Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,958,812	G→T	A281A (GCG→GCT)	ilvC →	ketol‑acid reductoisomerase (NADP(+))

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,958,812	0	G	T	100.0%	328.0 / NA	92	A281A (GCG→GCT)	ilvC	ketol‑acid reductoisomerase (NADP(+))
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (45/47);  total (45/47)

CTTCGACAAGCTGGTGGAAGAAGGTACCGATCCAGCATACGCAGAAAAACTGATTCAGTTCGGTTGGGAAACCATCACCGAAGCACTGAAACAGGGCGGCATCACCCTGATGATGGACCGTCTCTCTAACCCGGCGAAACTGCGTGCTTATGCGCTTTCTGAACAGCTGAAAGAGATCATGGCACCCCTGTTCCAGAAACATATGGACGACATCATCTCCGGCGAATTCTCTTCCGGTATGATGGCGGACTGGGCCAACGATGATAAGA  >  NC_000913/3958677‑3958945                                                                                                                                        |                                                                                                                                      cTTCGACAAGCTGGTGGAAGAAGGTACCGATCCAGCATACGCAGAAAAACTGATTCAGTTCGGTTGGGAAACCATCACCGAAGCACTGAAACAGGGCGGCATCACCCTGATGATGGACCGTCTCTCTAACCCGGCTAAAc                                                                                                                                   >  2:364122/1‑140 (MQ=255) cTTCGACAAGCTGGTGGAAGAAGGTACCGATCCAGCATACGCAGAAAAACTGATTCAGTTCGGTTGGGAAACCATCACCGAAGCACTGAAACAGGGCGGCATCACCCTGATGATGGACCGTCTCTCTAACCCGGCTAAAc                                                                                                                                   >  1:544654/1‑140 (MQ=255)  ttCGACAAGCTGGTGGAAGAAGGTACCGATCCAGCATACGCAGAAAAACTGATTCAGTTCGGTTGGGAAACCATCACCGAAGCACTGAAACAGGGCGGCATCACCCTGATGATGGACCGTCTCTCTAACCCGGCTAAACt                                                                                                                                  <  1:364122/140‑1 (MQ=255)  ttCGACAAGCTGGTGGAAGAAGGTACCGATCCAGCATACGCAGAAAAACTGATTCAGTTCGGTTGGGAAACCATCACCGAAGCACTGAAACAGGGCGGCATCACCCTGATGATGGACCGTCTCTCTAACCCGGCTAAACt                                                                                                                                  >  2:560519/1‑140 (MQ=255)  ttCGACAAGCTGGTGGAAGAAGGTACCGATCCAGCATACGCAGAAAAACTGATTCAGTTCGGTTGGGAAACCATCACCGAAGCACTGAAACAGGGCGGCATCACCCTGATGATGGACCGTCTCTCTAACCCGGCTAAACt                                                                                                                                  >  2:81985/1‑140 (MQ=255)     gACAAGCTGGTGGAAGAAGGTACCGATCCAGCATACGCAGAAAAACTGATTCAGTTCTGTTGGGAAACCATCACCGAAGCACTGAAACAGGGCGGCATCACCCTGATGATGGACCGTCTCTCTAACCCGGCTAAACTGCg                                                                                                                               <  2:700632/140‑1 (MQ=255)     gACAAGCTGGTGGAAGAAGGTACCGATCCAGCATACGCAGAAAAACTGATTCAGTTCGGTTGGGAAACCATCACCGAAGCACTGAAACAGGGCGGCATCACCCTGATGATGGACCGTCTCTCTAACCCGGCTAAACTGCg                                                                                                                               <  1:621355/140‑1 (MQ=255)     gACAAGCTGGTGGAAGAAGGTACCGATCCAGCATACGCAGAAAAACTGATTCAGTTCGGTTGGGAAACCATCACCGAAGCACTGAAACAGGGCGGCATCACCCTGATGATGGACCGTCTCTCTAACCCGGCTAAACTGCg                                                                                                                               <  1:325016/140‑1 (MQ=255)         aGCTGGTGGAAGAAGGTACCGATCCAGCATACGCAGAAAAACTGATTCAGTTCGGTTGGGAAACCATCACCGAAGCACTGAAACAGGGCGGCATCACCCTGATGATGGACCGTCTCTCTAACCCGGCTAAACTGCGTGCt                                                                                                                           >  1:835646/1‑140 (MQ=255)           