Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	205,971	G→T	P282P (CCG→CCT)	dnaE →	DNA polymerase III subunit alpha

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	205,971	0	G	T	100.0%	176.5 / NA	52	P282P (CCG→CCT)	dnaE	DNA polymerase III subunit alpha
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (26/26);  total (26/26)

AGCAATATATGCGTAGCGAAGAGGAGATGTGTGAGCTGTTTGCCGACATCCCCGAAGCCCTTGCCAACACCGTTGAGATCGCCAAACGCTGTAACGTAACCGTGCGTCTTGGTGAATACTTCCTGCCGCAGTTCCCGACCGGGGACATGAGCACCGAAGATTATCTGGTCAAGCGTGCAAAAGAGGGCCTGGAAGAGCGTCTGGCCTTTTTATTCCCTGATGAGGAAGAACGTCTTAAGCGCCGCCCGGAATATGACGAACGTCTGGAGACTG  >  NC_000913/205835‑206107                                                                                                                                         |                                                                                                                                         agcaATATATGCGTAGCGAAGAGGAGATGTGTGAGCTGTTTGCCGACATCCCCGAAGCCCTTGCCAACACCGTTGAGATCGCCAAACGCTGTAACGTAACCGTGCGTCTTGGTGAATACTTCCTGCCGCAGTTCCCTAcc                                                                                                                                       >  2:714073/1‑140 (MQ=255)    aaTATATGCGTAGCGAAGAGGAGATGTGTGAGCTGTTTGCCGACATCCCCGAAGCCCTTGCCAACACCGTTGAGATCGCCAAACGCTGTAACGTAACCGTGCGTCTTGGTGAATACTTCCTGCCGCAGTTCCCTACCggg                                                                                                                                    <  1:353321/140‑1 (MQ=255)             gTAGCGAAGAGGAGATGTGTGAGCTGTTTGCCGACATCCCCGAAGCCCTTGCCAACACCGTTGAGATCGCCAAACGCTGTAACGTAACCGTGCGTCTTGGTGAATACTTCCTGCCGCAGTTCCCTACCGGGGACATGAGc                                                                                                                           >  1:777607/1‑140 (MQ=255)               aGCGAAGAGGAGATGTGTGAGCTGTTTGCCGACATCCCCGAAGCCCTTGCCAACACCGTTGAGATCGCCAAACGCTGTAACGTAACCGTGCGTCTTGGTGAATACTTCCTGCCGCAGTTCCCTACCGGGGACATGa                                                                                                                             >  1:22554/1‑136 (MQ=255)               aGCGAAGAGGAGATGTGTGAGCTGTTTGCCGACATCCCCGAAGCCCTTGCCAACACCGTTGAGATCGCCAAACGCTGTAACGTAACCGTGCGTCTTGGTGAATACTTCCTGCCGCAGTTCCCTACCGGGGACATGa                                                                                                                             <  2:22554/136‑1 (MQ=255)                gCGAAGAGGAGATGTGTGAGCTGTTTGCCGACATCCCCGAAGCCCTTGCCAACACCGTTGAGATCGCCAAACGCTGTAACGTAACCGTGCGTCTTGGTGAATACTTCCTGCCGCAGTTCCCTACCGGGGACATGAGCAcc                                                                                                                        <  1:188097/140‑1 (MQ=255)                gCGAAGAGGAGATGTGTGAGCTGTTTGCCGACATCCCCGAAGCCCTTGCCAACACCGTTGAGATCGCCAAACGCTGTAACGTAACCGTGCGTCTTGGTGAATACTTCCTGCCGCAGTTCCCTACCGGGGACATGAGCAcc                                                                                                                        >  1:311261/1‑140 (MQ=255)                gCGAAGAGGAGATGTGTGAGCTGTTTGCCGACATCCCCGAAGCCCTTGCCAACACCGTTGAGATCGCCAAACGCTGTAACGTAACCGTGCGTCTTGGTGAATACTTCCTGCCGCAGTTCCCTACCGGGGACATGAGCAcc                                                                                                                        <  2:741867/140‑1 (MQ=255)                