Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,604,3440CT45.2% 43.7 / 45.2 62S62F (TCT→TTT) bglJDNA‑binding transcriptional regulator BglJ
Reads supporting (aligned to +/- strand):  ref base C (6/28);  new base T (28/0);  total (34/28)
Fisher's exact test for biased strand distribution p-value = 4.46e-12
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

ATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGC‑GATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCAT  >  NC_000913/4604207‑4604481
                                                                                                                                         |                                                                                                                                          
aTGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATtttttttt                                                                                                                                          >  2:511489/1‑140 (MQ=255)
  gCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATtttttttttt                                                                                                                                        >  1:423902/1‑140 (MQ=255)
     tCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTttttctttttct                                                                                                                                     <  1:855972/140‑1 (MQ=255)
       aTGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGc                                                                                                                                   >  1:186191/1‑140 (MQ=255)
        tGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGcc                                                                                                                                  <  1:358244/140‑1 (MQ=255)
                aTCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAg                                                                                                                          <  1:247500/140‑1 (MQ=255)
                  cGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTg                                                                                                                        <  2:94102/140‑1 (MQ=255)
                    gTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGTCATGAGAAGTggg                                                                                                                      >  2:1009839/1‑138 (MQ=255)
                    gTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCATGAGAAGGGAg                                                                                                                      >  1:799715/1‑140 (MQ=255)
                    gTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTCTTCCCTGAGAAGTGAg                                                                                                                      >  2:1110983/1‑140 (MQ=255)
                    gTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAg                                                                                                                      >  1:349358/1‑140 (MQ=255)
                      aTTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCCTGAGAAGGGAgcg                                                                                                                    >  2:839501/1‑140 (MQ=255)
                      aTTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCATGAGAAGGGAggg                                                                                                                    >  2:500693/1‑138 (MQ=255)
                         gagaGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCATGAGAAGTGAGCGCag                                                                                                                 >  2:506158/1‑140 (MQ=255)
                         gagaGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCag                                                                                                                 <  1:543294/140‑1 (MQ=255)
                          agagTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCaga                                                                                                                <  1:1098249/140‑1 (MQ=255)
                           gagTTTATTCAGAAAGTTTGCGGGTAACCCGTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTGATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCagag                                                                                                               <  2:77882/140‑1 (MQ=255)
                              tttATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCGTACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCATGAGAAGTGAGCGCAGAGAgg                                                                                                            >  2:661581/1‑140 (MQ=255)
                              tttATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCATGAGAAGGGAGCGCAGAGAgg                                                                                                            >  2:431363/1‑140 (MQ=255)
                              tttATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCATGAGAAAGGAGCGCAGAgggg                                                                                                            >  2:775787/1‑140 (MQ=255)
                               ttATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAggg                                                                                                           <  2:361067/140‑1 (MQ=255)
                                tATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGGCATTTTTTTTTTTTTTGCCCTTTGGAGGGAGCGCAGAGggggg                                                                                                          >  2:202879/1‑139 (MQ=255)
                                 aTTCAGAAAGTTTGCAGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCATGAGAAGTGGGCGCAGAGAggggt                                                                                                         >  2:250158/1‑138 (MQ=255)
                                      gAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCATGAGAAGTGAGCGCAGAGAGGGATTATCt                                                                                                    >  1:491297/1‑140 (MQ=255)
