Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,469,892	G→T	M254I (ATG→ATT)	yfdI →	CPS‑53 (KpLE1) prophage; serotype specific glucosyl transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,469,892	0	G	T	100.0%	181.1 / NA	52	M254I (ATG→ATT)	yfdI	CPS‑53 (KpLE1) prophage; serotype specific glucosyl transferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (29/23);  total (29/23)

ATCTGTAATATGCGTGGTGTGCTTGCTTGCGTCAATATTTGCAATATCTTATTCAAAAGGTGTTGTTGAATTAAATAAGTACCATGCAACATACTTCGGTAGTTATCTTTATATGAAAAACAACGGGTATAAAATGCCATCGTATGTTGATGATAAGTGTGTTGGGTTAGATGCCTGGGGTAATAAATTCGACATATCATTTGGCGCAACCCCAACAGAAGTTGGAACGGAATGTTTCGAATCTCATAAAGATGAAACGTTTTCGAATGCA  >  NC_000913/2469757‑2470027                                                                                                                                        |                                                                                                                                        aTCTGTAATATGCGTGGTGTGCTTGCTTGCGTCAATATTTGCAATATCTTATTCAAAAGGTGTTGTTGAATTAAATAAGTACCATGCAACATACTTCGGTAGTTATCTTTATATGAAAAACAACGGGTATAAAATTCCAt                                                                                                                                     >  1:576210/1‑140 (MQ=255)    tGTAATATGCGTGGTGTGCTTGCTTGCGTCAATATTTGCAATATCTTATTCAAAAGGTGTTGTTGAATTAAATAAGTACCATGCAACATACTTCGGTAGTTATCTTTATATGAAAAACAACGGGTATAAAATTcc                                                                                                                                       >  2:389982/1‑135 (MQ=255)    tGTAATATGCGTGGTGTGCTTGCTTGCGTCAATATTTGCAATATCTTATTCAAAAGGTGTTGTTGAATTAAATAAGTACCATGCAACATACTTCGGTAGTTATCTTTATATGAAAAACAACGGGTATAAAATTCCATCGt                                                                                                                                  <  1:157447/140‑1 (MQ=255)    tGTAATATGCGTGGTGTGCTTGCTTGCGTCAATATTTGCAATATCTTATTCAAAAGGTGTTGTTGAATTAAATAAGTACCATGCAACATACTTCGGTAGTTATCTTTATATGAAAAACAACGGGTATAAAATTCCATCGt                                                                                                                                  <  1:528513/140‑1 (MQ=255)       aaTATGCGTGGTGTGCTTGCTTGCGTCAATATTTGCAATATCTTATTCAAAAGGTGTTGTTGAATTAAATAAGTACCATGCAACATACTTCGGTAGTTATCTTTATATGAAAAACAACGGGTATAAAATTCCATCGTATg                                                                                                                               <  2:373559/140‑1 (MQ=255)       aaTATGCGTGGTGTGCTTGCTTGCGTCAATATTTGCAATATCTTATTCAAAAGGTGTTGTTGAATTAAATAAGTACCATGCAACATACTTCGGTAGTTATCTTTATATGAAAAACAACGGGTATAAAATTCCATCGTATg                                                                                                                               <  2:838831/140‑1 (MQ=255)           tGCGTGGTGTGCTTGCTTGCGTCAATATTTGCAATATCTTATTCAAAAGGTGTTGTTGAATTAAATAAGTACCATGCAACATACTTCGGTAGTTATCTTTATATGAAAAACAACGGGTATAAAATTCCATCGTATGTtga                                                                                                                           >  2:190889/1‑140 (MQ=255)           tGCGTGGTGTGCTTGCTTGCGTCAATATTTGCAATATCTTATTCAAAAGGTGTTGTTGAATTAAATAAGTACCATGCAACATACTTCGGTAGTTATCTTTATATGAAAAACAACGGGTATAAAATTCCATCGTATGTtga                                                                                                                           <  1:559965/140‑1 (MQ=255)            