Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,489,200	C→A	R288S (AGG→AGT)	yjgR ←	DUF853 domain‑containing protein YjgR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,489,200	0	C	A	100.0%	236.6 / NA	68	R288S (AGG→AGT)	yjgR	DUF853 domain‑containing protein YjgR
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (36/32);  total (36/32)

TTTCTGATCTTTTGGCGTAAAAGCCCGCAAAGCATGTTGAACGCGATTACCGAGCTGCCCGAGCACATTATCCGGAATATCAGACGGGTTTTGCGAAACGAACCAGACGCCCACGCCTTTTGAGCGAATAAGCCTTATCACCTGCTCAATCTTATCCAGCAGTACCTGCGGGGCATCGTTAAACAGCAGATGTGCCTCGTCGAAGAAAAACACCAGTTTTGGTTTCTCCAGATCGCCCGCTTCCGGCAATTGTTCATACAACTCTGAAA  >  NC_000913/4489068‑4489336                                                                                                                                     |                                                                                                                                         tttCTGATCTTTTGGCGTAAAAGCCCGCAAAGCATGTTGAACGCGATTACCGAGCTGCCCGAGCACATTATCCGGAATATCAGACGGGTTTTGCGAAACGAACCAGACGCCCACGCCTTTTGAGCGAATAAGACTTATCa                                                                                                                                   >  2:1286543/1‑140 (MQ=255)   tCTGATCTTTTGGCGTAAAAGCCCGCAAAGCATGTTGAACGCGATTACCGAGCTGCCCGAGCACATTATCCGGAATATCAGACGGGTTTTGCGGAACGAACCAGACGCCCACGCCTTTTGAGCGAATAAGACTTATCAcc                                                                                                                                 >  2:1341822/1‑140 (MQ=255)     tGATCTTTTGGCGTAAAAGCCCGCAAAGCATGTTGAACGCGATTACCGAGCTGCCCGAGCACATTATCCGGAATATCAGACGGGTTTTGCGAAACGAACCAGACGCCCACGCCTTTTGAGCGAATAAGACTTATCACCTg                                                                                                                               >  1:378128/1‑140 (MQ=255)       aTCTTTTGGCGTAAAAGCCCGCAAAGCATGTTGAACGCGATTACCGAGCTGCCCGAGCACATTATCCGGAATATCAGACGGGTTTTGCGAAACGAACCAGACGCCCACGCCTTTTGAGCGAATAAGACTTATCACCTGCt                                                                                                                             <  2:378128/140‑1 (MQ=255)       aTCTTTTGGCGTAAAAGCCCGCAAAGCATGTTGAACGCGATTACCGAGCTGCCCGAGCACATTATCCGGAATATCAGACGGGTTTTGCGAAACGAACCAGACGCCCACGCCTTTTGAGCGAATAAGACTTATCACCTGCt                                                                                                                             <  2:714846/140‑1 (MQ=255)        tCTTTTGGCGTAAAAGCCCGCAAAGCATGTTGAACGCGATTACCGAGCTGCCCGAGCACATTATCCGGAATATCAGACGGGTTTTGCGAAACGAACCAGACGCCCACGCCTTTTGAGCGAATAAGACTTATCACCTGCTc                                                                                                                            >  1:204300/1‑140 (MQ=255)            ttGGCGTAAAAGCCCGCAAAGCATGTTGAACGCGATTACCGAGCTGCCCGAGCACATTATCCGGAATATCAGACGGGTTTTGCGAAACGAACCGGACGCCCACGCCTTTTGAGCGAATAAGACTTATCACCTGCTCAATc                                                                                                                        <  2:498332/140‑1 (MQ=255)                