Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	174,372	C→A	D171Y (GAT→TAT)	hemL ←	glutamate‑1‑semialdehyde aminotransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	174,372	0	C	A	100.0%	269.7 / NA	76	D171Y (GAT→TAT)	hemL	glutamate‑1‑semialdehyde aminotransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (42/34);  total (42/34)

AGCGGCGGAACACAGTTCATATTGCCTGCCACCGGCTCGACGATAATACAGGCAATCTCTTGCGGGTATTGCTCAAATGCGGCGCGTACAGAAGCCAGATCATTATAAGTACAGGTTAAGGTATATTTGGCGAAATCTGCCGGAACGCCCGGCGAGTTTGGCTGGCCTAACGTGAGTGCGCCAGAACCGGCTTTCACCAGCAGGCAGTCAGCGTGACCATGGTAACACCCTTCAAATTTAATAATTTTGTCGCGACCGGTAAAACCACGGGC  >  NC_000913/174236‑174507                                                                                                                                         |                                                                                                                                        aGCGGCGGAACACAGTTCATATTGCCTGCCACCGGCTCGACGATAATACAGGCAATCTCTTGCGGGTATTGCTCAAATGCGGCGCGTACAGAAGCCAGATCATTATAAGTACAGGGTAAGGTATATTTGGCGAAATATGc                                                                                                                                      >  2:1226489/1‑140 (MQ=255)   cggcggAACACAGTTCATATTGCCTGCCACCGGCTCGACGATAATACAGGCAATCTCTTGCGGGTATTGCTCAAATGCGGCGCGTACAGAAGCCAGATCATTATAAGTACAGGTTAAGGTATATTTGGCGAAATATGCCg                                                                                                                                    <  1:127168/140‑1 (MQ=255)     gcggAACACAGTTCATATTGCCTGCCACCGGCTCGACGATAATACAGGCAATCTCTTGCGGGTATTGCTCAAATGCGGCGCGTACAGAAGCCAGATCATTATAAGTACAGGTTAAGGTATATTTGGCGAAATATGCCGGa                                                                                                                                  >  2:996618/1‑140 (MQ=255)        gAACACAGTTCATATTGCCTGCCACCGGCTCGACGATAATACAGGCAATCTCTTGCGGGTATTGCTCAAATGCGGCGCGTACAGAAGCCAGATCATTATAAGTACAGGTTAAGGTATATTTGGCGAAATATGCCGGAACg                                                                                                                               <  1:825951/140‑1 (MQ=255)               gTTCATATTGCCTGCCACCGGCTCGACGATAATACAGGCAATCTCTTGCGGGTATTGCTCAAATGCGGCGCGTACAGAAGCCAGATCATTATAAGTACAGGTTAAGGTATATTTGGCGAAATATGCCGGaa                                                                                                                                 <  1:1309085/131‑1 (MQ=255)               gTTCATATTGCCTGCCACCGGCTCGACGATAATACAGGCAATCTCTTGCGGGTATTGCTCAAATGCGGCGCGTACAGAAGCCAGATCATTATAAGTACAGGTTAAGGTATATTTGGCGAAATATGCCGGaa                                                                                                                                 >  2:1309085/1‑131 (MQ=255)                ttCATATTGCCTGCCACCGGCTCGACGATAATACAGGCAATCTCTTGCGGGTATTGCTCAAATGCGGCGCGTACAGAAGCCAGATCATTATAAGTACAGGTTAAGGTATATTTGGCGAAATATGCCGGAACGCCCGGCGa                                                                                                                       >  1:1425472/1‑140 (MQ=255)                      