Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	131,531	C→A	intergenic (‑271/‑84)	yacH ← / → acnB	DUF3300 domain‑containing protein YacH/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	131,531	0	C	A	100.0%	278.4 / NA	78	intergenic (‑271/‑84)	yacH/acnB	DUF3300 domain‑containing protein YacH/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (42/36);  total (42/36)

CGAATTAACGAAGTTTTTACGGAGGGAAACAATCTCTAGACCATCCTTAACGATTCAGCCACTTTTTTATGTTGCTTTTTTGTAAACAGATTAACACCTCGTCAAAATCCTGCTATTCTGCCCGTTGCGGTACTGGGCATTTACCCTACAAACTGCTGTCTCACAGGAGCGTGAAGAGAATCGCCTGCCGCACTATGACAATGAGAGCGAGGAGAACCGTCGTGCTAGAAGAATACCGTAAGCACGTAGCTGAGCGTGCCGCTGAGGGGATT  >  NC_000913/131394‑131665                                                                                                                                          |                                                                                                                                       cGAATTAACGAAGTTTTTACGGAGGGAAACAATCTCTAGACCATCCTTAACGATTCAGCCACTTTTTTATGTTGCTTTTTTGTAAACAGATTAACACCTCGTCAAAATCCTGCTATTCTGCCCGTTGCGGTACTGGGAAt                                                                                                                                      >  1:1234995/1‑140 (MQ=255)      tAACGAAGTTTTTACGGAGGGAAACAATCTCTAGACCATCCTTAACGATTCAGCCACTTTTTTATGTTGCTTTTTTGTAAACAGATTAACACCTCGTCAAAATCCTGCTATTCTGCCCGTTGCGGTACTGGGAATTTAcc                                                                                                                                 <  1:1113975/140‑1 (MQ=255)           aaGTTTTTACGGAGGGAAACAATCTCTAGACCATCCTTAACGATTCAGCCACTTTTTTATGTTGCTTTTTTGTAAACAGATTAACACCTCGTCAAAATCCTGCTATTCTGCCCGTTGCGGTACTGGGAATTTACCCTACa                                                                                                                            <  1:4869/140‑1 (MQ=255)            aGTTTTTACGGAGGGAAACAATCTCTAGACCATCCTTAACGATTCAGCCACTTTTTTATGTTGCTTTTTTGTAAACAGATTAACACCTCGTCAAAATCCTGCTATTCTGCCCGTTGCGGTACTGGGAATTTACCCTACaa                                                                                                                           <  2:958111/140‑1 (MQ=255)                 ttACGGAGGGAAACAATCTCTAGACCATCCTTAACGATTCAGCCACTTTTTTATGTTGCTTTTTTGTAAACAGATTAACACCTCGTCAAAATCCTGCTATTCTGCCCGTTGCGGTACTGGGAATTTACCCTACAAActgc                                                                                                                      <  1:356177/140‑1 (MQ=255)                 ttACGGAGGGAAACAATCTCTAGACCATCCTTAACGATTCAGCCACTTTTTTATGTTGCTTTTTTGTAAACAGATTAACACCTCGTCAAAATCCTGCTATTCTGCCCGTTGCGGTACTGGGAATTTACCCTAAAAActgc                                                                                                                      <  2:663101/140‑1 (MQ=255)                        gggAAACAATCTCTAGACCATCCTTAACGATTCAGCCACTTTTTTATGTTGCTTTTTTGTAAACAGATTAACACCTCGTCAAAATCCTGCTATTCTGCCCGTTGCGGTACTGGGAATTTACCCTACAAACTGCTGTCTca                                                                                                               >  2:1186682/1‑140 (MQ=255)                           