Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,805,710	C→A	A299E (GCA→GAA)	proV →	glycine betaine ABC transporter ATP binding subunit ProV

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,805,710	0	C	A	100.0%	332.1 / NA	93	A299E (GCA→GAA)	proV	glycine betaine ABC transporter ATP binding subunit ProV
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (48/45);  total (48/45)

AATAATCCGGCGAATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGTCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTTAA‑TTCGTAAAACCCCTGGCTTCGGCCCACGTTCGGCACTGAAATTATTGCAGGATGAAGATCGCGAATATGGCTACGTTATCGAACGCGGTAATAAGTTTGTCGGCGCAGTCTCCATCGATTCGCTTAAAACCGCGTTAACGCAGCAGCAAGGTCTTGATGCGGCGCT  >  NC_000913/2805574‑2805842                                                                                                                                          |                                                                                                                                     aataatCCGGCGAATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGTCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTTAA‑TTCGTAAAACCCCTGGCTTCGGCCCACGTTCGGAACt                                                                                                                                   >  2:883378/1‑140 (MQ=255) aataatCCGGCGAATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGTCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTTAA‑TTCGTAAAACCCCTGGCTTCGGCCCACGTTCGGAACt                                                                                                                                   <  2:1060385/140‑1 (MQ=255)   taatCCGGCGAATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGTCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTTAA‑TTCGTAAAACCCCTGGCTTCGGCCCACGTTCGGAACTGa                                                                                                                                 <  1:626470/140‑1 (MQ=255)         ggCGAATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGTCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTTAA‑TTCGTAAAACCCCTGGCTTCGGCCCACGTTCGGAACTGAAattat                                                                                                                           >  1:780876/1‑140 (MQ=255)         ggCGAATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGTCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTTAA‑TTCGTAAAACCCCTGGCTTCGGCCCACGTTCGGAACTG‑Aattatt                                                                                                                          >  1:197543/1‑140 (MQ=255)           cGAATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGTCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTTAA‑TTCGTAAAACCCCTGGCTTCGGCCCACGTTCGGAACTGAAATTATTg                                                                                                                         >  2:926730/1‑140 (MQ=255)           cGAATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGTCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTTAA‑TTCGTAAAACCCCTGGCTTCGGCCCACGTTCGGAACTGAAATTATTg                                                                                                                         <  1:855082/140‑1 (MQ=255)            gAATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGTCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTTAA‑TTCGTAAAACCCCTGGCTTCGGCCCACGTTCGGAACTGAAATTATTGc                                                                                                                        <  1:500230/140‑1 (MQ=255)            gAATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGTCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTTAA‑TTCGTAAAACCCCTGGCTTCGGCCCACGTTCGGAACTGAAATTATTGc                                                                                                                        <  2:53714/140‑1 (MQ=255)            gAATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGTCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTTAA‑TTCGTAAAACCCCTGGCTTCGGCCCACGTTCGGAACTGAAATTATTGc                                                                                                                        <  1:1420371/140‑1 (MQ=255)                  ttATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGTCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTTAA‑TTCGTAAAACCCCTGGCTTCGGCCCACGTTCGGAACTGAAATTATTGCAGGATg                                                                                                                  <  2:780876/140‑1 (MQ=255)                    aTGTCCGTACCTTCTTCCGTGGCGTTGATATTAGTCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTTAA‑TTCGTAAAACCCCTGGCTTCGGCCCACGTTCGGAACTGAAATTATTg                                                                                                                         <  1:1010834/131‑1 (MQ=255)                    aTGTCCGTACCTTCTTCCGTGGCGTTGATATTAGTCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTTAA‑TTCGTAAAACCCCTGGCTTCGGCCCACGTTCGGAACTGAAATTATTg                                                                                                                         >  2:1010834/1‑131 (MQ=255)                       tCCGTACCTTCTTCCGTGGCGTTGATATTAGTCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTTAA‑TTCGTAAAACCCCTGGCTTCGGCCCACGTTCGTAACTGAAATTATTGCAGGATGAAGAt                                                                                                             >  2:548048/1‑140 (MQ=255)                         cGTACCTTCTTCCGTGGCGTTGATATTAGTCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTTAA‑TTCGTAAAACCCCTGGCTTCGGCCCACGTTCGGAACTGAAATTATTGCAGGATGAAGATcg                                                                                                           >  1:637686/1‑140 (MQ=255)                          gTACCTTCTTCCGTGGCGTTGATATTAGTCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTTAA‑TTCGTAAAACCCCTGGCTTCGGCCCACGTTCGGAACTGAAATTATTGCAGGATGAAGATcgc                                                                                                          >  2:1249776/1‑140 (MQ=255)                          gTACCTTCTTCCGTGGCGTTGATATTAGTCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTTAA‑TTCGTAAAACCCCTGGCTTCGGCCCACGTTCGGAACTGAAATTATTGCAGGATGAAGATcgc                                                                                                          >  1:610705/1‑140 (MQ=255)                           tACCTTCTTCCGTGGCGTTGATATTAGTCAGGTATTCGGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTTAA‑TTCGTAAAACCCCTGGCTTCGGCCCACGTTCGGAACTGAAATTATTGCAGGATGAAGATcgcg                                                                                                         >  2:1436020/1‑140 (MQ=255)                           tACCTTCTTCCGTGGCGTTGATATTAGTCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTTAA‑TTCGTAAAACCCCTGGCTTCGGCCCACGTTCGGAACTGAAATTATTGc                                                                                                                        <  2:494497/125‑1 (MQ=255)                           tACCTTCTTCCGTGGCGTTGATATTAGTCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTTAA‑TTCGTAAAACCCCTGGCTTCGGCCCACGTTCGGAACTGAAATTATTGc                                                                                                                        >  1:494497/1‑125 (MQ=255)                           tACCTTCTTCCGTGGCGTTGATATTAGTCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTTAA‑TTCGTAAAACCCCTGGCTTCGGCCCACGTTCGGAACTGAAATTATTGCAGGATGAAGATcgcg                                                                                                         >  2:1192130/1‑140 (MQ=255)                           tACCTTCTTCCGTGGCGTTGATATTAGTCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTTAA‑TTCGTAAAACCCCTGGCTTCGGCCCACGTTCGGAACTGAAATTATTGCAGGATGAAGATcgcg                                                                                                         >  2:1294417/1‑140 (MQ=255)                           tACCTTCTTCCGTGGCGTTGATATTAGTCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTTAA‑TTCGTAAAACCCCTGGCTTCGGCCCACGTTCGGAACTGAAATTATTGCAGGATGAAGATcgcg                                                                                                         >  2:662232/1‑140 (MQ=255)                           tACCTTCTTCCGTGGCGTTGATATTAGTCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTTAA‑TTCGTAAAACCCCTGGCTTCGGCCCACGTTCGGAACTGAAATTATTGCAGGATGAAGATcgcg                                                                                                         >  2:902055/1‑140 (MQ=255)                            aCCTTCTTCCGTGGCGTTGATATTAGTCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTTAA‑TTCGTAAAACCCCTGGCTTCGGCCCACGTTCGGAACTGAAATTATTGCAGGATGAAGATcgc                                                                                                          <  2:1100625/138‑1 (MQ=255)                            aCCTTCTTCCGTGGCGTTGATATTAGTCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTTAA‑TTCGTAAAACCCCTGGCTTCGGCCCACGTTCGGAACTGAAATTATTGCAGGATGAAGATcgc                                                                                                          >  1:1100625/1‑138 (MQ=255)                             ccTTCTTCCGTGGCGTTGATATTAGTCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTTAA‑TTCGTAAAACCCCTGGCTTCGGCCCACGTTCGTAACTGAAATTATTGCAGGATGAAGATCGCGaa                                                                                                       <  1:548048/140‑1 (MQ=255)                              cttcttCCGTGGCGTTGATATTAGTCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTTAA‑TTCGTAAAACCCCTGGCTTCGGCCCACGTTCGGAACTGAAATTATTGCAGGATGAAGATCGCGAat                                                                                                      <  2:610705/140‑1 (MQ=255)                                tcttcCGTGGCGTTGATATTAGTCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTTAA‑TTCGTAAAACCCCTGGCTTCGGCCCACGTTCGGAACTGAAATTATTGCAGGATGAAGATCGCGAatat                                                                                                    >  2:132948/1‑140 (MQ=255)                                 cttcCGTGGCGTTGATATTAGTCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTTAA‑TTCGTAAAACCCCTGGCTTCGGCCCACGTTCGGAACTGAAATTATTGCAGGATGAAGATCGCGAATATg                                                                                                   >  2:1283576/1‑140 (MQ=255)                                  ttcCGTGGCGTTGATATTAGTCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTTAA‑TTCGTAAAACCCCTGGCTTCGGCCCACGTTCGGAACTGAAATTATTGCAGGATGAAGATCGCGAATATgg                                                                                                  <  2:206044/140‑1 (MQ=255)                                  ttcCGTGGCGTTGATATTAGTCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTTAA‑TTCGTAAAACCCCTGGCTTCGGCCCACGTTCGGAACTGAAATTATTGCAGGATGAAGATCGCGAATATgg                                                                                                  >  1:668918/1‑140 (MQ=255)                                    ccGTGGCGTTGATATTAGTCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTTAA‑TTCGTAAAACCCCTGGCTTCGGCCCACGTTCGGAACTGAAATTATTGCAGGATGAAGATCGCGAATATGGCt                                                                                                <  1:902055/140‑1 (MQ=255)                                       tGGCGTTGATATTAGTCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTTAA‑TTCGTAAAACCCCTGGCTTCGGCCCACGTTCGGAACTGAAATTATTGCAGGATGAAGATCGCGAATATGGCTAcc                                                                                             >  2:236871/1‑139 (MQ=255)                                       tGGCGTTGATATTAGTCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTTAA‑TTCGTAAAACCCCTGGCTTCGGCCCACGTTCGGAACTGAAATTATTGCAGGATGAAGATCGCGAATATGGCTACg                                                                                             <  1:1423483/140‑1 (MQ=255)                                       tGGCGTTGATATTAGTCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTTAA‑TTCGTAAAACCCCTGGCTTCGGCCCACGTTCGGAACTGAAATTATTGCAGGATGAAGATCGCGAATATGGCTACg                                                                                             >  1:752522/1‑140 (MQ=255)                                       tGGCGTTGATATTAGTCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTTAA‑TTCGTAAAACCCCTGGCTTCGGCCCACGTTCGGAACTGAAATTATTGCAGGATGAAGATCGCGAATATGGCTACg                                                                                             >  1:511393/1‑140 (MQ=255)                                       tGGCGTTGATATTAGTCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTTAA‑TTCGTAAAACCCCTGGCTTCGGCCCACGTTCGGAACTGAAATTATTGCAGGATGAAGATCGCGAATATGGCTACg                                                                                             >  