Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,618,914 G→T Q109H (CAG→CAT)  nikR → DNA‑binding transcriptional repressor NikR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,618,9140GT99.3% 498.4 / ‑3.3 142Q109H (CAG→CAT) nikRDNA‑binding transcriptional repressor NikR
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base T (69/72);  total (69/73)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.45e-01

Only 100 of 145 total aligned reads displayed.

ACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCCGTCGCCACGCTGCATGTGCACATCAACCACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGTGACGTGCAGCATTTTGCCGATGACGTTATCGCCCAGCGCGGCGTGCGGCACGGGCATTTGCAGTGCTTGCCGAAGGAAGATTGAGTCTATTATTGGGTCCTTTGGTGAACGATCAACAAAGGGCCACTAGCACACCTGATTCCTC  >  NC_000913/3618779‑3619050
                                                                                                                                       |                                                                                                                                        
aCGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCCGTCGCCACGCTGCATGTGCACATCAACCACGACGACTGTCTGGAAATCGCGGTGTTGAAAGGTGACATGGGTGACGTGCATCAtt                                                                                                                                      <  1:466889/140‑1 (MQ=255)
  gcgACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCCGTCGCCACGCTGCATGTGCACATCAACCACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGTGACGTGCATCAtttt                                                                                                                                    >  1:1401840/1‑140 (MQ=255)
       ttAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGTCCTCTCCGTCGCCACGCTGCATGTGCACATCAACCACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGTGACGTGCATCATTTTGCCGa                                                                                                                               <  1:136096/140‑1 (MQ=255)
          gccagccGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCCGTCGCCACGCTGCATGTGCACATCAACCACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGTGACGTGCATCATTTTGCCGATGa                                                                                                                            <  2:636616/140‑1 (MQ=255)
               ccGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCCGTCGCCACGCTGCATGTGCACATCAACCACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGTGACGTGCATCATTTTGCCGATGACGTTa                                                                                                                       >  1:599243/1‑140 (MQ=255)
                cGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCCGTCGCCACGCTGCATGTGCACATCAACCACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGTGACGTGCATCAttt                                                                                                                                     >  1:1219559/1‑125 (MQ=255)
                cGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCCGTCGCCACGCTGCATGTGCACATCAACCACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGTGACGTGCATCATTTTGCCGATGACGTTAt                                                                                                                      >  1:728097/1‑140 (MQ=255)
                 gCATTGTCTCCACCCAGCATCATCACCACGACCTCTCCGTCGCCACGCTGCATGTGCACATCAACCACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGTGACGTGCATCATTTTGCCGATGACGTTa                                                                                                                       >  2:1275760/1‑138 (MQ=255)
                 gCATTGTCTCCACCCAGCATCATCACCACGACCTCTCCGTCGCCACGCTGCATGTGCACATCAACCACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGTGACGTGCATCATTTTGCCGATGACGTTa                                                                                                                       <  1:1275760/138‑1 (MQ=255)
                 gCATTGTCTCCACCCAGCATCATCACCACGACCTCTCCGTCGCCACGCTGCATGTGCACATCAACCACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGTGACGTGCATCATTTTGCCGATGACGTTATc                                                                                                                     >  2:553282/1‑140 (MQ=255)
                    ttGTCTCCACCCAGCATCATCACCACGACCTCTCCGTCGCCACGCTGCATGTGCACATCAACCACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGTGACGTGCATCATTTTGCCGATGACGTTATCGcc                                                                                                                  >  1:954342/1‑140 (MQ=255)
                           cACCCAGCATCATCACCACGACCTCTCCGTCGCCACGCTGCATGTGCACATCAACCACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGTGACGTGCATCATTTTGCCGATGACGTTATCGCCCAGcgcg                                                                                                           <  1:229474/140‑1 (MQ=255)
                             cccAGCATCATCACCACGACCTCTCCGTCGCCACGCTGCATGTGCACATCAACCACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGTGACGTGCATCATTTTGCCGATGACGTTATCGCCCAGCgcggc                                                                                                         >  1:167060/1‑140 (MQ=255)
                                 gCATCATCACCACGACCTCTCCGTCGCCACGCTGCATGTGCACATCAACCACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGTGACGTGCATCATTTTGCCGATGACGTTATCGcc                                                                                                                  <  1:407998/127‑1 (MQ=255)
                                 gCATCATCACCACGACCTCTCCGTCGCCACGCTGCATGTGCACATCAACCACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGTGACGTGCATCATTTTGCCGATGACGTTATCGcc                                                                                                                  >  2:407998/1‑127 (MQ=255)
                                 gCATCATCACCACGACCTCTCCGTCGCCACGCTGCATGTGCACATCAACCACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGTGACGTGCATCATTTTGCCGATGACGTTATCGCCCAGCGCGGCGTGc                                                                                                     >  2:472449/1‑140 (MQ=255)
                                 gCATCATCACCACGACCTCTCCGTCGCCACGCTGCATGTGCACATCAACCACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGTGACGTGCATCATTTTGCCGATGACGTTATCGCCCAGCGCGGCGTGc                                                                                                     >  1:1336338/1‑140 (MQ=255)
                                 gCATCATCACCACGACCTCTCCGTCGCCACGCTGCATGTGCACATCAACCACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGTGACGTGCATCATTTTGCCGATGACGTTATCGCCCAGCGCGGCGTGc                                                                                                     >  2:839145/1‑140 (MQ=255)
                                  catcatCACCACGACCTCTCCGTCGCCACGCTGCATGTGCACATCAACCACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGTGACGTGCATCATTTTGCCGATGACGTTATCGCCCAGCGCGGCGTGCg                                                                                                    >  2:297777/1‑140 (MQ=255)
                                  catcatCACCACGACCTCTCCGTCGCCACGCTGCATGTGCACATCAACCACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGTGACGTGCATCATTTTGCCGATGACGTTATCGCCCAGCGCGGCGTGCg                                                                                                    >  1:1244964/1‑140 (MQ=255)
                                  catcatCACCACGACCTCTCCGTCGCCACGCTGCATGTGCACATCAACCACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGTGACGTGCATCATTTTGCCGATGACGTTATCGCCCAGCGCGGCGTGCg                                                                                                    >  2:710542/1‑140 (MQ=255)
                                       tcaCCACGACCTCTCCGTCGCCACGCTGCATGTGCACATCAACCACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGTGACGTGCATCATTTTGCCGATGACGTTATCGCCCAGCGCGGCGTg                                                                                                      <  2:452352/133‑1 (MQ=255)
                                       tcaCCACGACCTCTCCGTCGCCACGCTGCATGTGCACATCAACCACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGTGACGTGCATCATTTTGCCGATGACGTTATCGCCCAGCGCGGCGTGCGGCACg                                                                                               >  1:927169/1‑140 (MQ=255)
                                          ccacGACCTCTCCGTCGCCACGCTGCATGTGCACATCAACCACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGTGACGTGCATCATTTTGCCGATGACGTTATCGCCCAGCGCGGCGTGCGGCACGGGc                                                                                            <  1:1271407/140‑1 (MQ=255)
                                          ccacGACCTCTCCGTCGCCACGCTGCATGTGCACATCAACCACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGTGACGTGCATCATTTTGCCGATGACGTTATCGCCCAGCGCGGCGTGCGGCACGGGc                                                                                            <  2:511934/140‑1 (MQ=255)
                                             cGACCTCTCCGTCGCCACGCTGCATGTGCACATCAACCACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGTGACGTGCATCATTTTGCCGATGACGTTATCGCCCAGCGCGGCGTGCGGCACGGGCAtt                                                                                         >  1:658280/1‑140 (MQ=255)
                                                   ctcCGTCGCCACGCTGCATGTGCACATCAACCACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGTGACGTGCATCATTTTGCCGATGACGTTATCGCCCAGCGCGGCGTGCGGCACGGGCATTTGCAGt                                                                                   >  2:1211727/1‑140 (MQ=255)
                                                   ctcCGTCGCCACGCTGCATGTGCACATCAACCACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGTGACGTGCATCATTTTGCCGATGACGTTATCGCCCAGCGCGGCGTGCGGCACGGGCATTTGCAGt                                                                                   >  2:1158952/1‑140 (MQ=255)
                                                     ccGTCGCCACGCTGCATGTGCACATCAACCACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGTGACGTGCATCATTTTGCCGATGACGTTATCGCCCAGCGCGGCGTGCGGCACGGGCATTTGCAGTGc                                                                                 >  1:1025126/1‑140 (MQ=255)
                                                      cGTCGCCACGCTGCATGTGCACATCAACCACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGTGACGTGCATCATTTTGCCGATGACGTTATCGCCCAGCGCGGCGTGCGGCACGGGCATTTGCAGTGCt                                                                                >  1:198615/1‑140 (MQ=255)
                                                         cGCCACGCTGCATGTGCACATCAACCACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGTGACGTGCATCATTTTGCCGATGACGTTATCGCCCAGCGCGGCGTGCGGCACGGGCATTTGCAGTGCTTGc                                                                             <  2:925921/140‑1 (MQ=255)
                                                         cGCCACGCTGCATGTGCACATCAACCACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGTGACGTGCATCATTTTGCCGATGACGTTATCGCCCAGCGCGGCGTGCGGCACGGGCATTTGCAGTGCTTGc                                                                             <  1:710542/140‑1 (MQ=255)
                                                          gCCACGCTGCATGTGCACATCAACCACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGTGACGTGCATCATTTTGCCGATGACGTTATCGCCCAGCGCGGCGTGCGGCACGGGCATTTGCAGTGCTTGcc                                                                            >  2:874724/1‑140 (MQ=255)
                                                           ccACGCTGCATGTGCACATCAACCACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGTGACGTGCATCATTTTGCCGATGACGTTATCGCCCAGCGCGGCGTGCGGCACGGGCATTTGCAGTGCTTGCCg                                                                           <  1:901282/140‑1 (MQ=255)
                                                           ccACGCTGCATGTGCACATCAACCACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGTGACGTGCATCATTTTGCCGATGACGTTATCGCCCAGCGCGGCGTGCGGCACGGGCATTTGCAGTGCTTGCCg                                                                           <  2:1226683/140‑1 (MQ=255)
                                                              cGCTGCATGTGCACATCAACCACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGTGACGTGCATCATTTTGCCGATGACGTTATCGCCCAGCGCGGCGTGCGGCACGGGCATTTGCAGTGCTTGCCgaag                                                                        >  2:107694/1‑140 (MQ=255)
                                                              cGCTGCATGTGCACATCAACCACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGTGACGTGCATCATTTTGCCGATGACGTTATCGCCCAGCGCGGCGTGCGGCACGGGCATTTGCAGTGCTTGCCgaag                                                                        >  2:362459/1‑140 (MQ=255)
                                                               gCTGCATGTGCACATCAACCACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGTGACGTGCATCATTTTGCCGATGACGTTATCGCCCAGCGCGGCGTGCGGCACGGGCATTTGCAGTGCTTGCCgaagg                                                                       <  2:559672/140‑1 (MQ=255)
                                                               gCTGCATGTGCACATCAACCACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGTGACGTGCATCATTTTGCCGATGACGTTATCGCCCAGCGCGGCGTGCGGCACGGGCATTTGCAGTGCTTGCCgaagg                                                                       <  2:659272/140‑1 (MQ=255)
                                                                cTGCATGTGCACATCAACCACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGTGACGTGCATCATTTTGCCGATGACGTTATCGCCCAGCGCGGCGTGCGGCACGGGCATTTGCAGTGCTTGCCgaagga                                                                      >  2:683239/1‑140 (MQ=255)
                                                                     tgtgCACATCAACCACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGTGACGTGCATCATTTTGCCGATGACGTTATCGCCCAGCGCGGCGTGCGGCACGGGCATTTGCAGTGCTTGCCGAAGGAAGAtt                                                                 <  2:599243/140‑1 (MQ=255)
                                                                     tgtgCACATCAACCACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGTGACGTGCATCATTTTGCCGATGACGTTATCGCCCAGCGCGGCGTGCGGCACGGGCATTTGCAGTGCTTGCCGAAGGAAGAtt                                                                 <  1:865264/140‑1 (MQ=255)
                                                                     tgtgCACATCAACCACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGTGACGTGCATCATTTTGCCGATGACGTTATCGCCCAGCGCGGCGTGCGGCACGGGCATTTGCAGTGCTTGCCGAAGGAAGAtt                                                                 <  1:839145/140‑1 (MQ=255)
                                                                     tgtgCACATCAACCACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGTGACGTGCATCATTTTGCCGATGACGTTATCGCCCAGCGCGGCGTGCGGCACGGGCATTTGCAGTGCTTGCCGAAGGAAGAtt                                                                 <  2:198615/140‑1 (MQ=255)
                                                                     tgtgCACATCAACCACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGTGACGTGCATCATTTTGCCGATGACGTTATCGCCCAGCGCGGCGTGCGGCACGGGCATTTGCAGTGCTTGCCGAAGGAAGAtt                                                                 <  1:553282/140‑1 (MQ=255)
                                                                     tgtgCACATCAACCACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGTGACGTGCATCATTTTGCCGATGACGTTATCGCCCAGCGCGGCGTGCGGCACGGGCATTTGCAGTGCTTGCCGAAGGAAGAtt                                                                 <  2:618088/140‑1 (MQ=255)
                                                                       tgCACATCAACCACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGTGACGTGCATCATTTTGCCGATGACGTTATCGCCCAGCGCGGCGTGCGGCACGGGCATTTGCAGTGCTTGCCGAAGGAAGATTGa                                                               <  1:1228019/140‑1 (MQ=255)
                                                                       tgCACATCAACCACGACAACTGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGTGACGTGCAGCATTTTGCCGATGACGTTATCGCCCAGCGCGGCGTGCGGCACGGGCATTTGCAGTGCTTGCCGAAGGAAGATTGa                                                               <  2:420217/140‑1 (MQ=255)