gCGTGGTGTGCTTGCTTGCGTCAATATTTGCAATATCTTATTCAAAAGGTGTTGTTGAATTAAATAAGTACCATGCAACATACTTCGGTAGTTATCTTTATATGAAAAACAACGGGTATAAAATTCCATCGTATGTtgat                                                                                                                          <  1:227866/140‑1 (MQ=255)             cGTGGTGTGCTTGCTTGCGTCAATATTTGCAATATCTTATTCAAAAGGTGTTGTTGAATTAAATAAGTACCATGCAACATACTTCGGTAGTTATCTTTATATGAAAAACAACGGGTATAAAATTCCATCGTATGTtgatg                                                                                                                         >  1:165987/1‑140 (MQ=255)             cGTGGTGTGCTTGCTTGCGTCAATATTTGCAATATCTTATTCAAAAGGTGTTGTTGAATTAAATAAGTACCATGCAACATACTTCGGTAGTTATCTTTATATGAAAAACAACGGGTATAAAATTCCATCGTATGTtgatg                                                                                                                         >  2:64852/1‑140 (MQ=255)             cGTGGTGTGCTTGCTTGCGTCAATATTTGCAATATCTTATTCAAAAGGTGTTGTTGAATTAAATAAGTACCATGCAACATACTTCGGTAGTTATCTTTATATGAAAAACAACGGGTATAAAATTCCATCGTATGTtgatg                                                                                                                         >  1:526750/1‑140 (MQ=255)                  tgtgCTTGCTTGCGTCAATATTTGCAATATCTTATTCAAAAGGTGTTGTTGAATTAAATAAGTACCATGCAACATACTTCGGTAGTTATCTTTATATGAAAAACAACGGGTATAAAATTCCATCGTATGTtgat                                                                                                                          >  1:144250/1‑134 (MQ=255)                  tgtgCTTGCTTGCGTCAATATTTGCAATATCTTATTCAAAAGGTGTTGTTGAATTAAATAAGTACCATGCAACATACTTCGGTAGTTATCTTTATATGAAAAACAACGGGTATAAAATTCCATCGTATGTtgat                                                                                                                          <  2:144250/134‑1 (MQ=255)                     gcttgcttGCGTCAATATTTGCAATATCTTATTCAAAAGGTGTTGTTGAATTAAATAAGTACCATGCAACATACTTCGGTAGTTATCTTTATATGAAAAACAACGGGTATAAAATTCCATCGTATGTTGATGATAAgtgt                                                                                                                 <  1:170455/140‑1 (MQ=255)                       ttgcttgcGTCAATATTTGCAATATCTTATTCAAAAGGTGTTGTTGAATTAAATAAGTACCATGCAACATACTTCGGTAGTTATCTTTATATGAAAAACAACGGGTATAAAATTCCATCGTATGTTGATGATAAgtgtgt                                                                                                               <  2:291321/140‑1 (MQ=255)                       ttgcttgcGTCAATATTTGCAATATCTTATTCAAAAGGTGTTGTTGAATTAAATAAGTACCATGCAACATACTTCGGTAGTTATCTTTATATGAAAAACAACGGGTATAAAATTCCATCGTATGTTGATGATAAgtgtgt                                                                                                               <  2:171759/140‑1 (MQ=255)                        tgcttgcGTCAATATTTGCAATATCTTATTCAAAAGGTGTTGTTGAATTAAATAAGTACCATGCAACATACTTCGGTAGTTATCTTTATATGAAAAACAACGGGTATAAAATTCCATCGTATGTTGATGATAAg                                                                                                                    >  1:411031/1‑134 (MQ=255)                        tgcttgcGTCAATATTTGCAATATCTTATTCAAAAGGTGTTGTTGAATTAAATAAGTACCATGCAACATACTTCGGTAGTTATCTTTATATGAAAAACAACGGGTATAAAATTCCATCGTATGTTGATGATAAg                                                                                                                    <  2:411031/134‑1 (MQ=255)                              