cTGGTGGAAGAAGGTACCGATCCAGCATACGCAGAAAAACTGATTCAGTTCGGTTGGGAAACCATCACCGAAGCACTGAAACAGGGCGGCATCACCCTGATGATGGACCGTCTCTCTAACCCGGCTAAACTGCGTGCTTa                                                                                                                         >  1:464483/1‑140 (MQ=255)             ggtggAAGAAGGTACCGATCCAGCATACGCAGAAAAACTGATTCAGTTCGGTTGGGAAACCATCACCGAAGCACTGAAACAGGGCGGCATCACCCTGATGATGGACCGTCTCTCTAACCCGGCTAAACTGCGTGCTTATg                                                                                                                       <  1:249414/140‑1 (MQ=255)             ggtggAAGAAGGTACCGATCCAGCATACGCAGAAAAACTGATTCAGTTCGGTTGGGAAACCATCACCGAAGCACTGAAACAGGGCGGCATCACCCTGATGATGGACCGTCTCTCTAACCCGGCTAAACTGCGTGCTTATg                                                                                                                       <  2:544654/140‑1 (MQ=255)                        gTACCGATCCAGCATACGCAGAAAAACTGATTCAGTTCGGTTGGGAAACCATCACCGAAGCACTGAAACAGGGCGGCATCACCCTGATGATGGACCGTCTCTCTAACCCGGCTAAACTGCGTGCTTATGCGCTTTCTGaa                                                                                                            >  1:736457/1‑140 (MQ=255)                        gTACCGATCCAGCATACGCAGAAAAACTGATTCAGTTCGGTTGGGAAACCATCACCGAAGCACTGAAACAGGGCGGCATCACCCTGATGATGGACCGCCTCTCTAACCCGGCTAAACTGCGTGCTTATGCGCTTTCTGaa                                                                                                            >  1:62803/1‑140 (MQ=255)                         tACCGATCCAGCATACGCAGAAAAACTGATTCAGTTCGGTTGGGAAACCATCACCGAAGCACTGAAACAGGGCGGCATCACCCTGATGATGGACCGTCTCTCTAACCCGGCTAAACTGCGTGCTTATGCGCTTTCTGAAc                                                                                                           <  2:1067039/140‑1 (MQ=255)                         tACCGATCCAGCATACGCAGAAAAACTGATTCAGTTCGGTTGGGAAACCATCACCGAAGCACTGAAACAGGGCGGCATCACCCTGATGATGGACCGTCTCTCTAACCCGGCTAAACTGCGTGCTTATGCGCTTTCTGAAc                                                                                                           <  2:1041114/140‑1 (MQ=255)                           ccGATCCAGCATACGCAGAAAAACTGATTCAGTTCGGTTGGGAAACCATCACCGAAGCACTGAAACAGGGCGGCATCACCCTGATGATGGACCGTCTCTCTAACCCGGCTAAACTGCGTGCTTATGCGCTTTCTGAACAg                                                                                                         <  2:474436/140‑1 (MQ=255)                            cGATCCAGCATACGCAGAAAAACTGATTCAGTTCGGTTGGGAAACCATCACCGAAGCACTGAAACAGGGCGGCATCACCCTGATGATGGACCGTCTCTCTAACCCGGCTAAACTGCGTGCTTATGCGCTTTCTGAACAGc                                                                                                        >  1:849226/1‑140 (MQ=255)                            cGATCCAGCATACGCAGAAAAACTGATTCAGTTCGGTTGGGAAACCATCACCGAAGCACTGAAACAGGGCGGCATCACCCTGATGATGGACCGTCTCTCTAACCCGGCTAAACTGCGTGCTTATGCGCTTTCTGAACAGc                                                                                                        <  2:849226/140‑1 (MQ=255)                            cGATCCAGCATACGCAGAAAAACTGATTCAGTTCGGTTGGGAAACCATCACCGAAGCACTGAAACAGGGCGGCATCACCCTGATGATGGACCGTCTCTCTAACCCGGCTAAACTGCGTGCTTATGCGCTTTCTGAACAGc                                                                                                        >  2:257929/1‑140 (MQ=255)                             gATCCAGCATACGCAGAAAAACTGATTCAGTTCGGTTGGGAAACCATCACCGAAGCACTGAAACAGGGCGGCATCACCCTGATGATGGACCGTCTCTCTAACCCGGCTAAACTGCGTGCTTATgc                                                                                                                      >  2:541411/1‑125 (MQ=255)                             