Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,825,0610GA30.6% 59.1 / 11.8 36intergenic (‑124/+79)ves/spyHutD family protein Ves/ATP‑independent periplasmic chaperone
Reads supporting (aligned to +/- strand):  ref base G (23/2);  new base A (0/11);  total (23/13)
Fisher's exact test for biased strand distribution p-value = 1.30e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

TATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCT  >  NC_000913/1824922‑1825195
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tatCAATGTCTTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTAGCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGGGATTTACGTTAGTGGGGATCAGGCTTTTTTTACAACTGTTTGAGAAGagaaaag                                                                                                                                        >  2:914429/1‑140 (MQ=255)
tatCAAGGTATTCCATACTCGCTCCAGGCCGGTTCTTTTCATTGTTTTCGTTCAGGTAACCGTGGATGTTTATCGGAAACTCGGGTGATTTACGTTGGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGagaaaag                                                                                                                                        <  2:1236469/140‑1 (MQ=255)
tatCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGagaaaag                                                                                                                                        <  2:949261/140‑1 (MQ=255)
  tCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGagaaaagaa                                                                                                                                      >  1:845227/1‑140 (MQ=255)
           tCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGa                                                                                                                             >  1:1019848/1‑140 (MQ=255)
           tCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGa                                                                                                                             >  1:376658/1‑140 (MQ=255)
           tCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGa                                                                                                                             >  1:1149632/1‑140 (MQ=255)
               tccccGCCCCCGGCCCGTTTTTTTATTTGTTATCGTTAAGGTAACGGTGGATGTTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCGGTTTTTTTTTAAACTTGTTTGAAAAGAAAAAAAAAAACCGCCGATCCt                                                                                                                         <  2:355827/136‑1 (MQ=255)
                    ggccccGGCCTGTTCTTTTGATTGTTTTGGTTGAGTTACCGGTGTATGGTTACCGGCACCTGGGGTAATTTATTTAAGGGGTATTCGGTTTTTTTTTACACTTGTTTGAAAAGAAAAAAGAAAACCGCCGATCCTGTCCa                                                                                                                    <  2:1177665/137‑1 (MQ=255)
                    gCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCa                                                                                                                    >  1:1091613/1‑140 (MQ=255)
                    gCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCa                                                                                                                    >  1:522540/1‑140 (MQ=255)
                    gCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCa                                                                                                                    >  1:553776/1‑140 (MQ=255)
                    gCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCa                                                                                                                    >  1:776007/1‑140 (MQ=255)
                     cTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCAc                                                                                                                   >  1:385701/1‑140 (MQ=255)
                     cTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCAc                                                                                                                   >  1:850063/1‑140 (MQ=255)
                       cccGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATAAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCg                                                                                                                 >  2:897402/1‑140 (MQ=255)
                            cccGTTTTTTTCATTTTTATCGTTCAGGTAACGGTGGATGTTTATCGGCAACTGGGGTGATTTACGTTAGTGGTGACCGGTTTTTTTTTACACCTGTTTGAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTa                                                                                                            <  2:823592/140‑1 (MQ=255)
                                 ttttttCTTTTTTATCTTTAAGGTACCGGTGGATGTTTATCGGCAACTGGGGTGTTTTACGTTAGTGGTGATCAGGTTTTTTTTACAACTTTTTGAAAAAAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCa                                                                                                       <  2:701317/138‑1 (MQ=255)
                                 ttttttCTTTTTTATCGTGCAGGTAACGGTGGATGTTTATCGGCAACGGGGGTGTTTTACGTTAGTGGTGATGAGGTTTTTTTTACAACTGTTTGAGAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCa                                                                                                       <  2:747039/138‑1 (MQ=255)
                                    tttCATTGTTATCGTTCAGGTAACCGTGGATGTTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGTTTTTTTTACAACTTTTTGAGAAGAAAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAgg                                                                                                    <  1:743277/140‑1 (MQ=255)
                                      tCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTa                                                                                                  >  2:1139678/1‑140 (MQ=255)
                                                    cAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACcgg                                                                                    >  1:1043211/1‑140 (MQ=255)
                                                      ggTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACcggcg                                                                                  >  1:1067409/1‑140 (MQ=255)
                                                      ggTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACcggcg                                                                                  >  1:965608/1‑140 (MQ=255)
                                                            cGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTa                                                                            >  1:1140039/1‑140 (MQ=255)
                                                                      tATCGGCAACTGGGGTGATTTACGTTAGTGGTGATCAGTTTTTTTTTACAACTGTTTGAGAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTgg                                                                  <  2:385701/140‑1 (MQ=255)
                                                                       aTCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGc                                                                 >  1:753738/1‑140 (MQ=255)
                                                                            cacctggggTGTTTTACGTTAGTGGTGATCGGTTTTTTTTTACATCTGTTTGAGAAAAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGaaa                                                            <  1:253002/134‑1 (MQ=255)
                                                                               cTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGat                                                         >  1:254665/1‑140 (MQ=255)
                                                                                 ggggTGTTTTACGTTGGTGGTGATCGGTTTTTTTTTAAAACTGTTTGAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGatta                                                       <  2:1140039/139‑1 (MQ=255)
                                                                                      taTTTACTTTGGTGGTGATAAGGTTTTTTTTACAATTGTTTGAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTcag                                                  <  1:897402/139‑1 (MQ=255)
                                                                                                  tggtgATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCa                                      >  1:379460/1‑140 (MQ=255)
                                                                                                      gATCAGGCTTTTTTTACAACTATTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCAtttt                                  >  1:391924/1‑140 (MQ=255)
                                                                                                       aTCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCAttttt                                 >  1:1272262/1‑139 (MQ=255)
                                                                                                              tttttttACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCttttt                          >  2:393255/1‑139 (MQ=255)
                                                                                                                 ttttACAATTTTTTGAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCg                       <  2:254665/140‑1 (MQ=255)
                                                                                                                        aCTTTTTGAGAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACg                <  2:434905/140‑1 (MQ=255)
                                                                                                                          tttttGAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGtt              <  2:1043211/138‑1 (MQ=255)
                                                                                                                          tGTTAGAGAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGtt              <  2:776007/140‑1 (MQ=255)
                                                                                                                              tGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGt          >  1:1139791/1‑140 (MQ=255)
                                                                                                                                  aaaaaaaaaaaaaaCCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGa      <  1:1139678/135‑1 (MQ=255)
                                                                                                                                    gagaaAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACg    >  1:597555/1‑140 (MQ=255)
                                                                                                                                     agaaaagaaaACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGc   >  1:70726/1‑140 (MQ=255)
                                                                                                                                      gaaaagaaaaCCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCt  >  1:834245/1‑140 (MQ=255)
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TATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCT  >  NC_000913/1824922‑1825195

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