Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,961,169	C→A	P420P (CCG→CCT)	recC ←	exodeoxyribonuclease V subunit RecC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,961,169	0	C	A	100.0%	172.3 / NA	50	P420P (CCG→CCT)	recC	exodeoxyribonuclease V subunit RecC
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (28/22);  total (28/22)

AAAACGACTGTCAGGCAGTGATAACAGGCTGATAAACGCTTCCAGTACCGGATGTGACTGCCGCGCACGACGGTCGGAAATGGCGTAAGGTAGGTAACGATCCGCAGGTGCGCTACCAAACACAGCCTGAATAAACGGACTGTAGCTGTCGATATCAGCCACCATCACGATGATGTCGCGCGGAGTAAGTGTCGGGTCTTCCTCCAGCATCGCCAGCAGGCGATCGTGTAAAACTTCAACTTCACGCTGCGGGCTATGGCAAACGTGGA  >  NC_000913/2961034‑2961302                                                                                                                                        |                                                                                                                                      aaaaCGACTGTCAGGCAGTGATAACAGGCTGATAAACGCTTCCAGTACCGGATGTGACTGCCGCGCACGACGGTCGGAAATGGCGTAAGGTAGGTAACGATCCGCAGGTGCGCTACCAAACACAGCCTGAATAAAAGGAc                                                                                                                                   >  2:315834/1‑140 (MQ=255)   aaCGACTGTCAGGCAGTGATAACAGGCTGATAAACGCTTCCAGTACCGGATGTGACTGCCGCGCACGACGGTCGGAAATGGCGTAAGGTAGGTAACGATCCGCAGGTGCGCTACCAAACACAGCCTGAATAAAAGGACTg                                                                                                                                 >  1:696217/1‑140 (MQ=255)      gACTGTCAGGCAGTGATAACAGGCTGATAAACGCTTCCAGTACCGGATGTGACTGCCGCGCACGACGGTCGGAAATGGCGTAAGGTAGGTAACGATCCGCAGGTGCGCTACCAAACACAGCCTGAATAAAAGGACTGTAg                                                                                                                              <  2:682244/140‑1 (MQ=255)       aCTGTCAGGCAGTGATAACAGGCTGATAAACGCTTCCAGTACCGGATGTGACTGCCGCGCACGACGGTCGGAAATGGCGTAAGGTAGGTAACGATCCGCAGGTGCGCTACCAAACACAGCCTGAATAAAAGGACTGTAGc                                                                                                                             >  1:689955/1‑140 (MQ=255)                  gTGATAACAGGCTGATAAACGCTTCCAGTACCGGATGTGACTGCCGCGCACGACGGTCGGAAATGGCGTAAGGTAGGTAACGATCCGCAGGTGCGCTACCAAACACAGCCTGAATAAAAGGACTGTAGCTGTCGATATCa                                                                                                                  >  1:661801/1‑140 (MQ=255)                    gATAACAGGCTGATAAACGCTTCCAGTACCGGATGTGACTGCCGCGCACGACGGTCGGAAATGGCGTAAGGTAGGTAACGATCCGCAGGTGCGCTACCAAACACAGCCTGAATAAAAGGACTGTAGCTGTCGATATCAGc                                                                                                                <  2:689955/140‑1 (MQ=255)                     aTAACAGGCTGATAAACGCTTCCAGTACCGGATGTGACTGCCGCGCACGACGGTCGGAAATGGCGTAAGGTAGGTAACGATCCGCAGGTGCGCTACCAAACACAGCCTGAATAAAAGGACTGTAGCTGTCGATATCAGcc                                                                                                               >  2:198938/1‑140 (MQ=255)                     aTAACAGGCTGATAAACGCTTCCAGTACCGGATGTGACTGCCGCGCACGACGGTCGGAAATGGCGTAAGGTAGGTAACGATCCGCAGGTGCGCTACCAAACACAGCCTGAATAAAAGGACTGTAGCTGTCGATATCAGcc                                                                                                               >  1:16455/1‑140 (MQ=255)                       aaCAGGCTGATAAACGCTTCCAGTACCGGATGTGACTGCCGCGCACGACGGTCGGAAATGGCGTAAGGTAGGTAACGATCCGCAGGTGCGCTACCAAACACAGCCTGAATAAAAGGACTGTAGCTGTCGATATCAGccac                                                                                                             <  2:661801/140‑1 (MQ=255)                          aGGCTGATAAACGCTTCCAGTACCGGATGTGACTGCCGCGCACGACGGTCGGAAATGGCGTAAGGTAGGTAACGATCCGCAGGTGCGCTACCAAACACAGCCTGAATAAAAGGACTGTAGCTGTCGATATCAGCCACCAt                                                                                                          <  1:198938/140‑1 (MQ=255)                             cTGATAAACGCTTCCAGTACCGGATGTGACTGCCGCGCACGACGGTCGGAAATGGCGTAAGGTAGGTAACGATCCGCAGGTGCGCTACCAAACACAGCCTGAATAAAAGGACTGTAGCTGTCGATATCAGc                                                                                                                <  2:801630/131‑1 (MQ=255)                             cTGATAAACGCTTCCAGTACCGGATGTGACTGCCGCGCACGACGGTCGGAAATGGCGTAAGGTAGGTAACGATCCGCAGGTGCGCTACCAAACACAGCCTGAATAAAAGGACTGTAGCTGTCGATATCAGc                                                                                                                >  1:801630/1‑131 (MQ=255)                               gATAAACGCTTCCAGTACCGGATGTGACTGCCGCGCACGACGGTCGGAAATGGCGTAAGGTAGGTAACGATCCGCAGGTGCGCTACCAAACACAGCCTGAATAAAAGGACTGTAGCTGTCGATATCAGCCACCATCACga                                                                                                     >  2:669902/1‑140 (MQ=255)                                aTAAACGCTTCCAGTACCGGATGTGACTGCCGCGCACGACGGTCGGAAATGGCGTAAGGTAGGTAACGATCCGCAGGTGCGCTACCAAACACAGCCTGAATAAAAGGACTGTAGCTGTCGATATCAGCCACCATCACgat                                                                                                    >  1:343693/1‑140 (MQ=255)                                          ccAGTACCGGATGTGACTGCCGCGCACGACGGTCGGAAATGGCGTAAGGTAGGTAACGATCCGCAGGTGCGCTACCAAACACAGCCTGAATAAAAGGACTGTAGCTGTCGATATCAGCCACCATCACGATGATGTcgcgc                                                                                          >  1:267330/1‑140 (MQ=255)                                              tACCGGATGTGACTGCCGCGCACGACGGTCGGAAATGGCGTAAGGTAGGTAACGATCCGCAGGTGCGCTACCAAACACAGCCTGAATAAAAGGACTGTAGCTGTCGATATCAGCCACCATCACGATGATGTCGCGCGGAg                                                                                      <  2:72040/140‑1 (MQ=255)                                                   gATGTGACTGCCGCGCACGACGGTCGGAAATGGCGTAAGGTAGGTAACGATCCGCAGGTGCGCTACCAAACACAGCCTGAATAAAAGGACTGTAGCTGTCGATATCAGCCACCATCACGATGATGTCGCGCGGAGTAAgt                                                                                 >  1:448081/1‑140 (MQ=255)                                                      gtgACTGCCGCGCACGACGGTCGGAAATGGCGTAAGGTAGGTAACGATCCGCAGGTGCGCTACCAAACACAGCCTGAATAAAAGGACTGTAGCTGTCGATATCAGCCACCATCACGATGATGTCGCGCGGAGTAAGTGTc                                                                              >  2:550903/1‑140 (MQ=255)                                                          cTGCCGCGCACGACGGTCGGAAATGGCGTAAGGTAGGTAACGATCCGCAGGTGCGCTACCAAACACAGCCTGAATAAAAGGACTGTAGCTGTCGATATCAGCCACCATCACGTTGATGTCGCGCGGAGTAAGTGTCGGGt                                                                          <  1:133283/140‑1 (MQ=255)                                                          cTGCCGCGCACGACGGTCGGAAATGGCGTAAGGTAGGTAACGATCCGCAGGTGCGCTACCAAACACAGCCTGAATAAAAGGACTGTAGCTGTCGATATCAGCCACCATCACGATGATGTCGCGCGGAGTAAGTGTCGGGt                                                                          <  1:669902/140‑1 (MQ=255)                                                          cTGCCGCGCACGACGGTCGGAAATGGCGTAAGGTAGGTAACGATCCGCAGGTGCGCTACCAAACACAGCCTGAATAAAAGGACTGTAGCTGTCGATATCAGCCACCATCACGATGATGTCGCGCGGAGTAAGTGTCGGGt                                                                          <  2:696217/140‑1 (MQ=255)                                                                   acgacgGTCGGAAATGGCGTAAGGTAGGTAACGATCCGCAGGTGCGCTACCAAACACAGCCTGAATAAAAGGACTGTAGCTGTCGATATCAGCCACCATCACGATGATGTCGCGCGGAGTAAGTGTCGGGTCTTCCTCCa                                                                 >  2:255623/1‑140 (MQ=255)                                                                        ggTCGGAAATGGCGTAAGGTAGGTAACGATCCGCAGGTGCGCTACCAAACACAGCCTGAATAAAAGGACTGTAGCTGTCGATATCAGCCACCATCACGATGATGTCGCGCGGAGTAAGTGTCGGGTCTTCCTCCAGCATc                                                            <  1:555561/140‑1 (MQ=255)                                                                          tCGGAAATGGCGTAAGGTAGGTAACGATCCGCAGGTGCGCTACCAAACACAGCCTGAATAAAAGGACTGTAGCTGTCGATATCAGCCACCATCACGATGATGTCGCGCGGAGTAAGTGTCGGGTCTTCCTCCAGCa                                                              >  1:694838/1‑136 (MQ=255)                                                                          tCGGAAATGGCGTAAGGTAGGTAACGATCCGCAGGTGCGCTACCAAACACAGCCTGAATAAAAGGACTGTAGCTGTCGATATCAGCCACCATCACGATGATGTCGCGCGGAGTAAGTGTCGGGTCTTCCTCCAGCa                                                              <  2:694838/136‑1 (MQ=255)                                                                          tCGGAAATGGCGTAAGGTAGGTAACGATCCGCAGGTGCGCTACCAAACACAGCCTGAATAAAAGGACTGTAGCTGTCGATATCAGCCACCATCACGATGATGTCGCGCGGAGTAAGTGTCGGGTCTTCCTCCAGCATCGc                                                          <  2:98559/140‑1 (MQ=255)                                                                          tCGGAAATGGCGTAAGGTAGGTAACGATCCGCAGGTGCGCTACCAAACACAGCCTGAATAAAAGGACTGTAGCTGTCGATATCAGCCACCATCACGATGATGTCGCGCGGAGTAAGTGTCGGGTCTTCCTCCAGCATCGc                                                          <  2:117559/140‑1 (MQ=255)                                                                               aaTGGCGTAAGGTAGGTAACGATCCGCAGGTGCGCTACCAAACACAGCCTGAATAAAAGGACTGTAGCTGTCGATATCAGCCACCATCACGATGATGTCGCGCGGAGTAAGTGTCGGGTCTTCCTCCAGCATCGCcagca                                                     >  2:768998/1‑140 (MQ=255)                                                                                 tGGCGTAAGGTAGGTAACGATCCGCAGGTGCGCTACCAAACACAGCCTGAATAAAAGGACTGTAGCTGTCGATATCAGCCACCATCACGATGATGTCGCGCGGAGTAAGTGTCGGGTCTTCCTCCAGCATCGCCAGCAgg                                                   <  2:667913/140‑1 (MQ=255)                                                                                        aggtaggtAACGATCCGCAGGTGCGCTACCAAACACAGCCTGAATAAAAGGACTGTAGCTGTCGATATCAGCCACCATCACGATGATGTCGCGCGGAGTAAGTGTCGGGTCTTCCTCCAGCATCGCCAGCAGGCGATCgt                                            >  1:123117/1‑140 (MQ=255)                                                                                                   gATCCGCAGGTGCGCTACCAAACACAGCCTGAATAAAAGGACTGTAGCTGTCGATATCAGCCACCATCACGATGATGTCGCGCGGAGTAAGTGTCGGGTCTTCCTCCAGCATCGCCAGCAGGCGATCGTGTAAaacttca                                 >  1:164563/1‑140 (MQ=255)                                                                                                     tCCGCAGGTGCGCTACCAAACACAGCCTGAATAAAAGGACTGTAGCTGTCGATATCAGCCACCATCACGATGATGTCGCGCGGAGTAAGTGTCGGGTCTTCCTCCAGCATCGCCAGCAGGCGATCGTGTAAaacttcaac                               >  2:588099/1‑140 (MQ=255)                                                                                                        gCAGGTGCGCTACCAAACACAGCCTGAATAAAAGGACTGTAGCTGTCGATATCAGCCACCATCACGATGATGTCGCGCGGAGTAAGTGTCGGGTCTTCCTCCAGCATCGCCAGCAGGCGATCGTGTAAaacttcaacttc                            >  2:111801/1‑140 (MQ=255)                                                                                                             tGCGCTACCAAACACAGCCTGAATAAAAGGACTGTAGCTGTCGATATCAGCCACCATCACGATGATGTCGCGCGGAGTAAGTGTCGGGTCTTCCTCCAGCATCGCCAGCAGGCGATCGTGTAAAACTTCAACTTCACGCt                       <  1:588099/140‑1 (MQ=255)                                                                                                               cgcTACCAAACACAGCCTGAATAAAAGGACTGTAGCTGTCGATATCAGCCACCATCACGATGATGTCGCGCGGAGTAAGTGTCGGGTCTTCCTCCAGCATCGCCAGCAGGCGATCGTGTAAAACTTCAACTTCACGCTGc                     >  1:413156/1‑140 (MQ=255)                                                                                                               cgcTACCAAACACAGCCTGAATAAAAGGACTGTAGCTGTCGATATCAGCCACCATCACGATGATGTCGCGCGGAGTAAGTGTCGGGTCTTCCTCCAGCATCGCCAGCAGGCGATCGTGTAAAACTTCAACTTCACGCTGc                     >  1:401936/1‑140 (MQ=255)                                                                                                                  tACCAAACACAGCCTGAATAAAAGGACTGTAGCTGTCGATATCAGCCACCATCACGATGATGTCGCGCGGAGTAAGTGTCGGGTCTTCCTCCAGCATCGCCAGCAGGCGATCGTGTAAAACTTCAACTTCACGCTGCggg                  <  2:164563/140‑1 (MQ=255)                                                                                                                              ccTGAATAAAAGGACTGTAGCTGTCGATATCAGCCACCATCACGATGATGTCGCGCGGAGTAAGTGTCGGGTCTTCCTCCAGCATCGCCAGCAGGCGATCGTGTAAAACTTCAACTTCACGCTg                      <  1:239074/124‑1 (MQ=255)                                                                                                                              ccTGAATAAAAGGACTGTAGCTGTCGATATCAGCCACCATCACGATGATGTCGCGCGGAGTAAGTGTCGGGTCTTCCTCCAGCATCGCCAGCAGGCGATCGTGTAAAACTTCAACTTCACGCTg                      >  2:239074/1‑124 (MQ=255)                                                                                                                              ccTGAATAAAAGGACTGTAGCTGTCGATATCAGCCACCATCACGATGATGTCGCGCGGAGTAAGTGTCGGGTCTTCCTCCAGCATCGCCAGCAGGCGATCGTGTAAAACTTCAACTTCACGCTGCg                    >  2:481215/1‑126 (MQ=255)                                                                                                                              