Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,847,258	G→T	intergenic (‑91/+47)	uhpT ← / ← uhpC	hexose‑6‑phosphate:phosphate antiporter/inner membrane protein sensing glucose‑6‑phosphate

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,847,258	0	G	T	100.0%	270.4 / NA	77	intergenic (‑91/+47)	uhpT/uhpC	hexose‑6‑phosphate:phosphate antiporter/inner membrane protein sensing glucose‑6‑phosphate
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (37/40);  total (37/40)

GAAGGTCCAGGGTCGGCTTGCGAACCTGGTTTAAGAAAGCCAGCATGGGTTACTCCTGAAATGAATACCTGCGTGAGGCATGCATTGTAAAAATCAGCAGCAAATCAGGCCTGAGATAGCGTCCAGGCAAAACCTGGAAAATTTCTTAGTTTTGCCGGATATGAAGTGAAAAGGTGAGATGCATCACGCTTCGCGCGGTGTCTGGGCGTTCAAAAAGGGCAGTAACAGCAGTGCGGAAATCCCGGCGGCGATAGAGATAACCACAAAAAATC  >  NC_000913/3847122‑3847393                                                                                                                                         |                                                                                                                                        gAAGGTCCAGGGTCGGCTTGCGAACCTGGTTTAAGAAAGCCAGCATGGGTTACTCCTGAAATGAATACCTGCGTGAGGCATGCATTGTAAAAATCAGCAGCAAATCAGGCCTGAGATAGCGTCCAGGCAAAACCTGTaaa                                                                                                                                      >  2:1416638/1‑140 (MQ=255)                gCTTGCGAACCTGGTTTAAGAAAGCCAGCATGGGTTACTCCTGAAATGAATACCTGCGTGAGGCATGCATTGTAAAAATCAGCAGCAAATCAGGCCTGAGATAGCGTCCAGGCAAAACCTGTAAAATTTCTTAGTTTTGc                                                                                                                       >  1:910034/1‑140 (MQ=255)                gCTTGCGAACCTGGTTTAAGAAAGCCAGCATGGGTTACTCCTGAAATGAATACCTGCGTGAGGCATGCATTGTAAAAATCAGCAGCAAATCAGGCCTGAGATAGCGTCCAGGCAAAACCTGTAAAATTTCTTAGTTTTGc                                                                                                                       <  2:300157/140‑1 (MQ=255)                gCTTGCGAACCTGGTTTAAGAAAGCCAGCATGGGTTACTCCTGAAATGAATACCTGCGTGAGGCATGCATTGTAAAAATCAGCAGCAAATCAGGCCTGAGATAGCGTCCAGGCAAAACCTGTAAAATTTCTTAGTTTTGc                                                                                                                       >  1:450366/1‑140 (MQ=255)                gCTTGCGAACCTGGTTTAAGAAAGCCAGCATGGGTTACTCCTGAAATGAATACCTGCGTGAGGCATGCATTGTAAAAATCAGCAGCAAATCAGGCCTGAGATAGCGTCCAGGCAAAACCTGTAAAATTTCTTAGTTTTGc                                                                                                                       >  1:1332883/1‑140 (MQ=255)                gCTTGCGAACCTGGTTTAAGAAAGCCAGCATGGGTTACTCCTGAAATGAATACCTGCGTGAGGCATGCATTGTAAAAATCAGCAGCAAATCAGGCCTGAGATAGCGTCCAGGCAAAACCTGTAAAATTTCTTAGTTTTGc                                                                                                                       >  1:1002869/1‑140 (MQ=255)                gCTTGCGAACCTGGTTTAAGAAAGCCAGCATGGGTTACTCCTGAAATGAATACCTGCGTGAGGCATGCATTGTAAAAATCAGCAGCAAATCAGGCCTGAGATAGCGTCCAGGCAAAACCTGTAAAATTTCTTAGTTTTGc                                                                                                                       >  2:308990/1‑140 (MQ=255)                gCTTGCGAACCTGGTTTAAGAAAGCCAGCATGGGTTACTCCTGAAATGAATACCTGCGTGAGGCATGCATTGTAAAAATCAGCAGCAAATCAGGCCTGAGATAGCGTCCAGGCAAAACCTGTAAAATTTCTTAGTTTTGc                                                                                                                       <  1:1416638/140‑1 (MQ=255)                gCTTGCGAACCTGGTTTAAGAAAGCCAGCATGGGTTACTCCTGAAATGAATACCTGCGTGAGGCATGCATTGTAAAAATCAGCAGCAAATCAGGCCTGAGATAGCGTCCAGGCAAAACCTGTAAAATTTCTTAGTTTTGc                                                                                                                       <  1:14330/140‑1 (MQ=255)                gCTTGCGAACCTGGTTTAAGAAAGCCAGCATGGGTTACTCCTGAAATGAATACCTGCGTGAGGCATGCATTGTAAAAATCAGCAGCAAATCAGGCCTGAGATAGCGTCCAGGCAAAACCTGTAAAATTTCTTAGTTTTGc                                                                                                                       <  1:1448505/140‑1 (MQ=255)                    gCGAACCTGGTTTAAGAAAGCCAGCATGGGTTACTCCTGAAATGAATACCTGCGTGAGGCATGCATTGTAAAAATCAGCAGCAAATCAGGCCTGAGATAGCGTCCAGGCAAAACCTGTAAAATTTCTTAGTTTTGCCGGa                                                                                                                   >  2:313746/1‑140 (MQ=255)                     cGAACCTGGTTTAAGAAAGCCAGCATGGGTTACTCCTGAAATGAATACCTGCGTGAGGCATGCATTGTAAAAATCAGCAGCAAATCAGGCCTGAGATAGCGTCCAGGCAAAACCTGTAAAATTTCTTAGTTTTGCCGGat                                                                                                                  <  2:1018327/140‑1 (MQ=255)                     cGAACCAGGTTTAAGAAAGCCAGCATGGGTTACTCCTGAAATGAATACCTGCGTGAGGCATGCATTGTAAAAATCAGCAGCAAATCAGGCCTGAGATAGCGTCCAGGCAAAACCTGTAAAATTTCTTAGTCTTGCCGGat                                                                                                                  <  2:180304/140‑1 (MQ=255)                               ttAAGAAAGCCAGCATGGGTTACTCCTGAAATGAATACCTGCGTGAGGCATGCATTGTAAAAATCAGCAGCAAATCAGGCCTGAGATAGCGTCCAGGCAAAACCTGTAAAATTTCTTAGTTTTGCCGGATATGAAGTGaa                                                                                                        <  1:308990/140‑1 (MQ=255)                                  aGAAAGCCAGCATGGGTTACTCCTGAAATGAATACCTGCGTGAGGCATGCATTGTAAAAATCAGCAGCAAATCAGGCCTGAGATAGCGTCCAGGCAAAACCTGTAAAATTTCTTAGTTTTGCCGGATATGAAGTGAAAAg                                                                                                     <  1:741538/140‑1 (MQ=255)                                    aaaGCCAGCATGGGTTACTCCTGAAATGAATA‑CTGCGTGAGGCATGCATTGTAAAAATCAGCAGCAAATCAGGCCTGAGATAGCGTCCAGGCAAAACCTGTAAAATTTCTTAGTTTTGCCGGATATGAAGTGAAAAGGTg                                                                                                  <  1:1238847/140‑1 (MQ=255)                                       gCCAGCATGGGTTACTCCTGAAATGAATACCTGCGTGAGGCATGCATTGTAAAAATCAGCAGCAAATCAGGCCTGAGATAGCGTCCAGGCAAAACCTGTAAAATTTCTTAGTTTTGCCGGATATGAAGTGAAAAGGTgag                                                                                                <  1:482986/140‑1 (MQ=255)                                       