BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,428,558	T→C	noncoding (205/1542 nt)	rrsD ←	16S ribosomal RNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,428,558	0	T	C	98.8%	442.5 / ‑1.2	163	noncoding (205/1542 nt)	rrsD	16S ribosomal RNA
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (75/86); minor base . (1/1); total (76/87)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.73e-01

Only 100 of 177 total aligned reads displayed.

GGAGTCTGGACCGTGTCTCAGTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAGGGTCCCCCTCTTTGGTCTTGCGACGTTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGTTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAACAGCAAGCTGTTTCCTGTTA  >  NC_000913/3428421‑3428693

ggAGTCTGGACCGTGTCTCAGTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAGGGccc                                                                                                                                       <  1:2398475/140‑1 (MQ=17)

ggAGTCTGGACCGTGTCTCAGTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAGGGccc                                                                                                                                       <  1:1821207/140‑1 (MQ=17)

 gAGTCTGGACCGTGTCTCAGTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAGGGcccc                                                                                                                                      <  1:2401707/140‑1 (MQ=17)

  aGTCTGGACCGTGTCTCAGTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAGGGCcccc                                                                                                                                     <  1:863396/140‑1 (MQ=17)

   gTCTGGACCGTGTCTCAGTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAGGGcccccc                                                                                                                                    >  2:834433/1‑140 (MQ=17)

    tCTGGACCGTGTCTCAGTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAGGGccc                                                                                                                                       <  1:1201111/136‑1 (MQ=17)

    tCTGGACCGTGTCTCAGTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAGGGccc                                                                                                                                       >  2:1201111/1‑136 (MQ=17)

    tCTGGACCGTGTCTCAGTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAGGGCCCCCct                                                                                                                                   >  1:74666/1‑140 (MQ=17)

      tGGACCGTGTCTCAGTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAGGGCCCCCctct                                                                                                                                 <  1:1244552/140‑1 (MQ=17)

           cGTGTCTCAGTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAGGGcccc                                                                                                                                      <  1:2348554/130‑1 (MQ=17)

           cGTGTCTCAGTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAGGGcccc                                                                                                                                      >  2:2348554/1‑130 (MQ=17)

            gtgtCTCAGTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAGGGccc                                                                                                                                       >  2:1142565/1‑128 (MQ=17)

             tgtCTCAGTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAGGGCCCCCctct                                                                                                                                 >  1:2289018/1‑133 (MQ=17)

             tgtCTCAGTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAGGGCCCCCCTCTTTGGTCt                                                                                                                          >  1:1980368/1‑140 (MQ=17)

               tctcAGTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAGGGCCCCCCTCTTTGGTCTTg                                                                                                                        <  1:2136749/140‑1 (MQ=17)

               tctcAGTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAGGGCCCCCCTCTTTGGTCTTg                                                                                                                        <  2:224323/140‑1 (MQ=17)

               tctcAGTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAGGGCCCCCCTCTTTGGTCTTg                                                                                                                        <  2:1980368/140‑1 (MQ=17)

                    gTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAGGGCCCCCCTCTTTGGTCTTGCGACg                                                                                                                   <  2:1511479/140‑1 (MQ=18)

                    gTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCAGGGCACATCCGATGGCAAGAGGCCCGAGGGCCCCCCTCTTTGGTCTTGCGACg                                                                                                                   <  2:2040944/140‑1 (MQ=18)

                     ttCCAGTGTGGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAGGGCCCCCCTCTTTGGTCTTGCGACGt                                                                                                                  <  1:1030332/140‑1 (MQ=18)

                        cAGTGTGGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAGGGCCCCCCTCTTTGGTCTTGCGACGTTAt                                                                                                               <  2:1614130/140‑1 (MQ=25)

                        cAGTGTGGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAGGGCCCCCCTCTTTGGTCTTGCGACGTTAt                                                                                                               <  1:1652133/140‑1 (MQ=25)

                         aGTGTGGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAGGGCCCCCCTCTTTGGTCTTGCGACGTTATg                                                                                                              <  1:598662/140‑1 (MQ=25)

