| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,377,140 | 0 | A | C | 34.6% | 92.1 / 13.6 | 52 | K103N (AAA→AAC) | ycjS | putative oxidoreductase YcjS |
| Reads supporting (aligned to +/- strand): ref base A (27/7); new base C (0/18); total (27/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.13e-08 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
ATGCATCCGTGTGGGACGATCCGCAGGCCATGCTGCTGGCGGTGAAACCTGATGTGGTTAGCGTCTGCTCACCTAACCGTTTTCATTACGAACATACCCTGATGGCACTGGAAGCGGGCTGCCATGTGATGTGCGAAAAACCGCCCGCCATGACGCCAGAACAGGCGCGGGAAA‑‑TGTGCGATACCGCGCGCAAACTGGGCAAGGTGCTGGCCTACGACTTTCACCATCGTTTTGCGCTCGATACGCAACAGCTGCGTGAACAGGTGACCAACGGCGTT > NC_000913/1377001‑1377278 | aTGCATCCGTGTGGGACGATCCGCAGGCCATGCTGCTGGCGGTGAAACCTGATGTGGTTAGCGTCTGCTCACCTAACCGTTTTCATTACGAACATACCCTGATGGCACTGGAAGCGGGCTGCCATGTGATGTGCGaaaaa > 1:190810/1‑140 (MQ=255) gACGATCCGCAGGCCATGCTGCTGGCGGTGAAACCTGATGTGGTTAGCGTCTGCTCACCTAGCCGTTTTCATTACGAACATCCCCTGATGGCACTGGAAGCGGGCTGCCATGTGATGTGCGAAAACCCGCCCGCCATGAc < 1:998798/140‑1 (MQ=255) tCCGCAGGCCATGCTGCTGGCGGTGAAACCTGATGTGGTTAGCGTCTGCTCACCTAACCGTTTTCATTACGAACATACCCTGATGGCACTGGAAGCGGGCTGCCATGTGATGTGCGAAAAACCGCCCGCCATGACGCCAg > 2:976010/1‑140 (MQ=255) aGGCCATGCTGCTGGCGGTGAAACCTGATGTGGTTAGCGTCTGCTCACCTAACCGTTTTCATTACGAACATACCCTGATGGCACTGGAAGCGGGCTGCCATGTGATGTGCGAAAAACCGCCCGCCATGACGCCAGAACAg > 1:311743/1‑140 (MQ=255) tgctgcTGGCGGTGAACCCTGATGTGGTTAGCGTCTGCTCCCCTACCCTTTTTCATTACGAACATCCCCTGATGCCACGGGAGGCGGGCTGCCATGTGATGTGCAAAAACCCGCCCGCCATGACGCCAGAACAGGCGCgg < 2:311743/140‑1 (MQ=255) gctgctGGCGGTAAAACCTGATGTGGTTAGCGTCTGCTCACCTAACCGTTTTCATTACGAACATACCCTGATGGCACTGGAAGCGGGCTGCCATGTGATGTGCAAAAACCCGCCCGCCATGACGCCAGAACAGGCGCggg < 2:232119/140‑1 (MQ=255) tgctgGCGGTGAACCCTGATGTGGTTAGCTTCTCCTCACCTACCCTTTTTCATTACAAACATCCCCTGATGGCACGGAAGGCGGGCTGCCATGTGATGTGCAAAAACCCGCCCGCCATGACGCCAGAACAGGCGCGGGaa < 2:310410/140‑1 (MQ=255) tgctgGCGGTGAACCCTGATGTGGTTAGCGTCTGCTCACCTACCCGTTTTCATTACGAACATCCCCTGATGGCACTGGAAGCGGGCTGCCATGTGATGTGCGAAAACCCGCCCGCCATGACGCCAGAACAGGCGCGGGaa < 1:557867/140‑1 (MQ=255) tgctgGCGGTGAAACCTGATGTGGTTAGCGTCTGCTCACCTAACCGTTTTCATTACGAACATCCCCTGATGGCACTGGAAGCGGGCTGCCATGTGATGTGCGAAAACCCGCCCGCCATGACGCCAGAACAGGCGCGGGaa < 1:628820/140‑1 (MQ=255) tgctgGCGGTGAAACCTGATGTGGTTAGCGTCTGCTCACCTAACCGTTTTCATTACGAACATCCCCTGATGGCACTGGAAGCGGGCTGCCATGTGATGTGCGAAAAACCGCCCGCCATGACGCCAGAACAGGCGCGGGaa < 1:914088/140‑1 (MQ=255) tgctgGCGGTGAAACCTGATGTGGTTAGCGTCTGCTCACCTAACCGTTTTCATTACGAACATACCCTGATGGCACTGGAAGCGGGCTGCCATGTGATGTGCGAAAAACCGCCCGCCATGACGCCAGAACAGGCGCGGGaa < 2:862949/140‑1 (MQ=255) tGAAACCTGATGTGGTTAGCGTCTGCTCACCTAACCGTTTTCATTACGAACATACCCTGATGGCACTGGAAGCGGGCTGCCATGTGATGTGCGAAAAACCGCCCGCCATGACGCCAGAACAGGCGCGGGAAA‑‑TGTGCGAt > 1:266828/1‑140 (MQ=255) tgtgGTTAGCGTCTGCTCACCTAACCGTTTTCATTACGAACATACCCTGATGGCACTGGAAGCGGGCTGCCATGTGATGTGCGAAAAACCGCCCGCCATGACGCCAGAACAGGCGCGGGAAA‑‑TGTGCGATACCGCGCGCa > 1:673603/1‑140 (MQ=255) tAGCGTCTGCTCACCTAACCGTTTTCATTACGAACATACCCTGATGGCACTGGAAGCGGGCTGCCATGTGATGTGCGAAAAACCGCCCGCCATGACGCCAGAACAGGCGCGGGAAA‑‑TGTGCGATACCGCGCGCAAACTgg > 1:117787/1‑140 (MQ=255) cGTCTGCTCACCTAACCGTTTTCATTACGAACATACCCTGATGGCACTGGAAGCGGGCTGCCATGTGATGTGCGAAAAACCGCCCGCCATGACGCCAGAACAGGCGCGGGAAA‑‑TGTGCGATACCGCGCGCAAACTGGGCa > 1:147056/1‑140 (MQ=255) tCTGCTCACCTAACCGTTTTCATTACGAACATCCCCTGATGGCACGGGAAGCGGGCTGCCATGTGATGTGCGAAAACCCGCCCGCCATGACGCCAGAACAGGCGCGGGAAA‑‑TGTGCGATACCGCGCGCAAACTGGGCAAg < 2:605217/140‑1 (MQ=255) gCTCACCTAACCGTTTTCATTACGAACATACCCTGATGGCACTGGAAGCGGGCTGCCATGTGATGTGCGAAAAACCGCCCGCCATGACGCCAGAACAGGCGCGGGAAA‑‑TGTGCGATACCGCGCGCAAACTGGGCAAGGTg > 1:304183/1‑140 (MQ=255) gCTCACCTAACCGTTTTCATTACGAACATACCCTGATGGCACTGGAAGCGGGCTGCCATGTGATGTGCGAAAAACCGCCCGCCATGACGCCAGAACAGGCGCGGGAAA‑‑TGTGCGATACCGCGCGCAAACTGGGCAAGGTg > 2:697218/1‑140 (MQ=255) gCTCACCTAACCGTTTTCATTACGAACATACCCTGATGGCACTGGAAGCGGGCTGCCATGTGATGTGCGAAAAACCGCCCGCCATGACGCCAGAACAGGCGCGGGAAA‑‑TGTGCGATACCGCGCGCAAACTGGGCAAGGTg > 1:228076/1‑140 (MQ=255) cTCACCTAACCGTTTTCATTACGAACATCCCCTGATGGCACTGGAAGCGGGCTGCCATGTGATGTGCGAAAACCCGCCCGCCATGACGCCAGAACAGGCGCGGGAAA‑‑TGTGCGATACCGCGCGCAAACTGGGCAAGGTGc < 1:1153636/140‑1 (MQ=255) cTCACCTAACCGTTTTCATTACGAACATACCCTGATGGCACTGGAAGCGGGCTGCCATGTGATGTGCGAAAAACCGCCCGCCATGACGCCAGAACAGGCGCGGGAAA‑‑TGTGCGATACCGCGCGCAAACTGGGCAAGGTGc > 2:806260/1‑140 (MQ=255) cACCTAACCGTTTTCATTACGAACATCCCCTGAGGGCTGGGGAGGCGGGGTGCCATGTGATGTGAAAAAACCCGCCCGCCATGACGCCAGAACAGGCGCGGGAAA‑‑TGTGCGATACCGCGCGCAAACTGGGCAAGGTGCTg < 2:47490/140‑1 (MQ=255) ccTAACCGTTTTCATTACGAACATACCCTGATGGCACTGGAAGCGGGCTGCCATGTGATGTGCGAAAAACCGCCCGCCATGACGCCAGAACAGGCGCGGGAAA‑‑TGTGCGATACCGCGCGCAAACTGGGCAAGGTGCTGGc > 2:2385/1‑140 (MQ=255) aaCCGTTTTCATTACGAACATACCCTGATGGCACTGGAAGCGGGCTGCCATGTGATGTGCAAAAACCCGCCCGCCATGACGCCAGAACAGGCGCGGGAAA‑‑TGTGCGATACCGCGCGCAAACTGGGCAAGGTGCTGGCCTa < 2:620969/140‑1 (MQ=255) ttttCATTACGAACATCCCCTGATGGCACTGGAAGCGGGCTGCCATGTGATGTGCGAAAAACCGCCCGCCATGACGCCAGAACAGGCGCGGGAAA‑‑TGTGCGATACCGCGCGCAAACTGGGCAAGGTGCTGGCCTACGACt < 1:1230429/140‑1 (MQ=255) tttaTTCCGAACATCCCCTGATGGCATTGGAAGCGGCTTGCTATGTGATGTGCAAAAACCCGCCCGCCATGACGCCAGAACAGGCGCGGGAAA‑‑TGTGCGATACCGCGCGCAAACTGGGCAAGGTGCTGGCCTACGACttt < 2:632585/137‑1 (MQ=255) ttCATTACGAACATCCCCTGATGGCACTGGAAGCGGGCTGCCATGTGATGTGCGAAAACCCGCCCGCCATGACACCAGAACAGGCGCGGGAAA‑‑TGTGCGATACCGCGCGCAAACTGGGCAAGGTGCTGGCCTACGACttt < 1:909965/140‑1 (MQ=255) ttCATTACGAACATACCCTGATGGCACTGGAAGCGGGCTGCCATGTGATGTGCGAAAAACCGCCCGCCATGACGCCAGAACAGGCGCGGGAAA‑‑TGTGCGATACCGCGCGCAAACTGGGCAAGGTGCTGGCCTACGACttt > 2:213302/1‑140 (MQ=255) cATTACGAACATACCCTGATGGCACTGGAAGCGGGCTGCCATGTGATGTGCGAAAAACCGCCCGCCATGACGCCAGAACAGGCGCGGGAAA‑‑TGTGCGATACCGCGCGCAAACTGGGCAAGGTGCTGGCCTACGACTTTCa < 1:976010/140‑1 (MQ=255) gAACATACCCTGATGGCACTGGAAGCGGGCTGCCATGTGATGTGCGAAAAACCGCCCGCCATGACGCCAGAACAGGCGCGGGAAA‑‑TGTGCGATACCGCGCGCAAACTGGGCAAGGTGCTGGCCTACGACTTTCACCATCg > 1:872503/1‑140 (MQ=255) aCCCTGATGGCACTGGAAGCGGGCTGCCATGTGATGTGCGAAAAACCGCCCGCCATGACGCCAGAACAGGCGCGGGAAA‑‑TGTGCGATACCGCGCGCAAACTGGGCAAGGTGCTGGCCTACGACTTTCACCATCGTTTTgc > 1:559220/1‑140 (MQ=255) cccTGATGGCACTGGAAGCGGGCTGCCATGTGATGTGCAAAAACCCGCCCGCCATGACGCCAGAACAGGCGCGGGAAA‑‑TGTGCGATACCGCGCGCAAACTGGGCAAGGTGCTGGCCTACGACTTTCACCATCGTTTTgcg < 2:266828/140‑1 (MQ=255) aTGGCACTGGAAGCGGGCTGCCATGTGATGTGCGAAAACCCGCCCGCCATGACGCCAGAACAGGCGCGGGAAA‑‑TGTGCGATACCGCGCGCAAACTGGGCAAGGTGCTGGCCTACGACTTTCACCATCGTTTTGCGCt < 2:1255888/137‑1 (MQ=255) aTGGCACTGGAAGCGGGCTGCCATGTGATGTGCGAAAAACCGCCCGCCATGACGCCAGAACAGGCGCGGGAAA‑‑TGTGCGATACCGCGCGCAAACTGGGCAAGGTGCTGGCCTACGACTTTCACCATCGTTTTGCGCt > 1:1255888/1‑137 (MQ=255) aTGGCACTGGAAGCGGGCTGCCATGTGATGTGCGAAAAACCGCCCGCCATGACGCCAGAACAGGCGCGGGAAA‑‑TGTGCGATACCGCGCGCAAACTGGGCAAGGTGCTGGCCTACGACTTTCACCATCGTTTTGCGCTCGa > 2:1010603/1‑140 (MQ=255) tGGCACTGGAAGCGGGCTGCCATGTGATGCGCGAAAAACCGCCCGCCATGACGCCAGAACAGGCGCGGGAAATGTGTGCGATACCGCGCGCAAACTGGGCAAGGGGCTGGCCTACGACTTTCACCATCGTTTTGCGCTCg > 2:1045265/1‑140 (MQ=255) ggCACTGGAAGCGGCCTGCCATGTGATGTGCAAAAACCCGCCCGCCATGACGCCAGAACAGGCGCGGGAAA‑‑TGTGCGATACCGCGCGCAAACTGGGCAAGGTGCTGGCCTACGACTTTCACCATCGTTTTGCGCTCGATa < 2:190810/140‑1 (MQ=255) cACTGGAAGCGGGCTGCCATGTGATGTGCGAAAAACCGCCCGCCATGACGCCAGAACAGGCGCGGGAAA‑‑TGTGCGATACCGCGCGCAAACTGGGCAAGGTGCTGGCCTACGACTTTCACCATCGTTTTGCGCTCGATACg > 2:1181202/1‑140 (MQ=255) cACTGGAAGCGGGCTGCCATGTGATGTGCGAAAAACCGCCCGCCATGACGCCAGAACAGGCGCGGGAAA‑‑TGTGCGATACCGCGCGCAAACTGGGCAAGGTGCTGGCCTACGACTTTCACCATCGTTTTGCGCTCGATACg > 1:325072/1‑140 (MQ=255) aCTGGAAGCGGGCTGCCATGTGATGTGCGAAAAACCGCCCGCCATGACGCCAGAACAGGCGCGGGAAA‑‑TGTGCGATACCGCGCGCAAACTGGGCAAGGTGCTGGCCTACGACTTTCACCATCGTTTTGCGCTCGATACGc > 1:767115/1‑140 (MQ=255) gAAGCGGGCTGCCATTTGTTGTGCGAAAACCCGCCCGCCATGACGCCAGAACAGGCGCGGGAAA‑‑TGTGCGATACCGCGCGCAAACTGGGCAAGGTGCTGGCCTACGACTTTCACCATCGTTTTGCGCTCGATACGCAACa < 1:876674/140‑1 (MQ=255) gAAGCGGGCTGCCATGTGATGTGCGAAAACCCGCCCGCCATGACGCCAGAACAGGCGCGGGAAA‑‑TGTGCGATACCGCGCGCAAACTGGGCAGGGTGCTGGCCTACGACTTTCACCATCGTTTTGCGCTCGATACGCAACa < 1:540041/140‑1 (MQ=255) gAAGCGGGCTGCCATGTGATGTGCGAAAACCCGCCCGCCATGACGCCAGAACAGGCGCGGGAAA‑‑TGTGCGATACCGCGCGCAAACTGGGCAAGGTGCTGGCCTACGACTTTCACCATCGTTTTGCGCTCGATACGCAACa < 2:837704/140‑1 (MQ=255) gCGGGCTGCCATGTGATGTGCGAAAAACCGCCCGCCATGACGCCAGAACAGGCGCGGGAAA‑‑TGTGCGATACCGCGCGCAAACTGGGCAAGGTGCTGGCCTACGACTTTCACCATCGTTTTGCGCTCGATACGCAACAGCt < 1:806260/140‑1 (MQ=255) cGGGCTGCCATGTGATGTGCGAAAAACCGCCCGCCATGACGCCAGAACAGGCGCGGGAAA‑‑TGTGCGATACCGCGCGCAAACTGGGCAAGGTGCTGGCCTACGACTTTCACCATCGTTTTGCGCTCGATACGCAACAGCTg > 2:35907/1‑140 (MQ=255) gCCTTGTGATGTGCAAAAACCCGCCCGCCATGACGCCAGAACAGGCGCGGGAAA‑‑TGTGCGATACCGCGCGCAAACTGGGCAAGGTGCTGGCCTACGACTTTCACCATCGTTTTGCGCTCGATACGCAACAGCTGCGTGaa < 2:325072/140‑1 (MQ=255) gCCATGTGATGTGCGAAAAACCGCCCGCCATGACGCCAGAACAGGCGCGGGAAA‑‑TGTGCGATACCGCGCGCAAACTGGGCAAGGTGCTGGCCTACGACTTTCACCATCGTTTTGCGCTCGATACGCAACAGCTGCGTGaa < 1:2385/140‑1 (MQ=255) gCCATGTGATGTGCGAAAAACCGCCCGCCATGACGCCAGAACAGGCGCGGGAAA‑‑TGTGCGATACCGCGCGCAAACTGGGCAAGGTGCTGGCCTACGACTTTCACCATCGTTTTGCGCTCGATACGCAACAGCTGCGTGaa > 2:95142/1‑140 (MQ=255) gCCATGTGATGTGCGAAAAACCGCCCGCCATGACGCCAGAACAGGCGCGGGAAA‑‑TGTGCGATACCGCGCGCAAACTGGGCAAGGTGCTGGCCTACGACTTTCACCATCGTTTTGCGCTCGATACGCAACAGCTGCGTGaa > 2:444430/1‑140 (MQ=255) gCCATGTGATGTGCAAAAACCCGCCCGCCATGACGCCAGAACAGGCACGGGAAA‑‑TGTGCGATACCGCGCGCAAACTGGGCAAGGTGCTGGCCTACGACTTTCACCATCGTTTTGCGCTCGATACGCAACAGCTGCGTGaa < 2:1135528/140‑1 (MQ=255) ttgtgatgtgCGAAAACCCGCCCGCCATGACGCCAGAACAGGCGCGGGAAA‑‑TGTGCGATACCGCGCGCAAACTGGGCAAGGTGCTGGCCTACGACTTTCACCATCGTTTTGCGCTCGATACGCAACAGCTGCGTGAACAg < 2:564440/139‑1 (MQ=255) tgCGAAAAACCGCCCGCCATGACGCCAGAACAGGCGCGGGAAA‑‑TGTGCGATACCGCGCGCAAACTGGGCAAGGTGCTGGCCTACGACTTTCACCATCGTTTTGCGCTCGATACGCAACAGCTGCGTGAACAGGTGACCaa > 2:161879/1‑140 (MQ=255) tgCGAAAAACCGCCCGCCATGACGCCAGAACAGGCGCGGGAAA‑‑TGTGCGATACCGCGCGCAAACTGGGCAAGGTGCTGGCCTACGACTTTCACCATCGTTTTGCGCTCGATACGCAACAGCTGCGTGAACAGGTGACCaa > 1:123942/1‑140 (MQ=255) tgCGAAAAACCGCCCGCCATGACGCCAGAACAGGCGCGGGAAA‑‑TGTGCGATACCGCGCGCAAACTGGGCAAGGTGCTGGCCTACGACTTTCACCATCGTTTTGCGCTCGATACGCAACAGCTGCGTGAACAGGTGACCaa > 2:194832/1‑140 (MQ=255) gggaaaaatCCGCCCGCCATGACGCCAGAACAGGCGCGGGAAA‑‑TGTGCGATACCGCGCGCAAACTGGGCAAGGTGCTGGCCTACGGCTTTCACCATCGTTTTGCGCTCGATACGCAACAGCTGCGTGAACAGGTGACCaa < 2:228076/136‑1 (MQ=255) ggcaaaaacCCGCCCGCCATGACGCCAGAACAGGCGCGGGAAA‑‑TGTGCGATACCGCGCGCAAACTGGGCAAGGTGCTGGCCTACGACTTTCACCATCGTTTTGCGCTCGATACGCAACAGCTGCGTGAACAGGTGACCaa < 2:304183/136‑1 (MQ=255) gCGAAAAACCGCCCGCCATGACGCCAGAACAGGCGCGGGAAA‑‑TGTGCGATACCGCGCGCAAACTGGGCAAGGTGCTGGCCTACGACTTTCACCATCGTTTTGCGCTCGATACGCAACAGCTGCGTGAACAGGTGACCAAc > 2:64109/1‑140 (MQ=255) gAAAAACCGCCCGCCATGACGCCAGAACAGGCGCGGGAAA‑‑TGTGCGATACCGCGCGCAAACTGGGCAAGGTGCTGGCCTACGACTTTCACCATCGTTTTGCGCTCGATACGCAACAGCTGCGTGAACAGGTGACCAACgg > 2:151441/1‑140 (MQ=255) gAAAAACCGCCCGCCATGACGCCAGAACAGGCGCGGGAAA‑‑TGTGCGATACCGCGCGCAAACTGGGCAAGGTGCTGGCCTACGACTTTCACCATCGTTTTGCGCTCGATACGCAACAGCTGCGTGAACAGGTGACCAACgg < 1:697218/140‑1 (MQ=255) aaaaCCGCCCGCCATGACGCCAGAACAGGCGCGGGAAA‑‑TGTGCGATACCGCGCGCAAACTGGGCAAGGTGCTGGCCTACGACTTTCACCATCGTTTTGCGCTCGATACGCAACAGCTGCGTGAACAGGTGACCAACGGCg > 1:325024/1‑140 (MQ=255) aaaaCCGCCCGCCATGACGCCAGAACAGGCGCGGGAAA‑‑TGTGCGATACCGCGCGCAAACTGGGCAAGGTGCTGGCCTACGACTTTCACCATCGTTTTGCGCTCGATACGCAACAGCTGCGTGAACAGGTGACCAACGGCg > 1:195349/1‑140 (MQ=255) aaCCGCCCGCCATGACGCCAGAACAGGCGCGGGAAA‑‑TGTGCGATACCGCGCGCAAACTGGGCAAGGTGCTGGCCTACGACTTTCACCATCGTTTTGCGCTCGATACGCAACAGCTGCGTGAACAGGTGACCAACGGCGtt < 2:195349/140‑1 (MQ=255) | ATGCATCCGTGTGGGACGATCCGCAGGCCATGCTGCTGGCGGTGAAACCTGATGTGGTTAGCGTCTGCTCACCTAACCGTTTTCATTACGAACATACCCTGATGGCACTGGAAGCGGGCTGCCATGTGATGTGCGAAAAACCGCCCGCCATGACGCCAGAACAGGCGCGGGAAA‑‑TGTGCGATACCGCGCGCAAACTGGGCAAGGTGCTGGCCTACGACTTTCACCATCGTTTTGCGCTCGATACGCAACAGCTGCGTGAACAGGTGACCAACGGCGTT > NC_000913/1377001‑1377278 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |