Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,236,892	G→T	A117E (GCG→GAG)	ycgB ←	PF04293 family protein YcgB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,236,892	0	G	T	100.0%	131.8 / NA	38	A117E (GCG→GAG)	ycgB	PF04293 family protein YcgB
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (14/24);  total (14/24)

TCTTCGCACTCGGTAATATATTTACGGGCAAAAATCAGATAATCGACAATCGAACTGGCGTCGGTCCAGCTACGGAATAAGTAATTGTTTTTGAAGAAAGAGTTATGCCCATAGCAGGCATGAGCCATCACCAGCGCTTGCATGGTAATGGTGTTCTCTTCCATCAGGTAAGCGATACACGGGTTAGAGTTAATGACGATTTCATAGGCCAGTCCTTGCTGACCGTGCTTATACAGCCGTTCAGTCTCGATAAACTTTTTA  >  NC_000913/1236757‑1237017                                                                                                                                        |                                                                                                                              tctTCGCACTCGGTAATATATTTACGGGCAAAAATCAGATAATCGACAATCGAACTGGCGTCGGTCCAGCTACGGAATAAGTAATTGTTTTTGAAGAAAGAGTTATGCCCATAGCAGGCATGAGCCATCACCAGCTCTTg                                                                                                                           >  2:572144/1‑140 (MQ=255) tctTCGCACTCGGTAATATATTTACGGGCAAAAATCAGATAATCGACAATCGAACTGGCGTCGGTCCAGCTACGGAATAAGTAATTGTTTTTGAAGAAAGAGTTATGCCCATAGCAGGCATGAGCCATCACCAGCTCTTg                                                                                                                           <  1:76505/140‑1 (MQ=255)   ttCGCACTCGGTAATATATTTACGGGCAAAAATCAGATAATCGACAATCGAACTGGCGTCGGTCCAGCTACGGAATAAGTAATTGTTTTTGAAGAAAGAGTTATGCCCATAGCAGGCATGAGCCATCACCAGCTCTTGCa                                                                                                                         <  2:758671/140‑1 (MQ=255)   ttCGCACTCGGTAATATATTTACGGGCAAAAATCAGATAATCGACAATCGAACTGGCGTCGGTCCAGCTACGGAATAAGTAATTGTTTTTGAAGAAAGAGTTATGCCCATAGCAGGCATGAGCCATCACCAGCTCTTGCa                                                                                                                         <  1:572144/140‑1 (MQ=255)    tCGCACTCGGTAATATATTTACGGGCAAAAATCAGATAATCGACAATCGAACTGGCGTCGGTCCAGCTACGGAATAAGTAATTGTTTTTGAAGAAAGAGTTATGCCCATAGCAGGCATGAGCCATCACCAGCTCTTGCAt                                                                                                                        <  2:836908/140‑1 (MQ=255)      gCACTCGGTAATATATTTACGGGCAAAAATCAGATAATCGACAATCGAACTGGCGTCGGTCCAGCTACGGAATAAGTAATTGTTTTTGAAGAAAGAGTTATGCCCATAGCAGGCATGAGCCATCACCAGCTCTTGCATgg                                                                                                                      <  2:55743/140‑1 (MQ=255)             gTAATATATTTACGGGCAAAAATCAGATAATCGACAATCGAACTGGCGTCGGTCCAGCTACGGAATAAGTAATTGTTTTTGAAGAAAGAGTTATGCCCATAGCAGGCATGAGCCATCACCAGCTCTTGCAt                                                                                                                        <  2:391585/131‑1 (MQ=255)             gTAATATATTTACGGGCAAAAATCAGATAATCGACAATCGAACTGGCGTCGGTCCAGCTACGGAATAAGTAATTGTTTTTGAAGAAAGAGTTATGCCCATAGCAGGCATGAGCCATCACCAGCTCTTGCAt                                                                                                                        >  1:391585/1‑131 (MQ=255)             gTAATATATTTACGGGCAAAAATCAGATAATCGACAATCGAACTGGCGTCGGTCCAGCTACGGAATAAGTAATTGTTTTTGAAGAAAGAGTTATGCCCATAGCAGGCATGAGCCATCACCAGCTCTTGCATGGTAATGgt                                                                                                               >  1:388795/1‑140 (MQ=255)               aaTATATTTACGGGCAAAAATCAGATAATCGACAATCGAACTGGCGTCGGTCCAGCTACGGAATAAGTAATTGTTTTTGAAGAAAGAGTTATGCCCATAGCAGGCATGAGCCATCACCAGCTCTTGCATGGTAATGgtgt                                                                                                             <  2:192821/140‑1 (MQ=255)                  atatTTACGGGCAAAAATCAGATAATCGACAATCGAACTGGCGTCGGTCCAGCTACGGAATAAGTAATTGTTTTTGAAGAAAGAGTTATGCCCATAGCAGGCATGAGCCATCACCAGCTCTTGCATGGTAATGGTGTtct                                                                                                          <  2:431341/140‑1 (MQ=255)                    atTTACGGGCAAAAATCAGATAATCGACAATCGAACTGGCGTCGGTCCAGCTACGGAATAAGTAATTGTTTTTGAAGAAAGAGTTATGCCCATAGCAGGCATGAGCCATCACCAGCTCTTGCATGGTAATGGTGTTctct                                                                                                        >  1:223056/1‑140 (MQ=255)                      ttACGGGCAAAAATCAGATAATCGACAATCGAACTGGCGTCGGTCCAGCTACGGAATAAGTAATTGTTTTTGAAGAAAGAGTTATGCCCATAGCAGGCATGAGCCATCACCAGCTCTTGCATGGTAATGGTGTTCTCTTc                                                                                                      >  1:163269/1‑140 (MQ=255)                        aCGGGCAAAAATCAGATAATCGACAATCGAACTGGCGTCGGTCCAGCTACGGAATAAGTAATTGTTTTTGAAGAAAGAGTTATGCCCATAGCAGGCATGAGCCATCACCAGCTCTTGCATGGTAATGGTGTTCTCTTCCa                                                                                                    >  1:680669/1‑140 (MQ=255)                           ggCAAAAATCAGATAATCGACAATCGAACTGGCGTCGGTCCAGCTACGGAATAAGTAATTGTTTTTGAAGAAAGAGTTATGCCCATAGCAGGCATGAGCCATCACCAGCTCTTGCATGGTAATGGTGTTCTCTTCCATc                                                                                                  <  2:859451/139‑1 (MQ=255)                           ggCAAAAATCAGATAATCGACAATCGAACTGGCGTCGGTCCAGCTACGGAATAAGTAATTGTTTTTGAAGAAAGAGTTATGCCCATAGCAGGCATGAGCCATCACCAGCTCTTGCATGGTAATGGTGTTCTCTTCCATc                                                                                                  >  1:859451/1‑139 (MQ=255)                             cAAAAATCAGATAATCGACAATCGAACTGGCGTCGGTCCAGCTACGGAATAAGTAATTGTTTTTGAAGAAAGAGTTATGCCCATAGCAGGCATGAGCCATCACCAGCTCTTGCATGGTAATGGTGTTCTCTTcc                                                                                                     <  1:746854/134‑1 (MQ=255)                             cAAAAATCAGATAATCGACAATCGAACTGGCGTCGGTCCAGCTACGGAATAAGTAATTGTTTTTGAAGAAAGAGTTATGCCCATAGCAGGCATGAGCCATCACCAGCTCTTGCATGGTAATGGTGTTCTCTTcc                                                                                                     >  2:746854/1‑134 (MQ=255)                              aaaaaTCAGATAATCGACAATCGAACTGGCGTCGGTCCAGCTACGGAATAAGTAATTGTTTTTGAAGAAAGAGTTATGTCCATAGCAGGCATGAGCCATCACCAGCTCTTGCATGGTAATGGTGTTCTCTTCCATCAGGt                                                                                              <  2:554147/140‑1 (MQ=255)                              aaaaaTCAGATAATCGACAATCGAACTGGCGTCGGTCCAGCTACGGAATAAGTAATTGTTTTTGAAGAAAGAGTTATGCCCATAGCAGGCATGAGCCATCACCAGCTCTTGCATGGTAATGGTGTTCTCTTCCATCAGGt                                                                                              <  1:807270/140‑1 (MQ=255)                                 aaTCAGATAATCGACAATCGAACTGGCGTCGGTCCAGCTACGGAATAAGTAATTGTTTTTGAAGAAAGAGTTATGCCCATAGCAGGCATGAGCCATCACCAGCTCTTGCATGGTAATGGTGTTCTCTTCCATCAGGTAAg                                                                                           <  2:547619/140‑1 (MQ=255)                                       aTAATCGACAATCGAACTGGCGTCGGTCCAGCTACGGAATAAGTAATTGTTTTTGAAGAAAGAGTTATGCCCATAGCAGGCATGAGCCATCACCAGCTCTTGCATGGTAATGGTGTTCTCTTCCATCAGGTAAGCGATac                                                                                     <  2:680669/140‑1 (MQ=255)                                        tAATCGACAATCGAACTGGCGTCGGTCCAGCTACGGAATAAGTAATTGTTTTTGAAGAAAGAGTTATGCCCATAGCAGGCATGAGCCATCACCAGCTCTTGCATGGTAATGGTGTTCTCTTCCATCAGGTAAGCGATaca                                                                                    <  1:667816/140‑1 (MQ=255)                                        tAATCGACAATCGAACTGGCGTCGGTCCAGCTACGGAATAAGTAATTGTTTTTGAAGAAAGAGTTATGCCCATAGCAGGCATGAGCCATCACCAGCTCTTGCATGGTAATGGTGTTCTCTTCCATCAGGTAAGCGATaca                                                                                    <  2:223056/140‑1 (MQ=255)                                        tAATCGACAATCGAACTGGCGTCGGTCCAGCTACGGAATAAGTAATTGTTTTTGAAGAAAGAGTTATGCCCATAGCAGGCATGAGCCATCACCAGCTCTTGCATGGTAATGGTGTTCTCTTCCATCAGGTAAGCGATaca                                                                                    <  2:328535/140‑1 (MQ=255)                                              aCAATCGAACTGGCGTCGGTCCAGCTACGGAATAAGTAATTGTTTTTGAAGAAAGAGTTATGCCCATAGCAGGCATGAGCCATCACCAGCTCTTGCATGGTAATGGTGTTCTCTTCCATCAGGTAAGCGATACACGGGtt                                                                              <  1:150772/140‑1 (MQ=255)                                                     aaCTGGCGTCGGTCCAGCTACGGAATAAGTAATTGTTTTTGAAGAAAGAGTTATGCCCATAGCAGGCATGAGCCATCACCAGCTCTTGCATGGTAATGGTGTTCTCTTCCATCAGGTAAGCGATacac                                                                                   >  2:499451/1‑128 (MQ=255)                                                     aaCTGGCGTCGGTCCAGCTACGGAATAAGTAATTGTTTTTGAAGAAAGAGTTATGCCCATAGCAGGCATGAGCCATCACCAGCTCTTGCATGGTAATGGTGTTCTCTTCCATCAGGTAAGCGATacac                                                                                   <  1:499451/128‑1 (MQ=255)                                                                gTCCAGCTACGGAATAAGTAATTGTTTTTGAAGAAAGAGTTATGCCCATAGCAGGCATGAGCCATCACCAGCTCTTGCATGGTAATGGTGTTCTCTTCCATCAGGTAAGCGATACACGGGTTAGAGTTAATGACGATTTc                                                            >  2:35731/1‑140 (MQ=255)                                                                 tCCAGCTACGGAATAAGTAATTGTTTTTGAAGAAAGAGTTATGCCCATAGCAGGCATGAGCCATCACCAGCTCTTGCATGGTAATGGTGTTCTCTTCCATCAGGTAAGCGATACACGGGTTAGAGTTAATGACGATTTCa                                                           <  2:20591/140‑1 (MQ=255)                                                                    aGCTACGGAATAAGTAATTGTTTTTGAAGAAAGAGTTATGCCCATAGCAGGCATGAGCCATCACCAGCTCTTGCATGGTAATGGTGTTCTCTTCCATCAGGTAAGCGATACACGGGTTAGAGTTAATGACGATTTCATAg                                                        >  2:102189/1‑140 (MQ=255)                                                                           gAATAAGTAATTGTTTTTGAAGAAAGAGTTATGCCCATAGCAGGCATGAGCCATCACCAGCTCTTGCATGGTAATGGTGTTCTCTTCCATCAGGTAAGCGATACACGGGTTAGAGTTAATGACGATTTCATAGGCCAGTc                                                 <  2:414021/140‑1 (MQ=255)                                                                                    aTTGTTTTTGAAGAAAGAGTTATGCCCATAGCAGGCATGAGCCATCACCAGCTCTTGCATGGTAATGGTGTTCTCTTCCATCAGGTAAGCGATACACGGGTTAGAGTTAATGACGATTTCATAGGCCAGTCCTTGCTGAc                                        <  1:35731/140‑1 (MQ=255)                                                                                                 aaagaGTTATGCCCATAGCAGGCATGAGCCATCACCAGCTCTTGCATGGTAATGGTGTTCTCTTCCATCAGGTAAGCGATCCACGGGTTAGAGTTAATGACGATTTCATAGGCCAGTCCTGGCTGACCGTGCTTATACAg                           <  2:556988/140‑1 (MQ=255)                                                                                                               aTAGCAGGCATGAGCCATCACCAGCTCTTGCATGGTAATGGTGTTCTCTTCCATCAGGTAAGCGATACACGGGTTAGAGTTAATGACGATTTCATAGGCCAGTCCTTGCTGACCGTGCTTATACAGCCGTTCAGTCTCGa             >  1:324796/1‑140 (MQ=255)                                                                                                                          gAGCCATCACCAGCTCTTGCATGGTAATGGTGTTCTCTTCCATCAGGTAAGCGATACACGGGTTAGAGTTAATGACGATTTCATAGGCCAGTCCTTGCTGACCGTGCTTATACAGCCGTTCAGTCTCGATAAACTTTTTa  >  1:140562/1‑140 (MQ=255)                                                                                                                           aGCCATCACCAGCTCTTGCATGGTAATGGTGTTCTCTTCCATCAGGTAAGCGATACACGGGTTAGAGTTAATGACGATTTCATAGGCCAGTCCTTGCTGACCGTGCTTATACAGCCGTTCAGTCTCGAt            >  2:32389/1‑129 (MQ=255)                                                                                                                           aGCCATCACCAGCTCTTGCATGGTAATGGTGTTCTCTTCCATCAGGTAAGCGATACACGGGTTAGAGTTAATGACGATTTCATAGGCCAGTCCTTGCTGACCGTGCTTATACAGCCGTTCAGTCTCGAt            <  1:32389/129‑1 (MQ=255)                                                                                                                                        |                                                                                                                              TCTTCGCACTCGGTAATATATTTACGGGCAAAAATCAGATAATCGACAATCGAACTGGCGTCGGTCCAGCTACGGAATAAGTAATTGTTTTTGAAGAAAGAGTTATGCCCATAGCAGGCATGAGCCATCACCAGCGCTTGCATGGTAATGGTGTTCTCTTCCATCAGGTAAGCGATACACGGGTTAGAGTTAATGACGATTTCATAGGCCAGTCCTTGCTGACCGTGCTTATACAGCCGTTCAGTCTCGATAAACTTTTTA  >  NC_000913/1236757‑1237017

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