Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,051,488	C→A	S2184* (TCG→TAG)	yeeJ →	inverse autotransporter adhesin

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,051,488	0	C	A	100.0%	236.0 / NA	66	S2184* (TCG→TAG)	yeeJ	inverse autotransporter adhesin
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (30/36);  total (30/36)

CTGATCCCTGTATTGAATGGTGTTCATCAAGCTGGTCTGAGTACCACAATACAATTCACTCGCGCAGAAGACAAAATAATGAGCGGTACAGTATCAGTCAATGGTACTGACCTACCGACAACTACATTCCCTTCGCAGGGGTTCACCGGGGCGTATTATCAGTTGAATAATGACAACTTTGCCCCAGGAAAAACGGCGGCTGATTATGAGTTTTCAAGCTCTGCCTCCTGGGTCGATGTTGATGCTACCGGTAAAGTGACATTTAAAAAT  >  NC_000913/2051355‑2051624                                                                                                                                      |                                                                                                                                         cTGATCCCTGTATTGAATGGTGTTCATCAAGCTGGTCTGAGTACCACAATACAATTCACTCGCGCAGAAGACAAAATAATCAGCGGTACAGTATCAGTCAATGGTACTGACCTACCGACAACTACATTCCCTTAGCAggg                                                                                                                                    <  2:258145/140‑1 (MQ=255)   gATCCCTGTATTGAATGGTGTTCATCAAGCTGGTCTGAGTACCACAATACAATTCACTCGCGCAGAAGACAAAATAATGAGCGGTACAGTATCAGTCAATGGTACTGACCTACCGACAACTACATTCCCTTAGCAGGGGt                                                                                                                                  <  1:404453/140‑1 (MQ=255)    aTCCCTGTATTGAATGGTGTTCATCAAGCTGGTCTGAGTACCACAATACAATTCACTCGCGCAGAAGACAAAATAATGAGCGGTACAGTATCAGTCAATGGTACTGACCTACCGACAACTACATTCCCTTAGCAGGGGtt                                                                                                                                 >  1:166342/1‑140 (MQ=255)        cTGTATTGAATGGTGTTCATCAAGCTGGTCTGAGTACCACAATACAATTCACTCGCGCAGAAGACAAAATAATGAGCGGTACAGTATCAGTCAATGGTACTGACCTACCGACAACTACATTCCCTTAGCAGGGGTTCAcc                                                                                                                             <  1:1054661/140‑1 (MQ=255)        cTGTATTGAATGGTGTTCATCAAGCTGGTCTGAGTACCACAATACAATTCACTCGCGCAGAAGACAAAATAATGAGCGGTACAGTATCAGTCAATGGTACTGACCTACCGACAACTACATTCCCTTAGCAGGGGTTCAcc                                                                                                                             <  1:890305/140‑1 (MQ=255)        cTGTATTGAATGGTGTTCATCAAGCTGGTCTGAGTACCACAATACAATTCACTCGCGCAGAAGACAAAATAATGAGCGGTACAGTATCAGTCAATGGTACTGACCTACCGACAACTACATTCCCTTAGCAGGGGTTCAcc                                                                                                                             <  2:831182/140‑1 (MQ=255)          gTATTGAATGGTGTTCATCAAGCTGGTCTGAGTACCACAATACAATTCACTCGCGCAGAAGACAAAATAATGAGCGGTACAGTATCAGTTAATGGTACTGACCTACCGACAACTACATTCCCTTAGCAGGGGTTCACCgg                                                                                                                           <  1:107516/140‑1 (MQ=255)          gTATTGAATGGTGTTCATCAAGCTGGTCTGAGTACCACAATACAATTCACTCGCGCAGAAGACAAAATAATGAGCGGTACAGTATCAGTCAATGGTACTGACCTACCGACAACTACATTCCCTTAGCAGGGGTTCACCgg                                                                                                                           <  2:136288/140‑1 (MQ=255)           tATTGAATGGTGTTCATCAAGCTGGTCTGAGTACCACAATACAATTCACTCGCGCAGAAGACAAAATAATGAGCGGTACAGTATCAGTCAATGGTACTGACCTACCGACAACTACATTCCCTTAGCAGGGGTTCACCggg                                                                                                                          <  1:805439/140‑1 (MQ=255)            