BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,261,710	C→A	S14R (AGC→AGA)	dusA →	tRNA‑dihydrouridine synthase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,261,710	0	C	A	100.0%	606.9 / NA	170	S14R (AGC→AGA)	dusA	tRNA‑dihydrouridine synthase A
Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (89/81); total (89/81)

Only 100 of 173 total aligned reads displayed.

GTTATGGTATAGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGCGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATACGTTGCTGTATACCGAAATGGT  >  NC_000913/4261573‑4261844

gttATGGTATAGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAgagc                                                                                                                                      >  2:10627/1‑140 (MQ=255)

         taGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGa                                                                                                                             >  1:432829/1‑140 (MQ=255)

            tACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTATATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGaaaa                                                                                                                          >  1:987523/1‑140 (MQ=255)

              ccccGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGaaaaaa                                                                                                                        >  2:198439/1‑140 (MQ=255)

                 cGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATTCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGa                                                                                                                             <  1:1272148/132‑1 (MQ=255)

                 cGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTg                                                                                                                     >  2:841694/1‑140 (MQ=255)

                 cGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTg                                                                                                                     >  2:1035043/1‑140 (MQ=255)

                 cGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTg                                                                                                                     >  1:757165/1‑140 (MQ=255)

                   cTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGAc                                                                                                                   >  1:1006557/1‑140 (MQ=255)

                   cTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGAc                                                                                                                   <  1:1445012/140‑1 (MQ=255)

                      ttGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGtt                                                                                                                <  2:732276/140‑1 (MQ=255)

                                  aaTAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGt                                                                                                    <  2:1398115/140‑1 (MQ=255)

                                  aaTAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGt                                                                                                    <  1:268956/140‑1 (MQ=255)

                                       tGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGtt                                                                                               >  2:1002299/1‑140 (MQ=255)

                                       tGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGtt                                                                                               >  2:249707/1‑140 (MQ=255)

                                          tGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTATATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAg                                                                                            <  2:987523/140‑1 (MQ=255)

                                          tGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAg                                                                                            <  2:636934/140‑1 (MQ=255)

                                           gCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGc                                                                                           >  1:112260/1‑140 (MQ=255)

                                            cTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGt                                                                                                    >  1:227954/1‑130 (MQ=255)

                                            cTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGt                                                                                                    <  2:227954/130‑1 (MQ=255)

                                            cTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCg                                                                                          >  2:209716/1‑140 (MQ=255)

                                            cTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCg                                                                                          >  2:1070250/1‑140 (MQ=255)

                                            cTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCg                                                                                          >  2:1211319/1‑140 (MQ=255)

                                               aaTAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGttt                                                                                              <  1:12466/133‑1 (MQ=255)

                                               aaTAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGttt                                                                                              >  2:12466/1‑133 (MQ=255)

                                                 taacataacCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCa                                                                                     >  1:263571/1‑140 (MQ=255)

                                                  aacataacCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCAc                                                                                    <  1:10627/140‑1 (MQ=255)

                                                  aacataacCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCAc                                                                                    <  1:1219311/140‑1 (MQ=255)

                                                      taacCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCACCAAt                                                                                >  2:1305864/1‑140 (MQ=255)

                                                         ccccATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCt                                                                             >  2:1080455/1‑140 (MQ=255)

                                                         ccccATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCt                                                                             <  2:1458567/140‑1 (MQ=255)

                                                         ccccATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCt                                                                             >  2:964391/1‑140 (MQ=255)

                                                           ccATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCg                                                                           >  2:30855/1‑140 (MQ=255)

                                                              tGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCAc                                                                                    >  1:743176/1‑128 (MQ=255)

                                                              tGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACt                                                                        >  1:258121/1‑140 (MQ=255)

                                                              tGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACt                                                                        >  1:201611/1‑140 (MQ=255)

                                                              tGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACt                                                                        >  1:1371939/1‑140 (MQ=255)

                                                                atatatCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTgg                                                                      <  1:1241883/140‑1 (MQ=255)

                                                                 tatatCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTgga                                                                     <  2:11190/140‑1 (MQ=255)

                                                                   tatCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTggacg                                                                   >  1:168042/1‑140 (MQ=255)

                                                                    atCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTgga                                                                     <  2:495613/137‑1 (MQ=255)

                                                                      cGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCACCAATg                                                                               >  2:625460/1‑125 (MQ=255)

                                                                      cGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCACCAATg                                                                               <  1:625460/125‑1 (MQ=255)

                                                                      cGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTggacggac                                                                >  1:533164/1‑140 (MQ=255)

                                                                       gATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGgaca                                                               <  1:1409809/140‑1 (MQ=255)

                                                                        aTAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTggacgg                                                                  >  2:1022573/1‑136 (MQ=255)

                                                                        aTAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTgga                                                                     >  2:443895/1‑133 (MQ=255)

                                                                        aTAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGgacag                                                              >  1:405737/1‑140 (MQ=255)

                                                                        aTAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGgacag                                                              >  1:378738/1‑140 (MQ=255)

                                                                          aaaaTAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTgga                                                                     >  2:1330003/1‑131 (MQ=255)

