Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,233,498	C→A	V207V (GTC→GTA)	sanA →	DUF218 domain‑containing protein SanA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,233,498	0	C	A	100.0%	264.9 / NA	78	V207V (GTC→GTA)	sanA	DUF218 domain‑containing protein SanA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (34/44);  total (34/44)

TTCAGGCTCAGTGTTATGCCGTACCGTCACCGAAAGATATGCTGTCAGTACGTATTCGTGAATTTGCCGCCCGTTTCGGTGCGCTGGCTGACCTTTATATTTTTAAACGTGAACCGCGTTTTTTAGGGCCGCTGGTCCCTATTCCGGCTATGCACCAGGTACCGGAAGATGCGCAGGGGTATCCCGCCGTCACACCCGAACAGTTACTTGAATTACAAAAGAAACAAGGAAAGTAGTTATGGATGTACAGCAGTTTTTTGTCGTTGCCGTTTT  >  NC_000913/2233362‑2233634                                                                                                                                         |                                                                                                                                         ttCAGGCTCAGTGTTATGCCGTACCGTCACCGAAAGATATGCTGTCAGTACGTATTCGTGAATTTGCCGCCCGTTTCGGTGCGCTGGCTGACCTTTATATTTTTAAACGTGAACCGCGTTTTTTAGGGCCGCTGGTc                                                                                                                                          <  2:9553/137‑1 (MQ=255) ttCAGGCTCAGTGTTATGCCGTACCGTCACCGAAAGATATGCTGTCAGTACGTATTCGTGAATTTGCCGCCCGTTTCGGTGCGCTGGCTGACCTTTATATTTTTAAACGTGAACCGCGTTTTTTAGGGCCGCTGGTc                                                                                                                                          >  1:9553/1‑137 (MQ=255) ttCAGGCTCAGTGTTATGCCGTACCGTCACCGAAAGATATGCTGTCAGTACGTATTCGTGAATTTGCCGCCCGTTTCGGTGCGCTGGCTGACCTTTATATTTTTAAACGTGAACCGCGTTTTTTAGGGCCGCTGGTACCt                                                                                                                                       >  1:183707/1‑140 (MQ=255)     ggCTCAGTGTTATGCCGTACCGTCACCGAAAGATATGCTGTCAGTACGTATTCGTGAATTTGCCGCCCGTTTCGGTGCGCTGGCTGACCTTTATATTTTTAAACGTGAACCGCGTTTTTTAGGGCCGCTGGTACCTATTc                                                                                                                                   <  1:1239253/140‑1 (MQ=255)      gCTCAGTGTTATGCCGTACCGTCACCGAAAGATATGCTGTCAGTACGTATTCGTGAATTTGCCGCCCGTTTCGGTGCGCTGGCTGACCTTTATATTTTTAAACGTGAACCGCGTTTTTTAGGGCCGCTGGTACCTATTcc                                                                                                                                  <  1:1025602/140‑1 (MQ=255)         cAGTGTTATGCCGTACCGTCACCGAAAGATATGCTGTCAGTACGTATTCGTGAATTTGCCGCCCGTTTCGGTGCGCTGGCTGACCTTTATATTTTTAAACGTGAACCGCGTTTTTTAGGGCCGCTGGTACCTATTCCGGc                                                                                                                               >  2:477566/1‑140 (MQ=255)         cAGTGTTATGCCGTACCGTCACCGAAAGATATGCTGTCAGTACGTATTCGTGAATTTGCCGCCCGTTTCGGTGCGCTGGCTGACCTTTATATTTTTAAACGTGAACCGCGTTTTTTAGGGCCGCTGGTACCTATTCCGGc                                                                                                                               >  2:1120588/1‑140 (MQ=255)            tgtTATGCCGTACCGTCACCGAAAGATATGCTGTCAGTACGTATTCGTGAATTTGCCGCCCGTTTCGGTGCGCTGGCTGACCTTTATATTTTTAAACGTGAACCGCGTTTTTTAGGGCCGCTGGTACCTATTCCGGCTAt                                                                                                                            >  1:1221165/1‑140 (MQ=255)            tgtTATGCCGTACCGTCACCGAAAGATATGCTGTCAGTACGTATTCGTGAATTTGCCGCCCGTTTCGGTGCGCTGGCTGACCTTTATATTTTTAAACGTGAACCGCGTTTTTTAGGGCCGCTGGTACCTATTCCGGCTAt                                                                                                                            <  1:449141/140‑1 (MQ=255)            tgtTATGCCGTACCGTCACCGAAAGATATGCTGTCAGTACGTATTCGTGAATTTGCCGCCCGTTTCGGTGCGCTGGCTGACCTTTATATTTTTAAACGTGAACCGCGTTTTTTAGGGCCGCTGGTACCTATTCCGGCTAt                                                                                                                            <  2:783246/140‑1 (MQ=255)                aTGCCGTACCGTCACCGAAAGATATGCTGTCAGTACGTATTCGTGAATTTGCCGCCCGTTTCGGTGCGCTGGCTGACCTTTATATTTTTAAACGTGAACCGCGTTTTTTAGGGCCGCTGGTACCTATTCCGGCTATGCAc                                                                                                                        <  2:540200/140‑1 (MQ=255)                aTGCCGTACCGTCACCGAAAGATATGCTGTCAGTACGTATTCGTGAATTTGCCGCCCGTTTCGGTGCGCTGGCTGACCTTTATATTTTTAAACGTGAACCGCGTTTTTTAGGGCCGCTGGTACCTATTCCGGCTATGCAc                                                                                                                        <  1:51089/140‑1 (MQ=255)                aTGCCGTACCGTCACCGAAAGATATGCTGTCAGTACGTATTCGTGAATTTGCCGCCCGTTTCGGTGCGCTGGCTGACCTTTATATTTTTAAACGTGAACCGCGTTTTTTAGGGCCGCTGGTACCTATTCCGGCTATGCAc                                                                                                                        <  1:423084/140‑1 (MQ=255)                    cGTACCGTCACCGAAAGATATGCTGTCAGTACGTATTCGTGAATTTGCCGCCCGTTTCGGTGCGCTGGCTGACCTTTATATTTTTAAACGTGAACCGCGTTTTTTAGGGCCGCTGGTACCTATTCCGGCTATGCACCAgg                                                                                                                    <  1:1098943/140‑1 (MQ=255)                      tACCGTCACCGAAAGATATGCTGTCAGTACGTATTCGTGAATTTGCCGCCCGTTTCGGTGCGCTGGCTGACCTTTATATTTTTAAACGTGAACCGCGTTTTTTAGGGCCGCTGGTACCTATTCCGGCt                                                                                                                              <  2:319966/128‑1 (MQ=255)                      tACCGTCACCGAAAGATATGCTGTCAGTACGTATTCGTGAATTTGCCGCCCGTTTCGGTGCGCTGGCTGACCTTTATATTTTTAAACGTGAACCGCGTTTTTTAGGGCCGCTGGTACCTATTCCGGCt                                                                                                                              >  1:319966/1‑128 (MQ=255)                      tACCGTCACCGAAAGATATGCTGTCAGTACGTATTCGTGAATTTGCCGCCCGTTTCGGTGCGCTGGCTGACCTTTATATTTTTAAACGTGAACCGCGTTTTTTAGGGCCGCTGGTACCTATTCCGGCTATGCACCAGGTa                                                                                                                  >  2:276357/1‑140 (MQ=255)                      tACCGTCACCGAAAGATATGCTGTCAGTACGTATTCGTGAATTTGCCGCCCGTTTCGGTGCGCTGGCTGACCTTTATATTTTTAAACGTGAACCGCGTTTTTTAGGGCCGCTGGTACCTATTCCGGCTATGCACCAGGTa                                                                                                                  >  2:42885/1‑140 (MQ=255)                      