Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,234,451	C→A	V89V (GTG→GTT)	nhaB ←	Na(+):H(+) antiporter NhaB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,234,451	0	C	A	100.0%	120.4 / NA	36	V89V (GTG→GTT)	nhaB	Na(+):H(+) antiporter NhaB
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (17/19);  total (17/19)

AGCGACAGCAGCATTTTGGAGCGAATGCTTAACAGCAAACGGGTAAATATGAACAGCAACAGCTGTTTCATAAAATAGATACCCGCCACCATAAACATCAGTAACAGCAAGACTTCAAGATTTGCCGCCACCTCTTCACGGACGTGTTCCGCGCTGGTCATGCCGATGAATACCGCTTCGATAGCCAACAGACCACCGGGGAGCAGCGGGTAGCATTTCAGGGCCATCGCCAGAGTGAAAATAAATTCCGCTACC  >  NC_000913/1234323‑1234577                                                                                                                                 |                                                                                                                               agCGACAGCAGCATTTTGGAGCGAATGCTTAACAGCAAACGGGTAAATATGAACAGCAACAGCTGTTTCATAAAATAGATACCCGCCACCATAAACATCAGTAACAGCAAGACTTCAAGATTTGCCGCAACCTCTTCACg                                                                                                                     <  2:370151/140‑1 (MQ=255)   cGACAGCAGCATTTTGGAGCGAATGCTTAACAGCAAACGGGTAAATATGAACAGTAACAGCTGTTTCATAAAATAGATACCCGCCACCATAAACATCAGTAACAGCAAGACTTCAAGATTTGCCGCAACCTCTTCACGGa                                                                                                                   >  1:554028/1‑140 (MQ=255)   cGACAGCAGCATTTTGGAGCGAATGCTTAACAGCAAACGGGTAAATATGAACAGCAACAGCTGTTTCATAAAATAGATACCCGCCACCATAAACATCAGTAACAGCAAGACTTCAAGATTTGCCGCAACCTCTTCACGGa                                                                                                                   >  1:206185/1‑140 (MQ=255)    gACAGCAGCATTTTGGAGCGAATGCTTAACAGCAAACGGGTAAATATGAACAGCAACAGCTGTTTCATAAAATAGATACCCGCCACCATAAACATCAGTAACAGCAAGACTTCAAGATTTGCCGCAACCTCTTCACGGAc                                                                                                                  <  1:594813/140‑1 (MQ=255)                       gAATGCTTAACAGCAAACGGGTAAATATGAACAGCAACAGCTGTTTCATAAAATAGATACCCGCCACCATAAACATCAGTAACAGCAAGACTTCAAGATTTGCCGCAACCTCTTCACGGACGTGTTCCGCGCTGGTCATg                                                                                               <  2:281833/140‑1 (MQ=255)                       gAATGCTTAACAGCAAACGGGTAAATATGAACAGCAACAGCTGTTTCATAAAATAGATACCCGCCACCATAAACATCAGTAACAGCAAGACTTCAAGATTTGCCGCAACCTCTTCACGGACGTGTTCCGCGCTGGTCATg                                                                                               <  2:846756/140‑1 (MQ=255)                        aaTGCTTAACAGCAAACGGGTAAATATGAACAGCAACAGCTGTTTCATAAAATAGATACCCGCCACCATAAACATCAGTAACAGCAAGACTTCAAGATTTGCCGCAACCTCTTCACGGACGTGTTCCGCGCTGGTCATg                                                                                               >  1:405359/1‑139 (MQ=255)                        aaTGCTTAACAGCAAACGGGTAAATATGAACAGCAACAGCTGTTTCATAAAATAGATACCCGCCACCATAAACATCAGTAACAGCAAGACTTCAAGATTTGCCGCAACCTCTTCACGGACGTGTTCCGCGCTGGTCATg                                                                                               <  2:405359/139‑1 (MQ=255)                               aaCAGCAAACGGGTAAATATGAACAGCAACAGCTGTTTCATAAAATAGATCCCCGCCACCATAAACATCAGTAACAGCAAGACTTCAAGATTTGCCGCAACCTCTTCACGGACGTGTTCCGCGCTGGTCATGCCGATGaa                                                                                       <  2:889686/140‑1 (MQ=255)                                 cAGCAAACGGGTAAATATGAACAGCAACAGCTGTTTCATAAAATAGATACCCGCCACCATAAACATCAGTAACAGCAAGACTTCAAGATTTGCCGCAACCTCTTCACGGACGTGTTCCGCGCTGGTCATGCCGATGAATa                                                                                     <  2:621583/140‑1 (MQ=255)                                    cAAACGGGTAAATATGAACAGCAACAGCTGTTTCATAAAATAGATACCCGCCACCATAAACATCAGTAACAGCAAGACTTCAAGATTTGCCGCAACCTCTTCACGGACGTGTTCCGCGCTGGTCATGCCGATGAATACCg                                                                                  <  1:153534/140‑1 (MQ=255)                                      aaCGGGTAAATATGAACAGCAACAGCTGTTTCATAAAATAGATACCCGCCACCATAAACATCAGTAACAGCAAGACTTCAAGATTTGCCGCAACCTCTTCACGGACGTGTTCCGCGCTGGTCATGCCGATGAATACCGCt                                                                                >  1:160827/1‑140 (MQ=255)                                                   gAACAGCAACAGCTGTTTCATAAAATAGATACCCGCCACCATAAACATCAGTAACAGCAAGACTTCAAGATTTGCCGCAACCTCTTCACGGACGTGTTCCGCGCTGGTCATGCCGATGAATACCGCTTCGATAGCCAACa                                                                   <  2:73541/140‑1 (MQ=255)                                                     acagcaacagcTGTTTCATAAAATAGATACCCGCCACCATAAACATCAGTAACAGCAAGACTTCAAGATTTGCCGCAACCTCTTCACGGACGTGTTCCGCGCTGGTCATGCCGATGAATACCGCTTCGATAGCCAACAGa                                                                 >  1:165803/1‑140 (MQ=255)                                                     acagcaacagcTGTTTCATAAAATAGATACCCGCCACCATAAACATCAGTAACAGCAAGACTTCAAGATTTGCCGCAACCTCTTCACGGACGTGTTCCGCGCTGGTCATGCCGATGAATACCGCTTCGATAGCCAACAGa                                                                 <  2:165803/140‑1 (MQ=255)                                                      cagcaacagcTGTTTCATAAAATAGATACCCGCCACCATAAACATCAGTAACAGCAAGACTTCAAGATTTGCCGCAACCTCTTCACGGACGTGTTCCGCGCTGGTCATGCCGATGAATACCGCTTCGATAGCCAACa                                                                   >  1:295060/1‑137 (MQ=255)                                                      cagcaacagcTGTTTCATAAAATAGATACCCGCCACCATAAACATCAGTAACAGCAAGACTTCAAGATTTGCCGCAACCTCTTCACGGACGTGTTCCGCGCTGGTCATGCCGATGAATACCGCTTCGATAGCCAACa                                                                   <  2:295060/137‑1 (MQ=255)                                                      cagcaacagcTGTTTCATAAAATAGATACCCGCCACCATAAACATCAGTAACAGCAAGACTTCAAGATTTGCCGCAACCTCTTCACGGACGTGTTCCGCGCTGGTCATGCCGATGAATACCGCTTCGATAGCCAACAGac                                                                <  2:436363/140‑1 (MQ=255)                                                         caacagcTGTTTCATAAAATAGATACCCGCCACCATAAACATCAGTAACAGCAAGACTTCAAGATTTGCCGCAACCTCTTCACGGACGTGTTCCGCGCTGGTCATGCCGATGAATACCGCTTCg                                                                             >  2:116908/1‑124 (MQ=255)                                                         caacagcTGTTTCATAAAATAGATACCCGCCACCATAAACATCAGTAACAGCAAGACTTCAAGATTTGCCGCAACCTCTTCACGGACGTGTTCCGCGCTGGTCATGCCGATGAATACCGCTTCg                                                                             <  1:116908/124‑1 (MQ=255)                                                              gcTGTTTCATAAAATAGATACCCGCCACCATAAACATCAGTAACAGCAAGACTTCAAGATTTGCCGCAACCTCTTCACGGACGTGTTCCGCGCTGGTCATGCCGATGAATACCGCTTCGATAGCCAACAGACCACCggg                                                         <  1:135933/139‑1 (MQ=255)                                                              gcTGTTTCATAAAATAGATACCCGCCACCATAAACATCAGTAACAGCAAGACTTCAAGATTTGCCGCAACCTCTTCACGGACGTGTTCCGCGCTGGTCATGCCGATGAATACCGCTTCGATAGCCAACAGACCACCggg                                                         >  2:135933/1‑139 (MQ=255)                                                                     aaTAAAATAGATACCCGCCACCATAAACATCAGTAACAGCAAGACTTCAAGATTTGCCGCAACCTCTTCACGGACGTGTTCCGCGCTGGTCATGCCGATGAATACCGCTTCGATAGCCAACAGACCACCGGGGAGCAGCg                                                 <  2:392825/139‑1 (MQ=255)                                                                       tAAAATAGATACCCGCCACCATAAACATCAGTAACAGCAAGACTTCAAGATTTGCCGCAACCTCTTCACGGACGTGTTCCGCGCTGGTCATGCCGATGAATACCGCTTCGATAGCCAACAGACCACCGGGGAGCAGCggg                                               >  1:238023/1‑140 (MQ=255)                                                                                             