Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,230,319	G→T	L26I (CTT→ATT)	hlyE ←	hemolysin E

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,230,319	0	G	T	100.0%	180.8 / NA	51	L26I (CTT→ATT)	hlyE	hemolysin E
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (23/28);  total (23/28)

ATAAGTAAGGTTTTAATATCGCCGACTAAAACGGAGGCTGCCTGTGAATACTCCTGTTTAAAGCGACTTAACTCTTTTATGGTTTCATCAAAGGTCTGCCAGGGGATGACCTGATCGAGATATTTATTATAAAGATCTAATGCTCCATCTGCGGTTTCGATTGCGTTTTTAACTACTTCTACCGTTTTATCTGCAACGATTTCAGTCATAATCATTCGCCTCTTTAAATATATAAATTGTAATGAAACTCCTGTTTTACAACTATTAATA  >  NC_000913/1230186‑1230455                                                                                                                                      |                                                                                                                                         aTAAGTAAGGTTTTAATATCGCCGACTAAAACGGAGGCTGCCTGTGAATACTCCTGTTTAAAGCGACTTAACTCTTTTATGGTTTCATCAAAGGTCTGCCAGGGGATGACCTGATCGAGATATTTATTATAAATATCTaa                                                                                                                                    <  1:1038687/140‑1 (MQ=255)        agGTTTTAATATCGCCGACTAAAACGGAGGCTGCCTGTGAATACTCCTGTTTAAAGCGACTTAACTCTTTTATGGTTTCATCAAAGGTCTGCCAGGGGATGACCTGATCGAGATATTTATTATAAATATc                                                                                                                                       >  2:554689/1‑130 (MQ=255)        agGTTTTAATATCGCCGACTAAAACGGAGGCTGCCTGTGAATACTCCTGTTTAAAGCGACTTAACTCTTTTATGGTTTCATCAAAGGTCTGCCAGGGGATGACCTGATCGAGATATTTATTATAAATATc                                                                                                                                       <  1:554689/130‑1 (MQ=255)        agGTTTTAATATCGCCGACTAAAACGGAGGCTGCCTGTGAATACTCCTGTTTAAAGCGACTTAACTCTTTTATGGTTTCATCAAAGGTCTGCCAGGGGATGACCTGATCGAGATATTTATTATAAATATCTAATGCTCCa                                                                                                                             >  1:717889/1‑140 (MQ=255)                atatCGCCGACTAAAACGGAGGCTGCCTGTGAATACTCCTGTTTAAAGCGACTTAACTCTTTTATGGTTTCATCAAAGGTCTGCCAGGGGATGACCTGATCGAGATATTTATTATAAATATCTAATGCTCCATCTGCGGt                                                                                                                     >  1:290608/1‑140 (MQ=255)                   tCGCCGACTAAAACGGAGGCTGCCTGTGAATACTCCTGTTTAAAGCGACTTAACTCTTTTATGGTTTCATCAAAGGTCTGCCAGGGGATGACCTGATCGAGATATTTATTATAAATATCTAATGCTCCATCt                                                                                                                          <  2:902252/132‑1 (MQ=255)                   tCGCCGACTAAAACGGAGGCTGCCTGTGAATACTCCTGTTTAAAGCGACTTAACTCTTTTATGGTTTCATCAAAGGTCTGCCAGGGGATGACCTGATCGAGATATTTATTATAAATATCTAATGCTCCATCt                                                                                                                          >  1:902252/1‑132 (MQ=255)                                cGGAGGCTGCCTGTGAATACTCCTGTTTAAAGCGACTTAACTCTTTTATGGTTTCATCAAAGGTCTGCCAGGGGATGACCTGATCGAGATATTTATTATAAATATCTAATGCTCCATCTGCGGTTTCGATTGCGTTTTTa                                                                                                     <  2:401605/140‑1 (MQ=255)                                    