| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,206,435 | 0 | C | T | 32.1% | 36.9 / 14.6 | 28 | intergenic (‑179/‑28) | ttdR/ttdA | DNA‑binding transcriptional activator Dan/L(+)‑tartrate dehydratase subunit alpha |
| Reads supporting (aligned to +/- strand): ref base C (0/19); new base T (9/0); total (9/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.45e-07 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATG‑AGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTG > NC_000913/3206307‑3206564 | ccATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAc < 1:715770/140‑1 (MQ=255) ttCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACaaa < 2:349214/140‑1 (MQ=255) tctGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAg < 2:337211/140‑1 (MQ=255) tctGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAg < 2:382507/140‑1 (MQ=255) tctGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAg < 1:475532/140‑1 (MQ=255) gcgcAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAAAAAAAAAAGGAGTTGc > 2:475532/1‑140 (MQ=255) ttcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATG‑AGCGa < 2:809630/140‑1 (MQ=255) tttCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATG‑AGCGAAAGtaat < 1:557444/140‑1 (MQ=255) tttCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATG‑AGCGAAAGtaat < 1:615663/140‑1 (MQ=255) aTTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATG‑AGCGAAAGTAATAAGCAACAgg < 1:180183/140‑1 (MQ=255) ttCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATG‑AGCGAAAGTAATAAGCAACAGGc < 2:281934/140‑1 (MQ=255) ttCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATG‑AGCGAAAGTAATAAGCAACAGGc < 1:125342/140‑1 (MQ=255) tCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATG‑AGCGAAAGTAATAAGCAACAGGCa < 2:396570/140‑1 (MQ=255) aaCTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATG‑AGCGAAAGTAATAAGCAACAGGCAGTGaa < 2:104314/140‑1 (MQ=255) ttAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGGATTTTTTCCGACAAAAACAAAAAATAGAGATGCCCTGTTA‑AGGGGAAGGAATAAGAAAAAAGCAGGGAATaaat > 2:358269/1‑138 (MQ=255) tACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATG‑AGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACa < 2:777787/140‑1 (MQ=255) gaCAGATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATG‑AGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACa < 1:477407/139‑1 (MQ=255) aCGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAAAAAAAAAAGGAGGTGGCATGATG‑AGGGAAAGAAAAAAACAACAAGCAGAGAATAAATTGACAGAGATTGtt > 2:232774/1‑139 (MQ=255) ggTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAAAAAAAAAAAAAAAATGGAGTTGCCATGGTG‑AGCGAAAAAAAAAAACAAAAAGCAAGGAATAAGATGACAGAGAATGTCGc > 2:286207/1‑140 (MQ=255) gCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCAGACAAAAAAAAAAAATGGAGTTGGCCTGGTGAAGGGAAAG‑AAAAAACAAAAAGCAATGAAAAAGATGACAGAAAATTTCGCTAAc > 2:88682/1‑140 (MQ=255) cAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCAGAAAAAAAAAAAAAATGGGGGTGCCATGATG‑AGGGGAAAGAATAAAAAACAAGGAATGAAAAAGTTGAAAGAGATTGTTGcctgtt > 2:477407/1‑135 (MQ=255) ataAACCCACATTTTTAGTGGTAGTTTGTGGCGGTAAATTTTTCCAGAAAAATAAAAAAACGGGAGTTGCCATGATG‑AGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGcc < 1:88682/140‑1 (MQ=255) caTTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCCGACAAAAACAAAAAATGGGGTTGGCAAGATG‑AGGGGAAAAAATAAACAAAAAGGAGTGAATAAATTGACAGAAGTTGTCGCTAACTTTTTCCCCATGAttttt > 2:300297/1‑138 (MQ=255) caTTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCAAACAAAAACAAAAAACAGGGTTGGCCCGATG‑AGCGGAAGGAAAAAGAAAAAAGCAGGGAATAAATTGACAGAGAATGTTGCTAACTTTTACCCCATGAatttt > 2:518204/1‑138 (MQ=255) tttAGTGGTAGTTTGTGGCGGTGAAATTTTTCAGACAAAAAAAAAAAATGGAGGTGCCATGATG‑AGGGGAAGTAATAAAAAAAAAGCAAGGAAAAAGTTGACAGAGATTGTCGCTAAATTTACCGCCATGATTTTTAccc > 2:233096/1‑140 (MQ=255) tGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATG‑AGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATg < 1:300297/140‑1 (MQ=255) ggTAGTTTGTGGCGGTGAATTTTTTCTAACAAAAACAAAAAATCGAGTTTACATGAAG‑AACGAAAGGAATAAAAAAAAAGAAGGGAAAAAATTGAAAAAGATTGTCGCTTAATTTACCGCCACAATTTTTTCCCGAATgg > 2:602685/1‑139 (MQ=255) gtgGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATG‑AGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGAc < 1:763273/140‑1 (MQ=255) gCGGTGAATTTTTCCAGACAAATAAAAAAACTGGAGTTGCCATGATG‑AGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACgtg < 1:232774/140‑1 (MQ=255) gCGGTAAATTTTCCCAGAAAAATAAAAAAACTGGAGTTCCAAGGATG‑AGCAAAAGTAATAAGCAAAAGGCAGTGAATAAGTGAACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACgtg < 2:284514/140‑1 (MQ=255) gTGAATTTTTTCCGACAAAAACAAAAAATGGAGGTGGCATGGTG‑AGGGAAAATAATAAACAACAAGCAGTGAATAAAGTGACAAAGATTGTCGCTAACTTTACCGCCCTGATTTCTACCCGAATGCCTGATGACgtgggg > 2:71539/1‑138 (MQ=255) gTGAATTTTTTCAGACAAAAACAAAAAATGGAGGTGGCATGATG‑AGGGAAAGTAATAAGCAACAGGCAGTGAAAAAGTTGACAGAGATTGTCGCTAACCTTACCGCCCTGATTTTTTCCCGAATGCCTGATGACgtggtg > 2:744572/1‑140 (MQ=255) | CCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATG‑AGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTG > NC_000913/3206307‑3206564 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |