Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,632,895	G→A	G392G (GGC→GGT)	guaB ←	inosine 5'‑monophosphate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,632,895	0	G	A	100.0%	162.5 / NA	47	G392G (GGC→GGT)	guaB	inosine 5'‑monophosphate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (23/24);  total (23/24)

CGCCCATCTGCTGGTGAATGATCTCTTTCAGGCGACCTTTATAGGCTACGCGACCTTCGATACCTTCCGGCACCAGTTTGTCGGCAGCGTTATCGCTCTGGAAATAACGGTCAGAGGAACCTTTGGACATCGCGCCCAGGGAACCCATACCACGGTAAGATTTGTAAGAACGGCCCTGGTAGAGTTCGATTTCACCCGGAGATTCTTCAGTACCCGCCAGCATGGAACCTACCATCACCGCGCTTGCGCCAGCGGCGATAGCTT  >  NC_000913/2632762‑2633025                                                                                                                                      |                                                                                                                                   cgccCATCTGCTGGTGAATGATCTCTTTCAGGCGACCTTTATAGGCTACGCGACCTTCGATACCTTCCGGCACCAGTTTGTCGGCAGCGTTATCGCTCTGGAAATAACGGTCAGAGGAACCTTTGGACATCGCACCCAgg                                                                                                                              >  1:1044127/1‑140 (MQ=255) cgccCATCTGCTGGTGAATGATCTCTTTCAGGCGACCTTTATAGGCTACGCGACCTTCGATACCTTCCGGCACCAGTTTGTCGGCAGCGTTATCGCTCTGGAAATAACGGTCAGAGGAACCTTTGGACATCGCACCCAgg                                                                                                                              <  2:383852/140‑1 (MQ=255) cgccCATCTGCTGGTGAATGATCTCTTTCAGGCGACCTTTATAGGCTACGCGACCTTCGATACCTTCCGGCACCAGTTTGTCGGCAGCGTTATCGCTCTGGAAATAACGGTCAGAGGAACCTTTGGACATCGCACCCAgg                                                                                                                              <  2:423443/140‑1 (MQ=255) cgccCATCTGCTGGTGAATGATCTCTTTCAGGCGACCTTTATAGGCTACGCGACCTTCGATACCTTCCGGCACCAGTTTGTCGGCAGCGTTATCGCTCTGGAAATAACGGTCAGAGGAACCTTTGGACATCGCACCCAgg                                                                                                                              <  2:829663/140‑1 (MQ=255) cgccCATCTGCTGGTGAATGATCTCTTTCAGGCGACCTTTATAGGCTACGCGACCTTCGATACCTTCCGGCACCAGTTTGTCGGCAGCGTTATCGCTCTGGAAATAACGGTCAGAGGAACCTTTGGACATCGCACCCAgg                                                                                                                              <  2:1328424/140‑1 (MQ=255)  gccCATCTGCTGGTGAATGATCTCTTTCAGGCGACCTTTATAGGCTACGCGACCTTCGATACCTTCCGGCACCAGTTTGTCGGCAGCGTTATCGCTCTGGAAATAACGGTCAGAGGAACCTTTGGACATCGCACCCAggg                                                                                                                             <  2:967933/140‑1 (MQ=255)     cATCTGCTGGTGAATGATCTCTTTCAGGCGACCTTTATAGGCTACGCGACCTTCGATACCTTCCGGCACCAGTTTGTCGGCAGCGTTATCGCTCTGGAAATAACGGTCAGAGGAACCTTTGGACATCGCACCCAGGGAAc                                                                                                                          <  2:52736/140‑1 (MQ=255)           ctgGTGAATGATCTCTTTCAGGCGACCTTTATAGGCTACGCGACCTTCGATACCTTCCGGCACCAGTTTGTCGGCAGCGTTATCGCTCTGGAAATAACGGTCAGAGGAACCTTTGGACATCGCACCCAGGGAACCCATAc                                                                                                                    <  2:453398/140‑1 (MQ=255)            tgGTGAATGATCTCTTTCAGGCGACCTTTATAGGCTACGCGACCTTCGATACCTTCCGGCACCAGTTTGTCGGCAGCGTTATCGCTCTGGAAATAACGGTCAGAGGAACCTTTGGACATCGCACCCAGGGAACCCATAcc                                                                                                                   <  2:36632/140‑1 (MQ=255)                gAATGATCTCTTTCAGGCGACCTTTATAGGCTACGCGACCTTCGATACCTTCCGGCACCAGTTTGTCGGCAGCGTTATCGCTCTGGAAATAACGGTCAGAGGAACCTTTGGACATCGCACCCAggg                                                                                                                             <  2:651552/126‑1 (MQ=255)                gAATGATCTCTTTCAGGCGACCTTTATAGGCTACGCGACCTTCGATACCTTCCGGCACCAGTTTGTCGGCAGCGTTATCGCTCTGGAAATAACGGTCAGAGGAACCTTTGGACATCGCACCCAGGGAACCCATACCACgg                                                                                                               <  2:630946/140‑1 (MQ=255)                gAATGATCTCTTTCAGGCGACCTTTATAGGCTACGCGACCTTCGATACCTTCCGGCACCAGTTTGTCGGAAGCGTTATCGCTCTGGAAATAACGGTCAGAGGAACCTTTGGACATCGCACCCAggg                                                                                                                             >  1:651552/1‑126 (MQ=255)                   tGATCTCTTTCAGGCGACCTTTATAGGCTACGCGACCTTCGATACCTTCCGGCACCAGTTTGTCGGCAGCGTTATCGCTCTGGAAATAACGGTCAGAGGAACCTTTGGACATCGCACCCAgg                                                                                                                              <  2:751726/122‑1 (MQ=255)                   tGATCTCTTTCAGGCGACCTTTATAGGCTACGCGACCTTCGATACCTTCCGGCACCAGTTTGTCGGCAGCGTTATCGCTCTGGAAATAACGGTCAGAGGAACCTTTGGACATCGCACCCAgg                                                                                                                              >  1:751726/1‑122 (MQ=255)                        tctTTCAGGCGACCTTTATAGGCTACGCGACCTTCGATACCTTCCGGCACCAGTTTGTCGGCAGCGTTATCGCTCTGGAAATAACGGTCAGAGGAACCTTTGGACATCGCACCCAGGGAACCCATACCACGGTaa                                                                                                            <  2:845017/135‑1 (MQ=255)                        tctTTCAGGCGACCTTTATAGGCTACGCGACCTTCGATACCTTCCGGCACCAGTTTGTCGGCAGCGTTATCGCTCTGGAAATAACGGTCAGAGGAACCTTTGGACATCGCACCCAGGGAACCCATACCACGGTaa                                                                                                            >  1:845017/1‑135 (MQ=255)                        tctTTCAGGCGACCTTTATAGGCTACGCGACCTTCGATACCTTCCGGCACCAGTTTGTCGGCAGCGTTATCGCTCTGGAAATAACGGTCAGAGGAACCTTTGGACATCGCACCCAGGGAACCCATACCACGGTAAGAttt                                                                                                       >  1:147359/1‑140 (MQ=255)                           ttCAGGCGACCTTTATAGGCTACGCGACCTTCGATACCTTCCGGCACCAGTTTGTCGGCAGCGTTATCGCTCTGGAAATAACGGTCAGAGGAACCTTTGGACATCGCACCCAGGGAACCCATACCACGGTAAGATTTGTa                                                                                                    >  2:1201881/1‑140 (MQ=255)                                gCGACCTTTATAGGCTACGCGACCTTCGATACCTTCCGGCACCAGTTTGTCGGCAGCGTTATCGCTCTGGAAATAACGGTCAGAGGAACCTTTGGACATCGCACCCAGGGAACCCATACCACGGTAAGATTTGTAAGAAc                                                                                               <  2:534864/140‑1 (MQ=255)                                gCGACCTTTATAGGCTACGCGACCTTCGATACCTTCCGGCACCAGTTTGTCGGCAGCGTTATCGCTCTGGAAATAACGGTCAGAGGAACCTTTGGACATCGCACCCAGGGAACCCATACCACGGTAAGATTTGTAAGAAc                                                                                               <  2:1044127/140‑1 (MQ=255)                                  gACCTTTATAGGCTACGCGACCTTCGATACCTTCCGGCACCAGTTTGTCGGCAGCGTTATCGCTCTGGAAATAACGGTCAGAGGAACCTTTGGACATCGCACCCAGGGAACCCATACCACGGTAAGATTTGTAAGAACgg                                                                                             <  1:1201881/140‑1 (MQ=255)                                    ccTTTATAGGCTACGCGACCTTCGATACCTTCCGGCACCAGTTTGTCGGCAGCGTTATCGCTCTGGAAATAACGGTCAGAGGAACCTTTGGACATCGCACCCAGGGAACCCATACCACGGTAAGATTTGTAAGAACGGcc                                                                                           <  2:1362857/140‑1 (MQ=255)                                    ccTTTATAGGCTACGCGACCTTCGATACCTTCCGGCACCAGTTTGTCGGCAGCGTTATCGCTCTGGAAATAACGGTCAGAGGAACCTTTGGACATCGCACCCAGGGAACCCATACCACGGTAAGATTTGTAAGAACGGcc                                                                                           >  1:1094123/1‑140 (MQ=255)                                              cTACGCGACCTTCGATACCTTCCGGCACCAGTTTGTCGGCAGCGTTATCGCTCTGGAAATAACGGTCAGAGGAACCTTTGGACATCGCACCCAGGGAACCCATACCACGGTAAGATTTGTAAGAACGGCCCTGGTAGAGt                                                                                 >  1:1131607/1‑140 (MQ=255)                                                 cgcgACCTTCGATACCTTCCGGCACCAGTTTGTCGGCAGCGTTATCGCTCTGGAAATAACGGTCAGAGGAACCTTTGGACATCGTACCCAGGAAACCCATACCACGGTAAGATTTGTAAGAACGGCCCTGGTAGAGTTCg                                                                              >  2:748242/1‑140 (MQ=255)                                                 cgcgACCTTCGATACCTTCCGGCACCAGTTTGTCGGCAGCGTTATCGCTCTGGAAATAACGGTCAGAGGAACCTTTGGACATCGCACCCAGGGAACCCATACCACGGTAAGATTTGTAAGAAc                                                                                               <  2:855110/123‑1 (MQ=255)                                                 cgcgACCTTCGATACCTTCCGGCACCAGTTTGTCGGCAGCGTTATCGCTCTGGAAATAACGGTCAGAGGAACCTTTGGACATCGCACCCAGGGAACCCATACCACGGTAAGATTTGTAAGAAc                                                                                               >  1:855110/1‑123 (MQ=255)                                                 cgcgACCTTCGATACCTTCCGGCACCAGTTTGTCGGCAGCGTTATCGCTCTGGAAATAACGGTCAGAGGAACCTTTGGACATCGCACCCAGGGAACCCATACCACGGTAAGATTTGTAAGAACGGCCCTGGTAGAGTTCg                                                                              >  1:1348477/1‑140 (MQ=255)                                                 cgcgACCTTCGATACCTTCCGGCACCAGTTTGTCGGCAGCGTTATCGCTCTGGAAATAACGGTCAGAGGAACCTTTGGACATCGCACCCAGGGAACCCATACCACGGTAAGATTTGTAAGAACGGCCCTGGTAGAGTTCg                                                                              >  1:673590/1‑140 (MQ=255)                                                       cTTCGATACCTTCCGGCACCAGTTTGTCGGCAGCGTTATCGCTCTGGAAATAACGGTCAGAGGAACCTTTGGACATCGCACCCAGGGAACCCATACCACGGTAAGATTTGTAAGAACGGCCCTGGTAGAGTTCGATTTCa                                                                        >  1:731008/1‑140 (MQ=255)                                                          cGATACCTTCCGGCACCAGTTTGTCGGCAGCGTTATCGCTCTGGAAATAACGGTCAGAGGAACCTTTGGACATCGCACCCAGGGAACCCATACCACGGTAAGATTTGTAAGAACGGCCCTGGTAGAGTTCGATTTCAccc                                                                     <  2:1366122/140‑1 (MQ=255)                                                               