gCGAAGAGGAGATGTGTGAGCTGTTTGCCGACATCCCCGAAGCCCTTGCCAACACCGTTGAGATCGCCAAACGCTGTAACGTAACCGTGCGTCTTGGTGAATACTTCCTGCCGCAGTTCCCTACCGGGGACATGAGCAcc                                                                                                                        >  1:90806/1‑140 (MQ=255)                                 gAGCTGTTTGCCGACATCCCCGAAGCCCTTGCCAACACCGTTGAGATCGCCAAACGCTGTAACGTAACCGTGCGTCTTGGTGAATACTTCCTGCCGCAGTTCCCTACCGGGGACATGAGCACCGAAGATTATCTGGTCaa                                                                                                       <  2:777607/140‑1 (MQ=255)                                  aGCTGTTTGTCGACATCCCCGAAGCCCTTGCCAACACCGTTGAGATCGCCAAACGCTGTAACGTAACCGTGCGTCTTGGTGAATACTTCCTGCCGCAGTTCCCTACCGGGGACATGAGCACCGAAGATTATCTGGTCAAg                                                                                                      >  2:526993/1‑140 (MQ=255)                                             gACATCCCCGAAGCCCTTGCCAACACCGTTGAGATCGCCAAACGCTGTAACGTAACCGTGCGTCTTGGTGAATACTTCCTGCCGCAGTTCCCTACCGGGGACATGAGCACCGAAGATTATCTGGTCAAGCGTGCAAAaga                                                                                           <  2:491803/140‑1 (MQ=255)                                                 tCCCCGAAGCCCTTGCCAACACCGTTGAGATCGCCAAACGCTGTAACGTAACCGTGCGTCTTGGTGAATACTTCCTGCCGCAGTTCCCTACCGGGGACATGAGCACCGAAGATTATCTGGTCAAGCGTGCAAAAGAGGGc                                                                                       <  1:641100/140‑1 (MQ=255)                                                 tCCCCGAAGCCCTTGCCAACACCGTTGAGATCGCCAAACGCTGTAACGTAACCGTGCGTCTTGGTGAATACTTCCTGCCGCAGTTCCCTACCGGGGACATGAGCACCGAAGATTATCTGGTCAAGCGTGCAAAAGAGGGc                                                                                       >  1:638489/1‑140 (MQ=255)                                                 tCCCCGAAGCCCTTGCCAACACCGTTGAGATCGCCAAACGCTGTAACGTAACCGTGCGTCTTGGTGAATACTTCCTGCCGCAGTTCCCTACCGGGGACATGAGCACCGAAGATTATCTGGTCAAGCGTGCAAAAGAGGGc                                                                                       >  2:151657/1‑140 (MQ=255)                                                          cccTTGCCAACACCGTTGAGATCGCCAAACGCTGTAACGTAACCGTGCGTCTTGGTGAATACTTCCTGCCGCAGTTCCCTACCGGGGACATGAGCACCGAAGATTATCTGGTCAAGCGTGCAAAAGAGGGCCTGGAagag                                                                              >  2:178494/1‑140 (MQ=255)                                                            cTTGCCAACACCGTTGAGATCGCCAAACGCTGTAACGTAACCGTGCGTCTTGGTGAATACTTCCTGCCGCAGTTCCCTACCGGGGACATGAGCACCGAAGATTATCTGGTCAAGCGTGCAAAAGAGGGCCTGGAAGATCg                                                                            >  2:772652/1‑140 (MQ=255)                                                            cTTGCCAACACCGTTGAGATCGCCAAACGCTGTAACGTAACCGTGCGTCTTGGTGAATACTTCCTGCCGCAGTTCCCTACCGGGGACATGAGCACCGAAGATTATCTGGTCAAGCGTGCAAAAGAGGGCCTGGAAGAGCg                                                                            >  1:353222/1‑140 (MQ=255)                                                              tGCCAACACCGTTGAGATCGCCAAACGCTGTAACGTAACCGTGCGTCTTGGTGAATACTTCCTGCCGCAGTTCCCTACCGGGGACATGAGCACCGAAGATTATCTGGTCAAGCGTGCAAAAGAGGGCCTGGAAGAGCGTc                                                                          <  1:791054/140‑1 (MQ=255)                                                                 