                         aGTGTGGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAGGGCCCCCCTCTTTGGTCTTGCGACGTTATg                                                                                                              <  1:1293458/140‑1 (MQ=25)

                         aGTGTGGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAGGGCCCCCCTCTTTGGTCTTGCGACGTTATg                                                                                                              <  1:1767258/140‑1 (MQ=25)

                         aGTGTGGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAGGGCCCCCCTCTTTGGTCTTGCGACGTTATg                                                                                                              <  2:493058/140‑1 (MQ=25)

                         aGTGTGGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAGGGCCCCCCTCTTTGGTCTTGCGACGTTATg                                                                                                              <  2:754755/140‑1 (MQ=25)

                         aGTGTGGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAGGGCCCCCCTCTTTGGTCTTGCGACGTTATg                                                                                                              <  1:1105185/140‑1 (MQ=25)

                          gtgtGGCTGGTCATCCTCTCAGACCAGTTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAGGGCCCCCCTCTTTGGTCTTGCGACGTTATGc                                                                                                             <  1:1089218/140‑1 (MQ=21)

                          gtgtGGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAGGGCCCCCCTCTTTGGTCTTGCGACGTTATg                                                                                                              >  2:185548/1‑139 (MQ=21)

                          gtgtGGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAGGGCCCCCCTCTTTGGTCTTGCGACGTTATg                                                                                                              <  1:185548/139‑1 (MQ=25)

                          gtgtGGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAGGGCCCCCCTCTTTGGTCTTGCGACGTTATGc                                                                                                             >  2:2150813/1‑140 (MQ=21)

                            gtgGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAGGGCCCCCCTCTTTGGTCTTGCGACGTTATGCgg                                                                                                           <  2:65258/140‑1 (MQ=25)

                             tgGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAGGGCCCCCCTCTTTGGTCTTGCGACGTTATg                                                                                                              >  2:2425277/1‑136 (MQ=21)

                             tgGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAGGGCCCCCCTCTTTGGTCTTGCGACGTTATg                                                                                                              <  1:2425277/136‑1 (MQ=25)

                               gCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAGGGCCCCCCTCTTTGGTCTTGCGACGTTATg                                                                                                              >  2:103201/1‑134 (MQ=21)

                               gCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAGGGCCCCCCTCTTTGGTCTTGCGACGTTATg                                                                                                              <  1:103201/134‑1 (MQ=25)

                               gCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAGGGCCCCCCTCTTTGGTCTTGCGACGTTATGCGGTAt                                                                                                        >  1:802265/1‑140 (MQ=21)

                               gCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAGGGCCCCCCTCTTTGGTCTTGCGACGTTATGCGGTAt                                                                                                        >  2:1014949/1‑140 (MQ=21)

                               gCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAGGG‑CCCCCTCTTTGGTCTTGCGACGTTATGCGGTAtt                                                                                                       >  1:631634/1‑140 (MQ=21)

                                cTGGTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAGGGCCCCCCTCTTTGGTCTTGCGACGTTATGCGGTAtt                                                                                                       >  1:411091/1‑140 (MQ=21)

                                    tCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAGGGCCCCCCTCTTTGGTCTTGCGACGTTATGCGGTATTAGCt                                                                                                   >  1:637845/1‑140 (MQ=21)

                                     cATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGGTGGCAAGAGGCCCGAGGGCCCCCCTCTTTGGTCTTGCGACGTTATGCGGTATTAGCTa                                                                                                  >  2:174213/1‑140 (MQ=21)

                                       tCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAGGGCCCCCCTCTTTGGTCTTGCGACGTTATGCGGTATTAGCTAcc                                                                                                <  1:467296/140‑1 (MQ=25)

                                       tCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAGGGCCCCCCTCTTTGGTCTTGCGACGTTATGCGGTATTAGCTAcc                                                                                                >  1:1145271/1‑140 (MQ=21)