2:942286/1‑140 (MQ=255)                                          cGTTGATATTAGTCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTTAA‑TTCGTAAAACCCCTGGCTTCGGCCCACGTTCGGAACTGAAATTATTGCAGGATGAAGATCGCGAATATGGCTACGTTa                                                                                          <  2:532837/140‑1 (MQ=255)                                            ttGATATTAGTCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTTAA‑TTCGTAAAACCCCTGGCTTCGGCCCACGTTCGGAACTGAAATTATTGCAGGATGAAGATCGCGAATATGGCTACGTTATc                                                                                        >  2:492815/1‑140 (MQ=255)                                               atatTAGTCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTTAA‑TTCGTAAAACCCCTGGCTTCGGCCCACGTTCGGAACTGAAATTATTGCAGGATGAAGATCGCGAATATGGCTACGTTATCGaa                                                                                     <  2:637686/140‑1 (MQ=255)                                               atatTAGTCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTTAA‑TTCGTAAAACCCCTGGCTTCGGCCCACGTTCGGAACTG‑AATTATTGCAGGATGAAGATCGCGAATATGGCTACGTTATCGAAc                                                                                    <  2:197543/140‑1 (MQ=255)                                                tatTAGTCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTTAA‑TTCGTAAAACCCCTGGCTTCGGCCCACGTTCGGAACTGAAATTATTGCAGGATGAAGATCGCGAATATGGCTACGTTATCGAAc                                                                                    <  1:132948/140‑1 (MQ=255)                                                tatTAGTCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTTAA‑TTCGTAAAACCCCTGGCTTCGGCCCACGTTCGGAACTGAAATTATTGCAGGATGAAGATCGCGAATATGGCTACGTTATCGAAc                                                                                    <  1:1283576/140‑1 (MQ=255)                                                tatTAGTCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTTAA‑TTCGTAAAACCCCTGGCTTCGGCCCACGTTCGGAACTGAAATTATTGCAGGATGAAGATCGCGAATATGGCTACGTTATCGAAc                                                                                    <  1:36967/140‑1 (MQ=255)                                                tatTAGTCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTTAA‑TTCGTAAAACCCCTGGCTTCGGCCCACGTTCGGAACTGAAATTATTGCAGGATGAAGATCGCGAATATGGCTACGTTATCGAAc                                                                                    <  1:1249776/140‑1 (MQ=255)                                                 atTAGTCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTTAA‑TTCGTAAAACCCCTGGCTTCGGCCCACGTTCGGAACTGAAATTATTGCAGGATGAAGATCGCGAATATGGCTACGTTATCGAAcg                                                                                   <  2:668918/140‑1 (MQ=255)                                                 atTAGTCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTTAA‑TTCGTAAAACCCCTGGCTTCGGCCCACGTTCGGAACTGAAATTATTGCAGGATGAAGATCGCGAATATGGCTACGTTATCGAAcg                                                                                   <  1:35818/140‑1 (MQ=255)                                                 atTAGTCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTTAA‑TTCGTAAAACCCCTGGCTTCGGCCCACGTTCGGAACTGAAATTATTGCAGGATGAAGATCGCGAATATGGCTACGTTATCGAAcg                                                                                   <  2:809853/140‑1 (MQ=255)                                                 atTAGTCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTTAA‑TTCGTAAAACCCCTGGCTTCGGCCCACGTTCGGAACTGAAATTATTGCAGGATGAAGATCGCGAATATGGCTACGTTATCGAAcg                                                                                   <  1:67381/140‑1 (MQ=255)                                                 atTAGTCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTTAA‑TTCGTAAAACCCCTGGCTTCGGCCCACGTTCGGAACTGAAATTATTGCAGGATGAAGATCGCGAATATGGCTACGTTATCGAAcg                                                                                   <  1:1192130/140‑1 (MQ=255)                                                    aGTCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTTAA‑TTCGTAAAACCCCTGGCTTCGGCCCACGTTCGGAACTGAAATTATTGCAGGATGAAGATCGCGAATATGGCTACGTTATCGAACGCgg                                                                                <  2:752522/140‑1 (MQ=255)                                                      tCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTTAATTTCGTAAAACCCCTGGCTTCGGCCCACGTTCGGAACTGAAATTATTGCAGGATGAAGATCGCGAATATGGCTACGTTATCGAACGCGGt                                                                               >  1:744906/1‑140 (MQ=255)                                                         ggTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTTAA‑TTCGTAAAACCCCTGGCTTCGGCCCACGTTCGGAACTGAAATTATTGCAGGATGAAGATCGCGAATATGGCTACGTTATCGAAc                                                                                    >  2:1284500/1‑131 (MQ=255)                                                         ggTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTTAA‑TTCGTAAAACCCCTGGCTTCGGCCCACGTTCGGAACTGAAATTATTGCAGGATGAAGATCGCGAATATGGCTACGTTATCGAAc                                                                                    <  1:1284500/131‑1 (MQ=255)                                                         ggTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTTAA‑TTCGTAAAACCCCTGGCTTCGGCCCACGTTCGGAACTGAAATTATTGCAGGATGAAGATCGCGAATATGGCTACGTTATCGAACGCGGtaata                                                                           >  2:122001/1‑140 (MQ=255)                                                          gTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTTAA‑TTCGTAAAACCCCTGGCTTCGGCCCACGTTCGGAACTGAAATTATTGCAGGATGAAGATCGCGAATATGGCTGCGTTATCGaa                                                                                     >  1:1342875/1‑129 (MQ=255)                                                          gTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTTAA‑TTCGTAAAACCCCTGGCTTCGGCCCACGTTCGGAACTGAAATTATTGCAGGATGAAGATCGCGAATATGGCTACGTTATCGaa                                                                                     <  2:1342875/129‑1 (MQ=255)                                                          gTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTTAA‑TTCGTAAAACCCCTGGCTTCGGCCCACGTTCGGAACTGAAATTATTGCAGGATGAAGATCGCGAATATGGCTACGTTATCGAAcg                                                                                   <  2:689550/131‑1 (MQ=255)                                                          gTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTTAA‑TTCGTAAAACCCCTGGCTTCGGCCCACGTTCGGAACTGAAATTATTGCAGGATGAAGATCGCGAATATGGCTACGTTATCGAAcg                                                                                   >  1:689550/1‑131 (MQ=255)                                                          gTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTTAA‑TTCGTAAAACCCCTGGCTTCGGCCCACGTTCGGAACTGAAATTATTGCAGGATGAAGATCGCGAATATGGCTACGTTATCGAACGCGGtaataa                                                                          >  1:1277928/1‑140 (MQ=255)                                                                aGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTTAA‑TTCGTAAAACCCCTGGCTTCGGCCCACGTTCGGAACTGAAATTATTGCAGGATGAAGATCGCGAATATGGCTACGTTATCGaa                                                                                     >  1:600028/1‑123 (MQ=255)                                                                aGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTTAA‑TTCGTAAAACCCCTGGCTTCGGCCCACGTTCGGAACTGAAATTATTGCAGGATGAAGATCGCGAATATGGCTACGTTATCGaa                                                                                     <  2:600028/123‑1 (MQ=255)                                                                    cGAAAGATATTGCCCGCCGGACACCGAATGGCTTAA‑TTCGTAAAACCCCTGGTTTCGGCCCACGTTCGGAACTGAAATTATTGCAGGATGAAGATCGCGAATATGGCTACGTTATCGAACGCGGTAATAAGTTTGTCGgc                                                                >  1:1273397/1‑140 (MQ=255)                                                                    cGAAAGATATTGCCCGCCGGACACCGAATGGCTTAA‑TTCGTAAAACCCCTGGCTTCGGCCCACGTTCGGAACTGAAATTATTGCAGGATGAAGATCGCGAATATGGCTACGTTATCGAACGCGGTAATAAGTTTGTCGgc                                                                >  2:571305/1‑140 (MQ=255)                                                                     gAAAGATATTGCCCGCCGGACACCGAATGGCTTAA‑TTCGTAAAACCCCTGGCTTCGGCCCACGTTCGGAACTGAAATTATTGCAGGATGAAGATCGCGAATATGGCTACGTTATCGAACGCGGTAATAAGTTTGTCGgcg                                                               <  2:1277928/140‑1 (MQ=255)                                                                       aaGATATTGCCCGCCGGACACCGAATGGCTTAA‑TTCGTAAAACCCCTGGCTTCGGCCCACGTTCGGAACTGAAATTATTGCAGGATGAAGATCGCGAATATGGCTACGTTATCGAACGCGGTAATAAGTTTGTCGGCGCa                                                             <  1:545580/140‑1 (MQ=255)                                                                            atTGCCCGCCGGACACCGAATGGCTTAA‑TTCGTAAAACCCCTGGCTTCGGCCCACGTTCGGAACTGAAATTATTGCAGGATGAAGATCGCGAATATGGCTACGTTATCGAACGCGGTAATAAGTTTGTCGGCGCAGtctc                                                        >  1:120720/1‑140 (MQ=255)                                                                            atTGCCCGCCGGACACCGAATGGCTTAA‑TTCGTAAAACCCCTGGCTTCGGCCCACGTTCGGAACTGAAATTATTGCAGGATGAAGATCGCGAATATGGCTACGTTATCGAACGCGGTAATAAGTTTGTCGGCGCAGtctc                                                        >  1:227699/1‑140 (MQ=255)                                                                              tGCCCGCCGGACACCGAATGGCTTAA‑TTCGTAAAACCCCTGGCTTCGGCCCACGTTCGGAACTGAAATTATTGCAGGATGAAGATCGCGAATATGGCTACGTTATCGAACGCGGTAATAAGTTTGt                                                                    <  2:1263842/126‑1 (MQ=255)                                                                              tGCCCGCCGGACACCGAATGGCTTAA‑TTCGTAAAACCCCTGGCTTCGGCCCACGTTCGGAACTGAAATTATTGCAGGATGAAGATCGCGAATATGGCTACGTTATCGAACGCGGTAATAAGTTTGt                                                                    >  1:1263842/1‑126 (MQ=255)                                                                                   gccgGACACCGAATGGCTTAA‑TTCGTAAAACCCCTGGCTTCGGCCCACGTTCGGAACTGAAATTATTGCAGGATGAAGATCGCGAATATGGCTACGTTATCGAACGCGGTAATAAGTTTGTCGGCGCAGTCTCCATCGAt                                                 <  1:942286/140‑1 (MQ=255)                                                                                    ccgGACACCGAATGGCTTAATTTCGTAAAACCCCTGGCTTCGGCCCACGTTCGGAACTGAAATTATTGCAGGATGAAGATCGCGAATATGGCTACGTTATCGAACGCGGTAATAAGTTTGTCGGCGCAGTCTCCATCGAt                                                 <  2:744906/140‑1 (MQ=255)                                                                                    ccgGACACCGAATGGCTTAA‑TTCGTAAAACCCCTGGCTTCGGCCCACGTTCGGAACTGAAATTATTGCAGGATGAAGATCGCGAATATGGCTACGTTATCGAACGCGGTAATAAGTTTGTCGGCGCAGt                                                           <  2:325470/129‑1 (MQ=255)                                                                                    ccgGACACCGAATGGCTTAA‑TTCGTAAAACCCCTGGCTTCGGCCCACGTTCGGAACTGAAATTATTGCAGGATGAAGATCGCGAATATGGCTACGTTATCGAACGCGGTAATAAGTTTGTCGGCGCAGt                                                           >  1:325470/1‑129 (MQ=255)                                                                                      ggACACCGAATGGCTTAA‑TTCGTAAAACCCCTGGCTTCGGCCCACGTTCGGAACTGAAATTATTGCAGGATGAAGATCGCGAATATGGCTACGTTATCGAACGCGGTAATAAGTTTGTCGGCGCAGTCTCCATCGATTCg                                              >  1:1213432/1‑140 (MQ=255)                                                                                            cGAATGGCTTAA‑TTCGTAAAACCCCTGGCTTCGGCCCACGTTCGGAACTGAAATTATTGCAGGATGAAGATCGCGAATATGGCTACGTTATCGAACGCGGTAATAAGTTTGTCGGCGCAGTCTCCATCGATTCGCTTaaa                                        <  1:283816/140‑1 (MQ=255)                                                                                                      aa‑TTCGTAAAACCCCTGGCTTCGGCCCACGTTCGGAACTGAAATTATTGCAGGATGAAGATCGCGAATATGGCTACGTTATCGAACGCGGTAATAAGTTTGTCGGCGCAGTCTCCATCGATTCGCTTAAAACCGCGTTaa                              <  2:1271464/140‑1 (MQ=255)                                                                                                         ttCGTAAAACCCCTGGCTTCGGCCCACGTTCGGAACTGAAATTATTGCAGGATGAAGATCGCGAATATGGCTACGTTATCGAACGCGGTAATAAGTTTGTCGGCGCAGTCTCCATCGATTCGCTTAAAACCGCGTTAACg                            >  2:511648/1‑140 (MQ=255)                                                                                                             tAAAACCCCTGGCTTCGGCCCACGTTCGGAACTGAAATTATTGCAGGATGAAGATCGCGAATATGGCTACGTTATCGAACGCGGTAATAAGTTTGTCGGCGCAGTCTCCATCGATTCGCTTAAAACCGCGTTAACgcagc                        >  1:585100/1‑140 (MQ=255)                                                                                                             tAAAACCCCTGGCTTCGGCCCACGTTCGGAACTGAAATTATTGCAGGATGAAGATCGCGAATATGGCTACGTTATCGAACGCGGTAATAAGTTTGTCGGCGCAGTCTCCATCGATTCGCTTAAAACCGCGTTAACgcagc                        >  2:429750/1‑140 (MQ=255)                                                                                                                 aCCCCTGGCTTCGGCCCACGTTCGGAACTGAAATTATTGCAGGATGAAGATCGCGAATATGGCTACGTTATCGAACGCGGTAATAAGTTTGTCGGCGCAGTCTCCATCGATTCGCTTAAAACCGCGTTAACgcagcagca                    <  2:209493/140‑1 (MQ=255)                                                                                                                  ccccTGGCTTCGGCCCACGTTCGGAACTGAAATTATTGCAGGATGAAGATCGCGAATATGGCTACGTTATCGAACGCGGTAATAAGTTTGTCGGCGCAGTCTCCATCGATTCGCTTAAAACCGCGTTAACGCAGCAGCaa                   <  2:1025878/140‑1 (MQ=255)                                                                                                                  ccccTGGCTTCGGCCCACGTTCGGAACTGAAATTATTGCAGGATGAAGATCGCGAATATGGCTACGTTATCGAACGCGGTAATAAGTTTGTCGGCGCAGTCTCCATCGATTCGCTTAAAACCGCGTTAACGCAGCAGCaa                   >  2:1405/1‑140 (MQ=255)                                                                                                                      tGGCTTCGGCCCACGTTCGGAACTGAAATTATTGCAGGATGAAGATCGCGAATATGGCTACGTTATCGAACGCGGTAATAAGTTTGTCGGCGCAGTCTCCATCGATTCGCTTAAAACCGCGTTAACGCAGCAGCAAGGTc               >  1:1406354/1‑140 (MQ=255)                                                                                                                            cGGCCCACGTTCGGAACTGAAATTATTGCAGGATGAAGATCGCGAATATGGCTACGTTATCGAACGCGGTAATAAGTTTGTCGGCGCAGTCTCCATCGATTCGCTTAAAACCGCGTTAACGCAGCAGCAAGGTCTTGATg         <  2:120720/140‑1 (MQ=255)                                                                                                                             ggCCCACGTTCGGAACTGAAATTATTGCAGGATGAAGATCGCGAATATGGCTACGTTATCGAACGCGGTAATAAGTTTGTCGGCGCAGTCTCCATCGATTCGCTTAAAACCGCGTTAACGCAGCAGCAAGGTCTTGATgc        >  1:865387/1‑140 (MQ=255)                                                                                                                             ggCCCACGTTCGGAACTGAAATTATTGCAGGATGAAGATCGCGAATATGGCTACGTTATCGAACGCGGTAATAAGTTTGTCGGCGCAGTCTCCATCGATTCGCTTAAAACCGCGTTAACGCAGCAGCAAGGTCTTGATgc        >  1:1292145/1‑140 (MQ=255)                                                                                                                             ggCCCACGTTCGGAACTGAAATTATTGCAGGATGAAGATCGCGAATATGGCTACGTTATCGAACGCGGTAATAAGTTTGTCGGCGCAGTCTCCATCGATTCGCTTAAAACCGCGTTAACGCAGCAGCAAGGTCTTGATgc        >  2:328164/1‑140 (MQ=255)                                                                                                                              gCCCACGTTCGGAACTGAAATTATTGCAGGATGAAGATCGCGAATATGGCTACGTTATCGAACGCGGTAATAAGTTTGTCGGCGCAGTCTCCATCGATTCGCTTAAAACCGCGTTAACGCAGCAGCAAGGTCTTGATgcg       <  2:865387/140‑1 (MQ=255)                                                                                                                                 cACGTTCGGAACTGAAATTATTGCAGGATGAAGATCGCGAATATGGCTACGTTATCGAACGCGGTAATAAGTTTGTCGGCGCAGTCTCCATCGATTCGCTTAAAACCGCGTTAACGCAGCAGCAAGGTCTTGATgcggcg    <  2:1292145/140‑1 (MQ=255)                                                                                                                                  aCGTTCGGAACTGAAATTATTGCAGGATGAAGATCGCGAATATGGCTACGTTATCGAACGCGGTAATAAGTTTGTCGGCGCAGTCTCCATCGATTCGCTTAAAACCGCGTTAACGCAGCAGCAAGGTCTTGATGCGgcgc   >  1:441023/1‑140 (MQ=255)                                                                                                                                   cGTTCGGAACTGAAATTATTGCAGGATGAAGATCGCGAATATGGCTACGTTATCGAACGCGGTAATAAGTTTGTCGGCGCAGTCTCCATCGATTCGCTTAAAACCGCGTTAACGCAGCAGCAAGGTCTTGATGCGGCGCt  >  2:1078863/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                     AATAATCCGGCGAATGATTATGTCCGTACCTTCTTCCGTGGCGTTGATATTAGTCAGGTATTCAGTGCGAAAGATATTGCCCGCCGGACACCGAATGGCTTAA‑TTCGTAAAACCCCTGGCTTCGGCCCACGTTCGGCACTGAAATTATTGCAGGATGAAGATCGCGAATATGGCTACGTTATCGAACGCGGTAATAAGTTTGTCGGCGCAGTCTCCATCGATTCGCTTAAAACCGCGTTAACGCAGCAGCAAGGTCTTGATGCGGCGCT  >  NC_000913/2805574‑2805842

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