Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,101,215	C→A	R157I (AGA→ATA)	csgG ←	curli secretion channel

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,101,215	0	C	A	100.0%	161.6 / NA	47	R157I (AGA→ATA)	csgG	curli secretion channel
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (22/25);  total (22/25)

TATCGTCTTACTGGTGTTCACCGAAGAAAGGATCTCGCCGGTACTCACATTGACGACGCGCAGGTTCACGGCAATCTGATCGAGCTGGTATTGCGTGTCGG‑CACCGATGCCAAAATATCTTGCCCCAACCCCGCCAGATTTGACGTTGCTTTCATAACCGATAATCGAACCTTCAACCATGATATTTGCCGCCGTTAAAGATTGCAGCGGGATTCGGTTATTAATGGCAACCGTGCCGTTTTCTTGTGCCGC  >  NC_000913/1101097‑1101348                                                                                                                        |                                                                                                                                      tATCGTCTTACTGGTGTTCACCGAAGAAAGGATCTCGCCGGTACTCACATTGACGACGCGCAGGTTCACGGCAATCTGATCGAGCTGGTATTGCGTGTCGG‑CACCGATGCCAAAATATATTGCCCCAACCCCGCCAGAtt                                                                                                                  >  2:795777/1‑140 (MQ=255) tATCGTCTTACTGGTGTTCACCGAAGAAAGGATCTCGCCGGTACTCACATTGACGACGCGCAGGTTCACGGCAATCTGATCGAGCTGGTATTGCGTGTCGG‑CACCGATGCCAAAATATATTGCCCCAACCCCGCCAGAtt                                                                                                                  >  1:399690/1‑140 (MQ=255) tATCGTCTTACTGGTGTTCACCGAAGAAAGGATCTCGCCGGTACTCACATTGACGACGCGCAGGTTCACGGCAATCTGATCGAGCTGGTATTGCGTGTCGG‑CACCGATGCCAAAATATATTGCCCCAACCCCGCCAGAtt                                                                                                                  >  1:423303/1‑140 (MQ=255)        ttACTGGTGTTCACCGAAGAAAGGATCTCGCCGGTACTCACATTGACGACGCGCAGGTTCACGGCAATCTGATCGAGCTGGTATTGCGTGTCGG‑CACCGATGCCAAAATATATTGCCCCAACCCCGCCAGATTTGa                                                                                                               <  1:196938/136‑1 (MQ=255)        ttACTGGTGTTCACCGAAGAAAGGATCTCGCCGGTACTCACATTGACGACGCGCAGGTTCACGGCAATCTGATCGAGCTGGTATTGCGTGTCGG‑CACCGATGCCAAAATATATTGCCCCAACCCCGCCAGATTTGa                                                                                                               >  2:196938/1‑136 (MQ=255)           cTGGTGTTCTCCGAAGAAAGGATCTCGCCGGTACTCACATTGACGACGCGCAGGTTCACGGCAATCTGATCGAGCTGGTATTGCGTGTCGG‑CACCGATGCCAAAATATATTGCCCCAACCCCGCCAGATTTg                                                                                                                <  2:70559/132‑1 (MQ=255)           cTGGTGTTCTCCGAAGAAAGGATCTCGCCGGTACTCACATTGACGACGCGCAGGTTCACGGCAATCTGATCGAGCTGGTATTGCGTGTCGG‑CACCGATGCCAAAATATATTGCCCCAACCCCGCCAGATTTg                                                                                                                >  1:70559/1‑132 (MQ=255)               tgtTCACCGAAGAAAGGATCTCGCCGGTACTCACATTGACGACGCGCAGGTTCACGGCAATCTGATCGAGCTGGTATTGCGTGTCGG‑CACCGATGCTAAAATATATTGCCCCAACCCCGCCAGATTTGACGTTGCTTTCa                                                                                                    <  1:687728/140‑1 (MQ=255)                gtTCACCGAAGAAAGGATCTCGCCGGTACTCACATTGACGACGCGCAGGTTCACGGCAATCTGATCGAGCTGGTATTGCGTGTCGG‑CACCGATGCCAAAATATATTGCCCCAACCCCGCCAGATTTGACGTTGCTTTCAt                                                                                                   <  1:706241/140‑1 (MQ=255)                gtTCACCGAAGAAAGGATCTCGCCGGTACTCACATTGACGACGCGCAGGTTCACGGCAATCTGATCGAGCTGGTATTGCGTGTCGG‑CACCGATGCCAAAATATATTGCCCCAACCCCGCCAGATTTGACGTTGCTTTCAt                                                                                                   >  1:522053/1‑140 (MQ=255)                 ttCACCGAAGAAAGGATCTCGCCGGTACTCACATTGACGACGCGCAGGTTCACGGCAATCTGATCGAGCTGGTATTGCGTGTCGG‑CACCGATGCCAAAATATATTGCCCCAACCCCGCCAGATTTGACGTTGCt                                                                                                        >  1:662041/1‑134 (MQ=255)                 ttCACCGAAGAAAGGATCTCGCCGGTACTCACATTGACGACGCGCAGGTTCACGGCAATCTGATCGAGCTGGTATTGCGTGTCGG‑CACCGATGCCAAAATATATTGCCCCAACCCCGCCAGATTTGACGTTGCt                                                                                                        <  2:662041/134‑1 (MQ=255)                 ttCACCGAAGAAAGGATCTCGCCGGTACTCACATTGACGACGCGCAGGTTCACGGCAATCTGATCGAGCTGGTATTGCGTGTCGG‑CACCGATGCCAAAATATATTGCCCCAACCCCGCCAGATTTGACGTTGCTTTCATa                                                                                                  >  1:51335/1‑140 (MQ=255)                           aaaGGATCTCGCCGGTACTCACATTGACGACGCGCAGGTTCACGGCAATCTGATCGAGCTGGTATTGCGTGTCGG‑CACCGATGCCAAAATATATTGCCCCAACCCCGCCAGATTTGACGTTGCTTTCATAACCGATAATc                                                                                        <  2:423303/140‑1 (MQ=255)                            aaGGATCTCGCCGGTACTCACATTGACGACGCGCAGGTTCACGGCAATCTGATCGAGCTGGTATTGCGTGTCGG‑CACCGATGCCAAAATATATTGCCCCAACCCCGCCAGATTTGACGTTGCTTTCATAACCGATAATCg                                                                                       <  2:53445/140‑1 (MQ=255)                             aGGATCTCGCCGGTACTCACATTGACGACGCGCAGGTTCACGGCAATCTGATCGAGCTGGTATTGCGTGTCGG‑CACCGATGCCAAAATATATTGCCCCAACCCCGCCAGATTTGACGTTGCTTTCATAACCGATAATCGa                                                                                      <  2:51335/140‑1 (MQ=255)                                 tctcGCCGGTACTCACATTGACGACGCGCAGGTTCACGGCAATCTGATCGAGCTGGTATTGCGTGTCGG‑CACCGATGCCAAAATATATTGCCCCAACCCCGCCAGATTTGACGTTGCTTTCATAACCGATAATCgctgtc                                                                                  <  2:399690/140‑6 (MQ=255)                                  ctcGCCGGTACTCACATTGACGACGCGCAGGTTCACGGCAATCTGATCGAGCTGGTATTGCGTGTCGG‑CACCGATGCCAAAATATATTGCCCCAACCCCGCCAGATTTGACGTTGCTTTCATAACCGATAATCGAACCtt                                                                                 >  2:12959/1‑140 (MQ=255)                                  ctcGCCGGTACTCACATTGACGACGCGCAGGTTCACGGCAATCTGATCGAGCTGGTATTGCGTGTCGG‑CACCGATGCCAAAATATATTGCCCCAACCCCGCCAGATTTGACGTTGCTTTCATAACCGATAATCGAACCtt                                                                                 <  1:549675/140‑1 (MQ=255)                                   tcGCCGGTACTCACATTGACGACGCGCAGGTTCACGGCAATCTGATCGAGCTGGTATTGCGTGTCGG‑CACCGATGCCAAAATATATTGCCCCAACCCCGCCAGATTTGACGTTGCTTTCATAACCGATAATCGAACCTTc                                                                                <  1:795777/140‑1 (MQ=255)                                   tcGCCGGTACTCACATTGACGACGCGCAGGTTCACGGCAATCTGATCGAGCTGGTATTGCGTGTCGG‑CACCGATGCCAAAATATATTGCCCCAACCCCGCCAGATTTGACGTTGCTTTCATAACCGATAATCGAACCTTc                                                                                <  2:125891/140‑1 (MQ=255)                                   tcGCCGGTACTCACATTGACGACGCGCAGGTTCACGGCAATCTGATCGAGCTGGTATTGCGTGTCGG‑CACCGATGCCAAAATATATTGCCCCAACCCCGCCAGATTTGACGTTGCTTTCATAACCGATAATCGAACCTTc                                                                                <  1:4395/140‑1 (MQ=255)                                   tcGCCGGTACTCACATTGACGACGCGCAGGTTCACGGCAATCTGATCGAGCTGGTATTGCGTGTCGG‑CACCGATGCCAAAATATATTGCCCCAACCCCGCCAGATTTGACGTTGCTTTCATAACCGATAATCGAACCTTc                                                                                <  1:12959/140‑1 (MQ=255)                                          tACTCACATTGACGACGCGCAGGTTCACGGCAATCTGATCGAGCTGGTATTGCGTGTCGG‑CACCGATGCCAAAATATATTGCCCCAACCCCGCCAGATTTGACGTTGCTTTCATAACCGATAATCGAACCTTc                                                                                <  1:548305/133‑1 (MQ=255)                                          tACTCACATTGACGACGCGCAGGTTCACGGCAATCTGATCGAGCTGGTATTGCGTGTCGG‑CACCGATGCCAAAATATATTGCCCCAACCCCGCCAGATTTGACGTTGCTTTCATAACCGATAATCGAACCTTc                                                                                >  2:548305/1‑133 (MQ=255)                                                    gacgacGCGCAGGTTCACGGCAATCTGATCGAGCTGGTATTGCGTGTCGG‑CACCGATGCCAAAATATATTGCCCCAACCCCGCCAGATTTGACGTTGCTTTCATAACCGATAATCGAACCTTCAACCATGATATTTgccg                                                               <  2:522053/140‑1 (MQ=255)                                                    gacgacGCGCAGGTTCACGGCAATCTGATCGAGCTGGTATTGCGTGTCGG‑CACCGATGCCAAAATATATTGCCCCAACCCCGCCAGATTTGACGTTGCTTTCATAACCGATAATCGAACCTTCAACCATGATATTTgc                                                                 <  2:507505/138‑1 (MQ=255)                                                    gacgacGCGCAGGTTCACGGCAATCTGATCGAGCTGGTATTGCGTGTCGG‑CACCGATGCCAAAATATATTGCCCCAACCCCGCCAGATTTGACGTTGCTTTCATAACCGATAATCGAACCTTCAACCATGATATTTgc                                                                 >  1:507505/1‑138 (MQ=255)                                                      cgacgCGCAGGTTCACGGCAATCTGATCGAGCTGGTATTGCGTGTCGG‑CACCGATGCCAAAATATATTGCCCCAACCCCGCCAGATTTGACGTTGCTTTCATAACCGATAATCGAACCTTCAACCATGATATTTgccgcc                                                             >  2:441786/1‑140 (MQ=255)                                                           