Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,084,929	T→G	intergenic (+282/‑63)	efeB → / → phoH	heme‑containing peroxidase/deferrochelatase/ATP‑binding protein PhoH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,084,929	0	T	G	100.0%	133.3 / NA	37	intergenic (+282/‑63)	efeB/phoH	heme‑containing peroxidase/deferrochelatase/ATP‑binding protein PhoH
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (20/17);  total (20/17)

AATCCTGCTGAAAGCACACAGCTTTTTTCATCACTGTCATCACTCTGTCATCTTTCCAGTAGAAACTAATGTCACTGAAATGGTGTTTTATAGTTAAATATAAGTAAATATATTGTTGCAATAAATGCGAGATCTGTTGTACTTATTAAGTAGCAGCGGAAGTTCCCGGCAGTGATAGTCAGTCACTATGGAGATCGCGGATGGTAACGTCCTGTACTGGACATGTTTTAGATAATCAACGCGCCACCACTCGCGGAGTTTTCTCCTCCGG  >  NC_000913/1084792‑1085062                                                                                                                                          |                                                                                                                                      aaTCCTGCTGAAAGCACACAGCTTTTTTCATCACTGTCATCACTCTGTCATCTTTCCAGTAGAAACTAATGTCACTGAAATGGTGTTTTATAGTTAAATATAAGTAAATATATTGTTGCAATAAATGCGAGATCtgtggt                                                                                                                                     <  1:205990/140‑1 (MQ=255)       gctgAAAGCACACAGCTTTTTTCATCACTGTCATCACTCTGTCATCTTTCCAGTAGAAACTAATGTCACTGAAATGGTGTTTTATAGTTAAATATAAGTAAATATATTGTTGCAATAAATGCGAGATCTGTGGTACttat                                                                                                                               >  1:734935/1‑140 (MQ=255)                              atcaCTGTCATCACTCTGTCATCTTTCCAGTAGAAACTAATGTCACTGAAATGGTGTTTTATAGTTAAATATAAGTAAATATATTGTTGCAATAAATGCGAGATCTGTGGTACTTATTAAGTAGCAGCGGAAGTTCCTgg                                                                                                        <  1:1115272/140‑1 (MQ=255)                              atcaCTGTCATCACTCTGTCATCTTTCCAGTAGAAACTAATGTCACTGAAATGGTGTTTTATAGTTAAATATAAGTAAATATATTGTTGCAATAAATGCGAGATCTGTGGTACTTATTAAGTAGCAGCGGAAGTTCCCgg                                                                                                        <  2:369814/140‑1 (MQ=255)                              atcaCTGTCATCACTCTGTCATCTTTCCAGTAGAAACTAATGTCACTGAAATGGTGTTTTATAGTTAAATATAAGTAAATATATTGTTGCAATAAATGCGAGATCTGTGGTACTTATTAAGTAGCAGCGGAAGTTCCCgg                                                                                                        <  1:153574/140‑1 (MQ=255)                              atcaCTGTCATCACTCTGTCATCTTTCCAGTAGAAACTAATGTCACTGAAATGGTGTTTTATAGTTAAATATAAGTAAATATATTGTTGCAATAAATGCGAGATCTGTGGTACTTATTAAGTAGCAGCGGAAGTTCCCgg                                                                                                        <  1:979356/140‑1 (MQ=255)                                    gTCATCACTCTGTCATCTTTCCAGTAGAAACTAATGTCACTGAAATGGTGTTTTATAGTTAAATATAAGTAAATATATTGTTGCAATAAATGCGAGATCTGTGGTACTTATTAAGTAGCAGCGGAAGTTCCCGGCAGTGa                                                                                                  <  2:359522/140‑1 (MQ=255)                                     