cGTCAATATTTGCAATATCTTATTCAAAAGGTGTTGTTGAATTAAATAAGTACCATGCAACATACTTCGGTAGTTATCTTTATATGAAAAACAACGGGTATAAAATTCCATCGTATGTTGATGATAAGTGTGTTGGGTTa                                                                                                        >  1:471554/1‑140 (MQ=255)                                   atatTTGCAATATCTTATTCAAAAGGTGTTGTTGAATTAAATAAGTACCATGCAACATACTTCGGTAGTTATCTTTATATGAAAAACAACGGGTATAAAATTCCATCGTATGTTGATGATAAGTGTGTTGGGTTAGATGc                                                                                                   >  2:791291/1‑140 (MQ=255)                                    tatTTGCAATATCTTATTCAAAAGGTGTTGTTGAATTAAATAAGTACCATGCAACATACTTCGGTAGTTATCTTTATATGAAAAACAACGGGTATAAAATTCCATCGTATGTTGATGATAAGTGTGTTGGGTTAGATGcc                                                                                                  <  1:466114/140‑1 (MQ=255)                                    tatTTGCAATATCTTATTCAAAAGGTGTTGTTGAATTAAATAAGTACCATGCAACATACTTCGGTAGTTATCTTTATATGAAAAACAACGGGTATAAAATTCCATCGTATGTTGATGATAAGTGTGTTGGGTTAGATGcc                                                                                                  <  2:409461/140‑1 (MQ=255)                                                  tATTCAAAAGGTGTTGTTGAATTAAATAAGTACCATGCAACATACTTCGGTAGTTATCTTTATATGAAAAACAACGGGTATAAAATTCCATCGTATGTTGATGATAAGTGTGTTGGGTTAGATGCCTGGGGTAATAAAtt                                                                                    <  1:190889/140‑1 (MQ=255)                                                   aTTCAAAAGGTGTTGTTGAATTAAATAAGTACCATGCAACATACTTCGGTAGTTATCTTTATATGAAAAACAACGGGTATAAAATTCCATCGTATGTTGATGATAAGTGTGTTGGGTTAGATGCCTGGGGTAATAAATTc                                                                                   >  1:361630/1‑140 (MQ=255)                                                         aaGGTGTTGTTGAATTAAATAAGTACCATGCAACATACTTCGGTAGTTATCTTTATATGAAAAACAACGGGTATAAAATTCCATCGTATGTTGATGATAAGTGTGTTGGGTTAGATGCCTGGGGTAATAAATTCg                                                                                  <  2:434476/135‑1 (MQ=255)                                                         aaGGTGTTGTTGAATTAAATAAGTACCATGCAACATACTTCGGTAGTTATCTTTATATGAAAAACAACGGGTATAAAATTCCATCGTATGTTGATGATAAGTGTGTTGGGTTAGATGCCTGGGGTAATAAATTCg                                                                                  >  1:434476/1‑135 (MQ=255)                                                         aaGGTGTTGTTGAATTAAATAAGTACCATGCAACATACTTCGGTAGTTATCTTTATATGAAAAACAACGGGTATAAAATTCCATCGTATGTTGATGATAAGTGTGTTGGGTTAGATGCCTGGGGTAATAAATTCGACata                                                                             >  2:707683/1‑140 (MQ=255)                                                         aaGGTGATGTTGAATTAAATAAGTACCATGCAACATACTTCGGTAGTTATCTTTATATGAAAAACAACGGGTATAAAATTCCATCGTATGTTGATGATAAGTGTGTTGGGTTAGATGCCTGGGGTAATAAATTCGACata                                                                             >  1:48836/1‑140 (MQ=255)                                                               ttgttgAATTAAATAAGTACCATGCAACATACTTCGGTAGTTATCTTTATATGAAAAACAACGGGTATAAAATTCCATCGTATGTTGATGATAAGTGTGTTGGGTTAGATGCCTGGGGTAATAAATTCGACATATCAttt                                                                       >  2:736781/1‑140 (MQ=255)                                                               ttgttgAATTAAATAAGTACCATGCAACATACTTCGGTAGTTATCTTTATATGAAAAACAACGGGTATAAAATTCCATCGTATGTTGATGATAAGTGTGTTGGGTTAGATGCCTGGGGTAATAAATTCGACATATCAttt                                                                       >  2:311069/1‑140 (MQ=255)                                                                    gAATTAAATAAGTACCATGCAACATACTTCGGTAGTTATCTTTATATGAAAAACAACGGGTATAAAATTCCATCGTATGTTGATGATAAGTGTGTTGGGTTAGATGCCTGGGGTAATAAATTCGACATATCATTTGgcgc                                                                  <  1:736781/140‑1 (MQ=255)                                                                               gTACCATGCAACATACTTCGGTAGTTATCTTTATATGAAAAACAACGGGTATAAAATTCCATCGTATGTTGATGATAAGTGTGTTGGGTTAGATGCCTGGGGTAATAAATTCGACATATCATTTGGCGCAAc                                                               <  2:801176/132‑1 (MQ=255)                                                                               gTACCATGCAACATACTTCGGTAGTTATCTTTATATGAAAAACAACGGGTATAAAATTCCATCGTATGTTGATGATAAGTGTGTTGGGTTAGATGCCTGGGGTAATAAATTCGACATATCATTTGGCGCAAc                                                               >  1:801176/1‑132 (MQ=255)                                                                                    aTGCAACATACTTCGGTAGTTATCTTTATATGAAAAACAACGGGTATAAAATTCCATCGTATGTTGATGATAAGTGTGTTGGGTTAGATGCCTGGGGTAATAAATTCGACATATCATTTGGCGCAACCCCAACAGAAGtt                                                  >  2:634618/1‑140 (MQ=255)                                                                                     tGCAACATACTTCGGTAGTTATCTTTATATGAAAAACAACGGGTATAAAATTCCATCGTATGTTGATGATAAGTGTGTTGGGTTAGATGCCTGGGGTAATAAATTCGACATATCATTTGGCGCAACCCCAACAGAAGTTg                                                 >  1:46592/1‑140 (MQ=255)                                                                                     tGCAACATACTTCGGTAGTTATCTTTATATGAAAAACAACGGGTATAAAATTCCATCGTATGTTGATGATAAGTGTGTTGGGTTAGATGCCTGGGGTAATAAATTCGACATATCATTTGGCGCAACCCCAACAGAAGTTg                                                 >  1:784339/1‑140 (MQ=255)                                                                                               ttCGGTAGTTATCTTTATATGAAAAACAACGGGTATAAAATTCCATCGTATGTTGATGATAAGTGTGTTGGGTTAGATGCCTGGGGTAATAAATTCGACATATCATTTGGCGCAACCCCAACAGAAGTTGGAACGGAATg                                       >  1:103987/1‑140 (MQ=255)                                                                                                 cGGTAGTTATCTTTATATGAAAAACAACGGGTATAAAATTCCATCGTATGTTGATGATAAGTGTGTTGGGTTAGATGCCTGGGGTAATAAATTTGACATATCATTTGGCGCAACCCCAACAGAAGTTGGAACGGAATGtt                                     >  1:540857/1‑140 (MQ=255)                                                                                                     aGTTATCTTTATATGAAAAACAACGGGTATAAAATTCCATCGTATGTTGATGATAAGTGTGTTGGGTTAGATGCCTGGGGTAATAAATTCGACATATCATTTGGCGCAACCCCAACAGAAGTTGGAACGGAATGTTTCGa                                 <  2:471554/140‑1 (MQ=255)                                                                                                     aGTTATCTTTATATGAAAAACAACGGGTATAAAATTCCATCGTATGTTGATGATAAGTGTGTTGGGTTAGATGCCTGGGGTAATAAATTCGACATATCATTTGGCGCAACCCCAACAGAAGTTGGAACGGAATGTTTCGa                                 <  1:536126/140‑1 (MQ=255)                                                                                                       ttATCTTTATATGAAAAACAACGGGTATAAAATTCCATCGTATGTTGATGATAAGTGTGTTGGGTTAGATGCCTGGGGTAATAAATTCGACATATCATTTGGCGCAACCCCAACAGAAGTTGGAACGGAATGTTTCGAAt                               <  2:90891/140‑1 (MQ=255)                                                                                                       ttATCTTTATATGAAAAACAACGGGTATAAAATTCCATCGTATGTTGATGATAAGTGTGTTGGGTTAGATGCCTGGGGTAATAAATTCGACATATCATTTGGCGCAACCCCAACAGAAGTTGGAACGGAATGTTTCGAAt                               <  2:70640/140‑1 (MQ=255)                                                                                                             ttATATGAAAAACAACGGGTATAAAATTCCATCGTATGTTGATGATAAGTGTGTTGGGTTAGATGCCTGGGGTAATAAATTCGACATATCATTTGGCGCAACCCCAACAGAAGTTGGAACGGAATGTTTCGAATCTCaca                         <  1:528638/140‑3 (MQ=255)                                                                                                                 atGAAAAACAACGGGTATAAAATTCCATCGTATGTTGATGATAAGTGTGTTGGGTTAGATGCCTGGGGTAATAAATTCGACATATCATTTGGCGCAACCCCAACAGAAGTTGGAACGGAATGTTTCGAATCTCATAAAGa                     >  2:9377/1‑140 (MQ=255)                                                                                                                 atGAAAAACAACGGGTATAAAATTCCATCGTATGTTGATGATAAGTGTGTTGGGTTAGATGCCTGGGGTAATAAATTCGACATATCATTTGGCGCAACCCCAACAGAAGTTGGAACGGAATGTTTCGAATCTCATAAAGa                     >  2:135552/1‑140 (MQ=255)                                                                                                                     aaaaCAACGGGTATAAAATTCCATCGTATGTTGATGATAAGTGTGTTGGGTTAGATGCCTGGGGTAATAAATTCGACATATCATTTGGCGCAACCCCAACAGAAGTTGGAACGGAATGTTTCGAATCTCATAAAGATGaa                 >  1:397403/1‑140 (MQ=255)                                                                                                                        acaacGGGTATAAAATTCCATCGTATGTTGATGATAAGTGTGTTGGGTTAGATGCCTGGGGTAATAAATTCGACATATCATTTGGCGCAACCCCAACAGAAGTTGGAACGGAATGTTTCGAATCTCATAAAGATGAAACg              >  1:500213/1‑140 (MQ=255)                                                                                                                                 ataAAATTCCATCGTATGTTGATGATAAGTGTGTTGGGTTAGATGCCTGGGGTAATAAATTCGACATATCATTTGGCGCAACCCCAACAGAAGTTGGAACGGAATGTTTCGAATCTCATAAAGATGAAACGTTTTCGAAt     >  2:467027/1‑140 (MQ=255)                                                                                                                                 ataAAATTCCATCGTATGTTGATGATAAGTGTGTTGGGTTAGATGCCTGGGGTAATAAATTCGACATATCATTTGGCGCAACCCCAACAGAAGTTGGAACGGAATGTTTCGAATCTCATAAAGATGAAACGTTTTCGAAt     >  1:793129/1‑140 (MQ=255)                                                                                                                                 ataAAATTCCATCGTATGTTGATGATAAGTGTGTTGGGTTAGATGCCTGGGGTAATAAATTCGACATATCATTTGGCGCAACCCCAACAGAAGTTGGAACGGAATGTTTCGAATCTCATAAAGATGAAACGTTTTCGAAt     >  2:28983/1‑140 (MQ=255)                                                                                                                                    aaaTTCCATCGTATGTTGATGATAAGTGTGTTGGGTTAGATGCCTGGGGTAATAAATTTGACATATCATTTGGCGCAACCCCAACAGAAGTTGGAACGGAATGTTTCGAATCTCATAAAGATGAAACGTTTTCGAATGCa  <  2:540857/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                        ATCTGTAATATGCGTGGTGTGCTTGCTTGCGTCAATATTTGCAATATCTTATTCAAAAGGTGTTGTTGAATTAAATAAGTACCATGCAACATACTTCGGTAGTTATCTTTATATGAAAAACAACGGGTATAAAATGCCATCGTATGTTGATGATAAGTGTGTTGGGTTAGATGCCTGGGGTAATAAATTCGACATATCATTTGGCGCAACCCCAACAGAAGTTGGAACGGAATGTTTCGAATCTCATAAAGATGAAACGTTTTCGAATGCA  >  NC_000913/2469757‑2470027

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