Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	717,225	A→G	S347S (AGT→AGC)	potE ←	putrescine:H(+) symporter/putrescine:ornithine antiporter PotE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	717,225	0	A	G	100.0%	196.0 / NA	53	S347S (AGT→AGC)	potE	putrescine:H(+) symporter/putrescine:ornithine antiporter PotE
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (23/30);  total (23/30)

AAGTTTGCAACTTTCGCTTTTGAGGGGGGCACATTAGCGACCTTCTGAATAATGACTAATGCCGCCATTGACAGAATATACGGAATGATATTGGTCACCACGGCCAGGTTAACCAGCACGTTGAACTGACTGTTTAGCGACGGGCTAATGGTCATCAGTGCCAATCCACTCTGAATAATCACAATGGTCAACATTCCCTGCACCGGTGCATCCACTTTGGTTACACGGGAGAAAATTTTAGGGAAGTAGCCTTCATCAGATGAAG  >  NC_000913/717097‑717361                                                                                                                                 |                                                                                                                                         aaGTTTGCAACTTTCGCTTTTGAGGGGGGCACATTAGCGACCTTCTGAATAATGACTAATGCCGCCATTGACAGAATATACGGAATGATATTGGTCACCACGGCCAGGTTAACCAGCACGTTGAACTGGCTGTTTAGCGa                                                                                                                               <  1:194136/140‑1 (MQ=255) aaGTTTGCAACTTTCGCTTTTGAGGGGGGCACATTAGCGACCTTCTGAATAATGACTAATGCCGCCATTGACAGAATATACGGAATGATATTGGTCACCACGGCCAGGTTAACCAGCACGTTGAACTGGCTGTTTAGCGa                                                                                                                               <  2:815434/140‑1 (MQ=255) aaGTTTGCAACTTTCGCTTTTGAGGGGGGCACATTAGCGACCTTCTGAATAATGACTAATGCCGCCATTGACAGAATATACGGAATGATATTGGTCACCACGGCCAGGTTAACCAGCACGTTGAACTGGCTGTTTAGCGa                                                                                                                               <  2:280314/140‑1 (MQ=255) aaGTTTGCAACTTTCGCTTTTGAGGGGGGCACATTAGCGACCTTCTGAATAATGACTAATGCCGCCATTGACAGAATATACGGAATGATATTGGTCACCACGGCCAGGTTAACCAGCACGTTGAACTGGCTGTTTAGCGa                                                                                                                               <  1:839960/140‑1 (MQ=255) aaGTTTGCAACTTTCGCTTTTGAGGGGGGCACATTAGCGACCTTCTGAATAATGACTAATGCCGCCATTGACAGAATATACGGAATGATATTGGTCACCACGGCCAGGTTAACCAGCACGTTGAACTGGCTGTTTAGCGa                                                                                                                               <  1:704924/140‑1 (MQ=255)      tGCAACTTTCGCTTTTGAGGGGGGCACATTAGCGACCTTCTGAATAATGACTAATGCCGCCATTGACAGAATATACGGAATGATATTGGTCACCACGGCCAGGTTAACCAGCACGTTGAACTGGCTGTTTAGCGACGGGc                                                                                                                          <  1:627236/140‑1 (MQ=255)       gCAACTTTCGCTTTTGAGGGGGGCACATTAGCGACCTTCTGAATAATGACTAATGCCGCCATTGACAGAATATACGGAATGATATTGGTCACCACGGCCAGGTTAACCAGCACGTTGAACTGGCTGTTTAGCGACGGGCt                                                                                                                         >  1:389563/1‑140 (MQ=255)        cAACTTTCGCTTTTGAGGGGGGCACATTAGCGACCTTCTGAATAATGACTAATGCCGCCATTGACAGAATATACGGAATGATATTGGTCACCACGGCCAGGTTAACCAGCACGTTGAACTGGCTGTTTAGCGACGGGCTa                                                                                                                        <  2:862409/140‑1 (MQ=255)            tttCGCTTTTGAGGGGGGCACATTAGCGACCTTCTGAATAATGACTAATGCCGCCATTGACAGAATATACGGAATGATATTGGTCACCACGGCCAGGTTAGCCAGCACGTTGAACTGGCTGTTTAGCGACGGGCTAATgg                                                                                                                    <  2:278877/140‑1 (MQ=255)                      gAGGGGGGCACATTAGCGACCTTCTGAATAATGACTAATGCCGCCATTGACAGAATATACGGAATGATATTGGTCACCACGGCCAGGTTAACCAGCACGTTGAACTGGCTGTTTAGCGACGGGCTAATGGTCATCAGTGc                                                                                                          <  2:371975/140‑1 (MQ=255)                           gggCACATTAGCGACCTTCTGAATAATGACTAATGCCGCCATTGACAGAATATACGGAATGATATTGGTCACCACGGCCAGGTTAACCAGCACGTTGAACTGGCTGTTTAGCGACGGGCTAATGGTCATCAGTGCCAATc                                                                                                     <  1:394502/140‑1 (MQ=255)                            ggCACATTAGCGACCTTCTGAATAATGACTAATGCCGCCATTGACAGAATATACGGAATGATATTGGTCACCACGGCCAGGTTAACCAGCACGTTGAACTGGCTGTTTAGCGAC‑GGCTAATGGTCATCAGTGCCAATCCa                                                                                                   <  2:418576/140‑1 (MQ=255)                              cacaTTAGCGACCTTCTGAATAATGACTAATGCCGCCATTGACAGAATATACGGAATGATATTGGTCACCACGGCCAGGTTAACCAGCACGTTGAACTGGCTGTTTAGCGACGGGCTAATGGTCATCAGTGCCAATCCAc                                                                                                  >  2:101245/1‑140 (MQ=255)                                   tAGCGACCTTCTGAATAATGACTAATGCCGCCATTGACAGAATATACGGAATGATATTGGTCACCACGGCCAGGTTAACCAGCACGTTGAACTGGCTGTTTAGCGACGGGCTAATGGTCATCAGTGCCAATCCACTCTGa                                                                                             <  1:290295/140‑1 (MQ=255)                                         ccTTCTGAATAATGACTAATGCCGCCATTGACAGAATATACGGAATGATATTGGTCACCACGGCCAGGTTAACCAGCACGTTGAACTGGCTGTTTAGCGACGGGCTAATGGTCATCAGTGCCAATCCACTCTGAATAATc                                                                                       <  1:101245/140‑1 (MQ=255)                                          cTTCTGAATAATGACTAATGCCGCCATTGACAGAATATACGGAATGATATTGGTCACCACGGCCAGGTTAACCAGCACGTTGAACTGGCTGTTTAGCGACGGGCTAATGGTCATCAGTGCCAATCCACTCTGAATAATca                                                                                      <  1:757680/140‑1 (MQ=255)                                           ttCTGAATAATGACTAATGCCGCCATTGACAGAATATACGGAATGATATTGGTCACCACGGCCAGGTTAACCAGCACGTTGAACTGGCTGTTTAGCGACGGGCTAATGGTCATCAGTGCCAATCCActc                                                                                                <  1:836690/129‑1 (MQ=255)                                           ttCTGAATAATGACTAATGCCGCCATTGACAGAATATACGGAATGATATTGGTCACCACGGCCAGGTTAACCAGCACGTTGAACTGGCTGTTTAGCGACGGGCTAATGGTCATCAGTGCCAATCCActc                                                                                                >  2:836690/1‑129 (MQ=255)                                           ttCTGAATAATGACTAATGCCGCCATTGACAGAATATACGGAATGATATTGGTCACCACGGCCAGGTTAACCAGCACGTTGAACTGGCTGTTTAGCGACGGGCTAATGGTCATCAGTGCCAATCCACTCTGAATAATcac                                                                                     >  2:428908/1‑140 (MQ=255)                                           ttCTGAATAATGACTAATGCCGCCATTGACAGAATATACGGAATGATATTGGTCACCACGGCCAGGTTAACCAGCACGTTGAACTGGCTGTTTAGCGACGGGCTAATGGTCATCAGTGCCAATCCACTCTGAATAATcac                                                                                     >  2:630563/1‑140 (MQ=255)                                           ttCTGAATAATGACTAATGCCGCCATTGACAGAATATACGGAATGATATTGGTCACCACGGCCAGGTTAACCAGCACGTTGAACTAGCTGTTTAGCGACGGGCTAATGGTCATCAGTGCCAATCCACTCTGAATAATcac                                                                                     >  2:237450/1‑140 (MQ=255)                                            