ccTTCCGGCACCAGTTTGTCGGCAGCGTTATCGCTCTGGAAATAACGGTCAGAGGAACCTTTGGACATCGCACCCAGGGAACCCATACCACGGTAAGATTTGTAAGAACGGCCCTGGTAGAGTTCGATTTCACCCGgaga                                                                <  2:1426025/140‑1 (MQ=255)                                                                   ccGGCACCAGTTTGTCGGCAGCGTTATCGCTCTGGAAATAACGGTCAGAGGAACCTTTGGACATCGCACCCAGGGAACCCATACCACGGTAAGATTTGTAAGAACGGCCCTGGTAGAGTTCGATTTCACCCGGAGAttct                                                            >  1:1455530/1‑140 (MQ=255)                                                                    cGGCACCAGTTTGTCGGCAGCGTTATCGCTCTGGAAATAACGGTCAGAGGAACCTTTGGACATCGCACCCAGGGAACCCATACCACGGTAAGATTTGTAAGAACGGCCCTGGTAGAGTTCGATTTCACCCGGAGAttctt                                                           <  2:8528/140‑1 (MQ=255)                                                                     ggCACCAGTTTGTCGGCAGCGTTATCGCTCTGGAAATAACGGTCAGAGGAACCTTTGGACATCGCACCCAGGGAACCCATACCACGGTAAGATTTGTAAGAACGGCCCTGGCAGAGTTCGATTTCACCCGGAGAttcttc                                                          >  2:739666/1‑140 (MQ=255)                                                                       cACCAGTTTGTCGGCAGCGTTATCGCTCTGGAAATAACGGTCAGAGGAACCTTTGGACATCGCACCCAGGGAACCCATACCACGGTAAGATTTGTAAGAACGGCCCTGGTAGAGTTCGATTTCACCCGGAGATTCTTCAg                                                        >  1:924330/1‑140 (MQ=255)                                                                              ttGTCGGCAGCGTTATCGCTCTGGAAATAACGGTCAGAGGAACCTTTGGACATCGCACCCAGGGAACCCATACCACGGTAAGATTTGTAAGAACGGCCCTGGTAGAGTTCGATTTCCCCCGGAGATTCTTCAGTACCCGc                                                 <  2:1348477/140‑1 (MQ=255)                                                                                   ggCAGCGTAATCGCTCTGGAAAAAACGTACAAAGGAACCTTTGGACATCGCACCAAGGGAACCCATCCCCCGGTAAGATTTGTAACAACGGCCCTGTTAGAGTTCGATTTCCCCCGGAGATTTTTCAGTCCCCGCCAGCa                                            <  2:1131607/140‑1 (MQ=255)                                                                                       gCGTTATCGCTCTGGAAATAACGGTCAGAGGAACCTTTGGACATCGCACCCAGGGAACCCATACCACGGTAAGATTTGTAAGAACGGCCCTGGTAGAGTTCGTTTTCCCCCGGAGATTCTTCAGTACCCGCCAGCATGGa                                        <  2:1094123/140‑1 (MQ=255)                                                                                        cGTTATCGCTCTGGAAATAACGGTCAGAGGATCCTTTGGACATCGCACCCAGGGAACCCATACCGCGGTAAGATTTGTAAGAACGGCCCTGGTAGATTTCGATTTCCCCGGGAGATTCTTCAGTACCCGCCAGCATGGaa                                       <  2:147359/140‑1 (MQ=255)                                                                                                     gAAATAACGGTCAGAGGAACCTTTGGACATCGCACCCAGGGAACCCATACCACGGTAAGATTTGTAAGAACGGCCCTGGTAGAGTTCGATTTCACCCGGAGATTCTTCAGTACCCGCCAGCATGGAACCTACCATCACcg                          >  2:931272/1‑140 (MQ=255)                                                                                                       aaaatcGGTGAGAGTACCCTTTGGCCATCGCCCCGAGGGAACCCATACCAGGATAAGATTTGTAAGAACGGCCCTGAAAGATTTGTTTTCCACCCGGAGTTTCTTCGGGACCCGCCAGCATGGAACCTACCATCACcgcg                        <  2:349594/135‑1 (MQ=255)                                                                                                             ggTCAGAGGAACCTTTGGACATCGCACCCAGGGAACCCATACCACGGTAAGATTTGTAAGAACGGCCCTGGTAGAGTTCGATTTCACCCGGAGATTCTTCAGTACCCGCCAGCATGGAACCTACCATCACCGCGCTTgcg                  >  2:35660/1‑140 (MQ=255)                                                                                                               tCAGAGGAACCTTTGGACATCGCACCCAGGGAACCCATACCACGGTAAGATTTGTAAGAACGGCCCTGGTAGAGTTCGATTTCACCCGGAGATTCTTCAGTACCCGCCAGCATGGAACCTACCATCACCGCGCTTGCGcc                >  2:484928/1‑140 (MQ=255)                                                                                                               tCAGAGGAACCTTTGGACATCGCACCCAGGGAACCCATACCACGGTAAGATTTGTAAGAACGGCCCTGGTAGAGTTCGATTTCACCCGGAGATTCTTCAGTACCCGCCAGCATGGAACCTACCATCACCGCGCTTGCGcc                >  2:249943/1‑140 (MQ=255)                                                                                                                cAGAGAACCCTTTGGACACCGCACCAAGGAACCCCATCCCACGTTAAGATTTGTAAGAACGGCCCGGGTAGAGTTCGATTTCCCCCGGAGATTCTTCAGTACCCGCCAGCATGGAACCTACCATCACCGCGCTTGCGCCa               <  2:731008/140‑1 (MQ=255)                                                                                                                 agagGAACCTTTGGACATCGCACCCAGGGAACCCATACCACGGTAAGATTTGTAAGAACGGCCCTGGTAGAGTTCGATTTCACCCGGAGATTCTTCAGTACCCGCCAGCATGGAACCTACCATCACCGCGCTTGCGCCAg              <  1:788037/140‑1 (MQ=255)                                                                                                                 agagGAACCTTTGGACATCGCACCCAGGGAACCCATACCACGGTAAGATTTGTAAGAACGGCCCTGGTAGAGTTCGATTTCACCCGGAGATTCTTCAGTACCCGCCAGCATGGAACCTACCATCACCGCGCTTGCGCCAg              >  1:349594/1‑140 (MQ=255)                                                                                                                   agGAACCTTTGGACATCGCACCCAGGGAACCCATACCACGGTAAGATTTGTAAGAACGGCCCTGGTAGAGTTCGATTTCACCCGGAGATTCTTCAGTACCCGCCAGCATGGAACCTACCATCACCGCGCTTGCGCCAgcg            >  1:91426/1‑140 (MQ=255)                                                                                                                       cccTTTGGACATCGCCCCCAGGGACCCCATACCACGGTAAGATTTGTAAGAACGGCCCTGGTAGAGTTCGTTTTCCCCCGGAGATTCTTCAGTACCCGCCAGCATGGAACCTACCATCACCGCGCTTGCGCCAGCGGCGa        <  2:673590/139‑1 (MQ=255)                                                                                                                       aCCTTTGGACATCGCACCCAGGGAACCCATACCACGGTAAGATTTGTAAGAACGGCCCTGGTAGAGTTCGATTTCACCCGGAGATTCTTCAGTACCCGCCAGCATGGAACCTACCATCACCGCGCTTGCGCCAGCGGCGa        >  1:740657/1‑140 (MQ=255)                                                                                                                             ggACATCGCACCCAGGGAACCCATACCACGGTAAGATTTGTAAGAACGGCCCTGGTAGAGTTCGATTTCACCCGGAGATTCTTCAGTACCCGCCAGCATGGAACCTACCATCACCGCGCTTGCGCCAGCGGCGATAGCtt  >  1:777902/1‑140 (MQ=255)                                                                                                                                      |                                                                                                                                   CGCCCATCTGCTGGTGAATGATCTCTTTCAGGCGACCTTTATAGGCTACGCGACCTTCGATACCTTCCGGCACCAGTTTGTCGGCAGCGTTATCGCTCTGGAAATAACGGTCAGAGGAACCTTTGGACATCGCGCCCAGGGAACCCATACCACGGTAAGATTTGTAAGAACGGCCCTGGTAGAGTTCGATTTCACCCGGAGATTCTTCAGTACCCGCCAGCATGGAACCTACCATCACCGCGCTTGCGCCAGCGGCGATAGCTT  >  NC_000913/2632762‑2633025

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