Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,565,138	G→T	P238Q (CCG→CAG)	yjiM ←	putative dehydratase subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,565,138	0	G	T	100.0%	232.1 / NA	67	P238Q (CCG→CAG)	yjiM	putative dehydratase subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (28/39);  total (28/39)

GCTCGGTCGCTTTCGCCCCGGTGCAGTTTTCATAACCGACAACCCAGCCGCCATTCTCTTCAATCGCGCGCACCACTTTTTCTGCTGCGCCGCCAATCGGGCAGCCGGTGATTAAAATGCGCGGACGCGGGTCCAGTCGCTGGCCTTCTTCCCACTGCTGACGAACGCGGGCGGTCATTGCATCCAGTTCATTGATCAACGCCTCTTTATCGAACCGGAAGGTTGCGCCGTAAACCACTTTCAGAATGTCGCTGCCGCTA  >  NC_000913/4565010‑4565269                                                                                                                                 |                                                                                                                                    gCTCGGTCGCTTTCGCCCCGGTGCAGTTTTCATAACCGACAACCCAGCCGCCATTCTCTTCAATCGCGCGCACCACTTTTTCTGCTGCGCCGCCAATCGGGCAGCCGGTGATTAAAATGCGCGGACGCTGGTCCAGTCGc                                                                                                                          <  2:245541/140‑1 (MQ=255)  cTCGGTCGCTTTCGCCCCGGTGCAGTTTTCATAACCGACAACCCAGCCGCCATTCTCTTCAATCGCGCGCACCACTTTTTCTGCTGCGCCGCCAATCGGGCAGCCGGTGATTAAAATGCGCGGACGCTGGTCCAGTCGCt                                                                                                                         >  1:957868/1‑140 (MQ=255)   tCGGTCGCTTTCGCCCCGGTGCAGTTTTCATAACCGACAACCCAGCCGCCATTCTCTTCAATCGCGCGCACCACTTTTTCTGCTGCGCCGCCAATCGGGCAGCCGGTGATTAAAATGCGCGGACGCTGGTCCAGTCGCTg                                                                                                                        <  2:1006692/140‑1 (MQ=255)   tCGGTCGCTTTCGCCCCGGTGCAGTTTTCATAACCGACAACCCAGCCGCCATTCTCCTCAATCGCGCGCACCACTTTTTCTGCTGCGCCGCCAATCGGGCAGCCGGTGATTAAAATGCGCGGACGCTGGTCCAGTCGCTg                                                                                                                        <  1:798012/140‑1 (MQ=255)     ggTCGCTTTCGCCCCGGTGCAGTTTTCATAACCGACAACCCAGCCGCCATTCTCTTCAATCGCGCGCACCACTTTTTCTGCTGCGCCGCCAATCGGGCAGCCGGTGATTAAAATGCGCGGACGCTGGTCCAGTCGCTGGc                                                                                                                      <  1:1061318/140‑1 (MQ=255)       tCGCTTTCGCCCCGGTGCAGTTTTCATAACCGACAACCCAGCCGCCATTCTCTTCAATCGCGCGCACCACTTTTTCTGCTGCGCCGCCAATCGGGCAGCCGGTGATTAAAATGCGCGGACGCTGGTCCAGTCGCTGGCct                                                                                                                    >  2:272443/1‑140 (MQ=255)       tCGCTTTCGCCACGGTGCAGTTTTCATAACCGACAACCCAGCCGCCATTCTCTTCAATCGCGCGCACCACTTTTTCTGCTGCGCCGCCAATCGGGCAGCCGGTGATTAAAATGCGCGGACGCTGGTCCAGTCGCTGGCct                                                                                                                    >  2:989203/1‑140 (MQ=255)        cGCTTTCGCCCCGGTGCAGTTTTCATAACCGACAACCCAGCCGCCATTCTCTTCAATCGCGCGCACCACTTTTTCTGCTGCGCCGCCAATCGGGCAGCCGGTGATTAAAATGCGCGGACGCTGGTCCAGTCGCTGGCctt                                                                                                                   >  2:164698/1‑140 (MQ=255)          cTTTCGCCCCGGTGCAGTTTTCATAACCGACAACCCAGCCGCCATTCTCTTCAATCGCGCGCACCACTTTTTCTGCTGCGCCGCCAATCGGGCAGCCGGTGTTTAAAATGCGCGGACGCTGGTCCAGCCGCTGCCctttt                                                                                                                 >  2:115209/1‑138 (MQ=255)          cTTTCGCCCCGGTGCAGTTTTCATAACCGACAACCCAGCCGCCATTCTCTTCAATCGCGCGCACCACTTTTTCTGCTGCGCCGCCAATCGGGCAGCCGGTGATTAAAATGCGCGGACGCTGGTCCAGTCGCTGGCcttct                                                                                                                 <  1:590380/140‑1 (MQ=255)          cTTTCGCCCCGGTGCAGTTTTCATAACCGACAACCCAGCCGCCATTCTCTTCAATCGCGCGCACCACTTTTTCTGCTGCGCCGCCAATCGGGCAGCCGGTGATTAAAATGCGCGGACGCTGGTCCAGTCGCTGGCcttct                                                                                                                 >  2:1097379/1‑140 (MQ=255)               gCCCCGGTGCAGTTTTCATAACCGACAACCCAGCCGCCATTCTCTTCAATCGCGCGCACCACTTTTTCTGCTGCGCCGCCAATCGGGCAGCCGGTGATTAAAATGCGCGGACGCTGGTCCAGTCGCTGGCCTTCTTCCCa                                                                                                            <  1:272443/140‑1 (MQ=255)                     gTGCAGTTTTCATAACCGACAACCCAGCCGCCATTCTCTTCAATCGCGCGCACCACTTTTTCTGCTGCGCCGCCAATCGGGCAGCCGGTGATTAAAATGCGCGGACGCTGGTCCAGTCGCTGGCCTTCTTCCCActgctg                                                                                                      >  1:440570/1‑140 (MQ=255)                        cAGTTTTCATAACCGACAACCCAGCCGCCATTCTCTTCAATCGCGCGCACCACTTTTTCTGCTGCGCCGCCAATCGGGCAGCCGGTGATTAAAATGCGCGGACGCTGGTCCAGTCGCTGGCCTTCTTCCCACTGCTGACg                                                                                                   <  1:143960/140‑1 (MQ=255)                             ttCATAACCGACAACCCAGCCGCCATTCTCTTCAATCGCGCGCACCACTTTTTCTGCTGCGCCGCCAATCGGGCAGCCGGTGATTAAAATGCGCGGACGCTGGTCCAGTCGCTGGCCTTCTTCCCACTGCTGACGAAcgc                                                                                              >  2:454685/1‑140 (MQ=255)                              tCATAACCGCCAACCCAGCCGCCATTCTCTTCAATCGCGCGCACCACTTTTTCTGCTGCGCCGCCAATCGGGCAGCCGGTGATTAAAATGCGCGGACGCTGGTCCAGTCGCTGGCCTTCTTCCCACTGCTGACGAAcgcg                                                                                             <  1:115209/140‑1 (MQ=255)                              tCATAACCGACAACCCAGCCGCCATTCTCTTCAATCGCGCGCACCACTTTTTCTGCTGCGCCGCCAATCGGGCAGCCGGTGATTAAAATGCGCGGACGCTGGTCCAGTCGCTGGCCTTCTTCCCACTGCTGACGAAcgcg                                                                                             <  1:470905/140‑1 (MQ=255)                              tCATAACCGACAACCCAGCCGCCATTCTCTTCAATCGCGCGCACCACTTTTTCTGCTGCGCCGCCAATCGGGCAGCCGGTGATTAAAATGCGCGGACGCTGGTCCAGTCGCTGGCCTTCTTCCCACTGCTGACGAAcgcg                                                                                             <  2:957868/140‑1 (MQ=255)                                