                                                                        gCACATCAACCACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGTGACGTGCATCATTTTGCCGATGACGTTATCGCCCAGCGCGGCGTGCGGCACGGGCATTTGCAGTGCTTGCCGAAGGAAGATTGAg                                                              >  1:1302550/1‑140 (MQ=255)
                                                                         cacaTCAACCACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGTGACGTGCATCATTTTGCCGATGACGTTATCGCCCAGCGCGGCGTGCGGCACGGGCATTTGCAGTGCTTGCCGAAGGAAGATTGAGt                                                             <  2:789969/140‑1 (MQ=255)
                                                                         cacaTCAACCACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGTGACGTGCATCATTTTGCCGATGACGTTATCGCCCAGCGCGGCGTGCGGCACGGGCATTTGCAGTGCTTGCCGAAGGAAGATTGAGt                                                             <  2:1359494/140‑1 (MQ=255)
                                                                         cacaTCAACCACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGTGACGTGCATCATTTTGCCGATGACGTTATCGCCCAGCGCGGCGTGCGGCACGGGCATTTGCAGTGCTTGCCGAAGGAAGATTGAGt                                                             <  2:984865/140‑1 (MQ=255)
                                                                         cacaTCAACCACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGTGACGTGCATCATTTTGCCGATGACGTTATCGCCCAGCGCGGCGTGCGGCACGGGCATTTGCAGTGCTTGCCGAAGGAAGATTGAGt                                                             >  1:660656/1‑140 (MQ=255)
                                                                           caTCAACCACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGTGACGTGCATCATTTTGCCGATGACGTTATCGCCCAGCGCGGCGTGCGGCACGGGCATTTGCAGTGCTTGCCGAAGGAAGATTGAGTCt                                                           >  1:993984/1‑140 (MQ=255)
                                                                           caTCAACCACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGTGACGTGCATCATTTTGCCGATGACGTTATCGCCCAGCGCGGCGTGCGGCACGGGCATTTGCAGTGCTTGCCGAAGGAAGATTGAGTCt                                                           >  2:1305988/1‑140 (MQ=255)
                                                                            aTCAACCACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGTGACGTGCATCATTTTGCCGATGACGTTATCGCCCAGCGCGGCGTGCGGCACGGGCATTTGCAGTGCTTGCCGAAGGAAGATTGAGTCta                                                          <  2:569405/140‑1 (MQ=255)
                                                                             tCAACCACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGTGACGTGCATCATTTTGCCGATGACGTTATCGCCCAGCGCGGCGTGCGGCACGGGCATTTGCAGTGCTTGCCGAAGGAAGAtt                                                                 <  2:1261609/132‑1 (MQ=255)
                                                                             tCAACAACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGTGACGTGCATCATTTTGCCGATGACGTTATCGCCCAGCGCGGCGTGCGGCACGGGCATTTGCAGTGCTTGCCGAAGGAAGATTGAGTCtat                                                         >  2:1090406/1‑140 (MQ=255)
                                                                              cAACCACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGTGACGTGCATCATTTTGCCGATGACGTTATCGCCCAGCGCGGCGTGCGGCACGGGCATTTGCAGTGCTTGCCGAAGGAAGATTGAGTCtatt                                                        >  1:552607/1‑140 (MQ=255)
                                                                               aaCCACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGTGACGTGCATCATTTTGCCGATGACGTTATCGCCCAGCGCGGCGTGCGGCACGGGCATTTGCAGTGCTTGCCGAAGGAAGAtt                                                                 >  1:423501/1‑130 (MQ=255)
                                                                                 ccACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGTGACGTGCATCATTTTGCCGATGACGTTATCGCCCAGCGCGGCGTGCGGCACGGGCATTTGCAGTGCTTGCCGAAGGAAGATTGAGTc                                                            <  1:207844/133‑1 (MQ=255)