aaaCAATCTCTAGACCATCCTTAACGATTCAGCCACTTTTTTATGTTGCTTTTTTGTAAACAGATTAACACCTCGTCAAAATCCTGCTATTCTGCCCGTTGCGGTACTGGGAATTTACCCTACAAACTGCTGTCTCACAg                                                                                                            <  1:1184807/140‑1 (MQ=255)                                   tctAGACCATCCTTAACGATTCAGCCACTTTTTTATGTTGCTTTTTTGTAAACAGATTAACACCTCGTCAAAATCCTGCTATTCTGCCCGTTGCGGTACTGGGAATTTACCCTACAAACTGCTGTCTCACAGGAGCGTGa                                                                                                    >  1:910948/1‑140 (MQ=255)                                           atcattAACGATTCAGCCACTTTTTTATGTTGCTTTTTTGTAAACAGATTAACACCTCGTCAAAATCCTGCTATTCTGCCCGTTGCGGTACTGGGAATTTACCCTACAAACTGCTGTCTCACAGGAGCGTGAAGAGAATc                                                                                            >  1:461574/5‑140 (MQ=255)                                           aTCCTTAACGATTCAGCCACTTTTTTATGTTGCTTTTTTGTAAACAGATTAACACCTCGTCAAAATCCTGCTATTCTGCCCGTTGCGGTACTGGGAATTTACCCTACAAACTGCTGTCTCACAGGAGCGTGAAGAGAATc                                                                                            >  1:689319/1‑140 (MQ=255)                                            tCCTTAACGATTCAGCCACTTTTTTATGTTGCTTTTTTGTAAACAGATTAACACCTCGTCAAAATCCTGCTATTCTGCCCGTTGCGGTACTGGGAATTTACCCTACAAACTGCTGTCTCACAGGAGCGTGa                                                                                                    <  1:78190/131‑1 (MQ=255)                                            tCCTTAACGATTCAGCCACTTTTTTATGTTGCTTTTTTGTAAACAGATTAACACCTCGTCAAAATCCTGCTATTCTGCCCGTTGCGGTACTGGGAATTTACCCTACAAACTGCTGTCTCACAGGAGCGTGa                                                                                                    >  2:78190/1‑131 (MQ=255)                                            tCCTTAACGATTCAGCCACTTTTTTATGTTGCTTTTTTGTAAACAGATTAACACCTCGTCAAAATCCTGCTATTCTGCCCGTTGCGGTACTGGGAATTTACCCTACAAACTGCTGTCTCACAGGAGCGTGAAGAGAATCg                                                                                           >  1:751333/1‑140 (MQ=255)                                             ccTTAACGATTCAGCCACTTTTTTATGTTGCTTTTTTGTAAACAGATTAACACCTCGTCAAAATCCTGCTATTCTGCCCGTTGCGGTACTGGGAATTTACCCTACAAACTGCTGTCTCACAGGAGCGTGAAGAGAATCGc                                                                                          >  1:883632/1‑140 (MQ=255)                                             ccTTAACGATTCAGCCACTTTTTTATGTTGCTTTTTTGTAAACAGATTAACACCTCGTCAAAATCCTGCTATTCTGCCCGTTGCGGTACTGGGAATTTACCCTACAAACTGCTGTCTCACAGGAGCGTGAAGAGAATCGc                                                                                          >  2:486658/1‑140 (MQ=255)                                             ccTTAACGATTCAGCCACTTTTTTATGTTGCTTTTTTGTAAACAGATTAACACCTCGTCAAAATCCTGCTATTCTGCCCGTTGCGGTACTGGGAATTTACCCTACAAACTGCTGTCTCACAGGAGCGTGAAGAGAATCGc                                                                                          >  1:1266587/1‑140 (MQ=255)                                             