tCTGAATAATGACTAATGCCGCCATTGACAGAATATACGGAATGATATTGGTCACCACGGCCAGGTTAACCAGCACGTTGAACTGGCTGTTTAGCGACGGGCTAATGGTCATCAGTGCCAATCCACTCTGAATAATcaca                                                                                    <  2:528541/140‑1 (MQ=255)                                            tCTGAATAATGACTAATGCCGCCATTGACAGAATATACGGAATGATATTGGTCACCACGGCCAGGTTAACCAGCACGTTGAACTGGCTGTTTAGCGACGGGCTAATGGTCATCAGTGCCAATCCACTCTGAATAATcaca                                                                                    <  1:428908/140‑1 (MQ=255)                                            tCTGAATAATGACTAATGCCGCCATTGACAGAATATACGGAATAATATTGGTCCGCACGGCCAGGTTAACCAGCACGTTGAACTGGCTGTTTAGCGACGGGATAATGGTCATCAGTGCCAATCCACTCTGAAGAATcaca                                                                                    <  2:43737/140‑1 (MQ=255)                                             cTGAATAATGACTAATGCCGCCATTGACAGAATATACGGAATGATATTGGTCACCACGGCCAGGTTAACCAGCACGTTGAACTGGCTGTTTAGCGACGGGCTAATGGTCATCAGTGCCAATCCACTCTGAATAATCACaa                                                                                   >  1:253516/1‑140 (MQ=255)                                                     tGACTAATGCCGCCATTGACAGAATATACGGAATGATATTGGTCACCACGGCCAGGTTAACCAGCACGTTGAACTGGCTGTTTAGCGACGGGCTAATGGTCATCAGTGCCAATCCACTCTGAATAATCACAATGGTCAAc                                                                           <  2:397016/140‑1 (MQ=255)                                                          aaTGCCGCCATTGACAGAATATACGGAATGATATTGGTCACCACGGCCAGGTTAACCAGCACGTTGAACTGGCTGTTTAGCGACGGGCTAATGGTCATCAGTGCCAATCCACTCTGAATAATCACAATGGTCAACATTcc                                                                      >  1:22948/1‑140 (MQ=255)                                                            tGCCGCCATTGACAGAATATACGGAATGATATTGGTCACCACGGCCAGGTTAACCAGCACGTTGAACTGGCTGTTTAGCGACGGGCTAATGGTCATCAGTGCCAATCCACTCTGAATAATCACAATGGTCAACATTCCCt                                                                    <  1:630563/140‑1 (MQ=255)                                                                gccATTGACAGAATATACGGAATGATATTGGTCACCACGGCCAGGTTAACCAGCACGTTGAACTGGCTGTTTAGCGACGGGCTAATGGTCATCAGTGCCAATCCACTCTGAATAATCACAATGGTCAACATTCCCTGCAc                                                                >  1:855648/1‑140 (MQ=255)                                                                 ccATTGACAGAATATACGGAATGATATTGGTCACCACGGCCAGGTTAACCAGCACGTTGAACTGGCTGTTTAGCGACGGGCTAATGGTCATCAGTGCCAATCCACTCTGAATAATCACAATGGTCAACATTCCCTGCAcc                                                               >  1:98777/1‑140 (MQ=255)                                                                         aGAATATACGGAATGATATTGGTCACCACGGCCAGGTTAACCAGCACGTTGAACTGGCTGTTTAGCGACGGGCTAATGGTCATCAGTGCCAATCCACTCTGAATAATCACAATGGTCAACATTCCCTGCACCGGTGCATc                                                       <  2:98777/140‑1 (MQ=255)                                                                             tataCGGAATGATATTGGTCACCACGGCCAGGTTAACCAGCACGTTGAACTGGCTGTTTAGCGACGGGCTAATGGTCATCAGTGCCAATCCACTCTGAATAATCACAATGGTCAACATTCCCTGCACCGGTGCATCCACt                                                   <  2:22948/140‑1 (MQ=255)                                                                                aCGGAATGATATTGGTCACCACGGCCAGGTTAACCAGCACGTTGAACTGGCTGTTTAGCGACGGGCTAATGGTCATCAGTGCCAATCCACTCTGAATAATCACAATGGTCAACATTCCCTGCACCGGTGc                                                          <  2:239800/130‑1 (MQ=255)                                                                                