ttGCCTGCCACCGGCTCGACGATAATACAGGCAATCTCTTGCGGGTATTGCTCAAATGCGGCGCGTACAGAAGCCAGATCATTATAAGTACAGGTTAAGGTATATTTGGCGAAATATGCCGGAACGCCCGGCGAGTTtgg                                                                                                                 <  2:1425472/140‑1 (MQ=255)                        gcctgccACCGGCTCGACGATAATACAGGCAATCTCTTGCGGGTATTGCTCAAATGCGGCGCGTACAGAAGCCAGATCATTATAAGTACAGGTTAAGGTATATTTGGCGAAATATGCCGGAACGCCCGGCGAg                                                                                                                      >  2:660024/1‑133 (MQ=255)                        gcctgccACCGGCTCGACGATAATACAGGCAATCTCTTGCGGGTATTGCTCAAATGCGGCGCGTACAGAAGCCAGATCATTATAAGTACAGGTTAAGGTATATTTGGCGAAATATGCCGGAACGCCCGGCGAg                                                                                                                      <  1:660024/133‑1 (MQ=255)                        gcctgccACCGGCTCGACGATAATACAGGCAATCTCTTGCGGGTATTGCTCAAATGCGGCGCGTACAGAAGCCAGATCATTATAAGTACAGGTTAAGGTATATTTGGCGAAATATGCCGGAACGCCCGGCGAGTTtgggt                                                                                                               >  2:368703/1‑138 (MQ=255)                          ctgccACCGGCTCGACGATAATACAGGGAATCTCTTGCGGGTATTGCTCAAATGCGGCGCGTACAGAAGCCAGATCATTATAAGTACAGGTTAAGGTATATTTGGCGAAATATGCCGGAACGCCCGGCGAGTTtggctgg                                                                                                             <  1:1109124/140‑1 (MQ=255)                          ctgccACCGGCTCGACGATAATACAGGCAATCTCTTGCGGGTATTGCTCAAATGCGGCGCGTACAGAAGCCAGATCATTATAAGTACAGGTTAAGGTATATTTGGCGAAATATGCCGGAACGCCCGGCGAGTTtggctgg                                                                                                             <  2:1410488/140‑1 (MQ=255)                          ctgccACCGGCTCGACGATAATACAGGCAATCTCTTGCGGGTATTGCTCAAATGCGGCGCGTACAGAAGCCAGATCATTATAAGTACAGGTTAAGGTATATTTGGCGAAATATGCCGGAACGCCCGGCGAGTTtggctgg                                                                                                             <  1:801242/140‑1 (MQ=255)                           tgccACCGGCTCGACGATAATACAGGCAATCTCTTGCGGGTATTGCTCAAATGCGGCGCGTACAGAAGCCAGATCATTATAAGTACAGGTTAAGGTATATTTGGCGAAATATGCCGGAACGCCCGGCGAGTTtggctggc                                                                                                            <  2:569174/140‑1 (MQ=255)                           tgccACCGGCTCGACGATAATACAGGCAATCTCTTGCGGGTATTGCTCAAATGCGGCGCGTACAGAAGCCAGATCATTATAAGTACAGGTTAAGGTATATTTGGCGAAATATGCCGGAACGCCCGGCGAGTTtggctggc                                                                                                            <  2:737392/140‑1 (MQ=255)                            gccACCGGCTCGACGATAATACAGGCAATCTCTTGCGGGTATTGCTCAAATGCGGCGCGTACAGAAGCCAGATCATTATAAGTACAGGTTAAGGTATATTTGGCGAAATATGCCGGAACGCCCGGCGAGTTTGGCTGGcc                                                                                                           <  1:875830/140‑1 (MQ=255)                             ccACCGGCTCGACGATAATACAGGCAATCTCTTGCGGGTATTGCTCAAATGCGGCGCGTACAGAAGCCAGATCATTATAAGTACAGGTTAAGGTATATTTGGCGAAATATGCCGGAACGCCCGGCGAGTTTGGCTGGCCt                                                                                                          >  2:227777/1‑140 (MQ=255)                             ccACCGGCTCGACGATAATACAGGCAATCTCTTGCGGGTATTGCTCAAATGCGGCGCGTACAGAAGCCAGATCATTATAAGTACAGGTTAAGGTATATTTGGCGAAATATGCCGGAACGCCCGGCGAGTTTGGCTGGCCt                                                                                                          >  1:1109074/1‑140 (MQ=255)                                     tCGACGATAATACAGGCAATCTCTTGCGGGTATTGCTCAAATGCGGCGCGTACAGAAGCCAGATCATTATAAGTACAGGTTAAGGTATATTTGGCGAAATATGCCGGAACGCCCGGCGAGTTTGGCTGGCCTAACGTGAg                                                                                                  <  2:920304/140‑1 (MQ=255)                                        acgaTAATACAGGCAATCTCTTGCGGGTATTGCTCAAATGCGGCGCGTACAGAAGCCAGATCATTATAAGTACAGGTTAAGGTATATTTGGCGAAATATGCCGGAACGCCCGGCGAGTTTGGCTGGCCTAACGTGAGTgc                                                                                               <  1:227777/140‑1 (MQ=255)                                          gaTAATACAGGCAATCTCTTGCGGGTATTGCTCAAATGCGGCGCGTACAGAAGCCAGATCATTATAAGTACAGGTTAAGGTATATTTGGCGAAATATGCCGGAACGCCCGGCGAGTTTGGCTGGCCTAACGTGAGTgcgc                                                                                             <  2:796742/140‑1 (MQ=255)                                                aCAGGCAATCTCTTGCGGGTATTGCTCAAATGCGGCGCGTACAGAAGCCAGATCATTATAAGTACAGGTTAAGGTATATTTGGCGAAATATGCCGGAACGCCCGGCGAGTTTGGCTGGCCTAACGTGAGTGCGCCAGAAc                                                                                       <  1:874629/140‑1 (MQ=255)                                                 cAGGCAATCTCTTGCGGGTATTGCTCAAATGCGGCGCGTACAGAAGCCAGATCATTATAAGTACAGGTTAAGGTATATTTGGCGAAATATGCCGGAACGCCCGGCGAGTTTGGCTGGCCTAACGTGAGTGCGCCAGAAcc                                                                                      >  1:1141382/1‑140 (MQ=255)                                                   ggCAATCTCTTGCGGGTATTGCTCAAATGCGGCGCGTACAGAAGCCAGATCATTATAAGTACAGGTTAAGGTATATTTGGCGAAATATGCCGGAACGCCCGGCGAGTTTGGCTGGCCTAACGTGAGTGCGCCAGAACCgg                                                                                    >  1:129757/1‑140 (MQ=255)                                                    gCAATCTCTTGCGGGTATTGCTCAAATGCGGCGCGTACAGAAGCCAGATCATTATAAGTACAGGTTAAGGTATATTTGGCGAAATATGCCGGAACGCCCGGCGAGTTTGGCTGGCCTAACGTGAGTGCGCCAGAACCGGc                                                                                   >  1:1338984/1‑140 (MQ=255)                                                    gCAATCTCTTGCGGGTATTGCTCAAATGCGGCGCGTACAGAAGCCAGATCATTATAAGTACAGGTTAAGGTATATTTGGCGAAATATGCCGGAACGCCCGGCGAGTTTGGCTGGCCTAACGTGAGTGCGCCAGAACCGGc                                                                                   >  2:523243/1‑140 (MQ=255)                                                      aaTCTCTTGCGGGTATTGCTCAAATGCGGCGCGTACAGAAGCCAGATCATTATAAGTACAGGTTAAGGTATATTTGGCGAAATATGCCGGAACGCCCGGCGAGTTTGGCTGGCCTAACGTGAGTGCGCCAGAACCGGCtt                                                                                 >  1:216887/1‑140 (MQ=255)                                                        tctcTTGCGGGTATTGCTCAAATGCGGCGCGTACAGAAGCCAGATCATTATAAGTACAGGTTAAGGTATATTTGGCGAAATATGCCGGAACGCCCGGCGAGTTTGGCTGGCCTAACGTGAGTGCGCCAGAACCGGCTTTc                                                                               >  2:1424002/1‑140 (MQ=255)                                                         ctctTGCGGGTATTGCTCAAATGCGGCGCGTACAGAAGCCAGATCATTATAAGTACAGGTTAAGGTATATTTGGCGAAATATGCCGGAACGCCCGGCGAGTTTGGCTGGCCTAACGTGAGTGCGCCAGAACCGGCTTTCa                                                                              >  1:1303150/1‑140 (MQ=255)                                                              gCGGGTATTGCTCAAATGCGGCGCGTACAGAAGCCAGATCATTATAAGTACAGGTTAAGGTATATTTGGCGAAATATGCCGGAACGCCCGGCGAGTTTGGCTGGCCTAACGTGAGTGCGCCAGAACCGGCTTTCACcagc                                                                         >  2:1108489/1‑140 (MQ=255)                                                                  gTATTGCTCAAATGCGGCGCGTACAGAAGCCAGATCATTATAAGTACAGGTTAAGGTATATTTGGCGAAATATGCCGGAACGCCCGGCGAGTTTGGCTGGCCTAACGTGAGTGCGCCAGAACCGGCTTTCACCAgcaggc                                                                     >  1:794218/1‑140 (MQ=255)                                                                   tATTGCTCAAATGCGGCGCGTACAGAAGCCAGATCATTATAAGTACAGGTTAAGGTATATTTGGCGAAATATGCCGGAACGCCCGGCGAGTTTGGCTGGCCTAACGTGAGTGCGCCAGAACCGGCTTTCACCAgcaggca                                                                    >  1:733249/1‑140 (MQ=255)                                                                    aTTGCTCAAATGCGGCGCGTACAGAAGCCAGATCATTATAAGTACAGGTTAAGGTATATTTGGCGAAATATGCCGGAACGCCCGGCGAGTTTGGCTGGCCTAACGTGAGTGCGCCAGAACCGGCTTTCACCAgcaggcag                                                                   >  1:1458769/1‑140 (MQ=255)                                                                    aTTGCTCAAATGCGGCGCGTACAGAAGCCAGATCATTATAAGTACAGGTTAAGGTATATTTGGCGAAATATGCCGGAACGCCCGGCGAGTTTGGCTGGCCTAACGTGAGTGCGCCAGAACCGGCTTTCACCAgcaggcag                                                                   >  1:192346/1‑140 (MQ=255)                                                                         tCAAATGCGGCGCGTACAGAAGCCAGATCATTATAAGTACAGGTTAAGGTATATTTGGCGAAATATGCCGGAACGCCCGGCGAGTTTGGCTGGCCTAACGTGAGTGCGCCAGAACCGGCTTTCACCAGCAGGCAGTCAGc                                                              <  2:733249/140‑1 (MQ=255)                                                                           aaaTGCGGCGCGTACAGAAGCCAGATCATTATAAGTACAGGTTAAGGTATATTTGGCGAAATATGCCGGAACGCCCGGCGAGTTTGGCTGGCCTAACGTGAGTGCGCCAGAACCGGCTTTCACCAGCAGGCAGTCAGCGt                                                            <  2:1303150/140‑1 (MQ=255)                                                                              tGCGGCGCGTACAGAAGCCAGATCATTATAAGTACAGGTTAAGGTATATTTGGCGAAATATGCCGGAACGCCCGGCGAGTTTGGCTGGCCTAACGTGAGTGCGCCAGAACCGGCTTTCGCCAGCAGGCAGTCAGCGTGAc                                                         <  2:129757/140‑1 (MQ=255)                                                                              tGCGGCGCGTACAGAAGCCAGATCATTATAAGTACAGGTTAAGGTATATTTGGCGAAATATGCCGGAACGCCCGGCGAGTTTGGCTGGCCTAACGTGAGTGCGCCAGAACCGGCTTTCACCAGCAGGCAGTCAGCGTGAc                                                         <  2:1458769/140‑1 (MQ=255)                                                                              tGCGGCGCGTACAGAAGCCAGATCATTATAAGTACAGGTTAAGGTATATTTGGCGAAATATGCCGGAACGCCCGGCGAGTTTGGCTGGCCTAACGTGAGTGCGCCAGAACCGGCTTTCACCAGCAGGCAGTCAGCGTGAc                                                         <  2:1060269/140‑1 (MQ=255)                                                                              tGCGGCGCGTACAGAAGCCAGATCATTATAAGTACAGGTTAAGGTATATTTGGCGAAATATGCCGGAACGCCCGGCGAGTTTGGCTGGCCTAACGTGAGTGCGCCAGAACCGGCTTTCACCAGCAGGCAGTCAGCGTGAc                                                         <  1:1108489/140‑1 (MQ=255)                                                                              tGCGGCGCGTACAGAAGCCAGATCATTATAAGTACAGGTTAAGGTATATTTGGCGAAATATGCCGGAACGCCCGGCGAGTTTGGCTGGCCTAACGTGAGTGCGCCAGAACCGGCTTTCACCAGCAGGCAGTCAGCGTGAc                                                         <  2:676408/140‑1 (MQ=255)                                                                               gcggcgCGTACAGAAGCCAGATCATTATAAGTACAGGTTAAGGTATATTTGGCGAAATATGCCGGAACGCCCGGCGAGTTTGGCTGGCCTAACGTGAGTGCGCCAGAACCGGCTTTCACCAGCAGGCAGTCAGCGTGAcc                                                        <  1:523243/140‑1 (MQ=255)                                                                                cggcgCGTACAGAAGCCAGATCATTATAAGTACAGGTTAAGGTATATTTGGCGAAATATGCCGGAACGCCCGGCGAGTTTGGCTGGCCTAACGTGAGTGCGCCAGAACCGGCTTTCACCAGCAGGCAGTCAGCGTGACCa                                                       >  1:308958/1‑140 (MQ=255)                                                                                  gcgcGTACAGAAGCCAGATCATTATAAGTACAGGTTAAGGTATATTTGGCGAAATATGCCGGAACGCCCGGCGAGTTTGGCTGGCCTAACGTGAGTGCGCCAGAACCGGCTTTCACCAGCAGGCAGTCAg                                                               >  1:1372111/1‑130 (MQ=255)                                                                                  gcgcGTACAGAAGCCAGATCATTATAAGTACAGGTTAAGGTATATTTGGCGAAATATGCCGGAACGCCCGGCGAGTTTGGCTGGCCTAACGTGAGTGCGCCAGAACCGGCTTTCACCAGCAGGCAGTCAg                                                               <  2:1372111/130‑1 (MQ=255)                                                                                     cgTACAGAAGCCAGATCATTATAAGTACAGGTTAAGGTATATTTGGCGAAATATGCCGGAACGCCCGGCGAGTTTGGCTGGCCTAACGTGAGTGCGCCAGAACCGGCTTTCACCAGCAGGCAGTCAGCGTGACCATGGTa                                                  >  2:918781/1‑140 (MQ=255)                                                                                       tACAGAAGCCAGATCATTATAAGTACAGGTTAAGGTATATTTGGCGAAATATGCCGGAACGCCCGGCGAGTTTGGCTGGCCTAACGTGAGTGCGCCAGAACCGGCTTTCACCAGCAGGCAGTCAGCGTGACCATGGTAac                                                <  1:996618/140‑1 (MQ=255)                                                                                        