Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,159,176	T→G	D436E (GAT→GAG)	ptsG →	glucose‑specific PTS enzyme IIBC component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,159,176	0	T	G	100.0%	164.3 / NA	45	D436E (GAT→GAG)	ptsG	glucose‑specific PTS enzyme IIBC component
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (22/23);  total (22/23)

AGCGACAGGTACCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAACATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCACTA  >  NC_000913/1159050‑1159301                                                                                                                               |                                                                                                                              aGCGACAGGTACCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAACATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGAGCAGGCCGGCCTga                                                                                                                  >  2:283310/1‑140 (MQ=255)            ccAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAACATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGAGCAGGCCGGCCTGAAGAAACTGGgc                                                                                                       <  1:778478/140‑1 (MQ=255)                  aaaTGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAACATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGAGCAGGCCGGCCTGAAGAAACTGGGCGCAGCg                                                                                                 >  2:422228/1‑140 (MQ=255)                         aCCGGCTCTGGTTACTGCATTTGGTGGTAAAGAAAACATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGAGCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAg                                                                                          >  1:539229/1‑140 (MQ=255)                             gCTCTGGTTGCTGCATTTGGTGGTAAAGAAAACATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGAGCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGt                                                                                      >  1:659483/1‑140 (MQ=255)                                      gctgcATTTGGTGGTAAAGAAAACATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGAGCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTc                                                                             <  2:50728/140‑1 (MQ=255)                                        tgcATTTGGTGGTAAAGAAAACATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGAGCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTg                                                                           >  2:462173/1‑140 (MQ=255)                                           aTTTGGTGGTAAAGAAAACATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGAGCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGgtg                                                                        >  1:652819/1‑140 (MQ=255)                                                 tggtAAAGAAAACATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGAGCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAgg                                                                  <  2:652819/140‑1 (MQ=255)                                                    tAAAGAAAACATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGAGCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGa                                                               >  2:95836/1‑140 (MQ=255)                                                    tAAAGAAAACATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGAGCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGa                                                               >  2:704514/1‑140 (MQ=255)                                                     aaaGAAAACATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGAGCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGAt                                                              <  1:422228/140‑1 (MQ=255)                                                              aTTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGAGCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTAc                                                     >  2:478440/1‑140 (MQ=255)                                                                tACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGAGCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTa                                                   >  2:209044/1‑140 (MQ=255)                                                                 aCTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGAGCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTaa                                                  <  1:704514/140‑1 (MQ=255)                                                                      ccTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGAGCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCg                                             >  2:166430/1‑140 (MQ=255)                                                                          gACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGAGCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATaa                                         >  2:851337/1‑140 (MQ=255)                                                                          gACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGAGCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATa                                          <  2:822715/139‑1 (MQ=255)                                                                          gACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGAGCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATa                                          >  1:822715/1‑139 (MQ=255)                                                                               aTGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGAGCAAGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGa                                    >  1:192932/1‑140 (MQ=255)                                                                                     tACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGAGCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCg                              <  1:283310/140‑1 (MQ=255)                                                                                       cccGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGAGCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCgag                            <  1:462173/140‑1 (MQ=255)                                                                                        ccGTCTGCGCGTCAGTGTTGCTGATGTGTCTAAAGTGGAGCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCgaga                           <  1:472876/140‑1 (MQ=255)                                                                                          gTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGAGCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAAc                                >  1:719154/1‑133 (MQ=255)                                                                                          gTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGAGCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAAc                                <  2:719154/133‑1 (MQ=255)                                                                                          gTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGAGCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCgaga                           <  1:262304/138‑1 (MQ=255)                                                                                          gTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGAGCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCgaga                           >  2:262304/1‑138 (MQ=255)                                                                                          gTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGAGCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAgatg                         >  1:591947/1‑140 (MQ=255)                                                                                           tCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGAGCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAgatgg                        <  1:851337/140‑1 (MQ=255)                                                                                           tCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGAGCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAgatgg                        >  1:308016/1‑140 (MQ=255)                                                                                           tCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGAGCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAgatgg                        <  1:209044/140‑1 (MQ=255)                                                                                            cTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGAGCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGaa                                   >  1:509078/1‑128 (MQ=255)                                                                                            cTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGAGCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGaa                                   <  2:509078/128‑1 (MQ=255)                                                                                            cTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGAGCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAgatgga                       <  1:333782/140‑1 (MQ=255)                                                                                             tGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGAGCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAgatggat                      <  2:276299/140‑1 (MQ=255)                                                                                             tGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGAGCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAgatggat                      <  1:651470/140‑1 (MQ=255)                                                                                                     aGCGTTGCTGATGTGTCTAAAGTGGAGCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAgatggat                      >  2:416753/1‑132 (MQ=255)                                                                                                     aGCGTTGCTGATGTGTCTAAAGTGGAGCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAgatggat                      <  1:416753/132‑1 (MQ=255)                                                                                                        gTTGCTGATGTGTCTAAAGTGGAGCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCg           <  2:659483/140‑1 (MQ=255)                                                                                                         ttGCTGATGTGTCTAAAGTGGAGCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGt          >  2:440697/1‑140 (MQ=255)                                                                                                         ttGCTGATGTGTCTAAAGTGGAGCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGt          <  1:95836/140‑1 (MQ=255)                                                                                                         ttGCTGATGTGTCTAAAGTGGAGCAAGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGt          <  2:192932/140‑1 (MQ=255)                                                                                                                 gtgtCTAAAGTGGAGCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCACTa  <  1:478440/140‑1 (MQ=255)                                                                                                                    tCTAAAGTGGAGCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGt          >  2:503375/1‑129 (MQ=255)                                                                                                                    tCTAAAGTGGAGCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGt          <  1:503375/129‑1 (MQ=255)                                                                                                                               |                                                                                                                              AGCGACAGGTACCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAACATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCACTA  >  NC_000913/1159050‑1159301

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