Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,131,654	C→A	P73T (CCG→ACG)	flgC →	flagellar basal‑body rod protein FlgC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,131,654	0	C	A	100.0%	295.9 / NA	84	P73T (CCG→ACG)	flgC	flagellar basal‑body rod protein FlgC
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (42/42);  total (42/42)

GCGAATGCTGATAGCGTGACCGGTCCCGATGGACAGCCATATCGGGCAAAACAGGTGGTATTCCAGGTTAACGCTGCACCAGGTGCTGCGACAGGCGGCGTAAAGGTTGCCGATGTTATAGAAAGTCAGGCCCCGGACAAACTGGTTTATGAACCGGGTAATCCGCTGGCAGATGCAAAGGGCTACGTAAAAATGCCGAACGTTGATGTTGTCGGAGAGATGGTTAACACCATGTCGGCGTCACGCAGCTATCAGGCCAATGTTGAAG  >  NC_000913/1131522‑1131789                                                                                                                                     |                                                                                                                                        gCGAATGCTGATAGCGTGACCGGTCCCGATGGACAGCCATATCGGGCAAAACAGGTGGTATTCCAGGTTAACGCTGCACCAGGTGCTGCGACAGGCGGCGTAAAGGTTGCCGATGTTATAGAAAGTCAGGCCACGGACaa                                                                                                                                  >  2:901661/1‑140 (MQ=255)  cGAATGCTGATAGCGTGACCGGTCCCGATGGACAGCCATATCGGGCAAAACAGGTGGTATTCCAGGTTAACGCTGCACCAGGTGCTGCGACAGGCGGCGTAAAGGTTGCCGATGTTATAGAAAGTCAGGCCACGGACaaa                                                                                                                                 <  2:873469/140‑1 (MQ=255)    aaTGCTGATAGCGTGACCGGTCCCGATGGACAGCCATATCGGGCAAAACAGGTGGTATTCCAGGTTAACGCTGCACCAGGTGCTGCGACAGGCGGCGTAAAGGTTGCCGATGTTATAGAAAGTCAGGCCACGGACAAACt                                                                                                                               >  2:969109/1‑140 (MQ=255)    aaTGCTGATAGCGTGACCGGTCCCGATGGACAGCCATATCGGGCAAAACAGGTGGTATTCCAGGTTAACGCTGCACCAGGTGCTGCGACAGGCGGCGTAAAGGTTGCCGATGTTATAGAAAGTCAGGCCACGGACAAACt                                                                                                                               <  1:1119583/140‑1 (MQ=255)    aaTGCTGATAGCGTGACCGGTCCCGATGGACAGCCATATCGGGCAAAACAGGTGGTATTCCAGGTTAACGCTGCACCAGGTGCTGCGACAGGCGGCGTAAAGGTTGCCGATGTTATAGAAAGTCAGGCCACGGACAAACt                                                                                                                               >  1:292318/1‑140 (MQ=255)    aaTGCTGATAGCGTGACCGGTCCCGATGGACAGCCATATCGGGCAAAACAGGTGGTATTCCAGGTTAACGCTGCACCAGGTGCTGCGACAGGCGGCGTAAAGGTTGCCGATGTTATAGAAAGTCAGGCCACGGACAAACt                                                                                                                               <  1:290507/140‑1 (MQ=255)    aaTGCTGATAGCGTGACCGGTCCCGATGGACAGCCATATCGGGCAAAACAGGTGGTATTCCAGGTTAACGCTGCACCAGGTGCTGCGACAGGCGGCGTAAAGGTTGCCGATGTTATAGAAAGTCAGGCCACGGACAAACt                                                                                                                               <  1:249089/140‑1 (MQ=255)    aaTGCTGATAGCGTGACCGGTCCCGATGGACAGCCATATCGGGCAAAACAGGTGGTATTCCAGGTTAACGCTGCACCAGGTGCTGCGACAGGCGGCGTAAAGGTTGCCGATGTTAAAGAAAGTCAGGCCACGGACAAACt                                                                                                                               <  1:853429/140‑1 (MQ=255)        