Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,575,824	C→A	G413V (GGC→GTC)	gntU ←	low‑affinity gluconate transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,575,824	0	C	A	100.0%	336.7 / NA	94	G413V (GGC→GTC)	gntU	low‑affinity gluconate transporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (47/47);  total (47/47)

CGTCACAACTACCGGGCGAACAAACTTAACTCAACAGCTGGAACGCAATCATCCCAACGATGGCACCGACAGTGCCGAGGATGGTTTCCATCATGGTCCAGGTTTTCAGCGTTTCGGCTTCGGTCGCGCCGGTAAATTTACCGAACAACCAGAAACCGGCGTCGTTAACGTGGCTGACAACAATCGAACCACCAGCGATACAAATCGACAGCGCCGCCATTTGCGCACCGGAGTAGTTCAGTTGTTCAATAACCGGCATC  >  NC_000913/3575696‑3575955                                                                                                                                 |                                                                                                                                    cGTCACAACTACCGGGCGAACAAACTTAACTCAACAGCTGGAACGCAATCATCCCAACGATGGCACCGACAGTGCCGAGGATGGTTTCCATCATGGTCCAGGTTTTCAGCGTTTCGGCTTCGGTCGCGACGGTAAATTTa                                                                                                                          >  1:399995/1‑140 (MQ=255)   tCACAACTACCGGGCGAACAAACTTAACTCAACAGCTGGAACGCAATCATCCCAACGATGGCACCGACAGTGCCGAGGATGGTTTCCATCATGGTCCAGGTTTTCAGCGTTTCGGCTTCGGTCGCGACGGTAAATTTAcc                                                                                                                        >  2:1084254/1‑140 (MQ=255)    cacaACTACCGGGCGAACAAACTTAACTCAACAGCTGGAACGCAATCATCCCAACGATGGCACCGACAGTGCCGAGGATGGTTTCCATCATGGTCCAGGTTTTCAGCGTTTCGGCTTCGGTCGCGACGGTAAATTTACCg                                                                                                                       <  1:797380/140‑1 (MQ=255)    cacaACTACCGGGCGAACAAACTTAACTCAACAGCTGGAACGCAATCATCCCAACGATGGCACCGACAGTGCCGAGGATGGTTTCCATCATGGTCCAGGTTTTCAGCGTTTCGGCTTCGGTCGCGACGGTAAATTTACCg                                                                                                                       <  2:349121/140‑1 (MQ=255)       aaCTACCGGGCGAACAAACTTAACTCAACAGCTGGAACGCAATCATCCCAACGATGGCACCGACAGTGCCGAGGATGGTTTCCATCATGGTCCAGGTTTTCAGCGTTTCGGCTTCGGTCGCGACGGTAAATTTACCgtcg                                                                                                                    <  1:438915/140‑4 (MQ=255)       aaCTACCGGGCGAACAAACTTAACTCAACAGCTGGAACGCAATCATCCCAACGATGGCACCGACAGTGCCGAGGATGGTTTCCATCATGGTCCAGGTTTTCAGCGTTTCGGCTTCGGTCGCGACGGTAAATTTACCGaac                                                                                                                    <  2:68570/140‑1 (MQ=255)        aCTACCGGGCGAACAAACTTAACTCAACAGCTGGAACGCAATCATCCCAACGATGGCACCGACAGTGCCGAGGATGGTTTCCATCATGGTCCAGGTTTTCAGCGTTTCGGCTTCGGTCGCGACGGTAAATTTACCGaaca                                                                                                                   >  1:213329/1‑140 (MQ=255)             cGGGCGAACAAACTTAACTCAACAGCTGGAACGCAATCATCCCAACGATGGCACCGACAGTGCCGAGGATGGTTTCCATCATGGTCCAGGTTTTCAGCGTTTCGGCTTCGGTCGCGACGGTAAATTTACCGAACAACCAg                                                                                                              >  1:689229/1‑140 (MQ=255)                   aaCAAACTTAACTCAACAGCTGGAACGCAATCATCCCAACGATGGCACCGACAGTGCCGAGGATGGTTTCCATCATGGTCCAGGTTTTCAGCGTTTCGGCTTCGGTCGCGACGGTAAATTTACCGAACAACCAGAAACCg                                                                                                        >  1:433218/1‑140 (MQ=255)                    