aCAGAAGCCAGATCATTATAAGTACAGGTTAAGGTATATTTGGCGAAATATGCCGGAACGCCCGGCGAGTTTGGCTGGCCTAACGTGAGTGCGCCAGAACCGGCTTTCACCAGCAGGCAGTCAGCGTGACCATGGTAaca                                               >  2:461500/1‑140 (MQ=255)                                                                                                  gATCATTATAAGTACAGGTTAAGGTATATTTGGCGAAATATGCCGGAACGCCCGGCGAGTTTGGCTGGCCTAACGTGAGTGCGCCAGAACCGGCTTTCACCAGCAGGCAGTCAGCGTGACCATGGTAACACCCTTCAAAt                                     >  1:713485/1‑140 (MQ=255)                                                                                                    tCATTATAAGTACAGGTTAAGGTATATTTGGCGAAATATGCCGGAACGCCCGGCGAGTTTGGCTGGCCTAACGTGAGTGCGCCAGAACCGGCTTTCACCAGCAGGCAGTCAGCGTGACCATGGTaa                                                 >  1:604937/1‑126 (MQ=255)                                                                                                    tCATTATAAGTACAGGTTAAGGTATATTTGGCGAAATATGCCGGAACGCCCGGCGAGTTTGGCTGGCCTAACGTGAGTGCGCCAGAACCGGCTTTCACCAGCAGGCAGTCAGCGTGACCATGGTaa                                                 <  2:604937/126‑1 (MQ=255)                                                                                                    tCATTATAAGTACAGGTTAAGGTATATTTGGCGAAATATGCCGGAACGCCCGGCGAGTTTGGCTGGCCTAACGTGAGTGCGCCAGAACCGGCTTTCACCAGCAGGCAGTCAGCGTGACCATGGTAACACCCTTCAAAttt                                   >  1:753866/1‑140 (MQ=255)                                                                                                      aTTATAAGTACAGGTTAAGGTATATTTGGCGAAATATGCCGGAACGCCCGGCGAGTTTGGCTGGCCTAACGTGAGTGCGCCAGAACCGGCTTTCACCAGCAGGCAGTCAGCGTGACCATGGTAACACCCTTCAAATTtaa                                 >  1:638949/1‑140 (MQ=255)                                                                                                        tataAGTACAGGTTAAGGTATATTTGGCGAAATATGCCGGAACGCCCGGCGAGTTTGGCTGGCCTAACGTGAGTGCGCCAGAACCGGCTTTCACCAGCAGGCAGTCAGCGTGACCATGGTAACACCCTTCAAATTtaata                               >  1:1270395/1‑140 (MQ=255)                                                                                                             gTACAGGTTAAGGTATATTTGGCGAAATATGCCGGAACGCCCGGCGAGTTTGGCTGGCCTAACGTGAGTGCGCCAGAACCGGCTTTCACCAGCAGGCAGTCAGCGTGACCATGGTAACACCCTTCAAATTTAATAAtttt                          >  1:348518/1‑140 (MQ=255)                                                                                                             gTACAGGTTAAGGTATATTTGGCGAAATATGCCGGAACGCCCGGCGAGTTTGGCTGGCCTAACGTGAGTGCGCCAGAACCGGCTTTCACCAGCAGGCAGTCAGCGTGACCATGGTAACACCCTTCAAATTTAATAAtttt                          >  2:1158747/1‑140 (MQ=255)                                                                                                               aCAGGTTAAGGTATATTTGGCGAAATATGCCGGAACGCCCGGCGAGTTTGGCTGGCCTAACGTGAGTGCGCCAGAACCGGCTTTCACCAGCAGGCAGTCAGCGTGACCATGGTAACACCCTTCAAATTTAATAATTTTGt                        >  1:479596/1‑140 (MQ=255)                                                                                                                 aGGTTAAGGTATATTTGGCGAAATATGCCGGAACGCCCGGCGAGTTTGGCTGGCCTAACGTGAGTGCGCCAGAACCGGCTTTCACCAGCAGGCAGTCAGCGTGACCATGGTAACACCCTTCAAATTTAATAATTTTGTcg                      >  2:86591/1‑140 (MQ=255)                                                                                                                  ggTTAAGGTATATTTGGCGAAATATGCCGGAACGCCCGGCGAGTTTGGCTGGCCTAACGTGAGTGCGCCAGAACCGGCTTTCACCAGCAGGCAGTCAGCGTGACCATGGTAACACCCTTCAAATTTAATAATTTTGTcgc                     >  1:294801/1‑140 (MQ=255)                                                                                                                  ggTTAAGGTATATTTGGCGAAATATGCCGGAACGCCCGGCGAGTTTGGCTGGCCTAACGTGAGTGCGCCAGAACCGGCTTTCACCAGCAGGCAGTCAGCGTGACCATGGTAACACCCTTCAAATTTAATAATTTTGTcgc                     >  2:221914/1‑140 (MQ=255)                                                                                                                   gTTAAGGTATATTTGGCGAAATATGCCGGAACGCCCGGCGAGTTTGGCTGGCCTAACGTGAGTGCGCCAGAACCGGCTTTCACCAGCAGGCAGTCAGCGTGACCATGGTAACACCCTTCAAATTTAATAATTTTGTcgcg                    >  2:515034/1‑140 (MQ=255)                                                                                                                   gTTAAGGTATATTTGGCGAAATATGCCGGAACGCCCGGCGAGTTTGGCTGGCCTAACGTGAGTGCGCCAGAACCGGCTTTCACCAGCAGGCAGTCAGCGTGACCATGGTAACACCCTTCAAATTTAATAATTTTGTcgcg                    <  2:1270395/140‑1 (MQ=255)                                                                                                                    ttAAGGTATATTTGGCGAAATATGCCGGAACGCCCGGCGAGTTTGGCTGGCCTAACGTGAGTGCGCCAGAACCGGCTTTCACCAGCAGGCAGTCAGCGTGACCATGGTAACACCCTTCAAATTTAATAATTTTGTCGCGa                   <  1:1158747/140‑1 (MQ=255)                                                                                                                     tAAGGTATATTTGGCGAAATATGCCGGAACGCCCGGCGAGTTTGGCTGGCCTAACGTGAGTGCGCCAGAACCGGCTTTCACCAGCAGGCAGTCAGCGTGACCATGGTAACACCCTTCAAATTTAATAATTTTGTCGCGAc                  <  1:221914/140‑1 (MQ=255)                                                                                                                      aaGGTATATTTGGCGAAATATGCCGGAACGCCCGGCGAGTTTGGCTGGCCTAACGTGAGTGCGCCAGAACCGGCTTTCACCAGCAGGCAGTCAGCGTGACCATGGTAACACCCTTCAAATTTAATAATTTTGTCGCGAcc                 >  1:198853/1‑140 (MQ=255)                                                                                                                             atTTGGCGAAATATGCCGGAACGCCCGGCGAGTTTGGCTGGCCTAACGTGAGTGCGCCAGAACCGGCTTTCACCAGCAGGCAGTCAGCGTGACCATGGTAACACCCTTCAAATTTAATAATTTTGTCGCGACCGGTaaaa          >  2:1416111/1‑140 (MQ=255)                                                                                                                              tttGGCGAAATATGCCGGAACGCCCGGCGAGTTTGGCTGGCCTAACGTGAGTGCGCCAGAACCGGCTTTCACCAGCAGGCAGTCAGCGTGACCATGGTAACACCCTTCAAATTTAATAATTTTGTCGCGACCGGTAAAAc         <  1:649679/140‑1 (MQ=255)                                                                                                                               ttGGCGAAATATGCCGGAACGCCCGGCGAGTTTGGCTGGCCTAACGTGAGTGCGCCAGAACCGGCTTTCACCAGCAGGCAGTCAGCGTGACCATGGTAACACCCTTCAAATTTAATAATTTTGTCGCGACCGGTAAAAcc        <  2:753866/140‑1 (MQ=255)                                                                                                                                tGGCGAAATATGCCGGAACGCCCGGCGAGTTTGGCTGGCCTAACGTGAGTGCGCCAGAACCGGCTTTCACCAGCAGGCAGTCAGCGTGACCATGGTAACACCCTTCAAATTTAATAATTTTGTCGCGACCGGTAAAACCa       <  2:192346/140‑1 (MQ=255)                                                                                                                                tGGCGAAATATGCCGGAACGCCCGGCGAGTTTGGCTGGCCTAACGTGAGTGCGCCAGAACCGGCTTTCACCAGCAGGCAGTCAGCGTGACCATGGTAACACCCTTCAAATTTAATAATTTTGTCGCGACCGGTAAAACCa       <  2:1338984/140‑1 (MQ=255)                                                                                                                                 ggCGAAATATGCCGGAACGCCCGGCGAGTTTGGCTGGCCTAACGTGAGTGCGCCAGAACCGGCTTTCACCAGCAGGCAGTCAGCGTGACCATGGTAACACCCTTCAAATTTAATAATTTTGTCGCGACCGGTAAAACCAc      <  2:315741/140‑1 (MQ=255)                                                                                                                                 ggCGAAATATGCCGGAACGCCCGGCGAGTTTGGCTGGCCTAACGTGAGTGCGCCAGAACCGGCTTTCACCAGCAGGCAGTCAGCGTGACCATGGTAACACCCTTCAAATTTAATAATTTTGTCGCGACCGGTAAAACCAc      >  2:1244203/1‑140 (MQ=255)                                                                                                                                   cGAAATATGCCGGAACGCCCGGCGAGTTTGGCTGGCCTAACGTGAGTGCGCCAGAACCGGCTTTCACCAGCAGGCAGTCAGCGTGACCATGGTAACACCCTTCAAATTTAATAATTTTGTCGCGACCGGTAAAACCACgg    >  2:720193/1‑140 (MQ=255)                                                                                                                                   cGAAATATGCCGGAACGCCCGGCGAGTTTGGCTGGCCTAACGTGAGTGCGCCAGAACCGGCTTTCACCAGCAGGCAGTCAGCGTGACCATGGTAACACCCTTCAAATTTAATAATTTTGTCGCGACCGGTAAAACCACgg    >  1:493880/1‑140 (MQ=255)                                                                                                                                   cGAAATATGCCGGAACGCCCGGCGAGTTTGGCTGGCCTAACGTGAGTGCGCCAGAACCGGCTTTCACCAGCAGGCAGTCAGCGTGACCATGGTAACACCCTTCAAATTTAATAATTTTGTCGCGACCGGTAAAACCACgg    >  2:1394206/1‑140 (MQ=255)                                                                                                                                    gAAATATGCCGGAACGCCCGGCGAGTTTGGCTGGCCTAACGTGAGTGCGCCAGAACCGGCTTTCACCAGCAGGCAGTCAGCGTGACCATGGTAACACCCTTCAAATTTAATAATTTTGTCGCGACCAGTAAAACCACggg   >  1:1352683/1‑140 (MQ=255)                                                                                                                                     aaaTATGCCGGAACGCCCGGCGAGTTTGGCTGGCCTAACGTGAGTGCGCCAGAACCGGCTTTCACCAGCAGGCAGTCAGCGTGACCATGGTAACACCCTTCAAATTTAATAATTTTGTCGCGACCGGTAAAACCACGGGc  <  2:294801/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                        AGCGGCGGAACACAGTTCATATTGCCTGCCACCGGCTCGACGATAATACAGGCAATCTCTTGCGGGTATTGCTCAAATGCGGCGCGTACAGAAGCCAGATCATTATAAGTACAGGTTAAGGTATATTTGGCGAAATCTGCCGGAACGCCCGGCGAGTTTGGCTGGCCTAACGTGAGTGCGCCAGAACCGGCTTTCACCAGCAGGCAGTCAGCGTGACCATGGTAACACCCTTCAAATTTAATAATTTTGTCGCGACCGGTAAAACCACGGGC  >  NC_000913/174236‑174507

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