cTGATAGCGTGACCGGTCCCGATGGACAGCCATATCGGGCAAAACAGGTGGTATTCCAGGTTAACGCTGCACCAGGTGCTGCGACAGGCGGCGTAAAGGTTGCCGATGTTATAGAAAGTCAGGCCACGGACAAACTGGtt                                                                                                                           <  1:969109/140‑1 (MQ=255)         tGATAGCGTGACCGGTCCCGATGGACAGCCATATCGGGCAAAACAGGTGGTATTCCAGGTTAACGCTGCACCAGGTGCTGCGACAGGCGGCGTAAAGGTTGCCGATGTTATAGAAAGTCAGGCCACGGACAAACTGGttt                                                                                                                          <  2:1272581/140‑1 (MQ=255)             aGCGTGACCGGTCCCGATGGACAGCCATATCGGGCAAAACAGGTGGTATTCCAGGTTAACGCTGCACCAGGTGCTGCGACAGGCGGCGTAAAGGTTGCCGATGTTATAGAAAGTCAGGCCACGGACAAACTGGTTTATGa                                                                                                                      <  1:901661/140‑1 (MQ=255)             aGCGTGACCGGTCCCGATGGACAGCCATATCGGGCAAAACAGGTGGTATTCCAGGTTAACGCTGCACCAGGTGCTGCGACAGGCGGCGTAAAGGTTGCCGATGTTATAGAAAGTCAGGCCACGGACAAACTGGTTTATGa                                                                                                                      <  2:821888/140‑1 (MQ=255)              gCGTGACCGGTCCCGATGGACAGCCATATCGGGCAAAACAGGTGGTATTCCAGGTTAACGCTGCACCAGGTGCTGCGACAGGCGGCGTAAAGGTTGCCGATGTTATAGAAAGTCAGGCCACGGACAAACTGGTTTATGaa                                                                                                                     <  2:398920/140‑1 (MQ=255)                 tGACCGGTCCCGATGGACAGCCATATCGGGCAAAACAGGTGGTATTCCAGGTTAACGCTGCACCAGGTGCTGCGACAGGCGGCGTAAAGGTTGCCGATGTTATAGAAAGTCAGGCCACGGACAAACTGGTTTATGAACCg                                                                                                                  >  2:62032/1‑140 (MQ=255)                     cGGTCCCGATGGACAGCCATATCGGGCAAAACAGGTGGTATTCCAGGTTAACGCTGCACCAGGTGCTGCGACAGGCGGCGTAAAGGTTGCCGATGTTATAGAAAGTCAGGCCACGGACAAACTGGTTTATGAACCGGGTa                                                                                                              <  2:788811/140‑1 (MQ=255)                     cGGTCCCGATGGACAGCCATATCGGGCAAAACAGGTGGTATTCCAGGTTAACGCTGCACCAGGTGCTGCGACAGGCGGCGTAAAGGTTGCCGATGTTATAGAAAGTCAGGCCACGGACAAACTGGTTTATGAACCGGGTa                                                                                                              <  2:384939/140‑1 (MQ=255)                     cGGTCCCGATGGACAGCCATATCGAGCAAAACAGGTGGTATTCCAGGTTAACGCTGCACCAGGTGCTGCGACAGGCGGCGTAAAGGTTGCCGATGTTATAGAAAGTCAGGCCACGGACAAACTGGTTTATGAACCGGGTa                                                                                                              >  2:1171443/1‑140 (MQ=255)                       gTCCCGATGGACAGCCATATCGGGCAAAACAGGTGGTATTCCAGGTTAACGCTGCACCAGGTGCTGCGACAGGCGGCGTAAAGGTTGCCGATGTTATAGAAAGTCAGGCCACGGACAAACTGGTTTATGAACCGGGTAAt                                                                                                            >  2:94153/1‑140 (MQ=255)                       gTCCCGATGGACAGCCATATCGGGCAAAACAGGTGGTATTCCAGGTTAACGCTGCACCAGGTGCTGCGACAGGCGGCGTAAAGGTTGCCGATGTTATAGAAAGTCAGGCCACGGACAAACTGGTTTATGAACCGGGTAAt                                                                                                            >  1:129481/1‑140 (MQ=255)                         