ccTTAACGATTCAGCCACTTTTTTATGTTGCTTTTTTGTAAACAGATTAACACCTCGTCAAAATCCTGCTATTCTGCCCGTTGCGGTACTGGGAATTTACCCTACAAACTGCTGTCTCACAGGAGCGTGAAGAGAATCGc                                                                                          >  1:162862/1‑140 (MQ=255)                                             ccTTAACGATTCAGCCACTTTTTTATGTTGCTTTTTTGTAAACAGATTAACACCTCGTCAAAATCCTGCTATTCTGCCCGTTGCGGTACTGGGAATTTACCCTACAAACTGCTGTCTAACAGGAGCGTGAAGAGAATCGc                                                                                          <  2:194107/140‑1 (MQ=255)                                                 aaCGATTCAGCCACTTTTTTATGTTGCTTTTTTGTAAACAGATTAACACCTCGTCAAAATCCTGCTATTCTGCCCGTTGCGGTACTGGGAATTTACCCTACAAACTGCTGTCTCACAGGAGCGTGAAGAGAATCGCCTGc                                                                                      <  2:461574/140‑1 (MQ=255)                                                 aaCGATTCAGCCACTTTTTTATGTTGCTTTTTTGTAAACAGATTAACACCTCGTCAAAATCCTGCTATTCTGCCCGTTGCGGTACTGGGAATTTACCCTACAAACTGCTGTCTCACAGGAGCGTGAAGAGAATCGCCTGc                                                                                      <  2:1266587/140‑1 (MQ=255)                                                   cGATTCAGCCACTTTTTTATGTTGCTTTTTTGTAAACAGATTAACACCTCGTCAAAATCCTGCTATTCTGCCCGTTGCGGTACTGGGAATTTACCCTACAAACTGCTGTCTCACAGGAGCGTGAAGAGAATCGCCTGCCg                                                                                    <  2:751333/140‑1 (MQ=255)                                                      ttCAGCCACTTTTTTATGTTGCTTTTTTGTAAACAGATTAACACCTCGTCAAAATCCTGCTATTCTGCCCGTTGCGGTACTGGGAATTTACCCTACAAACTGCTGTCTCACAGGAGCGTGAAGAGAATCGCCTGCCGCAc                                                                                 >  1:892185/1‑140 (MQ=255)                                                      ttCAGCCACTTTTTTATGTTGCTTTTTTGGAAACAGATTAACACCTCGTCAAAATCCTGCTATTCTGCCCGTTGCGGTACTGGGAATTTACCCTACAAACTGCTGTCTCACAGGAGCGTGAAGAGAATCGCCTGCCGCAc                                                                                 >  2:588388/1‑140 (MQ=255)                                                       tCAGCCACTTTTTTATGTTGCTTTTTTGTAAACAGATTAACACCTCGTCAAAATCCTGCTATTCTGCCCGTTGCGGTACTGGGAATTTACCCTACAAACTGCTGTCTCACAGGAGCGTGAAGAGAATCGCCTGCCGCACt                                                                                <  2:433563/140‑1 (MQ=255)                                                       tCAGCCACTTTTTTATGTTGCTTTTTTGTAAACAGATTAACACCTCGTCAAAATCCTGCTATTCTGCCCGTTGCGGTACTGGGAATTTACCCTACAAACTGCTGTCTCACAGGAGCGTGAAGAGAATCGCCTGCCGCACt                                                                                <  2:447155/140‑1 (MQ=255)                                                       tCAGCCACTTTTTTATGTTGCTTTTTTGTAAACAGATTAACACCTCGTCAAAATCCTGCTATTCTGCCCGTTGCGGTACTGGGAATTTACCCTACAAACTGCTGTCTCACAGGAGCGTGAAGAGAATCGCCTGCCGCACt                                                                                <  2:1107641/140‑1 (MQ=255)                                                       tCAGCCACTTTTTTATGTTGCTTTTTTGTAAACAGATTAACACCTCGTCAAAATCCTGCTATTCTGCCCGTTGCGGTACTGGGAATTTACCCTACAAACTGCTGTCTCACAGGAGCGTGAAGAGAATCGCCTGCCGCACt                                                                                <  2:883632/140‑1 (MQ=255)                                                         aGCCACTTTTTTATGTTGCTTTTTTGTAAACAGATTAACACCTCGTCAAAATCCTGCTATTCTGCCCGTTGCGGTACTGGGAATTTACCCTACAAACTGCTGTCTCACAGGAGCGTGAAGAGAATCGCCTGCCGCACTAt                                                                              >  2:536115/1‑140 (MQ=255)                                                           ccACTTTTTTATGTTGCTTTTTTGTAAACAGATTAACACCTCGTCAAAATCCTGCTATTCTGCCCGTTGCGGTACTGGGAATTTACCCTACAAACTGCTGTCTCACAGGAGCGTGAAGAGAATCGCCTGCCGCACTATGa                                                                            >  1:590702/1‑140 (MQ=255)                                                                   ttATGTTGCTTTTTTGTAAACAGATTAACACCTCGTCAAAATCCTGCTATTCTGCCCGTTGCGGTACTGGGAATTTACCCTACAAACTGCTGTCTCACAGGAGCGTGAAGAGAATCGCCTGCCGCACTATGACAATgaga                                                                    <  2:689319/140‑1 (MQ=255)                                                                   ttATGTTGCTTTTTTGTAAACAGATTAACACCTCGTCAAAATCCTGCTATTCTGCCCGTTGCGGTACTGGGAATTTACCCTACAAACTGCTGTCTCACAGGAGCGTGAAGAGAATCGCCTGCCGCACTATGACAATgaga                                                                    <  1:327759/140‑1 (MQ=255)                                                                   ttATGTTGCTTTTTTGTAAACAGATTAACACCTCGTCAAAATCCTGCTATTCTGCCCGTTGCGGTACTGGGAATTTACCCTACAAACTGCTGTCTCACAGGAGCGTGAAGAGAATCGCCTGCCGCACTATGACAATgaga                                                                    <  2:573263/140‑1 (MQ=255)                                                                    tATGTTGCTTTTTTGTAAACAGATTAACACCTCGTCAAAATCCTGCTATTCTGCCCGTTGCGGTACTGGGAATTTACCCTACAAACTGCTGTCTCACAGGAGCGTGAAGAGAATCGCCTGCCGCACTATGACAATGagag                                                                   <  2:748346/140‑1 (MQ=255)                                                                    tATGTTGCTTTTTTGTAAACAGATTAACACCTCGTCAAAATCCTGCTATTCTGCCCGTTGCGGTACTGGGAATTTACCCTACAAACTGCTGTCTCACAGGAGCGTGAAGAGAATCGCCTGCCGCACTATGACAATGagag                                                                   >  2:529675/1‑140 (MQ=255)                                                                      tGTTGCTTTTTTGTAAACAGATTAACACCTCGTCAAAATCCTGCTATTCTGCCCGTTGCGGTACTGGGAATTTACCCTACAAACTGCTGTCTCACAGGAGCGTGAAGAGAATCGCCTGCCGCACTATGACAATGAGAGCg                                                                 <  1:907002/140‑1 (MQ=255)                                                                      tGTTGCTTTTTTGTAAACAGATTAACACCTCGTCAAAATCCTGCTATTCTGCCCGTTGCGGTACTGGGAATTTACCCTACAAACTGCTGTCTCACAGGAGCGTGAAGAGAATCGCCTGCCGCACTATGACAATGAGAGCg                                                                 <  1:486658/140‑1 (MQ=255)                                                                         tGCTTTTTTGTAAACAGATTAACACCTCGTCAAAATCCTGCTATTCTGCCCGTTGCGGTACTGGGAATTTACCCTACAAACTGCTGTCTCACAGGAGCGTGAAGAGAATCGCCTGCCGCACTATGACAATGAGAGCgagg                                                              >  1:473219/1‑140 (MQ=255)                                                                         