tcatcaCTCTGTCATCTTTCCAGTAGAAACTAATGTCACTGAAATGGTGTTTTATAGTTAAATATAAGTAAATATATTGTTGCAATAAATGCGAGATCTGTGGTACTTATTAAGTAGCAGCGGAAGTTCCCGGCAGTGAt                                                                                                 >  2:589213/1‑140 (MQ=255)                                             ctGTCATCTTTCCAGTAGAAACTAATGTCACTGAAATGGTGTTTTATAGTTAAATATAAGTAAATATATTGTTGCAATAAATGCGAGATCTGTGGTACTTATTAAGTAGCAGCGGAAGTTCCCGGCAGTGATagtcagtc                                                                                         <  2:903156/140‑1 (MQ=255)                                             ctGTCATCTTTCCAGTAGAAACTAATGTCACTGAAATGGTGTTTTATAGTTAAATATAAGTAAATATATTGTTGCAATAAATGCGAGATCTGTGGTACTTATTAAGTAGCAGCGGAAGTTCCCGGCAGTGATagtcagtc                                                                                         >  2:1184640/1‑140 (MQ=255)                                              tGTCATCTTTCCAGTAGAAACTAATGTCACTGAAATGGTGTTTTATAGTTAAATATAAGTAAATATATTGTTGCAATAAATGCGAGATCTGTGGTACTTATTAAGTAGCAGCGGAAGTTCCCGGCAGTGATAgtcagtca                                                                                        >  1:480568/1‑140 (MQ=255)                                               gTCATCTTTCCAGTAGAAACTAATGTCACTGAAATGGTGTTTTATAGTTAAATATAAGTAAATATATTGTTGCAATAAATGCGAGATCTGTGGTACTTATTAAGTAGCAGCGGAAGTTCCCGGCAGTGATagtc                                                                                             <  1:232703/134‑1 (MQ=255)                                               gTCATCTTTCCAGTAGAAACTAATGTCACTGAAATGGTGTTTTATAGTTAAATATAAGTAAATATATTGTTGCAATAAATGCGAGATCTGTGGTACTTATTAAGTAGCAGCGGAAGTTCCCGGCAGTGATagtc                                                                                             >  2:232703/1‑134 (MQ=255)                                               gTCATCTTTCCAGTAGAAACTAATGTCACTGAAATGGTGTTTTATAGTTAAATATAAGTAAATATATTGTTGCAATAAATGCGAGATCTGTGGTACTTATTAAGTAGCAGCGGAAGTTCCCGGCAGTGATAGTCAGTCAc                                                                                       >  1:14939/1‑140 (MQ=255)                                                tCATCTTTCCAGTAGAAACTAATGTCACTGAAATGGTGTTTTATAGTTAAATATAAGTAAATATATTGTTGCAATAAATGCGAGATCTGTGGTACTTATTAAGTAGCAGCGGAAGTTCCCGGCAGTGATAGTCAGTCACt                                                                                      >  1:213617/1‑140 (MQ=255)                                                tCATCTTTCCAGTAGAAACTAATGTCACTGAAATGGTGTTTTATAGTTAAATATAAGTAAATATATTGTTGCAATAAATGCGAGATCTGTGGTACTTATTAAGTAGCAGCGGAAGTTCCCGGCAGTGATAGTCAGTCACt                                                                                      >  2:350495/1‑140 (MQ=255)                                                  aTCTTTCCAGTAGAAACTAATGTCACTGAAATGGTGTTTTATAGTTAAATATAAGTAAATATATTGTTGCAATAAATGCGAGATCTGTGGTACTTATTAAGTAGCAGCGGAAGTTCCCGGCAGTGATAGTCAGTCACTAt                                                                                    <  2:213617/140‑1 (MQ=255)                                                            tAGAAACTAATGTCACTGAAATGGTGTTTTATAGTTAAATATAAGTAAATATATTGTTGCAATAAATGCGAGATCTGTGGTACTTATTAAGTAGCAGCGGAAGTTCCCGGCAGTGATAGTCAGTCACTATGGAGATcgcg                                                                          <  1:589213/140‑1 (MQ=255)                                                              