aTAACCGACAACCCAGCCGCCATTCTCTTCAATCGCGCGCACCACTTTTTCTGCTGCGCCGCCAATCGGGCAGCCGGTGATTAAAATGCGCGGACGCTGGTCCAGTCGCTGGCCTTCTTCCCACTGCTGACGAACgcggg                                                                                           <  2:1109516/140‑1 (MQ=255)                                   aCCGACAACCCAGCCGCCATTCTCTTCAATCGCGCGCACCACTTTTTCTGCTGCGCCGCCAATCGGGCAGCCGGTGATTAAAATGCGCGGACGCTGGTCCAGTCGCTGGCCTTCTTCCCACTGCTGACGAACgcgggcgg                                                                                        <  1:800185/140‑1 (MQ=255)                                    ccGACAACCCAGCCGCCATTCTCTTCAATCGCGCGCACCACTTTTTCTGCTGCGCCGCCAATCGGGCAGCCGGTGATTAAAATGCGCGGACGCTGGTCCAGTCGCTGGCCTTCTTCCCACTGCTGACGAACGCGGGCGGt                                                                                       >  1:506936/1‑140 (MQ=255)                                       aCAACCCAGCCGCCATTCTCTTCAATCGCGCGCACCACTTTTTCTGCTGCGCCGCCAATCGGGCAGCCGGTGATTAAAATGCGCGGACGCTGGTCCAGTCGCTGGCCTTCTTCCCACTGCTGACGAACGCGGGCGGTCAt                                                                                    >  1:82492/1‑140 (MQ=255)                                       aCAACCCAGCCGCCATTCTCTTCAATCGCGCGCACCACTTTTTCTGCTGCGCCGCCAATCGGGCAGCCGGTGATTAAAATGCGCGGACGCTGGTCCAGTCGCTGGCCTTCTTCCCACTGCTGACGAACGCGGGCGGTCAt                                                                                    >  2:1134815/1‑140 (MQ=255)                                          aCCCAGCCGCCATTCTCTTCAATCGCGCGCACCACTTTTTCTGCTGCGCCGCCAATCGGGCAGCCGGTGATTAAAATGCGCGGACGCTGGTCCAGTCGCTGGCCTTCTTCCCACTGCTGACGAACGCGGGCGGTCATTGc                                                                                 >  2:7064/1‑140 (MQ=255)                                           cccAGCCGCCATTCTCTTCAATCGCGCGCACCACTTTTTCTGCTGCGCCGCCAATCGGGCAGCCGGTGATTAAAATGCGCGGACGCTGGTCCAGTCGCTGGCCTTCTTCCCACTGCTGACGAACGCGGGCGGTCATTGCa                                                                                >  2:359737/1‑140 (MQ=255)                                             cAGCCGCCATTCTCTTCAATCGCGCGCACCACTTTTTCTGCTGCGCCGCCAATCGGGCAGCCGGTGATTAAAATGCGCGGACGCTGGTCCAGTCGCTGGCCTTCTTCCCACTGCTGACGAACGCGGGCGGTCATTGCATc                                                                              <  1:359737/140‑1 (MQ=255)                                             cAGCCGCCATTCTCTTCAATCGCGCGCACCACTTTTTCTGCTGCGCCGCCAATCGGGCAGCCGGTGATTAAAATGCGCGGACGCTGGTCCAGTCGCTGGCCTTCTTCCCACTGCTGACGAACGCGGGCGGTCATTGCATc                                                                              <  2:359802/140‑1 (MQ=255)                                              aGCCGCCATTCTCTTCAATCGCGCGCACCACTTTTTCTGCTGCGCCGCCAATCGGGCAGCCGGTGATTAAAATGCGCGGACGCTGGTCCAGTCGCTGGCCTTCTTCCCACTGCTGACGAACGCGGGCGGTCAtt                                                                                   <  1:629892/134‑1 (MQ=255)                                              aGCCGCCATTCTCTTCAATCGCGCGCACCACTTTTTCTGCTGCGCCGCCAATCGGGCAGCCGGTGATTAAAATGCGCGGACGCTGGTCCAGTCGCTGGCCTTCTTCCCACTGCTGACGAACGCGGGCGGTCAtt                                                                                   >  2:629892/1‑134 (MQ=255)                                              aGCCGCCATTCTCTTCAATCGCGCGCACCACTTTTTCTGCTGCGCCGCCAATCGGGCAGCCGGTGATTAAAATGCGCGGACGCTGGTCCAGTCGCTGGCCTTCTTCCCACTGCTGACGAACGCGGGCGGTCATTGCATcc                                                                             <  1:1087997/140‑1 (MQ=255)                                               gccgccATTCTCTTCAATCGCGCGCACCACTTTTTCTGCTGCGCCGCCAATCGGGCAGCCGGTGATTAAAATGCGCGGACGCTGGTCCAGTCGCTGGCCTTCTTCCCACTGCTGACGAACGCGGGCGGTCATTGCATCCa                                                                            <  1:978785/140‑1 (MQ=255)                                               gccgccATTCTCTTCAATCGCGCGCACCACTTTTTCTGCTGCGCCGCCAATCGGGCAGCCGGTGATTAAAATGCGCGGACGCTGGTCCAGTCGCTGGCCTTCTTCCCACTGCTGACGAACGCGGGCGGTCATTGCATCCa                                                                            <  2:82492/140‑1 (MQ=255)                                               gccgccATTCTCTTCAATCGCGCGCACCACTTTTTCTGCTGCGCCGCCAATCGGGCAGCCGGTGATTAAAATGCGCGGACGCTGGTCCAGTCGCTGGCCTTCTTCCCACTGCTGACGAACGCGGGCGGTCATTGCATCCa                                                                            <  2:838025/140‑1 (MQ=255)                                                    cATTCTCTTCAATCGCGCGCACCACTTTTTCTGCTGCGCCGCCAATCGGGCAGCCGGTGATTAAAATGCGCGGACGCTGGTCCAGTCGCTGGCCTTCTTCCCACTGCTGACGAACGCGGGCGGTCATTGCATCCAGTTCa                                                                       <  2:506936/140‑1 (MQ=255)                                                     aTTCTCTTCAATCGCGCGCACCACTTTTTCTGCTGCGCCGCCAATCGGGCAGCCGGTGATTAAAATGCGCGGACGCTGGTCCAGTCGCTGGCCTTCTTCCCACTGCTGACGAACGCGGGCGGTCATTGCATCCAGTTCAt                                                                      >  1:149731/1‑140 (MQ=255)                                                             cAATCGCGCGCACCACTTTTTCTGCTGCGCCGCCAATCGGGCAGCCGGTGATTAAAATGCGCGGACGCTGGTCCAGTCGCTGGCCTTCTTCCCACTGCTGACGAACGCGGGCGGTCATTGCATCCAGTTCATTGATCAAc                                                              <  1:164698/140‑1 (MQ=255)                                                              agtCGCGCGCACCGCTTTTTCTGCTGCGCCGCCAATCGGGCAGCCGGTGATTAAAATGCGCGGACGCTGGTCCAGTCGCTGGCCTTCTTCCCACTGCTGACGAACGCGGGCGGTCATTGCATCCAGTTCATTGATCAACg                                                             >  2:876955/3‑140 (MQ=255)                                                              aaTCGCGCGCACCACTTTTTCTGCTGCGCCGCCAATCGGGCAGCCGGTGATTAAAATGCGCGGACGCTGGTCCAGTCGCTGGCCTTCTTCCCACTGCTGACGAACGCGGGCGGTCATTGCATCCAGTTCATTGATCAACg                                                             <  1:233452/140‑1 (MQ=255)                                                                 cgcgcgCACCACTTTTTCTGCTGCGCCGCCAATCGGGCAGCCGGTGATTAAAATGCGCGGACGCTGGTCCAGTCGCTGGCCTTCTTCCCACTGCTGACGAACGCGGGCGGTCATTGCATCCAGTTCATTGATCAACGCct                                                          >  1:838504/1‑140 (MQ=255)                                                                   