cGTAAAAGCCCGCAAAGCATGTTGAACGCGATTACCGAGCTGCCCGAGCACATTATCCGGAATATCAGACGGGTTTTGCGAAACGAACCAGACGCCCACGCCTTTTGAGCGAATAAGACTTATCACCTGCTCAATCTTAt                                                                                                                    <  2:929321/140‑1 (MQ=255)                cGTAAAAGCCCGCAAAGCATGTTGAACGCGATTACCGAGCTGCCCGAGCACATTATCCGGAATATCAGACGGGTTTTGCGAAACGAACCAGACGCCCACGCCTTTTGAGCGAATAAGACTTATCACCTGCTCAATCTTAt                                                                                                                    <  1:511070/140‑1 (MQ=255)                  tAAAAGCCCGCAAAGCATGTTGAACGCGATTACCGAGCTGCCCGAGCACATTATCCGGAATATCAGACGGGTTTTGCGAAACGAACCAGACGCCCACGCCTTTTGAGCGAATAAGACTTATCACCTGCTCAATCTTATcc                                                                                                                  <  1:1431168/140‑1 (MQ=255)                    aaaGCCCGCAAAGCATGTTGAACGCGATTACCGAGCTGCCCGAGCACATTATCCGGAATATCAGACGGGTTTTGCGAAACGAACCAGACGCCCACGCCTTTTGAGCGAATAAGACTTATCACCTGCTCAATCTTATCcag                                                                                                                >  1:51865/1‑140 (MQ=255)                      aGCCCGCAAAGCATGTTGAACGCGATTACCGAGCTGCCCGAGCACATTATCCGGAATATCAGACGGGTTTTGCGAAACGAACCAGACGCCCACGCCTTTTGAGCGAATAAGACTTATCACCTGCTCAATCTTATCcagca                                                                                                              <  1:909872/140‑1 (MQ=255)                       gCCCGCAAAGCATGTTGAACGCGATTACCGAGCTGCCCGAGCACATTATCCGGAATATCAGACGGGTTTTGCGAAACGCACCAGACGCCCACGCCTTTTGAGCGAATAAGACTTATCACCTGCTCAATCTTATCcagcag                                                                                                             <  2:1139669/140‑1 (MQ=255)                          cGCAAAGCATGTTGAACGCGATTACCGAGCTGCCCGAGCACATTATCCGGAATATCAGACGGGTTTTGCGAAACGAACCAGACGCCCACGCCTTTTGAGCGAATAAGACTTATCACCTGCTCAATc                                                                                                                        <  1:390519/126‑1 (MQ=255)                          cGCAAAGCATGTTGAACGCGATTACCGAGCTGCCCGAGCACATTATCCGGAATATCAGACGGGTTTTGCGAAACGAACCAGACGCCCACGCCTTTTGAGCGAATAAGACTTATCACCTGCTCAATc                                                                                                                        >  2:390519/1‑126 (MQ=255)                          cGCAAAGCATGTTGAACGCGATTACCGAGCTGCCCGAGCACATTATCCGGAATATCAGACGGGTTTTGCGAAACGAACCAGACGCCCACGCCTTTTGAGCGAATAAGACTTATCACCTGCTCAATCTTATCCAGCAGTAc                                                                                                          >  1:876994/1‑140 (MQ=255)                                  aTGTTGAACGCGATTACCGAGCTGCCCGAGCACATTATCCGGAATATCAGACGGGTTTTGCGGAACGAACCAGACGCCCACGCCTTTTGAGCGAATAAGACTTATCACCTGCTCAATCTTATCCAGCAGTACCTGCgggg                                                                                                  <  1:1341822/140‑1 (MQ=255)                                     ttGAACGCGATTACCGAGCTGCCCGAGCACATTATCCGGAATATCAGACGGGTTTTGCGAAACGAACCAGACGCCCACGCCTTTTGAGCGAATAAGACTTATCACCTGCTCAATCTTATCCAGCAGTACCTGCGGGGCAt                                                                                               <  1:1334363/140‑1 (MQ=255)                                         aCGCGATTACCGAGCTGCCCGAGCACATTATCCGGAATATCAGACGGGTTTTGCGAAACGAACCAGACGCCCACGCCTTTTGAGCGAATAAGACTTATCACCTGCTCAATCTTATCCAGCAGTACCTGCGGGGc                                                                                                 >  1:1398308/1‑134 (MQ=255)                                         aCGCGATTACCGAGCTGCCCGAGCACATTATCCGGAATATCAGACGGGTTTTGCGAAACGAACCAGACGCCCACGCCTTTTGAGCGAATAAGACTTATCACCTGCTCAATCTTATCCAGCAGTACCTGCGGGGc                                                                                                 <  2:1398308/134‑1 (MQ=255)                                         aCGCGATTACCGAGCTGCCCGAGCACATTATCCGGAATATCAGACGGGTTTTGCGAAACGAACCAGACGCCCACGCCTTTTGAGCGAATAAGACTTATCACCTGCTCAATCTTATCCAGCAGTACCTGCGGGGCATCGtt                                                                                           >  1:381464/1‑140 (MQ=255)                                           gcgATTACCGAGCTGCCCGAGCACATTATCCGGAATATCAGACGGGTTTTGCGAAACGAACCAGACGCCCACGCCTTTTGAGCGAATAAGACTTATCACCTGCTCAATCTTATCCAGCAGTACCTGCGGGGCATCGTTaa                                                                                         >  1:1374764/1‑140 (MQ=255)                                           gcgATTACCGAGCTGCCCGAGCACATTATCCGGAATATCAGACGGGTTTTGCGAAACGAACCAGACGCCCACGCCTTTTGAGCGAATAAGACTTATCACCTGCTCAATCTTATCCAGCAGTACCTGCGGGGCATCGTTaa                                                                                         >  1:86155/1‑140 (MQ=255)                                              aTTACCGAGCTGCCCGAGCACATTATCCGGAATATCAGACGGGTTTTGCGAAACGAACCAGACGCCCACGCCTTTTGAGCGAATAAGACTTATCACCTGCTCAATCTTATCCAGCAGTACCTGCGGGGCATCGTTAAAca                                                                                      >  1:1280985/1‑140 (MQ=255)                                              aTTACCGAGCTGCCCGAGCACATTATCCGGAATATCAGACGGGTTTTGCGAAACGAACCAGACGCCCACGCCTTTTGAGCGAATAAGACTTATCACCTGCTCAATCTTATCCAGCAGTACCTGCGGGGCATCGTTAAAca                                                                                      <  2:381464/140‑1 (MQ=255)                                               ttACCGAGCTGCCCGAGCACATTATCCGGAATATCAGACGGGTTTTGCGAAACGAACCAGACGCCCACGCCTTTTGAGCGAATAAGACTTATCACCTGCTCAATCTTATCCAGCAGTACCTGCGGGGCATCGTTAAAcag                                                                                     <  2:427361/140‑1 (MQ=255)                                                     aGCTGCCCGAGCACATTATCCGGAATATCAGACGGGTTTTGCGAAACGAACCAGACGCCCACGCCTTTTGAGCGAATAAGACTTATCACCTGCTCAATCTTATCCAGCAGTACCTGCGGGGCATCGTTAAACAGCAGAtg                                                                               >  2:820533/1‑140 (MQ=255)                                                          cccGAGCACATTATCCGGAATATCAGACGGGTTTTGCGAAACGAACCAGACGCCCACGCCTTTTGAGCGAATAAGACTTATCACCTGCTCAATCTTATCCAGCAGTACCTGCGGGGCATCGTTAAACAGCAGATGTGCCt                                                                          >  2:395450/1‑140 (MQ=255)                                                                 