                                                                                 ccACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGTGACGTGCATCATTTTGCCGATGACGTTATCGCCCAGCGCGGCGTGCGGCACGGGCATTTGCAGTGCTTGCCGAAGGAAGATTGAGTCta                                                          >  2:93861/1‑135 (MQ=255)
                                                                                 ccACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGTGACGTGCATCATTTTGCCGATGACGTTATCGCCCAGCGCGGCGTGCGGCACGGGCATTTGCAGTGCTTGCCGAAGGAAGATTGAGTCTattatt                                                     >  2:1286951/1‑140 (MQ=255)
                                                                                 ccACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGTGACGTGCATCATTTTGCCGATGACGTTATCGCCCAGCGCGGCGTGCGGCACGGGCATTTGCAGTGCTTGCCGAAGGAAGATTGAGTCTattatt                                                     >  1:303731/1‑140 (MQ=255)
                                                                                      acgacTGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGTGACGTGCATCATTTTGCCGATGACGTTATCGCCCAGCGCGGCGTGCGGCACGGGCATTTGCAGTGCTTGCCGAAGGAAGATTGAGTCTATTATTGGGTc                                                >  1:1358708/1‑140 (MQ=255)
                                                                                      acgacTGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGTGACGTGCATCATTTTGCCGATGACGTTATCGCCCAGCGCGGCGTGCGGCACGGGCATTTGCAGTGCTTGCCGAAGGAAGATTGAGTCTATTATTGGGTc                                                >  2:917227/1‑140 (MQ=255)
                                                                                          cTGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGTGACGTGCATCATTTTGCCGATGACGTTATCGCCCAGCGCGGCGTGCGGCACGGGCATTTGCAGTGCTTGCCGAAGGAAGATTGAGTCTATTATTGGGTCCttt                                            >  2:332971/1‑140 (MQ=255)
                                                                                            gTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGTGACGTGCATCATTTTGCCGATGACGTTATCGCCCAGCGCGGCGTGCGGCACGGGCATTTGCAGTGCTTGCCGAAGGAAGATTGAGTCTATTATTGGGTCCTTTgg                                          >  2:153197/1‑140 (MQ=255)
                                                                                             tCTGGAAATCGCCGTGTTGAAAGGAGACATGGGTGACGTGCATCATTTTGCCGATGACGTTATCGCCCAGCGCGGCGTGCGGCACGGGCATTTGCAGTGCTTGCCGAAGGAAGATTGAGTCTATTATTGGGTCCTTTGGt                                         >  1:518919/1‑140 (MQ=255)
                                                                                               tGGAAATCGCCGTGTTGAAAGGTGACATGGGTGACGTGCATCATTTTGCCGATGACGTTATCGCCCAGCGCGGCGTGCGGCACGGGCATTTGCAGTGCTTGCCGAAGGAAGATTGAGTCTATTATTGGGTCCTTTGGTGa                                       <  2:1302550/140‑1 (MQ=255)
                                                                                                ggAAATCGCCGTGTTGAAAGGTGACATGGGTGACGTGCATCATTTTGCCGATGACGTTATCGCCCAGCGCGGCGTGCGGCACGGGCATTTGCAGTGCTTGCCGAAGGAAGATTGAGTCTATTATTGGGTCCTTTGGTGaa                                      <  1:1002656/140‑1 (MQ=255)
                                                                                                ggAAATCGCCGTGTTGAAAGGTGACATGGGTGACGTGCATCATTTTGCCGATGACGTTATCGCCCAGCGCGGCGTGCGGCACGGGCATTTGCAGTGCTTGCCGAAGGAAGATTGAGTCTATTATTGGGTCCTTTGGTGaa                                      <  1:1305988/140‑1 (MQ=255)
                                                                                                ggAAATCGCCGTGTTGAAAGGTGACATGGGTGACGTGCATCATTTTGCCGATGACGTTATCGCCCAGCGCGGCGTGCGGCACGGGCATTTGCAGTGCTTGCCGAAGGAAGATTGAGTCTATTATTGGGTCCTTTGGTGaa                                      <  2:87460/140‑1 (MQ=255)
                                                                                                 gAAATCGCCGTGTTGAAAGGTGACATGGGTGACGTGCATCATTTTGCCGATGACGTTATCGCCCAGCGCGGCGTGCGGCACGGGCATTTGCAGTGCTTGCCGAAGGAAGATTGAGTCTATTATTGGGTCCTTTGGTGa                                       <  1:1211711/138‑1 (MQ=255)