tACCGTCACCGAAAGATATGCTGTCAGTACGTATTCGTGAATTTGCCGCCCGTTTCGGTGCGCTGGCTGACCTTTATATTTTTAAACGTGAACCGCGTTTTTTAGGGCCGCTGGTACCTATTCCGGCTATGCACCAGGTa                                                                                                                  >  2:224577/1‑140 (MQ=255)                      tACCGTCACCGAAAGATATGCTGTCAGTACGTATTCGTGAATTTGCCGCCCGTTTCGGTGCGCTGGCTGACCTTTATATTTTTAAACGTGAACCGCGTTTTTTAGGGCCGCTGGTACCTATTCCGGCTATGCACCAGGTa                                                                                                                  >  2:776929/1‑140 (MQ=255)                      tACCGTCACCGAAAGATATGCTGTCAGTACGTATTCGTGAATTTGCCGCCCGTTTCGGTGCGCTGGCTGACCTTTATATTTTTAAACGTGAACCGCGTTTTTTAGGGCCGCTGGTACCTATTCCGGCTATGCACCAGGTa                                                                                                                  >  1:366238/1‑140 (MQ=255)                      tACCGTCACCGAAAGATATGCTGTCAGTACGTATTCGTGAATTTGCCGCCCGTTTCGGTGCGCTGGCTGACCTTTATATTTTTAAACGTGAACCGCGTTTTTTAGGGCCGCTGGTACCTATTCCGGCTATGCACCAGGTa                                                                                                                  >  1:314944/1‑140 (MQ=255)                      tACCGTCACCGAAAGATATGCTGTCAGTACGTATTCGTGAATTTGCCGCCCGTTTCGGTGCGCTGGCTGACCTTTATATTTTTAAACGTGAACCGCGTTTTTTAGGGCCGCTGGTACCTATTCCGGCTATGCACCAGGTa                                                                                                                  >  2:134950/1‑140 (MQ=255)                      tACCGTCACCGAAAGATATGCTGTCAGTACGTATTCGTGAATTTGCCGCCCGTTTCGGTGCGCTGGCTGACCTTTATATTTTTAAACGTGAACCGCGTTTTTTAGGGCCGCTGGTACCTATTCCGGCTATGCACCAGGTa                                                                                                                  >  1:1252527/1‑140 (MQ=255)                      tACCGTCACCGAAAGATATGCTGTCAGTACGTATTCGTGAATTTGCCGCCCGTTTCGGTGCGCTGGCTGACCTTTATATTTTTAAACGTGAACCGCGTTTTTTAGGGCCGCTGGTACCTATTCCGGCTATGCACCAGGTa                                                                                                                  >  1:1125498/1‑140 (MQ=255)                             aCCGAAAGATATGCTGTCAGTACGTATTCGTGAATTTGCCGCCCGTTTCGGTGCGCTGGCTGACCTTTATATTTTTAAACGTGAACCGCGTTTTTTAGGGCCGCTGGTACCTATTCCGGCTATGCACCAGGTACCGGAAg                                                                                                           <  1:224577/140‑1 (MQ=255)                             aCCGAAAGATATGCTGTCAGTACGTATTCGTGAATTTGCCGCCCGTTTCGGTGCGCTGGCTGACCTTTATATTTTTAAACGTGAACCGCGTTTTTTAGGGCCGCTGGTACCTATTCCGGCTATGCACCAGGTACCGGAAg                                                                                                           <  1:890042/140‑1 (MQ=255)                                gAAAGATATGCTGTCAGTACGTATTCGTGAATTTGCCGCCCGTTTCGGTGCGCTGGCTGACCTTTATATTTTTAAACGTGAACCGCGTTTTTTAGGGCCGCTGGTACCTATTCCGGCTATGCACCAGGTACCGGAAGATg                                                                                                        <  1:692228/140‑1 (MQ=255)                                 aaaGATATGCTGTCAGTACGTATTCGTGAATTTGCCGCCCGTTTCGGTGCGCTGGCTGACCTTTATATTTTTAAACGTGAACCGCGTTTTTTAGGGCCGCTGGTACCTATTCCGGCTATGCACCAGGTACCGGAAGATgc                                                                                                       <  1:176653/140‑1 (MQ=255)                                  aaGATATGCTGTCAGTACGTATTCGTGAATTTGCCGCCCGTTTCGGTGCGCTGGCTGACCTTTATATTTTTAAACGTGAACCGCGTTTTTTAGGGCCGCTGGTACCTATTCCGGCTATGCACCAGGTACCGGAAGATgcg                                                                                                      <  1:28380/140‑1 (MQ=255)                                  aaGATATGCTGTCAGTACGTATTCGTGAATTTGCCGCCCGTTTCGGTGCGCTGGCTGACCTTTATATTTTTAAACGTGAACCGCGTTTTTTAGGGCCGCTGGTACCTATTCCGGCTATGCACCAGGTACCGGAAGATgcg                                                                                                      <  2:1221165/140‑1 (MQ=255)                                  aaGATATGCTGTCAGTACGTATTCGTGAATTTGCCGCCCGTTTCGGTGCGCTGGCTGACCTTTATATTTTTAAACGTGAACCGCGTTTTTTAGGGCCGCTGGTACCTATTCCGGCTATGCACCAGGTACCGGAAGATgcg                                                                                                      <  1:1120588/140‑1 (MQ=255)                                                gTACGTATTCGTGAATTTGCCGCCCGTTTCGGTGCGCTGGCTGACCTTTATATTTTTAAACGTGAACCGCGTTTTTTAGGGCCGCTGGTACCTATTCCGGCTATGCACCAGGTACCGGAAGATGCGCAGGGGTATCccgc                                                                                        >  1:30882/1‑140 (MQ=255)                                                gTACGTATTCGTGAATTTGCCGCCCGTTTCGGTGCGCTGGCTGACCTTTATATTTTTAAACGTGAACCGCGTTTTTTAGGGCCGCTGGTACCTATTCCGGCTATGCACCAGGTACCGGAAGATGCGCAGGGGTATCccgc                                                                                        >  2:870584/1‑140 (MQ=255)                                                gTACGTATTCGTGAATTTGCCGCCCGTTTCGGTGCGCTGGCTGACCTTTATATTTTTAAACGTGAACCGCGTTTTTTAGGGCCGCTGGTACCTATTCCGGCTATGCACCAGGTACCGGAAGATGCGCAGGGGTATCccgc                                                                                        >  2:997719/1‑140 (MQ=255)                                                gTACGTATTCGTGAATTTGCCGCCCGTTTCGGTGCGCTGGCTGACCTTTATATTTTTAAACGTGAACCGCGTTTTTTAGGGCCGCTGGTACCTATTCCGGCTATGCACCAGGTACCGGAAGATGCGCAGGGGTATCccgc                                                                                        >  1:679497/1‑140 (MQ=255)                                                   cGTATTCGTGAATTTGCCGCCCGTTTCGGTGCGCTGGCTGACCTTTATATTTTTAAACGTGAACCGCGTTTTTTAGGGCCGCTGGTACCTATTCCGGCTATGCACCAGGTACCGGAAGATGCGCAGGGGTATCCCGCCGt                                                                                     <  2:182952/140‑1 (MQ=255)                                                   cGTATTCGTGAATTTGCCGCCCGTTTCGGTGCGCTGGCTGACCTTTATATTTTTAAACGTGAACCGCGTTTTTTAGGGCCGCTGGTACCTATTCCGGCTATGCACCAGGTACCGGAAGATGCGCAGGGGTATCCCGCCGt                                                                                     <  2:632985/140‑1 (MQ=255)                                                     tATTCGTGAATTTGCCGCCCGTTTCGGTGCGCTGGCTGACCTTTATATTTTTAAACGTGAACCGCGTTTTTTAGGGCCGCTGGTACCTATTCCGGCTATGCACCAGGTACCGGAAGATGCGCAGGGGTATCCCGCCGTca                                                                                   <  1:27163/140‑1 (MQ=255)                                                     