aTTGAATGGTGTTCATCAAGCTGGTCTGAGTACCACAATACAATTCACTCGCGCAGAAGACAAAATAATGAGCGGTACAGTATCAGTCAATGGTACTGACCTACCGACAACTACATTCCCTTAGCAGGGGTTCACCgggg                                                                                                                         <  2:1050175/140‑1 (MQ=255)             ttGAATGGTGTTCATCAAGCTGGTCTGAGTACCACAATACAATTCACTCGCGCAGAAGACAAAATAATGAGCGGTACAGTATCAGTCAATGGTACTGACCTACCGACAACTACATTCCCTTAGCAGGGGTTCACCGGGGc                                                                                                                        <  2:875055/140‑1 (MQ=255)             ttGAATGGTGTTCATCAAGCTGGTCTGAGTACCACAATACAATTCACTCGCGCAGAAGACAAAATAATGAGCGGTACAGTATCAGTCAATGGTACTGACCTACCGACAACTACATTCCCTTAGCAGGGGTTCACCGGGGc                                                                                                                        <  2:649309/140‑1 (MQ=255)                aaTGGTGTTCATCAAGCTGGTCTGAGTACCACAATACAATTCACTCGCGCAGAAGACAAAATAATGAGCGGTACAGTATCAGTCAATGGTACTGACCTACCGACAACTACATTCCCTTAGCAGGGGTTCAcc                                                                                                                             >  2:1069200/1‑132 (MQ=255)                aaTGGTGTTCATCAAGCTGGTCTGAGTACCACAATACAATTCACTCGCGCAGAAGACAAAATAATGAGCGGTACAGTATCAGTCAATGGTACTGACCTACCGACAACTACATTCCCTTAGCAGGGGTTCAcc                                                                                                                             <  1:1069200/132‑1 (MQ=255)                aaTGGTGTTCATCAAGCTGGTCTGAGTACCACAATACAATTCACTCGCGCAGAAGACAAAATAATGAGCGGTACAGTATCAGTCAATGGTACTGACCTACCGACAACTACATTCCCTTAGCAGGGGTTCACCGGGGCGta                                                                                                                     >  1:372019/1‑140 (MQ=255)                    gtgtTCATCAAGCTGGTCTGAGTACCACAATACAATTCACTCGCGCAGAAGACAAAATAATGAGCGGTACAGTATCAGTCAATGGTACTGACCTACCGACAACTACATTCCCTTAGCAGGGGTTCACCGGGGCGtattat                                                                                                                 <  2:372019/140‑1 (MQ=255)                    gtgtTCATCAAGCTGGTCTGAGTACCACAATACAATTCACTCGCGCAGAAGACAAAATAATGAGCGGTACAGTATCAGTCAATGGTACTGACCTACCGACAACTACATTCCCCTAGCAGGGGTTCACCGGGGCGtattat                                                                                                                 <  1:545012/140‑1 (MQ=255)                     tgtTCATCAAGCTGGTCTGAGTACCACAATACAATTCACTCGCGCAGAAGACAAAATAATGAGCGGTACAGTATCAGTCAATGGTACTGACCTACCGACAACTACATTCCCTTAGCAGGGGTTCACCGGGGCGTATTATc                                                                                                                >  1:1110210/1‑140 (MQ=255)                       ttCATCAAGCTGGTCTGAGTACCACAATACAATTCACTCGCGCAGAAGACAAAATAATGAGCGGTACAGTATCAGTCAATGGTACTGACCTACCGACAACTACATTCCCTTAGCAGGGGTTCACCGGGGCGtattat                                                                                                                 >  2:568092/1‑137 (MQ=255)                       ttCATCAAGCTGGTCTGAGTACCACAATACAATTCACTCGCGCAGAAGACAAAATAATGAGCGGTACAGTATCAGTCAATGGTACTGACCTACCGACAACTACATTCCCTTAGCAGGGGTTCACCGGGGCGtattat                                                                                                                 <  1:568092/137‑1 (MQ=255)                        tcatcaAGCTGGTCTGAGTACCACAATACAATTCACTCGCGCAGAAGACAAAATAATGAGCGGTACAGTATCAGTCAATGGTACTGACCTACCGACAACTACATTCCCTTAGCAGGGGTTCACCGGGGCGTATTATCAGt                                                                                                             <  2:767262/140‑1 (MQ=255)                          