gAAACTAATGTCACTGAAATGGTGTTTTATAGTTAAATATAAGTAAATATATTGTTGCAATAAATGCGAGATCTGTGGTACTTATTAAGTAGCAGCGGAAGTTCCCGGCAGTGATAGTCAGTCACTATGGAGATCGCGGa                                                                        <  2:14939/140‑1 (MQ=255)                                                               aaaCTAATGTCACTGAAATGGTGTTTTATAGTTAAATATAAGTAAATATATTGTTGCAATAAATGCGAGATCTGTGGTACTTATTAAGTAGCAGCGGAAGTTCCCGGCAGTGATAGTCAGTCACTATGGAGATCGCGGAt                                                                       >  1:768419/1‑140 (MQ=255)                                                                      tGTCACTGAAATGGTGTTTTATAGTTAAATATAAGTAAATATATTGTTGCAATAAATGCGAGATCTGTGGTACTTATTAAGTAGCAGCGGAAGTTCCCGGCAGTGATAGTCAGTCACTATGGAGATCGCGGATGGTAACg                                                                >  1:666582/1‑140 (MQ=255)                                                                        tCACTGAAATGGTGTTTTATAGTTAAATATAAGTAAATATATTGTTGCAATAAATGCGAGATCTGTGGTACTTATTAAGTAGCAGCGGAAGTTCCCGGCAGTGATAGTCAGTCACTATGGAGATCGCGGATGGTAACGTc                                                              <  1:350495/140‑1 (MQ=255)                                                                        tCACTGAAATGGTGTTTTATAGTTAAATATAAGTAAATATATTGTTGCAATAAATGCGAGATCTGTGGTACTTATTAAGTAGCAGCGGAAGTTCCCGGCAGTGATAGTCAGTCACTATGGAGATCGCGGATGGTAACGTc                                                              <  1:1184640/140‑1 (MQ=255)                                                                        tCACTGAAATGGTGTTTTATAGTTAAATATAAGTAAATATATTGTTGCAATAAATGCAAGATCTGTGGTACTTATTAAGTAGCAGCGGAAGTTCCCGGCAGTGATAGTCAGTCACTATGGAGATCGCGGATGGTAACGTc                                                              <  1:1098969/140‑1 (MQ=255)                                                                                   gtgtTTTATAGTTAAATATAAGTAAATATATTGTTGCAATAAATGCGAGATCTGTGGTACTTATTAAGTAGCAGCGGAAGTTCCCGGCAGTGATAGTCAGTCACTATGGAGATCGCGGATGGTAACGTCCTGTACTGGAc                                                   <  2:734935/140‑1 (MQ=255)                                                                                         tataGTTAAATATAAGTAAATATATTGTTGCAATAAATGCGAGATCTGTGGTACTTATTAAGTAGCAGCGGAAGTTCCCGGCAGTGATAGTCAGTCACTATGGAGATCGCGGATGGTAACGTCCTGTACTGGACATGttt                                             >  1:197087/1‑140 (MQ=255)                                                                                                        gTAAATATATTGTTGCAATAAATGCGAGATCTGTGGTACTTATTAAGTAGCAGCGGAAGTTCCCGGCAGTGATAGTCAGTCACTATGGAGATCGCGGATGGTAACGTCCTGTACTGGACATGTTTTAGATAATCAAcgcg                              >  2:1187183/1‑140 (MQ=255)                                                                                                                atTGTTGCAATAAATGCGAGATCTGTGGTACTTATTAAGTAGCAGCGGAAGTTCCCGGCAGTGATAGCCAGTCACTATGGAGATCGCGGATGGTAACGTCCTGTACTGGACATGTTTTAGATAATCAACGCGCCACCACt                      >  2:862873/1‑140 (MQ=255)                                                                                                                 ttgttgCAATAAATGCGAGATCTGTGGTACTTATTAAGTAGCAGCGGAAGTTCCCGGCAGTGATAGTCAGTCACTATGGAGATCGCGGATGGTAACGTCCTGTACTGGACATGTTTTAGATAATCAACGCGCCACCACTc                     >  1:1168250/1‑140 (MQ=255)                                                                                                                   