ggCTGCCTGTGAATACTCCTGTTTAAAGCGACTTAACTCTTTTATGGTTTCATCAAAGGTCTGCCAGGGGATGACCTGATCGAGATATTTATTATAAATATCTAATGCTCCATCTGCGGTTTCGATTGCGTTTTTAacta                                                                                                 >  1:725603/1‑140 (MQ=255)                                        gCCTGTGAATACTCCTGTTTAAAGCGACTTAACTCTTTTATGGTTTCATCAAAGGTCTGCCAGGGGATGACCTGATCGAGATATTTATTATAAATATCTAATGCTCCATCTGCGGTTTCGATTGCGTTTTTAACTACTTc                                                                                             >  1:1088227/1‑140 (MQ=255)                                         ccTGTGAATACTCCTGTTTAAAGCGACTTAACTCTTTTATGGTTTCATCAAAGGTCTGCCAGGGGATGACCTGATCGAGATATTTATTATAAATATCTAATGCTCCATCTGCGGTTTCGATTGCGTTTTTa                                                                                                     <  2:516933/131‑1 (MQ=255)                                         ccTGTGAATACTCCTGTTTAAAGCGACTTAACTCTTTTATGGTTTCATCAAAGGTCTGCCAGGGGATGACCTGATCGAGATATTTATTATAAATATCTAATGCTCCATCTGCGGTTTCGATTGCGTTTTTa                                                                                                     >  1:516933/1‑131 (MQ=255)                                               aaTACTCCTGTTTAAAGCGACTTAACTCTTTTATGGTTTCATCAAAGGTCTGCCAGGGGATGACCTGATCGAGATATTTATTATAAATATCTAATGCTCCATCTGCGGTTTCGATTGCGTTTTTAACTACTTCTACCGtt                                                                                      >  2:751028/1‑140 (MQ=255)                                                    tCCTGTTTAAAGCGACTTAACTCTTTTATGGTTTCATCAAAGGTCTGCCAGGGGATGACCTGATCGAGATATTTATTATAAATATCTAATGCTCCATCTGCGGTTTCGATTGCGTTTTTAACTACTTCTACCGTTTTATc                                                                                 >  2:939608/1‑140 (MQ=255)                                                    tCCTGTTTAAAGCGACTTAACTCTTTTATGGTTTCATCAAAGGTCTGCCAGGGGATGACCTGATCGAGATATTTATTATAAATATCTAATGCTCCATCTGCGGTTTCGATTGCGTTTTTAACTACTTCTACCGTTTTATc                                                                                 >  2:1163427/1‑140 (MQ=255)                                                      cTGTTTAAAGCGACTTAACTCTTTTATGGTTTCATCAAAGGTCTGCCAGGGGATGACCTGATCGAGATATTTATTATAAATATCTAATGCTCCATCTGCGGTTTCGATTGCGTTTTTAACTACTTCTACCGTTTTATCTg                                                                               >  2:602071/1‑140 (MQ=255)                                                       tGTTTAAAGCGACTTAACTCTTTTATGGTTTCATCAAAGGTCTGCCAGGGGATGACCTGATCGAGATATTTATTATAAATATCTAATGCTCCATCTGCGGTTTCGATTGCGTTTTTAACTACTTCTACCGTTTTATCTGc                                                                              <  1:590615/140‑1 (MQ=255)                                                         tttAAAGCGACTTAACTCTTTTATGGTTTCATCAAAGGTCTGCCAGGGGATGACCTGATCGAGATATTTATTATAAATATCTAATGCTCCATCTGCGGTTTCGATTGCGTTTTTAACTACTTCTACCGTTTTATCTGCaa                                                                            >  2:868510/1‑140 (MQ=255)                                                         tttAAAGCGACTTAACTCTTTTATGGTTTCATCAAAGGTCTGCCAGGGGATGACCTGATCGAGATATTTATTATAAATATCTAATGCTCCATCTGCGGTTTCGATTGCGTTTTTAACTACTTCTACCGTTTTATCTGCaa                                                                            >  1:128646/1‑140 (MQ=255)                                                              