Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,376,293	C→A	A87E (GCG→GAG)	ycjR →	putative ketohexose‑3‑epimerase YcjR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,376,293	0	C	A	100.0%	142.9 / NA	42	A87E (GCG→GAG)	ycjR	putative ketohexose‑3‑epimerase YcjR
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (29/13);  total (29/13)

AAGAAGTCAAAGCGGCGATCAAAGAAACCGGTTTACCGGTGACCACCGCCTGCGGTGGCTATGACGGATGGATTGGCGACTTTATCGAAGAGCGTCGTCTTAATGGCTTAAAGCAGATCGAGCGCATTCTCGAAGCGCTGGCAGAAGTGGGCGGTAAAGGCATCGTCGTTCCGGCTGCGTGGGGCATGTTTACCTTCCGCTTACCGCCGATGACCTCGCCGCGTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACT  >  NC_000913/1376158‑1376428                                                                                                                                        |                                                                                                                                        aagaagTCAAAGCGGCGATCAAAGAAACCGGTTTACCGGTGACCACCGCCTGCGGTGGCTATGACGGATGGATTGGCGACTTTATCGAAGAGCGTCGTCTTAATGGCTTAAAGCAGATCGAGCGCATTCTCGAAGAGCTg                                                                                                                                     <  1:712300/140‑1 (MQ=255)    aagTCAAAGCGGCGATCAAAGAAACCGGTTTACCGGTGACCACCGCCTGCGGTGGCTATGACGGATGGATTGGCGACTTTATCGAAGAGCGTCGTCTTAATGGCTTAAAGCAGATCGAGCGCATTCTCGAAGAGCTGGCa                                                                                                                                  >  2:235951/1‑140 (MQ=255)      gTCAAAGCGGCGATCAAAGAAACCGGTTTACCGGTGACCACCGCCTGCGGTGGCTATGACGGATGGATTGGCGACTTTATCGAAGAGCGTCGTCTTAATGGCTTAAAGCAGATCGAGCGCATTCTCGAAGAGCTGGCAGa                                                                                                                                >  2:628190/1‑140 (MQ=255)       tCAAAGCGGCGATCAAAGAAACCGGTTTACCGGTGACCACCGCCTGCGGTGGCTATGACGGATGGATTGGCGACTTTATCGAAGAGCGTCGTCTTAATGGCTTAAAGCAGATCGAGCGCATTCTCGAAGAGCTGGCAGaa                                                                                                                               >  1:916693/1‑140 (MQ=255)           aGCGGCGATCAAAGAAACCGGTTTACCGGTGACCACCGCCTGCGGTGGCTATGACGGATGGATTGGCGACTTTATCGAAGAGCGTCGTCTTAATGGCTTAAAGCAGATCGAGCGCATTCTCGAAGAGCTGGCAGAAGTgg                                                                                                                           >  2:943741/1‑140 (MQ=255)           aGCGGCGATCAAAGAAACCGGTTTACCGGTGACCACCGCCTGCGGTGGCTATGACGGATGGATTGGCGACTTTATCGAAGAGCGTCGTCTTAATGGCTTAAAGCAGATCGAGCGCATTCTCGAAGAGCTGGCAGAAGTgg                                                                                                                           <  1:1134995/140‑1 (MQ=255)                cgATCAAAGAAACCGGTTTACCGGTGACCACCGCCTGCGGTGGCTATGACGGATGGATTGGCGACTTTATCGAAGAGCGTCGTCTTAATGGCTTAAAGCAGATCGAGCGCATTCTCGAAGAGCTGGCAGAAGTGGGCGGt                                                                                                                      <  2:1111261/140‑1 (MQ=255)                cgATCAAAGAAACCGGTTTACCGGTGACCACCGCCTGCGGTGGCTATGACGGATGGATTGGCGACTTTATCGAAGAGCGTCGTCTTAATGGCTTAAAGCAGATCGAGCGCATTCTCGAAGAGCTGGCAGAAGTGGGCGGt                                                                                                                      >  2:497578/1‑140 (MQ=255)                         aaaCCGGTTTACCGGTGACCACCGCCTGCGGTGGCTATGACGGATGGATTGGCGACTTTATCGAAGAGCGTCGTCTTAATGGCTTAAAGCAGATCGAGCGCATTCTCGAAGAGCTGGCAGAAGTgg                                                                                                                           >  2:889151/1‑126 (MQ=255)                         aaaCCGGTTTACCGGTGACCACCGCCTGCGGTGGCTATGACGGATGGATTGGCGACTTTATCGAAGAGCGTCGTCTTAATGGCTTAAAGCAGATCGAGCGCATTCTCGAAGAGCTGGCAGAAGTgg                                                                                                                           <  1:889151/126‑1 (MQ=255)                         aaaCCGGTTTACCGGTGACCACCGCCTGCGGTGGCTATGACGGATGGATTGGCGACTTTATCGAAGAGCGTCGTCTTAATGGCTTAAAGCAGATCGAGCGCATTCTCGAAGAGCTGGCAGAAGTGGGCGGTAAAGGCAtc                                                                                                             <  2:799966/140‑1 (MQ=255)                             cGGTTTACCGGTGACCACCGCCTGCGGTGGCTATGACGGATGGATTGGCGACTTTATCGAAGAGCGTCGTCTTAATGGCTTAAAGCAGATCGAGCGCATTCTCGAAGAGCTGGCAGAAGTGGGCGGTAAAGGCAtcgtcg                                                                                                         >  2:143927/1‑140 (MQ=255)                                           ccaccGCCTGCGGTGGCTATGACGGATGGATTGGCGACTTTATCGAAGAGCGTCGTCTTAATGGCTTAAAGCAGATCGAGCGCATTCTCGAAGAGCTGGCAGAAGTGGGCGGTAAAGGCATCGTCGTTCCGGCTGCGTgg                                                                                           <  2:498325/140‑1 (MQ=255)                                           ccaccGCCTGCGGTGGCTATGACGGATGGATTGGCGACTTTATCGAAGAGCGTCGTCTTAATGGCTTAAAGCAGATCGAGCGCATTCTCGAAGAGCTGGCAGAAGTGGGCGGTAAAGGCATCGTCGTTCCGGCTGCGTgg                                                                                           <  1:818502/140‑1 (MQ=255)                                            caccGCCTGCGGTGGCTATGACGGATGGATTGGCGACTTTATCGAAGAGCGTCGTCTTAATGGCTTAAAGCAGATCGAGCGCATTCTCGAAGAGCTGGCAGAAGTGGGCGGTAAAGGCATCGTCGTTCCGGCTGCGTggg                                                                                          <  1:143927/140‑1 (MQ=255)                                               cGCCTGCGGTGGCTATGACGGATGGATTGGCGACTTTATCGAAGAGCGTCGTCTTAATGGCTTAAAGCAGATCGAGCGCATTCTCGAAGAGCTGGCAGAAGTGGGCGGTAAAGGCATCGTCGTTCCGGCTGCGTGGGGCa                                                                                       >  2:492412/1‑140 (MQ=255)                                                  cTGCGGTGGCTATGACGGATGGATTGGCGACTTTATCGAAGAGCGTCGTCTTAATGGCTTAAAGCAGATCGAGCGCATTCTCGAAGAGCTGGCAGAAGTGGGCGGTAAAGGCATCGTCGTTCCGGCTGCGTGGGGCATGt                                                                                    <  1:453623/140‑1 (MQ=255)                                                    gCGGTGGCTATGACGGATGGATTGGCGACTTTATCGAAGAGCGTCGTCTTAATGGCTTAAAGCAGATCGAGCGCATTCTCGAAGAGCTGGCAGAAGTGGGCGGTAAAGGCATCGTCGTTCCGGCTGCGTgggg                                                                                         >  1:845181/1‑133 (MQ=255)                                                    gCGGTGGCTATGACGGATGGATTGGCGACTTTATCGAAGAGCGTCGTCTTAATGGCTTAAAGCAGATCGAGCGCATTCTCGAAGAGCTGGCAGAAGTGGGCGGTAAAGGCATCGTCGTTCCGGCTGCGTgggg                                                                                         <  2:845181/133‑1 (MQ=255)                                                         ggCTATGACGGATGGATTGGCGACTTTATCGAAGAGCGTCGTCTTAATGGCTTAAAGCAGATCGAGCGCATTCTCGAAGAGCTGGCAGAAGTGGGCGGTAAAGGCATCGTCGTTCCGGCTGCg                                                                                              >  1:26365/1‑123 (MQ=255)                                                         ggCTATGACGGATGGATTGGCGACTTTATCGAAGAGCGTCGTCTTAATGGCTTAAAGCAGATCGAGCGCATTCTCGAAGAGCTGGCAGAAGTGGGCGGTAAAGGCATCGTCGTTCCGGCTGCg                                                                                              <  2:26365/123‑1 (MQ=255)                                                                                 tttATCGAAGAGCGTCGTCTTAATGGCTTAAAGCAGTTCGAGCGCATTCTCGAAGAGCTGGCAGAAGTGGGCGGTAAAGGCATCGTCGTTCCGGCTGCGTGGGGCATGTTTACCTTCCGCTTACCGCCGATGACCTcgcc                                                     >  1:163189/1‑140 (MQ=255)                                                                                   