cgcgcACCACTTTTTCTGCTGCGCCGCCAATCGGGCAGCCGGTGATTAAAATGCGCGGACGCTGGTCCAGTCGCTGGCCTTCTTCCCACTGCTGACGAACGCGGGCGGTCATTGCATCCAGTTCATTGATCAACGCctct                                                        >  1:751864/1‑140 (MQ=255)                                                                   cgcgcACCACTTTTTCTGCTGCGCCGCCAATCGGGCAGCCGGTGATTAAAATGCGCGGACGCTGGTCCAGTCGCTGGCCTTCTTCCCACTGCTGACGAACGCGGGCGGTCATTGCATCCAGTTCATTGATCAACGCctct                                                        >  2:769215/1‑140 (MQ=255)                                                                     cgcACCACTTTTTCTGCTGCGCCGCCAATCGGGCAGCCGGTGATTAAAATGCGCGGACGCTGGTCCAGTCGCTGGCCTTCTTCCCACTGCTGACGAACGCGGGCGGTCATTGCATCCAGTTCATTGATCAACGCCTCttt                                                      >  1:761230/1‑140 (MQ=255)                                                                         ccacTTTTTCTGCTGCGCCGCCAATCGGGCAGCCGGTGATTAAAATGCGCGGACGCTGGTCCAGTCGCTGGCCTTCTTCCCACTGCTGACGAACGCGGGCGGTCATTGCATCCAGTTCATTGATCAACGCCTCTTTATCg                                                  <  2:345445/140‑1 (MQ=255)                                                                         ccacTTTTTCTGCTGCGCCGCCAATCGGGCAGCCGGTGATTAAAATGCGCGGACGCTGGTCCAGTCGCTGGCCTTCTTCCCACTGCTGACGAACGCGGGCGGTCATTGCATCCAGTTCATTGATCAACGCCTCTTTATCg                                                  <  2:761230/140‑1 (MQ=255)                                                                         ccacTTTTTCTGCTGCGCCGCCAATCGGGCAGCCGGTGATTAAAATGCGCGGACGCTGGTCCAGTCGCTGGCCTTCTTCCCACTGCTGACGAACGCGGGCGGTCATTGCATCCAGTTCATTGATCAACGCCTCTTTATCg                                                  <  1:454685/140‑1 (MQ=255)                                                                                ttCTGCTGCGCCGCCAATCGGGCAGCCGGTGATTAAAATGCGCGGACGCTGGTCCAGTCGCTGGCCTTCTTCCCACTGCTGACGAACGCGGGCGGTCATTGCATCCAGTTCATTGATCAACGCCTCTTTATCGAACCGGa                                           <  2:751864/140‑1 (MQ=255)                                                                                  ctgctgCGCCGCCAATCGGGCAGCCGGTGATTAAAATGCGCGGACGCTGGTCCAGTCGCTGGCCTTCTTCCCACTGCTGACGAACGCGGGCGGTCATTGCATCCAGTTCATTGATCAACGCCTCTTTATCGAACCGGAAg                                         >  2:466518/1‑140 (MQ=255)                                                                                     ctgcGCCGCCAATCGGGCAGCCGGTGATTAAAATGCGCGGACGCTGGTCCAGTCGCTGGCCTTCTTCCCACTGCTGACGAACGCGGGCGGTCATTGCATCCAGTTCATTGATCAACGCCTCTTTATCGAACCGGAAGGtt                                      <  2:724730/140‑1 (MQ=255)                                                                                      tgcGCCGCCAATCGGGCAGCCGGTGATTAAAATGCGCGGACGCTGGTCCAGTCGCTGGCCTTCTTCCCACTGCTGACGAACGCGGGCGGTCATTGCATCCAGTTCATTGATCAACGCCTCTTTATCGAACCGGAAGGTTg                                     <  1:989203/140‑1 (MQ=255)                                                                                      tgcGCCGCCAATCGGGCAGCCGGTGATTAAAATGCGCGGACGCTGGTCCAGTCGCTGGCCTTCTTCCCACTGCTGACGAACGCGGGCGGTCATTGCATCCAGTTCATTGATCAACGCCTCTTTATCGAACCGGAAGGTTg                                     <  1:466518/140‑1 (MQ=255)                                                                                      tgcGCCGCCAATCGGGCAGCCGGTGATTAAAATGCGCGGACGCTGGTCCAGTCGCTGGCCTTCTTCCCACTGCTGACGAACGCGGGCGGTCATTGCATCCAGTTCATTGATCAACGCCTCTTTATCGAACCGGAAGGTTg                                     <  1:499542/140‑1 (MQ=255)                                                                                      tgcGCCGCCAATCGGGCAGCCGGTGATTAAAATGCGCGGACGCTGGTCCAGTCGCTGGCCTTCTTCCCACTGCTGACGAACGCGGGCGGTCATTGCATCCAGTTCATTGATCAACGCCTCTTTATCGAACCGGAAGGTTg                                     >  1:511831/1‑140 (MQ=255)                                                                                       gcgcCGCCAATCGGGCAGCCGGTGATTAAAATGCGCGGACGCTGGTCCAGTCGCTGGCCTTCTTCCCACTGCTGACGAACGCGGGCGGTCATTGCATCCAGTTCATTGATCAACGCCTCTTTATCg                                                  <  2:148030/126‑1 (MQ=255)                                                                                       gcgcCGCCAATCGGGCAGCCGGTGATTAAAATGCGCGGACGCTGGTCCAGTCGCTGGCCTTCTTCCCACTGCTGACGAACGCGGGCGGTCATTGCATCCAGTTCATTGATCAACGCCTCTTTATCg                                                  >  1:148030/1‑126 (MQ=255)                                                                                         gccgccAATCGGGCAGCCGGTGATTAAAATGCGCGGACGCTGGTCCAGTCGCTGGCCTTCTTCCCACTGCTGACGAACGCGGGCGGTCATTGCATCCAGTTCATTGATCAACGCCTCTTTATCGAACCGGAAGGTTgcgc                                  <  2:149731/140‑1 (MQ=255)                                                                                               aaTCGGGCAGCCGGTGATTAAAATGCGCGGACGCTGGTCCAGTCGCTGGCCTTCTTCCCGCTGCTGACGAACGCGGGCGGTCATTGCATCCAGTTCATTGATCAACGCCTCTTTATCGAACCGGAAGGTTGCGCCGTaaa                            <  2:136059/140‑1 (MQ=255)                                                                                                aTCGGGCAGCCGGTGATTAAAATGCGCGGACGCTGGTCCAGTCGCTGGCCTTCTTCCCACTGCTGACGAACGCGGGCGGTCATTGCATCCAGTTCATTGATCAACGCCTCTTTATCGAACCGGAAGGTTGCGCCGTAAAc                           <  2:440570/140‑1 (MQ=255)                                                                                                aTCGGGCAGCCGGTGATTAAAATGCGCGGACGCTGGTCCAGTCGCTGGCCTTCTTCCCACTGCTGACGAACGCGGGCGGTCATTGCATCCAGTTCATTGATCAACGCCTCTTTATCGAACCGGAAGGTTGCGCCGTAAAc                           <  2:838504/140‑1 (MQ=255)                                                                                                      cAGCCGGTGATTAAAATGCGCGGACGCTGGTCCAGTCGCTGGCCTTCTTCCCACTGCTGACGAACGCGGGCGGTCATTGCATCCAGTTCATTGATCAACGCCTCTTTATCGAACCGGAAGGTTGCGCCGTAAACCACttt                     <  2:511831/140‑1 (MQ=255)                                                                                                        gCCGGTGATTAAAATGCGCGGACGCTGGTCCAGTCGCTGGCCTTCTTCCCACTGCTGACGAACGCGGGCGGTCATTGCATc                                                                              <  1:11172/81‑1 (MQ=255)                                                                                                        