cccGATGGACAGCCATATCGGGCAAAACAGGTGGTATTCCAGGTTAACGCTGCACCAGGTGCTGCGACAGGCGGCGTAAAGGTTGCCGATGTTATAGAAAGTCAGGCCACGGACAAACTGGTTTATGAACCGGGTAATcc                                                                                                          >  2:1319875/1‑140 (MQ=255)                         cccGATGGACAGCCATATCGGGCAAAACAGGTGGTATTCCAGGTTAACGCTGCACCAGGTGCTGCGACAGGCGGCGTAAAGGTTGCCGATGTTATAGAAAGTCAGGCCACGGACAAACTGGTTTATGAACCGGGTAATcc                                                                                                          <  1:575734/140‑1 (MQ=255)                              tGGACAGCCATATCGGGCAAAACAGGTGGTATTCCAGGTTAACGCTGCACCAGGTGCTGCGACAGGCGGCGTAAAGGTTGCCGATGTTATAGAAAGTCAGGCCACGGACAAACTGGTTTATGAACCGGGTAATCCGCTgg                                                                                                     <  1:288188/140‑1 (MQ=255)                                 aCAGCCATATCGGGCAAAACAGGTGGTATTCCAGGTTAACGCTGCACCAGGTGCTGCGACAGGCGGCGTAAAGGTTGCCGATGTTATAGAAAGTCAGGCCACGGACAAACTGGTTTATGAACCGGGTAATCCg                                                                                                         >  2:1354579/1‑133 (MQ=255)                                 aCAGCCATATCGGGCAAAACAGGTGGTATTCCAGGTTAACGCTGCACCAGGTGCTGCGACAGGCGGCGTAAAGGTTGCCGATGTTATAGAAAGTCAGGCCACGGACAAACTGGTTTATGAACCGGGTAATCCg                                                                                                         <  1:1354579/133‑1 (MQ=255)                                  cAGCCATATCGGGCAAAACAGGTGGTATTCCAGGTTAACGCTGCACCAGGTGCTGCGACAGGCGGCGTAAAGGTTGCCGATGTTATAGAAAGTCAGGCCACGGACAAACTGGTTTATGAACCGGGTAATCCGCTGGCAGa                                                                                                 >  1:577401/1‑140 (MQ=255)                                  cAGCCATATCGGGCAAAACAGGTGGTATTCCAGGTTAACGCTGCACCAGGTGCTGCGACAGGCGGCGTAAAGGTTGCCGATGTTATAGAAAGTCAGGCCACGGACAAACTGGTTTATGAACCGGGTAATCCGCTGGCAGa                                                                                                 >  2:200685/1‑140 (MQ=255)                                  cAGCCATATCGGGCAAAACAGGTGGTATTCCAGGTTAACGCTGCACCAGGTGCTGCGACAGGCGGCGTAAAGGTTGCCGATGTTATAGAAAGTCAGGCCACGGACAAACTGGTTTATGAACCGGGTAATCCGCTGGCAGa                                                                                                 >  2:944186/1‑140 (MQ=255)                                     ccATATCGGGCAAAACAGGTGGTATTCCAGGTTAACGCTGCACCAGGTGCTGCGACAGGCGGCGTAAAGGTTGCCGATGTTATAGAAAGTCAGGCCACGGACAAACTGGTTTATGAACCGGGTAATCCGCTGGCAGATGc                                                                                              <  1:1319875/140‑1 (MQ=255)                                       atatCGGGCAAAACAGGTGGTATTCCAGGTTAACGCTGCACCAGGTGCTGCGACAGGCGGCGTAAAGGTTGCCGATGTTATAGAAAGTCAGGCCACGGACAAACTGGTTTATGAACCGGGTAATCCGCTGGCAGATGCaa                                                                                            <  2:1075363/140‑1 (MQ=255)                                        tatCGGGCAAAACAGGTGGTATTCCAGGTTAACGCTGCACCAGGTGCTGCGACAGGCGGCGTAAAGGTTGCCGATGTTATAGAAAGTCAGGCCACGGACAAACTGGTTTATGAACCGGGTAATCCGCTGGCAGATGCaaa                                                                                           >  2:574236/1‑140 (MQ=255)                                        tatCGGGCAAAACAGGTGGTATTCCAGGTTAACGCTGCACCAGGTGCTGCGACAGGCGGCGTAAAGGTTGCCGATGTTATAGAAAGTCAGGCCACGGACAAACTGGTTTATGAACCGGGTAATCCGCTGGCAGATGCaaa                                                                                           <  1:400730/140‑1 (MQ=255)                                          tCGGGCAAAACAGGTGGTATTCCAGGTTAACGCTGCACCAGGTGCTGCGACAGGCGGCGTAAAGGTTGCCGATGTTATAGAAAGTCAGGCCACGGACAAACTGGTTTATGAACCGGGTAATCCGCTGGCAGATGCAAAgg                                                                                         >  2:617154/1‑140 (MQ=255)                                           cGGGCAAAACAGGTGGTATTCCAGGTTAACGCTGCACCAGGTGCTGCGACAGGCGGCGTAAAGGTTGCCGATGTTATAGAAAGTCAGGCCACGGACAAACTGGTTTATGAACCGGGTAATCCGCTGGCAGATGCAAAggg                                                                                        >  1:485672/1‑140 (MQ=255)                                           cGGGCAAAACAGGTGGTATTCCAGGTTAACGCTGCACCAGGTGCTGCGACAGGCGGCGTAAAGGTTGCCGATGTTATAGAAAGTCAGGCCACGGACAAACTGGTTTATGAACCGGGTAATCCGCTGGCAGATGCAAAggg                                                                                        <  2:385889/140‑1 (MQ=255)                                           cGGGCAAAACAGGTGGTATTCCAGGTTAACGCTGCACCAGGTGCTGCGACAGGCGGCGTAAAGGTTGCCGATGTTATAGAAAGTCAGGCCACGGACAAACTGGTTTATGAACCGGGTAATCCGCTGGCAGATGCAAAggg                                                                                        <  1:1384288/140‑1 (MQ=255)                                           cGGGCAAAACAGGTGGTATTCCAGGTTAACGCTGCACCAGGTGCTGCGACAGGCGGCGTAAAGGTTGCCGATGTTATAGAAAGTCAGGCCACGGACAAACTGGTTTATGAACCGGGTAATCCGCTGGCAGATGCAAAggg                                                                                        <  2:976504/140‑1 (MQ=255)                                             ggCAAAACAGGTGGTATTCCAGGTTAACGCTGCACCAGGTGCTGCGACAGGCGGCGTAAAGGTTGCCGATGTTATAGAAAGTCAGGCCACGGACAAACTGGTTTATGAACCGGGTAATCCGCTGGCAGATGCAAAGGGCt                                                                                      >  2:946376/1‑140 (MQ=255)                                               cAAAACAGGTGGTATTCCAGGTTAACGCTGCACCAGGTGCTGCGACAGGCGGCGTAAAGGTTGCCGATGTTATAGAAAGTCAGGCCACGGACAAACTGGTTTATGAACCGGGTAATCCGCTGGCAGATGCAAAGGGCTAc                                                                                    >  1:106137/1‑140 (MQ=255)                                               cAAAACAGGTGGTATTCCAGGTTAACGCTGCACCAGGTGCTGCGACAGGCGGCGTAAAGGTTGCCGATGTTATAGAAAGTCAGGCCACGGACAAACTGGTTTATGAACCGGGTAATCCGCTGGCAGATGCAAAGGGCTAc                                                                                    >  1:1365204/1‑140 (MQ=255)                                               cAAAACAGGTGGTATTCCAGGTTAACGCTGCACCAGGTGCTGCGACAGGCGGCGTAAAGGTTGCCGATGTTATAGAAAGTCAGGCCACGGACAAACTGGTTTATGAACCGGGTAATCCGCTGGCAGATGCAAAGGGCTAc                                                                                    >  1:318950/1‑140 (MQ=255)                                                          gtATTCCAGGTTAACGCTGCACCAGGTGCTGCGACAGGCGGCGTAAAGGTTGCCGATGTTATAGAAAGTCAGGCCACGGACAAACTGGTTTATGAACCGGGTAATCCGCTGGCAGATGCAAAGGGCTACGTAAAAATGcc                                                                         <  1:1171443/140‑1 (MQ=255)                                                           tATTCCAGGTTAACGCTGCACCAGGTGCTGCGACAGGCGGCGTAAAGGTTGCCGATGTTATAGAAAGTCAGGCCACGGACAAACTGGTTTATGAACCGGGTAATCCGCTGGCAGATGCAAAGGGCTACGTAAAAATGCCg                                                                        <  1:944186/140‑1 (MQ=255)                                                               ccAGGTTAACGCTGCACCAGGTGCTGCGACAGGCGGCGTAAAGGTTGCCGATGTTATAGAAAGTCAGGCCACGGACAAACTGGTTTATGAACCGGGTAATCCGCTGGCAGATGCAAAGGGCTACGTAAAAATGCCGAAc                                                                     >  1:284705/1‑139 (MQ=255)                                                               ccAGGTTAACGCTGCACCAGGTGCTGCGACAGGCGGCGTAAAGGTTGCCGATGTTATAGAAAGTCAGGCCACGGACAAACTGGTTTATGAACCGGGTAATCCGCTGGCAGATGCAAAGGGCTACGTAAAAATGCCGAAc                                                                     <  2:284705/139‑1 (MQ=255)                                                               ccAGGTTAACGCTGCACCAGGTGCTGCGACAGGCGGCGTAAAGGTTGCCGATGTTATAGAAAGTCAGGCCACGGACAAACTGGTTTATGAACCGGGTAATCCGCTGGCAGATGCAAAGGGCTACGTAAAAATGCCGAACg                                                                    >  2:381101/1‑140 (MQ=255)                                                               ccAGGTTAACGCTGCACCAGGTGCTGCGACAGGCGGCGTAAAGGTTGCCGATGTTATAGAAAGTCAGGCCACGGACAAACTGGTTTATGAACCGGGTAATCCGCTGGCAGATGCAAAGGGCTACGTAAAAATGCCGAACg                                                                    >  1:1099114/1‑140 (MQ=255)                                                                 aGGTTAACGCTGCACCAGGTGCTGCGACAGGCGGCGTAAAGGTTGCCGATGTTATAGAAAGCCAGGCCACGGACAAACTGGTTTAGGAACCGGGAAATCCGCGGGCAGATGCAAAGGGCTACGAAAAAATGCCGAACGtg                                                                  >  2:83124/1‑139 (MQ=255)                                                                   gTTAACGCTGCCCCCGGTGCTGGCACAGGCGGCGTAAAGGTTGGCGATGTTATAGAAAGTCAGGCCACGGACAAACTGGTTTATGAACCGGGTAATCCGCTGGCAGATGCAAAGGGCTACGTAAAAATGCCGAACGTTGa                                                                <  1:83124/140‑1 (MQ=255)                                                                   gTTAACGCTGCACCAGGTGCTGCGACAGGCGGCGTAAAGGTTGCCGATGTTATAGAAAGTCAGGCCACGGACAAACTGGTTTATGAACCGGGTAATCCGCTGGCAGATGCAAAGGGCTACGTAAAAATGCCGAACGTTGa                                                                <  1:200685/140‑1 (MQ=255)                                                                   gTTAACGCTGCACCAGGTGCTGCGACAGGCGGCGTAAAGGTTGCCGATGTTATAGAAAGTCAGGCCACGGACAAACTGGTTTATGAACCGGGTAATCCGCTGGCAGATGCAAAGGGCTACGTAAAAATGCCGAACGTTGa                                                                <  2:106137/140‑1 (MQ=255)                                                                     tAACGCTGCACCAGGTGCTGCGACAGGCGGCGTAAAGGTTGCCGATGTTATAGAAAGTCAGGCCACGGACAAACTGGTTTATGAACCGGGTAATCCGCTGGCAGATGCAAAGGGCTACGTAAAAATGCCGAACGTTGAtg                                                              <  2:129481/140‑1 (MQ=255)                                                                        cGCTGCACCAGGTGCTGCGACAGGCGGCGTAAAGGTTGCCGATGTTATAGAAAGTCAGGCCACGGACAAACTGGTTTATGAACCGGGTAATCCGCTGGCAGATGCAAAGGGCTACGTAAAAATGCCGAACGTTGAtgttg                                                           <  1:827963/140‑1 (MQ=255)                                                                        cGCTGCACCAGGTGCTGCGACAGGCGGCGTAAAGGTTGCCGATGTTATAGAAAGTCAGGCCACGGACAAACTGGTTTATGAACCGGGTAATCCGCTGGCAGATGCAAAGGGCTACGTAAAAATGCCGAACGTTGAtgttg                                                           >  1:704628/1‑140 (MQ=255)                                                                          