tGCTTTTTTGTAAACAGATTAACACCTCGTCAAAATCCTGCTATTCTGCCCGTTGCGGTACTGGGAATTTACCCTACAAACTGCTGTCTCACAGGAGCGTGAAGAGAATCGCCTGCCGCACTATGACAATGAGAGCgagg                                                              >  1:1028291/1‑140 (MQ=255)                                                                         tGCTTTTTTGTAAACAGATTAACACCTCGTCAAAATCCTGCTATTCTGCCCGTTGCGGTACTGGGAATTTACCCTACAAACTGCTGTCTCACAGGAGCGTGAAGAGAATCGCCTGCCGCACTATGACAATGAGAGCgagg                                                              >  2:138065/1‑140 (MQ=255)                                                                           cTTTTTTGTAAACAGATTAACACCTCGTCAAAATCCTGCTATTCTGCCCGTTGCGGTACTGGGAATTTACCCTACAAACTGCTGTCTCACAGGAGCGTGAAGAGAATCGCCTGCCGCACTATGACAATGAGAGCgaggag                                                            >  2:1254003/1‑140 (MQ=255)                                                                           cTTTTTTGTAAACAGATTAACACCTCGTCAAAATCCTGCTATTCTGCCCGTTGCGGTACTGGGAATTTACCCTACAAACTGCTGTCTCACAGGAGCGTGAAGAGAATCGCCTGCCGCACTATGACAATGAGAGCgaggag                                                            >  1:427184/1‑140 (MQ=255)                                                                                 tGTAAACAGATTAACACCTCGTCAAAATCCTGCTATTCTGCCCGTTGCGGTACTGGGAATTTACCCTACAAACTGCTGTCTCACAGGAGCGTGAAGAGAATCGCCTGCCGCACTATGACAATGAGAGCGAGGAGAACcgt                                                      <  2:578269/140‑1 (MQ=255)                                                                                 tGTAAACAGATTAACACCTCGTCAAAATCCTGCTATTCTGCCCGTTGCGGTACTGGGAATTTACCCTACAAACTGCTGTCTCACAGGAGCGTGAAGAGAATCGCCTGCCGCACTATGACAATGAGAGCGAGGAGAACcgt                                                      <  2:992444/140‑1 (MQ=255)                                                                                    aaaCAGATTAACACCTCGTCAAAATCCTGCTATTCTGCCCGTTGCGGTACTGGGAATTTACCCTACAAACTGCTGTCTCACAGGAGCGTGAAGAGAATCGCCTGCCGCACTATGACAATGAGAGCGAGGAGAACcgtcgt                                                   <  2:427184/140‑1 (MQ=255)                                                                                     aaCAGATTAACACCTCGTCAAAATCCTGCTATTCTGCCCGTTGCGGTACTGGGAATTTACCCTACAAACTGCTGTCTCACAGGAGCGTGAAGAGAATCGCCTGCCGCACTATGACAATGAGAGCGAGGAGAACCGTCGTg                                                  >  1:554282/1‑140 (MQ=255)                                                                                          aTTAACTCCTCGTCAAAATCCTGCTATTCTGCCCGTTGCGGTACTGGGAATTTACCCTACAAACTGCTGTCTCACAGGAGCGTGAAGAGAATCGCCTGCCGCACTATGACAATGAGAGCGAGGAGAACCGTCGTGCTaga                                             <  1:728556/140‑1 (MQ=255)                                                                                          aTTAACACCTCGTCAAAATCCTGCTATTCTGCCCGTTGCGGTACTGGGAATTTACCCTACAAACTGCTGTCTCACAGGAGCGTGAAGAGAATCGCCTGCCGCACTATGACAATGAGAGCGAGgaga                                                           <  1:173892/126‑1 (MQ=255)                                                                                          