gCCAGCATGGGTTACTCCTGAAATGAATACCTGCGTGAGGCATGCATTGTAAAAATCAGCAGCAAATCAGGCCTGAGATAGCGTCCAGGCAAAACCTGTAAAATTTCTTAGTTTTGCCGGATATGAAGTGAAAAGGTgag                                                                                                >  2:170750/1‑140 (MQ=255)                                       gCCAGCATGGGTTACTCCTGAAATGAATACCTGCGTGAGGCATGCATTGTAAAAATCAGCAGCAAATCAGGCCTGAGATAGCGTCCAGGCAAAACCTGTAAAATTTCTTAGTTTTGCCGGATATGAAGTGAAAAGGTgag                                                                                                >  1:563349/1‑140 (MQ=255)                                       gCCAGCATGGGTTACTCCTGAAATGAATACCTGCGTGAGGCATGCATTGTAAAAATCAGCAGCAAATCAGGCCTGAGATAGCGTCCAGGCAAAACCTGTAAAATTTCTTAGTTTTGCCGGATATGAAGTGAAAAGGTgag                                                                                                >  1:608207/1‑140 (MQ=255)                                               gggTTACTCCTGAAATGAATACCTGCGTGAGGCATGCATTGTAAAAATCAGCAGCAAATCAGGCCTGAGATAGCGTCCAGGCAAAACCTGTAAAATTTCTTAGTTTTGCCGGATATGAAGTGAAAAGGTGAGATGCATCa                                                                                        >  1:1444063/1‑140 (MQ=255)                                                    aCTCCTGAAATGAATACCTGCGTGAGGCATGCATTGTAAAAATCAGCAGCAAATCAGGCCTGAGATAGCGTCCAGGCAAAACCTGTAAAATTTCTTAGTTTTGCCGGATATGAAGTGAAAAGGTGAGATGCATCACGCtt                                                                                   <  1:880334/140‑1 (MQ=255)                                                    aCTCCTGAAATGAATACCTGCGTGAGGCATGCATTGTAAAAATCAGCAGCAAATCAGGCCTGAGATAGCGTCCAGGCAAAACCTGTAAAATTTCTTAGTTTTGCCGGATATGAAGTGAAAAGGTGAGATGCATCACGCtt                                                                                   >  1:596899/1‑140 (MQ=255)                                                      tCCTGAAATGAATACCTGCGTGAGGCATGCATTGTAAAAATCAGCAGCAAATCAGGCCTGAGATAGCGTCCAGGCAAAACCTGTAAAATTTCTTAGTTTTGCCGGATATGAAGTGAAAAGGTGAGATGCATCACGCTTcg                                                                                 <  1:220946/140‑1 (MQ=255)                                                        cTGAAATGAATACCTGCGTGAGGCATGCATTGTAAAAATCAGCAGCAAATCAGGCCTGAGATAGCGTCCAGGCAAAACCTGTAAAATTTCTTAGTTTTGCCGGATATGAAGTGAAAAGGTGAGATGCATCGCGCTTcgcg                                                                               <  1:313746/140‑1 (MQ=255)                                                        cTGAAATGAATACCTGCGTGAGGCATGCATTGTAAAAATCAGCAGCAAATCAGGCCTGAGATAGCGTCCAGGCAAAACCTGTAAAATTTCTTAGTTTTGCCGGATATGAAGTGAAAAGGTGAGATGCATCACGCTTcgcg                                                                               <  1:170750/140‑1 (MQ=255)                                                        cTGAAATGAATACCTGCGTGAGGCATGCATTGTAAAAATCAGCAGCAAATCAGGCCTGAGATAGCGTCCAGGCAAAACCTGTAAAATTTCTTAGTTTTGCCGGATATGAAGTGAAAAGGTGAGATGCATCACGCTTcgcg                                                                               <  1:322120/140‑1 (MQ=255)                                                        cTGAAATGAATACCTGCGTGAGGCATGCATTGTAAAAATCAGCAGCAAATCAGGCCTGAGATAGCGTCCAGGCAAAACCTGTAAAATTTCTTAGTTTTGCCGGATATGAAGTGAAAAGGTGAGATGCATCACGCTTcgcg                                                                               <  1:1143774/140‑1 (MQ=255)                                                          gAAATGAATACCTGCGTGAGGCATGCATTGTAAAAATCAGCAGCAAATCAGGCCTGAGATAGCGTCCAGGCAAAACCTGTAAAATTTCTTAGTTTTGCCGGATATGAAGTGAAAAGGTGAGATGCATCACGCTTcgcgcg                                                                             >  1:756919/1‑140 (MQ=255)                                                          gAAATGAATACCTGCGTGAGGCATGCATTGTAAAAATCAGCAGCAAATCAGGCCTGAGATAGCGTCCAGGCAAAACCTGTAAAATTTCTTAGTTTTGCCGGATATGAAGTGAAAAGGTGAGATGCATCACGCTTcgcgcg                                                                             <  1:617394/140‑1 (MQ=255)                                                          gAAATGAATACCTGCGTGAGGCATGCATTGTAAAAATCAGCAGCAAATCAGGCCTGAGATAGCGTCCAGGCAAAACCTGTAAAATTTCTTAGTTTTGCCGGATATGAAGTGAAAAGGTGAGATGCATCACGCTTcgcgcg                                                                             <  1:1410517/140‑1 (MQ=255)                                                               gAATACCTGCGTGAGGCATGCATTGTAAAAATCAGCAGCAAATCAGGCCTGAGATAGCGTCCAGGCAAACCCTGTAAAATTTCTTAGTTTTGCCGGATATGAAGTGAAAAGGTGAGATGCATCACGCTTcgcg                                                                               <  2:970701/133‑1 (MQ=255)                                                               gAATACCTGCGTGAGGCATGCATTGTAAAAATCAGCAGCAAATCAGGCCTGAGATAGCGTCCAGGCAAAACCTGTAAAATTTCTTAGTTTTGCCGGATATGAAGTGAAAAGGTGAGATGCATCACGCTTcgcg                                                                               >  1:970701/1‑133 (MQ=255)                                                               gAATACCTGCGTGAGGCATGCATTGTAAAAATCAGCAGCAAATCAGGCCTGAGATAGCGTCCAGGCAAAACCTGTAAAATTTCTTAGTTTTGCCGGATATGAAGTGAAAAGGTGAGATGCATCACGCTTCGCGCGGTGTc                                                                        <  1:802925/140‑1 (MQ=255)                                                                aaTACCTGCGTGAGGCATACATTGTAAAAATCAGCAGCAAATCAGGCCTGAGATAGCGTCCAGGCAAAACCTGTAAAATTTCTTAGTTTTGCCGGATATGAAGTGAAAAGGTGAGATGCATCACGCTTcgcg                                                                               <  2:91649/132‑1 (MQ=255)                                                                aaTACCTGCGTGAGGCATACATTGTAAAAATCAGCAGCAAATCAGGCCTGAGATAGCGTCCAGGCAAAACCTGTAAAATTTCTTAGTTTTGCCGGATATGAAGTGAAAAGGTGAGATGCATCACGCTTcgcg                                                                               >  1:91649/1‑132 (MQ=255)                                                                  tACCTGCGTGAGGCATGCATTGTAAAAATCAGCAGCAAATCAGGCCTGAGATAGCGTCCAGGCAAAACCTGTAAAATTTCTTAGTTTTGCCGGATATGAAGTGAAAAGGTGAGATGCATCACGCTTCGCGCGGTGTCTgg                                                                     >  1:1381041/1‑140 (MQ=255)                                                                  tACCTGCGTGAGGCATGCATTGTAAAAATCAGCAGCAAATCAGGCCTGAGATAGCGTCCAGACAAAACCTGTAAAATTTCTTAGTTTTGCCGGATATGAAGTGAAAAGGTGAGATGCATCACGCt                                                                                    <  