aCAAACTTAACTCAACAGCTGGAACGCAATCATCCCAACGATGGCACCGACAGTGCCGAGGATGGTTTCCATCATGGTCCAGGTTTTCAGCGTTTCGGCTTCGGTCGCGACGGTAAATTTACCGAACAACCAGAAACCgg                                                                                                       <  2:594753/140‑1 (MQ=255)                    aCAAACTTAACTCAACAGCTGGAACGCAATCATCCCAACGATGGCACCGACAGTGCCGAGGATGGTTTCCATCATGGTCCAGGTTTTCAGCGTTTCGGCTTCGGTCGCGACGGTAAATTTACCGAACAACCAGAAACCgg                                                                                                       <  2:1075905/140‑1 (MQ=255)                    aCAAACTTAACTCAACAGCTGGAACGCAATCATCCCAACGATGGCACCGACAGTGCCGAGGATGGTTTCCATCATGGTCCAGGTTTTCAGCGTTTCGGCTTCGGTCGCGACGGTAAATTTACCGAACAACCAGAAACCgg                                                                                                       <  2:689229/140‑1 (MQ=255)                    aCAAACTTAACTCAACAGCTGGAACGCAATCATCCCAACGATGGCACCGACAGTGCCGAGGATGGTTTCCATCATGGTCCAGGTTTTCAGCGTTTCGGCTTCGGTCGCGACGGTAAATTTACCGAACAACCAGAAACCgg                                                                                                       >  1:646774/1‑140 (MQ=255)                    aCAAACTTAACTCAACAGCTGGAACGCAATCATCCCAACGATGGCACCGACAGTGCCGAGGATGGTTTCCATCATGGTCCAGGTTTTCAGCGTTTCGGCTTCGGTCGCGACGGTAAATTTACCGAACAACCAGAAACCgg                                                                                                       >  2:203830/1‑140 (MQ=255)                            aaCTCAACAGCTGGAACGCAATCATCCCAACGATGGCACCGACAGTGCCGAGGATGGTTTCCATCATGGTCCAGGTTTTCAGCGTTTCGGCTTCGGTCGCGACGGTAAATTTACCGAACAACCAGAAACCGGCGTCGTTa                                                                                               <  2:751216/140‑1 (MQ=255)                               tCAACAGCTGGAACGCAATCATCCCAACGATGGCACCGACAGTGCCGAGGATGGTTTCCATCATGGTCCAGGTTTTCAGCGTTTCGGCTTCGGTCGCGACGGTAAATTTACCGAACAACCAGAAACCGGCGTCGTTAACg                                                                                            <  2:1140278/140‑1 (MQ=255)                               tCAACAGCTGGAACGCAATCATCCCAACGATGGCACCGACAGTGCCGAGGATGGTTTCCATCATGGTCCAGGTTTTCAGCGTTTCGGCTTCGGTCGCGACGGTAAATTTACCGAACAACCAGAAACCGGCGTCGTTAACg                                                                                            <  2:399995/140‑1 (MQ=255)                                cAACAGCTGGAACGCAATCATCCCAACGATGGCACCGACAGTGCCGAGGATGGTTTCCATCATGGTCCAGGTTTTCAGCGTTTCGGCTTCGGTCGCGACGGTAAATTTACCGAACAACCAGAAACCGGCGTCGTTAACGt                                                                                           >  2:66573/1‑140 (MQ=255)                                   cAGCTGGAACGCAATCATCCCAACGATGGCACCGACAGTGCCGAGGATGGTTTCCATCATGGTCCAGGTTTTCAGCGTTTCGGCTTCGGTCGCGACGGTAAATTTACCGAACAACCAGAAACCGGCGTCGTTAACGTGGc                                                                                        <  2:1070374/140‑1 (MQ=255)                                   cAGCTGGAACGCAATCATCCCAACGATGGCACCGACAGTGCCGAGGATGGTTTCCATCATGGTCCAGGTTTTCAGCGTTTCGGCTTCGGTCGCGACGGTAAATTTACCGAACAACCAGAAACCGGCGTCGTTAACGTGGc                                                                                        >  1:7467/1‑140 (MQ=255)                                   cAGCTGGAACGCAATCATCCCAACGATGGCACCGACAGTGCCGAGGATGGTTTCCATCATGGTCCAGGTTTTCAGCGTTTCGGCTTCGGTCGCGACGGTAAATTTACCGAACAACCAGAAACCGGCGTCGTTAACGTGGc                                                                                        >  1:997177/1‑140 (MQ=255)                                   cAGCTGGAACGCAATCATCCCAACGATGGCACCGACAGTGCCGAGGATGGTTTCCATCATGGTCCAGGTTTTCAGCGTTTCGGCTTCGGTCGCGACGGTAAATTTACCGAACAACCAGAAACCGGCGTCGTTAACGTGGc                                                                                        >  1:1070374/1‑140 (MQ=255)                                   cAGCTGGAACGCAATCATCCCAACGATGGCACCGACAGTGCCGAGGATGGTTTCCATCATGGTCCAGGTTTTCAGCGTTTCGGCTTCGGTCGCGACGGTAAATTTACCGAACAACCAGAAACCGGCGTCGTTAACGTGGc                                                                                        <  1:931512/140‑1 (MQ=255)                                      cTGGAACGCAATCATCCCAACGATGGCACCGACAGTGCCGAGGATGGTTTCCATCATGGTCCAGGTTTTCAGCGTTTCGGCTTCGGTCGCGACGGTAAATTTACCGAACAACCAGAAACCGGCGTCGTTAACGTGGCTGa                                                                                     >  1:440931/1‑140 (MQ=255)                                       tGGAACGCAATCATCCCAACGATGGCACCGACAGTGCCGAGGATGGTTTCCATCATGGTCCAGGTTTTCAGCGTTTCGGCTTCGGTCGCGACGGTAAATTTACCGAACAACCAGAAACCGGCGTCGTTAACGTGGCTGac                                                                                    <  1:66573/140‑1 (MQ=255)                                        ggAACGCAATCATCCCAACGATGGCACCGACAGTGCCGAGGATGGTTTCCATCATGGTCCAGGTTTTCAGCGTTTCGGCTTCGGTCGCGACGGTAAATTTACCGAACAACCAGAAACCGGCGTCGTTAACGTGACTGaca                                                                                   >  2:341654/1‑140 (MQ=255)                                           aCGCAATCATCCCAACGATGGCACCGACAGTGCCGAGGATGGTTTCCATCATGGTCCAGGTTTTCAGCGTTTCGGCTTCGGTCGCGACGGTAAATTTACCGAACAACCAGAAACCGGCGTCGTTAACGTGGCTGacaaca                                                                                <  2:946665/140‑1 (MQ=255)                                           aCGCAATCATCCCAACGATGGCACCGACAGTGCCGAGGATGGTTTCCATCATGGTCCAGGTTTTCAGCGTTTCGGCTTCGGTCGCGACGGTAAATTTACCGAACAACCAGAAACCGGCGTCGTTAACGTGGCTGacaaca                                                                                >  2:527370/1‑140 (MQ=255)                                           aCGCAATCATCCCAACGATGGCACCGACAGTGCCGAGGATGGTTTCCATCATGGTCCAGGTTTTCAGCGTTTCGGCTTCGGTCGCGACGGTAAATTTACCGAACAACCAGAAACCGGCGTCGTTAACGTGGCTGacaaca                                                                                <  1:527370/140‑1 (MQ=255)                                             gCAATCATCCCAACGATGGCACCGACAGTGCCGAGGATGGTTTCCATCATGGTCCAGGTTTTCAGCGTTTCGGCTTCGGTCGCGACGGTAAATTTACCGAACAACCAGAAACCGGCGTCGTTAACGTGGCTGACAACAAt                                                                              <  1:476845/140‑1 (MQ=255)                                             gCAATCATCCCAACGATGGCACCGACAGTGCCGAGGATGGTTTCCATCATGGTCCAGGTTTTCAGCGTTTCGGCTTCGGTCGCGACGGTAAATTTACCGAACAACCAGAAACCGGCGTCGTTAACGTGGCTGACAACAAt                                                                              <  2:510576/140‑1 (MQ=255)                                              cAATCATCCCAACGATGGCACCGACAGTGCCGAGGATGGTTTCCATCATGGTCCAGGTTTTCAGCGTTTCGGCTTCGGTCGCGACGGTAAATTTACCGAACAACCAGAAACCGGCGTCGTTAACGTGGCTGACAACAATc                                                                             >  2:157650/1‑140 (MQ=255)                                               aaTCATCCCAACGATGGCACCGACAGTGCCGAGGATGGTTTCCATCATGGTCCAGGTTTTCAGCGTTTCGGCTTCGGTCGCGACGGTAAATTTACCGAACAACCAGAAACCGGCGTCGTTAACGTGGCTGACAACAATCg                                                                            <  2:7467/140‑1 (MQ=255)                                                atcatcCCAACGATGGCACCGACAGTGCCGAGGATGGTTTCCATCATGGTCCAGGTTTTCAGCGTTTCGGCTTCGGTCGCGACGGTAAATTTACCGAACAACCAGAAACCGGCGTCGTTAACGTGGCTGACAACAATCGa                                                                           >  1:939321/1‑140 (MQ=255)                                                   