                                        ccTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAGGGCCCCCCTCTTTGGTCTTGCGACGTTATGCGGTATTAGCTACCg                                                                                               >  1:1056060/1‑140 (MQ=21)

                                             cAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAGGGCCCCCCTCTTTGGTCTTGCGACGTTATGCGGTATTAGCTa                                                                                                  >  1:1417383/1‑132 (MQ=21)

                                             cAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAGGGCCCCCCTCTTTGGTCTTGCGACGTTATGCGGTATTAGCTACCGTTTcc                                                                                          <  2:2040811/140‑1 (MQ=21)

                                              aGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAGGGCCCCCCTCTTTGGTCTTGCGACGTTATGCGGTATTAGCTACCGTTTcc                                                                                          <  1:1863465/139‑1 (MQ=25)

                                               gACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAGGGCCCCCCTCTTTGGTCTTGCGACGTTATGCGGTATTAGCTACCGTTTCCag                                                                                        <  1:1014949/140‑1 (MQ=25)

                                                aCCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAGGGCCCCCCTCTTTGGTCTTGCGACGTTATGCGGTATTAGCTACCGTTTc                                                                                           >  2:527216/1‑136 (MQ=21)

                                                    gCTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAGGGCCCCCCTC‑TTGGTCTTGCGACGTTATGCGGTATTAGCTACCGTTTCCAGTAGTTa                                                                                  >  2:540698/1‑140 (MQ=255)

                                                     cTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAGGGCCCCCCTCTTTGGTCTTGCGACGTTATGCGGTATTAGCTAcc                                                                                                >  1:508585/1‑126 (MQ=25)

                                                     cTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAGGGCCCCCCTCTTTGGTCTTGCGACGTTATGCGGTATTAGCTACCGTTTCCAGTAGTTa                                                                                  <  1:2416022/140‑1 (MQ=25)

                                                          gATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAGGGCCCCCCTCTTTGGTCTTGCGACGTTATGCGGTATTAGCTACCGTTTCCAGTAGTTATcccc                                                                             <  1:2384129/140‑1 (MQ=21)

                                                          gATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAGGGCCCCCCTCTTTGGTCTTGCGACGTTATGCGGTATTAGCTACCGTTTCCAGTAGTTATcccc                                                                             <  2:207764/140‑1 (MQ=21)

                                                              gtcgCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAGGGCCCCCCTCTTTGGTCTTGCGACGTTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTcc                                                                         >  2:2114941/1‑140 (MQ=21)

                                                                  ccTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAGGGCCCCCCTCTTTGGTCTTGCGACGTTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCa                                                                     <  1:1498320/140‑1 (MQ=21)

                                                                     tGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAGGGCCCCCCTC‑TTGGTCTTGCGACGTTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCa                                                                 <  1:540698/140‑1 (MQ=255)

                                                                      ggTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAGGGCCCCCCTCTTTGGTCTTGCGACGTTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCt                                                                           <  2:2399055/130‑1 (MQ=25)

                                                                            ccGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAGGGCCCCCCTCTTTGGTCTTGCGACGTTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGTTTcc                                                           <  1:1172666/140‑1 (MQ=37)

                                                                               ttACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAGGGCCCCCCTCTTTGGTCTTGCGACGTTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAg                                                                >  2:940616/1‑132 (MQ=21)

                                                                               ttACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAGGGCCCCCCTCTTTGGTCTTGCGACGTTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAg                                                                <  1:940616/132‑1 (MQ=21)

                                                                               ttACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAGGGCCCCCCTCTTTGGTCTTGCGACGTTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGTTTCCCAg                                                        >  1:2190207/1‑140 (MQ=37)

                                                                               ttACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAGGGCCCCCCTCTTTGGTCTTGCGACGTTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGTTTCCCAg                                                        >  1:1682994/1‑140 (MQ=37)

                                                                                tACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAGGGCCCCCCTCTTTGGTCTTGCGACGTTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGTTTCCCAGa                                                       >  1:1072599/1‑140 (MQ=37)