cgcAGGTTCACGGCAATCTGATCGAGCTGGTATTGCGTGTCGG‑CACCGATGCCAAAATATATTGCCCCAACCCCGCCAGATTTGACGTTGCTTTCATAACCGATAATCGAACCTTCAACCATGATGTTTGCCGCCGTTaa                                                        >  1:789607/1‑140 (MQ=255)                                                           cgcAGGTTCACGGCAATCTGATCGAGCTGGTATTGCGTGTCGG‑CACCGATGCCAAAATATATTGCCCCAACCCCGCCAGATTTGACGTTGCTTTCATAACCGATAATCGAACCTTCAACCATGATATTTGCCGCCGTTaa                                                        >  1:276107/1‑140 (MQ=255)                                                                           tCTGATCGAGCTGGTATTGCGTGTCGG‑CACCGATGCCAAAATATATTGCCCCAACCCCGCCAGATTTGACGTTGCTTTCATAACCGATAATCGAACCTTCAACCATGATATTTGCCGCCGTTAAAGATTGCAGCGGGAtt                                        <  1:195002/140‑1 (MQ=255)                                                                                 cGAGGTGGATTTGCGT‑TCGGCCACCAATGCCAAAATATATCGCCCCACCCCCGCCAGATTTGACGTTGCTTTCATAACCGATAATCGATCCTTCAACCATGATATTTGCCGCCGTTAAAGATTGCAGCGGGATTCGGtta                                  <  2:308046/140‑1 (MQ=255)                                                                                     cTGGTATTGCGTGTCGG‑CACCGATGCCAAAATATATTGCCCCAACCCCGCCAGATTTGACGTTGCTTTCATAACCGATAATCGAACCTTCAACCATGATATTTGCCGCCGTTAAAGATTGCAGCGGGATTCGGttatt                                <  1:231960/138‑1 (MQ=255)                                                                                     cTGGTATTGCGTGTCGG‑CACCGATGCCAAAATATATTGCCCCAACCCCGCCAGATTTGACGTTGCTTTCATAACCGATAATCGAACCTTCAACCATGATATTTGCCGCCGTTAAAGATTGCAGCGGGATTCGGttatt                                >  2:231960/1‑138 (MQ=255)                                                                                       ggTATTGCGTGTCGG‑CACCGATGCCAAAATATATTGCCCCAACCCCGCCAGATTTGACGTTGCTTTCATAACCGATAATCGAACCTTCAACCATGATATTTGCCGCCGTTAAAGATTGCAGCGGGATTCGGTTATTAATg                            >  1:326734/1‑140 (MQ=255)                                                                                                tgtCGG‑CACCGATGCCAAAATATATTGCCCCAACCCCGCCAGATTTGACGTTGCTTTCATAACCGATAATCGAACCTTCAACCATGATATTTGCCGCCGTTAAAGATTGCAGCGGGATTCGGTTATTAAt                             <  1:622220/130‑1 (MQ=255)                                                                                                tgtCGG‑CACCGATGCCAAAATATATTGCCCCAACCCCGCCAGATTTGACGTTGCTTTCATAACCGATAATCGAACCTTCAACCATGATATTTGCCGCCGTTAAAGATTGCAGCGGGATTCGGTTATTAAt                             >  2:622220/1‑130 (MQ=255)                                                                                                  tCGG‑CACCGATGCCAAAATATATTGCCCCAACCCCGCCAGATTTGACGTTGCTTTCATAACCGATAATCGAACCTTCAACCATGATATTTGCCGCCGTTAAAGATTGCAGCGGGATTCGGtta                                  >  1:565324/1‑123 (MQ=255)                                                                                                  tCGG‑CACCGATGCCAAAATATATTGCCCCAACCCCGCCAGATTTGACGTTGCTTTCATAACCGATAATCGAACCTTCAACCATGATATTTGCCGCCGTTAAAGATTGCAGCGGGATTCGGtta                                  <  2:565324/123‑1 (MQ=255)                                                                                                  