atcCCAACGATGGCACCGACAGTGCCGAGGATGGTTTCCATCATGGTCCAGGTTTTCAGCGTTTCGGCTTCGGTCGCGACGGTAAATTTACCGAACAACCAGAAACCGGCGTCGTTAACGTGGCTGACAACAATCGAacc                                                                        <  1:1081056/140‑1 (MQ=255)                                                   atcCCAACGATGGCACCGACAGTGCCGAGGATGGTTTCCATCATGGTCCAGGTTTTCAGCGTTTCGGCTTCGGTCGCGACGGTAAATTTACCGAACAACCAGAAACCGGCGTCGTTAACGTGGCTGACAACAATCGAacc                                                                        >  1:1105701/1‑140 (MQ=255)                                                        aaCGATGGCACCGACAGTGCCGAGGATGGTTTCCATCATGGTCCAGGTTTTCAGCGTTTCGGCTTCGGTCGCGACGGTAAATTTACCGAACAACCAGAAACCGGCGTCGTTAACGTGGCTGACAACAATCGAACCACCAg                                                                   <  2:646774/140‑1 (MQ=255)                                                           gATGGCACCGACAGTGCCGAGGATGGTTTCCATCATGGTCCAGGTTTTCAGCGTTTCGGCTTCGGTCGCGACGGTAAATTTACCGAACAACCAGAAACCGGCGTCGTTAACGTGGCTGACAACAATCGAACCACCAGCGa                                                                <  2:939321/140‑1 (MQ=255)                                                             tGGCACCGACAGTGCCGAGGATGGTTTCCATCATGGTCCAGGTTTTCAGCGTTTCGGCTTCGGTCGCGACGGTAAATTTACCGAACAACCAGAAACCGGCGTCGTTAACGTGGCTGACAACAATCGAACCACCAGCGATa                                                              >  2:1070361/1‑140 (MQ=255)                                                              ggCACCGACAGTGCCGAGGATGGTTTCCATCATGGTCCAGGTTTTCAGCGTTTCGGCTTCGGTCGCGACGGTAAATTTACCGAACAACCAGAAACCGGAGTCGTTAACATGGCTGACAACAATCGAACCACCAACGATAc                                                             >  1:899232/1‑140 (MQ=255)                                                               gCACCGACAGTGCCGAGGATGGTTTCCATCATGGTCCAGGTTTTCAGCGTTTCGGCTTCGGTCGCGACGGTAAATTTACCGAACAACCAGAAACCGGCGTCGTTAACGTGGCTGACAACAATCGAACCACCAGCGATACa                                                            >  2:238294/1‑140 (MQ=255)                                                               gCACCGACAGTGCCGAGGATGGTTTCCATCATGGTCCAGGTTTTCAGCGTTTCGGCTTCGGTCGCGACGGTAAATTTACCGAACAACCAGAAACCGGCGTCGTTAACGTGGCTGACAACAATCGAACCACCAGCGATACa                                                            >  2:683691/1‑140 (MQ=255)                                                               gCACCGACAGTGCCGAGGATGGTTTCCATCATGGTCCAGGTTTTCAGCGTTTCGGCTTCGGTCGCGACGGTAAATTTACCGAACAACCAGAAACCGGCGTCGTTAACGTGGCTGACAACAATCGAACCACCAGCGATACa                                                            >  2:1026107/1‑140 (MQ=255)                                                               gCACCGACAGTGCCGAGGATGGGTTCCATCATGGTCCAGGTTTTCAGCGTTTCGGCTTCGGTCGCGACGGTAAATTTACCGAACAACCAGAAACCGGCGTCGTTAACGTGGCTGACAACAATCGAACCACCAGCGATACa                                                            >  2:302188/1‑140 (MQ=255)                                                                      cAGTGCCGAGGATGGGTTCCATCATGGTCCAGGTTTTCAGCGTTTCGGCTTCGGTCGCGACGGTAAATTTACCGAACAACCAGAAACCGGCGTCGTTAACGTGGCTGACAACAATCGAACCACCAGCGATACAAATCGAc                                                     <  1:302188/140‑1 (MQ=255)                                                                         tGCCGAGGATGGTTTCCATCATGGTCCAGGTTTTCAGCGTTTCGGCTTCGGTCGCGACGGTAAATTTACCGAACAACCAGAAACCGGCGTCGTTAACGTGGCTGACAACAATCGAACCACCAGCGATACAAATCGACAgc                                                  >  1:728358/1‑140 (MQ=255)                                                                         tGCCGAGGATGGTTTCCATCATGGTCCAGGTTTTCAGCGTTTCGGCTTCGGTCGCGACGGTAAATTTACCGAACAACCAGAAACCGGCGTCGTTAACGTGGCTGACAACAATCGAACCACCAGCGATACAAATCGACAgc                                                  >  2:393548/1‑140 (MQ=255)                                                                         tGCCGAGGATGGTTTCCATCATGGTCCAGGTTTTCAGCGTTTCGGCTTCGGTCGCGACGGTAAATTTACCGAACAACCAGAAACCGGCGTCGTTAACGTGGCTGACAACAATCGAACCACCAGCGATACAAATCGACAgc                                                  >  1:590848/1‑140 (MQ=255)                                                                            cGAGGATGGTTTCCATCATGGTCCAGGTTTTCAGCGTTTCGGCTTCGGTCGCGACGGTAAATTTACCGAACAACCAGAAACCGGCGTCGTTAACGTGGCTGACAACAATCGAACCACCAGCGATACAAATCGACAGcgcc                                               <  1:1070361/140‑1 (MQ=255)                                                                               ggATGGTTTCCATCATGGTCCAGGTTTTCAGCGTTTCGGCTTCGGTCGCGACGGTAAATTTACCGAACAACCAGAAACCGGCGTCGTTAACGTGGCTGACAACAATCGAACCACCAGCGATACAAATCGACAGCgccgcc                                            <  2:691921/140‑1 (MQ=255)                                                                                gATGGTTTCCATCATGGTCCAGGTTTTCAGCGTTTCTGCTTCGGTCGCGACGGTAAATTTACCGAACAACCAGAAACCGGCGTCGTTAACGTGGCTGACAACAATCGAACCACCAGCGATAc                                                             >  2:503748/1‑122 (MQ=255)                                                                                gATGGTTTCCATCATGGTCCAGGTTTTCAGCGTTTCTGCTTCGGTCGCGACGGTAAATTTACCGAACAACCAGAAACCGGCGTCGTTAACGTGGCTGACAACAATCGAACCACCAGCGATAc                                                             <  1:503748/122‑1 (MQ=255)                                                                                gATGGTTTCCATCATGGTCCAGGTTTTCAGCGTTTCGGCTTCGGTCGCGACGGTAAATTTACCGAACAACCAGAAACCGGCGTCGTTAACGTGGCTGACAACAATCGAACCACCAGCGATACAAATCGACAGCGCCGCCa                                           >  2:786270/1‑140 (MQ=255)                                                                                    gTTTCCATCATGGTCCAGGTTTTCAGCGTTTCGGCTTCGGTCGCGACGGTAAATTTACCGAACAACCAGAAACCGGCGTCGTTAACGTGGCTGACAACAATCGAACCACCAGCGATACAAATCGACAGCGCCGCCATTTg                                       >  2:396696/1‑140 (MQ=255)                                                                                    gTTTCCATCATGGTCCAGGTTTTCAGCGTTTCGGCTTCGGTCGCGACGGTAAATTTACCGAACAACCAGAAACCGGCGTCGTTAACGTGGCTGACAACAATCGAACCACCAGCGATACAAATCGACAGCGCCGCCATTTg                                       <  1:238294/140‑1 (MQ=255)                                                                                     tttCCATCATGGTCCAGGTTTTCAGCGTTTCGGCTTCGGTCGCGACGGTAAATTTACCGAACAACCAGAAACCGGCGTCGTTAACGTGGCTGACAACAATCGAACCACCAGCGATACAAATCGACAGCGCCGCCATTTgc                                      >  1:92108/1‑140 (MQ=255)                                                                                         catcatGGTCCAGGTTTTCAGGGTTTCAGCTTCGGTCGCGACGATAAATTTACCGAACATCCAGAAACGGGGGTCGTTAACATGGCTGACAACAATCGAACCACCAGCGATACAAATCGACAGCACCGCCATTTGCGCAc                                  <  2:899232/140‑1 (MQ=255)                                                                                         catcatGGTCCAGGTTTTCAGCGTTTCGGCTTCGGTCGCGACGGTAAATTTACCGAACAACCAGAAACCGGCGTCGTTAACGTGGCTGACAACAATCGAACCACCAGCGATACAAATCGACAGCGCCGCCATTTGCGCAc                                  <  1:203830/140‑1 (MQ=255)                                                                                         catcatGGTCCAGGTTTTCAGCGTTTCGGCTTCGGTCGCGACGGTAAATTTACCGAACAACCAGAAACCGGCGTCGTTAACGTGGCTGACAACAATCGAACCACCAGCGATACAAATCGACAGCGCCGCCATTTGCGCAc                                  <  1:157650/140‑1 (MQ=255)                                                                                          