                                      gAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCagag                                                                                                               >  1:753504/1‑129 (MQ=255)
                                      gAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCagag                                                                                                               <  2:753504/129‑1 (MQ=255)
                                                                    aTACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTTTTCCATGAGAAGTGAGCGCAGAGAGGGGTTATCTTGCCTGACTGAACTGGC‑GATTAAGttttcc                                                                     >  2:370988/1‑137 (MQ=255)
                                                                         tataCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGC‑GATTAAGTTTCCGCGTAc                                                                <  1:1105409/140‑1 (MQ=255)
                                                                          ataCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCATGAGAAGGGAGCGCAGAGAGGGGTTATCTTGCCTGACTGAACTGGG‑GGTTTAGTTTCCGCGTAcc                                                               >  2:357172/1‑140 (MQ=255)
                                                                          ataCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCATGAGAAGTGAGCGCAGAGAGGGGGTATTTTTCCTGACTGAACTGGC‑GATTAAATTTCCGCGTAcc                                                               >  2:140293/1‑140 (MQ=255)
                                                                           taCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCATTAGAAGTGAGGGCAGAGAGGGGTTATTTTTCCTGACTGAACTGGC‑GATTAAGTTTTCGCGTAccc                                                              >  2:1164458/1‑140 (MQ=255)
                                                                           taCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCATGAGAAGTGAGCGCAGAGAGGGGTTATCTTGCCTGACTGAACTGGT‑GATTAAGTTTTCGCGTAccc                                                              >  2:705262/1‑140 (MQ=255)
                                                                            aCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGC‑GATTAAGTTTCCGCGTACCCg                                                             <  1:1009839/140‑1 (MQ=255)
                                                                             ccAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGC‑GATTAAGTTTCCGCGTACCCgg                                                            >  2:1071429/1‑140 (MQ=255)
                                                                                gTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCCATTTTTTTTTTTTTGGCATCAGGAGTGTATGCAGAGCGGGGTTTTCCTACCCGCCAGAAATGGC‑GGTTAATTTTTCTCGTACCCCGcct                                                         >  2:458995/1‑138 (MQ=255)
                                                                                gTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCATGAGAAGTGAGCGCAGAGAGGGGTTATCTTGCCTGACTGAACTGGC‑GATTTAGTTTTCGCGTACCCcgcgg                                                         >  2:911207/1‑139 (MQ=255)
                                                                                gTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCATGAGAAGGGAGGGCAGAGAGGGGTTTTCTTGCCTGACTGAACTGGC‑GGTTAAGTTTTCGCGTACCCGGCgg                                                         >  2:741574/1‑139 (MQ=255)
                                                                                gTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTTTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGC‑GATTAAGTTTCCGCGTACCCGGCGt                                                         >  2:684031/1‑140 (MQ=255)
                                                                                 tCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCATGAGAAGGGAGGGCAGAGAGGGAGTATTTTCCCTGACTGAACTGGG‑GCCTTAGGTTTCGCGTTCCCgggggt                                                        >  2:501684/1‑136 (MQ=255)
                                                                                  cAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCATGAGAAGTGAGCGCAGAGAGGGGTTATCTTTCCTGACTGAACTGGC‑GATTAAATTTTCGCGTACCCCGCGttt                                                       >  2:1105409/1‑140 (MQ=255)
                                                                                  cAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTCTGCCATGAGAAGGGAGGGCAGAGAGGGATTATCTTGCCTGACTGAACTGGC‑GATTAAGTTTCCGCGTACCCGGCGttt                                                       >  1:828402/1‑140 (MQ=255)
                                                                                   aGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCATGAGAAGGGAGCGCAGAGAGGGGTTATCTTTCCTGACTGAACTGGC‑GATTAAGTTTTCGCGGACCCCGCGtttt                                                      >  2:438534/1‑139 (MQ=255)
                                                                                     gAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCCTTTTTTTTTTTTTTTCCCTGAAGAGGGGGCGCCGGGAGGGGGTATCTTGGCTGACTGAACAGGCGGATTAATTTTCCCGGACCCCGCCGtttgt                                                     >  2:544423/1‑138 (MQ=255)
                                                                                       gTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGC‑GATTAAGTTTCCGCGTACCCGGCGTTTAGTTa                                                  <  2:1111027/140‑1 (MQ=255)
                                                                                          aTTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGC‑GATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTg                                               <  1:501684/140‑1 (MQ=255)
                                                                                          aTTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGC‑GATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTg                                               <  2:86287/140‑1 (MQ=255)
                                                                                          aTTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGC‑GATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTg                                               <  1:82596/140‑1 (MQ=255)