tCGG‑CACCGATGCCAAAATATATTGCCCCAACCCCGCCAGATTTGACGTTGCTTTCATAACCGATAATCGAACCTTCAACCATGATATTTGCCGCCGTTAAAGATTGCAGCGGGATTCGGTTATTAATGGCAACCGTGcc                 >  1:786784/1‑140 (MQ=255)                                                                                                  tCGG‑CACCGATGCCAAAATATATTGCCCCAACCCCGCCAGATTTGACGTTGCTTTCATAACCGATAATCGAACCTTCAACCATGATATTTGCCGCCGTTAAAGATTGCAGCGGGATTCGGTTATTAATGGCAACCGTGcc                 >  1:308039/1‑140 (MQ=255)                                                                                                  tCGG‑CACCGATGCCAAAATATATTGCCCCAACCCCGCCAGATTTGACGTTGCTTTCATAACCGATAATCGAACCTTCAACCATGATATTTGCCGCCGTTAAAGATTGCAGCGGGATTCGGTTATTAATGGCAACCGTGcc                 >  2:667214/1‑140 (MQ=255)                                                                                                   cGG‑CACCGATGCCAAAATATATTGCCCCAACCCCGCCAGATTTGACGTTGCTTTCATAACCGATAATCGAACCTTCAACCATGATATTTGCCGCCGTTAAAGATTGCAGCGGGATTCGGTTATTAATGGCAACCGTGCCg                <  1:667214/140‑1 (MQ=255)                                                                                                     g‑CACCGATGCCAAAATATATTGCCCCAACCCCGCCAGATTTGACGTTGCTTTCATAACCGATAATCGAACCTTCAACCATGATATTTGCCGCCGTTAAAGATTGCAGCGGGATTCGGTTATTAATGGCAACCGTGCCGtt              <  2:308039/140‑1 (MQ=255)                                                                                                             tGCCAAAATATATTGCCCCAACCCCGCCAGATTTGACGTTGCTTTCATAACCGATAATCGAACCTTCAACCATGATATTTGCCGCCGTTAAAGATTGCAGCGGGATTCGGTTATTAATGGCAACCGTg                   <  2:272917/128‑1 (MQ=255)                                                                                                             tGCCAAAATATATTGCCCCAACCCCGCCAGATTTGACGTTGCTTTCATAACCGATAATCGAACCTTCAACCATGATATTTGCCGCCGTTAAAGATTGCAGCGGGATTCGGTTATTAATGGCAACCGTg                   >  1:272917/1‑128 (MQ=255)                                                                                                                cAAAATATATTGCCCCAACCCCGCCAGATTTGACGTTGCTTTCATAACCGATAATCGAACCTTCAACCATGATATTTGCCGCCGTTAAAGATTGCAGCGGGATTCGGTTATTAATGGCAACCGTGCCATTTTCTTGTGcc    <  1:487948/140‑1 (MQ=255)                                                                                                                  aaaTATATTGCCCCAACCCCGCCAGATTTGACGTTGCTTTCATAACCGATAATCGAACCTTCAACCATGATATTTGCCGCCGTTAAAGATTGCAGCGGGATTCGGTTATTAATGGCAACCGTGCCGTTTTCTTGTGCCGc  <  2:786784/140‑1 (MQ=255)                                                                                                                        |                                                                                                                                      TATCGTCTTACTGGTGTTCACCGAAGAAAGGATCTCGCCGGTACTCACATTGACGACGCGCAGGTTCACGGCAATCTGATCGAGCTGGTATTGCGTGTCGG‑CACCGATGCCAAAATATCTTGCCCCAACCCCGCCAGATTTGACGTTGCTTTCATAACCGATAATCGAACCTTCAACCATGATATTTGCCGCCGTTAAAGATTGCAGCGGGATTCGGTTATTAATGGCAACCGTGCCGTTTTCTTGTGCCGC  >  NC_000913/1101097‑1101348

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