Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,659,493	G→T	intergenic (‑26/+70)	hscB ← / ← iscA	co‑chaperone for [Fe‑S] cluster biosynthesis/iron‑sulfur cluster insertion protein IscA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,659,493	0	G	T	100.0%	183.5 / NA	52	intergenic (‑26/+70)	hscB/iscA	co‑chaperone for [Fe‑S] cluster biosynthesis/iron‑sulfur cluster insertion protein IscA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (28/24);  total (28/24)

TCAGGATGATACTGACGTTGTAGATCCTGAAAACGCAGGCTCAGCGCCTGGGTATCGAGTTGATAGCGGGCAGGCAAGCCAAAGAGGGTGAAGTAATCCATAACATTCTCAGGGCTGCGATCCGCCGCGATAACGGCGAATCAGGTAAAACAAACCCCACGCGCAGGCGACCACGGTGGGGTTATCGGTATGCGCATCAAACGTGGAAGCTTTCGCCGCAACCACACTCATCTTTGACGTTCGGGTTGGTGAATTTAAACCCT  >  NC_000913/2659367‑2659629                                                                                                                               |                                                                                                                                         tCAGGATGATACTGACGTTGTAGATCCTGAAAACGCAGGCTCAGCGCCTGGGTATCGAGTTGATAGCGGGCAGGCAAGCCAAAGAGGGTGAAGTAATCCATAACATTCTCAGGGCTGCGATCCGCCTCGATAACGGCGaa                                                                                                                             <  2:366613/140‑1 (MQ=255) tCAGGATGATACTGACGTTGTAGATCCTGAAAACGCAGGCTCAGCGCCTGGGTATCGAGTTGATAGCGGGCAGGCAAGCCAAAGAGGGTGAAGTAATCCATAACATTCTCAGGGCTGCGATCCGCCTCGATAACGGCGaa                                                                                                                             <  1:926858/140‑1 (MQ=255)  cAGGATGATACTGACGTTGTAGATCCTGAAAACGCAGGCTCAGCGCCTGGGTATCGAGTTGATAGCGGGCAGGCAAGCCAAAGAGGGTGAAGTAATCCATAACATTCTCAGGGCTGCGATCCGCCTCGATAACGGCGAAt                                                                                                                            <  2:62695/140‑1 (MQ=255)      atgatACTGACGTTGTAGATCCTGAAAACGCAGGCTCAGCGCCTGGGTATCGAGTTGATAGCGGGCAGGCAAGCCAAAGAGGGTGAAGTAATCCATAACATTCTCAGGGCTGCGATCCGCCTCGATAACGGCGAATCAgg                                                                                                                        <  2:521092/140‑1 (MQ=255)        gatACTGACGTTGTAGATCCTGAAAACGCAGGCTCAGCGCCTGGGTATCGAGTTGATAGCCGGCAGGCAAGCCAAAGAGGGTGAAGTAATCCATAACATTCTCAGGGCTGCGATCCGCCTCGATAACGGCGAATCAGGTa                                                                                                                      >  2:153794/1‑140 (MQ=255)          tACTGACGTTGTAGATCCTGAAAACGCAGGCTCAGCGCCTGGGTATCGAGTTGATAGCGGGCAGGCAAGCCAAAGAGGGTGAAGTAATCCATAACATTCTCAGGGCTGCGATCCGCCTCGATAACGGCGAATCAGGTaaa                                                                                                                    >  1:1101670/1‑140 (MQ=255)               aCGTTGTAGATCCTGAAAACGCAGGCTCAGCGCCTGGGTATCGAGTTGATAGCGGGCAGGCAAGCCAAAGAGGGTGAAGTAATCCATAACATTCTCAGGGCTGCGATCCGCCTCGATAACGGCGaa                                                                                                                             >  1:782394/1‑126 (MQ=255)               aCGTTGTAGATCCTGAAAACGCAGGCTCAGCGCCTGGGTATCGAGTTGATAGCGGGCAGGCAAGCCAAAGAGGGTGAAGTAATCCATAACATTCTCAGGGCTGCGATCCGCCTCGATAACGGCGaa                                                                                                                             <  2:782394/126‑1 (MQ=255)               aCGTTGTAGATCCTGAAAACGCAGGCTCAGCGCCTGGGTATCGAGTTGATAGCGGGCAGGCAAGCCAAAGAGGGTGAAGTAATCCATAACATTCTCAGGGCTGCGATCCGCCTCGATAACGGCGAATCAGGTAaaacaaa                                                                                                               >  2:574875/1‑140 (MQ=255)               aCGTTGTAGATCCTGAAAACGCAGGCTCAGCGCCTGGGTATCGAGTTGATAGCGGGCAGGCAAGCCAAAGAGGGTGAAGTAATCCATAACATTCTCAGGGCTGCGATCCGCCTCGATAACGGCGAATCAGGTAaaacaaa                                                                                                               >  2:182636/1‑140 (MQ=255)                   tGTAGATCCTGAAAACGCAGGCTCAGCGCCTGGGTATCGAGTTGATAGCGGGCAGGCAAGCCAAAGAGGGTGAAGTAATCCATAACATTCTCAGGGCTGCGATCCGCCTCGATAACGGCGAATCAGGTAAAACAAAcccc                                                                                                           >  1:431949/1‑140 (MQ=255)                    gTAGATCCTGAAAACGCAGGCTCAGCGCCTGGGTATCGAGTTGATAGCGGGCAGGCAAGCCAAAGAGGGTGAAGTAATCCATAACATTCTCAGGGCTGCGATCCGCCTCGATAACGGCGAATCAGGTAAAACAAACCCCa                                                                                                          >  1:18153/1‑140 (MQ=255)                     tAGATCCTGAAAACGCAGGCTCAGCGCCTGGGTATCGAGTTGATAGCGGGCAGGCAAGCCAAAGAGGGTGAAGTAATCCATAACATTCTCAGGGCTGCGATCCGCCTCGATAACGGCGAATCAGGTAAAACAAACCCCAc                                                                                                         >  1:129172/1‑140 (MQ=255)                        aTCCTGAAAACGCAGGCTCAGCGCCTGGGTATCGAGTTGATAGCGGGCAGGCAAGCCAAAGAGGGTGAAGTAATCCATAACATTCTCAGGGCTGCGATCCGCCTCGATAACGGCGAATCAGGTAAAACAAACCCCAcgcg                                                                                                      <  2:431949/140‑1 (MQ=255)                          ccTGAAAACGCAGGCTCAGCGCCTGGGTATCGAGTTGATAGCGGGCAGGCAAGCCAAAGAGGGTGAAGTAATCCATAACATTCTCAGGGCTGCGATCCGCCTCGATAACGGCGAATCAGGTAAAACAAACCCCACGCGCa                                                                                                    <  1:303229/140‑1 (MQ=255)                          ccTGAAAACGCAGGCTCAGCGCCTGGGTATCGAGTTGATAGCGGGCAGGCAAGCCAAAGAGGGTGAAGTAATCCATAACATTCTCAGGGCTGCGATCCGCCTCGATAACGGCGAATCAGGTAAAACAAACCCCACGCGCa                                                                                                    >  1:7999/1‑140 (MQ=255)                            tGAAAACGCACGCTCAGCGCCTGGGAATCGAGGTGATAGCGGGCAGACAAGCCAAAGAGGGTGCAGTAATCCATAACATTCTCAGGGCTGCGATCCGCCTCGATAACGGCGAATCAGGGAAAACAAACCCCACGCGCAgg                                                                                                  >  2:941193/1‑140 (MQ=255)                               