ccTGAATAAAAGGACTGTAGCTGTCGATATCAGCCACCATCACGATGATGTCGCGCGGAGTAAGTGTCGGGTCTTCCTCCAGCATCGCCAGCAGGCGATCGTGTAAAACTTCAACTTCACGCTGCg                    <  1:481215/126‑1 (MQ=255)                                                                                                                              ccTGAATAAAAGGACTGTAGCTGTCGATATCAGCCACCATCACGATGATGTCGCGCGGAGTAAGTGTCGGGTCTTCCTCCAGCATCGCCAGCAGGCGATCGTGTAAAACTTCAACTTCACGCTGCGGGCTATGGCa          >  1:815066/1‑136 (MQ=255)                                                                                                                              ccTGAATAAAAGGACTGTAGCTGTCGATATCAGCCACCATCACGATGATGTCGCGCGGAGTAAGTGTCGGGTCTTCCTCCAGCATCGCCAGCAGGCGATCGTGTAAAACTTCAACTTCACGCTGCGGGCTATGGCa          <  2:815066/136‑1 (MQ=255)                                                                                                                              ccTGAATAAAAGGACTGTAGCTGTCGATATCAGCCACCATCACGATGATGTCGCGCGGAGTAAGTGTCGGGTCTTCCTCCAGCATCGCCAGCAGGCGATCGTGTAAAACTTCAACTTCACGCTGCGGGCTATGGCAAACg      >  2:758055/1‑140 (MQ=255)                                                                                                                              ccTGAATAAAAGGACTGTAGCTGTCGATATCAGCCACCATCACGATGATGTCGCGCGGAGTAAGTGTCGGGTCTTCCTCCAGCATCGCCAGCAGGCGATCGTGTAAAACTTCAACTTCACGCTGCGGGCTATGGCAAACg      >  2:760077/1‑140 (MQ=255)                                                                                                                              ccTGAATAAAAGGACTGTAGCTGTCGATATCAGCCACCATCACGATGATGTCGCGCGGAGTAAGTGTCGGGTCTTCCTCCAGCATCGCCAGCAGGCGATCGTGTAAAACTTCAACTTCACGCTGCGGGCTATGGCAAACg      <  1:111801/140‑1 (MQ=255)                                                                                                                                tGAATAAAAGGACTGTAGCTGTCGATATCAGCCACCATCACGATGATGTCGCGCGGAGTAAGTGTCGGGTCTTCCTCCAGCATCGCCAGCAGGCGATCGTGTAAAACTTCAACTTCACGCTGCGGGCTATGGCAAACGTg    <  2:448081/140‑1 (MQ=255)                                                                                                                                  aaTAAAAGGACTGTAGCTGTCGATATCAGCCACCATCACGATGATGTCGCGCGGAGTAAGTGTCGGGTCTTCCTCCAGCATCGCCAGCAGGCGATCGTGTAAAACTTCAACTTCACGCTGCGGGCTATGGCAAACGTGGa  >  1:90287/1‑140 (MQ=255)                                                                                                                                  aaTAAAAGGACTGTAGCTGTCGATATCAGCCACCATCACGATGATGTCGCGCGGAGTAAGTGTCGGGTCTTCCTCCAGCATCGCCAGCAGGCGATCGTGTAAAACTTCAACTTCACGCTGCGGGCTATGGCAAACGTGGa  <  2:166984/140‑1 (MQ=255)                                                                                                                                  aaTAAAAGGACTGTAGCTGTCGATATCAGCCACCATCACGATGATGTCGCGCGGAGTAAGTGTCGGGTCTTCCTCCAGCATCGCCAGCAGGCGATCGTGTAAAACTTCAACTTCACGCTGCGGGCTATGGCAAACGTGGa  >  1:166984/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                      AAAACGACTGTCAGGCAGTGATAACAGGCTGATAAACGCTTCCAGTACCGGATGTGACTGCCGCGCACGACGGTCGGAAATGGCGTAAGGTAGGTAACGATCCGCAGGTGCGCTACCAAACACAGCCTGAATAAACGGACTGTAGCTGTCGATATCAGCCACCATCACGATGATGTCGCGCGGAGTAAGTGTCGGGTCTTCCTCCAGCATCGCCAGCAGGCGATCGTGTAAAACTTCAACTTCACGCTGCGGGCTATGGCAAACGTGGA  >  NC_000913/2961034‑2961302

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