQ=255)                                                                                     tAGAGAATTAGGGCGAGCCGTCAAGCATTTGACAGGGGACAAGGGGCAGTTATGAATCAAAAATTTGAAGCAGTTAACGCTATTGACAGGAATGTGACAGATGTTGCTGATGCCAACGATAGATGATAGTTATCTATCAt                                       >  1:678321/1‑140 (MQ=255)                                                                                     tAGAGAATTAGGGCGAGCCGTCAAGCATTTGACAGGGGACAAGGGGCAGTTATGAATCAAAAATTTGAAGCAGTTAACGCTATTGACAGGAATGTGACAGATGTCGCTGATGCCAACGATAGATGATAGTTATCTATCAt                                       <  2:463544/140‑1 (MQ=255)                                                                                        agaATTAGGGCGAGCCGTCAAGCATTTGACAGGGGACAAGGGGCAGTTATGAATCAAAAATTTGAAGCAGTTAACGCTATTGACAGGAATGTGACAGATGTCGCTGATGCCAACGATAGATGATAGTTATCTATCAtgtg                                    <  2:678321/140‑1 (MQ=255)                                                                                        agaATTAGGGCGAGCCGTCAAGCATTTGACAGGGGACAAGGGGCAGTTATGAATCAAAAATTTGAAGCAGTTAACGCTATTGACAGGAATGTGACAGATGTCGCTGATGCCAACGATAGATGATAGTTATCTATCAt                                       >  1:498859/1‑137 (MQ=255)                                                                                        agaATTAGGGCGAGCCGTCAAGCATTTGACAGGGGACAAGGGGCAGTTATGAATCAAAAATTTGAAGCAGTTAACGCTATTGACAGGAATGTGACAGATGTCGCTGATGCCAACGATAGATGATAGTTATCTATCAt                                       <  2:498859/137‑1 (MQ=255)                                                                                             tAGGGCGAGCCGTCAAGCATTTGACAGGGGACAAGGGGCAGTTATGAATCAAAAATTTGAAGCAGTTAACGCTATTGACAGGAATGTGACAGATGTCGCTGATGCCAACGATAGATGATAGTTATCTATCATGTGGa                                  >  2:325191/1‑137 (MQ=255)                                                                                             tAGGGCGAGCCGTCAAGCATTTGACAGGGGACAAGGGGCAGTTATGAATCAAAAATTTGAAGCAGTTAACGCTATTGACAGGAATGTGACAGATGTCGCTGATGCCAACGATAGATGATAGTTATCTATCATGTGGa                                  <  1:325191/137‑1 (MQ=255)                                                                                               gggCGAGCCGTCAAGCATTTGACAGGGGACAAGGGGCAGTTATGAATCAAAAATTTGAAGCAGTTAACGCTATTGACAGGAATGTGACAGATGTCGCTGATGCCAACGATAGATGATAGTtat                                              >  2:131773/1‑123 (MQ=255)                                                                                               gggCGAGCCGTCAAGCATTTGACAGGGGACAAGGGGCAGTTATGAATCAAAAATTTGAAGCAGTTAACGCTATTGACAGGAATGTGACAGATGTCGCTGATGCCAACGATAGATGATAGTtat                                              <  1:131773/123‑1 (MQ=255)                                                                                               gggCGAGCCGTCAAGCATTTGACAGGGGACAAGGGGCAGTTATGAATCAAAAATTTGAAGCAGTTAACGCTATTGACAGGAATGTGACAGATGTCGCTGATGCCAACGATAGATGATAGTTATCTATCATGTGGAGTAGa                             <  2:652745/140‑1 (MQ=255)                                                                                                    aGCCGTCAAGCATTTGACAGGGGACAAGGGGCAGTTATGAATCAAAAATTTGAAGCAGTTAACGCTATTGACAGGAATGTGACAGATGTCGCTGATGCCAACGATAGATGATAGTTATCTATCATGTGGAGTAGATTGGt                        >  2:12110/1‑140 (MQ=255)                                                                                                          cAAGCATTTGACAGGGGACAAGGGGCAGTTATGAATCAAAAATTTGAAGCAGTTAACGCTATTGACAGGAATGTGACAGATGTCGCTGATGCCAACGATAGATGATAGTTATCTATCATGTGGAGTAGa                             <  1:47015/129‑1 (MQ=255)                                                                                                          cAAGCATTTGACAGGGGACAAGGGGCAGTTATGAATCAAAAATTTGAAGCAGTTAACGCTATTGACAGGAATGTGACAGATGTCGCTGATGCCAACGATAGATGATAGTTATCTATCATGTGGAGTAGa                             >  2:47015/1‑129 (MQ=255)                                                                                                           aaGCATTTGACAGGGGACAAGGGGCAGTTATGAATCAAAAATTTGAAGCAGTTAACGCTATTGACAGGAATGTGACAGATGTCGCTGATGCCAACGATAGATGATAGTTATCTATCATGTGGAGTAGATTGGTCAGGCaa                 <  1:877477/140‑1 (MQ=255)                                                                                                                  tGACAGGGGACAAGGGGCAGTTATGAATCAAAAATTTGAAGCAGTTAACGCTATTGACAGGAATGTGACAGATGTCGCTGATGCCAACGATAGATGATAGTTATCTATCATGTGGAGTAGATTGGTCAGGCAAATAAGct          >  1:520307/1‑140 (MQ=255)                                                                                                                   gACAGGGGACAAGGGGCAGTTATGAATCAAAAATTTGAAGCAGTTAACGCTATTGACAGGAATGTGACAGATGTCGCTGATGCCAACGATAGATGATAGTTATCTATCATGTGGAGTAGATTGGTCAGGCAAATAAGctc         <  2:541470/140‑1 (MQ=255)                                                                                                                   gACAGGGGACAAGGGGCAGTTATGAATCAAAAATTTGAAGCAGTTAACGCTATTGACAGGAATGTGACAGATGTCGCTGATGCCAACGATAGATGATAGTTATCTATCATGTGGAGTAGATTGGTCAGGCAAATAAGctc         <  2:715710/140‑1 (MQ=255)                                                                                                                   gACAGGGGACAAGGGGCAGTTATGAATCAAAAATTTGAAGCAGTTAACGCTATTGACAGGAATGTGACAGATGTCGCTGATGCCAACGATAGATGATAGTTATCTATCATGTGGAGTAGATTGGTCAGGCAAATAAGctc         >  2:848726/1‑140 (MQ=255)                                                                                                                       ggggACAAGGGGCAGTTATGAATCAAAAATTTGAAGCAGTTAACGCTATTGACAGGAATGTGACAGATGTCGCTGATGCCAACGATAGATGATAGTTATCTATCATGTGGAGTAGATTGGTCAGGCAAATAAGCTCTTGt     <  1:848726/140‑1 (MQ=255)                                                                                                                          gACAAGGGGCAGTTATGAATCAAAAATTTGAAGCAGTTAACGCTATTGACAGGAATGTGACAGATGTCGCTGATGCCAACGATAGATGATAGTTATCTATCATGTGGAGTAGATTGGTCAGGCAAATAAGCTCTTGTCAg  >  2:257982/1‑140 (MQ=255)                                                                                                                                      |                                                                                                                                CGCAAATCTTATACAAGTAAGATGGAATAAACCGGGGTCAGAGAGGGGGCTGTTCAAAACCTCGCCAACTCTAGAAATACAATTTAGAGAATTAGGGCGAGCCGTCAAGCATTTGACAGGGGACAAGGGGCAGGTATGAATCAAAAATTTGAAGCAGTTAACGCTATTGACAGGAATGTGACAGATGTCGCTGATGCCAACGATAGATGATAGTTATCTATCATGTGGAGTAGATTGGTCAGGCAAATAAGCTCTTGTCAG  >  NC_000913/922605‑922865

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