cAACACCGTTGAGATCGCCAAACGCTGTAACGTAACCGTGCGTCTTGGTGAATACTTCCTGCCGCAGTTCCCTACCGGGGACATGAGCACCGAAGATTATCTGGTCAAGCGTGCAAAAGAGGGCCTGGAAGATCGTCTgg                                                                       <  1:772652/140‑1 (MQ=255)                                                                 cAACACCGTTGAGATCGCCAAACGCTGTAACGTAACCGTGCGTCTTGGTGAATACTTCCTGCCGCAGTTCCCTACCGGGGACATGAGCACCGAAGATTATCTGGTCAAGCGTGCAAAAGAGGGCCTGGAAGAGCGTCTgg                                                                       <  2:638489/140‑1 (MQ=255)                                                                 cAACACCGTTGAGATCGCCAAACGCTGTAACGTAACCGTGCGTCTTGGTGAATACTTCCTGCCGCAGTTCCCTACCGGGGACATGAGCACCGAAGATTATCTGGTCAAGCGTGCAAAAGAGGGCCTGGAAGAGCGTCTgg                                                                       <  2:353222/140‑1 (MQ=255)                                                                   acacCGTTGAGATCGCCAAACGCTGTAACGTAACCGTGCGTCTTGGTGAATACTTCCTGCCGCAGTTCCCTACCGGGGACATGAGCACCGAAGATTATCTGGTCAAGCGTGCAAAACAGGGCCTGGAAGAGCGTCTGGcc                                                                     <  1:714073/140‑1 (MQ=255)                                                                    cacCGTTGAGATCGCCAAACGCTGTAACGTAACCGTGCGTCTTGGTGAATACTTCCTGCCGCAGTTCCCTACCGGGGACATGAGCACCGAAGATTATCTGGTCAAGCGTGCAAAAGAGGGCCTGGAAGAGCGTCTGGCCt                                                                    >  2:772439/1‑140 (MQ=255)                                                                       cGTTGAGATCGCCAAACGCTGTAACGTAACCGTGCGTCTTGGTGAATACTTCCTGCCGCAGTTCCCTACCGGGGACATGAGCACCGAAGATTATCTGGTCAAGCGTGCAAAAGAGGGCCTGGAAGAGCGTCTGGCCtttt                                                                 >  1:372576/1‑140 (MQ=255)                                                                         ttGAGATCGCCAAACGCTGTAACGTAACCGTGCGTCTTGGTGAATACTTCCTGCCGCAGTTCCCTACCGGGGACATGAGCACCGAAGATTATCTGGTCAAGCGTGCAAAAGAGGGCCTGGAAGAGCGTCTGGCCTTTTTa                                                               >  1:571909/1‑140 (MQ=255)                                                                         ttGAGATCGCCAAACGCTGTAACGTAACCGTGCGTCTTGGTGAATACTTCCTGCCGCAGTTCCCTACCGGGGACATGAGCACCGAAGATTATCTGGTCAAGCGTGCAAAAGAGGGCCTGGAAGAGCGTCTGGCCTTTTTa                                                               >  1:647602/1‑140 (MQ=255)                                                                             gaTCGCCAAACGCTGTAACGTAACCGTGCGTCTTGGTGAATACTTCCTGCCGCAGTTCCCTACCGGGGACATGAGCACCGAAGATTATCTGGTCAAGCGTGCAAAAGAGGGCCTGGAAGAGCGTCTGGCCTTTTTATTcc                                                           <  2:571909/140‑1 (MQ=255)                                                                              aTCGCCAAACGCTGTAACGTAACCGTGCGTCTTGGTGAATACTTCCTGCCGCAGTTCCCTACCGGGGACATGAGCACCGAAGATTATCTGGTCAAGCGTGCAAAAGAGGGCCTGGAAGAGCGTCTGGCCTTTTTATTccc                                                          <  2:372576/140‑1 (MQ=255)                                                                                  ccAAACGCTGTAACGTAACCGTGCGTCTTGGTGAATACTTCCTGCCGCAGTTCCCTACCGGGGACATGAGCACCGAAGATTATCTGGTCAAGCGTGCAAAAGAGGGCCTGGAAGAGCGTCTGGCCTTTTTATTCCCtgat                                                      <  1:151657/140‑1 (MQ=255)                                                                                   cAAACGCTGTAACGTAACCGTGCGTCTTGGTGAATACTTCCTGCCGCAGTTCCCTACCGGGGACATGAGCACCGAAGATTATCTGGTCAAGCGTGCAAAAGAGGGCCTGGAAGAGCGTCTgg                                                                       <  2:828694/122‑1 (MQ=255)                                                                                   cAAACGCTGTAACGTAACCGTGCGTCTTGGTGAATACTTCCTGCCGCAGTTCCCTACCGGGGACATGAGCACCGAAGATTATCTGGTCAAGCGTGCAAAAGAGGGCCTGGAAGAGCGTCTgg                                                                       >  1:828694/1‑122 (MQ=255)                                                                                   cAAACGCTGTAACGTAACCGTGCGTCTTGGTGAATACTTCCTGCCGCAGTTCCCTACCGGGGACATGAGCACCGAAGATTATCTGGTCAAGCGTGCAAAAGAGGGCCTGGAAGAGCGTCTGGCCTTTTTATTCCCtgatg                                                     <  1:178494/140‑1 (MQ=255)                                                                                   cAAACGCTGTAACGTAACCGTGCGTCTTGGTGAATACTTCCTGCCGCAGTTCCCTACCGGGGACATGAGCACCGAAGATTATCTGGTCAAGCGTGCAAAAGAGGGCCTGGAAGAGCGTCTGGCCTTTTTATTCCCtgatg                                                     <  1:526993/140‑1 (MQ=255)                                                                                   cAAACGCTGTAACGTAACCGTGCGTCTTGGTGAATACTTCCTGCCGCAGTTCCCTACCGGGGACATGAGCACCGAAGATTATCTGGTCAAGCGTGCAAAAGAGGGCCTGGAAGAGCGTCTGGCCTTTTTATTCCCtgatg                                                     <  1:772439/140‑1 (MQ=255)                                                                                          tGTAACGTAACCGTGCGTCTTGGTGAATACTTCCTGCCGCAGTTCCCTACCGGGGACATGAGCACCGAAGATTATCTGGTCAAGCGTGCAAAAGAGGGCCTGGAAGAGCGTCTGGCCTTTTTATTCCCTGATGAGgaaga                                              >  1:195122/1‑140 (MQ=255)                                                                                                 taacCGTGCGTCTTGGTGAATACTTCCTGCCGCAGTTCCCTACCGGGGACATGAGCACCGAAGATTATCTGGTCAAGCGTGCAAAAGAGGGCCTGGAAGAGCGTCTGGCCTTTTTATTCCCtgatg                                                     >  2:103581/1‑126 (MQ=255)                                                                                                 taacCGTGCGTCTTGGTGAATACTTCCTGCCGCAGTTCCCTACCGGGGACATGAGCACCGAAGATTATCTGGTCAAGCGTGCAAAAGAGGGCCTGGAAGAGCGTCTGGCCTTTTTATTCCCtgatg                                                     <  1:103581/126‑1 (MQ=255)                                                                                                    ccGTGCGTCTTGGTGAATACTTCCTGCCGCAGTTCCCTACCGGGGACATGAGCACCGAAGATTATCTGGTCAAGCGTGCAAAAGAGGGCCTGGAAGAGCGTCTGGCCTTTTTATTCCCTGATGAGGAAGAACGTCTTAAg                                    >  2:349581/1‑140 (MQ=255)                                                                                                      gTGCGTCTTGGTGAATACTTCCTGCCGCAGTTCCCTACCGGGGACATGAGCACCGAAGATTTTCTGGTCAAGCGTGCAAAAGAGGGCCTGGAAGAGCGTCTGGCCTTTTTATTCCCTGATGAGGAAGAACGTCTTAAgcg                                  >  1:365346/1‑140 (MQ=255)                                                                                                      gTGCGTCTTGGTGAATACTTCCTGCCGCAGTTCCCTACCGGGGACATGAGCACCGAAGATTATCTGGTCAAGCGTGCAAAAGAGGGCCTGGAAGAGCGTCTGGCCTTTTTATTCCCTGATGAGGAAGAACGTCTTAAgcg                                  <  2:195122/140‑1 (MQ=255)                                                                                                       tGCGTCTTGGTGAATACTTCCTGCCGCAGTTCCCTACCGGGGACATGAGCACCGAAGATTATCTGGTCAAGCGTGCAAAAGAGGGCCTGGAAGAGCGTCTGGCCTTTTTATTCCCTGATGAGGAAGAACGTCTTAAgcgc                                 >  1:583746/1‑140 (MQ=255)                                                                                                         cGTCTTGGTGAATACTTCCTGCCGCAGTTCCCTACCGGGGACATGAGCACCGAAGATTATCTGGTCAAGCGTGCAAAAGAGGGCCTGGAAGAGCGTCTGGCCTTTTTATTCCCTGATGAGGAAGAACGTCTTAAGcgccg                               >  1:727623/1‑140 (MQ=255)                                                                                                         cGTCTTGGTGAATACTTCCTGCCGCAGTTCCCTACCGGGGACATGAGCACCGAAGATTATCTGGTCAAGCGTGCAAAAGAGGGCCTGGAAGAGCGTCTGGCCTTTTTATTCCCTGATGAGGAAGAACGTCTTAAGcgccg                               >  2:248030/1‑140 (MQ=255)                                                                                                             ttGGTGAATACTTCCTGCCGCAGTTCCCTACCGGGGACATGAGCACCGAAGATTATCTGGTCAAGCGTGCAAAAGAGGGCCTGGAAGAGCGTCTGGCCTTTTTATTCCCTGATGAGGAAGAACGTCTTAAGCgccgcc                             <  2:437752/138‑1 (MQ=255)                                                                                                             ttGGTGAATACTTCCTGCCGCAGTTCCCTACCGGGGACATGAGCACCGAAGATTATCTGGTCAAGCGTGCAAAAGAGGGCCTGGAAGAGCGTCTGGCCTTTTTATTCCCTGATGAGGAAGAACGTCTTAAGCgccgcc                             >  1:437752/1‑138 (MQ=255)                                                                                                              tGGTGAATACTTCCTGCCGCAGTTCCCTACCGGGGACATGAGCACCGAAGATTTTCTGGTCAAGCGTGCAAAAGAGGGCCTGGAAGAGCGTCTGGCCTTTTTATTCCCTGATGAGGAAGAACGTCTTAAGCGCCGCCCgg                          <  2:365346/140‑1 (MQ=255)                                                                                                                        ttCCTGCCGCAGTTCCCTACCGGGGACATGAGCACCGAAGATTATCTGGTCAAGCGTGCAAAAGAGGGCCTGGAAGAGCGTCTGGCCTTTTTATTCCCTGATGAGGAAGAACGTCTTAAGCGCCGCCCGGAATATGACg                 >  2:484636/1‑139 (MQ=255)                                                                                                                        ttCCTGCCGCAGTTCCCTACCGGGGACATGAGCACCGAAGATTATCTGGTCAAGCGTGCAAAAGAGGGCCTGGAAGAGCGTCTGGCCTTTTTATTCCCTGATGAGGAAGAACGTCTTAAGCGCCGCCCGGAATATGACg                 <  1:484636/139‑1 (MQ=255)                                                                                                                                  aGTTCCCTACCGGGGACATGAGCACCGAAGATTATCTGGTCAAGCGTGCAAAAGAGGGCCTGGAAGAGCGTCTGGCCTTTTTATTCCCTGATGAGGAAGAACGTCTTAAGCGCCGCCCGGAATATGACGAACGTCTGgag      >  2:779403/1‑140 (MQ=255)                                                                                                                                     tCCCTACCGGGGACATGAGCACCGAAGATTATCTGGTCAAGCGTGCAAAAGAGGGTCTGGAAGAGCGTCTGGCCTTTTTATTCCCTGATGAGGAAGAACGTCTTAAGCGCCGCCCGGAATATGACGAACGTCTGGAGACt   <  1:354802/140‑1 (MQ=255)                                                                                                                                      cccTACCGGGGACATGAGCACCGAAGATTATCTGGTCAAGCGTGCAAAAGAGGGCCTGGAAGAGCGTCTGGCCTTTTTATTCCCTGATGAGGAAGAACGTCTTAAGCGCCGCCCGGAATATGACGAACGTCTGGAGACTg  <  2:647602/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                         AGCAATATATGCGTAGCGAAGAGGAGATGTGTGAGCTGTTTGCCGACATCCCCGAAGCCCTTGCCAACACCGTTGAGATCGCCAAACGCTGTAACGTAACCGTGCGTCTTGGTGAATACTTCCTGCCGCAGTTCCCGACCGGGGACATGAGCACCGAAGATTATCTGGTCAAGCGTGCAAAAGAGGGCCTGGAAGAGCGTCTGGCCTTTTTATTCCCTGATGAGGAAGAACGTCTTAAGCGCCGCCCGGAATATGACGAACGTCTGGAGACTG  >  NC_000913/205835‑206107

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