| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 646,747 | 0 | T | G | 23.1% | 83.7 / 16.1 | 39 | T255P (ACC→CCC) | citG | triphosphoribosyl‑dephospho‑CoA synthase |
| Reads supporting (aligned to +/- strand): ref base T (5/25); new base G (9/0); total (14/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 9.45e-06 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TTTATCAAGTGCTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAAT‑GCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGCCAGTTC > NC_000913/646611‑646885 | tttATCAAGTGCTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCg < 1:1158207/140‑1 (MQ=255) cAAGTGCTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAAT‑Gc < 1:777096/140‑1 (MQ=255) aaGTGCTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAAT‑Gcc < 1:489611/140‑1 (MQ=255) tGCTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAAT‑Gccccc > 1:134823/1‑140 (MQ=255) tGCTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTCGAAT‑Gccccc > 1:978740/1‑140 (MQ=255) gCTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAAT‑GCCCCCt > 2:1132469/1‑140 (MQ=255) cTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAAT‑GCCCCCtt > 2:693384/1‑140 (MQ=255) cTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAAT‑GCCCCCtt > 1:474689/1‑140 (MQ=255) cTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTTGGAT‑Gcccccct > 2:693563/1‑138 (MQ=255) cTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTCGAAT‑Gcccccct > 1:146359/1‑138 (MQ=255) ttAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAAT‑GCCCCCttt < 2:474689/140‑1 (MQ=255) ataatTAAATCTGTGCTAAAAACCAGATAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAAT‑GCCCCCTTTTTGc < 1:1082333/140‑1 (MQ=255) atTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTCGCAT‑GCCCCCcttttttaat > 2:757835/1‑135 (MQ=255) atTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGGCGAATGGCCCCCttttttaat > 2:734181/1‑136 (MQ=255) gtgCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTTGAAT‑GCCCCCCTTTTTtaataaaattttt > 2:997192/1‑133 (MQ=255) tgCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAAT‑GCCCCCTTTTTGCAATAATGt > 1:1033583/1‑135 (MQ=255) tgCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAAT‑GCCCCCTTTTTGCAATAATGt < 2:1033583/135‑1 (MQ=255) tgCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTCGGAT‑GCCCCCCTTTTTTTATAATttttttg > 2:43073/1‑137 (MQ=255) cTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAAT‑GCCCCCTTTTTGCAATAATGTTTGCGcc < 2:280303/140‑1 (MQ=255) aaaaaCCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAAT‑GCCCCCTTTTTGCAATAATGTTTGCGCCTc < 1:757835/140‑1 (MQ=255) aaCCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAAT‑GCCCCCTTTTTGCAATAATGTTTGCGCCTcgcg < 1:406265/140‑1 (MQ=255) aaCCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAAT‑GCCCCCTTTTTGCAATAATGTTTGCGCCTcgcg < 2:978740/140‑1 (MQ=255) ggTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTCGAAA‑AACCCCCTTTTTTAATAAATTTTTCGCCCCCCGCCGtt > 2:690137/1‑139 (MQ=255) aaGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAAT‑GCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGcc < 2:237522/140‑1 (MQ=255) aTCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAAT‑GCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAgcg < 2:146359/140‑1 (MQ=255) gTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAAT‑GCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAgg < 1:997192/140‑1 (MQ=255) gTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTCGGAT‑GCCCCCCTTTTTCAATAAATTTTTCTCCCCCCCCCGTAGGCAGCGCAgg > 2:956569/1‑140 (MQ=255) cAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTCGCAT‑GCCCCCTTTTTTTCATAATTTTTTCTCCTCGCCGCGGAGCCAGCGCACGcccccc > 2:904826/1‑140 (MQ=255) ccgccTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAAT‑GCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCAcc < 1:43073/140‑1 (MQ=255) aCTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAAT‑GCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATg < 2:134823/140‑1 (MQ=255) cTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAAT‑GCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGc < 2:781599/140‑1 (MQ=255) agaTTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAAT‑GCCCCCTTTTTGCAATAACGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAAc < 1:693384/140‑1 (MQ=255) tcgttcgATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTCTTAT‑GACCCCCTTTTTTTATAATGTTTTCGCCCCCCCGCGTAGTCAGCGCGGGCCCCCCCCCCCCCCCCCCGACGCacccctgg > 2:1057234/1‑133 (MQ=255) ttcgATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAAT‑GCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTAt < 1:904826/140‑1 (MQ=255) tGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTCGGAT‑GCCCCCCTTTTTTAATAAAATTTTTGCCCCGCGCCGTCGCCACCGCTGGCCCCCCCCCCCCCCCCCCGGGGGGACGTTGGTATTGCCGTTGGTTGcc > 2:342361/1‑140 (MQ=255) gTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAAT‑GCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCa < 1:342361/140‑1 (MQ=255) gTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGTTCGAAT‑GCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCAt < 2:736732/140‑1 (MQ=255) tCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAAT‑GCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCAt < 1:693563/140‑1 (MQ=255) aCTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAAT‑GCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGt < 1:1132469/140‑1 (MQ=255) tGCCGGAGATAATCGAGATCGGCGGGGGGTTGGAT‑GCCCCCCTTTTTCAATAATGTTTTCGCCCCCCCCCGTAGCCAGCGCCGGCCCCCCCCCCCCCCCCCCGGTGCAACGTTTGGTTCGCCCGTGGTCGCCCTcagcag > 2:357196/1‑140 (MQ=255) tGCCGGAGATAATCGAGATCGGCGGGGGGTCGGAT‑GCCCCCCTTTTTCAATAATATTTTCGCCCCCCGCTGTAGCCAGCGCCGGCCCCCCCCCCCCCCCCCCGATGCAACGGTTGGATCGCCCGTGATCGCCATCAGGAg > 2:896504/1‑140 (MQ=255) aaTCGAGATCGGCGGGGGGTCGAAT‑GCCCCCCTTTTTCAATAAATTTTTCGCCCCCCGCCGTAGCCAGCGCCGGCCCCCCCCCCCCCCCCGCGATGCCACCTTGGGATCGCCGTTGATCGCCCTCAGTAGGAGCAAggga > 2:443044/1‑138 (MQ=255) tCGAGATCGGCGGGGGGTCGGAT‑GCCCCCCTTTTTCAATAATGTTTTTGCCCCCCGCCGTAGCCAGCGCCGGCCCCCCCCCCCCCCCCCCGATGCAACGTTTGTATCGCCGCTGATCGCCATCAGTAGGAGCAAGGGATc > 2:644337/1‑140 (MQ=255) gaTCGGCGGGGGTTCGAAT‑GCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGc < 1:690137/140‑1 (MQ=255) gcggGGGTTCGAAT‑GCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCTGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGc < 1:254221/140‑1 (MQ=255) gTTCGAAT‑GCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGCCAGTTc < 1:644337/140‑1 (MQ=255) | TTTATCAAGTGCTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAAT‑GCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGCCAGTTC > NC_000913/646611‑646885 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |