Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,205,858	A→T	D133E (GAT→GAA)	ttdR ←	DNA‑binding transcriptional activator Dan

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,205,858	0	A	T	100.0%	159.2 / NA	45	D133E (GAT→GAA)	ttdR	DNA‑binding transcriptional activator Dan
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (27/18);  total (27/18)

TTTTTGCAGATACTCAGGTGCTGCGCATAATATTCTTTTATTTTTTGTCAAAAGATGCGCAATATAATAATCAGGAATTTCGTCATTAATACGAATATCCAGATCAATATTATCCTGCACTAAATCAATTTGCCGATCGAACAATTCAAAATGCACCTGTAACTCAGGATAATTGCGCATCAGTTCGGTAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATACGAATCATCCCCTCCGGGCGCGTTTTGAT  >  NC_000913/3205723‑3205989                                                                                                                                        |                                                                                                                                    tttttGCAGATACTCAGGTGCTGCGCATAATATTCTTTTATTTTTTGTCAAAAGATGCGCAATATAATAATCAGGAATTTCGTCATTAATACGAATATCCAGATCAATATTATCCTGCACTAAATCAATTTGCCGTTCGa                                                                                                                                 <  2:78754/140‑1 (MQ=255)       cAGATACTCAGGTGCTGCGCATAATATTCTTTTATTTTTTGTCAAAAGATGCGCAATATAATAATCAGGAATTTCGTCATTAATACGAATATCCAGATCAATATTATCCTGCACTAAATCAATTTGCCGTTCGAACAAtt                                                                                                                           <  2:699606/140‑1 (MQ=255)             cTCAGGTGCTGCGCATAATATTCTTTTATTTTTTGTCAAAAGATGCGCAATATAATAATCAGGAATTTCGTCATTAATACGAATATCCAGATCAATATTATCCTGCACTAAATCAATTTGCCGTTCGAACAATTCAAAAt                                                                                                                     <  1:332288/140‑1 (MQ=255)                   tgctgcGCATAATATTCTTTTATTTTTTGTCAAAAGATGCGCAATATAATAATCAGGAATTTCGTCATTAATACGAATATCCAGATCAATATTATCCTGCACTAAATCAATTTGCCGTTCGAACAATTCAAAATGCACCt                                                                                                               >  1:90781/1‑140 (MQ=255)                      tgcGCATAATATTCTTTTATTTTTTGTCAAAAGATGCGCAATATAATAATCAGGAATTTCGTCATTAATACGAATATCCAGATCAATATTATCCTGCACTAAATCAATTTGCCGTTCGAACAATTCAAAATGCACCTGTa                                                                                                            >  1:563278/1‑140 (MQ=255)                        cgcATAATATTCTTTTATTTTTTGTCAAAAGATGCGCAATATAATAATCAGGAATTTCGTCATTAATACGAATATCCAGATCAATATTATCCTGCACTAAATCAATTTGCCGTTCGAACAATTCAAAATGCACCTGTAAc                                                                                                          >  2:751746/1‑140 (MQ=255)                         gcATAATATTCTTTTATTTTTTGTCAAAAGATGCGCAATATAATAATCAGGAATTTCGTCATTAATACGAATATCCAGATCAATATTATCCTGCACTAAATCAATTTGCCGTTCGAACAATTCAAAATGCACCTGTAACt                                                                                                         <  2:563278/140‑1 (MQ=255)                                  tCTTTTATTTTTTGTCAAAAGATGCGCAATATAATAATCAGGAATTTCGTCATTAATACGAATATCCAGATCAATATTATCCTGCACTAAATCAATTTGCCGTTCGAACAATTCAAAATGCACCTGTAACTCAGGATAAt                                                                                                >  1:244635/1‑140 (MQ=255)                                     tttATTTTTTGTCAAAAGATGCGCAATATAATAATCAGGAATTTCGTCATTAATACGAATATCCAGATCAATATTATCCTGCACTAAATCAATTTGCCGTTCGAACAATTCAAAATGCACCTGTAACTCAGGATAATTgc                                                                                             >  1:104436/1‑140 (MQ=255)                                         ttttttGTCAAAAGATGCGCAATATAATAATCAGGAATTTCGTCATTAATACGAATATCCAGATCAATATTATCCTGCACTAAATCAATTTGCCGTTCGAACAATTCAAAATGCACCTGTAACTCTGGATAATTGCGCAt                                                                                         >  2:685849/1‑140 (MQ=255)                                              tGTCAAAAGATGCGCAATATAATAATCAGGAATTTCGTCATTAATACGAATATCCAGATCAATATTATCCTGCACTAAATCAATTTGCCGTTCGAACAATTCAAAATGCACCTGTAACTCAGGATAATTGCGCATCAGtt                                                                                    <  2:660738/140‑1 (MQ=255)                                                          cgcAATATAATAATCAGGAATTTCGTCATTAATACGAATATCCAGATCAATATTATCCTGCACTAAATCAATTTGCCGTTCGAACAATTCAAAATGCACCTGTAACTCAGGATAATTGCGCATCAGTTCGGTAATAGCTg                                                                        >  2:89981/1‑140 (MQ=255)                                                             aaTATAATAATCAGGAATTTCGTCATTAATACGAATATCCAGATCAATATTATCCTGCACTAAATCAATTTGCCGTTCGAACAATTCAAAATGCACCTGTAACTCAGGATAATTGCGCATCAGTTCGGTAATAGCTGgcg                                                                     <  1:751746/140‑1 (MQ=255)                                                                 taataaTCAGGAATTTCGTCATTAATACGAATATCCAGATCAATATTATCCTGCACTAAATCAATTTGCCGTTCGAACAATTCAAAATGCACCTGTAACTCAGGATATTTGCGCATCAGTTCGGTAATAGCTGGCGCAat                                                                 >  1:451571/1‑140 (MQ=255)                                                                         aGGAATTTCGTCATTAATACGAATATCCAGATCAATATTATCCTGCACTAAATCAATTTGCCGTTCGAACAATTCAAAATGCACCTGTAACTCAGGATAATTGCGCATCAGTTCGGTAATAGCTGGCGCAATATGGCTgc                                                         >  2:715337/1‑140 (MQ=255)                                                                            aaTTTCGTCATTAATACGAATATCCAGATCAATATTATCCTGCACTAAATCAATTTGCCGTTCGAACAATTCAAAATGCACCTGTAACTCAGGATAATTGCGCATCAGTTCGGTAATAGCTGGCGCAATATGGCTGCGcc                                                      <  1:715337/140‑1 (MQ=255)                                                                             aTTTCGTCATTAATACGAATATCCAGATCAATATTATCCTGCACTAAATCAATTTGCCGTTCGAACAATTCAAAATGCACCTGTAACTCAGGATAATTGCGCATCAGTTCGGTAATAGCTGGCGCAATATGGCTGCGccc                                                     <  2:105183/140‑1 (MQ=255)                                                                                            cGAATATCCAGATCAATATTATCCTGCACTAAATCAATTTGCCGTTCGAACAATTCAAAATGCACCTGTAACTCAGGATAATTGCGCATCAGTTCGGTAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCa                                      <  1:264626/140‑1 (MQ=255)                                                                                            cGAATATCCAGATCAATATTATCCTGCACTAAATCAATTTGCCGTTCGAACAATTCAAAATGCACCTGTAACTCAGGATAATTGCGCATCAGTTCGGTAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCa                                      <  1:89981/140‑1 (MQ=255)                                                                                              aaTATCCAGATCAATATTATCCTGCACTAAATCAATTTGCCGTTCGAACAATTCAAAATGCACCTGTAACTCAGGATAATTGCGCATCAGTTCGGTAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAAc                                    <  2:90781/140‑1 (MQ=255)                                                                                                       aTCAATATTATCCTGCACTAAATCAATTTGCCGTTCGAACAATTCAAAATGCACCTGTAACTCAGGATAATTGCGCATCAGTTCGGTAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATACGaa                           >  1:608267/1‑140 (MQ=255)                                                                                                       aTCAATATTATCCTGCACTAAATCAATTTGCCGTTCGAACAATTCAAAATGCACCTGTAACTCAGGATAATTGCGCATCAGTTCGGTAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATACGaa                           >  1:130393/1‑140 (MQ=255)                                                                                                       aTCAATATTATCCTGCACTAAATCAATTTGCCGTTCGAACAATTCAAAATGCACCTGTAACTCAGGATAATTGCGCATCAGTTCGGTAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATACGaa                           >  1:238024/1‑140 (MQ=255)                                                                                                         cAATATTATCCTGCACTAAATCAATTTGCCGTTCGAACAATTCAAAATGCACCTGTAACTCAGGATAATTGCGCATCAGTTCGGTAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATACGAatc                         >  2:109770/1‑140 (MQ=255)                                                                                                            tattatCCTGCACTAAATCAATTTGCCGTTCGAACAATTCAAAATGCACCTGTAACTCAGGATAATTGCGCATCAGTTCGGTAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCa                                      <  1:548481/124‑1 (MQ=255)                                                                                                            tattatCCTGCACTAAATCAATTTGCCGTTCGAACAATTCAAAATGCACCTGTAACTCAGGATAATTGCGCATCAGTTCGGTAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCa                                      >  2:548481/1‑124 (MQ=255)                                                                                                            tattatCCTGCACTAAATCAATTTGCCGTTCGAACAATTCAAAATGCACCTGTAACTCAGGATAATTGCGCATCAGTTCGGTAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATACGAatcatc                      >  1:344896/1‑140 (MQ=255)                                                                                                                 tCCTGCACTAAATCAATTTGCCGTTCGAACAATTCAAAATGCACCTGTAACTCAGGATAATTGCGCATCAGTTCGGTAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATACGa                            <  1:738625/129‑1 (MQ=255)                                                                                                                 tCCTGCACTAAATCAATTTGCCGTTCGAACAATTCAAAATGCACCTGTAACTCAGGATAATTGCGCATCAGTTCGGTAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATACGa                            >  2:738625/1‑129 (MQ=255)                                                                                                                 tCCTGCACTAAATCAATTTGCCGTTCGAACAATTCAAAATGCACCTGTAACTCAGGATAATTGCGCATCAGTTCGGTAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATACGAATCATCCCCTc                 >  1:303886/1‑140 (MQ=255)                                                                                                                 tCCTGCACTAAATCAATTTGCCGTTCGAACAATTCAAAATGCACCTGTAACTCAGGATAATTGCGCATCAGTTCGGTAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATACGAATCATCCCCTc                 >  2:83767/1‑140 (MQ=255)                                                                                                                   cTGCACTAAATCAATTTGCCGTTCGAACAATTCAAAATGCACCTGTAACTCAGGATAATTGCGCATCAGTTCGGTAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATACGAATCATCCCCTCCg               >  2:323746/1‑140 (MQ=255)                                                                                                                    tGCACTAAATCAATTTGCCGTTCGAACAATTCAAAATGCACCTGTAACTCAGGATAATTGCGCATCAGTTCGGTAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATACGAATCATCCCCTCCgg              >  2:132976/1‑140 (MQ=255)                                                                                                                    tGCACTAAATCAATTTGCCGTTCGAACAATTCAAAATGCACCTGTAACTCAGGATAATTGCGCATCAGTTCGGTAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATACGAATCATCCCCTCCgg              >  1:601889/1‑140 (MQ=255)                                                                                                                      cACTAAATCAATTTGCCGTTCGAACAATTCAAAATGCACCTGTAACTCAGGATAATTGCGCATCAGTTCGGTAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATACGAatca                        >  2:543466/1‑128 (MQ=255)                                                                                                                      cACTAAATCAATTTGCCGTTCGAACAATTCAAAATGCACCTGTAACTCAGGATAATTGCGCATCAGTTCGGTAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATACGAatca                        <  1:543466/128‑1 (MQ=255)                                                                                                                      cACTAAATCAATTTGCCGTTCGAACAATTCAAAATGCACCTGTAACTCAGGATAATTGCGCATCAGTTCGGTAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATACGAATCATccc                    <  2:161416/132‑1 (MQ=255)                                                                                                                      cACTAAATCAATTTGCCGTTCGAACAATTCAAAATGCACCTGTAACTCAGGATAATTGCGCATCAGTTCGGTAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATACGAATCATccc                    >  1:161416/1‑132 (MQ=255)                                                                                                                      cACTAAATCAATTTGCCGTTCGAACAATTCAAAATGCACCTGTAACTCAGGATAATTGCGCATCAGTTCGGTAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATACGAATCATCCCCTCCGGgc            <  2:608267/140‑1 (MQ=255)                                                                                                                      cACTAAATCAA‑TTGCCGTTCGAACAATTCAAAATGCACCTGTAACTCAGGATAATTGCGCATCAGTTCGGTAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATACGAATCATCCCCTCCGGgcg           >  1:20046/1‑140 (MQ=255)                                                                                                                         tAAATCAATTTGCCGTTCGAACAATTCAAAATGCACCTGTAACTCAGGATAATTGCGCATCAGTTCGGTAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATACGAatca                        <  2:202197/125‑1 (MQ=255)                                                                                                                         tAAATCAATTTGCCGTTCGAACAATTCAAAATGCACCTGTAACTCAGGATAATTGCGCATCAGTTCGGTAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATACGAatca                        >  1:202197/1‑125 (MQ=255)                                                                                                                           aaTCAATTTGCCGTTCGAACAATTCAAAATGCACCTGTAACTCAGGATAATTGCGCATCAGTTCGGTAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATACGAATCATCCCCTCCGGGCGCGtt       >  1:473994/1‑140 (MQ=255)                                                                                                                           aaTCAATTTGCCGTTCGAACAATTCAAAATGCACCTGTAACTCAGGATAATTGCGCATCAGTTCGGTAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATACGAATCATCCCCTCCGGGCGCGtt       >  1:125806/1‑140 (MQ=255)                                                                                                                                aTTTGCCGTTCGAACAATTCAAAATGCACCTGTAACTCAGGATAATTGCGCATCAGTTCGGTAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATACGAATCATCCCCTCCGGGCGCGTTTTGAt  <  1:109770/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                    TTTTTGCAGATACTCAGGTGCTGCGCATAATATTCTTTTATTTTTTGTCAAAAGATGCGCAATATAATAATCAGGAATTTCGTCATTAATACGAATATCCAGATCAATATTATCCTGCACTAAATCAATTTGCCGATCGAACAATTCAAAATGCACCTGTAACTCAGGATAATTGCGCATCAGTTCGGTAATAGCTGGCGCAATATGGCTGCGCCCAAAACCAAAACTGCAACCAATACGAATCATCCCCTCCGGGCGCGTTTTGAT  >  NC_000913/3205723‑3205989

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