Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,212,250	C→A	G238V (GGC→GTC)	btsR ←	DNA‑binding transcriptional activator BtsR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,212,250	0	C	A	100.0%	144.7 / NA	42	G238V (GGC→GTC)	btsR	DNA‑binding transcriptional activator BtsR
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (22/20);  total (22/20)

CCCAGCGCCAGAATACGCACCAGGTCATTATTATTGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATATCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCATTTTAGCAGTCTTTTACAGGCCAATCGCCTCTTTTAAGCTTTTCAGATAACGGCGGCTGACCGGCACGGTTAAGCCATTACGCAAAATCAACTCGGCCTGGCCGTTATCTTCCAGACGAATCTCCTGTAAATGCGCGAGGTTAACCAGATACTGAC  >  NC_000913/2212114‑2212385                                                                                                                                         |                                                                                                                                        cccAGCGCCAGAATACGCACCAGGTCATTATTATTGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATATCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCATTTTAGCAGTCTTTTACAGGACaa                                                                                                                                      >  1:233992/1‑140 (MQ=255)              tACGCACCAGGTCATTATTATTGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATATCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCATTTTAGCAGTCTTTTACAGGACAATCGCCTCTTTTaa                                                                                                                         <  2:40406/140‑1 (MQ=255)                   aCCAGGTCATTATTATTGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATATCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCATTTTAGCAGTCTTTTACAGGACAATCGCCTCTTTTAAGCttt                                                                                                                    >  1:213155/1‑140 (MQ=255)                        gTCATTATTATTGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATATCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCATTTTAGCAGTCTTTTACAGGACAATCGCCTCTTTTAAGCTTTTCAGa                                                                                                               >  2:246249/1‑140 (MQ=255)                        gTCATTATTATTGGCCTTCAAAATGTAGCGCACGCTGCGCAGAATATCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCATTTTAGCAGTCTTTTACAGGACAATCGCCTCTTTTAAGCTTTTCAGa                                                                                                               >  2:457253/1‑140 (MQ=255)                               ttattGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATATCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCATTTTAGCAGTCTTTTACAGGACAATCGCCTCTTTTAAGCTTTTCAGATAAcggc                                                                                                        >  1:199789/1‑140 (MQ=255)                                  ttGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATATCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCATTTTAGCAGTCTTTTACAGGACAATCGCCTCTTTTAAGCTTTTCAGATAACggcggc                                                                                                     >  1:28182/1‑140 (MQ=255)                                  ttGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATATCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCATTTTAGCAGTCTTTTACAGGACAATCGCCTCTTTTAAGCTTTTCAGATAACggcggc                                                                                                     >  2:626502/1‑140 (MQ=255)                                     gCTTTCAAAATGTAGCGCACGCTGCGCAGAATATCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCATTTTAGCAGTCTTTTACAGGACAATCGCCTCTTTTAAGCTTTTCAGATAACGGCGGCTGa                                                                                                  <  2:199789/140‑1 (MQ=255)                                       tttCAAAATGTAGCGCACGCTGCGCAGAATATCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCATTTTAGCAGTCTTTTACAGGACAATCGCCTCTTTTAAGCTTTTCAGATAACGGCGGCTGAcc                                                                                                >  2:796356/1‑140 (MQ=255)                                       tttCAAAATGTAGCGCACGCTGCGCAGAATATCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCATTTTAGCAGTCTTTTACAGGACAATCGCCTCTTTTAAGCTTTTCAGATAACGGCGGCTGAcc                                                                                                >  2:679647/1‑140 (MQ=255)                                         tCAAAATGTAGCGCACGCTGCGCAGAATATCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCATTTTAGCAGTCTTTTACAGGACAATCGCCTCTTTTAAGCTTTTCAGATAACGGCGGCTGACCgg                                                                                              >  2:291490/1‑140 (MQ=255)                                          cAAAATGTAGCGCACGCTGCGCAGAATATCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCATTTTAGCAGTCTTTTACAGGACAATCGCCTCTTTTAAGCTTTTCAGATAACGGCGGCTGACCgg                                                                                              >  1:604467/1‑139 (MQ=255)                                          cAAAATGTAGCGCACGCTGCGCAGAATATCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCATTTTAGCAGTCTTTTACAGGACAATCGCCTCTTTTAAGCTTTTCAGATAACGGCGGCTGACCgg                                                                                              <  2:604467/139‑1 (MQ=255)                                          cAAAATGTAGCGCACGCTGCGCAGAATATCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCATTTTAGCAGTCTTTTACAGGACAATCGCCTCTTTTAAGCTTTTCAGATAACGGCGGCTGACCGGc                                                                                             <  2:28182/140‑1 (MQ=255)                                           aaaaTGTAGCGCACGCTGCGCAGAATATCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCATTTTAGCAGTCTTTTACAGGACAATCGCCTCTTTTAAGCTTTTCAGATAACGGCGGCTGACCGGCa                                                                                            <  1:796356/140‑1 (MQ=255)                                            aaaTGTAGCGCACGCTGCGCAGAATATCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCATTTTAGCAGTCTTTTACAGGACAATCGCCTCTTTTAAGCTTTTCAGATAACGGCGGCTGACCGGCAc                                                                                           <  1:679647/140‑1 (MQ=255)                                            aaaTGTAGCGCACGCTGCGCAGAATATCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCATTTTAGCAGTCTTTTACAGGACAATCGCCTCTTTTAAGCTTTTCAGATAACGGCGGCTGACCGGCAc                                                                                           <  1:434291/140‑1 (MQ=255)                                                    cgcACGCTGCGCAGAATATCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCATTTTAGCAGTCTTTTACAGGACAATCGCCTCTTTTAAGCTTTTCAGATAACGGCGGCTGACCGGCACGGTTAAGc                                                                                   >  1:29308/1‑140 (MQ=255)                                                    cgcACGCTGCGCAGAATATCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCATTTTAGCAGTCTTTTACAGGACAATCGCCTCTTTTAAGCTTTTCAGATAACGGCGGCTGACCGGCACGGTTAAGc                                                                                   >  1:512656/1‑140 (MQ=255)                                                          cTGCGCAGAATATCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCATTTTAGCAGTCTTTTACAGGACAATCGCCTCTTTTAAGCTTTTCAGATAACGGCGGCTGACCGGCACGGTTAAGCCATTAc                                                                             <  1:626502/140‑1 (MQ=255)                                                                    tatCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCATTTTAGCAGTCTTTTACAGGACAATCGCCTCTTTTAAGCTTTTCAGATAACGGCGGCTGACCGGCACGGTTAAGCCATTACGCAAAATCaa                                                                   <  1:499062/140‑1 (MQ=255)                                                                       cGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCATTTTAGCAGTCTTTTACAGGACAATCGCCTCTTTTAAGCTTTTCAGATAACGGCGGCTGACCGGCACGGTTAAGCCATTACGCAAAATCAACTc                                                                >  2:697171/1‑140 (MQ=255)                                                                          tACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCATTTTAGCAGTCTTTTACAGGACAATCGCCTCTTTTAAGCTTTTCAGATAACGGCGGCTGACCGGCACGGTTAAGCCATTACGCAAAATCAACTCGGc                                                             <  1:457253/140‑1 (MQ=255)                                                                          tACTTAGCATGAGGCCTTCAGATGTTGATGAGGCAAAAAGCCATTTTAGCAGTCTTTTACAGGACAATCGCCTCTTTTAAGCTTTTCAGATAACGGCGGCTGACCGGCACGGTTAAGCCATTACGCAAAATCAACTCGGc                                                             <  1:246249/140‑1 (MQ=255)                                                                            cTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCATTTTAGCAGTCTTTTACAGGACAATCGCCTCTTTTAAGCTTTTCAGATAACGGCGGCTGACCGGCACGGTTAAGCCATTACGCAAAATCAACTCGGCCt                                                           >  2:30524/1‑140 (MQ=255)                                                                            cTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCATTTTAGCAGTCTTTTACAGGACAATCGCCTCTTTTAAGCTTTTCAGATAACGGCGGCTGACCGGCACGGTTAAGCCATTACGCAAAATCAACTCGGCCt                                                           >  1:40541/1‑140 (MQ=255)                                                                               aGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCATTTTAGCAGTCTTTTACAGGACAATCGCCTCTTTTAAGCTTTTCAGATAACGGCGGCTGACCGGCACGGTTAAGCCATTACGCAAAATCAACTCGGCCTGGc                                                        <  2:312177/140‑1 (MQ=255)                                                                                gCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCATTTTAGCAGTCTTTTACAGGACAATCGCCTCTTTTAAGCTTTTCAGATAACGGCGGCTGACCGGCACGGTTAAGCCATTACGCAAAATCAACTCGGCCTGGcc                                                       <  2:40541/140‑1 (MQ=255)                                                                                 cATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCATTTTAGCAGTCTTTTACAGGACAATCGCCTCTTTTAAGCTTTTCAGATAACGGCGGCTGACCGGCACGGTTAAGCCATTACGCAAAATCAACTCGGCCTGGCCg                                                      >  1:570826/1‑140 (MQ=255)                                                                                      ggCCTTCAGGTGTTGATGAGGCAAAAAGCCATTTTAGCAGTCTTTTACAGGACAATCGCCTCTTTTAAGCTTTTCAGATAACGGCGGCTGACCGGCACGGTTAAGCCATTACGCAAAATCAAc                                                                  <  1:634975/123‑1 (MQ=255)                                                                                      ggCCTTCAGGTGTTGATGAGGCAAAAAGCCATTTTAGCAGTCTTTTACAGGACAATCGCCTCTTTTAAGCTTTTCAGATAACGGCGGCTGACCGGCACGGTTAAGCCATTACGCAAAATCAAc                                                                  >  2:634975/1‑123 (MQ=255)                                                                                         cTTCAGGTGTTGATGAGGCAAAAAGCCATTTTAGCAGTCTTTTACAGGACAATCGCCTCTTTTAAGCTTTTCAGATAACGGCGGCTGACCGGCACGGTTAAGCCATTACGCAAAATCAACTCGGCCTGGCCGTTATCTTc                                              >  2:613976/1‑140 (MQ=255)                                                                                           tCAGGTGTTGATGAGGCAAAAAGCCATTTTAGCAGTCTTTTACAGGACAATCGCCTCTTTTAAGCTTTTCAGATAACGGCGGCTGACCGGCACGGTTAAGCCATTACGCAAAATCAACTCGGCCTGGCCGTTATCTTCCa                                            <  2:233992/140‑1 (MQ=255)                                                                                                 gtTGATGAGGCAAAAAGCCATTTTAGCAGTCTTTTACAGGACAATCGCCTCTTTTAAGCTTTTCAGATAACGGCGGCTGACCGGCACGGTTAAGCCATTACGCAAAATCAACTCGGCCTGGCCGTTATCTTCCAGACGaa                                      <  2:512656/140‑1 (MQ=255)                                                                                                 gtTGATGAGGCAAAAAGCCATTTTAGCAGTCTTTTACAGGACAATCGCCTCTTTTAAGCTTTTCAGATAACGGCGGCTGACCGGCACGGTTAAGCCATTACGCAAAATCAACTCGGCCTGGCCGTTATCTTCCAGACGaa                                      <  1:697171/140‑1 (MQ=255)                                                                                                           cAAAAAGCCATTTTAGCAGTCTTTTACAGGACAATCGCCTCTTTTAAGCTTTTCAGATAACGGCGGCTGACCGGCACGGTTAAGCCATTACGCAAAATCAACTCGGCCTGGCCGTTATCTTCCAGACGAATCTCCTGTaa                            <  2:29308/140‑1 (MQ=255)                                                                                                                aGCCATTTTAGCAGTCTTTTACAGGACAATCGCCTCTTTTAAGCTTTTCAGATAACGGCGGCTGACCGGCACGGTTAAGCCATTACGCAAAATCAACTCGGCCTGGCCGTTATCTTCCAGACGAATCTCCTGTAAATgcg                       <  2:268172/140‑1 (MQ=255)                                                                                                                aGCCATTTTAGCAGTCTTTTACAGGACAATCGCCTCTTTTAAGCTTTTCAGATAACGGCGGCTGACCGGCACGGTTAAGCCATTACGCAAAATCAACTCGGCCTGGCCGTTATCTTCCAGACGAATCTCCTGTAAATgcg                       <  1:613976/140‑1 (MQ=255)                                                                                                                    aTTTTAGCAGTCTTTTACAGGACAATCGCCTCTTTTAAGCTTTTCAGATAACGGCGGCTGACCGGCACGGTTAAGCCATTACGCAAAATCAACTCGGCCTGGCCGTTATCTTCCAGACGAATCTCCTGTAAATGCGCGAg                   >  2:5151/1‑140 (MQ=255)                                                                                                                    aTTTTAGCAGTCTTTTACAGGACAATCGCCTCTTTTAAGCTTTTCAGATAACGGCGGCTGACCGGCACGGTTAAGCCATTACGCAAAATCAACTCGGCCTGGCCGTTATCTTCCAGACGAATCTCCTGTAAATGCGCGAg                   >  2:427949/1‑140 (MQ=255)                                                                                                                                     cAGGACAATCGCCTCTTTTAAGCTTTTCAGATAACGGCGGCTGACCGGCACGGTTAAGCCATTACGCAAAATCAACTCGGCCTGGCCGTTATCTTCCAGACGAATCTCCTGTAAATGCGCGAGGTTAACCAGATACTGAc  >  1:769080/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                        CCCAGCGCCAGAATACGCACCAGGTCATTATTATTGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATATCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCATTTTAGCAGTCTTTTACAGGCCAATCGCCTCTTTTAAGCTTTTCAGATAACGGCGGCTGACCGGCACGGTTAAGCCATTACGCAAAATCAACTCGGCCTGGCCGTTATCTTCCAGACGAATCTCCTGTAAATGCGCGAGGTTAACCAGATACTGAC  >  NC_000913/2212114‑2212385

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