Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,687,211	C→A	P66P (CCG→CCT)	glnB ←	nitrogen regulatory protein PII‑1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,687,211	0	C	A	100.0%	168.8 / NA	48	P66P (CCG→CCT)	glnB	nitrogen regulatory protein PII‑1
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (26/22);  total (26/22)

CGCGTCGTCCTCTTCACCGGTACGGATGCGAATGACCCGTGCCACGTCAAAGACGAAGATTTTACCGTCACCGATTTTGCCGGTTTGCGCCGTGCGAATAATGGTATCGACACAGGTATCGACAATGTCGTCCGGTACGACAATCTCAATTTTCACTTTCGGCAGAAAATCCACCATATACTCCGCGCCGCGGTACAGCTCGGTATGGCCTTTCTGGCGACCAAAGCCTTTCACTTCGGTCACCGTCATGCCGGTAATACCGACTTC  >  NC_000913/2687079‑2687345                                                                                                                                     |                                                                                                                                       cgcgTCGTCCTCTTCACCGGTACGGATGCGAATGACCCGTGCCACGTCAAAGACGAAGATTTTACCGTCACCGATTTTGCCGGTTTGCGCCGTGCGAATAATGGTATCGACACAGGTATCGACAATGTCGTCAGGTACGa                                                                                                                                 >  2:23027/1‑140 (MQ=255) cgcgTCGTCCTCTTCACCGGTACGGATGCGAATGACCCGTGCCACGTCAAAGACGAAGATTTTACCGTCACCGATTTTGCCGGTTTGCGCCGCGCGAATAATGGTATCGACACAGGTATCGACAATGTCGTCAGGTACGa                                                                                                                                 >  2:432240/1‑140 (MQ=255)                       cGGATGCGAATGACCCGTGCCACGTCAAAGACGAAGATTTTACCGTCACCGATTTTGCCGGTTTGCGCCGTGCGAATAATGGTATCGACACAGGTATCGACAATGTCGTCAGGTACGACAATCTCAATTTTCACTTTCgg                                                                                                           >  1:100262/1‑140 (MQ=255)                       cGGATGCGAATGACCCGTGCCACGTCAAAGACGAAGATTTTACCGTCACCGATTTTGCCGGTTTGCGCCGTGCGAATAATGGTATCGACACAGGTATCGACAATGTCGTCAGGTACGACAATCTCAATTTTCACTTTCgg                                                                                                           >  2:182981/1‑140 (MQ=255)                             cGAATGACCCGTGCCACGTCAAAGACGAAGATTTTACCGTCACCGATTTTGCCGGTTTGCGCCGTGCGAATAATGGTATCGACACAGGTATCGACAATGTCGTCAGGTACGACAATCTCAATTTTCACTTTCGGCAGaaa                                                                                                     <  1:362050/140‑1 (MQ=255)                             cGAATGACCCGTGCCACGTCAAAGACGAAGATTTTACCGTCACCGATTTTGCCGGTTTGCGCCGTGCGAATAATGGTATCGACACAGGTATCGACAATGTCGTCAGGTACGACAATCTCAATTTTCACTTTCGGCAGaaa                                                                                                     <  1:479180/140‑1 (MQ=255)                               aaTGACCCGTGCCACGTCAAAGACGAAGATTTTACCGTCACCGATTTTGCCGGTTTGCGCCGTGCGAATAATGGTATCGACACAGGTATCGACAATGTCGTCAGGTACGACAATCTCAATTTTCACTTTCGGCAGAAAAt                                                                                                   <  1:423986/140‑1 (MQ=255)                                 tGACCCGTGCCACGTCAAAGACGAAGATTTTACCGTCACCGATTTTGCCGGTTTGCGCCGTGCGAATAATGGTATCGACACAGGTATCGACAATGTCGTCAGGTACGACAATCTCAATTTTCACTTTCGGCAGAAAATcc                                                                                                 <  2:17396/140‑1 (MQ=255)                                 tGACCCGTGCCACGTCAAAGACGAAGATTTTACCGTCACCGATTTTGCCGGTTTGCGCCGTGCGAATAATGGTATCGACACAGGTATCGACAATGTCGTCAGGTACGACAATCTCAATTTTCACTTTCGGCAGAAAATcc                                                                                                 <  2:100262/140‑1 (MQ=255)                                    cccGTGCCACGTCAAAGACGAAGATTTTACCGTCACCGATTTTGCCGGTTTGCGCCGTGCGAATAATGGTATCGACACAGGTATCGACAATGTCGTCAGGTACGACAATCTCAATTTTCACTTTCGGCAGAAAATccacc                                                                                              >  1:321745/1‑140 (MQ=255)                                    cccGTGCCACGTCAAAGACGAAGATTTTACCGTCACCGATTTTGCCGGTTTGCGCCGTGCGAATAATGGTATCGACACAGGTATCGACAATGTCGTCAGGTACGACAATCTCAATTTTCACTTTCGGCAGAAAATccacc                                                                                              >  2:740195/1‑140 (MQ=255)                                         gCCACGTCAAAGACGAAGATTTTACCGTCACCGATTTTGCCGGTTTGCGCCGTGCGAATAATGGTATCGACACAGGTATCGACAATGTCGTCAGGTACGACAATCTCAATTTTCACTTTCGGCAg                                                                                                        >  1:431626/1‑125 (MQ=255)                                         gCCACGTCAAAGACGAAGATTTTACCGTCACCGATTTTGCCGGTTTGCGCCGTGCGAATAATGGTATCGACACAGGTATCGACAATGTCGTCAGGTACGACAATCTCAATTTTCACTTTCGGCAg                                                                                                        <  2:431626/125‑1 (MQ=255)                                              gTCAAAGACGAAGATTTTACCGTCACCGATTTTGCCGGTTTGCGCCGTGCGAATAATGGTATCGACACAGGTATCGACAATGTCGTCAGGTACGACAATCTCAATTTTCACTTTCGGCAGAAAATCCACCATATACTccg                                                                                    <  1:23027/140‑1 (MQ=255)                                              gTCAAAGACGAAGATTTTACCGTCACCGATTTTGCCGGTTTGCGCCGTGCGAATAATGGTATCGACACAGGTATCGACAATGTCGTCAGGTACGACAATCTCAATTTTCACTTTCGGCAGAAAATCCACCATATACTccg                                                                                    >  1:516336/1‑140 (MQ=255)                                                  aaGACGAAGATTTTACCGTCACCGATTTTGCCGGTTTGCGCCGTGCGAATAATGGTATCGACACAGGTATCGACAATGTCGTCAGGTACGACAATCTCAATTTTCACTTTCGGCAGAAAATCCACCATATACTccgcgcc                                                                                >  1:684963/1‑140 (MQ=255)                                                   aGACGAAGATTTTACCGTCACCGATTTTGCCGGTTTGCGCCGTGCGAATAATGGTATCGACACAGGTATCGACAATGTCGTCAGGTACGACAATCTCAATTTTCACTTTCGGCAGAAAATCCACCATATACTccgcgccg                                                                               <  2:266604/140‑1 (MQ=255)                                                   aGACGAAGATTTTACCGTCACCGATTTTGCCGGTTTGCGCCGTGCGAATAATGGTATCGACACAGGTATCGACAATGTCGTCAGGTACGACAATCTCAATTTTCACTTTCGGCAGAAAATCCACCATATACTccgcgccg                                                                               >  1:230856/1‑140 (MQ=255)                                                      