gttgCAATAAATGCGAGATCTGTGGTACTTATTAAGTAGCAGCGGAAGTTCCCGGCAGTGATAGTCAGTCACTATGGAGATCGCGGATGGTAACGTCCTGTACTGGACATGTTTTAGATAATCAACGCGCCACCACTcgc                   >  1:1137486/1‑140 (MQ=255)                                                                                                                    ttgCAATAAATGCGAGATCTGTGGTACTTATTAAGTAGCAGCGGAAGTTCCCGGCAGTGATAGTCAGTCACTATGGAGATCGCGGATGGTAACGTCCTGTACTGGACATGTTTTAGATAATCAACGCGCCACCACTcgcg                  <  2:1168250/140‑1 (MQ=255)                                                                                                                       cAATAAATGCGAGATCTGTGGTACTTATTAAGTAGCAGCGGAAGTTCCCGGCAGTGATAGTCAGTCACTATGGAGATCGCGGATGGTAACGTCCTGTACTGGACATGTTTTAGATAATCAACGCGCCACCACTCGCGGAg               >  2:40011/1‑140 (MQ=255)                                                                                                                         aTAAATGCGAGATCTGTGGTACTTATTAAGTAGCAGCGGAAGTTCCCGGCAGTGATAGTCAGTCACTATGGAGATCGCGGATGGTAACGTCCTGTACTGGACATGTTTTAGATAATCAACGCGCCACCACTCGCGGAGtt             >  2:770050/1‑140 (MQ=255)                                                                                                                                  agaTCTGTGGTACTTATTAAGTAGCAGCGGAAGTTCCCGGCAGTGATAGTCAGTCACTATGGAGATCGCGGATGGTAACGTCCTGTACTGGACATGTTTTAGATAATCAACGCGCCACCACTCGCGGAGTTTTCtcctcc    >  2:260841/1‑140 (MQ=255)                                                                                                                                   gaTCTGTGGTACTTATTAAGTAGCAGCGGAAGTTCCCGGCAGTGATAGTCAGTCACTATGGAGATCGCGGATGGTAACGTCCTGTACTGGACATGTTTTAGATAATCAACGCGCCACCACTCGCGGAGTTTTCTCCTCCg   >  2:630864/1‑140 (MQ=255)                                                                                                                                    aTCTGTGGTACTTATTAAGTAGCAGCGGAAGTTCCCGGCAGTGATAGTCAGTCACTATGGAGATCGCGGATGGTAACGTCCTGTACTGGACATGTTTTAGATAATCAACGCGCCACCACTCGCGGAGTTTTCTCCTCCgg  <  2:768419/140‑1 (MQ=255)                                                                                                                                      cTGTGGTACTTATTAAGTAGCAGCGGAAGTTCCCGGCAGTGATAGTCAGTCACTATGGAGATCGCGGATGGTAACGTCCTGTACTGGACATGTTTTAGATAATCAACGCGCCACCACTCGCGGAGTTTtctc        >  1:825652/1‑132 (MQ=255)                                                                                                                                      cTGTGGTACTTATTAAGTAGCAGCGGAAGTTCCCGGCAGTGATAGTCAGTCACTATGGAGATCGCGGATGGTAACGTCCTGTACTGGACATGTTTTAGATAATCAACGCGCCACCACTCGCGGAGTTTtctc        <  2:825652/132‑1 (MQ=255)                                                                                                                                          |                                                                                                                                      AATCCTGCTGAAAGCACACAGCTTTTTTCATCACTGTCATCACTCTGTCATCTTTCCAGTAGAAACTAATGTCACTGAAATGGTGTTTTATAGTTAAATATAAGTAAATATATTGTTGCAATAAATGCGAGATCTGTTGTACTTATTAAGTAGCAGCGGAAGTTCCCGGCAGTGATAGTCAGTCACTATGGAGATCGCGGATGGTAACGTCCTGTACTGGACATGTTTTAGATAATCAACGCGCCACCACTCGCGGAGTTTTCTCCTCCGG  >  NC_000913/1084792‑1085062

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