tATTCGTGAATTTGCCGCCCGTTTCGGTGCGCTGGCTGACCTTTATATTTTTAAACGTGAACCGCGTTTTTTAGGGCCGCTGGTACCTATTCCGGCTATGCACCAGGTACCGGAAGATGCGCAGGGGTATCCCGCCGTca                                                                                   <  2:1252527/140‑1 (MQ=255)                                                         cGTGAATTTGCCGCCCGTTTCGGTGCGCTGGCTGACCTTTATATTTTTAAACGTGAACCGCGTTTTTTAGGGCCGCTGGTACCTATTCCGGCTATGCACCAGGTACCGGAAGATGCGCAGGGGTATCCCGCCGTCACAcc                                                                               >  1:538991/1‑140 (MQ=255)                                                         cGTGAATTTGCCGCCCGTTTCGGTGCGCTGGCTGACCTTTATATTTTTAAACGTGAACCGCGTTTTTTAGGGCCGCTGGTACCTATTCCGGCTATGCACCAGGTACCGGAAGATGCGCAGGGGTATCCCGCCGTCACAcc                                                                               >  2:64647/1‑140 (MQ=255)                                                         cGTGAATTTGCCGCCCGTTTCGGTGCGCTGGCTGACCTTTATATTTTTAAACGTGAACCGCGTTTTTTAGGGCCGCTGGTACCTATTCCGGCTATGCACCAGGTACCGGAAGATGCGCAGGGGTATCCCGCCGTCACAcc                                                                               <  2:1125498/140‑1 (MQ=255)                                                          gTGAATTTGCCGCCCGTTTCGGTGCGCTGGCTGACCTTTATATTTTTAAACGTGAACCGGGTTTTTTAGGGCCGCTGGTACCTATTCCGGCTATGCACCAGGTACGGGAAGATGCGCAGGGGTATCCCGCCGTAACAccc                                                                              <  1:397099/140‑1 (MQ=255)                                                           tGAATTTGCCGCCCGTTTCGGTGCGCTGGCTGACCTTTATATTTTTAAACGTGAACCGCGTTTTTTAGGGCCGCTGGTACCTATTCCGGCTATGCACCAGGTACCGGAAGATGCGCAGGGGTATCCCGCCGTCACACCCg                                                                             >  2:667466/1‑140 (MQ=255)                                                           tGAATTTGCCGCCCGTTTCGGTGCGCTGGCTGACCTTTATATTTTTAAACGTGAACCGCGTTTTTTAGGGCCGCTGGTACCTATTCCGGCTATGCACCAGGTACCGGAAGATGCGCAGGGGTATCCCGCCGTCACACCCg                                                                             <  2:722439/140‑1 (MQ=255)                                                            gAATTTGCCGCCCGTTTCGGTGCGCTGGCTGACCTTTATATTTTTAAACGTGAACCGCGTTTTTTAGGGCCGCTGGTACCTATTCCGGCTATGCACCAGGTACCGGAAGATGCGCAGGGGTATCCCGCCGTCACACCCGa                                                                            <  2:366238/140‑1 (MQ=255)                                                             aaTTTGCCGCCCGTTTCGGTGCGCTGGCTGACCTTTATATTTTTAAACGTGAACCGCGTTTTTTAGGGCCGCTGGTACCTATTCCGGCTATGCACCAGGTACCGGAAGATGCGCAGGGGTATCCCGCCGTCACACCCGaa                                                                           >  1:141681/1‑140 (MQ=255)                                                              aTTTGCCGCCCGTTTCGGTGCGCTGGCTGACCTTTATATTTTTAAACGTGAACCGCGTTTTTTAGGGCCGCTGGTACCTATTCCGGCTATGCACCAGGTACCGGAAGATGCGCAGGGGTATCCCGCCGTCACACCCGAAc                                                                          <  2:228277/140‑1 (MQ=255)                                                              aTTTGCCGCCCGTTTCGGTGCGCTGGCTGACCTTTATATTTTTAAACGTGAACCGCGTTTTTTAGGGCCGCTGGTACCTATTCCGGCTATGCACCAGGTACCGGAAGATGCGCAGGGGTATCCCGCCGTCACACCCGAAc                                                                          <  1:1051267/140‑1 (MQ=255)                                                                   ccgccCGTTTCGGTGCGCTGGCTGACCTTTATATTTTTAAACGTGAACCGCGTTTTTTAGGGCCGCTGGTACCTATTCCGGCTATGCACCAGGTACCGGAAGATGCGCAGGGGTATCCCGCCGTCACACCCGAACAGTTa                                                                     <  1:214479/140‑1 (MQ=255)                                                                                 gcgcTGGCTGACCTTTATATTTTTAAACGTGAACCGCGTTTTTTAGGGCCGCTGGTACCTATTCCGGCTATGCACCAGGTACCGGAAGATGCGCAGGGGTATCCCGCCGTCACACCCGAACAGTTACTTGAATTACaaaa                                                       >  2:280602/1‑140 (MQ=255)                                                                                  cgcTGGCTGACCTTTATATTTTTAAACGTGAACCGCGTTTTTTAGGGCCGCTGGTACCTATTCCGGCTATGCACCAGGTACCGGAAGATGCGCAGGGGTATCCCGCCGTCACACCCGAACAGTTACTTGAATTACAAAAg                                                      >  2:183723/1‑140 (MQ=255)                                                                                   gctggctgACCTTTATATTTTTAAACGTGAACCGCGTTTTTTAGGGCCGCTGGTACCTATTCCGGCTATGCACCAGGTACCGGAAGATGCGCAGGGGTATCCCGCCGTCACACCCGAACAGTTACTTGAATTACAAAAGa                                                     <  2:538991/140‑1 (MQ=255)                                                                                   gctggctgACCTTTATATTTTTAAACGTGAACCGCGTTTTTTAGGGCCGCTGGTACCTATTCCGGCTATGCACCAGGTACCGGAAGATGCGCAGGGGTATCCCGCCGTCACACCCGAACAGTTACTTGAATTACAAAAGa                                                     >  2:504548/1‑140 (MQ=255)                                                                                      ggctgACCTTTATATTTTTAAACGTGAACCGCGTTTTTTAGGGCCGCTGGTACCTATTCCGGCTATGCACCAGGTACCGGAAGATGCGCAGGGGTATCCCGCCGTCACACCCGAACAGTTACTTGAATTACAAAAGAAAc                                                  <  1:42885/140‑1 (MQ=255)                                                                                       gctgACCTTTATATTTTTAAACGTGAACCGCGTTTTTTAGGGCCGCTGGTACCTATTCCGGCTATGCACCAGGTACCGGAAGATGCGCAGGGGTATCCCGCCGTCACACCCGAACAGTTACTTGAATTACAAAAGAAACa                                                 <  1:369935/140‑1 (MQ=255)                                                                                         tgACCTTTATATTTTTAAACGTGAACCGCGTTTTTTAGGGCCGCTGGTACCTATTCCGGCTATGCACCAGGTACCGGAAGATGCGCAGGGGTATCCCGCCGTCACACCCGAACAGTTACTTGAATTACAAAAGAAACAAg                                               <  2:359489/140‑1 (MQ=255)                                                                                           aCCTTTATATTTTTAAACGTGAACCGCGTTTTTTAGGGCCGCTGGTACCTATTCCGGCTATGCACCAGGTACCGGAAGATGCGCAGGGGTATCCCGCCGTCACACCCGAACAGTTACTTGAATTACAAAAGAAACAAGGa                                             <  2:766956/140‑1 (MQ=255)                                                                                           aCCTTTATATTTTTAAACGTGAACCGCGTTTTTTAGGGCCGCTGGTACCTATTCCGGCTATGCACCAGGTACCGGAAGATGCGCAGGGGTATCCCGCCGTCACACCCGAACAGTTACTTGAATTACAAAAGAAACAAGGa                                             <  1:667466/140‑1 (MQ=255)                                                                                             