atcaAGCTGGTCTGAGTACCACAATACAATTCACTCGCGCAGAAGACAAAATAATGAGCGGTACAGTATCAGTCAATGGTACTGACCTACCGACAACTACATTCCCTTAGCAGGGGTTCACCGGGGCGTATTATCAGTTg                                                                                                           >  1:452097/1‑140 (MQ=255)                             aaGCTGGTCTGAGTACCACAATACAATTCACTCGCGCAGAAGACAAAATAATGAGCGGTACAGTATCAGTCAATGGTACTGACCTACCGACAACTACATTCCCTTAGCAGGGGTTCACCGGGGCGTATTATCAGTTGaat                                                                                                        <  2:166342/140‑1 (MQ=255)                             aaGCTGGTCTGAGTACCACAATACAATTCACTCGCGCAGAAGACAAAATAATGAGCGGTACAGTATCAGTCAATGGTACTGACCTACCGACAACTACATTCCCTTAGCAGGGGTTCACCGGGGCGTATTATCAGTTGaat                                                                                                        <  2:641377/140‑1 (MQ=255)                               gCTGGTCTGAGTACCACAATACAATTCACTCGCGCAGAAGACAAAATAATGAGCGGTACAGTATCAGTCAATGGTACTGACCTACCGACAACTACATTCCCTTAGCAGGGGTTCACCGGGGCGTATTATCAGTTGaataa                                                                                                      >  1:591602/1‑140 (MQ=255)                                              acaatacaatTCACTCGCGCAGAAGACAAAATAATGAGCGGTACAGTATCAGTCAATGGTACTGACCTACCGACAACTACATTCCCTTAGCAGGGGTTCACCGGGGCGTATTATCAGTTGAATAATGACAACTTTGcccc                                                                                       <  1:884517/140‑1 (MQ=255)                                               caatacaatTCACTCGCGCAGAAGACAAAATAATGAGCGGTACAGTATCAGTCAATGGTACTGACCTACCGACAACTACATTCCCTTAGCAGGGGTTCACCGGAGCGTATTATCAGTTGAATAATGACAACTTTGCCCCa                                                                                      >  2:783095/1‑140 (MQ=255)                                                  tacaatTCACTCGCGCAGAAGACAAAATAATGAGCGGTACAGTATCAGTCAATGGTACTGACCTACCGACAACTACATTCCCTTAGCAGGGGTTCACCGGGGCGTATTATCAGTTGAATAATGACAACTTTGCCCCAGGa                                                                                   >  2:666952/1‑140 (MQ=255)                                                  tacaatTCACTCGCGCAGAAGACAAAATAATGAGCGGTACAGTATCAGTCAATGGTACTGACCTACCGACAACTACATTCCCTTAGCAGGGGTTCACCGGGGCGTATTATCAGTTGAATAATGACAACTTTGCCCCAGGa                                                                                   >  2:804291/1‑140 (MQ=255)                                                       ttCACTCGCGCAGAAGACAAAATAATGAGCGGTACAGTATCAGTCAATGGTACTGACCTACCGACAACTACATTCCCTTAGCAGGGGTTCACCGGGGCGTATTATCAGTTGAATAATGACAACTTTGCCCCAGGAAAAAc                                                                              >  2:1129499/1‑140 (MQ=255)                                                        tCACTCGCGCAGAAGACAAAATAATGAGCGGTACAGTATCAGTCAATGGTACTGACCTACCGACAACTACATTCCCTTAGCAGGGGTTCACCGGGGCGTATTATCAGTTGAATAATGACAACTTTGCCCCAGGAAAAAcg                                                                             <  2:82323/140‑1 (MQ=255)                                                          aCTCGCGCAGAAGACAAAATAATGAGCGGTACAGTATCAGTCAATGGTACTGACCTACCGACAACTACATTCCCTTAGCAGGGGTTCACCGGGGCGTATTATCAGTTGAATAATGACAACTTTGCCCCAGGAAAAAcggc                                                                           >  2:1078716/1‑140 (MQ=255)                                                          aCTCGCGCAGAAGACAAAATAATGAGCGGTACAGTATCAGTCAATGGTACTGACCTACCGACAACTACATTCCCTTAGCAGGGGTTCACCGGGGCGTATTATCAGTTGAATAATGACAACTTTGCCCCAGGAAAAAcggc                                                                           <  1:1070169/140‑1 (MQ=255)                                                                          aaaTAATGAGCGGTACAGTATCAGTCAATGGTACTGACCTACCGACAACTACATTCCCTTAGCAGGGGTTCACCGGGGCGTATTATCAGTTGAATAATGACAACTTTGCCCCAGGAAAAACGGCGGCTGATTATGAGttt                                                           >  2:650183/1‑140 (MQ=255)                                                                              aatGAGCGGTACAGTATCAGTCAATGGTACTGACCTACCGACAACTACATTCCCTTAGCAGGGGTTCACCGGGGCGTATTATCAGTTGAATAATGACAACTTTGCCCCAGGAAAAACGGCGGCTGATTATGAGTTTTCaa                                                       <  1:650183/140‑1 (MQ=255)                                                                               atGAGCGGTACAGTATCAGTCAATGGTACTGACCTACCGACAACTACATTCCCTTAGCAGGGGTTCACCGGGGCGTATTATCAGTTGAATAATGACAACTTTGCCCCAGGAAAAACGGCGGCTGATTATGAGTTTTCAAg                                                      <  1:1078716/140‑1 (MQ=255)                                                                                   gCGGTACAGTATCAGTCAATGGTACTGACCTACCGACAACTACATTCCCTTAGCAGGGGTTCACCGGGGCGTATTATCAGTTGAATAATGACAACTTTGCCCCAGGAAAAACGGCGGCTGATTATGAGTTTTCAAGctct                                                  <  1:1129499/140‑1 (MQ=255)                                                                                     ggTACAGTATCAGTCAATGGTACTGACCTACCGACAACTACATTCCCTTAGCAGGGGTTCACCGGGGCGTATTATCAGTTGAATAATGACAACTTTGCCCCAGGAAAAACGGCGGCTGATTATGAGTTTTCAAGCTCTGc                                                >  1:523295/1‑140 (MQ=255)                                                                                                 gTCAATGGTACTGACCTACCGACAACTACATTCCCTTAGCAGGGGTTCACCGGGGCGTATTATCAGTTGAATAATGACAACTTTGCCCCAGGAAAAACGGCGGCTGATTATGAGTTTTCAAGctct                                                  >  2:274918/1‑126 (MQ=255)                                                                                                 gTCAATGGTACTGACCTACCGACAACTACATTCCCTTAGCAGGGGTTCACCGGGGCGTATTATCAGTTGAATAATGACAACTTTGCCCCAGGAAAAACGGCGGCTGATTATGAGTTTTCAAGctct                                                  <  1:274918/126‑1 (MQ=255)                                                                                                 gTCAATGGTACTGACCTACCGACAACTACATTCCCTTAGCAGGGGTTCACCGGGGCGTATTATCAGTTGAATAATGACAACTTTGCCCCAGGAAAAACGGCGGCTGATTATGAGTTTTCAAGct                                                    >  1:1068949/1‑124 (MQ=255)                                                                                                 gTCAATGGTACTGACCTACCGACAACTACATTCCCTTAGCAGGGGTTCACCGGGGCGTATTATCAGTTGAATAATGACAACTTTGCCCCAGGAAAAACGGCGGCTGATTATGAGTTTTCAAGct                                                    <  2:1068949/124‑1 (MQ=255)                                                                                                 gTCAATGGTACTGACCTACCGACAACTACATTCCCTTAGCAGGGGTTCACCGGGGCGTATTATCAGTTGAATAATGACAACTTTGCCCCAGGAAAAACGGCGGCTGATTATGAGTTTTCAAGCTCTGcctcc                                            <  1:85506/132‑1 (MQ=255)                                                                                                 gTCAATGGTACTGACCTACCGACAACTACATTCCCTTAGCAGGGGTTCACCGGGGCGTATTATCAGTTGAATAATGACAACTTTGCCCCAGGAAAAACGGCGGCTGATTATGAGTTTTCAAGCTCTGcctcc                                            >  2:85506/1‑132 (MQ=255)                                                                                                 gTCAATGGTACTGACCTACCGACAACTACATTCCCTTAGCAGGGGTTCACCGGGGCGTATTATCAGTTGAATAATGACAACTTTGCCCCAGGAAAAACGGCGGCTGATTATGAGTTTTCAAGCTCTGcc                                               <  2:289799/129‑1 (MQ=255)                                                                                                 