gATCCAGCATACGCAGAAAAACTGATTCAGTTCGGTTGGGAAACCATCACCGAAGCACTGAAACAGGGCGGCATCACCCTGATGATGGACCGTCTCTCTAACCCGGCTAAACTGCGTGCTTATgc                                                                                                                      <  1:541411/125‑1 (MQ=255)                               tCCAGCATACGCAGAAAAACTGATTCAGTTCGGTTGGGAAACCATCACCGAAGCACTGAAACAGGGCGGCATCACCCTGATGATGGACCGTCTCTCTAACCCGGCTAAACTGCGTGCTTATGCGCTTTCTGa                                                                                                             <  2:808251/132‑1 (MQ=255)                               tCCAGCATACGCAGAAAAACTGATTCAGTTCGGTTGGGAAACCATCACCGAAGCACTGAAACAGGGCGGCATCACCCTGATGATGGACCGTCTCTCTAACCCGGCTAAACTGCGTGCTTATGCGCTTTCTGa                                                                                                             >  1:808251/1‑132 (MQ=255)                                 cAGCATACGCAGAAAAACTGATTCAGTTCGGTTGGGAAACCATCACCGAAGCACTGAAACAGGGCGGCATCACCCTGATGATGGACCGTCTCTCTAACCCGGCTAAACTGCGTGCTTATGCGCTTTCTGa                                                                                                             <  1:794970/130‑1 (MQ=255)                                 cAGCATACGCAGAAAAACTGATTCAGTTCGGTTGGGAAACCATCACCGAAGCACTGAAACAGGGCGGCATCACCCTGATGATGGACCGTCTCTCTAACCCGGCTAAACTGCGTGCTTATGCGCTTTCTGa                                                                                                             >  2:794970/1‑130 (MQ=255)                                  aGCATACGCAGAAAAACTGATTCAGTTCGGTTGGGAAACCATCACCGAAGCACTGAAACAGGGCGGCATCACCCTGATGATGGACCGTCTCTCTAACCCGGCTAAACTGCGTGCTTATGCGCTTTCTGAACAGCTGAAag                                                                                                  >  2:301847/1‑140 (MQ=255)                                  aGCATACGCAGAAAAACTGATTCAGTTCGGTTGGGAAACCATCACCGAAGCACTGAAACAGGGCGGCATCACCCTGATGATGGACCGTCTCTCTAACCCGGCTAAACTGCGTGCTTATGCGCTTTCTGAACAGCTGAAag                                                                                                  >  1:1030464/1‑140 (MQ=255)                                   gCATACGCAGAAAAACTGATTCAGTTCGGTTGGGAAACCATCACCGAAGCACTGAAACAGGGCGGCATCACCCTGATGATGGAACGTCTCTCTAACCCGGCTAAACTGCGTGCTTATGCGCTTTCTGAACAGCTGAAaga                                                                                                 >  1:540851/1‑140 (MQ=255)                                    cATACGCAGAAAAACTGATTCAGTTCGGTTGGGAAACCATCACCGAAGCACTGAAACAGGGCGGCATCACCCTGATGATGGACCGTCTCTCTAACCCGGCTAAACTGCGTGCTTATGCGCTTTCTGAACAGCTGAAagag                                                                                                <  1:486793/140‑1 (MQ=255)                                     aTACGCAGAAAAACTGATTCAGTTCGGTTGGGAAACCATCACCGAAGCACTGAAACAGGGCGGCATCACCCTGATGATGGACCGTCTCTCTAACCCGGCTAAACTGCGTGCTTATGCGCTTTCTGAACAGCTGAAAgaga                                                                                               >  1:261754/1‑140 (MQ=255)                                      tACGCAGAAAAACTGATTCAGTTCGGTTGGGAAACCATCACCGAAGCACTGAAACAGGGCGGCATCACCCTGATGATGGACCGTCTCTCTAACCCGGCTAAACTGCGTGCTTATGCGCTTTCTGAACAGCTGAAAGAGAt                                                                                              <  1:945936/140‑1 (MQ=255)                                        cGCAGAAAAACTGATTCAGTTCGGTTGGGAAACCATCACCGAAGCACTGAAACAGGGCGGCATCACCCTGATGATGGACCGTCTCTCTAACCCGGCTAAACTGCGTGCTTATGCGCTTTCTGAACAGCTGAAAGAGATCa                                                                                            >  1:938541/1‑140 (MQ=255)                                           