atcatGGTCCAGGTTTTCAGCGTTTCGGCTTCGGTCGCGACGGTAAATTTACCGAACAACCAGAAACCGGCGTCGTTAACGTGGCTGACAACAATCGAACCACCAGCGATACAAATCGACAGCGCCGCCATTTGCGCAcc                                 >  2:420337/1‑140 (MQ=255)                                                                                            catGGTCCAGGTTTTCAGCGTTTCGGCTTCGGTCGCGACGGTAAATTTACCGAACAACCAGAAACCGGCGTCGTTAACGTGGCTGACAACAATCGAACCACCAGCGATACAAATCGACAGCGCCGCCATTTGCGCACCgg                               <  1:786270/140‑1 (MQ=255)                                                                                            catGGTCCAGGTTTTCAGCGTTTCGGCTTCGGTCGCGACGGTAAATTTACCGAACAACCAGAAACCGGCGTCGTTAACGTGGCTGACAACAATCGAACCACCAGCGATACAAATCGACAGCGCCGCCATTTGCGCACCgg                               <  1:420337/140‑1 (MQ=255)                                                                                            catGGTCCAGGTTTTCAGCGTTTCGGCTTCGGTCGCGACGGTAAATTTACCGAACAACCAGAAACCGGCGTCGTTAACGTGGCTGACAACAATCGAACCACCAGCGATACAAATCGACAGCGCCGCCATTTGCGCACCgg                               <  1:393548/140‑1 (MQ=255)                                                                                             atGGTCCAGGTTTTCAGCGTTTCGGCTTCGGTCGCGACGGTAAATTTACCGAACAACCAGAAACCGGCGTCGTTAACGTGGCTGACAACAATCGAACCACCAGCGATACAAATCGACAGCGCCGCCATTTGCGCACCGGa                              <  1:57814/140‑1 (MQ=255)                                                                                              tGGTCCAGGTTTTCAGCGTTTCGGCTTCGGTCGCGACGGTAAATTTACCGAACAACCAGAAACCGGCGTCGTTAACGTGGCTGACAACAATCGAACCACCAGCGATACAAATCGACAGCGCCGCCATTTgcgc                                    <  2:983524/133‑1 (MQ=255)                                                                                              tGGTCCAGGTTTTCAGCGTTTCGGCTTCGGTCGCGACGGTAAATTTACCGAACAACCAGAAACCGGCGTCGTTAACGTGGCTGACAACAATCGAACCACCAGCGATACAAATCGACAGCGCCGCCATTTgcgc                                    >  1:983524/1‑133 (MQ=255)                                                                                              tGGTCCAGGTTTTCAGCGTTTCGGCTTCGGTCGCGACGGTAAATTTACCGAACAACCAGAAACCGGCGTCGTTAACGTGGCTGACAACAATCGAACCACCAGCGATACAAATCGACAGCGCCGCCATTTGCGCACCGGag                             <  2:316096/140‑1 (MQ=255)                                                                                              tGGTCCAGGTTTTCAGCGTTTCGGCTTCGGTCGCGACGGTAAATTTACCGAACAACCAGAAACCGGCGTCGTTAACGTGACTGACAACAATCGAACCACCAGCGATACAAATCGACAGCGCCGCCATTTGCGCACCGGag                             <  1:341654/140‑1 (MQ=255)                                                                                               ggTCCAGGTTTTCAGCGTTTCGGCTTCGGTCGCGACGGTAAATTTACCGAACAACCAGAAACCGGCGTCGTTAACGTGGCTGACAACAATCGAACCACCAGCGATACAAATCGACAGCGCCGCCATTTGCGCACCGGagt                            >  2:943391/1‑140 (MQ=255)                                                                                                 tCCAGGTTTTCAGCGTTTCGGCTTCGGTCGCGACGGTAAATTTACCGAACAACCAGAAACCGGCGTCGTTAACGTGGCTGACAACAATCGAACCACCAGCGATACAAATCGACAGCGCCGCCATTTGCGCACCGGagtag                          >  1:859441/1‑140 (MQ=255)                                                                                                 tCCAGGTTTTCAGCGTTTCGGCTTCGGTCGCGACGGTAAATTTACCGAACAACCAGAAACCGGCGTCGTTAACGTGGCTGACAACAATCGAACCACCAGCGATACAAATCGACAGCGCCGCCATTTGCGCACCGGagtag                          >  1:522060/1‑140 (MQ=255)                                                                                                   cAGGTTTTCAGCGTTTCGGCTTCGGTCGCGACGGTAAATTTACCGAACAACCAGAAACCGGCGTCGTTAACGTGGCTGACAACAATCGAACCACCAGCGATACAAATCGACAGCGCCGCCATTTGCGCACCGGAGTAGtt                        <  1:108335/140‑1 (MQ=255)                                                                                                   cAGGTTTTCAGCGTTTCGGCTTCGGTCGCGACGGTAAATTTACCGAACAACCAGAAACCGGCGTCGTTAACGTGGCTGACAACAATCGAACCACCAGCGATACAAATCGACAGCGCCGCCATTTGCGCACCGGAGTAGtt                        <  2:859441/140‑1 (MQ=255)                                                                                                   cAGGTTTTCAGCGTTTCGGCTTCGGTCGCGACGGTAAATTTACCGAACAACCAGAAACCGGCGTCGTTAACGTGGCTGACAACAATCGAACCACCAGCGATACAAATCGACAGCGCCGCCATTTGCGCACCGGAGTAGtt                        <  1:407967/140‑1 (MQ=255)                                                                                                   cAGGTTTTCAGCGTTTCGGCTTCGGTCGCGACGGTAAATTTACCGAACAACCAGAAACCGGCGTCGTTAACGTGGCTGACAACAATCGAACCACCAGCGATACAAATCGACAGCGCCGCCATTTGCGCACCGGAGTAGtt                        >  1:521406/1‑140 (MQ=255)                                                                                                    aGGTTTTCAGCGTTTCGGCTTCGGTCGCGACGGTAAATTTACCGAACAACCAGAAACCGGCGTCGTTAACGTGGCTGACAACAATCGAACCACCAGCGATACAAATCGACAGCGCCGCCATTTGCGCACCGGAGTAGTTc                       <  2:1105701/140‑1 (MQ=255)                                                                                                        tttCAGCGTTTCGGCTTCGGTCGCGACGGTAAATTTACCGAACAACCAGAAACCGGCGTCGTTAACGTGGCTGACAACAATCGAACCACCAGCGATACAAATCGACAGCGCCGCCATTTGCGCACCGGAGTAGTTCAgtt                   <  1:1026107/140‑1 (MQ=255)                                                                                                          tCAGCGTTTCGGCTTCGGTCGCGACGGTAAATTTACCGAACAACCAGAAACCGGCGTCGTTAACGTGGCTGACAACAATCGAACCACCAGCGATACAAATCGACAGCGCCGCCATTTGCGCACCGGAGTAGTTCAgttgt                 >  1:816273/1‑140 (MQ=255)                                                                                                           cggCGTTTCGGCTTCGGTCGCGACGGTAAATTTACCGAACAACCAGAAACCGGCGTCGTTAACGTGGCTGACAACAATCGAACCACCAGCGATACAAATCGACAGCGCCGCCATTTGCGCACCGGAGTAGTTCAgttgtt                >  1:1129640/3‑140 (MQ=255)                                                                                                           cAGCGTTTCGGCTTCGGTCGCGACGGTAAATTTACCGAACAACCAGAAACCGGCGTCGTTAACGTGGCTGACAACAATCGAACCACCAGCGATACAAATCGACAGCGCCGCCATTTGCGCACCGGAGTAGTTCAgttgtt                >  1:568043/1‑140 (MQ=255)                                                                                                           cAGCGTTTCGGCTTCGGTCGCGACGGTAAATTTACCGAACAACCAGAAACCGGCGTCGTTAACGTGGCTGACAACAATCGAACCACCAGCGATACAAATCGACAGCGCCGCCATTTGCGCACCGGAGTAGTTCAgttgtt                >  2:1073377/1‑140 (MQ=255)                                                                                                           cAGCGTTTCGGCTTCGGTCGCGACGGTAAATTTACCGAACAACCAGAAACCGGCGTCGTTAACGTGGCTGACAACAATCGAACCACCAGCGATACAAATCGACAGCGCCGCCATTTGCGCACCGGAGTAGTTCAgttgtt                >  1:128459/1‑140 (MQ=255)                                                                                                           cAGCGTTTCGGCTTCGGTCGCGACGGTAAATTTACCGAACAACCAGAAACCGGCGTCGTTAACGTGGCTGACAACAATCGAACCACCAGCGATACAAATCGACAGCGCCGCCATTTGCGCACCGGAGTAGTTCAgttgtt                >  1:124726/1‑140 (MQ=255)                                                                                                                tttCGGCTTCGGTCGCGACGGTAAATTTACCGAACAACCAGAAACCGGCGTCGTTAACGTGGCTGACAACAATCGAACCACCAGCGATACAAATCGACAGCGCCGCCATTTGCGCACCGGAGTAGTTCAGTTGTTCAATa           >  1:925219/1‑140 (MQ=255)                                                                                                                