gTCAATGGTACTGACCTACCGACAACTACATTCCCTTAGCAGGGGTTCACCGGGGCGTATTATCAGTTGAATAATGACAACTTTGCCCCAGGAAAAACGGCGGCTGATTATGAGTTTTCAAGCTCTGcc                                               >  1:289799/1‑129 (MQ=255)                                                                                                 gTCAATGGTACTGACCTACCGACAACTACATTCCCTTAGCAGGGGTTCACCGGGGCGTATTATCAGTTGAATAATGACAACTTTGCCCCAGGAAAAACGGCGGCTGATTATGAGTTTTCAAGCTCTGCCTCCTGGGTCGa                                    >  1:185709/1‑140 (MQ=255)                                                                                                 gTCAATGGTACTGACCTACCGACAACTACATTCCCTTAGCAGGGGTTCACCGGGGCGTATTATCAGTTGAATAATGACAACTTTGCCCCAGGAAAAACGGCGGCTGATTATGAGTTTTCAAGCTCTGCCTCCTGGGTCGa                                    >  1:971020/1‑140 (MQ=255)                                                                                                 gTCAATGGTACTGACCTACCGACAACTACATTCCCTTAGCAGGGGTTCACCGGGGCGTATTATCAGTTGAATAATGACAACTTTGCCCCAGGAAAAACGGCGGCTGATTATGAGTTTTCAAGCTCTGCCTCCTGGGTCGa                                    >  1:981426/1‑140 (MQ=255)                                                                                                 gTCAATGGTACTGACCTACCGACAACTACATTCCCTTAGCAGGGGTTCACCGGGGCGTATTATCAGTTGAATAATGACAACTTTGCCCCAGGAAAAACGGCGGCTGATTATGAGTTTTCAAGCTCTGCCTCCTGGGTCGa                                    >  1:504518/1‑140 (MQ=255)                                                                                                 gTCAATGGTACTGACCTACCGACAACTACATTCCCTTAGCAGGGGTTCACCGGGGCGTATTATCAGTTGAATAATGACAACTTTGCCCCAGGAAAAACGGCGGCTGATTATGAGTTTTCAAGCTCTGCCTCCTGGGTCGa                                    >  1:521249/1‑140 (MQ=255)                                                                                                 gTCAATGGTACTGACCTACCGACAACTACATTCCCTTAGCAGGGGTTCACCGGGGCGTATTATCAGTTGAATAATGACAACTTTGCCCCAGGAAAAACGGCGGCTGATTATGAGTTTTCAAGCTCTGCCTCCTGGGTCGa                                    >  1:533307/1‑140 (MQ=255)                                                                                                      tGGTACTGACCTACCGACAACTACATTCCCTTAGCAGGGGTTCACCGGGGCGTATTATCAGTTGAATAATGACAACTTTGCCCCAGGAAAAACGGCGGCTGATTATGAGTTTTCAAGCTCTGCCTCCTGGGTCGATGTTg                               >  1:963214/1‑140 (MQ=255)                                                                                                         tACTGACCTACCGACAACTACATTCCCTTAGCAGGGGTTCACCGGGGCGTATTATCAGTTGAATAATGACAACTTTGCCCCAGGAAAAACGGCGGCTGATTATGAGTTTTCAAGCTCTGCCTCCTGGGTCGATGTTGATg                            >  1:785274/1‑140 (MQ=255)                                                                                                          aCTGACCTACCGACAACTACATTCCCTTAGCAGGGGTTCACCGGGGCGTATTATCAGTTGAATAATGACAACTTTGCCCCAGGAAAAACGGCGGCTGATTATGAGTTTTCAAGCTCTGCCTCCTGGGTCGATGTTGATGc                           <  1:666952/140‑1 (MQ=255)                                                                                                            tGACCTACCGACAACTACATTCCCTTAGCAGGGGTTCACCGGGGCGTATTATCAGTTGAATAATGACAACTTTGCCCCAGGAAAAACGGCGGCTGATTATGAGTTTTCAAGCTCTGCCTCCTGGGt                                       >  2:208037/1‑126 (MQ=255)                                                                                                            tGACCTACCGACAACTACATTCCCTTAGCAGGGGTTCACCGGGGCGTATTATCAGTTGAATAATGACAACTTTGCCCCAGGAAAAACGGCGGCTGATTATGAGTTTTCAAGCTCTGCCTCCTGGGt                                       <  1:208037/126‑1 (MQ=255)                                                                                                            