aCGGAATGATATTGGTCACCACGGCCAGGTTAACCAGCACGTTGAACTGGCTGTTTAGCGACGGGCTAATGGTCATCAGTGCCAATCCACTCTGAATAATCACAATGGTCAACATTCCCTGCACCGGTGc                                                          >  1:239800/1‑130 (MQ=255)                                                                                   gAATGATATTGGTCACCACGGCCAGGTTAACCAGCACGTTGAACTGGCTGTTTAGCGACGGGCTAATGGTCATCAGTGCCAATCCACTCTGAATAATCACAATGGTCAACATTCCCTGCACCGGTGCATCCACTTTGGtt                                             >  1:475423/1‑140 (MQ=255)                                                                                      tGATATTGGTCACCACGGCCAGGTTAACCAGCACGTTGAACTGGCTGTTTAGCGACGGGCTAATGGTCATCAGTGCCAATCCACTCTGAATAATCACAATGGTCAACATTCCCTGCACCGGTGCATCCACTTTGGTTaca                                          >  2:433233/1‑140 (MQ=255)                                                                                             ggTCACCACGGCCAGGTTAACCAGCACGTTGAACTGGCTGTTTAGCGACGGGCTAATGGTCATCAGTGCCAATCCACTCTGAATAATCACAATGGTCAACATTCCCTGCACCGGTGCATCCACTTTGGTTACACGGgaga                                   <  2:855648/140‑1 (MQ=255)                                                                                             ggTCACCACGGCCAGGTTAACCAGCACGTTGAACTAGCTGTTTAGCGACGGGCTAATGGTCATCAGTGCCAATCCACTCTGAATAATCACAATGGTCAACATTCCCTGCACCGGTGCATCCACTTTGGTTACACGGgaga                                   <  1:237450/140‑1 (MQ=255)                                                                                              gTCACCACGGCCAGGTTAACCAGCACGTTGAACTGGCTGTTTAGCGACGGGCTAATGGTCATCAGTGCCAATCCACTCTGAATAATCACAATGGTCAACATTCCCTGCACCGGTGCATCCAc                                                    <  2:678497/122‑1 (MQ=255)                                                                                              gTCACCACGGCCAGGTTAACCAGCACGTTGAACTGGCTGTTTAGCGACGGGCTAATGGTCATCAGTGCCAATCCACTCTGAATAATCACAATGGTCAACATTCCCTGCACCGGTGCATCCAc                                                    >  1:678497/1‑122 (MQ=255)                                                                                              gTCACCACGGCCAGGTTAACCAGCACGTTGAACTGGCTGTTTAGCGACGGGCTAATGGTCATCAGTGCCAATCCACTCTGAATAATCACAATGGTCAACATTCCCTGCACCGGTGCATCCACTTTGGTTACACGGGAGaa                                  >  2:471164/1‑140 (MQ=255)                                                                                                caccacGGCCAGGTTAACCAGCACGTTGAACTGGCTGTTTAGCGACGGGCTAATGGTCATCAGTGCCAATCCACTCTGAATAATCACAATGGTCAACATTCCCTGCACCGGTGCATCCACTTTGGTTACACGGGAGaaaa                                >  2:200565/1‑140 (MQ=255)                                                                                                  ccacGGCCAGGTTAACCAGCACGTTGAACTGGCTGTTTAGCGACGGGCTAATGGTCATCAGTGCCAATCCACTCTGAATAATCACAATGGTCAACATTCCCTGCACCGGTGCATCCACTTTGGTTACACGGGAGAAAAtt                              >  1:671914/1‑140 (MQ=255)                                                                                                   cacGGCCAGGTTAACCAGCACGTTGAACTGGCTGTTTAGCGACGGGCTAATGGTCATCAGTGCCAATCCACTCTGAATAATCACAATGGTCAACATTCCCTGCACCGGTGCATCCACTTTGGTTACACGGGAGAAAAttt                             >  1:362923/1‑140 (MQ=255)                                                                                                     cGGCCAGGTTAACCAGCACGTTGAACTGGCTGTTTAGCGACGGGCTAATGGTCATCAGTGCCAATCCACTCTGAATAATCACAATGGTCAACATTCCCTGCACCGGTGCATCCACTTTGGTTACACGGGAGAAAATTTTa                           <  1:714650/140‑1 (MQ=255)                                                                                                               aaCCAGCACGTTGAACTGGCTGTTTAGCGACGGGCTAATGGTCATCAGTGCCAATCCACTCTGAATAATCACAATGGTCAACATTCCCTGCACCGGTGCATCCACTTTGGTTACACGGGAGAAAATTTTAGGGAAGTAGc                 <  2:362923/140‑1 (MQ=255)                                                                                                                    gCACGTTGAACTGGCTGTTTAGCGACGGGCTAATGGTCATCAGTGCCAATCCTCTCTGAATAATCACAATGGTCAACATTCCCTGCACCGGTGCATCCACTTTGGTTACACGGGAGAAAATTTTAGGGAAGTAGCCTtca            <  2:254452/140‑1 (MQ=255)                                                                                                                     cACGTTGAACTGGCTGTTTAGCGACGGGCTAATGGTCATCAGTGCCAATCCACTCTGAATAATCACAATGGTCAACATTCCCTGCACCGGTGCATCCACTTTGGTTACACGGGAGAAAATTTTAGGGAAGTAGCCTtcat           >  1:771800/1‑140 (MQ=255)                                                                                                                      aCGTTGAACTGGCTGTTTAGCGACGGGCTAATGGTCATCAGTGCCAATCCACTCTGAATAATCACAATGGTCAACATTCCCTGCACCGGTGCATCCACTTTGGTTACACGGGAGAAAATTTTAGGGAAGTAGCCTtcatc          >  2:308876/1‑140 (MQ=255)                                                                                                                      aCGTTGAACTGGCTGTTTAGCGACGGGCTAATGGTCATCAGTGCCAATCCACTCTGAATAATCACAATGGTCAACATTCCCTGCACCGGTGCATCCACTTTGGTTACACGGGAGAAAATTTTAGGGAAGTAGCCTtca            >  2:667460/1‑138 (MQ=255)                                                                                                                      aCGTTGAACTGGCTGTTTAGCGACGGGCTAATGGTCATCAGTGCCAATCCACTCTGAATAATCACAATGGTCAACATTCCCTGCACCGGTGCATCCACTTTGGTTACACGGGAGAAAATTTTAGGGAAGTAGCCTtca            <  1:667460/138‑1 (MQ=255)                                                                                                                          tGAACTGGCTGTTTAGCGACGGGCTAATGGTCATCAGTGCCAATCCACTCTGAATAATCACAATGGTCAACATTCCCTGCACCGGTGCATCCACTTTGGTTACACGGGAGAAAATTTTAGGGAAGTAGCCTTCATCAGAt      >  2:374325/1‑140 (MQ=255)                                                                                                                          tGAACTGGCTGTTTAGCGACGGGCTAATGGTCATCAGTGCCAATCCACTCTGAATAATCACAATGGTCAACATTCCCTGCACCGGTGCATCCACTTTGGTTACACGGGAGAAAATTTTAGGGAAGTAGCCTTCATCAGAt      >  1:808446/1‑140 (MQ=255)                                                                                                                           gAACTGGCTGTTTAGCGACGGGCTAATGGTCATCAGTGCCAATCCACTCTGAATAATCACAATGGTCAACATTCCCTGCACCGGTGCATCCACTTTGGTTACACGGGAGAAAATTTTAGGGAAGTAGCCTTCATCAGATg     <  1:835811/140‑1 (MQ=255)                                                                                                                           gAACTGGCTGTTTAGCGACGGGCTAATGGTCATCAGTGCCAATCCACTCTGAATAATCACAATGGTCAACATTCCCTGCACCGGTGCATCCACTTTGGTTACACGGGAGAAAATTTTAGGGAAGTAGCCTTCATCAGATg     <  2:808446/140‑1 (MQ=255)                                                                                                                              ctggctgTTTAGCGACGGGCTAATGGTCATCAGTGCCAATCCACTCTGAATAATCACAATGGTCAACATTCCCTGCACCGGTGCATCCACTTTGGTTACACGGGAGAAAATTTTAGGGAAGTAGCCTTCATCAGATGAAg  <  1:779858/140‑1 (MQ=255)                                                                                                                                 |                                                                                                                                         AAGTTTGCAACTTTCGCTTTTGAGGGGGGCACATTAGCGACCTTCTGAATAATGACTAATGCCGCCATTGACAGAATATACGGAATGATATTGGTCACCACGGCCAGGTTAACCAGCACGTTGAACTGACTGTTTAGCGACGGGCTAATGGTCATCAGTGCCAATCCACTCTGAATAATCACAATGGTCAACATTCCCTGCACCGGTGCATCCACTTTGGTTACACGGGAGAAAATTTTAGGGAAGTAGCCTTCATCAGATGAAG  >  NC_000913/717097‑717361

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