cTGCACCAGGTGCTGCGACAGGCGGCGTAAAGGTTGCCGATGTTATAGAAAGTCAGGCCACGGACAAACTGGTTTATGAACCGGGTAATCCGCTGGCAGATGCAAAGGGCTACGTAAAAATGCCGAACGTTGATGTTGTc                                                         >  1:586686/1‑140 (MQ=255)                                                                             cACCAGGTGCTGCGACAGGCGGCGTAAAGGTTGCCGATGTTATAGAAAGTCAGGCCACGGACAAACTGGTTTATGAACCGGGTAATCCGCTGGCAGATGCAAAGGGCTACGTAAAAATGCCGAACGTTGATGTTGTCGga                                                      >  1:1428115/1‑140 (MQ=255)                                                                             cACCAGGTGCTGCGACAGGCGGCGTAAAGGTTGCCGATGTTATAGAAAGTCAGGCCACGGACAAACTGGTTTATGAACCGGGTAATCCGCTGGCAGATGCAAAGGGCTACGTAAAAATGCCGAACGTTGATGTTGTCGga                                                      >  2:426309/1‑140 (MQ=255)                                                                             cACCAGGTGCTGCGACAGGCGGCGTAAAGGTTGCCGATGTTATAGAAAGTCAGGCCACGGACAAACTGGTTTATGAACCGGGTAATCCGCTGGCAGATGCAAAGGGCTACGTAAAAATGCCGAACGTTGATGTTGTCGga                                                      >  2:357837/1‑140 (MQ=255)                                                                             cACCAGGTGCTGCGACAGGCGGCGTAAAGGTTGCCGATGTTATAGAAAGTCAGGCCACGGACAAACTGGTTTATGAACCGGGTAATCCGCTGGCAGATGCAAAGGGCTACGTAAAAATGCCGAACGTTGATGTTGTCGga                                                      >  2:794538/1‑140 (MQ=255)                                                                              aCCAGGTGCTGCGACAGGCGGCGTAAAGGTTGCCGATGTTATAGAAAGTCAGGCCACGGACAAACTGGTTTATGAACCGGGTAATCCGCTGGCAGATGCAAAGGGCTACGTAAAAATGCCGAACGTTGATGTTGTCGgag                                                     <  2:292318/140‑1 (MQ=255)                                                                                cAGGTGCTGCGACAGGCGGCGTAAAGGTTGCCGATGTTATAGAAAGTCAGGCCACGGACAAACTGGTTTATGAACCGGGTAATCCGCTGGCAGATGCAAAGGGCTACGTAAAAATGCCGAACGTTGATGTTGTCGgagag                                                   <  2:318950/140‑1 (MQ=255)                                                                                        gcGACAGGCGGCGTAAAGGTTGCCGATGTTATAGAAAGTCAGGCCACGGACAAACTGGTTTATGAACCGGGTAATCCGCTGGCAGATGCAAAGGGCTACGTAAAAATGCCGAACGTTGATGTTGTCGGAGAGATGGTTaa                                           <  2:1099114/140‑1 (MQ=255)                                                                                         cGACAGGCGGCGTAAAGGTTGCCGATGTTATAGAAAGTCAGGCCACGGACAAACTGGTTTATGAACCGGGTAATCCGCTGGCAGATGCAAAGGGCTACGTAAAAATGCCGAACGTTGATGTTGTCGGAGAGATGGTTAac                                          >  1:1185850/1‑140 (MQ=255)                                                                                          gACAGGCGGCGTAAAGGTTGCCGATGTTATAGAAAGTCAGGCCACGGACAAACTGGTTTATGAACCGGGTAATCCGCTGGCAGATGCAAAGGGCTACGTAAAAATGCCGAACGTTGATGTTGTCGGAGAGATGGTTAaca                                         <  1:574236/140‑1 (MQ=255)                                                                                              ggcggcGTAAAGGTTGCCGATGTTATAGAAAGTCAGGCCACGGACAAACTGGTTTATGAACCGGGTAATCCGCTGGCAGATGCAAAGGGCTACGTAAAAATGCCGAACGTTGATGTTGTCGGAGAGATGGTTAACACCAt                                     >  2:1166169/1‑140 (MQ=255)                                                                                                        