tttCGGCTTCGGTCGCGACGGTAAATTTACCGAACAACCAGAAACCGGCGTCGTTAACGTGGCTGACAACAATCGAACCACCAGCGATACAAATCGACAGCGCCGCCATTTGCGCACCGGAGTAGTTCAGTTGTTCAATa           >  1:552446/1‑140 (MQ=255)                                                                                                                tttCGGCTTCGGTCGCGACGGTAAATTTACCGAACAACCAGAAACCGGCGTCGTTAACGTGGCTGACAACAATCGAACCACCAGCGATACAAATCGACAGCGCCGCCATTTGCGCACCGGAGTAGTTCAGTTGTTCAATa           >  2:912321/1‑140 (MQ=255)                                                                                                                 ttCGGCTTCGGTCGCGACGGTAAATTTACCGAACAACCAGAAACCGGCGTCGTTAACGTGGCTGACAACAATCGAACCACCAGCGATACAAATCGACAGCGCCGCCATTTGCGCACCGGAGTAGTTCAGTTGTTCAATaa          <  1:1084254/140‑1 (MQ=255)                                                                                                                  tCGGCTTCGGTCGCGACGGTAAATTTACCGAACAACCAGAAACCGGCGTCGTTAACGTGGCTGACAACAATCGAACCACCAGCGATACAAATCGACAGCGCCGCCATTTGCGCACCGGAGTAGTTCAGTTGTTCAATAAc         >  1:379438/1‑140 (MQ=255)                                                                                                                     gCTTCGGTCGCGACGGTAAATTTACCGAACAACCAGAAACCGGCGTCGTTAACGTGGCTGATAACAATCGAACCACCAGCGATACAAATCGACAGCGCCGCCATTTGCGCACCGGAGTAGTTCAGTTGTTCAATAACCgg      <  2:811626/140‑1 (MQ=255)                                                                                                                      cTTCGGTCGCGACGGTAAATTTACCGAACAACCAGAAACCGGCGTCGTTAACGTGGCTGACAACAATCGAACCACCAGCGATACAAATCGACAGCGCCGCCATTTGCGCACCGGAGTAGTTCAGTTGTTCAATAACCGGc     <  2:92108/140‑1 (MQ=255)                                                                                                                       ttCGGTCGCGACGGTAAATTTACCGAACAACCAGAAACCGGCGTCGTTAACGTGGCTGACAACAATCGAACCACCAGCGATACAAATCGACAGCGCCGCCATTTGCGCACCGGAGTAGTTCAGTTGTTCAATAACCGGCa    <  2:433218/140‑1 (MQ=255)                                                                                                                       ttCGGTCGCGACGGTAAATTTACCGAACAACCAGAAACCGGCGTCGTTAACGTGGCTGACAACAATCGAACCACCAGCGATACAAATCGACAGCGCCGCCATTTGCGCACCGGAGTAGTTCAGTTGTTCAATAACCGGCa    <  1:683691/140‑1 (MQ=255)                                                                                                                         cGGTCGCGACGGTAAATTTACCGAACAACCAGAAACCGGCGTCGTTAACGTGGCTGACAACAATCGAACCACCAGCGATACAAATCGACAGCGCCGCCATTTGCGCACCGGAGTAGTTCAGTTGTTCAATaa          <  1:182436/132‑1 (MQ=255)                                                                                                                         cGGTCGCGACGGTAAATTTACCGAACAACCAGAAACCGGCGTCGTTAACGTGGCTGACAACAATCGAACCACCAGCGATACAAATCGACAGCGCCGCCATTTGCGCACCGGAGTAGTTCAGTTGTTCAATaa          >  2:182436/1‑132 (MQ=255)                                                                                                                         cGGTCGCGACGGTAAATTTACCGAACAACCAGAAACCGGCGTCGTTAACGTGGCTGACAACAATCGAACCACCAGCGATACAAATCGACAGCGCCGCCATTTGCGCACCGGAGTAGTTCAGTTGTTCAATAACCGGCATc  <  1:912321/140‑1 (MQ=255)                                                                                                                                 |                                                                                                                                    CGTCACAACTACCGGGCGAACAAACTTAACTCAACAGCTGGAACGCAATCATCCCAACGATGGCACCGACAGTGCCGAGGATGGTTTCCATCATGGTCCAGGTTTTCAGCGTTTCGGCTTCGGTCGCGCCGGTAAATTTACCGAACAACCAGAAACCGGCGTCGTTAACGTGGCTGACAACAATCGAACCACCAGCGATACAAATCGACAGCGCCGCCATTTGCGCACCGGAGTAGTTCAGTTGTTCAATAACCGGCATC  >  NC_000913/3575696‑3575955

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