2:1333631/125‑1 (MQ=255)                                                                  tACCTGCGTGAGGCATGCATTGTAAAAATCAGCAGCAAATCAGGCCTGAGATAGCGTCCAGACAAAACCTGTAAAATTTCTTAGTTTTGCCGGATATGAAGTGAAAAGGTGAGATGCATCACGCt                                                                                    >  1:1333631/1‑125 (MQ=255)                                                                     cTGCGTGAGGCATGCATTGTAAAAATCAGCAGCAAATCAGGCCTGAGATAGCGTCCAGGCAAAACCTGTAAAATTTCTTAGTTTTGCCGGATATGAAGTGAAAAGGTGAGATGCATCACGCTTCGCGCGGTGTCTGGGCg                                                                  >  1:739632/1‑140 (MQ=255)                                                                      tGCGTGAGGCATGCATTGTAAAAATCAACAGCAAATCAGGCCTGAGATAGCGTCCAGGCAAAACCTGTAAAATTTCTTAGTTTTGCCGGATATGAAGTGAAAAGGTGAGATGCATCACGCTTCGCGCGGTGTCTGGGCGt                                                                 <  1:1012252/140‑1 (MQ=255)                                                                       gCGTGAGGCATGCATTGTAAAAATCAGCAGCAAATCAGGCCTGAGATAGCGTCCAGGCAAAACCTGTAAAATTTCTTAGTTTTGCCGGATATGAAGTGAAAAGGTGAGATGCATCACGCTTCGCGCGGTGTCTGGGCGtt                                                                >  2:1375795/1‑140 (MQ=255)                                                                          tGAGGCATGCATTGTAAAAATCAGCAGCAAATCAGGCCTGAGATAGCGTCCAGGCAAAACCTGTAAAATTTCTTAGTTTTGCCGGATATGAAGTGAAAAGGTGAGATGCATCACGCTTcgcg                                                                               <  2:845/122‑1 (MQ=255)                                                                          tGAGGCATGCATTGTAAAAATCAGCAGCAAATCAGGCCTGAGATAGCGTCCAGGCAAAACCTGTAAAATTTCTTAGTTTTGCCGGATATGAAGTGAAAAGGTGAGATGCATCACGCTTcgcg                                                                               >  1:845/1‑122 (MQ=255)                                                                          tGAGGCATGCATTGTAAAAATCAGCAGCAAATCAGGCCTGAGATAGCGTCCAGGCAAAACCTGTAAAATTTCTTAGTTTTGCCGGATATGAAGTGAAAAGGTGAGATGCATCACGCTTCGCGCGGTGTCTGGGCGTTCaa                                                             >  2:986249/1‑140 (MQ=255)                                                                               catgcatTGTAAAAATCAGCAGCAAATCAGGCCTGAGATAGCGTCCAGGCAAAACCTGTAAAATTTCTTAGTTTTGCCGGATATGAAGTGAAAAGGTGAGATGCATCACGCTTCGCGCGGTGTCTGGGCGTTCAAAAAgg                                                        >  1:1212328/1‑140 (MQ=255)                                                                                 tgcatTGTAAAAATCAGCAGCAAATCAGGCCTGAGATAGCGTCCAGGCAAAACCTGTAAAATTTCTTAGTTTTGCCGGATATGAAGTGAAAAGGTGAGATGCATCACGCTTCGCGCGGTGTCTGGGCGTTCAAAAAGGGc                                                      <  2:1381041/140‑1 (MQ=255)                                                                                 tgcatTGTAAAAATCAGCAGCAAATCAGGCCTGAGATAGCGTCCAGGCAAAACCTGTAAAATTTCTTAGTTTTGCCGGATATGAAGTGAAAAGGTGAGATGCATCACGCTTCGCGCGGTGTCTGGGCGTTCAAAAAGGGc                                                      <  2:756919/140‑1 (MQ=255)                                                                                 tgcatTGTAAAAATCAGCAGCAAATCAGGCCTGAGATAGCGTCCAGGCAAAACCTGTAAAATTTCTTAGTTTTGCCGGATATGAAGTGAAAAGGTGAGATGCATCACGCTTCGCGCGGTGTCTGGGCGTTCAAAAAGGGc                                                      <  1:466280/140‑1 (MQ=255)                                                                                  gcatTGTAAAAATCAGCAGCAAATCAGGCCTGAGATAGCGTCCAGGCAAAACCTGTAAAATTTCTTAGTTTTGCCGGATATGAAGTGAAAAGGTGAGATGCATCACGCTTCGCGCGGTGTCTGGGCGTTCAAAAAGGGCa                                                     >  2:241942/1‑140 (MQ=255)                                                                                   catTGTAAAAATCAGCAGCAAATCAGGCCTGAGATAGCGTCCAGGCAAAACCTGTAAAATTTCTTAGTTTTGCCGGATATGAAGTGAAAAGGTGAGATGCATCACGCTTCGCGCGGTGTCTGGGCGTTCAAAAAggg                                                       <  1:725611/137‑1 (MQ=255)                                                                                   catTGTAAAAATCAGCAGCAAATCAGGCCTGAGATAGCGTCCAGGCAAAACCTGTAAAATTTCTTAGTTTTGCCGGATATGAAGTGAAAAGGTGAGATGCATCACGCTTCGCGCGGTGTCTGGGCGTTCAAAAAggg                                                       >  2:725611/1‑137 (MQ=255)                                                                                                agcagcAAATCAGGCCTGAGATAGCGTCCAGGCAAAACCTGTAAAATTTCTTAGTTTTGCCGGATATGAAGTGAAAAGGTGAGATGCATCACGCTTCGCGCGGTGTCTGGGCGTTCAAAAAGGGCAGTAACAGCAGTGCg                                       >  1:735761/1‑140 (MQ=255)                                                                                                agcagcAAATCAGGCCTGAGATAGCGTCCAGGCAAAACCTGTAAAATTTCTTAGTTTTGCCGGATATGAAGTGAAAAGGTGAGATGCATCACGCTTCGCGCGGTGTCTGGGCGTTCAAAAAGGGCAGTAACAGCAGTGCg                                       >  2:79056/1‑140 (MQ=255)                                                                                                agcagcAAATCAGGCCTGAGATAGCGTCCAGGCAAAACCTGTAAAATTTCTTAGTTTTGCCGGATATGAAGTGAAAAGGTGAGATGCATCACGCTTCGCGCGGTGTCTGGGCGTTCAAAAAGGGCAGTAACAGCAGTGCg                                       >  1:65578/1‑140 (MQ=255)                                                                                                agcagcAAATCAGGCCTGAGATAGCGTCCAGGCAAAACCTGTAAAATTTCTTAGTTTTGCCGGATATGAAGTGAAAAGGTGAGATGCATCACGCTTCGCGCGGTGTCTGGGCGTTCAAAAAGGGCAGTAACAGCAGTGCg                                       >  2:518111/1‑140 (MQ=255)                                                                                                agcagcAAATCAGGCCTGAGATAGCGTCCAGGCAAAACCTGTAAAATTTCTTAGTTTTGCCGGATATGAAGTGAAAAGGTGAGATGCATCACGCTTCGCGCGGTGTCTGGGCGTTCAAAAAGGGCAGTAACAGCAGTGCg                                       >  2:292207/1‑140 (MQ=255)                                                                                                      aaaTCAGGCCTGAGATAGCGTCCAGGCAAAACCTGTAAAATTTCTTAGTTTTGCCGGATATGAAGTGAAAAGGTGAGATGCATCACGCTTCGCGCGGTGTCTGGGCGTTCAAAAAGGGCAGTAACAGCAGTGCGGAAATc                                 <  2:450366/140‑1 (MQ=255)                                                                                                           aGGCCTGAGATAGCGTCCAGGCAAAACCTGTAAAATTTCTTAGTTTTGCCGGATATGAAGTGAAAAGGTGAGATGCATCACGCTTCGCGCGGTGTCTGGGCGTTCAAAAAGGGCAGTAACAGCAGTGCGGAAATCCcggc                            <  2:420248/140‑1 (MQ=255)                                                                                                           aGGCCTGAGATAGCGTCCAGGCAAAACCTGTAAAATTTCTTAGTTTTGCCGGATATGAAGTGAAAAGGTGAGATGCATCACGCTTCGCGCGGTGTCTGGGCGTTCAAAAAGGGCAGTAACAGCAGTGCGGAAATCCcggc                            <  2:466198/140‑1 (MQ=255)                                                                                                           aGGCCTGAGATAGCGTCCAGGCAAAACCTGTAAAATTTCTTAGTTTTGCCGGATATGAAGTGAAAAGGTGAGATGCATCACGCTTCGCGCGGTGTCTGGGCGTTCAAAAAGGGCAGTAACAGCAGTGCGGAAATCCcggc                            <  1:518111/140‑1 (MQ=255)                                                                                                               cTGAGATAGCGTCCAGGCAAAACCTGTAAAATTTCTTAGTTTTGCCGGATATGAAGTGAAAAGGTGAGATGCATCACGCTTCGCGCGGTGTCTGGGCGTTCAAAAAGGGCAGTAACAGCAGTGCGGAAATCCCGgcggcg                        <  2:735761/140‑1 (MQ=255)                                                                                                                  agaTAGCGTCCAGGCAAAACCTGTAAAATTTCTTAGTTTTGCCGGATATGAAGTGAAAAGGTGAGATGCATCACGCTTCGCGCGGTGTCTGGGCGTTCAAAAAGGGCAGTACCAGCAGTGCGGAAATCCCGGCGGCGATa                     <  2:1332883/140‑1 (MQ=255)                                                                                                                  agaTAGCGTCCAGGCAAAACCTGTAAAATTTCTTAGTTTTGCCGGATATGAAGTGAAAAGGTGAGATGCATCACGCTTCGCGCGGTGTCTGGGCGTTCAAAAAGGGCAGTAACAGCAGTGCGGAAATCCCGGCGGCGATa                     >  1:333672/1‑140 (MQ=255)                                                                                                                      aGCGTCCAGGCAAAACCTGTAAAATTTCTTAGTTTTGCCGGATATGAAGTGAAAAGGTGAGATGCATCACGCTTCGCGCGGTGTCTGGGCGTTCAAAAAGGGCAGTAACAGCAGTGCGGAAATCCCGGCGGCGATAgaga                 <  1:241942/140‑1 (MQ=255)                                                                                                                       gCGTCCAGGCAAAACCTGTAAAATTTCTTAGTTTTGCCGGATATGAAGTGAAAAGGTGAGATGCATCACGCTTCGCGCGGTGTCTGGGCGTTCAAAAAGGGCAGTAACAGCAGTGCGGAAATccc                               >  1:257951/1‑125 (MQ=255)                                                                                                                       gCGTCCAGGCAAAACCTGTAAAATTTCTTAGTTTTGCCGGATATGAAGTGAAAAGGTGAGATGCATCACGCTTCGCGCGGTGTCTGGGCGTTCAAAAAGGGCAGTAACAGCAGTGCGGAAATccc                               <  2:257951/125‑1 (MQ=255)                                                                                                                       gCGTCCAGGCAAAACCTGTAAAATTTCTTAGTTTTGCCGGATATGAAGTGAAAAGGTGAGATGCATCACGCTTCGCGCGGTGTCTGGGCGTTCAAAAAGGGCAGTAACAGCAGTGCGGAAATCCcggcg                           <  2:1038859/129‑1 (MQ=255)                                                                                                                       gCGTCCAGGCAAAACCTGTAAAATTTCTTAGTTTTGCCGGATATGAAGTGAAAAGGTGAGATGCATCACGCTTCGCGCGGTGTCTGGGCGTTCAAAAAGGGCAGTAACAGCAGTGCGGAAATCCcggcg                           >  1:1038859/1‑129 (MQ=255)                                                                                                                       gCGTCCAGGCAAAACCTGTAAAATTTCTTAGTTTTGCCGGATATGAAGTGAAAAGGTGAGATGCATCACGCTTCGCGCGGTGTCTGGGCGTTCAAAAAGGGCAGTAACAGCAGTGCGGAAATCCCGGCGGCGATAGAGAt                >  2:711672/1‑140 (MQ=255)                                                                                                                       gCGTCCAGGCAAAACCTGTAAAATTTCTTAGTTTTGCCGGATATGAAGTGAAAAGGTGAGATGCATCACGCTTCGCGCGGTGTCTGGGCGTTCAAAAAGGGCAGTAACAGCAGTGCGGAAATCCCGGCGGCGATAGAGAt                >  2:476782/1‑140 (MQ=255)                                                                                                                        cGTCCAGGCAAAACCTGTAAAATTTCTTAGTTTTGCCGGATATGAAGTGAAAAGGTGAGATGCATCACGCTTCGCGCGGTGTCTGGGCGTTCAAAAAGGGCAGTAACAGCAGTGCGGAAATCCCGGCGGCGATAGAGATa               >  1:509261/1‑140 (MQ=255)                                                                                                                              ggCAAAACCTGTAAAATTTCTTAGTTTTGCCGGATATGAAGTGAAAAGGTGAGATGCATCACGCTTCGCGCGGTGTCTGGGCGTTCAAAAAGGGCAGTAACAGCAGTGCGGAAATCCCGGCGGCGATAGAGATAACcac          >  2:829797/1‑139 (MQ=255)                                                                                                                              ggCAAAACCTGTAAAATTTCTTAGTTTTGCCGGATATGAAGTGAAAAGGTGAGATGCATCACGCTTCGCGCGGTGTCTGGGCGTTCAAAAAGGGCAGTAACAGCAGTGCGGAAATCCCGGCGGCGATAGAGATAACcac          <  1:829797/139‑1 (MQ=255)                                                                                                                                    aCCTGTAAAATTTCTTAGTTTTGCCGGATATGAAGTGAAAAGGTGAGATGCATCACGCTTCGCGCGGTGTCTGGGCGTTCAAAAAGGGCAGTAACAGCAGTGCGGAAATCCCGGCGGCGATAGAGATAACCACAAAAAAt   >  2:1363365/1‑140 (MQ=255)                                                                                                                                     ccTGTAAAATTTCTTAGTTTTGCCGGATATGAAGTGAAAAGGTGAGATGCATCACGCTTCGCGCGGTGTCTGGGCGTTCAAAAAGGGCAGTAACAGCAGTGCGGAAATCCCGGCGGCGATAGAGATAACCACAAAAAATc  <  2:596899/140‑1 (MQ=255)                                                                                                                                     ccTGTAAAATTTCTTAGTTTTGCCGGATATGAAGTGAAAAGGTGAGATGCATCACGCTTCGCGCGGTGTCTGGGCGTTCAAAAAGGGCAGTAACAGCAGTGCGGAAATCCCGGCGGCGATAGAGATAACCACAAAAAATc  <  2:910034/140‑1 (MQ=255)                                                                                                                                     ccTGTAAAATTTCTTAGTTTTGCCGGATATGAAGTGAAAAGGTGAGATGCATCACGCTTCGCGCGGTGTCTGGGCGTTCAAAAAGGGCAGTAACAGCAGTGCGGAAATCCCGGCGGCGATAGAGATAACCACAAAAAATc  <  2:333672/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                        GAAGGTCCAGGGTCGGCTTGCGAACCTGGTTTAAGAAAGCCAGCATGGGTTACTCCTGAAATGAATACCTGCGTGAGGCATGCATTGTAAAAATCAGCAGCAAATCAGGCCTGAGATAGCGTCCAGGCAAAACCTGGAAAATTTCTTAGTTTTGCCGGATATGAAGTGAAAAGGTGAGATGCATCACGCTTCGCGCGGTGTCTGGGCGTTCAAAAAGGGCAGTAACAGCAGTGCGGAAATCCCGGCGGCGATAGAGATAACCACAAAAAATC  >  NC_000913/3847122‑3847393

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