acaTTATCCGGAATATCAGACGGGTTTTGCGAAACGAACCAGACGCCCACGCCTTTTGAGCGAATAAGACTTATCACCTGCTCAATCTTATCCAGCAGTACCTGCGGGGCATCGTTAAACAGCAGATGTGCCTCGTCgaa                                                                   >  1:1323381/1‑140 (MQ=255)                                                                         cGGAATATCAGACGGGTTTTGCGAAACGAACCAGACGCCCACGCCTTTTGAGCGAATAAGACTTATCACCTGCTCAATCTTATCCAGCAGTACCTGCGGGGCATCGTTAAACAGCAGATGTGCCTCGTCGAAGAAAAaca                                                           >  2:16972/1‑140 (MQ=255)                                                                         cGGAATATCAGACGGGTTTTGCGAAACGAACCAGACGCCCACGCCTTTTGAGCGAATAAGACTTATCACCTGCTCAATCTTATCCAGCAGTACCTGCGGGGCATCGTTAAACAGCAGATGTGCCTCGTCGAAGAAAAaca                                                           >  1:1350460/1‑140 (MQ=255)                                                                          ggAATATCAGACGGGTTTTGCGAAACGAACCAGACGCCCACGCCTTTTGAGCGAATAAGACTTATCACCTGCTCAATCTTATCCAGCAGTACCTGCGGGGCATCGTTAAACAGCAGATGTGCCTCGTCGAAGAAAAacac                                                          <  2:86155/140‑1 (MQ=255)                                                                             atatCAGACGGGTTTTGCGAAACGAACCAGACGCCCACGCCTTTTGAGCGAATAAGACTTATCACCTGCTCAATCTTATCCAGCAGTACCTGCGGGGCATCGTTAAACAGCAGATGTGCCTCGTCGAAGAAAAACACCAg                                                       >  1:375244/1‑140 (MQ=255)                                                                             atatCAGACGGGTTTTGCGAAACGAACCAGACGCCCACGCCTTTTGAGCGAATAAGACTTATCACCTGCTCAATCTTATCCAGCAGTACCTGCGGGGCATCGTTAAACAGCAGATGTGCCTCGTCGAAGAAAAACACCAg                                                       >  1:163334/1‑140 (MQ=255)                                                                                 cAGACGGGTTTTGCGAAACGAACCAGACGCCCACGCCTTTTGAGCGAATAAGACTTATCACCTGCTCAATCTTATCCAGCAGTACCTGCGGGGCATCGTTAAACAGCAGATGTGCCTCGTCGAAGAAAAACACCAGtttt                                                   <  2:1280985/140‑1 (MQ=255)                                                                                 cAGACGGGTTTTGCGAAACGAACCAGACGCCCACGCCTTTTGAGCGAATAAGACTTATCACCTGCTCAATCTTATCCAGCAGTACCTGCGGGGCATCGTTAAACAGCAGATGTGCCTCGTCGAAGAAAAACACCAGtttt                                                   >  1:1273249/1‑140 (MQ=255)                                                                                 cAGACGGGTTTTGCGAAACGAACCAGACGCCCACGCCTTTTGAGCGAATAAGACTTATCACCTGCTCAATCTTATCCAGCAGTACCTGCGGGGCATCGTTAAACAGCAGATGTGCCTCGTCGAAGAAAAACACCAGtttt                                                   >  1:1365014/1‑140 (MQ=255)                                                                                   gACGGGTTTTGCGAAACGAACCAGACGCCCACGCCTTTTGAGCGAATAAGACTTATCACCTGCTCAATCTTATCCAGCAGTACCTGCGGGGCATCGTTAAACAGCAGATGTGCCTCGTCGAAGAAAAACACCAGTTTTgg                                                 >  1:618842/1‑140 (MQ=255)                                                                                   gACGGGTTTTGCGAAACGAACCAGACGCCCACGCCTTTTGAGCGAATAAGACTTATCACCTGCTCAATCTTATCCAGCAGTACCTGCGGGGCATCGTTAAACAGCAGATGTGCCTCGTCGAAGAAAAACACCAGTTTTgg                                                 <  2:1375495/140‑1 (MQ=255)                                                                                    aCGGGTTTTGCGAAACGAACCAGACGCCCACGCCTTTTGAGCGAATAAGACTTATCACCTGCTCAATCTTATCCAGCAGTACCTGCGGGGCATCGTTAAACAGCAGATGTGCCTCGTCGAAGAAAAACACCAGTTTTGGt                                                <  2:1350460/140‑1 (MQ=255)                                                                                      gggTTTTGCGAAACGAACCAGACGCCCACGCCTTTTGAGCGAATAAGACTTATCACCTGCTCAATCTTATCCAGCAGTACCTGCGGGGCATCGTTAAACAGCAGATGTGCCTCGTCGAAGAAAAACACCAGTTTTGGttt                                              >  2:1401769/1‑140 (MQ=255)                                                                                        gTTTTGCGAAACGAACCAGACGCCCACGCCTTTTGAGCGAATAAGACTTATCACCTGCTCAATCTTATCCAGCAGTACCTGCGGGGCATCGTTAAACAGCAGATGTGCCTCGTCGAAGAAAAACACCAGTTTTgg                                                 <  1:670512/135‑1 (MQ=255)                                                                                        gTTTTGCGAAACGAACCAGACGCCCACGCCTTTTGAGCGAATAAGACTTATCACCTGCTCAATCTTATCCAGCAGTACCTGCGGGGCATCGTTAAACAGCAGATGTGCCTCGTCGAAGAAAAACACCAGTTTTgg                                                 >  2:670512/1‑135 (MQ=255)                                                                                        gTTTTGCGAAACGAACCAGACGCCCACGCCTTTTGAGCGAATAAGACTTATCACCTGCTCAATCTTATCCAGCAGTACCTGCGGGGCATCGTTAAACAGCAGATGTGCCTCGTCGAAGAAAAACACCAGTTTTGGTTtct                                            >  2:1222645/1‑140 (MQ=255)                                                                                                 aaCGAACCAGACGCCCACGCCTTTTGAGCGAATAAGACTTATCACCTGCTCAATCTTATCCAGCAGTACCTGCGGGGCATCGTTAAACAGCAGATGTGCCTCGTCGAAGAAAAACACCAGTTTTGGTTTCTCCAGATCGc                                   >  1:258911/1‑140 (MQ=255)                                                                                                  aCGAACCAGACGCCCACGCCTTTTGAGCGAATAAGACTTATCACCTGCTCAATCTTATCCAGCAGTACCTGCGGGGCATCGTTAAACAGCAGATGTGCCTCGTCGAAGAAAAACACCAGTTTTGGTTTCTCCAGATCGcc                                  >  1:808573/1‑140 (MQ=255)                                                                                                  aCGAACCAGACGCCCACGCCTTTTGAGCGAATAAGACTTATCACCTGCTCAATCTTATCCAGCAGTACCTGCGGGGCATCGTTAAACAGCAGATGTGCCTCGTCGAAGAAAAACACCAGTTTTGGTTTCTCCAGATCGcc                                  >  1:860948/1‑140 (MQ=255)                                                                                                    gAACCAGACGCCCACGCCTTTTGAGCGAATAAGACTTATCACCTGCTCAATCTTATCCAGCAGTACCTGCGGGGCATCGTTAAACAGCAGATGTGCCTCGTCGAAGAAAAACACCAGTTTTGGTTTCTCCAGATCGCCCg                                <  2:1323381/140‑1 (MQ=255)                                                                                                             gCCCACGCCTTTTGAGCGAATAAGACTTATCACCTGCTCAATCTTATCCAGCAGTACCTGCGGGGCATCGTTAAACAGCAGATGTGCCTCGTCGAAGAAAAACACCAGTTTTGGTTTCTCCAGATCGCCCGCTTCCGGCa                       <  1:1401769/140‑1 (MQ=255)                                                                                                              cccACGCCTTTTGAGCGAATAAGACTTATCACCTGCTCAATCTTATCCAGCAGTACCTGCGGGGCATCGTTAAACAGCAGATGTGCCTCGTCGAAGAAAAACACCAGTTTTGGTTTCTCCAGATCGCCCGCTTCCGGCaa                      <  2:204300/140‑1 (MQ=255)                                                                                                              cccACGCCTTTTGAGCGAATAAGACTTATCACCTGCTCAATCTTATCCAGCAGTACCTGCGGGGCATCGTTAAACAGCAGATGTGCCTCGTCGAAGAAAAACACCAGTTTTGGTTTCTCCAGATCGCCCGCTTCCGGCaa                      <  1:642839/140‑1 (MQ=255)                                                                                                                cACGCCTTTTGAGCGAATAAGACTTATCACCTGCTCAATCTTATCCAGCAGTACCTGCGGGGCATCGTTAAACAGCAGATGTGCCTCGTCGAAGAAAAACACCAGTTTTGGTTTCTCCAGATCGCCCGCTTCCGGCAAtt                    >  1:605319/1‑140 (MQ=255)                                                                                                                  cGCCTTTTGAGCGAATAAGACTTATCACCTGCTCAATCTTATCCAGCAGTACCTGCGGGGCATCGTTAAACAGCAGATGTGCCTCGTCGAAGAAAAACACCAGTTTTGGTTTCTCCAGATCGCCCGCTTCCGGCAATTGt                  <  1:99665/140‑1 (MQ=255)                                                                                                                       tttGAGCGAATAAGACTTATCACCTGCTCAATCTTATCCAGCAGTACCTGCGGGGCATCGTTAAACAGCAGATGTGCCTCGTCGAAGAAAAACACCAGTTTTGGTTTCTCCAGATCGCCCGCTTCCGGCAATTGTTCATa             <  1:1222645/140‑1 (MQ=255)                                                                                                                       tttGAGCGAATAAGACTTATCACCTGCTCAATCTTATCCAGCAGTACCTGCGGGGCATCGTTAAACAGCAGATGTGCCTCGTCGAAGAAAAACACCAGTTTTGGTTTCTCCAGATCGCCCGCTTCCGGCAATTGTTCATa             <  2:375244/140‑1 (MQ=255)                                                                                                                        ttGAGCGAATAAGACTTATCACCTGCTCAATCTTATCCAGCAGTACCTGCGGGGCATCGTTAAACAGCAGATGTGCCTCGTCGAAGAAAAACACCAGTTTTGGTTTCTCCAGATCGCCCGCTTCCGGCAATTGTTCATAc            >  1:188882/1‑140 (MQ=255)                                                                                                                         tGAGCGAATAAGACTTATCACCTGCTCAATCTTATCCAGCAGTACCTGCGGGGCATCGTTAAACAGCAGATGTGCCTCGTCGAAGAAAAACACCAGTTTTGGTTTCTCCAGATCGCCCGCTTCCGGCAATTGt                  <  1:778254/133‑1 (MQ=255)                                                                                                                         tGAGCGAATAAGACTTATCACCTGCTCAATCTTATCCAGCAGTACCTGCGGGGCATCGTTAAACAGCAGATGTGCCTCGTCGAAGAAAAACACCAGTTTTGGTTTCTCCAGATCGCCCGCTTCCGGCAATTGt                  >  2:778254/1‑133 (MQ=255)                                                                                                                           aGCGAATAAGACTTATCACCTGCTCAATCTTATCCAGCAGTACCTGCGGGGCATCGTTAAACAGCAGATGTGCCTCGTCGAAGAAAAACACCAGTTTTGGTTTCTCCAGATCGCCCGCTTCCGGCAATTGTTCATACAAc         >  2:1304224/1‑140 (MQ=255)                                                                                                                               