aGGTTGCCGATGTTATAGAAAGTCAGGCCACGGACAAACTGGTTTATGAACCGGGTAATCCGCTGGCAGATGCAAAGGGCTACGTAAAAATGCCGAACGTTGATGTTGTCGGAGAGATGGTTAACACCATGTCGGCGTCa                           <  2:1428115/140‑1 (MQ=255)                                                                                                              ccGATGTTATAGAAAGTCAGGCCACGGACAAACTGGTTTATGAACCGGGTAATCCGCTGGCAGATGCAAAGGGCTACGTAAAAATGCCGAACGTTGATGTTGTCGGAGAGATGGTTAACACCATGTCGGCGTCACGCAGc                     <  1:794538/140‑1 (MQ=255)                                                                                                                 aTGTTATAGAAAGTCAGGCCACGGACAAACTGGTTTATGAACCGGGTAATCCGCTGGCAGATGCAAAGGGCTACGTAAAAATGCCGAACGTTGATGTTGTCGGAGAGATGGTTAACACCATGTCGGCGTCACGCAGCTAt                  <  2:204674/140‑1 (MQ=255)                                                                                                                    ttATAGAAAGTCAGGCCACGGACAAACTGGTTTATGAACCGGGTAATCCGCTGGCAGATGCAAAGGGCTACGTAAAAATGCCGAACGTTGATGTTGTCGGAGAGATGGTTAACACCATGTCGGCGTCACGCAGCTATCAg               >  2:322700/1‑140 (MQ=255)                                                                                                                      ataGAAAGTCAGGCCACGGACAAACTGGTTTATGAACCGGGTAATCCGCTGGCAGATGCAAAGGGCTACGTAAAAATGCCGAACGTTGATGTTGTCGGAGAGATGGTTAACACCATGTCGGCGTCACGCAGCTATCAGGc             <  2:485672/140‑1 (MQ=255)                                                                                                                      ataGAAAGTCAGGCCACGGACAAACTGGTTTATGAACCGGGTAATCCGCTGGCAGATGCAAAGGGCTACGTAAAAATGCCGAACGTTGATGTTGTCGGAGAGATGGTTAACACCATGTCGGCGTCACGCAGCTATCAGGc             <  2:586686/140‑1 (MQ=255)                                                                                                                      ataGAAAGTCAGGCCACGGACAAACTGGTTTATGAACCGGGTAATCCGCTGGCAGATGCAAAGGGCTACGTAAAAATGCCGAACGTTGATGTTGTCGGAGAGATGGTTAACACCATGTCGGCGTCACGCAGCTATCAGGc             <  1:62032/140‑1 (MQ=255)                                                                                                                        aGAAAGTCAGGCCACGGACAAACTGGTTTATGAACCGGGTAATCCGCTGGCAGATGCAAAGGGCTACGTAAAAATGCCGAACGTTGATGTTGTCGGAGAGATGGTTAACACCATGTCGGCGTCACGCAGCTATCAGGCCa           <  2:577401/140‑1 (MQ=255)                                                                                                                            aGTCAGGCCACGGACAAACTGGTTTATGAACCGGGTAATCCGCTGGCAGATGCAAAGGGCTACGTAAAAATGCCGAACGTTGATGTTGTCGGAGAGATGGTTAACACCATGTCGGCGTCACGCAGCTATCAGGCCAATGt       >  1:411108/1‑140 (MQ=255)                                                                                                                            aGTCAGGCCACGGACAAACTGGTTTATGAACCGGGTAATCCGCTGGCAGATGCAAAGGGCTACGTAAAAATGCCGAACGTTGATGTTGTCGGAGAGATGGTTAACACCATGTCGGCGTCACGCAGCTATCAGGCCAATGt       >  2:1188534/1‑140 (MQ=255)                                                                                                                             gTCAGGCCACGGACAAACTGGTTTATGAACCGGGTAATCCGCTGGCAGATGCAAAGGGCTACGTAAAAATGCCGAACGTTGATGTTGTCGGAGAGATGGTTAACACCATGTCGGCGTCACGCAGCTATCAGGCCAATGtt      >  2:1199396/1‑140 (MQ=255)                                                                                                                              tCAGGCCACGGACAAACTGGTTTATGAACCGGGTAATCCGCTGGCAGATGCAAAGGGCTACGTAAAAATGCCGAACGTTGATGTTGTCGGAGAGATGGTTAACACCATGTCGGCGTCACGCAGCTATCAGGCCAATGTTg     >  1:1007857/1‑140 (MQ=255)                                                                                                                              tCAGGCCACGGACAAACTGGTTTATGAACCGGGTAATCCGCTGGCAGATGCAAAGGGCTACGTAAAAATGCCGAACGTTGATGTTGTCGGAGAGATGGTTAACACCATGTCGGCGTCACGCAGCTATCAGGCCAATGTTg     >  2:730713/1‑140 (MQ=255)                                                                                                                              tCAGGCCACGGACAAACTGGTTTATGAACCGGGTAATCCGCTGGCAGATGCAAAGGGCTACGTAAAAATGCCGAACGTTGATGTTGTCGGAGAGATGGTTAACACCATGTCGGCGTCACGCAGCTATCAGGCCAATGTTg     >  2:673734/1‑140 (MQ=255)                                                                                                                              tCAGGCCACGGACAAACTGGTTTATGAACCGGGTAATCCGCTGGCAGATGCAAAGGGCTACGTAAAAATGCCGAACGTTGATGTTGTCGGAGAGATGGTTAACACCATGTCGGCGTCACGCAGCTATCAGGCCAATGTTg     <  1:322700/140‑1 (MQ=255)                                                                                                                              tCAGGCCACGGACAAACTGGTTTATGAACCGGGTAATCCGCTGGCAGATGCAAAGGGCTACGTAAAAATGCCGAACGTTGATGTTGTCGGAGAGATGGTTAACACCATGTCGGCGTCACGCAGCTATCAGGCCAATGTTg     >  1:566001/1‑140 (MQ=255)                                                                                                                              tCAGGCCACGGACAAACTGGTTTATGAACCGGGTAATCCGCTGGCAGATGCAAAGGGCTACGTAAAAATGCCGAACGTTGATGTTGTCGGAGAGATGGTTAACACCATGTCGGCGTCACGCAGCTATCAGGCCAATGTTg     >  2:35298/1‑140 (MQ=255)                                                                                                                                aGGCCACGGACAAACTGGTTTATGAACCGGGTAATCCGCTGGCAGATGCAAAGGGCTACGTAAAAATGCCGAACGTTGATGTTGTCGGAGAGATGGTTAACACCATGTCGGCGTCACGCAGCTATCAGGCCAATGTTGaa   <  1:426309/140‑1 (MQ=255)                                                                                                                                aGGCCACGGACAAACTGGTTTATGAACCGGGTAATCCGCTGGCAGATGCAAAGGGCTACGTAAAAATGCCGAACGTTGATGTTGTCGGAGAGATGGTTAACACCATGTCGGCGTCACGCAGCTATCAGGCCAATGTTGaa   >  1:901155/1‑140 (MQ=255)                                                                                                                                 ggCCACGGACAAACTGGTTTATGAACCGGGTAATCCGCTGGCAGATGCAAAGGGCTACGTAAAAATGCCGAACGTTGATGTTGTCGGAGAGATGGTTAACACCATGTCGGCGTCACGCAGCTATCAGGCCAATGTTGAAg  >  1:570201/1‑140 (MQ=255)                                                                                                                                 ggCCACGGACAAACTGGTTTATGAACCGGGTAATCCGCTGGCAGATGCAAAGGGCTACGTAAAAATGCCGAACGTTGATGTTGTCGGAGAGATGGTTAACACCATGTCGGCGTCACGCAGCTATCAGGCCAATGTTGAAg  >  2:234856/1‑140 (MQ=255)                                                                                                                                     |                                                                                                                                        GCGAATGCTGATAGCGTGACCGGTCCCGATGGACAGCCATATCGGGCAAAACAGGTGGTATTCCAGGTTAACGCTGCACCAGGTGCTGCGACAGGCGGCGTAAAGGTTGCCGATGTTATAGAAAGTCAGGCCCCGGACAAACTGGTTTATGAACCGGGTAATCCGCTGGCAGATGCAAAGGGCTACGTAAAAATGCCGAACGTTGATGTTGTCGGAGAGATGGTTAACACCATGTCGGCGTCACGCAGCTATCAGGCCAATGTTGAAG  >  NC_000913/1131522‑1131789

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