                                                                                tACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAGGGCCCCCCTCTTTGGTCTTGCGACGTTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGTTTCCCAGa                                                       <  2:1639866/140‑1 (MQ=37)

                                                                                 aCCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAGGGCCCCCCTCTTTGGTCTTGCGACGTTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGTTTCCCAGa                                                       <  1:1326405/139‑1 (MQ=37)

                                                                                    ccaccaACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAGGGCCCCCCTCTTTGGTCTTGCGACGTTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGTTTCCCAGa                                                       >  2:1249279/1‑136 (MQ=37)

                                                                                     caccaACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAGGGCCCCCCTCTTTGGTCTTGCGACGTTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGTTTCCCAGACATTa                                                  >  2:1886057/1‑140 (MQ=37)

                                                                                          acaaGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAGGGCCCCCCTCTTTGGTCTTGCGACGTTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGTTTCCCAGACATTACTCAc                                             >  2:634528/1‑140 (MQ=21)

                                                                                          acaaGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAGGGCCCCCCTCTTTGGTCTTGCGACGTTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGTTTCCCAGACATTACTCAc                                             >  2:466705/1‑140 (MQ=21)

                                                                                               cTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAGGGCCCCCCTCTTTGGTCTTGCGACGTTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGTTTCCCAGACATTACTCACCCGTc                                        >  2:1854986/1‑140 (MQ=18)

                                                                                               cTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAGGGCCCCCCTCTTTGGTCTTGCGACGTTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGTTTCCCAGACATTACTCACCCGTc                                        >  1:1004648/1‑140 (MQ=18)

                                                                                                  aTCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAGGGCCCCCCTCTTTGGTCTTGCGACGTTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGTTTCCCAGAc                                                      <  2:2458107/123‑1 (MQ=17)

                                                                                                   tCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAGGGCCCCCCTCTTTGGTCTTGCGACGTTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGTTTCCCAGACATTACTCACCCGTCCGcc                                    >  2:2344568/1‑140 (MQ=18)

                                                                                                    cccATCTGGGCACATCCGATGGCAAGAGGCCCGAGGGCCCCCCTCTTTGGTCTTGCGACGTTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGTTTCCCAGACATTACTCACCCGTCCGCCa                                   <  1:2105125/140‑1 (MQ=17)

                                                                                                     ccATCTGGGCACATCCGATGGCAAGAGGCCCGAGGGCCCCCCTCTTTGGTCTTGCGACGTTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGTTTCCCAGACATTACTCACCCGTCCGCCAc                                  <  2:243783/140‑1 (MQ=17)

                                                                                                        tCTGGGCACATCCGATGGCAAGAGGCCCGAGGGCCCCCCTCTTTGGTCTTGCGACGTTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGTTTCCCAGACATTACTCACCCGTCCGCCACTCg                               >  1:2050108/1‑140 (MQ=18)

                                                                                                        tCTGGGCACATCCGATGGCAAGAGGCCCGAGGGCCCCCCTCTTTGGTCTTGCGACGTTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGTTTCCCAGACATTACTCACCCGTCCGCCACTCg                               >  1:679028/1‑140 (MQ=18)

                                                                                                        tCTGGGCACATCCGATGGCAAGAGGCCCGAGGGCCCCCCTCTTTGGTCTTGCGACGTTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGTTTCCCAGACATTACTCACCCGTCCGCCACTCg                               >  1:1390602/1‑140 (MQ=17)

                                                                                                         cTGGGCACATCCGATGGCAAGAGGCCCGAGGGCCCCCCTCTTTGGTCTTGCGACGTTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGTTTCCCAGACATTACTCACCCGTCCGCCACTCGt                              <  1:1736252/140‑1 (MQ=17)

                                                                                                          tGGGCACATCCGATGGCAAGAGGCCCGAGGGCCCCCCTCTTTGGTCTTGCGACGTTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGTTTCCCAGACATTACTCACCCGTCCGCCACTCGTc                             <  2:2141231/140‑1 (MQ=17)