gCCGGTGATTAAAATGCGCGGACGCTGGTCCAGTCGCTGGCCTTCTTCCCACTGCTGACGAACGCGGGCGGTCATTGCATc                                                                              >  2:11172/1‑81 (MQ=255)                                                                                                         ccGGTGATTAAAATGCGCGGACGCTGGTCCAGTCGCTGGCCTTCTTCCCACTGCTGACGAACGCGGGCGGTCATTGCATCCAGTTCATTGATCAACGCCTCTTTATCGAACCGGAAGGTTgcg                                   >  2:1185331/1‑123 (MQ=255)                                                                                                         ccGGTGATTAAAATGCGCGGACGCTGGTCCAGTCGCTGGCCTTCTTCCCACTGCTGACGAACGCGGGCGGTCATTGCATCCAGTTCATTGATCAACGCCTCTTTATCGAACCGGAAGGTTgcg                                   <  1:1185331/123‑1 (MQ=255)                                                                                                         ccGGTGATTAAAATGCGCGGACGCTGGTCCAGTCGCTGGCCTTCTTCCCACTGCTGACGAACGCGGGCGGTCATTGCATCCAGTTCATTGATCAACGCCTCTTTATCGAACCGGAAGGTTGCGCCGTAAACCACTTTCAg                  >  2:420135/1‑140 (MQ=255)                                                                                                            gTGATTAAAATGCGCGGACGCTGGTCCAGTCGCTGGCCTTCTTCCCACTGCTGACGAACGCGGGCGGTCATTGCATCCAGTTCATTGATCAACGCCTCTTTATCGAACCGGAAGGTTGCGCCGTAAACCACTTTCAGAAt               >  2:538598/1‑140 (MQ=255)                                                                                                            gTGATTAAAATGCGCGGACGCTGGTCCAGTCGCTGGCCTTCTTCCCACTGCTGACGAACGCGGGCGGTCATTGCATCCAGTTCATTGATCAACGCCTCTTTATCGAACCGGAAGGTTGCGCCGTAAACCACTTTCAGAAt               >  1:787252/1‑140 (MQ=255)                                                                                                                        cgcgGACGCTGGTCCAGTCGCTGGCCTTCTTCCCACTGCTGACGAACGCGGGCGGTCATTGCATCCAGTTCATTGATCAACGCCTCTTTATCGAACCGGAAGGTTGCGCCGTAAACCACTTTCAGAATGTCGCTGCCGCt   <  2:343239/140‑1 (MQ=255)                                                                                                                         gcgGACGCTGGTCCAGTCGCTGGCCTTCTTCCCACTGCTGACGAACGCGGGCGGTCATTGCATCCAGTTCATTGATCAACGCCTCTTTATCGAACCGGAAGGTTGCGCCGTAAACCACTTTCAGAATGTCGCTGCCGCTa  >  2:662008/1‑140 (MQ=255)                                                                                                                         gcgGACGCTGGTCCAGTCGCTGGCCTTCTTCCCACTGCTGACGAACGCGGGCGGTCATTGCATCCAGTTCATTGATCAACGCCTCTTTATCGAACCGGAAGGTTGCGCCGTAAACCACTTTCAGAATGTCGCTGCCGCTa  >  2:335026/1‑140 (MQ=255)                                                                                                                                 |                                                                                                                                    GCTCGGTCGCTTTCGCCCCGGTGCAGTTTTCATAACCGACAACCCAGCCGCCATTCTCTTCAATCGCGCGCACCACTTTTTCTGCTGCGCCGCCAATCGGGCAGCCGGTGATTAAAATGCGCGGACGCGGGTCCAGTCGCTGGCCTTCTTCCCACTGCTGACGAACGCGGGCGGTCATTGCATCCAGTTCATTGATCAACGCCTCTTTATCGAACCGGAAGGTTGCGCCGTAAACCACTTTCAGAATGTCGCTGCCGCTA  >  NC_000913/4565010‑4565269

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