aaaCGCAGGCTCAGCGCCTGGGTATCGAGTTGATAGCCGGCAGGCAAGCCAAAGAGGGTGAAGTAATCCATAACATTCTCAGGGCTGCGATCCGCCTCGATAACGGCGAATCAGGTAAAACAAACCCCACGCGCAGGCGa                                                                                               <  1:153794/140‑1 (MQ=255)                                  cGCAGGCTCAGCGCCTGGGTATCGAGTTGATAGCGGGCAGGCAAGCCAAAGAGGGTGAAGTAATCCATAACATTCTCAGGGCTGCGATCCGCCTCGATAACGGCGAATCAGGTAAAACAAACCCCACGCGCAGGCGACCa                                                                                            >  2:388634/1‑140 (MQ=255)                                      ggCTCAGCGCCTGGGTATCGAGTTGATAGCGGGCAGGCAAGCCAAAGAGGGTGAAGTAATCCATAACATTCTCAGGGCTGCGATCCGCCTCGATAACGGCGAATCAGGTAAAACAAACCCCACGCGCAGGCGACCACGGt                                                                                        <  2:716417/140‑1 (MQ=255)                                      ggCTCAGCGCCTGGGTATCGAGTTGATAGCGGGCAGGCAAGCCAAAGAGGGTGAAGTAATCCATAACATTCTCAGGGCTGCGATCCGCCTCGATAACGGCGAATCAGGTAAAACAAACCCCACGCGCAGGCGACCACGGt                                                                                        <  2:18153/140‑1 (MQ=255)                                      ggCTCAGCGCCTGGGTATCGAGTTGATAGCGGGCAGGCAAGCCAAAGAGGGTGAAGTAATCCATAACATTCTCAGGGCTGCGATCCGCCTCGATAACGGCGAATCAGGTAAAACAAACCCCACGCGCAGGCGACCACGGt                                                                                        <  1:388634/140‑1 (MQ=255)                                          cAGCGCCTGGGTATCGAGTTGATAGCGGGCAGGCAAGCCAAAGAGGGTGAAGTAATCCATAACATTCTCAGGGCTGCGATCCGCCTCGATAACGGCGAATCAGGTAAAACAAACCCCACGCGCAGGCGACCACGGTgggg                                                                                    <  2:1101670/140‑1 (MQ=255)                                            gcgcCTGGGTATCGAGTTGATAGCGGGCAGGCAAGCCAAAGAGGGTGAAGTAATCCATAACATTCTCAGGGCTGCGATCCGCCTCGATAACGGCGAATCAGGTAAAACAAACCCCACGCGCAGGCGACCACGGTGGGGtt                                                                                  <  2:1030715/140‑1 (MQ=255)                                             cgcCTGGGTATCGAGTTGATAGCGGGCAGGCAAGCCAAAGAGGGTGAAGTAATCCATAACATTCTCAGGGCTGCGATCCGCCTCGATAACGGCGAATCAGGTAAAACAAACCCCACGCGCAGGCGACCACGGTGGGGTTa                                                                                 >  2:1058313/1‑140 (MQ=255)                                                       tCGAGTTGATAGCGGGCAGGCAAGCCAAAGAGGGTGAAGTAATCCATAACATTCTCAGGGCTGCGATCCGCCTCGATAACGGCGAATCAGGTAAAACAAACCCCACGCGCAGGCGACCACGGTGGGGTTATCGGTATgcg                                                                       >  1:322413/1‑140 (MQ=255)                                                                     ggcaggcaAGCCAAAGAGGGTGAAGTAATCCATAACATTCTCAGGGCTGCGATCCGCCTCGATAACGGCGAATCAGGTAAAACAAACCCCACGCGCAGGCGACCACGGTGGGGTTATCGGTATGCGCATCAAACGTGGaa                                                         >  1:14216/1‑140 (MQ=255)                                                                               ccAAAGAGGGTGAAGTAATCCATAACATTCTCAGGGCTGCGATCCGCCTCGATAACGGCGAATCAGGTAAAACAAACCCCACGCGCAGGCGACCACGGTGGGGTTATCGGTATGCGCATCAAACGTGGAAGCTTTcgccg                                               <  1:1058313/140‑1 (MQ=255)                                                                                   agagGGTGAAGTAATCCATAACATTCTCAGGGCTGCGATCCGCCTCGATAACGGCGAATCAGGTAAAACAAACCCCACGCGCAGGCGACCACGGTGGGGTTATCGGTATGCGCATCAAACGTGGAAGCTTTCGCCGCAAc                                           <  2:7999/140‑1 (MQ=255)                                                                                         tGAAGTAATCCATAACATTCTCAGGGCTGCGATCCGCCTCGATAACGGCGAATCAGGTAAAACAAACCCCACGCGCAGGCGACCACGGTGGGGTTATCGGTATGCGCATCAAACGTGGAAGCTTTcgccg                                               <  1:669975/130‑1 (MQ=255)                                                                                         tGAAGTAATCCATAACATTCTCAGGGCTGCGATCCGCCTCGATAACGGCGAATCAGGTAAAACAAACCCCACGCGCAGGCGACCACGGTGGGGTTATCGGTATGCGCATCAAACGTGGAAGCTTTcgccg                                               >  2:669975/1‑130 (MQ=255)                                                                                         tGAAGTAATCCATAACATTCTCAGGGCTGCGATCCGCCTCGATAACGGCGAATCAGGTAAAACAAACCCCACGCGCAGGCGACCACGGAGGGGTTATCGGTATGCGCATCAAACGTGGAAGCTTTCGCCGCAACCACACt                                     >  2:622428/1‑140 (MQ=255)                                                                                                 tCCATAACATTCTCAGGGCTGCGATCCGCCTCGATAACGGCGAATCAGGTAAAACAAACCCCACGCGCAGGCGACCACGGTGGGGTTATCGGTATGCGCATCAAACGTGGAAGCTTTCGCCGCAACCACACTCATCTTTg                             >  1:691034/1‑140 (MQ=255)                                                                                                     tAACATTCTCAGGGCTGCGATCCGCCTCGATAACGGCGAATCAGGTAAAACAAACCCCACGCGCAGGCGACCACGGTGGGGTTATCGGTATGCGCATCAAACGTGGAAGCTTTCGCCGCAACCACACTCATCTTTGACGt                         >  1:364574/1‑140 (MQ=255)                                                                                                      aaCATTCTCAGGGCTGCGATCCGCCTCGATAACGGCGAATCAGGTAAAACAAACCCCACGCGCAGGCGACCACGGTGGGGTTATCGGTATGCGCATCAAACGTGGAAGCTTTCGCCGCAACCACACTCATCTTTGACGtt                        >  1:400982/1‑140 (MQ=255)                                                                                                      aaCATTCTCAGGGCTGCGATCCGCCTCGATAACGGCGAATCAGGTAAAACAAACCCCACGCGCAGGCGACCACGGTGGGGTTATCGGTATGCGCATCAAACGTGGAAGCTTTCGCCGCAACCACACTCATCTTTGACGtt                        <  1:805317/140‑1 (MQ=255)                                                                                                           tctcAGGGCTGCGATCCGCCTCGATAACGGCGAATCAGGTAAAACAAACCCCACGCGCAGGCGACCACGGTGGGGTTATCGGTATGCGCATCAAACGTGGAAGCTTTCGCCGCAACCACACTCATCTTTGACGTTCGGGt                   >  1:1023767/1‑140 (MQ=255)                                                                                                           tctcAGGGCTGCGATCCGCCTCGATAACGGCGAATCAGGTAAAACAAACCCCACGCGCAGGCGACCACGGTGGGGTTATCGGTATGCGCATCAAACGTGGAAGCTTTCGCCGCAACCACACTCATCTTTGACGTTCGGGt                   >  1:804363/1‑140 (MQ=255)                                                                                                             