Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,703,667	C→A	G61V (GGC→GTC)	dppD ←	dipeptide ABC transporter ATP binding subunit DppD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,703,667	0	C	A	100.0%	124.0 / NA	36	G61V (GGC→GTC)	dppD	dipeptide ABC transporter ATP binding subunit DppD
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (18/18);  total (18/18)

AGCACGGGTTAAGGCTGGTCATCGGGTCCTGGAAGATCATCGCCACTTCGGCACCCACCAGGTTGCGGCGCTCTTTTTCTGAGATACGCTGCAAATCCTGGCCGTTAAACTCCAGTTTTTCTGCCATTACGCGGCCCGGATAATCAATCAGCCCCATAATCGCCAGTGAACTGACCGACTTACCGGAGCCGGACTCACCCACAATCCCGACCACTTCGCCCTGTTTTACGCTGTAGCTGATGCGGTCTACGGCGCGGAACGGCGCGCTTTC  >  NC_000913/3703533‑3703803                                                                                                                                       |                                                                                                                                         aGCACGGGTTAAGGCTGGTCATCGGGTCCTGGAAGATCATCGCCACTTCGGCACCCACCAGGTTGCGGCGCTCTTTTTCTGAGATACGCTGCAAATCCTGGCCGTTAAACTCCAGTTTTTCTGCCATTACGCGGACCGGa                                                                                                                                     >  2:230389/1‑140 (MQ=255)  gCACGGGTTAAGGCTGGTCATCGGGTCCTGGAAGATCATCGCCACTTCGGCACCCACCAGGTTGCGGCGCTCTTTTTCTGAGATACGCTGCAAATCCTGGCCGTTAAACTCCAGTTTTTCTGCCATTACGCGGACCGGAt                                                                                                                                    >  1:20341/1‑140 (MQ=255)        gTTAAGGCTGGTCATCGGGTCCTGGAAGATCATCGCCACTTCGGCACCCACCAGGTTGCGGCGCTCTTTTTCTGAGATACGCTGCAAATCCTGGCCGTTAAACTCCAGTTTTTCTGCCATTACGCGGACCGGATaatcaa                                                                                                                              >  1:445508/1‑140 (MQ=255)        gTTAAGGCTGGTCATCGGGTCCTGGAAGATCATCGCCACTTCGGCACCCACCAGGTTGCGGCGCTCTTTTTCTGAGATACGCTGCAAATCCTGGCCGTTAAACTCCAGTTTTTCTGCCATTACGCGGACCGGATaatcaa                                                                                                                              >  1:769939/1‑140 (MQ=255)        gTTAAGGCTGGTCATCGGGTCCTGGAAGATCATCGCCACTTCGGCACCCACCAGGTTGCGGCGCTCTTTTTCTGAGATACGCTGCAAATCCTGGCCGTTAAACTCCAGTTTTTCTGCCATTACGCGGACCGGATaatcaa                                                                                                                              >  1:62586/1‑140 (MQ=255)         ttAAGGCTGGTCATCGGGTCCTGGAAGATCATCGCCACTTCGGCACCCACCAGGTTGCGGCGCTCTTTTTCTGAGATACGCTGCAAATCCTGGCCGTTAAACTCCAGTTTTTCTGCCATTACGCGGACCGGATaatcaat                                                                                                                             <  1:389067/140‑1 (MQ=255)           aaGGCTGGTCATCGGGTCCTGGAAGATCATCGCCACTTCGGCACCCACCAGGTTGCGGCGCTCTTTTTCTGAGATACGCTGCAAATCCTGGCCGTTAAACTCCAGTTTTTCTGCCATTACGCGGACCGGATAatcaatca                                                                                                                           <  2:445508/140‑1 (MQ=255)              gCTGGTCATCGGGTCCTGGAAGATCATCGCCACTTCGGCACCCACCAGGTTGCGGCGCTCTTTTTCTGAGATACGCTGCAAATCCTGGCCGTTAAACTCCAGTTTTTCTGCCATTACGCGGACCGGATAATCAATCAGcc                                                                                                                        <  1:230389/140‑1 (MQ=255)                   tCATCGGGTCCTGGAAGATCATCGCCACTTCGGCACCCACCAGGTTGCGGCGCTCTTTTTCTGAGATACGCTGCAAATCCTGGCCGTTAAACTCCAGTTTTTCTGCCATTACGCGGACCGGATAATCAATCAGCCCCATa                                                                                                                   <  2:20341/140‑1 (MQ=255)                     aTCGGGTCCTGGAAGATCATCGCCACTTCGGCACCCACCAGGTTGCGGCGCTCTTTTTCTGAGATACGCTGCAAATCCTGGCCGTTAAACTCCAGTTTTTCTGCCATTACGCGGACCGGATAATCAATCAGCCCCATAAt                                                                                                                 <  2:183972/140‑1 (MQ=255)                                                  