                                                                                                 gAAATCGCCGTGTTGAAAGGTGACATGGGTGACGTGCATCATTTTGCCGATGACGTTATCGCCCAGCGCGGCGTGCGGCACGGGCATTTGCAGTGCTTGCCGAAGGAAGATTGAGTCTATTATTGGGTCCTTTGGTGa                                       >  2:1211711/1‑138 (MQ=255)
                                                                                                 gAAATCGCCGTGTTGAAAGGTGACATGGGTGACGTGCATCATTTTGCCGATGACGTTATCGCCCAGCGCGGCGTGCGGCACGGGCATTTGCAGTGCTTGCCGAAGGAAGATTGAGTCTATTATTGGGTCCTTTGGTGAAc                                     <  1:1090406/140‑1 (MQ=255)
                                                                                                       gCCGTGTTGAAAGGTGACATGGGTGACGTGCATCATTTTGCCGATGACGTTATCGCCCAGCGCGGCGTGCGGCACGGGCATTTGCAGTGCTTGCCGAAGGAAGATTGAGTCTATTATTGGGTCCTTTGGTGaa                                      <  1:1229125/133‑1 (MQ=255)
                                                                                                       gCCGTGTTGAAAGGTGACATGGGTGACGTGCATCATTTTGCCGATGACGTTATCGCCCAGCGCGGCGTGCGGCACGGGCATTTGCAGTGCTTGCCGAAGGAAGATTGAGTCTATTATTGGGTCCTTTGGTGaa                                      >  2:1229125/1‑133 (MQ=255)
                                                                                                       gCCGTGTTGAAAGGTGACATGGGTGACGTGCATCATTTTGCCGATGACGTTATCGCCCAGCGCGGCGTGCGGCACGGGCATTTGCAGTGCTTGCCGAAGGAAGATTGAGTCTATTATTGGGTCCTTTGGTGAACGATcaa                               >  1:1031265/1‑140 (MQ=255)
                                                                                                        ccGTGTTGAAAGGTGACATGGGTGACGTGCATCATTTTGCCGATGACGTTATCGCCCAGCGCGGCGTGCGGCACGGGCATTTGCAGTGCTTGCCGAAGGAAGATTGAGTCTATTATTGGGTCCTTTGGTGAACGATcaac                              >  1:68063/1‑140 (MQ=255)
                                                                                                          gtgtTGAAAGGTGACATGGGTGACGTGCATCATTTTGCCGATGACGTTATCGCCCAGCGCGGCGTGCGGCACGGGCATTTGCAGTGCTTGCCGAAGGAAGATTGAGTCTATTATTGGGTCCTTTGGTGAACGATcaacaa                            <  1:362459/140‑1 (MQ=255)
                                                                                                           tgtTGAAAGGTGACATGGGTGACGTGCATCATTTTGCCGATGACGTTATCGCCCAGCGCGGCGTGCGGCACGGGCATTTGCAGTGCTTGCCGAAGGAAGATTGAGTCTATTATTGGGTCCTTTGGTGAACGATCAACaaa                           <  1:248478/140‑1 (MQ=255)
                                                                                                             ttGAAAGGTGACATGGGTGACGTGCATCATTTTGCCGATGACGTTATCGCCCAGCGCGGCGTGCGGCACGGGCATTTGCAATGCTTGCCGAAGGAAGATTGAGTCTATTATTGGGTCCTTTGGTGAACGATCAACAAAgg                         <  1:71747/140‑1 (MQ=255)
                                                                                                               gAAAGGTGACATGGGTGACGTGCATCATTTTGCCGATGACGTTATCGCCCAGCGCGGCGTGCGGCACGGGCATTTGCAGTGCTTGCCGAAGGAAGATTGAGTCTATTATTGGGTCCTTTGGTGAACGATCAACAAAGGGc                       <  2:1031265/140‑1 (MQ=255)
                                                                                                                aaaGGTGACATGGGTGACGTGCATCATTTTGCCGATGACGTTATCGCCCAGCGCGGCGTGCGGCACGGGCATTTGCAGTGCTTGCCGAAGGAAGATTGAGTCTATTATTGGGTCCTTTGGTGAACGATCAACAAAGGGcc                      <  1:1089359/140‑1 (MQ=255)
                                                                                                                aaaGGTGACATGGGTGACGTGCATCATTTTGCCGATGACGTTATCGCCCAGCGCGGCGTGCGGCACGGGCATTTGCAGTGCTTGCCGAAGGAAGATTGAGTCTATTATTGGGTCCTTTGGTGAACGATCAACAAAGGGcc                      <  1:683239/140‑1 (MQ=255)
                                                                                                                aaaGGTGACATGGGTGACGTGCATCATTTTGCCGATGACGTTATCGCCCAGCGCGGCGTGCGGCACGGGCATTTGCAGTGCTTGCCGAAGGAAGATTGAGTCTATTATTGGGTCCTTTGGTGAACGATCAACAAAGGGcc                      <  1:1158952/140‑1 (MQ=255)
                                                                                                                  aGGTGACATGGGTGACGTGCATCATTTTGCCGATGACGTTATCGCCCAGCGCGGCGTGCGGCACGGGCATTTGCAGTGCTTGCCGAAGGAAGATTGAGTCTATTATTGGGTCCTTTGGTGAACg                                    >  2:714561/1‑124 (MQ=255)