tcAGGGCTGCGATCCGCCTCGATAACGGCGAATCAGGTAAAACAAACCCCACGCGCAGGCGACCACGGTGGGGTTATCGGTATGCGCATCAAACGTGGAAGCTTTCGCCGCAACCACACTCATCTTTGACGTTCGGGTTg                 >  1:552606/1‑140 (MQ=255)                                                                                                               aGGGCTGCGATCCGCCTCGATAACGGCGAATCAGGTAAAACAAACCCCACGCGCAGGCGACCACGGTGGGGTTATCGGTATGCGCATCAAACGTGGAAGCTTTCGCCGCAACCACACTCATCTTTGACGTTCGGGTTGGt               >  2:190312/1‑140 (MQ=255)                                                                                                               aGGGCTGCGATCCGCCTCGATAACGGCGAATCAGGTAAAACAAACCCCACGCGCAGGCGACCACGGTGGGGTTATCGGTATGCGCATCAAACGTGGAAGCTTTCGCCGCAACCACACTCATCTTTGACGTTCGGGTTGGt               <  2:322413/140‑1 (MQ=255)                                                                                                                gggCTGCGATCCGCCTCGATAACGGCGAATCAGGTAAAACAAACCCCACGCGCAGGCGACCACGGTGGGGTTATCGGTATGCGCATCAAACGTGGAAGCTTTCGCCGCAACCACACTCATCTTTGACGtt                        <  2:954056/130‑1 (MQ=255)                                                                                                                gggCTGCGATCCGCCTCGATAACGGCGAATCAGGTAAAACAAACCCCACGCGCAGGCGACCACGGTGGGGTTATCGGTATGCGCATCAAACGTGGAAGCTTTCGCCGCAACCACACTCATCTTTGACGtt                        >  1:954056/1‑130 (MQ=255)                                                                                                                gggCTGCGATCCGCCTCGATAACGGCGAATCAGGTAAAACAAACCCCACGCGCAGGCGACCACGGTGGGGTTATCGGTATGCGCATCAAACGTGGAAGCTTTCGCCGCAACCACACTCATCTTTGACGTTCGGGTTGGTg              <  1:182636/140‑1 (MQ=255)                                                                                                                 ggCTGCGATCCGCCTCGATAACGGCGAATCAGGTAAAACAAACCCCACGCGCAGGCGACCACGGTGGGGTTATCGGTATGCGCATCAAACGTGGAAGCTTTCGCCGCAACCACACTCATCtt                               >  2:1056246/1‑122 (MQ=255)                                                                                                                 ggCTGCGATCCGCCTCGATAACGGCGAATCAGGTAAAACAAACCCCACGCGCAGGCGACCACGGTGGGGTTATCGGTATGCGCATCAAACGTGGAAGCTTTCGCCGCAACCACACTCATCtt                               <  1:1056246/122‑1 (MQ=255)                                                                                                                 ggCTGCGATCCGCCTCGATAACGGCGAATCAGGTAAAACAAACCCCACGCGCAGGCGACCACGGTGGGGTTATCAGTATGCGCATCAAACGTGGAAGCTTTCGCCGCAACCACACTCATCTTTGACGTTCggg                    <  2:587386/133‑1 (MQ=255)                                                                                                                 ggCTGCGATCCGCCTCGATAACGGCGAATCAGGTAAAACAAACCCCACGCGCAGGCGACCACGGTGGGGTTATCAGTATGCGCATCAAACGTGGAAGCTTTCGCCGCAACCACACTCATCTTTGACGTTCggg                    >  1:587386/1‑133 (MQ=255)                                                                                                                  gCTGCGATCCGCCTCGATAACGGCGAATCAGGTAAAACAAACCCCACGCGCAGGCGACCACGGTGGGGTTATCGGTATGCGCATCAAACGTGGAAGCTTTCGCCGCAACCACACTCATCTTTGACGTTCggg                    >  1:839385/1‑132 (MQ=255)                                                                                                                  gCTGCGATCCGCCTCGATAACGGCGAATCAGGTAAAACAAACCCCACGCGCAGGCGACCACGGTGGGGTTATCGGTATGCGCATCAAACGTGGAAGCTTTCGCCGCAACCACACTCATCTTTGACGTTCggg                    <  2:839385/132‑1 (MQ=255)                                                                                                                  gCTGCGATCCGCCTCGATAACGGCGAATCAGGTAAAACAAACCCCACGCGCAGGCGACCACGGTGGGGTTATCGGTATGCGCATCAAACGTGGAAGCTTTCGCCGCAACCACACTCATCTTTGACGTTCGGGTTGGTGaa            >  2:108944/1‑140 (MQ=255)                                                                                                                   cTGCGATCCGCCTCGATAACGGCGAATCAGGTAAAACAAACCCCACGCGCAGGCGACCACGGTGGGGTTATCGGTATGCGCATCAAACGTGGAAGCTTTCGCCGCAACCACACTCATCTTTGACGTTCGGGTTGGTGAAt           >  1:827332/1‑140 (MQ=255)                                                                                                                       gATCCGCCTCGATAACGGCGAATCAGGTAAAACAAACCCCACGCGCAGGCGACCACGGTGGGGTTATCGGTATGCGCATCAAACGTGGAAGCTTTCGCCGCAACCACACTCATCTTTGACGTTCGGGTTGGTGAATTTaa       <  2:1158908/140‑1 (MQ=255)                                                                                                                           cgcctcGATAACGGCGAATCAGGTAAAACAAACCCCACGCGCAGGCGACCACGGTGGGGTTATCGGTATGCGCATCAAACGTGGAAGCTTTCGCCGCAACCACACTCATCTTTGACGTTCGGGTTGGTGAATTTAAAccc   <  2:387198/140‑1 (MQ=255)                                                                                                                           cgcctcGATAACGGCGAATCAGGTAAAACAAACCCCACGCGCAGGCGACCACGGTGGGGTTATCGGTATGCGCATCAAACGTGGAAGCTTTCGCCGCAACCACACTCATCTTTGACGTTCGGGTTGGTGAATTTAAAccc   >  2:1132216/1‑140 (MQ=255)                                                                                                                           cgcctcGATAACGGCGAATCAGGTAAAACAAACCCCACGCGCAGGCGACCACGGTGGGGTTATCGGTATGCGCATCAAACGTGGAAGCTTTCGCCGCAACCACACTCATCTTTGACGTTCGGGTTGGTGAATTTAAAccc   >  1:387198/1‑140 (MQ=255)                                                                                                                            gcctcGATAACGGCGAATCAGGTAAAACAAACCCCACGCGCAGGCGACCACGGTGGGGTTATCGGTATGCGCATCAAACGTGGAAGCTTTCGCCGCAACCACACTCATCTTTGACGTTCGGGTTGGTGAATTTAAACCCt  <  1:108944/140‑1 (MQ=255)                                                                                                                               |                                                                                                                                         TCAGGATGATACTGACGTTGTAGATCCTGAAAACGCAGGCTCAGCGCCTGGGTATCGAGTTGATAGCGGGCAGGCAAGCCAAAGAGGGTGAAGTAATCCATAACATTCTCAGGGCTGCGATCCGCCGCGATAACGGCGAATCAGGTAAAACAAACCCCACGCGCAGGCGACCACGGTGGGGTTATCGGTATGCGCATCAAACGTGGAAGCTTTCGCCGCAACCACACTCATCTTTGACGTTCGGGTTGGTGAATTTAAACCCT  >  NC_000913/2659367‑2659629

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