                                                                          aaaaTAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGgacaga                                                             >  1:490595/1‑139 (MQ=255)

                                                                           aaaTAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTgg                                                                      >  1:525182/1‑129 (MQ=255)

                                                                           aaaTAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTgg                                                                      <  2:519815/129‑1 (MQ=255)

                                                                           aaaTAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTgg                                                                      >  1:519815/1‑129 (MQ=255)

                                                                            aataatCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACAt                                                          <  2:757165/140‑1 (MQ=255)

                                                                              taatCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTg                                                        <  2:1129397/140‑1 (MQ=255)

                                                                               aatCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACAt                                                          <  1:664850/137‑1 (MQ=255)

                                                                                atCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTg                                                        >  1:112984/1‑138 (MQ=255)

                                                                                atCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTg                                                        <  2:112984/138‑1 (MQ=255)

                                                                                      aCATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTAtt                                                >  1:791702/1‑140 (MQ=255)

                                                                                       cATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTAttt                                               >  1:1431500/1‑140 (MQ=255)

                                                                                         tttGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCt                                             >  1:1025914/1‑140 (MQ=255)

                                                                                          ttGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGc                                                    >  2:920988/1‑132 (MQ=255)

                                                                                          ttGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCtt                                            <  1:871348/140‑1 (MQ=255)

                                                                                          ttGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCtt                                            >  1:929251/1‑140 (MQ=255)

                                                                                          ttGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCtt                                            >  2:558783/1‑140 (MQ=255)

                                                                                               aaTGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTc                                       <  2:929251/140‑1 (MQ=255)

                                                                                                 tGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTg                                     >  1:579214/1‑140 (MQ=255)

                                                                                                   cACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCt                                   <  1:727780/140‑1 (MQ=255)

                                                                                                    aCGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCtt                                  >  2:215726/1‑140 (MQ=255)

                                                                                                      ggTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTc                                >  1:1018875/1‑140 (MQ=255)

                                                                                                      ggTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTc                                <  2:405737/140‑1 (MQ=255)

                                                                                                           ttCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCa                           <  1:1462895/140‑1 (MQ=255)

                                                                                                           ttCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCa                           <  2:19703/140‑1 (MQ=255)

                                                                                                              tGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATa                        <  2:1006557/140‑1 (MQ=255)

                                                                                                              tGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATa                        <  1:1330819/140‑1 (MQ=255)

                                                                                                                aaaTGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATACg                      >  2:1263513/1‑140 (MQ=255)

                                                                                                                aaaTGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATACg                      >  2:1358703/1‑140 (MQ=255)

                                                                                                                aaaTGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATACg                      <  2:680540/140‑1 (MQ=255)

                                                                                                                  aTGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATACGtt                    >  1:1323126/1‑140 (MQ=255)

                                                                                                                   tGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATACGTTg                   <  2:378738/140‑1 (MQ=255)

                                                                                                                   tGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATACGTTg                   <  1:1211319/140‑1 (MQ=255)

                                                                                                                   tGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATACGTTg                   <  1:1336038/140‑1 (MQ=255)

                                                                                                                   tGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATACGTTg                   <  2:1396181/140‑1 (MQ=255)

                                                                                                                   tGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATACGTTg                   <  1:1035043/140‑1 (MQ=255)

                                                                                                                     cAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATACGTTGCt                 <  2:1431500/140‑1 (MQ=255)

                                                                                                                     cAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATACGTTGCt                 <  1:209716/140‑1 (MQ=255)

                                                                                                                     cAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATACGTTGCt                 <  2:11986/140‑1 (MQ=255)

                                                                                                                     cAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATACGTTGCt                 <  1:353305/140‑1 (MQ=255)

                                                                                                                       aaagaTCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATACGTTGCTGt               >  1:922248/1‑140 (MQ=255)

                                                                                                                        aaaaTCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATACGTTGCTGta              <  1:558783/140‑1 (MQ=255)

                                                                                                                        aaaaTCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATACGTTGCTGta              <  2:734796/140‑1 (MQ=255)

                                                                                                                        aaaaTCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATACGTTGCTGta              <  2:579214/140‑1 (MQ=255)

                                                                                                                          aaTCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATACGTTGCTGtata            <  1:1305864/140‑1 (MQ=255)

                                                                                                                               accaaaccaGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATACGTTGCTGTATACCGaa       <  2:1339473/140‑1 (MQ=255)

                                                                                                                                  aaaccaGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATACGTTGCTGTATACCGAAATg    <  1:198439/140‑1 (MQ=255)

                                                                                                                                  aaaccaGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATACGTTGCTGTATACCGAAATg    <  1:1002299/140‑1 (MQ=255)

                                                                                                                                  aaaccaGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATACGTTGCTGTATACCGAAATg    <  2:262950/140‑1 (MQ=255)

                                                                                                                                    accaGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATACGTTGCTGTATACCGAAATGGt  <  2:1218573/140‑1 (MQ=255)

                                                                                                                                    accaGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATACGTTGCTGTATACCGAAATGGt  <  1:1114582/140‑1 (MQ=255)

GTTATGGTATAGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGCGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATACGTTGCTGTATACCGAAATGGT  >  NC_000913/4261573‑4261844

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`