cGAAGATTTTACCGTCACCGATTTTGCCGGTTTGCGCCGTGCGAATAATGGTATCGACACAGGTATCGACAATGTCGTCAGGTACGACAATCTCAATTTTCACTTTCGGCAGAAAATCCACCATATACTCCGCGCCGCgg                                                                            <  2:285256/140‑1 (MQ=255)                                                            ttttACCGTCACCGATTTTGCCGGTTTGCGCCGTGCGAATAATGGTATCGACACAGGTATCGACAATGTCGTCAGGTACGACAATCTCAATTTTCACTTTCGGCAGAAAATCCACCATATACTCCGCGCCGCGGTACAGc                                                                      >  1:510377/1‑140 (MQ=255)                                                                 ccGTCACCGATTTTGCCGGTTTGCGCCGTGCGAATAATGGTATCGACACAGGTATCGACAATGTCGTCAGGTACGACAATCTCAATTTTCACTTTCGGCAGAAAATCCACCATATACTCCGCGCCGCGGTACAGCTCGGt                                                                 <  1:675379/140‑1 (MQ=255)                                                                 ccGTCACCGATTTTGCCGGTTTGCGCCGTGCGAATAATGGTATCGACACAGGTATCGACAATGTCGTCAGGTACGACAATCTCAATTTTCACTTTCGGCAGAAAATCCACCATATACTCCGCGCCGCGGTACAGCTCGGt                                                                 <  1:509988/140‑1 (MQ=255)                                                                     cACCGATTTTGCCGGTTTGCGCCGTGCGAATAATGGTATCGACACAGGTATCGACAATGTCGTCAGGTACGACAATCTCAATTTTCACTTTCGGCAGAAAATCCACCATATACTCCGCGCCGCGGTACAGCTCGGTATgg                                                             >  1:700119/1‑140 (MQ=255)                                                                     cACCGATTTTGCCGGTTTGCGCCGTGCGAATAATGGTATCGACACAGGTATCGACAATGTCGTCAGGTACGACAATCTCAATTTTCACTTTCGGCAGAAAATCCACCATATACTCCGCGCCGCGGTACAGCTCGGTATgg                                                             >  2:599014/1‑140 (MQ=255)                                                                     cACCGATTTTGCCGGTTTGCGCCGTGCGAATAATGGTATCGACACAGGTATCGACAATGTCGTCAGGTACGACAATCTCAATTTTCACTTTCGGCAGAAAATCCACCATATACTCCGCGCCGCGGTACAGCTCGGTATgg                                                             >  2:694702/1‑140 (MQ=255)                                                                              tGCCGGTTTGCGCCGTGCGAATAATGGTATCGACACAGGTATCGACAATGTCGTCAGGTACGACAATCTCAATTTTCACTTTCGGCAGAAAATCCACCATATACTCCGCGCCGCGGTACAGCTCGGTATGGCCTTTCTgg                                                    >  1:478150/1‑140 (MQ=255)                                                                              tGCCGGTTTGCGCCGTGCGAATAATGGTATCGACACAGGTATCGACAATGTCGTCAGGTACGACAATCTCAATTTTCACTTTCGGCAGAAAATCCACCATATACTCCGCGCCGCGGTACAGCTCGGTATGGCCTTTCTgg                                                    >  1:218757/1‑140 (MQ=255)                                                                                  ggTTTGCGCCGTGCGAATAATGGTATCGACACAGGTATCGACAATGTCGTCAGGTACGACAATCTCAATTTTCACTTTCGGCAGAAAATCCACCATATACTCCGCGCCGCGGTACAGCTCGGTATGGCCTTTCTGGCGAc                                                <  2:518541/140‑1 (MQ=255)                                                                                     ttGCGCCGTGCGAATAATGGTATCGACACAGGTATCGACAATGTCGTCAGGTACGACAATCTCAATTTTCACTTTCGGCAGAAAATCCACCATATACTCCGCGCCGCGGTACAGCTCGGTATGGCCTTTCTGGCGACCaa                                             <  2:510377/140‑1 (MQ=255)                                                                                       gcgcCGTGCGAATAATGGTATCGACACAGGTATCGACAATGTCGTCAGGTACGACAATCTCAATTTTCACTTTCGGCAGAAAATCCACCATATACTCCGCGCCGCGGTACAGCTCGGTATGGCCTTTCTGGCGACCAAAg                                           <  1:236924/140‑1 (MQ=255)                                                                                          ccGTGCGAATAATGGTATCGACACAGGTATCGACAATGTCGTCAGGTACGACAATCTCAATTTTCACTTTCGGCAGAAAATCCACCATATACTCCGCGCCGCGGTACAGCTCGGTATGGCCTTTCTGGCGACCAAAGCCt                                        >  2:302085/1‑140 (MQ=255)                                                                                                gAATAATGGTATCGACACAGGTATCGACAATGTCGTCAGGTACGACAATCTCAATTTTCACTTTCGGCAGAAAATCCACCATATACTCCGCGCCGCGGTACAGCTCGGTATGGCCTTTCTGGCGACCAAAGCCTTTCACt                                  <  1:344094/140‑1 (MQ=255)                                                                                                     atGGTATCGACACAGGTATCGACAATGTCGTCAGGTACGACAATCTCAATTTTCACTTTCGGCAGAAAATCCACCATATACTCCGCGCCGCGGTACAGCTCGGTATGGCCTTTCTGGCGACCAAAGCCTTTCACTTCGGt                             <  1:694702/140‑1 (MQ=255)                                                                                                              acacAGGTATCGACAATGTCGTCAGGTACGACAATCTCAATTTTCACTTTCGGCAGAAAATCCACCATATACTCCGCGCCGCGGTACAGCTCGGTATGGCCTTTCTGGCGACCAAAGCCTTTCACTTCGGTCACCGTCAt                    <  2:480170/140‑1 (MQ=255)                                                                                                              acacAGGTATCGACAATGTCGTCAGGTACGACAATCTCAATTTTCACTTTCGGCAGAAAATCCACCATATACTCCGCGCCGCGGTACAGCTCGGTATGGCCTTTCTGGCGACCAAAGCCTTTCACTTCGGTCACCGTCAt                    >  2:289761/1‑140 (MQ=255)                                                                                                                acaGGTATCGACAATGTCGTCAGGTACGACAATCTCAATTTTCACTTTCGGCAGAAAATCCACCATATACTCCGCGCCGCGGTACAGCTCGGTATGGCCTTTCTGGCGACCAAAGCCTTTCACTTCGGTCACCGTCATGc                  >  1:15444/1‑140 (MQ=255)                                                                                                                  aGGTATCGACAATGTCGTCAGGTACGACAATCTCAATTTTCACTTTCGGCAGAAAATCCACCATATACTCCGCGCCGCGGTACAGCTCGGTATGGCCTTTCTGGCGACCAAAGCCTTTCACTTCGGTCACCGTCATGCCg                <  2:230856/140‑1 (MQ=255)                                                                                                                      aTCGACAATGTCGTCAGGTACGACAATCTCAATTTTCACTTTCGGCAGAAAATCCACCATATACTCCGCGCCGCGGTACAGCTCGGTATGGCCTTTCTGGCGACCAAAGCCTTTCACTTCGGTCACCGTCATGCCGGTaa            >  2:361089/1‑140 (MQ=255)                                                                                                                          aCAATGTCGTCAGGTACGACAATCTCAATTTTCACTTTCGGCAGAAAATCCACCATATACTCCGCGCCGCGGTACAGCTCGGTATGGCC‑TTCTGGCGACCAAAGCCTTTCACTTCGGTCACCGTCATGCCGGTAATACCg       >  2:129066/1‑140 (MQ=255)                                                                                                                             