aaTAAGACTTATCACCTGCTCAATCTTATCCAGCAGTACCTGCGGGGCATCGTTAAACAGCAGATGTGCCTCGTCGAAGAAAAACACCAGTTTTGGTTTCTCCAGATCGCCCGCTTCCGGCAAtt                    >  1:438801/1‑125 (MQ=255)                                                                                                                               aaTAAGACTTATCACCTGCTCAATCTTATCCAGCAGTACCTGCGGGGCATCGTTAAACAGCAGATGTGCCTCGTCGAAGAAAAACACCAGTTTTGGTTTCTCCAGATCGCCCGCTTCCGGCAAtt                    <  2:438801/125‑1 (MQ=255)                                                                                                                               aaTAAGACTTATCACCTGCTCAATCTTATCCAGCAGTACCTGCGGGGCATCGTTAAACAGCAGATGTGCCTCGTCGAAGAAAAACACCAGTTTTGGTTTCTCCAGATCGCCCGCTTCCGGCAATTGt                  >  1:781/1‑127 (MQ=255)                                                                                                                               aaTAAGACTTATCACCTGCTCAATCTTATCCAGCAGTACCTGCGGGGCATCGTTAAACAGCAGATGTGCCTCGTCGAAGAAAAACACCAGTTTTGGTTTCTCCAGATCGCCCGCTTCCGGCAATTGt                  <  2:781/127‑1 (MQ=255)                                                                                                                               aaTAAGACTTATCACCTGCTCAATCTTATCCAGCAGTACCTGCGGGGCATCGTTAAACAGCAGATGTGCCTCGTCGAAGAAAAACACCAGTTTTGGTTTCTCCAGATCGCCCGCTTCCGGCAATTGTTCATACAACTCTg     <  2:1374764/140‑1 (MQ=255)                                                                                                                               aaTAAGACTTATCACCTGCTCAATCTTATCCAGCAGTACCTGCGGGGCATCGTTAAACAGCAGATGTGCCTCGTCGAAGAAAAACACCAGTTTTGGTTTCTCCAGATCGCCCGCTTCCGGCAATTGTTCATACAACTCTg     >  2:1249597/1‑140 (MQ=255)                                                                                                                               aaTAAGACTTATCACCTGCTCAATCTTATCCAGCAGTACCTGCGGGGCATCGTTAAACAGCAGATGTGCCTCGTCGAAGAAAAACACCAGTTTTGGTTTCTCCAGATCGCCCGCTTCCGGCAATTGTTCATACAACTCTg     >  2:1060492/1‑140 (MQ=255)                                                                                                                                 tAAGACTTATCACCTGCTCAATCTTATCCAGCAGTACCTGCGGGGCATCGTTAAACAGCAGATGTGCCTCGTCGAAGAAAAACACCAGTTTTGGTTTCTCCAGATCGCCCGCTTCCGGCAATTGTTCATACAACTCTGaa   <  2:1273249/140‑1 (MQ=255)                                                                                                                                  aaGACTTATCACCTGCTCAATCTTATCCAGCAGTACCTGCGGGGCATCGTTAAACAGCAGATGTGCCTCGTCGAAGAAAAACACCAGTTTTGGTTTCTCCAGATCGCCCGCTTCCGGCAATTGTTCATACAACTCTGaaa  <  2:864530/140‑1 (MQ=255)                                                                                                                                     |                                                                                                                                         TTTCTGATCTTTTGGCGTAAAAGCCCGCAAAGCATGTTGAACGCGATTACCGAGCTGCCCGAGCACATTATCCGGAATATCAGACGGGTTTTGCGAAACGAACCAGACGCCCACGCCTTTTGAGCGAATAAGCCTTATCACCTGCTCAATCTTATCCAGCAGTACCTGCGGGGCATCGTTAAACAGCAGATGTGCCTCGTCGAAGAAAAACACCAGTTTTGGTTTCTCCAGATCGCCCGCTTCCGGCAATTGTTCATACAACTCTGAAA  >  NC_000913/4489068‑4489336

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