ggCACCCACCAGGTTGCGGCGCTCTTTTTCTGAGATACGCTGCAAATCCTGGCCGTTAAACTCCAGTTTTTCTGCCATTACGCGGACCGGATAATCAATCAGCCCCATAATCGCCAGTGAACTGACCGACTTAcc                                                                                         <  1:223383/135‑1 (MQ=255)                                                  ggCACCCACCAGGTTGCGGCGCTCTTTTTCTGAGATACGCTGCAAATCCTGGCCGTTAAACTCCAGTTTTTCTGCCATTACGCGGACCGGATAATCAATCAGCCCCATAATCGCCAGTGAACTGACCGACTTAcc                                                                                         >  2:223383/1‑135 (MQ=255)                                                         accaGGTTGCGGCGCTCTTTTTCTGAGATACGCTGCAAATCCTGGCCGTTAAACTCCAGTTTTTCTGCCATTACGCGGACCGGATAATCAATCAGCCCCATAATCGCCAGTGAACTGACCGACTTACCGGAGCCGGACTc                                                                             <  2:769939/140‑1 (MQ=255)                                                                tGCGGCGCTCTTTTTCTGAGATACGCTGCAAATCCTGGCCGTTAAACTCCAGTTTTTCTGCCATTACGCGGACCGGATAATCAATCAGCCCCATAATCGCCAGTGAACTGACCGACTTACCGGAGCCGGACTCACCcaca                                                                      <  2:62586/140‑1 (MQ=255)                                                                 gcggcgCTCTTTTTCTGAGATACGCTGCAAATCCTGGCCGTTAAACTCCAGTTTTTCTGCCATTACGCGGACCGGATAATCAATCAGCCCCATAATCGCCAGTGAACTGACCGACTTACCGGAGCCGGACTCACCCACaa                                                                     >  2:656267/1‑140 (MQ=255)                                                                 gcggcgCTCTTTTTCTGAGATACGCTGCAAATCCTGGCCGTTAAACTCCAGTTTTTCTGCCATTACGCGGACCGGATAATCAATCAGCCCCATAATCGCCAGTGAACTGACCGACTTACCGGAGCCGGACTCACCCACaa                                                                     >  2:491443/1‑140 (MQ=255)                                                                  cggcgCTCTTTTTCTGAGATACGCTGCAAATCCTGGCCGTTAAACTCCAGTTTTTCTGCCATTACGCGGACCGGATAATCAATCAGCCCCATAATCGCCAGTGAACTGACCGACTTACCGGAGCCGGACTc                                                                             <  1:138453/131‑1 (MQ=255)                                                                  cggcgCTCTTTTTCTGAGATACGCTGCAAATCCTGGCCGTTAAACTCCAGTTTTTCTGCCATTACGCGGACCGGATAATCAATCAGCCCCATAATCGCCAGTGAACTGACCGACTTACCGGAGCCGGACTc                                                                             >  2:138453/1‑131 (MQ=255)                                                                  cggcgCTCTTTTTCTGAGATACGCTGCAAATCCTGGCCGTTAAACTCCAGTTTTTCTGCCATTACGCGGACCGGATAATCAATCAGCCCCATAATCGCCAGTGAACTGACCGACTTACCGGAGCCGGACTCACCCACAAt                                                                    >  1:391244/1‑140 (MQ=255)                                                                  cggcgCTCTTTTTCTGAGATACGCTGCAAATCCTGGCCGTTAAACTCCAGTTTTTCTGCCATTACGCGGACCGGATAATCAATCAGCCCCATAATCGCCAGTGAACTGACCGACTTACCGGAGCCGGACTCACCCACAAt                                                                    >  1:480152/1‑140 (MQ=255)                                                                     cgcTCTTTTTCTGAGATACGCTGCAAATCCTGGCCGTTAAACTCCAGTTTTTCTGCCATTACGCGGACCGGATAATCAATCAGCCCCATAATCGCCAGTGAACTGACCGACTTACCGGAGCCGGACTCACCCACAATccc                                                                 <  2:704542/140‑1 (MQ=255)                                                                       ctctTTTTCTGAGATACGCTGCAAATCCTGGCCGTTAAACTCCAGTTTTTCTGCCATTACGCGGACCGGATAATCAATCAGCCCCATAATCGCCAGTGAACTGACCGACTTACCGGAGCCGGACTCACCCACAATCCCGa                                                               <  1:491443/140‑1 (MQ=255)                                                                                 gagaTACGCTGCAAATCCTGGCCGTTAAACTCCAGTTTTTCTGCCATTACGCGGACCGGATAATCAATCAGCCCCATAATCGCCAGTGAACTGACCGACTTACCGGAGCCGGACTCACCCACAATCCCGACCACTTCGcc                                                     <  2:460098/140‑1 (MQ=255)                                                                                    aTACGCTGCAAATCCTGGCCGTTAAACTCCAGTTTTTCTGCCATTACGCGGACCGGATAATCAATCAGCCCCATAATCGCCAGTGAACTGACCGACTTACCGGAGCCGGACTCACCCACAATCCCGACCACTTCGCCCTg                                                  >  2:142538/1‑140 (MQ=255)                                                                                       cGCTGCAAATCCTGGCCGTTAAACTCCAGTTTTTCTGCCATTACGCGGACCGGATAATCAATCAGCCCCATAATCGCCAGTGAACTGACCGACTTACCGGAGCCGGACTCACCCACAATCCCGACCACTTCGCCCTGtt                                                <  1:734400/139‑1 (MQ=255)                                                                                       cGCTGCAAATCCTGGCCGTTAAACTCCAGTTTTTCTGCCATTACGCGGACCGGATAATCAATCAGCCCCATAATCGCCAGTGAACTGACCGACTTACCGGAGCCGGACTCACCCACAATCCCGACCACTTCGCCCTGtt                                                >  2:734400/1‑139 (MQ=255)                                                                                          tGCAAATCCTGGCCGTTAAACTCCAGTTTTTCTGCCATTACGCGGACCGGATAATCAATCAGCCCCATAATCGCCAGTGAACTGACCGACTTACCGGAGCCGGACTCACCCACAATCCCGACCACTTCGCCCTGTTTTAc                                            >  2:423281/1‑140 (MQ=255)                                                                                            cAAATCCTGGCCGTTAAACTCCAGTTTTTCTGCCATTACGCGGACCGGATAATCAATCAGCCCCATAATCGCCAGTGAACTGACCGACTTACCGGAGCCGGACTCACCCACAATCCCGACCACTTCGCCCTGTTTTACGc                                          <  2:561019/140‑1 (MQ=255)                                                                                              aaTCCTGGCCGTTAAACTCCAGTTTTTCTGCCATTACGCGGACCGGATAATCAATCAGCCCCATAATCGCCAGTGAACTGACCGACTTACCGGAGCCGGACTCACCCACAATCCCGACCACTTCGCCCTGTTTTACGCTg                                        >  2:161768/1‑140 (MQ=255)                                                                                                    ggCCGTTAAACTCCAGTTTTTCTGCCATTACGCGGACCGGATAATCAATCAGCCCCATAATCGCCAGTGAACTGACCGACTTACCGGAGCCGGACTCACCCACAATCCCGACCACTTCGCCCTGTTTTACGCTGTAGCTg                                  >  1:270336/1‑140 (MQ=255)                                                                                                      ccGTTAAACTCCAGTTTTTCTGCCATTACGCGGACCGGATAATCAATCAGCCCCATAATCGCCAGTGAACTGACCGACTTACCGGAGCCGGACTCACCCACAATCCCGACCACTTCGCCCTGTTTTACGCTGTAGCTGAt                                <  1:502405/140‑1 (MQ=255)                                                                                                       cGTTAAACTCCAGTTTTTCTGCCATTACGCGGACCGGATAATCAATCAGCCCCATAATCGCCAGTGAACTGACCGACTTACCGGAGCCGGACTCACCCACAATCCCGACCACTTCGCCCTGTTTTACGCTGTAGCTGATg                               <  1:54928/140‑1 (MQ=255)                                                                                                           aaaCTCCAGTTTTTCTGCCATTACGCGGACCGGATAATCAATCAGCCCCATAATCGCCAGTGAACTGACCGACTTACCGGAGCCGGACTCACCCACAATCCCGACCACTTCGCCCTGTTTTACGCTGTAGCTGATGCGGt                           >  2:436051/1‑140 (MQ=255)                                                                                                              cTCCAGTTTTTCTGCCATTACGCGGACCGGATAATCAATCAGCCCCATAATCGCCAGTGAACTGACCGACTTACCGGAGCCGGACTCACCCACAATCCCGACCACTTCGCCCTGTTTTACGCTGTAGCTGATGCGGTCTa                        <  1:656267/140‑1 (MQ=255)                                                                                                                    tttttCTGCCATTACGCGGACCGGATAATCAATCAGCCCCATAATCGCCAGTGAACTGACCGACTTACCGGAGCCGGACTCACCCACAATCCCGACCACTTCGCCCTGTTTTACGCTGTAGCTGATGCGGTCTACGgcgc                  >  1:544599/1‑140 (MQ=255)                                                                                                                                    cgGACCGGATAATCAATCAGCCCCATAATCGCCAGTGAACTGACCGACTTACCGGAGCCGGACTCACCCACAATCCCGACCACTTCGCCCTGTTTTACGCTGTAGCTGATGCGGTCTACGGCGCGGAACGGCGCGCTTtc  <  1:423281/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                         AGCACGGGTTAAGGCTGGTCATCGGGTCCTGGAAGATCATCGCCACTTCGGCACCCACCAGGTTGCGGCGCTCTTTTTCTGAGATACGCTGCAAATCCTGGCCGTTAAACTCCAGTTTTTCTGCCATTACGCGGCCCGGATAATCAATCAGCCCCATAATCGCCAGTGAACTGACCGACTTACCGGAGCCGGACTCACCCACAATCCCGACCACTTCGCCCTGTTTTACGCTGTAGCTGATGCGGTCTACGGCGCGGAACGGCGCGCTTTC  >  NC_000913/3703533‑3703803

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