aaaCATCAGTAACAGCAAGACTTCAAGATTTGCCGCAACCTCTTCACGGACGTGTTCCGCGCTGGTCATGCCGATGAATACCGCTTCGATAGCCAACAGACCACCGGGGTGCAGCGGGTAGCATTTCAGGGCCATCGCCa                         >  2:491265/1‑140 (MQ=255)                                                                                             aaaCATCAGTAACAGCAAGACTTCAAGATTTGCCGCAACCTCTTCACGGACGTGTTCCGCGCTGGTCATGCCGATGAATACCGCTTCGATAGCCAACAGACCACCGGGGAGCAGCGGGTAGCATTTCAGGGCCATCGCCa                         <  2:206185/140‑1 (MQ=255)                                                                                              aaCATCAGTAACAGCAAGACTTCAAGATTTGCCGCAACCTCTTCACGGACGTGTTCCGCGCTGGTCATGCCGATGAATACCGCTTCGATAGCCAACAGACCACCGGGGAGCAGCGGGTAGCATTTCAGGGCCATCGCCag                        <  2:554028/140‑1 (MQ=255)                                                                                              aaCATCAGTAACAGCAAGACTTCAAGATTTGCCGCAACCTCTTCACGGACGTGTTCCGCGCTGGTCATGCCGATGAATACCGCTTCGATAGCCAACAGACCACCGGGGAGCAGCGGGAAGCATTTCAGGGCCATCGCCag                        >  1:839675/1‑140 (MQ=255)                                                                                                 aTCAGTAACAGCAAGACTTCAAGATTTGCCGCAACCTCTTCACGGACGTGTTCCGCGCTGGTCATGCCGATGAATACCGCTTCGATAGCCAACAGACCACCGGGGAGCAGCGGGTAGCATTTCAGGGc                                 >  2:179906/1‑128 (MQ=255)                                                                                                 aTCAGTAACAGCAAGACTTCAAGATTTGCCGCAACCTCTTCACGGACGTGTTCCGCGCTGGTCATGCCGATGAATACCGCTTCGATAGCCAACAGACCACCGGGGAGCAGCGGGTAGCATTTCAGGGc                                 <  1:179906/128‑1 (MQ=255)                                                                                                        aCAGCAAGACTTCAAGATTTGCCGCAACCTCTTCACGGACGTGTTCCGCGCTGGTCATGCCGATGAATACCGCTTCGATAGCCAACAGACCACCGGGGAGCAGCGGGTAGCATTTCAGGGCCATCGCCAGAGTGAaaata              >  1:64479/1‑140 (MQ=255)                                                                                                            cAAGACTTCAAGATTTGCCGCAACCTCTTCACGGACGTGTTCCGCGCTGGTCATGCCGATGAATACCGCTTCGATAGCCAACAGACCACCGGGGAGCAGCGGGTAGCATTTCAGGGCCATCGCCAGAGTGAAAATAAAtt          >  2:839021/1‑140 (MQ=255)                                                                                                             aaGACTTCAAGATTTGCCGCAACCTCTTCACGGACGTGTTCCGCGCTGGTCATGCCGATGAATACCGCTTCGATAGCCAACAGACCACCGGGGAGCAGCGGGTAGCATTTCAGGGCCATCGCCAGAGTGAAAATAAATTc         >  1:630634/1‑140 (MQ=255)                                                                                                                aCTTCAAGATTTGCCGCAACCTCTTCACGGACGTGTTCCGCGCTGGTCATGCCGATGAATACCGCTTCGATAGCCAACAGACCACCGGGGAGCAGCGGGTAGCATTTCAGGGCCATCGCCAGAGTGAAAATAAATTCCGc      >  1:298805/1‑140 (MQ=255)                                                                                                                    cAAGATTTGCCGCAACCTCTTCACGGACGTGTTCCGCGCTGGTCATGCCGATGAATACCGCTTCGATAGCCAACAGACCACCGGGGAGCAGCGGGTAGCATTTCAGGGCCATCGCCAGAGTGAAAATAAATTCCGCTAcc  <  2:630634/140‑1 (MQ=255)                                                                                                                    cAAGATTTGCCGCAACCTCTTCACGGACGTGTTCCGCGCTGGTCATGCCGATGAATACCGCTTCGATAGCCAACAGACCACCGGGGAGCAGCGGGTAGCATTTCAGGGCCATCGCCAGAGTGAAAATAAATTCCGCTAcc  >  2:98174/1‑140 (MQ=255)                                                                                                                                 |                                                                                                                               AGCGACAGCAGCATTTTGGAGCGAATGCTTAACAGCAAACGGGTAAATATGAACAGCAACAGCTGTTTCATAAAATAGATACCCGCCACCATAAACATCAGTAACAGCAAGACTTCAAGATTTGCCGCCACCTCTTCACGGACGTGTTCCGCGCTGGTCATGCCGATGAATACCGCTTCGATAGCCAACAGACCACCGGGGAGCAGCGGGTAGCATTTCAGGGCCATCGCCAGAGTGAAAATAAATTCCGCTACC  >  NC_000913/1234323‑1234577

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