tGACCTACCGACAACTACATTCCCTTAGCAGGGGTTCACCGGGGCGTATTATCAGTTGAATAATGACAACTTTGCCCCAGGAAAAACGGCGGCTGATTATGAGTTTTCAAGCTCTGCCTCCTGGGTCGATGTTGATGCTa                         >  1:694070/1‑140 (MQ=255)                                                                                                             gACCTACCGACAACTACATTCCCTTAGCAGGGGTTCACCGGGGCGTATTATCAGTTGAATAATGACAACTTTGCCCCAGGAAAAACGGCGGCTGATTATGAGTTTTCAAGCTCTGCCTCCTGGGTCGATGTTGATGCTAc                        <  1:783095/140‑1 (MQ=255)                                                                                                              aCCTACCAACAACTACAGTCCCTTAGTAGGGGTTCACCGGGGCGTGTTATGAGTTGAGTAATTACAAGTTTTCCGCAGGTGAAGCGGCGGCTGATTATGGGTTTTCAAGTTTTGCCTCCGGTGTCGTTGTTGATGCTAcc                       >  2:1033322/1‑140 (MQ=255)                                                                                                                cTACCGACAACTACATTCCCTTAGCAGGGGTTCACCGGGGCGTATTATCAGTTGAATAATGACAACTTTGCCCCAGGAAAAACGGCGGCTGATTATGAGTTTTCAAGCTCTGCCTCCTGGGTCGATGTTGATGCTACCgg                     <  2:591602/140‑1 (MQ=255)                                                                                                                   ccGACAACTACATTCCCTTAGCAGGGGTTCACCGGGGCGTATTATCAGTTGAATAATGACAACTTTGCCCCAGGAAAAACGGCGGCTGATTATGAGTTTTCAAGCTCTGCCTCCTGGGTCGATGTTGATGCTACCGGTaa                  >  1:894926/1‑140 (MQ=255)                                                                                                                   ccGACAACTACATTCCCTTAGCAGGGGTTCACCGGGGCGTATTATCAGTTGAATAATGACAACTTTGCCCCAGGAAAAACGGCGGCTGATTATGAGTTTTCAAGCTCTGCCTCCTGGGTCGATGTTGATGCTACCGGTaa                  >  1:1115723/1‑140 (MQ=255)                                                                                                                       cAACTACATTCCCTTAGCAGGGGTTCACCGGGGCGTATTATCAGTTGAATAATGACAACTTTGCCCCAGGAAAAACGGCGGCTGATTATGAGTTTTCAAGCTCTGCCTCCTGGGTCGATGTTGATGCTACCGGTAAAGTg              <  1:804291/140‑1 (MQ=255)                                                                                                                        aaCTACATTCCCTTAGCAGGGGTTCACCGGGGCGTATTATCAGTTGAATAATGACAACTTTGCCCCAGGAAAAACGGCGGCTGATTATGAGTTTTCAAGCTCTGCCTCCTGGGTCGATGTTGATGCTACCGGTAAAGTGa             >  1:966497/1‑140 (MQ=255)                                                                                                                         aCTACATTCCCTTAGCAGGGGTTCACCGGGGCGTATTATCAGTTGAATAATGACAACTTTGCCCCAGGAAAAACGGCGGCTGATTATGAGTTTTCAAGCTCTGCCTCCTGGGTCGATGTTGATGCTACCGGTAAAGTGAc            <  2:533307/140‑1 (MQ=255)                                                                                                                           tACATTCCCTTAGCAGGGGTTCACCGGGGCGTATTATCAGTTGAATAATGACAACTTTGCCCCAGGAAAAACGGCGGCTGATTATGAGTTTTCAAGCTCTGCCTCCTGGGTCGATGTTGATGCTACCGGTAAAGTGACAt          <  2:523295/140‑1 (MQ=255)                                                                                                                                   cTTAGCAGGGGTTCACCGGGGCGTATTATCAGTTGAATAATGACAACTTTGCCCCAGGAAAAACGGCGGCTGATTATGAGTTTTCAAGCTCTGCCTCCTGGGTCGATGTTGATGCTACCGGTAAAGTGACATTTAAAAAt  <  2:694070/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                                         CTGATCCCTGTATTGAATGGTGTTCATCAAGCTGGTCTGAGTACCACAATACAATTCACTCGCGCAGAAGACAAAATAATGAGCGGTACAGTATCAGTCAATGGTACTGACCTACCGACAACTACATTCCCTTCGCAGGGGTTCACCGGGGCGTATTATCAGTTGAATAATGACAACTTTGCCCCAGGAAAAACGGCGGCTGATTATGAGTTTTCAAGCTCTGCCTCCTGGGTCGATGTTGATGCTACCGGTAAAGTGACATTTAAAAAT  >  NC_000913/2051355‑2051624

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