aGCGACTTAACTCTTTTATGGTTTCATCAAAGGTCTGCCAGGGGATGACCTGATCGAGATATTTATTATAAATATCTAATGCTCCATCTGCGGTTTCGATTGCGTTTTTAACTACTTCTACCGTTTTATCTGCAACGAtt                                                                       >  2:1138309/1‑140 (MQ=255)                                                               gCGACTTAACTCTTTTATTGTTTCATCAAAGGTCTGCCAGGGGATGACCTGATCGAGATATTTATTATAAATATCTAATGCTCCATCTGCGGTTTCGATTGCGTTTTTAACTACTTCTACCGTTTTATCTGCAACGAttt                                                                      <  1:604019/140‑1 (MQ=255)                                                               gCGACTTAACTCTTTTATGGTTTCATCAAAGGTCTGCCAGGGGATGACCTGATCGAGATATTTATTATAAATATCTAATGCTCCATCTGCGGTTTCGATTGCGTTTTTAACTACTTCTACCGTTTTATCTGCAACGAttt                                                                      <  2:128646/140‑1 (MQ=255)                                                                 gACTTAACTCTTTTATGGTTTCATCAAAGGTCTGCCAGGGGATGACCTGATCGAGATATTTATTATAAATATCTAATGCTCCATCTGCGGTTTCGATTGCGTTTTTAACTACTTCTACCGTTTTATCTGCAACGATTTCa                                                                    <  1:697985/140‑1 (MQ=255)                                                                   cTTAACTCTTTTATGGTTTCATCAAAGGTCTGCCAGGGGATGACCTGATCGAGATATTTATTATAAATATCTAATGCTCCATCTGCGGTTTCGATTGCGTTTTTAACTACTTCTACCGTTTTATCTGCAACGATTTCAGt                                                                  >  2:335816/1‑140 (MQ=255)                                                                   cTTAACTCTTTTATGGTTTCATCAAAGGTCTGCCAGGGGATGACCTGATCGAGATATTTATTATAAATATCTAATGCTCCATCTGCGGTTTCGATTGCGTTTTTAACTACTTCTACCGTTTTATCTGCAACGATTTCAGt                                                                  >  2:424979/1‑140 (MQ=255)                                                                   cTTAACTCTTTTATGGTTTCATCAAAGGTCTGCCAGGGGATGACCTGATCGAGATATTTATTATAAATATCTAATGCTCCATCTGCGGTTTCGATTGCGTTTTTAACTACTTCTACCGTTTTATCTGCAACGATTTCAGt                                                                  >  2:414508/1‑140 (MQ=255)                                                                   cTTAACTCTTTTATGGTTTCATCAAAGGTCTGCCAGGGGATGACCTGATCGAGATATTTATTATAAATATCTAATGCTCCATCTGCGGTTTCGATTGCGTTTTTAACTACTTCTACCGTTTTATCTGCAACGATTTCAGt                                                                  <  1:560353/140‑1 (MQ=255)                                                                      aaCTCTTTTATGGTTTCATCAAAGGTCTGCCAGGGGATGACCTGATCGAGATATTTATTATAAATATCTAATGCTCCATCTGCGGTTTCGATTGCGTTTTTAACTACTTCTACCGTTTTATCTGCAACGATTTCAGTCAt                                                               <  1:1163427/140‑1 (MQ=255)                                                                             ttATGGTTTCATCAAAGGTCTGCCAGGGGATGACCTGATCGAGATATTTATTATAAATATCTAATGCTCCATCTGCGGTTTCGATTGCGTTTTTAACTACTTCTACCGTTTTATCTGCAACGATTTCAGTCATAATCAtt                                                        <  1:1138309/140‑1 (MQ=255)                                                                             ttATGGTTTCATCAAAGGTCTGCCAGGGGATGACCTGATCGAGATATTTATTATAAATATCTAATGCTCCATCTGCGGTTTCGATTGCGTTTTTAACTACTTCTACCGTTTTATCTGCAACGATTTCAGTCATAATCAtt                                                        >  1:735876/1‑140 (MQ=255)                                                                               