aGAAAAACTGATTCAGTTCGGTTGGGAAACCATCACCGAAGCACTGAAACAGGGCGGCATCACCCTGATGATGGACCGTCTCTCTAACCCGGCTAAACTGCGTGCTTATGCGCTTTCTGAACAGCTGAAAGAGATCATgg                                                                                         <  1:560519/140‑1 (MQ=255)                                            gAAAAACTGATTCAGTTCGGTTGGGAAACCATCACCGAAGCACTGAAACAGGGCGGCATCACCCTGATGATGGACCGTCTCTCTAACCCGGCTAAACTGCGTGCTTATGCGCTTTCTGAACAGCTGAAAGAGATCATGGc                                                                                        <  1:1172738/140‑1 (MQ=255)                                                aaCTGATTCAGTTCGGTTGGGAAACCATCACCGAAGCACTGAAACAGGGCGGCATCACCCTGATGATGGACCGTCTCTCTAACCCGGCTAAACTGCGTGCTTATGCGCTTTCTGAACAGCTGAAAGAGATCATGGCAccc                                                                                    <  2:986017/140‑1 (MQ=255)                                                    gATTCAGTTCGGTTGGGAAACCATCACCGAAGCACTGAAACAGGGCGGCATCACCCTGATGATGGACCGTCTCTCTAACCCGGCTAAACTGCGTGCTTATGCGCTTTCTGAACAGCTGAAAGAGATCATGGCACCCCTGt                                                                                <  1:257929/140‑1 (MQ=255)                                                     aTTCAGTTCGGTTGGGAAACCATCACCGAAGCACTGAAACAGGGCGGCATCACCCTGATGATGGACCGTCTCTCTAACCCGGCTAAACTGCGTGCTTATGCGCTTTCTGAACAGCTGAAAGAGATCATGGCACCCCTGtt                                                                               >  2:1004771/1‑140 (MQ=255)                                                         aGTTCGGTTGGGAAACCATCACCGAAGCACTGAAACAGGGCGGCATCACCCTGATGATGGACCGTCTCTCTAACCCGGCTAAACTGCGTGCTTATGCGCTTTCTGAACAGCTGAAAGAGATCATGGCACCCCTGTTCCAg                                                                           <  2:835646/140‑1 (MQ=255)                                                           ttCGGTTGGGAAACCATCACCGAAGCACTGAAACAGGGCGGCATCACCCTGATGATGGACCGTCTCTCTAACCCGGCTAAACTGCGTGCTTATGCGCTTTCTGAACAGCTGAAAGAGATCATGGCACCCCTGTTCCAGaa                                                                         >  1:598587/1‑140 (MQ=255)                                                           ttCGGTTGGGAAACCATCACCGAAGCACTGAAACAGGGCGGCATCACCCTGATGATGGACCGTCTCTCTAACCCGGCTAAACTGCGTGCTTATGCGCTTTCTGAACAGCTGAAAGAGATCATGGCACCCCTGTTCCAGaa                                                                         >  2:53953/1‑140 (MQ=255)                                                             cGGTTGGGAAACCATCACCGAAGCACTGAAACAGGGCGGCATCACCCTGATGATGGACCGTCTCTCTAACCCGGCTAAACTGCGTGCTTATGCGCTTTCTGAACAGCTGAAAGAGATCATGGCACCCCTGTTCCAGAAAc                                                                       <  2:598587/140‑1 (MQ=255)                                                               gTTGGGAAACCATCACCGAAGCACTGAAACAGGGCGGCATCACCCTGATGATGGACCGTCTCTCTAACCCGGCTAAACTGCGTGCTTATGCGCTTTCTGAACAGCTGAAAGAGATCATGGCACCCCTGTTCCAGAAACat                                                                     >  1:517551/1‑140 (MQ=255)                                                                  gggAAACCATCACCGAAGCACTGAAACAGGGCGGCATCACCCTGATGATGGACCGTCTCTCTAACCCGGCTAAACTGCGTGCTTATGCGCTTTCTGAACAGCTGAAAGAGATCATGGCACCCCTGTTCCAGAAACATATg                                                                  <  1:81985/140‑1 (MQ=255)                                                                   ggAAACCATCACCGAAGCACTGAAACAGGGCGGCATCACCCTGATGATGGACCGTCTCTCTAACCCGGCTAAACTGCGTGCTTATGCGCTTTCTGAACAGCTGAAAGAGATCATGGCACCCCTGTTCCAGAAACATATgg                                                                 <  2:938541/140‑1 (MQ=255)                                                                     aaaCCATCACCGAAGCACTGAAACAGGGCGGCATCACCCTGATGATGGACCGTCTCTCTAACCCGGCTAAACTGCGTGCTTATGCGCTTTCTGAACAGCTGAAAGAGATCATGGCACCCCTGTTCCAGAAACATATGgac                                                               >  1:912828/1‑140 (MQ=255)                                                                     aaaCCATCACCGAAGCACTGAAACAGGGCGGCATCACCCTGATGATGGACCGTCTCTCTAACCCGGCTAAACTGCGTGCTTATGCGCTTTCTGAACAGCTGAAAGAGATCATGGCACCCCTGTTCCAGAAACATATGgac                                                               >  2:1174556/1‑140 (MQ=255)                                                                          aTCACCGAAGCACTGAAACAGGGCGGCATCGCCCTGATGATGGACCGTCTCTCTAACCCGGCTAAACTGCGTGCTTATGCGCTTTCTGAACAGCTGAAAGAGATCATGGCACCCCTGTTCCAGAAACATATGgac                                                               <  1:520705/135‑1 (MQ=255)                                                                          aTCACCGAAGCACTGAAACAGGGCGGCATCACCCTGATGATGGACCGTCTCTCTAACCCGGCTAAACTGCGTGCTTATGCGCTTTCTGAACAGCTGAAAGAGATCATGGCACCCCTGTTCCAGAAACATATGgac                                                               >  2:520705/1‑135 (MQ=255)                                                                          aTCACCGAAGCACTGAAACAGGGCGGCATCACCCTGATGATGGACCGTCTCTCTAACCCGGCTAAACTGCGTGCTTATGCGCTTTCTGAACAGCTGAAAGAGATCATGGCACCCCTGTTCCAGAAACATATGGACGAcat                                                          <  2:868642/140‑1 (MQ=255)                                                                             aCCGAAGCACTGAAACAGGGCGGCATCACCCTGATGATGGACCGTCTCTCTAACCCGGCTAAACTGCGTGCTTATGCGCTTTCTGAACAGCTGAAAGAGATCATGGCACCCCTGTTCCAGAAACATATGGACGAcatcat                                                       >  1:159531/1‑140 (MQ=255)                                                                               cGAAGCACTGAAACAGGGCGGCATCACCCTGATGATGGACCGTCTCTCTAACCCGGCTAAACTGCGTGCTTATGCGCTTTCTGAACAGCTGAAAGAGATCATGGCACCCCTGTTCCAGAAACATATGGACGACATCAtct                                                     <  1:1004771/140‑1 (MQ=255)                                                                                 aaGCACTGAAACAGGGCGGCATCACCCTGATGATGGACCGTCTCTCTAACCCGGCTAAACTGCGTGCTTATGCGCTTTCTGAACAGCTGAAAGAGATCATGGCACCCCTGTTCCAGAAACATATGGACGACATCATCTcc                                                   >  1:643558/1‑140 (MQ=255)                                                                                   gCACTGAAACAGGGCGGCATCACCCTGATGATGGACCGTCTCTCTAACCCGGCTAAACTGCGTGCTTATGCGCTTTCTGAACAGCTGAAAGAGATCATGGCACCCCTGTTCCAGAAACATATGGACGACATCATCTCCgg                                                 >  2:683581/1‑140 (MQ=255)                                                                                    cACTGAAACAGGGCGGCATCACCCTGATGATGGACCGTCTCTCTAACCCGGCTAAACTGCGTGCTTATGCGCTTTCTGAACAGCTGAAAGAGATCATGGCACCCCTGTTCCAGAAACATATGGACGACATCATCTCCGGc                                                <  1:1174556/140‑1 (MQ=255)                                                                                    cACTGAAACAGGGCGGCATCACCCTGATGATGGACCGTCTCTCTAACCCGGCTAAACTGCGTGCTTATGCGCTTTCTGAACAGCTGAAAGAGATCATGGCACCCCTGTTCCAGAAACATATGGACGACATCATCTCCGGc                                                <  2:1030464/140‑1 (MQ=255)                                                                                     aCTGAAACAGGGCGGCATCACCCTGATGATGGACCGCCTCTCTAACCCGGCTAAACTGCGTGCTTATGCGCTTTCTGAACAGCTGAAAGAGATCATGGCACCCCTGTTCCAGAAACATATGGACGACATCATCTCCGGCg                                               <  2:62803/140‑1 (MQ=255)                                                                                       tGAAACAGGGCGGCATCACCCTGATGATGGACCGTCTCTCTAACCCGGCTAAACTGCGTGCTTATGCGCTTTCTGAACAGCTGAAAGAGATCATGGCACCCCTGTTCCAGAAACATATGGACGACATCATCTCCGGCGaa                                             <  1:494554/140‑1 (MQ=255)                                                                                           aCAGGGCGGCATCACCCTGATGATGGACCGTCTCTCTAACCCGGCTAAACTGCGTGCTTATGCGCTTTCTGAACAGCTGAAAGAGATCATGGCACCCCTGTTCCAGAAACATATGGACGACATCATCTCCGGCGAATtct                                         <  2:661685/140‑1 (MQ=255)                                                                                             aGGGCGGCATCACCCTGATGATGGACCGTCTCTCTAACCCGGCTAAACTGCGTGCTTATGCGCTTTCTGAACAGCTGAAAGAGATCATGGCACCCCTGTTCCAGAAACATATGGACGACATCATCTCCGGCGAATTctct                                       <  2:159531/140‑1 (MQ=255)                                                                                              gggCGGCATCACCCTGATGATGGACCGTCTCTCTAACCCGGCTAAACTGCGTGCTTATGCGCTTTCTGAACAGCTGAAAGAGATCATGGCACCCCTGTTCCAGAAACATATGGACGACATCATCTCCGGCGAATtct                                         <  1:626289/137‑1 (MQ=255)                                                                                              gggCGGCATCACCCTGATGATGGACCGTCTCTCTAACCCGGCTAAACTGCGTGCTTATGCGCTTTCTGAACAGCTGAAAGAGATCATGGCACCCCTGTTCCAGAAACATATGGACGACATCATCTCCGGCGAATtct                                         >  2:626289/1‑137 (MQ=255)                                                                                              gggCGGCATCACCCTGATGATGGACCGTCTCTCTAACCCGGCTAAACTGCGTGCTTATGCGCTTTCTGAACAGCTGAAAGAGATCATGGCACCCCTGTTCCAGAAACATATGGACGACATCATCTCCGGCGAATTCTCtt                                      >  1:1148378/1‑140 (MQ=255)                                                                                              gggCGGCATCACCCTGATGATGGACCGTCTCTCTAACCCGGCTAAACTGCGTGCTTATGCGCTTTCTGAACAGCTGAAAGAGATCATGGCACCCCTGTTCCAGAAACATATGGACGACATCATCTCCGGCGAATTCTCtt                                      >  2:1024189/1‑140 (MQ=255)                                                                                                gcggaATCACCCTGATGATGGACCGTCTCTCTAACCCGGCTAAACTGCGTGCTTATGCGCTTTCTGAACAGCTGAAAGAGATCATGGCACCCCTGTTCCAGAAACATATGGACGACATCATCTCCGGCg                                               >  2:788310/1‑129 (MQ=255)                                                                                                gcggaATCACCCTGATGATGGACCGTCTCTCTAACCCGGCTAAACTGCGTGCTTATGCGCTTTCTGAACAGCTGAAAGAGATCATGGCACCCCTGTTCCAGAAACATATGGACGACATCATCTCCGGCg                                               <  1:788310/129‑1 (MQ=255)                                                                                                   gcATCACCCTGATGATGGACCGTCTCTCTAACCCGGCTAAACTGCGTGCTTATGCGCTTTCTGAACAGCTGAAAGAGATCATGGCACCCCTGTTCCAGAAACATATGGACGACATCATCTCCGGCGAATTCTCTTc                                     >  1:681867/1‑136 (MQ=255)                                                                                                   gcATCACCCTGATGATGGACCGTCTCTCTAACCCGGCTAAACTGCGTGCTTATGCGCTTTCTGAACAGCTGAAAGAGATCATGGCACCCCTGTTCCAGAAACATATGGACGACATCATCTCCGGCGAATTCTCTTc                                     <  2:681867/136‑1 (MQ=255)                                                                                                   gcATCACCCTGATGATGGACCGTCTCTCTAACCCGGCTAAACTGCGTGCTTATGCGCTTTCTGAACAGCTGAAAGAGATCATGGCACCCCTGTTCCAGAAACATATGGACGACATCATCTCCGGCGAATTCTCTTCCGGt                                 >  2:71291/1‑140 (MQ=255)                                                                                                    cATCACCCTGATGATGGACCGTCTCTCTAACCCGGCTAAACTGCGTGCTTATGCGCTTTCTGAACAGCTGAAAGAGATCATGGCACCCCTGTTCCAGAAACATATGGACGACATCATCTCCGGCGAATTCTCTTCCGGTa                                >  2:695287/1‑140 (MQ=255)                                                                                                    cATCACCCTGATGATGGACCGTCTCTCTAACCCGGCTAAACTGCGTGCTTATGCGCTTTCTGAACAGCTGAAAGAGATCATGGCACCCCTGTTCCAGAAACATATGGACGACATCATCTCCGGCGAATTCTCTTCCGGTa                                <  1:1094557/140‑1 (MQ=255)                                                                                                    cATCACCCTGATGATGGACCGTCTCTCTAACCCGGCTAAACTGCGTGCTTATGCGCTTTCTGAACAGCTGAAAGAGATCATGGCACCCCTGTTCCAGAAACATATGGACGACATCATCTCCGGCGAATTCTCTTCCGGTa                                >  2:1094557/1‑140 (MQ=255)                                                                                                    cATCACCCTGATGATGGACCGTCTCTCTAACCCGGCTAAACTGCGTGCTTATGCGCTTTCTGAACAGCTGAAAGAGATCATGGCACCCCTGTTCCAGAAACATATGGACGACATCATCTCCGGCGAATTCTCTTCCGGTa                                <  1:695287/140‑1 (MQ=255)                                                                                                    cATCACCCTGATGATGGACCGTCTCTCTAACCCGGCTAAACTGCGTGCTTATGCGCTTTCTGAACAGCTGAAAGAGATCATGGCACCCCTGTTCCAGAAACATATGGACGACATCATCTCCGGCGAATTCTCTTCCGGTa                                <  2:886458/140‑1 (MQ=255)                                                                                                               tgatgGACCGTCTCTCTAACCCGGCTAAACTGCGTGCTTATGCGCTTTCTGAACAGCTGAAAGAGATCATGGCACCCCTGTTCCAGAAACATATGGACGACATCATCTCCGGCGAATTCTCTTCCGGTATGATGGCGGAc                     <  1:1024189/140‑1 (MQ=255)                                                                                                                  tgGACCGTCTCTCTATCCCGGCTAAACTGCGTGCTTATGCGCTTTCTGAACAGCTGAAAGAGATCATGGCACCCCTGTTCCAGAAACATATGGACGACATCATCTCCGGCGAATTCTCTTCCGGTATGATGGCGGACTgg                  <  2:879444/140‑1 (MQ=255)                                                                                                                   ggACCGTCTCTCTAACCCGGCTAAACTGCGTGCTTATGCGCTTTCTGAACAGCTGAAAGAGATCATGGCACCCCTGTTCCAGAAACATATGGACGACATCATCTCCGGCGAATTCTCTTCCGGTATGATGGCGGACTggg                 >  1:745244/1‑140 (MQ=255)                                                                                                                   ggACCGTCTCTCTAACCCGGCTAAACTGCGTGCTTATGCGCTTTCTGAACAGCTGAAAGAGATCATGGCACCCCTGTTCCAGAAACATATGGACGACATCATCTCCGGCGAATTCTCTTCCGGTATGATGGCGGACTggg                 >  2:416646/1‑140 (MQ=255)                                                                                                                    gACCGTCTCTCTAACCCGGCTAAACTGCGTGCTTATGCGCTTTCTGAACAGCTGAAAGAGATCATGGCACCCCTGTTCCAGAAACATATGGACGACATCATCTCCGGCGAATTCTCTTCCGGTATGATGGCGGACTGGGc                <  2:464483/140‑1 (MQ=255)                                                                                                                    gACCGTCTCTCTAACCCGGCTAAACTGCGTGCTTATGCGCTTTCTGAACAGCTGAAAGAGATCATGGCACCCCTGTTCCAGAAACATATGGACGACATCATCTCCGGCGAATTCTCTTCCGGTATGATGGCGGACTGGGc                >  2:404020/1‑140 (MQ=255)                                                                                                                    gACCGTCTCTCTAACCCGGCTAAACTGCGTGCTTATGCGCTTTCTGAACAGCTGAAAGAGATCATGGCACCCCTGTTCCAGAAACATATGGACGACATCATCTCCGGCGAATTCTCTTCCGGTATGATGGCGGACTGGGc                <  1:71291/140‑1 (MQ=255)                                                                                                                    gACCGTCTCTCTAACCCGGCTAAACTGCGTGCTTATGCGCTTTCTGAACAGCTGAAAGAGATCATGGCACCCCTGTTCCAGAAACATATGGACGACATCATCTCCGGCGAATTCTCTTCCGGTATGATGGCGGACTGGGc                <  1:683581/140‑1 (MQ=255)                                                                                                                      ccGTCTCTCTAACCCGGCTAAACTGCGTGCTTATGCGCTTTCTGAACAGCTGAAAGAGATCATGGCACCCCTGTTCCAGAAACATATGGACGACATCATCTCCGGCGAATTCTCTTCCGGTATGATGGCGGACTGGGCCa              <  2:517551/140‑1 (MQ=255)                                                                                                                      ccGTCTCTCTAACCCGGCTAAACTGCGTGCTTATGCGCTTTCTGAACAGCTGAAAGAGATCATGGCACCCCTGTTCCAGAAACATATGGACGACATCATCTCCGGCGAACTCTCTTCCGGTATGATGGCGGAATGGGCCa              >  2:611273/1‑140 (MQ=255)                                                                                                                       cGTCTCTCTAACCCGGCTAAACTGCGTGCTTATGCGCTTTCTGAACAGCTGAAAGAGATCATGGCACCCCTGTTCCAGAAACATATGGACGACATCATCTCCGGCGAATTCTCTTCCGGTATGATGGCGGACTGGGCCaa             <  2:261754/140‑1 (MQ=255)                                                                                                                        gTCTCTCTAACCCGGCTAAACTGCGTGCTTATGCGCTTTCTGAACAGCTGAAAGAGATCATGGCACCCCTGTTCCAGAAACATATGGACGACATCATCTCCGGCGAATTCTCTTCCGGTATGATGGCGGACTGGGCCAAc            >  1:389021/1‑140 (MQ=255)                                                                                                                        gTCTCTCTAACCCGGCTAAACTGCGTGCTTATGCGCTTTCTGAACAGCTGAAAGAGATCATGGCACCCCTGTTCCAGAAACATATGGACGACATCATCTCCGGCGAATTCTCTTCCGGTATGATGGCGGACTGGGCCAAc            >  1:726565/1‑140 (MQ=255)                                                                                                                        gTCTCTCTAACCCGGCTAAACTGCGTGCTTATGCGCTTTCTGAACAGCTGAAAGAGATCATGGCACCCCTGTTCCAGAAACATATGGACGACATCATCTCCGGCGAATTCTCTTCCGGTATGATGGCGGACTGGGCCAAc            <  2:736457/140‑1 (MQ=255)                                                                                                                        gTCTCTCTAACCCGGCTAAACTGCGTGCTTATGCGCCTTCTGAACAGCTGAAAGAGATCATGGCACCCCTGTTCCAGAAACATATGGACGACATCATCTCCGGCGAATTCTCTTCCGGTATGATGGCGGACTGGGCCaa             <  1:599411/139‑1 (MQ=255)                                                                                                                        gTCTCTCTAACCCGGCTAAACTGCGTGCTTATGCGCCTTCTGAACAGCTGAAAGAGATCATGGCACCCCTGTTCCAGAAACATATGGACGACATCATCTCCGGCGAATTCTCTTCCGGTATGATGGCGGACTGGGCCaa             >  2:599411/1‑139 (MQ=255)                                                                                                                         tctctcTAACCCGGCTAAACTGCGTGCTTATGCGCTTTCTGAACAGCTGAAAGAGATCATGGCACCCCTGTTCCAGAAACATATGGACGACATCATCTCCGGCGAATTCTCTTCCGGTATGATGGCGGACTGGGCCAACg           >  2:863398/1‑140 (MQ=255)                                                                                                                         tctctcTAACCCGGCTAAACTGCGTGCTTATGCGCTTTCTGAACAGCTGAAAGAGATCATGGCACCCCTGTTCCAGAAACATATGGACGACATCATCTCCGGCGAATTCTCTTCCGGTATGATGGCGGACTGGGCCAACg           >  2:247949/1‑140 (MQ=255)                                                                                                                                  cccGGCTAAACTGCGTGCTTATGCGCTTTCTGAACAGCTGAAAGAGATCATGGCACCCCTGTTCCAGAAACATATGGACGACATCATCTCCGGCGAATTCTCTTCCGGTATGATGGCGGACTGGGCCAACGATGATAAGa  <  1:53953/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                      CTTCGACAAGCTGGTGGAAGAAGGTACCGATCCAGCATACGCAGAAAAACTGATTCAGTTCGGTTGGGAAACCATCACCGAAGCACTGAAACAGGGCGGCATCACCCTGATGATGGACCGTCTCTCTAACCCGGCGAAACTGCGTGCTTATGCGCTTTCTGAACAGCTGAAAGAGATCATGGCACCCCTGTTCCAGAAACATATGGACGACATCATCTCCGGCGAATTCTCTTCCGGTATGATGGCGGACTGGGCCAACGATGATAAGA  >  NC_000913/3958677‑3958945

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