aTTAACACCTCGTCAAAATCCTGCTATTCTGCCCGTTGCGGTACTGGGAATTTACCCTACAAACTGCTGTCTCACAGGAGCGTGAAGAGAATCGCCTGCCGCACTATGACAATGAGAGCGAGgaga                                                           >  2:173892/1‑126 (MQ=255)                                                                                          aTTAACACCTCGTCAAAATCCTGCTATTCTGCCCGTTGCGGTACTGGGAATTTACCCTACAAACTGCTGTCTCACAGGAGCGTGAAGAGAATCGCCTGCCGCACTATGACAATGAGAGCGAGGAGAACCGTCGTGCTaga                                             >  2:145851/1‑140 (MQ=255)                                                                                             aaCACCTCGTCAAAATCCTGCTATTCTGCCCGTTGCGGTACTGGGAATTTACCCTACAAACTGCTGTCTCACAGGAGCGTGAAGAGAATCGCCTGCCGCACTATGACAATGAGAGCGAGGAGAACCGTCGTGCTagaaga                                          <  2:910948/140‑1 (MQ=255)                                                                                                  cTCGTCAAAATCCTGCTATTCTGCCCGTTGCGGTACTGGGAATTTACCCTACAAACTGCTGTCTCACAGGAGCGTGAAGAGAATCGCCTGCCGCACTATGACAATGAGAGCGAGGAGAACCGTCGTGCTAGAAGAATAcc                                     >  1:303283/1‑140 (MQ=255)                                                                                                  cTCGTCAAAATCCTGCTATTCTGCCCGTTGCGGTACTGGGAATTTACCCTACAAACTGCTGTCTCACAGGAGCGTGAAGAGAATCGCCTGCCGCACTATGACAATGAGAGCGAGGAGAACCGTCGTGCTAGAAGAATAcc                                     >  1:923044/1‑140 (MQ=255)                                                                                                    cGTCAAAATCCTGCTATTCTGCCCGTTGCGGTACTGGGAATTTACCCTACAAACTGCTGTCTCACAGGAGCGTGAAGAGAATCGCCTGCCGCACTATGACAATGAGAGCGAGGAGAACcgtcg                                                    <  2:882097/123‑1 (MQ=255)                                                                                                    cGTCAAAATCCTGCTATTCTGCCCGTTGCGGTACTGGGAATTTACCCTACAAACTGCTGTCTCACAGGAGCGTGAAGAGAATCGCCTGCCGCACTATGACAATGAGAGCGAGGAGAACcgtcg                                                    >  1:882097/1‑123 (MQ=255)                                                                                                    cGTCAAAATCCTGCTATTCTGCCCGTTGCGGTACTGGGAATTTACCCTACAAACTGCTGTCTCACAGGAGCGTGAAGAGAATCGCCTGCCGCACTATGACAATGAGAGCGAGGAGAACCGTTGTGCTAGAAGAATACCGt                                   >  2:882334/1‑140 (MQ=255)                                                                                                    cGTCAAAATCCTGCTATTCTGCCCGTTGCGGTACTGGGAATTTACCCTACAAACTGCTGTCTCACAGGAGCGTGAAGAGAATCGCCTGCCGCACTATGACAATGAGAGCGAGGAGAACCGTCGTGc                                                 >  1:498652/1‑126 (MQ=255)                                                                                                    cGTCAAAATCCTGCTATTCTGCCCGTTGCGGTACTGGGAATTTACCCTACAAACTGCTGTCTCACAGGAGCGTGAAGAGAATCGCCTGCCGCACTATGACAATGAGAGCGAGGAGAACCGTCGTGc                                                 <  2:498652/126‑1 (MQ=255)                                                                                                    cGTCAAAATCCTGCTATTCTGCCCGTTGCGGTACTGGGAATTTACCCTACAAACTGCTGTCTCACAGGAGCGTGAAGAGAATCGCCTGCCGCACTATGACAATGAGAGCGAGGAGAACCGTCGTGCTAGAAGAATACCGt                                   >  1:608122/1‑140 (MQ=255)                                                                                                    cGTCAAAATCCTGCTATTCTGCCCGTTGCGGTACTGGGAATTTACCCTACAAACTGCTGTCTCACAGGAGCGTGAAGAGAATCGCCTGCCGCACTATGACAATGAGAGCGAGGAGAACCGTCGTGCTAGAAGAATACCGt                                   >  2:897629/1‑140 (MQ=255)                                                                                                    cGTCAAAATCCTGCTATTCTGCCCGTTGCGGTACTGGGAATTTACCCTACAAACTGCTGTCTCACAGGAGCGTGAAGAGAATCGCCTGCCGCACTATGACAATGAGAGCGAGGAGAACCGTCGTGCTAGAAGAATACCGt                                   >  1:599213/1‑140 (MQ=255)                                                                                                    cGTCAAAATCCTGCTATTCTGCCCGTTGCGGTACTGGGAATTTACCCTACAAACTGCTGTCTCACAGGAGCGTGAAGAGAATCGCCTGCCGCACTATGACAATGAGAGCGAGGAGAACCGTCGTGCTAGAAGAATACCGt                                   >  2:587849/1‑140 (MQ=255)                                                                                                    cGTCAAAATCCTGCTATTCTGCCCGTTGCGGTACTGGGAATTTACCCTACAAACTGCTGTCTCACAGGAGCGTGAAGAGAATCGCCTGCCGCACTATGACAATGAGAGCGAGGAGAACCGTCGTGCTAGAAGAATACCGt                                   >  2:1246532/1‑140 (MQ=255)                                                                                                    cGTCAAAATCCTGCTATTCTGCCCGTTGCGGTACTGGGAATTTACCCTACAAACTGCTGTCTCACAGGAGCGTGAAGAGAATCGCCTGCCGCACTATGACAATGAGAGCGAGGAGAACCGTCGTGCTAGAAGAATACCGt                                   >  1:144513/1‑140 (MQ=255)                                                                                                      tCAAAATCCTGCTATTCTGCCCGTTGCGGTACTGGGAATTTACCCTACAAACTGCTGTCTCACAGGAGCGTGAAGAGAATCGCCTGCCGCACTATGACAATGAGAGCGAGGAGAACCGTCGTGCTAGAAGAATACCGTaa                                 <  1:145851/140‑1 (MQ=255)                                                                                                      tCAAAATCCTGCTATTCTACCCGTTGCGGTACTGGGAATTTACCCTACAAACTGCTGTCTCACAGGAGCGTGAAGAGAATCGCCTGCCGCACTATGACAATGAGAGCGAGGAGAACCGTCGTGCTAGAAGAATACCGTaa                                 <  1:1218811/140‑1 (MQ=255)                                                                                                                gCTATTCTGCCCGTTGCGGTACTGGGAATTTACCCTACAAACTGCTGTCTCACAGGAGCGTGAAGAGAATCGCCTGCCGCACTATGACAATGAGAGCGAGGAGAACCGTCGTGCTAGAAGAATACCGTAAGCACGTAGCt                       >  1:330642/1‑140 (MQ=255)                                                                                                                 cTATTCTGCCCGTTGCGGTACTGGGAATTTACCCTACAAACTGCTGTCTCACAGGAGCGTGAAGAGAATCGCCTGCCGCACTATGACAATGAGAGCGAGGAGAACCGTCGTGCTAGAAGAATACCGTAAGCACGTAGCTg                      >  2:277700/1‑140 (MQ=255)                                                                                                                     tCTGCCCGTTGCGGTACTGGGAATTTACCCTACAAACTGCTGTCTCACAGGAGCGTGAAGAGAATCGCCTGCCGCACTATGACAATGAGAGCGAGGAGAACCGTTGTGCTAGAAGAATACCGTAAGCACGTAGCTGAGCg                  <  1:882334/140‑1 (MQ=255)                                                                                                                      cTGCCCGTTGCGGTACTGGGAATTTACCCTACAAACTGCTGTCTCACAGGGGCGTGAAGAGAATCGCCTGCCGCACTATGACAATGAGAGCGAGGAGAACCGTCGTGCTAGAAGAATACCGTAAGCACGTAGCTGAGCGt                 >  1:48472/1‑140 (MQ=255)                                                                                                                       tGCCCGTTGCGGTACTGGGAATTTACCCTACAAACTGCTGTCTCACAGGAGCGTGAAGAGAATCGCCTGCCGCACTATGACAATGAGAGCGAGGAGAACCGTCGTGCTAGAAGAATACCGTAAGCACGTAGCTGAGCGTg                <  1:588388/140‑1 (MQ=255)                                                                                                                         cccGTTGCGGTACTGGGAATTTACCCTACAAACTGCTGTCTCACAGGAGCGTGAAGAGAATCGCCTGCCGCACTATGACAATGAGAGCGAGGAGAACCGTCGTGCTAGAAGAATACCGTAAGCACGTAGCTGAGCGTGcc              >  2:321780/1‑140 (MQ=255)                                                                                                                         cccGTTGCGGTACTGGGAATTTACCCTACAAACTGCTGTCTCACAGGAGCGTGAAGAGAATCGCCTGCCGCACTATGACAATGAGAGCGAGGAGAACCGTCGTGCTAGAAGAATACCGTAAGCACGTAGCTGAGCGTGcc              >  2:471210/1‑140 (MQ=255)                                                                                                                             ttGCGGTACTGGGAATTTACCCTACAAACTGCTGTCTCACAGGAGCGTGAAGAGAATCGCCTGCCGCACTATGACAATGAGAGCGAGGAGAACCGTCGTGCTAGAAGAATACCGTAAGCACGTAGCTGAGCGTGCCGCTg          <  1:471210/140‑1 (MQ=255)                                                                                                                              tGCGGTACTGGGAATTTACCCTACAAACTGCTGTCTCACAGGAGCGTGAAGAGAATCGCCTGCCGCACTATGACAATGAGAGCGAGGAGAACCGTCGTGCTAGAAGAATACCGTAAGCACGTAGCTGAGCGTGCCGCTGa         >  1:233816/1‑140 (MQ=255)                                                                                                                               gCGGTACTGGGAATTTACCCTACAAACTGCTGTCTCACAGGAGCGTGAAGAGAATCGCCTGCCGCACTATGACAATGAGAGCGAGGAGAACCGTCGTGCTAGAAGAATACCGTAAGCACGTAGCTGAGCGTGCCGCTGAg        <  1:529675/140‑1 (MQ=255)                                                                                                                                cGGTACTGGGAATTTACCCTACAAACTGCTGTCTCACAGGAGCGTGAAGAGAATCGCCTGCCGCACTATGACAATGAGAGCGAGGAGAACCGTCGTGCTAGAAGAATACCGTAAGCACGTAGCTGAGCGTGCCGCTGAgg       >  1:1163534/1‑140 (MQ=255)                                                                                                                                     cTGGGAATTTACCCTACAAACTGCTGTCTCACAGGAGCGTGAAGAGAATCGCCTGCCGCACTATGACAATGAGAGCGAGGAGAACCGTCGTGCTAGAAGAATACCGTAAGCACGTAGCTGAGCGTGCCGCTGAGGGGAtt  <  1:587849/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                       CGAATTAACGAAGTTTTTACGGAGGGAAACAATCTCTAGACCATCCTTAACGATTCAGCCACTTTTTTATGTTGCTTTTTTGTAAACAGATTAACACCTCGTCAAAATCCTGCTATTCTGCCCGTTGCGGTACTGGGCATTTACCCTACAAACTGCTGTCTCACAGGAGCGTGAAGAGAATCGCCTGCCGCACTATGACAATGAGAGCGAGGAGAACCGTCGTGCTAGAAGAATACCGTAAGCACGTAGCTGAGCGTGCCGCTGAGGGGATT  >  NC_000913/131394‑131665

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