aTGGTTTCATCAAAGGTCTGCCAGGGGATGACCTGATCGAGATATTTATTATAAATATCTAATGCTCCATCTGCGGTTTCGATTGCGTTTTTAACTACTTCTACCGTTTTATCTGCAACGATTTCAGTCATAATCATTCg                                                      <  1:335816/140‑1 (MQ=255)                                                                                tGGTTTCATCAAAGGTCTGCCAGGGGATGACCTGATCGAGATATTTATTATAAATATCTAATGCTCCATCTGCGGTTTCGATTGCGTTTTTAACTACTTCTACCGTTTTATCTGCAACGATTTCAGTCATAATCATTCGc                                                     <  2:290608/140‑1 (MQ=255)                                                                                tGGTTTCATCAAAGGTCTGCCAGGGGATGACCTGATCGAGATATTTATTATAAATATCTAATGCTCCATCTGCGGTTTCGATTGCGTTTTTAACTACTTCTACCGTTTTATCTGCAACGATTTCAGTCATAATCATTCGc                                                     <  1:751028/140‑1 (MQ=255)                                                                                      catcaAAGGTCTGCCAGGGGATGACCTGATCGAGATATTTATTATAAATATCTAATGCTCCATCTGCGGTTTCGATTGCGTTTTTAACTACTTCTACCGTTTTATCTGCAACGATTTCAGTCATAATCATTCGCCTCttt                                               <  1:868510/140‑1 (MQ=255)                                                                                       atcaAAGGTCTGCCAGGGGATGACCTGATCGAGATATTTATTATAAATATCTAATGCTCCATCTGCGGTTTCGATTGCGTTTTTAACTACTTCTACCGTTTTATCTGCAACGATTTCAGTCATAATCATTCGCCTCTTTa                                              <  1:1029203/140‑1 (MQ=255)                                                                                         caAAGGTCTGCCAGGGGATGACCTGATCGAGATATTTATTATAAATATCTAATGCTCCATCTGCGGTTTCGATTGCGTTTTTAACTACTTCTACCGTTTTATCTGCAACGATTTCAGTCATAATCATTCGCCTCTTTaaa                                            <  2:326975/140‑1 (MQ=255)                                                                                         caAAGGTCTGCCAGGGGATGACCTGATCGAGATATTTATTATAAATATCTAATGCTCCATCTGCGGTTTCGATTGCGTTTTTAACTACTTCTACCGTTTTATCTGCAACGATTTCAGTCATAATCATTCGCCTCTTTaaa                                            <  1:602071/140‑1 (MQ=255)                                                                                                    cAGGGGATGACCTGATCGAGATATTTATTATAAATATCTAATGCTCCATCTGCGGTTTCGATTGCGTTTTTAACTACTTCTACCGTTTTATCTGCAACGATTTCAGTCATAATCATTCGCCTCTTTAAATATATAAATTg                                 <  2:735876/140‑1 (MQ=255)                                                                                                       gggATGACCTGATCGAGATATTTATTATAAATATCTAATGCTCCATCTGCGGTTTCGATTGCGTTTTTAACTACTTCTACCGTTTTATCTGCAACGATTTCAGTCATAATCATTCGCCTCTTTAAATATATAAATTGTaa                              <  2:717889/140‑1 (MQ=255)                                                                                                         gATGACCTGATCGAGATATTTATTATAAATATCTAATGCTCCATCTGCGGTTTCGATTGCGTTTTTAACTACTTCTACCGTTTTATCTGCAACGATTTCAGTCATAATCATTCGCCTCTTTa                                              >  1:846014/1‑122 (MQ=255)                                                                                                         gATGACCTGATCGAGATATTTATTATAAATATCTAATGCTCCATCTGCGGTTTCGATTGCGTTTTTAACTACTTCTACCGTTTTATCTGCAACGATTTCAGTCATAATCATTCGCCTCTTTa                                              <  2:846014/122‑1 (MQ=255)                                                                                                             