aTGTCGTCAGGTACGACAATCTCAATTTTCACTTTCGGCAGAAAATCCACCATATACTCCGCGCCGCGGTACAGCTCGGTATGGCCTTTCTGGCGACCAAAGCCTTTCACTTCGGTCACCGTCATGCCGGTAATACCGAc     <  1:599014/140‑1 (MQ=255)                                                                                                                              tGTCGTCAGGTACGACAATCTCAATTTTCACTTTCGGCAGAAAATCCACCATATACTCCGCGCCGCGGTACAGCTCGGTATGGCCTTTCTGGCGACCAAAGCCTTTCTCTTCGGTCACCGTCATGCCGGTAATACCGACt    >  1:368442/1‑140 (MQ=255)                                                                                                                              tGTCGTCAGGTACGACAATCTCAATTTTCACTTTCGGCAGAAAATCCACCATATACTCCGCGCCGCGGTACAGCTCGGTATGGCCTTTCTGGCGACCAAAGCCTTTCACTTCGGTCACCGTCATGCCGGTAATACCGACt    >  2:10703/1‑140 (MQ=255)                                                                                                                              tGTCGTCAGGTACGACAATCTCAATTTTCACTTTCGGCAGAAAATCCACCATATACTCCGCGCCGCGGTACAGCTCGGTATGGCCTTTCTGGCGACCAAAGCCTTTCACTTCGGTCACCGTCATGCCGGTAATACCGACt    >  1:699328/1‑140 (MQ=255)                                                                                                                              tGTCGTCAGGTACGACAATCTCAATTTTCACTTTCGGCAGAAAATCCACCATATACTCCGCGCCGCGGTACAGCTCGGTATGGCCTTTCTGGCGACCAAAGCCTTTCACTTCGGTCACCGTCATGCCGGTAATACCGACt    <  1:432240/140‑1 (MQ=255)                                                                                                                              tGTCGTCAGGTACGACAATCTCAATTTTCACTTTCGGCAGAAAATCCACCATATACTCCGCGCCGCGGTACAGCTCGGTATGGCCTTTCTGGCGACCAAAGCCTTTCACTTCGGTCACCGTCATGCCGGTAATACCGACt    >  1:276762/1‑140 (MQ=255)                                                                                                                              tGTCGTCAGGTACGACAATCTCAATTTTCACTTTCGGCAGAAAATCCACCATATACTCCGCGCCGCGGTACAGCTCGGTATGGCCTTTCTGGCGACCAAAGCCTTTCACTTCGGTCACCGTCATGCCGGTAATACCGACt    >  1:206023/1‑140 (MQ=255)                                                                                                                                tcgtcAGGTACGACAATCTCAATTTTCACTTTCGGCAGAAAATCCACCATATACTCCGCGCCGCGGTACAGCTCGGTATGGCCTTTCTGGCGACCAAAGCCTTTCTCTTCGGTCACCGTCATGCCGGTAATACCGACTTc  <  2:368442/140‑1 (MQ=255)                                                                                                                                tcgtcAGGTACGACAATCTCAATTTTCACTTTCGGCAGAAAATCCACCATATACTCCGCGCCGCGGTACAGCTCGGTATGGCCTTTCTGGCGACCAAAGCCTTTCACTTCGGTCACCGTCATGCCGGTAATACCGACTTc  <  2:478150/140‑1 (MQ=255)                                                                                                                                     |                                                                                                                                       CGCGTCGTCCTCTTCACCGGTACGGATGCGAATGACCCGTGCCACGTCAAAGACGAAGATTTTACCGTCACCGATTTTGCCGGTTTGCGCCGTGCGAATAATGGTATCGACACAGGTATCGACAATGTCGTCCGGTACGACAATCTCAATTTTCACTTTCGGCAGAAAATCCACCATATACTCCGCGCCGCGGTACAGCTCGGTATGGCCTTTCTGGCGACCAAAGCCTTTCACTTCGGTCACCGTCATGCCGGTAATACCGACTTC  >  NC_000913/2687079‑2687345

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