                                                                                                          tGGGCACATCCGATGGCAAGAGGCCCGAGGGCCCCCCTCTTTGGTCTTGCGACGTTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGTTTCCCAGACATTACTCACCCGTCCGCCACTCGTc                             <  2:2164766/140‑1 (MQ=17)

                                                                                                            ggCACATCCGATGGCAAGAGGCCCGAGGGCCCCCCTCTTTGGTCTTGCGACGTTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGTTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAg                           >  2:1104805/1‑140 (MQ=17)

                                                                                                                caTCCGATGGCAATAGGCCCGAGGGCCCCCCTCTTTGGTCTTGCGACGTTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGTTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGaa                       <  1:2296270/140‑1 (MQ=21)

                                                                                                                caTCCGATGGCAAGAGGCCCGAGGGCCCCCCTCTTTGGTCTTGCGACGTTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGTTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGaa                       <  2:1072599/140‑1 (MQ=21)

                                                                                                                 aTCCGATGGCAAGAGGCCCGAGGGCCCCCCTCTTTGGTCTTGCGACGTTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGTTTCCCAGACATTACTCTCCCGTCCGCCACTCGTCAGCGaaa                      <  1:1628775/140‑1 (MQ=21)

                                                                                                                    cGATGGCAAGAGGCCCGAGGGCCCCCCTCTTTGGTCTTGCGACGTTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGTTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAACAg                   >  2:721969/1‑140 (MQ=18)

                                                                                                                     gATGGCAAGAGGCCCGAGGGCCCCCCTCTTTGGTCTTGCGACGTTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGTTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAACAGc                  <  2:1390602/140‑1 (MQ=17)

                                                                                                                      aTGGCAAGAGGCCCGAGGGCCCCCCTCTTTGGTCTTGCGACGTTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGTTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAACAGCa                 >  1:125441/1‑140 (MQ=18)

                                                                                                                      aTGGCAAGAGGCCCGAGGGCCCCCCTCTTTGGTCTTGCGACGTTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGTTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAACAGCa                 >  1:470685/1‑140 (MQ=18)

                                                                                                                           aaGAGGCCCGAGGGCCCCCCTCTTTGGTCTTGCGACGTTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGTTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAACAGCAAGCTg            <  1:634528/140‑1 (MQ=17)

                                                                                                                           aaGAGGCCCGAGGGCCCCCCTCTTTGGTCTTGCGACGTTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGTTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAACAGCAAGCTg            >  2:1022672/1‑140 (MQ=17)

                                                                                                                           aaGAGGCCCGAGGGCCCCCCTCTTTGGTCTTGCGACGTTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGTTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAACAGCAAGCTg            >  1:1363215/1‑140 (MQ=18)

                                                                                                                             gagGCCCGAGGGCCCCCCTCTTTGGTCTTGCGACGTTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGTTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAACAGCAAGCTGtt          <  1:2150813/140‑1 (MQ=17)

                                                                                                                             gagGCCCGAGGGCCCCCCTCTTTGGTCTTGCGACGTTATGCGGGATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGTTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAACAGCAAGCTGtt          >  2:2272222/1‑140 (MQ=17)

                                                                                                                             gagGCCCGAGGGCCCCCCTCCTTGGTCTTGCGACGTTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGTTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAACAGCAAGCTGtt          <  2:360500/140‑1 (MQ=17)

                                                                                                                              agGCCCGAGGG‑CCCCCTCTTTGGTCTTGCGACGTTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGTTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAACAGCAAGCTGTTTc        <  2:631634/140‑1 (MQ=17)

                                                                                                                                     aGGGCCCCCCTCTTTGGTCTTGCGACGTTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGTTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAACAGCAAGCTGTTTCCTGTTa  >  1:1222539/1‑140 (MQ=11)

GGAGTCTGGACCGTGTCTCAGTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAGGGTCCCCCTCTTTGGTCTTGCGACGTTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGTTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAACAGCAAGCTGTTTCCTGTTA  >  NC_000913/3428421‑3428693

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`