cTTTATATTTTTAAACGTGAACCGCGTTTTTTAGGGCCGCTGGTACCTATTCCGGCTATGCACCAGGTACCGGAAGATGCGCAGGGGTATCCCGCCGTCACACCCGAACAGTTACTTGAATTACAAAAGAAACAAGGaaa                                           <  2:632469/140‑1 (MQ=255)                                                                                              tttATATTTTTAAACGTGAACCGCGTTTTTTAGGGCCGCTGGTACCTATTCCGGCTATGCACCAGGTACCGGAAGATGCGCAGGGGTATCCCGCCGTCACACCCGAACAGTTACTTGAATTACAAAAGAAACAAGGAAag                                          <  1:289754/140‑1 (MQ=255)                                                                                               ttATATTTTTAAACGTGAACCGCGTTTTTTAGGGCCGCTGGTACCTATTCCGGCTATGCACCAGGTACCGGAAGATGCGCAGGGGTATCCCGCCGTCACACCCGAACAGTTACTTGAATTACAAAAGAAACAAGGAAagt                                         >  2:808347/1‑140 (MQ=255)                                                                                                  tatTTTTAAACGTGAACCGCGTTTTTTAGGGCCGCTGGTACCTATTCCGGCTATGCACCAGGTACCGGAAGATGCGCAGGGGTATCCCGCCGTCACACCCGAACAGTTACTTGAATTACAAAAGAAACAAGGAAagtagt                                      <  1:776929/140‑1 (MQ=255)                                                                                                      tttAAACGTGAACCGCGTTTTTTAGGGCCGCTGGTACCTATTCCGGCTATGCACCAGGTACCGGAAGATGCGCAGGGGTATCCCGCCGTCACACCCGAACAGTTACTTGAATTACAAAAGAAACAAGGAAAGTAGTTATg                                  >  1:570046/1‑140 (MQ=255)                                                                                                       ttAAACGTGAACCGCGTTTTTTAGGGCCGCTGGTACCTATTCCGGCTATGCACCAGGTACCGGAAGATGCGCAGGGGTATCCCGCCGTCACACCCGAACAGTTACTTGAATTACAAAAGAAACAAGGAAAGTAGTTATgg                                 >  2:940908/1‑140 (MQ=255)                                                                                                       ttAAACGTGAACCGCGTTTTTTAGGGCCGCTGGTACCTATTCCGGCTATGCACCAGGTACCGGAAGATGCGCAGGGGTATCCCGCCGTCACACCCGAACAGTTACTTGAATTACAAAAGAAACAAGGAAAGTAGTTATgg                                 >  1:449324/1‑140 (MQ=255)                                                                                                       ttAAACGTGAACCGCGTTTTTTAGGGCCGCTGGTACCTATTCCGGCTATGCACCAGGTACCGGAAGATGCGCAGGGGTATCCCGCCGTCACACCCGAACAGTTACTTGAATTACAAAAGAAACAAGGAAAGTAGTTATgg                                 <  1:504548/140‑1 (MQ=255)                                                                                                          aaCGTGAACCGCGTTTTTTAGGGCCGCTGGTACCTATTCCGGCTATGCACCAGGTACCGGAAGATGCGCAGGGGTATCCCGCCGTCACACCCGAACAGTTACTTGAATTACAAAAGAAACAAGGAAAGTAGTTATGGATg                              <  1:947373/140‑1 (MQ=255)                                                                                                          aaCGTGAACCGCGTTTTTTAGGGCCGCTGGTACCTATTCCGGCTATGCACCAGGTACCGGAAGATGCGCAGGGGTATCCCGCCGTCACACCCGAACAGTTACTTGAATTACAAAAGAAACAAGGAAAGTAGTTATGGATg                              <  1:900798/140‑1 (MQ=255)                                                                                                                 aCCGCGTTTTTTAGGGCCGCTGGTACCTATTCCGGCTATGCACCAGGTACCGGAAGATGCGCAGGGGTATCCCGCCGTCACACCCGAACAGTTACTTGAATTACAAAAGAAACAAGGAAAGTAGTTATGGATGTAcagca                       <  1:660230/140‑1 (MQ=255)                                                                                                                     cgTTTTTTAGGGCCGCTGGTACCTATTCCGGCTATGCACCAGGTACCGGAAGATGCGCAGGGGTATCCCGCCGTCACACCCGAACAGTTACTTGAATTACAAAAGAAACAAGGAAAGTAGTTATGGATGTACAGCAGttt                   >  1:30404/1‑140 (MQ=255)                                                                                                                                gCCGCTGGTACCTATTCCGGCTATGCACCAGGTACCGGAAGATGCGCAGGGGTATCCCGCCGTCACACCCGAACAGTTACTTGAATTACAAAAGAAACAAGGAAAGTAGTTATGGATGTACAGCAGTTTTTTGTCGTTGc        <  2:167900/140‑1 (MQ=255)                                                                                                                                gCCGCTGGTACCTATTCCGGCTATGCACCAGGTACCGGAAGATGCGCAGGGGTATCCCGCCGTCACACCCGAACAGTTACTTGAATTACAAAAGAAACAAGGAAAGTAGTTATGGATGTACAGCAGTTTTTTGTCGTTGc        <  1:982235/140‑1 (MQ=255)                                                                                                                                    cTGGTACCTATTCCGGCTATGCACCAGGTACCGGAAGATGCGCAGGGGTATCCCGCCGTCACACCCGAACAGTTACTTGAATTACAAAAGAAACAAGGAAAGTAGTTATGGATGTACAGCAGTTTTTTGTCGTTGCCGtt    >  2:446888/1‑140 (MQ=255)                                                                                                                                    cTGGTACCTATTCCGGCTATGCACCAGGTACCGGAAGATGCGCAGGGGTATCCCGCCGTCACACCCGAACAGTTACTTGAATTACAAAAGAAACAAGGAAAGTAGTTATGGATGTACAGCAGTTTTTTGTCGTTGCCGtt    >  1:75443/1‑140 (MQ=255)                                                                                                                                    cTGGTACCTATTCCGGCTATGCACCAGGTACCGGAAGATGCGCAGGGGTATCCCGCCGTCACACCCGAACAGTTACTTGAATTACAAAAGAAACAAGGAAAGTAGTTATGGATGTACAGCAGTTTTTTGTCGTTGCCGtt    >  2:340500/1‑140 (MQ=255)                                                                                                                                    cTGGTACCTATTCCGGCTATGCACCAGGTACCGGAAGATGCGCAGGGGTATCCCGCCGTCACACCCGAACAGTTACTTGAATTACAAAAGAAACAAGGAAAGTAGTTATGGATGTACAGCAGTTTTTTGTCGTTGCCGtt    >  2:1166467/1‑140 (MQ=255)                                                                                                                                      ggTACCTATTCCGGCTATGCACCAGGTACCGGAAGATGCGCAGGGGTATCCCGCCGTCACACCCGAACAGTTACTTGAATTACAAAAGAAACAAGGAAAGTAGTTATGGATGTACAGCAGTTTTTTGTCGTTGCCGtttt  <  1:1166467/140‑1 (MQ=255)                                                                                                                                      ggTACCTATTCCGGCTATGCACCAGGTACCGGAAGATGCGCAGGGGTATCCCGCCGTCACACCCGAACAGTTACTTGAATTACAAAAGAAACAAGGAAAGTAGTTATGGATGTACAGCAGTTTTTTGTCGTTGCCGtttt  <  2:314944/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                         TTCAGGCTCAGTGTTATGCCGTACCGTCACCGAAAGATATGCTGTCAGTACGTATTCGTGAATTTGCCGCCCGTTTCGGTGCGCTGGCTGACCTTTATATTTTTAAACGTGAACCGCGTTTTTTAGGGCCGCTGGTCCCTATTCCGGCTATGCACCAGGTACCGGAAGATGCGCAGGGGTATCCCGCCGTCACACCCGAACAGTTACTTGAATTACAAAAGAAACAAGGAAAGTAGTTATGGATGTACAGCAGTTTTTTGTCGTTGCCGTTTT  >  NC_000913/2233362‑2233634

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