                                                                                          aTTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGC‑GATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTg                                               <  1:839501/140‑1 (MQ=255)
                                                                                          aTTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGC‑GATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTg                                               <  2:349358/140‑1 (MQ=255)
                                                                                          aTTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGC‑GATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTg                                               <  1:1246807/140‑1 (MQ=255)
                                                                                          aTTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGGCATTTTTTTTTTTTTTGTCATCAGGAGGGAGAGGGGAGAGGGGTTTTTTTTCCTTACTGTAAAGGC‑GATTTAGTTTTCGCGTACCCGGCGGGTTGTTAttt                                               >  2:82596/1‑139 (MQ=255)
                                                                                            ttCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGC‑GATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCg                                             <  1:890655/140‑1 (MQ=255)
                                                                                            ttCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGC‑GATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCg                                             <  1:998229/140‑1 (MQ=255)
                                                                                              cAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGC‑GATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGga                                           <  1:1164458/140‑1 (MQ=255)
                                                                                                 gATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGC‑GATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGgatga                                        <  1:202879/140‑1 (MQ=255)
                                                                                                  aTGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGC‑GATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGgatgat                                       <  1:495710/140‑1 (MQ=255)
                                                                                                  aTGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGC‑GATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGgatgat                                       <  1:500693/140‑1 (MQ=255)
                                                                                                    gCCATGTCGCGGATCTCGTTTGCGGCGGTCCTTTTTTCTTTTTTTGCCATGAGAAGTGAGCGCAGAGGGGGATTATCTTGCCTGACTGAACTGGC‑GATTAAGTTTCCGCGGACCCGGCGGTTAGTTATTGCGggtggtgg                                     >  2:1207821/1‑139 (MQ=255)
                                                                                                      cATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCCTGAGAAGGGAGCGCCGAGGGGGATTATCTTTCCTGACTGAACTGGG‑GATTTAGGTTTCGCGTACCCGGCGGTTAGTTTTTTGGGGTGATGata                                   >  2:441760/1‑140 (MQ=255)
                                                                                                       aTGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGC‑GATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGatat                                  <  1:982363/140‑1 (MQ=255)
                                                                                                             cgGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGC‑GATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGc                            <  1:357172/140‑1 (MQ=255)
                                                                                                                 tctcGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGC‑GATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCgg                        <  2:186191/140‑1 (MQ=255)
                                                                                                                   tcGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGC‑GATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCt                      <  2:388337/140‑1 (MQ=255)
                                                                                                                          cggcggTCATTTTTTTTTTTTTTTCCATGAGAAGTGAGCGCAGAGAGGGGTTATCTTGCCTGACTTAACTGGC‑GATTAAGTTTTCGCGGACCCGGCGGTTTGTTTTTGCGGATGATGATATTTAAGCTCGGCtggttggt               >  2:836239/1‑140 (MQ=255)
                                                                                                                            gcggTCATTTTTTTTTTTTTTTCCCTGAGAAGTGAGCGCAGAGAGGGGTTATCTTGCCTGACTGAACTGGG‑GGTTAAATTTTCCCGTACCCGGCGGTTTGTTTTTTCGGGTGATGATATTTAAGCTCGGCTGAtttggtt             >  2:1144458/1‑135 (MQ=255)
                                                                                                                              ggTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGC‑GATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCAt           <  1:458995/140‑1 (MQ=255)
                                                                                                                                       ttctttttctGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGC‑GATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCAt  <  1:544423/140‑1 (MQ=255)
                                                                                                                                       ttctttttctGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGC‑GATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCAt  <  2:828402/140‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                          
ATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGC‑GATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCAT  >  NC_000913/4604207‑4604481

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