tATCGAAGAGCGTCGTCTTAATGGCTTAAAGCAGATCGAGCGCATTCTCGAAGAGCTGGAAGAAGTGGGCGGTAAAGGCATCGTCGTTCCGGCTGCGTGGGGCATGTTTCCCTTCCGCTTACCGCCGATGACCTcgccgc                                                   <  1:943741/140‑1 (MQ=255)                                                                                         ggagCGTTGTTTTAGTGGTTTAAAGCATTTCGAGCGCTTGCTCGAGGAGCGGGCAGAGGTGGGCGTAAAAGGCTTCGCCGTCCGGGCTGCGTGGGGGTGGTTTCCCTTCCGCTTACCGCCGATGACCTCGCCGCGTAGcc                                             <  2:163189/139‑1 (MQ=255)                                                                                         agagCGTCGTCTTAATGGCTTAAAGCAGATCGAGCGCATTCTCGAAGAGCTGGCAGAAGTGGGCGGTAAAGGCATCGTCGTTCCGGCTGCGTGGGGCATGTTTACCTTCCGCTTACCGCCGATGACCTCGCCGCGCAGcc                                             >  2:457948/1‑140 (MQ=255)                                                                                          gagCGTCGTCTTAATGGCTTAAAGCAGATCGAGCGCATTCTCGAAGAGCTGGCAGAAGTGGGCGGTAAAGGCATCGTCGTTCCGGCTGCGTGGGGCATGTTTACCTTCCGCTTACCGCCGATGACCTCGCCGCGTAGCCt                                            >  2:750896/1‑140 (MQ=255)                                                                                             cgtcgtCTTAATGGCTTAAAGCAGATCGAGCGCATTCTCGAAGAGCTGGCAGAAGTGGGCGGTAAAGGCATCGTCGTTCCGGCTGCGTGGGGCATGTTTACCTTCCGCTTACCGCCGATGACCTcgccgc                                                   >  2:1101813/1‑130 (MQ=255)                                                                                             cgtcgtCTTAATGGCTTAAAGCAGATCGAGCGCATTCTCGAAGAGCTGGCAGAAGTGGGCGGTAAAGGCATCGTCGTTCCGGCTGCGTGGGGCATGTTTACCTTCCGCTTACCGCCGATGACCTcgccgc                                                   <  1:1101813/130‑1 (MQ=255)                                                                                             cgtcgtCTTAATGGCTTAAAGCAGATCGAGCGCATTCTCGAAGAGCTGGCAGAAGTGGGCGGTAAAGGCATCGTCGTTCCGGCTGCGTGGGGCATGTTTACCTTCCGCTTACCGCCGATGACCTCGCCGCGTAGCCTGGa                                         >  1:795172/1‑140 (MQ=255)                                                                                                  tcTTAATGGCTTAAAGCAGATCGAGCGCATTCTCGAAGAGCTGGCAGAAGTGGGCGGTAAAGGCATCGTCGTTCCGGCTGCGTGGGGCATGTTTACCTTCCGCTTACCGCCGATGACCTCGCCGCGTAGCCTGGACGGCg                                    >  1:106256/1‑140 (MQ=255)                                                                                                  tcTTAATGGCTTAAAGCAGATCGAGCGCATTCTCGAAGAGCTGGCAGAAGTGGGCGGTAAAGGCATCGTCGTTCCGGCTGCGTGGGGCATGTTTACCTTCCGCTTACCGCCGATGACCTCGCCGCGTAGCCTGGACGGCg                                    >  1:882721/1‑140 (MQ=255)                                                                                                       aTGGCTTAAAGCAGATCGAGCGCATTCTCGAAGAGCTGGCAGAAGTGGGCGGTAAAGGCATCGTCGTTCCGGCTGCGTGGGGCATGTTTACCTTCCGCTTACCGCCGATGACCTCGCCGCGTAGCCTGGACGGCGACCGc                               >  1:1167290/1‑140 (MQ=255)                                                                                                          gCTTAAAGCAGAGCGGGCGCATTCTGGAGGGGCGGGGAGAAGGGCGTGAAAAAGTAATCGCCGTCCCGGCTGCGGGGGGTATTTTTCCCTTCCGATAACCGCCGATGACCTCGCCGCGTAGCCTGGACGGCGACCGCaaa                            <  2:880471/140‑1 (MQ=255)                                                                                                            ttAAAGCAGATCGAGCGCATTCTCGAAGAGCTGGCAGAAGTGGGCGGTAAAGGCATCGTCGTTCCGGCTGCGTGGGGCATGTTTACCTTCCGCTTACCGCCGATGACCTCGCCGCGTAGCCTGGACGGCGACCGCAAAAt                          >  1:1163295/1‑140 (MQ=255)                                                                                                             tAAAGCAGATCGAGCGCATTCTCGAAGAGCTGGCAGAAGTGGGCGGTAAAGGCATCGTCGTTCCGGCTGCGTGGGGCATGTTTACCTTCCGCTTACCGCCGATGACCTCGCCGCGTAGCCTGGACGGCGACCGCAAAATg                         >  2:255460/1‑140 (MQ=255)                                                                                                                    gATCGAGCGCATTCTCGAAGAGCTGGCAGAAGTGGGCGGTAAAGGCATCGTCGTTCCGGCTGCGTGGGGCATGTTTACCTTCCGCTTACCGCCGATGACCTCGCCGCGTAGCCTGGACGGCGACCGCAAAATGgtgagtg                  >  1:880471/1‑140 (MQ=255)                                                                                                                     aTCGAGCGCATTCTCGAAGAGCTGGCAGAAGTGGGCGGTAAAGGCATCGTCGTTCCGGCTGCGTGGGGCATGTTTACCTTCCGCTTACCGCCGATGACCTCGCCGCGTAGCCTGGACGGCGACCGCAAAATGgtgagtga                 >  2:652098/1‑140 (MQ=255)                                                                                                                     aTCGAGCGCATTCTCGAAGAGCTGGCAGAAGTGGGCGGTAAAGGCATCGTCGTTCCGGCTGCGTGGGGCATGCTTACCTTCCGCTTACCGCCGATGACCTCGCCGCGTAGCCTGGACGGCGACCGCAAAATGgtgagtga                 >  2:602251/1‑140 (MQ=255)                                                                                                                         aGCGCATTCTCGAAGAGCTGGCAGAAGTGGGCGGTAAAGGCATCGTCGTTCCGGCTGCGTGGGGCATGTTTACCTTCCGCTTACCGCCGATGACCTCGCCGCGTAGCCTGGACGGCGACCGc                               >  1:941662/1‑122 (MQ=255)                                                                                                                         aGCGCATTCTCGAAGAGCTGGCAGAAGTGGGCGGTAAAGGCATCGTCGTTCCGGCTGCGTGGGGCATGTTTACCTTCCGCTTACCGCCGATGACCTCGCCGCGTAGCCTGGACGGCGACCGc                               <  2:941662/122‑1 (MQ=255)                                                                                                                          gcgcATTCTCGAAGAGCTGGCAGAAGTGGGCGGTAAAGGCATCGTCGTTCCGGCTGCGTGGGGCATGTTTACCTTCCGCTTACCGCCGATGACCTCGCCGCGTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTccc            >  1:436833/1‑140 (MQ=255)                                                                                                                            gcATTCTCGAAGAGCTGGCAGAAGTGGGCGGTAAAGGCATCGTCGTTCCGGCTGCGTGGGGCATGTTTACCTTCCGCTTACCGCCGATGACCTCGCCGCGTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTg          >  2:492981/1‑140 (MQ=255)                                                                                                                              aTTCGCGAAGAGCTGGCAGAGGGGGGCGGAAAAGGCACCGTCCTTCCGGCTGGGTGGGGTATGTTTACTTCCCGCTTACCGCCGATGACCTCGCCGCGTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTgcg        <  2:1163295/140‑1 (MQ=255)                                                                                                                               ttCTCGAAGAGCTGGCAGAAGTGGGCGGTAAAGGCATCGTCGTTCCGGCTGCGTGGGGCATGTTTACCTTCCGCTTACCGCCGATGACCTCGCCGCGTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTgcgc       >  1:154799/1‑140 (MQ=255)                                                                                                                                   cGAAGAGCTGGCAGAAGTGGGCGGTAAAGGCATCGTCGTTCCGGCTGCGTGGGGCATGTTTACCTTCCGCTTACCGCCGATGACCTCGCCGCGTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTAc   >  1:987604/1‑140 (MQ=255)                                                                                                                                    gAAGAGCTGGCAGAAGTGGGCGGTAAAGGCATCGTCGTTCCGGCTGCGTGGGGCATGTTTACCTTCCGCTTACCGCCGATGACCTCGCCGCGTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACt  >  1:727306/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                        AAGAAGTCAAAGCGGCGATCAAAGAAACCGGTTTACCGGTGACCACCGCCTGCGGTGGCTATGACGGATGGATTGGCGACTTTATCGAAGAGCGTCGTCTTAATGGCTTAAAGCAGATCGAGCGCATTCTCGAAGCGCTGGCAGAAGTGGGCGGTAAAGGCATCGTCGTTCCGGCTGCGTGGGGCATGTTTACCTTCCGCTTACCGCCGATGACCTCGCCGCGTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACT  >  NC_000913/1376158‑1376428

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