                                                                                                                       aCATGGGTGACGTGCATCATTTTGCCGATGACGTTATCGCCCAGCGCGGCGTGCGGCACGGGCATTTGCAGTGCTTGCCGAAGGAAGATTGAGTCTATTATTGGGTCCTTTGGTGAACGATCAACAAAGGGCCACTAGca               >  1:456653/1‑140 (MQ=255)
                                                                                                                       aCATGGGTGACGTGCATCATTTTGCCGATGACGTTATCGCCCAGCGCGGCGTGCGGCACGGGCATTTGCAGTGCTTGCCGAAGGAAGATTGAGTCTATTATTGGGTCCTTTGGTGAACGATCAACAAAGGGCCACTAGca               <  2:676192/140‑1 (MQ=255)
                                                                                                                       aCATGGGTGACGTGCATCATTTTGCCGATGACGTTATCGCCCAGCGCGGCGTGCGGCACGGGCATTTGCAGTGCTTGCCGAAGGAAGATTGAGTCTATTATTGGGTCCTTTGGTGAACGATCAACAAAGGGCCACTAGca               <  1:1143721/140‑1 (MQ=255)
                                                                                                                       aCATGGGTGACGTGCATCATTTTACCGATGACGTTATCGCCCAGCGCGGCGTGCGGCACGGGCATTTGCAGTGCTTGCCGAAGGAAGATTGAGTCTATTATTGGGTCCTTTGGTGAACGATCAACAAAGGGCCACTAGca               >  1:468000/1‑140 (MQ=255)
                                                                                                                           gggTGACGTGCATCATTTTGCCGATGACGTTATCGCCCAGCGCGGCGTGCGGCACGGGCATTTGCAGTGCTTGCCGAAGGAAGATTGAGTCTATTATTGGGTCCTTTGGTGAACGATCAACAAAGGGCCACTAGCACAcc           >  1:981091/1‑140 (MQ=255)
                                                                                                                              tGACGTGCATCATTTTGCCGATGACGTTATCGCCCAGCGCGGCGTGCGGCACGGGCATTTGCAGTGCTTGCCGAAGGAAGATTGAGTCTATTATTGGGTCCTTTGGTGAACGATCAACAAAGGGCCACTAGCACACCTGa        <  2:658280/140‑1 (MQ=255)
                                                                                                                              tGACGTGCATCATTTTGCCGATGACGTTATCGCCCAGCGCGGCGTGCGGCACGGGCATTTGCAGTGCTTGCCGAAGGAAGATTGAGTCTATTATTGGGTCCTTTGGTGAACGATCAACAAAGGGCCACTAGCACACCTGa        >  1:502712/1‑140 (MQ=255)
                                                                                                                                aCGTGCATCATTTTGCCGATGACGTTATCGCCCAGCGCGGCGTGCGGCACGGGCATTTGCAGTGCTTGCCGAAGGAAGATTGAGTCTATTATTGGGTCCTTTGGTGAACGATCAACAAAGGGCCACTAGCACACCTGAtt      <  2:1358708/140‑1 (MQ=255)
                                                                                                                                 cGTGCATCATTTTGCCGATGACGTTATCGCCCAGCGCGGCGTGCGGCACGGGCATTTGCAGTGCTTGCCGAAGGAAGATTGAGTCTATTATTGGGTCCTTTGGTGAACGATCAACAAAGGGCCACTAGCACACCTGATTc     >  1:575847/1‑140 (MQ=255)
                                                                                                                                   tGCATCATTTTGCCGATGACGTTATCGCCCAGCGCGGCGTGCGGCACGGGCATTTGCAGTGCTTGCCGAAGGAAGATTGAGTCTATTATTGGGTCCTTTGGTGAACGATCAACAAAGGGCCACTAGCACACCTGATTCct   <  2:398834/140‑1 (MQ=255)
                                                                                                                                   tGCATCATTTTGCCGATGACGTTATCGCCCAGCGCGGCGTGCGGCACGGGCATTTGCAGTGCTTGCCGAAGGAAGATTGAGTCTATTATTGGGTCCTTTGGTGAACGATCAACAAAGGGCCACTAGCACACCTGATTCct   <  2:449815/140‑1 (MQ=255)
                                                                                                                                    gcatcaTTTTGCCGATGACGTTATCGCCCAGCGCGGCGTGCGGCACGGGCATTTGCAGTGCTTGCCGAAGGAAGATTGAGTCTATTATTGGGTCCTTTGGTGAACGATCAACAAAGGGCCACTAGCACACCTGATTCctc  <  2:210808/140‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                        
ACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCCGTCGCCACGCTGCATGTGCACATCAACCACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGTGACGTGCAGCATTTTGCCGATGACGTTATCGCCCAGCGCGGCGTGCGGCACGGGCATTTGCAGTGCTTGCCGAAGGAAGATTGAGTCTATTATTGGGTCCTTTGGTGAACGATCAACAAAGGGCCACTAGCACACCTGATTCCTC  >  NC_000913/3618779‑3619050

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