aCCTGATCGAGATATTTATTATAAATATCTAATGCTCCATCTGCGGTTTCGATTGCGTTTTTAACTACTTCTACCGTTTTATCTGCAACGATTTCAGTCATAATCATTCGCCTCTTTAAATATATAAATTGTAATGAAAc                        >  1:280209/1‑140 (MQ=255)                                                                                                                tGATCGAGATATTTATTATAAATATCTAATGCTCCATCTGCGGTTTCGATTGCGTTTTTAACTACTTCTACCGTTTTATCTGCAACGATTTCAGTCATAATCATTCGCCTCTTTAAATATATAAATTGTAATGAAACTcc                     <  1:422781/140‑1 (MQ=255)                                                                                                                tGATCGAGATATTTATTATAAATATCTAATGCTCCATCTGCGGTTTCGATTGCGTTTTTAACTACTTCTACCGTTTTATCTGCAACGATTTCAGTCATAATCATTCGCCTCTTTAAATATATAAATTGTAATGAAACTcc                     <  1:237284/140‑1 (MQ=255)                                                                                                                  aTCGAGATATTTATTATAAATATCTAATGCTCCATCTGCGGTTTCGATTGCGTTTTTAACTACTTCTACCGTTTTATCTGCAACGATTTCAGTCATAATCATTCGCCTCTTTAAATATATAAATTGTAATGAAACTcc                     >  1:203609/1‑138 (MQ=255)                                                                                                                  aTCGAGATATTTATTATAAATATCTAATGCTCCATCTGCGGTTTCGATTGCGTTTTTAACTACTTCTACCGTTTTATCTGCAACGATTTCAGTCATAATCATTCGCCTCTTTAAATATATAAATTGTAATGAAACTcc                     <  2:203609/138‑1 (MQ=255)                                                                                                                   tCGAGATATTTATTATAAATATCTAATGCTCCATCTGCGGTTTCGATTGCGTTTTTAACTACTTCTACCGTTTTATCTGCAACGATTTCAGTCATAATCATTCGCCTCTTTAAATATATAAATTGTAATGAAACTCCTGt                  >  2:55479/1‑140 (MQ=255)                                                                                                                        atatTTATTATAAATATCTAATGCTCCATCTGCGGTTTCGATTGCGTTTTTAACTACTTCTACCGTTTTATCTGCAACGATTTCAGTCATAATCATTCGCCTCTTTAAATATATAAATTGTAATGAAACTCCTGTTTTAc             <  2:725603/140‑1 (MQ=255)                                                                                                                                 ataAATATCTAATGCTCCATCTGCGGTTTCGATTGCGTTTTTAACTACTTCTACCGTTTTATCTGCAACGATTTCAGTCATAATCATTCGCCTCTTTAAATATATAAATTGTAATGAAACTCCTGTTTTACAACTATtaa    >  2:651441/1‑140 (MQ=255)                                                                                                                                  taAATATCTAATGCTCCATCTGCGGTTTCGATTGCGTTTTTAACTACTTCTACCGTTTTATCTGCAACGATTTCAGTCATAACCATTCGCCTCTTTATATATATAAATTGTAATGAAACTCCTGTTTTACAACTATtaat   <  2:936515/140‑1 (MQ=255)                                                                                                                                   aaaTATCTAATGCTCCATCTGCGGTTTCGATTGCGTTTTTAACTACTTCTACCGTTTTATCTGCAACGATTTCAGTCATAATCATTCGCCTCTTTAAATATATAAATTGTAATGAAACTCCTGTTTTACAACTATtaata  <  1:55479/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                                         ATAAGTAAGGTTTTAATATCGCCGACTAAAACGGAGGCTGCCTGTGAATACTCCTGTTTAAAGCGACTTAACTCTTTTATGGTTTCATCAAAGGTCTGCCAGGGGATGACCTGATCGAGATATTTATTATAAAGATCTAATGCTCCATCTGCGGTTTCGATTGCGTTTTTAACTACTTCTACCGTTTTATCTGCAACGATTTCAGTCATAATCATTCGCCTCTTTAAATATATAAATTGTAATGAAACTCCTGTTTTACAACTATTAATA  >  NC_000913/1230186‑1230455

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