| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 445,558 | 0 | A | C | 30.6% | 70.0 / 11.5 | 36 | V370G (GTA→GGA) | yajR | putative major facilitator superfamily transport protein YajR |
| Reads supporting (aligned to +/- strand): ref base A (20/5); new base C (0/11); total (20/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.27e-06 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
AACGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGC‑GAGAAATACCCCCTGACCGT‑CAAACATGCCGTTAATCCAG‑CCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTACTGATAAGTG > NC_000913/445424‑445693 | aaCGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGC‑GAGAAATAccccc < 1:1243066/140‑1 (MQ=255) aCGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTTTTTCATGGGACTGGCGACCGTCAACCACACTGCGGCCCGCATTTCGCCAGC‑GGGAAAAAcccccc > 2:147310/1‑139 (MQ=255) aCGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGGCTGTCAGCCCCACCGCGGGCCGCCTTTCGCCCGCGGGGAAAAAccccc > 2:30080/1‑140 (MQ=255) aCGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGC‑GAGAAATACCCCCt < 1:140739/140‑1 (MQ=255) aCGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGC‑GAGAAATACCCCCt < 2:1039347/140‑1 (MQ=255) aCGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGGTCTTTCATGGTACTTGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGG‑GAGAAATAcccccc > 2:661862/1‑139 (MQ=255) gCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGC‑GAGAAATACCCCCTGACCGT‑CAAACAt < 1:852007/140‑1 (MQ=255) tCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTTATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTTCGCCAGC‑GAGAAAAACCCCCCGACCGT‑CAAACATGcc > 2:335243/1‑140 (MQ=255) cAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGC‑GAGAAATACCCCCTGACCGT‑CAAACATGCCGTTAATCCAg < 2:1068074/140‑1 (MQ=255) aaaTTTTCCCCGGGATTTCAATGCGTAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGC‑GAGAAATCCCCCCTGACCGT‑CAAACATGCCGTTAATCCAg < 2:553972/139‑1 (MQ=255) aTGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCCGCATTTCGCCCGC‑GAGAAAAACCCCCCGACCGT‑CAAACAAGCCGTTAATCCAg‑cc > 2:996971/1‑140 (MQ=255) gATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGC‑GAAAAATCCCCCCTGACCGT‑CAAACATGCCGTTAATCCAG‑CCGCCCAGCGAAc < 2:1246182/140‑1 (MQ=255) cgcAAACTGCTGACATACGGCGGTTCTTTCATGGGACTGGCGACCGTCAGCCACACTGCGGCCAGCATTGCGCCAGC‑GAGAAAAAACCCCCGACCGT‑CAAACATGCCGTTAATCCCG‑CCCCCCCGCGAACCGCCAATcgc > 2:1210019/1‑140 (MQ=255) tgACATACGGCGGTTCTTTTCTGGGACGTGGGACTGGGAAGCACACCGTGGCCCGCACTGCTGCCGC‑GGGAAATACCCCCTGTCCAC‑GAAACATCACGTTAATCCAG‑CCTCCCCGCGAACCCCCCATCGCCCCTCCCAGa > 2:256636/1‑140 (MQ=255) tgACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCCGC‑GAGAAAAACCCCCCGACCGT‑CAAACATGCCGTTAATCCAG‑CCCCCCAGCGAACCGCCAAACGCCCCCCCAAGa > 2:353885/1‑140 (MQ=255) gACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTTCGCCAGC‑GAGAAATACCCCCTGACCGT‑CAAACATGCCCGTAATCCAG‑CCGCCCCGCGAACCGCCAATCGCCACGCCAAGaa > 1:730740/1‑140 (MQ=255) aCATACGGCGGTTTTTTCAGGGTACGGGCGCCTGTCACCCACACTGCGGCCAGCATTGCGCCAGA‑GAGAAATCCCCCCTGACCGT‑CAAACATGCCGTTAATCCAG‑CCGCCCAGCGAACCGCCAATCGCCACGCCAAGaaa < 2:731168/140‑1 (MQ=255) gTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGC‑GAGAAATCCCCCCTGACCGT‑CAAACATGCCGTTAATCCAG‑CCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACAcc < 1:798966/140‑1 (MQ=255) gTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGC‑GAGAAATACCCCCTGACCGT‑CAAACATGCCGTTAATCCAG‑CCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACAcc > 1:577747/1‑140 (MQ=255) gTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGC‑GAGAAATACCCCCTGACCGT‑CAAACATGCCGTTAATCCAG‑CCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACAcc > 2:47962/1‑140 (MQ=255) cTGGCGACTGTCAGCCACACTGCGGCAAGCATTGCGCCAGC‑GAGAAATCCCCCCTGACCGT‑CAAACATGCCGTTAATCCAG‑CCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCAt < 1:27310/140‑1 (MQ=255) tGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGC‑GAGAAATACCCCCTGACCGT‑CAAACATGCCGTTAATCCAG‑CCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATc > 1:345591/1‑140 (MQ=255) ggCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGC‑GAGAAATACCCCCTGACCGT‑CAAACATGCCGTTAATCCAG‑CCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCg > 2:426339/1‑140 (MQ=255) ggCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGC‑GAGAAATACCCCCTGACCGT‑CAAACATGCCGTTAATCCAG‑CCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCg > 2:1097671/1‑140 (MQ=255) gtgACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGC‑GAGAAATCCCCCCTGCCCGT‑CAAACATGCCGTTAATCCAG‑CCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGc < 1:66115/138‑1 (MQ=255) gCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGC‑GAGAAATACCCCCTGACCGT‑CAAACATGCCGTTAATCCAG‑CCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGc > 2:1036260/1‑140 (MQ=255) gCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGC‑GAGAAATACCCCCTGACCGT‑CAAACATGCCGTTAATCCAG‑CCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGc > 2:109948/1‑140 (MQ=255) gCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGC‑GAGAAATACCCCCTGACCGT‑CAAACATGCCGTTAATCCAG‑CCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGc > 2:180942/1‑140 (MQ=255) gccTGCCAGCCACACTGCGGCCAGCATTGCGCCAGC‑GAAAAATCCCCCCTGCCCGC‑CAAACATGCCGTTAATCCAG‑CCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCg < 2:199863/138‑1 (MQ=255) cTGTCAGCCACCCGGCGGCCAGCATGGCGCCAGC‑GAAAAATCCCCCCTGCCCGT‑AAAACATGCCGTTAATCCAG‑CCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTa < 2:577747/140‑1 (MQ=255) cTGTCACCCACACGGCGGCCAGCATGGCGCCAGC‑GAAAAATCCCCCCGGCCCGT‑CAACCATGCCGTTAATCCAG‑CCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTa < 2:726419/140‑1 (MQ=255) tGTCAGCCACACTGCGGCCAGCATTGCGCCAGC‑GAGAAATACCCCCTGACCGT‑CAAACATGCCGTTAATCCAG‑CCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTAc > 1:31436/1‑140 (MQ=255) tGTCAGCCACACTGCGGCCAGCATTGCGCCAGC‑GAGAAATACCCCCTGACCGT‑CAAACATGCCGTTAATCCAG‑CCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTAc > 1:619317/1‑140 (MQ=255) tGTCAGCCACACTGCGGCCAGCATTGCGCCAGC‑GAGAAATACCCCCTGACCGT‑CAAACATGCCGTTAATCCAG‑CCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTAc > 2:94972/1‑140 (MQ=255) cacaCTGCGGCCAGCATTGCGCCAGC‑GAGAAATCCCCCCTGACCGT‑CAAACATGCCGTTAATCCAG‑CCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTa < 1:180942/140‑1 (MQ=255) acTGCGGCCAGCATTGCGCCAGC‑GAGAAATACCCCCTGACCGT‑CAAACATGCCGTTAATCCAG‑CCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAAcc > 1:770250/1‑140 (MQ=255) cTGCGGCCAGCATGGCGCCAGC‑GAGAAATCCCCCCTGCCCGT‑CAAACATGCCGTTAATCCAG‑CCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACct < 1:661862/140‑1 (MQ=255) tGCGGCCAGCATTGCGCCAGC‑GAGAAATACCCCCTGACCGT‑CAAACATGCCGTTAATCCAG‑CCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACctg > 1:1166595/1‑140 (MQ=255) gCGGCCAGCATGGCCCCAGC‑GAAAAATCCCCCCTGACCGT‑CAAACATGCCGTTAATCCAG‑CCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACctgc < 2:696557/140‑1 (MQ=255) cGGCCAGCATTGCGCCAGC‑GAGAAATACCCCCTGACCGT‑CAAACATGCCGTTAATCCAG‑CCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACctgct > 2:523951/1‑140 (MQ=255) gCCAGCATTGCGCCAGC‑GAGAAATACCCCCTGACCGT‑CAAACATGCCGTTAATCCAG‑CCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTgg > 1:454615/1‑140 (MQ=255) agcttggcgcAAGC‑GAAAAATCCCCCCTGACCGT‑AAAACATGCCGTTAATCCAG‑CCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGa < 1:1278217/134‑1 (MQ=255) acaaTTCCGCCAGC‑AAGAAATCCCCCCGGACCGT‑AAAACACGCCGTTAACCCAC‑CCGCCCAGCAAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGa < 1:256636/137‑1 (MQ=255) aGCATTCCGCC‑GC‑CAAAAATCCCCCCTGGCCCTAAAAACATG‑CGTTAAACCCGCCCCCCCACCAAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGa < 2:639348/140‑1 (MQ=255) gctttGCGCCAGC‑GAGAAATCCCCCCTGCCCGT‑CAAACATGCCGTTAATCCAG‑CCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGAc < 1:336261/137‑1 (MQ=255) cATTGCGCCAGC‑GAGAAATCCCCCCTGACCGT‑CAAACATGCCGTTAATCCAG‑CCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGAct < 1:996971/140‑1 (MQ=255) ttccgcCAGC‑GAAAAATCCCCCCTGACCGT‑CAAACATGCCGTTAATCCAG‑CCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGActct < 2:454615/137‑1 (MQ=255) gcgcCAGC‑GAGAAATCCCCCCTGCCCGT‑CAAACATGCCGTTAATCCAG‑CCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCttt < 2:213895/140‑1 (MQ=255) gcgcCAGC‑GAAAAATCCCCCCTGACCGT‑CAAACATGCCGTTAATCCAG‑CCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAACCACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCttt < 1:353885/140‑1 (MQ=255) cgcCAGC‑GAGAAATACCCCCTGACCGT‑CAAACATGCCGTTAATCCAG‑CCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTa > 2:1009189/1‑140 (MQ=255) aGC‑GAGAAATCCCCCGTGACCGC‑CAAACATGCCGTTAATCCAG‑CCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTACTGa < 2:105368/140‑1 (MQ=255) gC‑GAGAAATACCCCCTGACCGT‑CAAACATGCCGTTAATCCAG‑CCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTACTGAt > 1:121370/1‑140 (MQ=255) gC‑GAGAAATACCCCCTGACCGT‑CAAACATGCCGTTAATCCAG‑CCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGGAACCTGCTGGCGACTCTTTACTGAt > 1:1105618/1‑140 (MQ=255) aaaaTCCCCCCTGACCGT‑CAAACATGCCGTTAATCCAG‑CCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTACTGATAAGt < 1:47962/139‑1 (MQ=255) aaaTACCCCCTGACCGT‑CAAACATGCCGTTAATCCAG‑CCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTACTGATAAGTg > 2:189643/1‑140 (MQ=255) | AACGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGC‑GAGAAATACCCCCTGACCGT‑CAAACATGCCGTTAATCCAG‑CCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTACTGATAAGTG > NC_000913/445424‑445693 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |